Micro-fluidic chip fluorescence detector

Abstract

The invention provides a micro-fluidic chip fluorescence detector which comprises a shell, a rectangular hole is formed in the side surface of the shell, and a touch display screen is arranged on the shell; a groove for placing a chip to be detected, a tray for placing the groove and a motor sliding rail for placing the tray are arranged in the shell; the driving part is used for driving the motor and the to-be-detected chip to rotate so as to separate the to-be-detected sample into each detection groove of the chip; the fluorescence module is used for emitting fluorescence to the to-be-detected sample and receiving a processed and amplified fluorescence signal; the main control board is used for acquiring the processed and amplified fluorescence signal and analyzing and processing data to obtain a detection result; the temperature control module can be used for rapidly heating the sample to be detected to enable the sample to reach reaction conditions and rapidly reducing the temperature of the sample to be detected and parts in the shell; the touch display screen, the driving piece, the fluorescent module and the temperature control module are electrically connected with the main control board. The system is easy to operate, high in intelligent degree and portable.

Description

TECHNICAL FIELD

[0001] The present application relates to the microfluidic chip detection technical field, and in particular to a microfluidic chip fluorescence detector. BACKGROUND

[0002] Microfluidic technology refers to a scientific technology for precisely controlling and manipulating fluid in small scale or even micro scale. It realizes sample pre-treatment, reaction and analysis and other steps in a chip of several square centimeters, which can only be carried out in a standard laboratory, and is therefore called microfluidic chip. Compared with other fluid control devices, the microfluidic chip has greater improvement in micro scale characteristics, micro fluid contact efficiency, heat transfer efficiency, and greatly reduces reagent and sample volume consumption, which is conducive to parallel analysis in a limited environment, and can be applied to large-scale screening, sample batch processing or mobile detection and other scenes.

[0003] The microfluidic chip is a development hotspot of micro-volume full analysis system. Through combination with inorganic reaction, organic reaction and biological reaction, the whole process or part of the process of reagent sampling, sample separation, reaction, result detection is completed, and micron-level construction is made, which is integrated on a chip of several square centimeters. The technology provides multiple reaction chambers for a chip, can perform multiple detections, or analyze multiple detection targets. In the field of point-of-care testing (POCT), microfluidic technology has wide application prospect and value in a series of analysis fields such as biochemical index analysis, DNA analysis, immunoassay and toxicity detection. However, the common microfluidic detection system has the defects of complex control, low automation degree, high cost, inability to be applied on a large scale and poor portability.

[0004] Therefore, how to provide a simple operation, high intelligent degree and portable microfluidic detection method has become a technical problem to be solved by the person skilled in the art. SUMMARY

[0005] The purpose of the present application is to provide a simple operation, high intelligent degree and portable microfluidic chip fluorescence detector.

[0006] The present application provides a microfluidic chip fluorescence detector, which comprises a shell,

[0007] The side surface of the shell is provided with a rectangular hole, and the shell is provided with a touch display screen;

[0008] The shell is provided with a recess for placing a chip to be detected, a tray for placing the recess is arranged in the shell, and a motor sliding rail for placing the tray is arranged in the shell.

[0009] The shell is provided with a driving member for driving the motor and rotating the chip to be detected to separate the sample to be detected into each detection groove of the chip;

[0010] and a fluorescence module for emitting fluorescence to the sample to be detected and receiving the processed amplified fluorescence signal;

[0011] and a main control board for obtaining the processed amplified fluorescence signal and analyzing the data to obtain the detection result;

[0012] and a temperature control module capable of quickly heating the temperature of the sample to be detected to reach the reaction condition and quickly reducing the temperature of the sample to be detected and the components in the shell;

[0013] The touch display screen, the driving member, the fluorescence module, and the temperature control module are electrically connected with the main control board.

[0014] Further, the motor slide rail is placed on the slide rail support, and the tray can be placed through the rectangular hole on one side of the shell under the action of the motor slide rail to place the chip to be detected. A heating disc is arranged below the groove, and the diameter of the heating disc is smaller than the diameter of the groove. The sample to be detected is heated by the heating disc.

[0015] Further, the motor is provided with a coupling, the output shaft of the motor is connected with the coupling, the coupling is fixedly connected with a chuck, and the chuck is matched with the clamping groove at the center position of the chip to be detected.

[0016] Further, the chuck can be driven by the motor to move upwards until the clamping groove at the center of the chip to be detected is fixedly nested in the groove.

[0017] Further, the fluorescence module includes a fluorescence excitation unit, a fluorescence processing unit, and a receiving detection unit. The fluorescence excitation unit emits fluorescence to the sample to be detected under the control of the main control board. The fluorescence processing unit performs light filtering and amplification processing on the outgoing fluorescence formed after the sample to be detected is irradiated with fluorescence. The receiving detection unit receives the processed amplified fluorescence signal, and the receiving detection unit sends the received fluorescence signal to the main control board.

[0018] Further, the temperature control module includes a fan, a liquid pump, and a copper pipe arranged on one side of the shell. The fan is electrically connected with the main control board, the liquid pump is electrically connected with the main control board, the liquid pump is provided with a bidirectional heating rod, the liquid pump delivers liquid to the copper pipe to realize the functions of heating and heat dissipation.

[0019] Further, the shell is further provided with a bottom plate, and the driving member, the motor, and the fluorescence module are mounted on the bottom plate.

[0020] Further, the shell comprises an upper shell and a lower shell connected together, facilitating installation and disassembly.

[0021] Compared with the prior art, the present application has the following advantages:

[0022] The microfluidic chip fluorescence detector is provided with a rectangular hole on one side of the shell, a motor sliding rail is arranged in the shell, a tray provided with a groove is arranged on the motor sliding rail, the tray can be placed into the groove through the rectangular hole under the sliding of the motor sliding rail, the chip loaded with the sample to be detected is placed into the groove, the tray is pushed back, and then the automatic detection can be realized by operating the touch display screen, so that the operation is convenient and simple.

[0023] The microfluidic chip fluorescence detector has small volume and good portability. DETAILED DESCRIPTION

[0024] Figure 1 It is a schematic diagram of the overall structure of the present application;

[0025] Figure 2 It is an exploded view of the overall structure of the present application;

[0026] Figure 3 It is a side perspective view of the overall structure of the present application;

[0027] Figure 4 It is a schematic diagram of the structure of the chip to be detected in the present application;

[0028] REFERENCE SIGNS:

[0029] 1.1, upper shell; 1.2, lower shell; 2, touch display screen; 3, main control board; 4, groove; 5, tray; 6, motor sliding rail; 7, heating disc; 8, sliding rail support; 9, chuck; 10, motor; 11, fluorescence module; 12, temperature control module; 13, driving member; 14, rectangular hole; 15, bottom plate; 16, chip to be detected; 16.1, clamping groove. DETAILED DESCRIPTION

[0030] The technical solutions in the embodiments of the present application will be described clearly and completely below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0031] As shown in the drawings, Figures 1 to 4 the present application shows a microfluidic chip fluorescence detector, which comprises a shell,

[0032] The right side of the shell is provided with a rectangular hole 14, and the shell is provided with a touch display screen 2;

[0033] The housing has a groove 4 for placing the chip to be tested 16, a tray 5 for placing the groove 4, and a motor slide rail 6 for placing the tray 5.

[0034] The housing also includes a drive unit 13, which drives the motor 10 and the chip under test 16 to rotate so that the sample under test is separated and enters each detection slot of the chip; a fluorescence module 11, which emits fluorescence to the sample under test and receives the processed and amplified fluorescence signal; a main control board 3, which acquires the processed and amplified fluorescence signal and analyzes the data to obtain the detection result; and a temperature control module 12, which can quickly heat the sample under test to reach the reaction conditions and can quickly reduce the temperature of the sample under test and the components inside the housing.

[0035] The touch screen 2, driver 13, fluorescent module 11, and temperature control module 12 are electrically connected to the main control board 3.

[0036] This invention presents a small and portable microfluidic chip fluorescence detector. The detector features a rectangular hole 14 on one side of its housing, and a motor slide rail 6 inside the housing. A tray 5 with a groove 4 is placed on the motor slide rail 6. The tray 5 slides through the rectangular hole 14 along the motor slide rail 6. Sixteen chips to be tested are placed into the groove 4, pushed back onto the tray, and then operated on the touchscreen display 2 to achieve automated detection. The operation is convenient and simple.

[0037] Specifically, such as Figure 2 The motor slide rail 6 is placed on the slide rail support 8. Under the action of the motor slide rail 6, the tray 5 can place the chip to be tested 16 through the rectangular hole 14. A heating plate 7 is provided below the groove 4. The diameter of the heating plate 7 is smaller than the diameter of the groove 4.

[0038] The motor 10 is equipped with a coupling, and the output shaft of the motor 10 is connected to the coupling. The coupling is fixedly connected to the chuck 9, and the chuck 9 is adapted to the slot 16.1 at the center of the chip 16 to be tested.

[0039] Furthermore, the chuck 9 can move upward under the drive of the motor 10 until it is fixedly nested with the center slot 16.1 of the chip to be tested 16 in the groove 4.

[0040] The fluorescence module 11 includes a fluorescence excitation unit, a fluorescence processing unit, and a receiving and detection unit. Under the control of the main control board 3, the fluorescence excitation unit emits fluorescence to the sample to be tested. The fluorescence processing unit performs filtering and amplification processing on the emitted fluorescence formed after the sample to be tested is irradiated with fluorescence. The receiving and detection unit receives the processed and amplified fluorescence signal and sends the received fluorescence signal to the main control board 3.

[0041] The temperature control module 12 comprises a fan, a liquid pump 12.1 and a copper pipe arranged at one side of the shell, the fan is electrically connected with the main control board 3, the liquid pump 12.1 is electrically connected with the main control board 3, the liquid pump 12.1 is internally provided with a bidirectional heating refrigeration rod and can deliver heating liquid or cooling liquid to the copper pipe, so as to realize rapid heating of the sample to be detected and rapid heat dissipation of the sample to be detected and the parts in the shell.

[0042] The shell is internally provided with a bottom plate 15, the driving member 13, the motor 10 and the fluorescence module 11 are installed on the bottom plate 15.

[0043] The shell comprises a split-connected upper shell 1.1 and a lower shell 1.2.

[0044] Working principle: in the specific detection, the sample to be detected is added into the detection chip, then the icon of moving out the tray is touched on the touch display screen 2, the detection chip 16 is placed into the groove 4, then the icon of pushing back the tray is touched on the touch display screen 2, and then the icon of starting detection is touched, that is, the automatic detection is started. In the detection, the chuck 9 is lifted to be nested with the center clamping groove 16.1 of the detection chip 16 by the main control board 3 driving the motor 10, the heating disc 7 heats the detection chip 16, at the same time, the bidirectional heating refrigeration rod in the liquid pump 12.1 starts to heat the liquid and deliver the heated liquid to the copper pipe, so that the detection chip 16 is in the constant temperature control, the motor 10 is controlled to rotate by the main control board 3, so as to drive the chuck 9 to rotate, thereby driving the detection chip 16 to rotate, the sample to be detected in the detection chip 16 is separated and enters the detection groove under the action of the centrifugal force, after the sample to be detected completely enters the detection groove and the temperature of the detection groove of the detection chip 16 reaches the reaction requirement, the fluorescence excitation unit starts to emit the fluorescence with a wavelength of 470nm, the emitted fluorescence excites the fluorescence of the sample to be detected in the detection groove, the emitted fluorescence passes through the fluorescence processing unit and is received by the receiving detection unit, after the receiving, the data is transmitted to the main control board 3, the main control board 3 analyzes and processes the received data, obtains the detection judgment result, and displays the detection structure through the touch display screen 2, after the detection is completed, the bidirectional heating refrigeration rod in the liquid pump 12.1 starts to deliver the cooling liquid to the copper pipe, so as to dissipate the heat of the sample to be detected and the parts in the shell.

[0045] Therefore, the application has the advantages of simple operation, high intelligent degree, no special requirement for the operation level of the operator, high efficiency of fully realizing the automation from centrifugation to detection of the chip by only adding the sample to be detected into the chip, small volume, light weight and convenience in carrying.

[0046] It should be noted that all the direction indications (such as up, down, left, right, front, back, inner, outer, and the like) in the embodiments of the present application are only used to explain the relative position relationship, movement condition and the like between components in a certain specific posture (as shown in the drawings), and if the specific posture is changed, the direction indications will also be changed accordingly.

[0047] In the present application, unless otherwise explicitly specified and limited, the terms "mounting", "connecting", "fixing" and the like should be understood in a broad sense, for example, can be fixed connection, or detachable connection, or integrated; can be mechanical connection, or electrical connection; can be directly connected, or indirectly connected through an intermediate medium; can be the internal communication of two elements or the interaction relationship between two elements. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0048] In addition, it should also be understood that when the terms "comprising" and / or "including" are used in the specification, it means that there is a feature, step, operation, device, component and / or combination thereof.

[0049] The above is only the preferred embodiment of the present application, and does not limit the implementation and protection scope of the present application. Those skilled in the art should be able to realize that any equivalent replacement and obvious changes made by applying the content of the present application should be included in the protection scope of the present application.

Claims

1. A microfluidic chip fluorescence detector, comprising a shell, characterized in that: a rectangular hole (14) is arranged on the side of the shell, and a touch display screen (2) is arranged on the shell; a recess (4) for placing a chip to be detected (16) is arranged in the shell, a tray (5) for placing the recess (4) is arranged in the shell, and a motor sliding rail (6) for placing the tray (5) is arranged in the shell; a driving member (13) is arranged in the shell, the driving member (13) is used to drive the motor (10) and the chip to be detected (16) to rotate so that the sample to be detected is separated into each detection groove of the chip; a fluorescence module (11) is arranged, which is used to emit fluorescence to the sample to be detected and receive the processed and amplified fluorescence signal; a main control board (3) is arranged, which acquires the processed and amplified fluorescence signal and analyzes and processes the data to obtain a detection result; a temperature control module (12) is arranged, which can quickly heat the temperature of the sample to be detected to reach the reaction condition and quickly reduce the temperature of the sample to be detected and the components in the shell; the touch display screen (2), the driving member (13), the fluorescence module (11), and the temperature control module (12) are electrically connected with the main control board (3) respectively.

2. The microfluidic chip fluorescence detector according to claim 1, characterized in that: The motor sliding rail (6) is placed on a sliding rail support (8), the tray (5) can place the chip to be detected (16) through the rectangular hole (14) under the action of the motor sliding rail (6), a heating disc (7) is arranged below the recess (4), and the diameter of the heating disc (7) is smaller than the diameter of the recess (4).

3. The microfluidic chip fluorescence detector according to claim 1, characterized in that: The motor (10) is provided with a shaft coupling, the output shaft of the motor (10) is connected with the shaft coupling, the shaft coupling is fixedly connected with a chuck (9), and the chuck (9) is matched with a clamping groove (16.1) at the center position of the chip to be detected (16).

4. The microfluidic chip fluorescence detector according to claim 2 or 3, characterized in that: The chuck (9) can move upwards until being fixedly nested with the center clamping groove (16.1) of the chip to be detected (16) in the recess (4) under the driving of the motor (10).

5. The microfluidic chip fluorescence detector according to claim 1, characterized in that: The fluorescence module (11) comprises a fluorescence excitation unit, a fluorescence processing unit, and a receiving and detecting unit, the fluorescence excitation unit emits fluorescence to the sample to be detected under the control of the main control board (3), the fluorescence processing unit performs light filtering and amplification processing on the outgoing fluorescence formed after the sample to be detected is irradiated with fluorescence, the receiving and detecting unit receives the processed and amplified fluorescence signal, and the receiving and detecting unit sends the received fluorescence signal to the main control board (3).

6. The microfluidic chip fluorescence detector according to claim 1, characterized in that: The temperature control module (12) comprises a fan, a liquid pump (12.1), and a copper pipe arranged on one side of the shell, the fan is electrically connected with the main control board (3), the liquid pump (12.1) is electrically connected with the main control board (3), and a bidirectional heating refrigeration rod is arranged in the liquid pump (12.1).

7. The microfluidic chip fluorescence detector according to claim 1, characterized in that: A bottom plate (15) is further arranged in the shell, and the driving member (13), the motor (10), and the fluorescence module (11) are mounted on the bottom plate (15).

8. The microfluidic chip fluorescence detector according to claim 1, characterized in that: The housing comprises an upper housing (1.1) and a lower housing (1.2) which are connected together.

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