Multifunctional test device for continuously collecting root exudates

Abstract

The invention belongs to the technical field of experimental devices. In order to solve the problems that an existing test device for collecting root exudates is low in experiment precision and small in application range, the multifunctional test device for continuously collecting the root exudates comprises two test mechanisms, and each test mechanism comprises a culture frame, a culture basin, a root support and a root exudates collecting assembly. The plant and the root support are integrally taken out of the culture pot through the root exudates collecting assembly, then the plant root and root exudates in culture soil on the surface of the plant root are washed through eluent, all the root exudates can be collected, the plant root exudates can be more sufficiently collected through the test mechanism, and the test efficiency is improved. The experiment precision is high, plants can be subjected to water culture through the root exudates collecting assembly, and use is flexible.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of experimental devices, and particularly relates to a multifunctional experimental device for continuously collecting root exudates. BACKGROUND

[0002] Root exudates are all substances secreted by roots in an active or passive manner during the growth of plants. Plant root exudates are a general term for organic and inorganic substances released by plants into the surrounding medium through roots. In a broad sense, it includes exudates, secretions, mucilaginous substances and decomposed and shed substances. In a narrow sense, it refers to soluble organic substances, mainly including sugars, organic acids, amino acids and the like. The synthesis and secretion process of root exudates is an important way to realize the interaction between plants and rhizosphere environment. Not only can it improve the properties of rhizosphere soil and regulate the community composition of rhizosphere microorganisms, but also plants can feedback regulate growth by sensing root exudates and changes in rhizosphere microecology.

[0003] The patent with publication number CN117990420A discloses a multifunctional experimental device for continuously collecting root exudates, which comprises a culture platform, a cultivation barrel, a liquid inlet component, a collection box, a collection bottle and a control component. The cultivation barrel comprises a barrel body, a quartz sand filling layer, a collection box, a collection chamber, an annular support, an annular net and a circular protective net. When in use, root exudates of a seedling body pass through the annular net and the circular protective net to the inside of the collection chamber, and at the same time, the roots of the seedling body can also pass through the annular net and the circular protective net to the inside of the annular net and the circular protective net, so that the roots of the seedling body directly contact the collection chamber. A collection head is arranged in the collection chamber, and a collection pipe is connected to the collection head, so that the collection of root exudates can be directly performed through the collection head and the collection pipe.

[0004] However, in the use of the above technical solution, the root exudates of the seedling body are mixed with the quartz sand, so only a small amount of root exudates flows downward to the collection chamber, that is, the above technical solution cannot collect all root exudates, resulting in inaccurate collection data of root exudates, low experimental precision, and the above technical solution can only collect root exudates of seedlings requiring sand culture, and cannot collect root exudates of plants requiring soil culture and water culture, so the application range is small. SUMMARY

[0005] The present application aims to overcome the problems of low experimental precision and small application range of the existing experimental device for collecting root exudates.

[0006] In order to achieve the above object, the technical idea adopted by the present application is: a multifunctional continuous root exudate collecting test device is provided, which comprises two test mechanisms, each of which comprises a culture rack, a culture pot, a root support and a root exudate collecting assembly. The plant and the root support are taken out of the culture pot through the root exudate collecting assembly, and then the root exudate in the culture soil on the surface of the plant root and the culture soil is washed with an eluent to collect all the root exudate. The test mechanism provided by the present application can more fully collect the root exudate of the plant, has high experimental precision, and can also use the root exudate collecting assembly to hydroponically cultivate the plant, which is flexible to use.

[0007] Based on the above technical idea, the technical scheme adopted by the present application is: A multifunctional continuous root exudate collecting test device comprises a culture rack and two culture pots, and further comprises two test mechanisms respectively connected with the culture pots, each of which comprises: a root support arranged in the culture pot; a root exudate collecting assembly arranged on the culture rack and communicated with an eluent containing cavity in the culture rack; a root exudate collecting box arranged on the culture rack and connected with the root exudate collecting assembly.

[0008] In order to facilitate the removal of the plant from the culture pot and the elution of the root exudate of the plant, in the above technical scheme, preferably, the root exudate collecting assembly comprises: a root traction unit rotatably connected with the culture rack and connected with the root support; a root elution unit located between the root traction unit and the culture pot and connected with the root exudate collecting box.

[0009] In order to avoid damaging the root of the plant during the process of removing the plant from the culture pot, the root traction unit comprises: a rotating plate rotatably connected with the culture rack; a lifting rod vertically arranged on the bottom surface of the rotating plate; a magnet horizontally arranged at the bottom of the lifting rod a power coil arranged on the upper surface of the root support and in contact with the magnet.

[0010] In order to facilitate the elution of the root exudate of the plant, the root elution unit further comprises: an elution box located above the culture pot and connected with the root exudate collecting box; a lifting plate arranged in the elution box and slidably connected with the elution box A transverse telescopic rod is arranged in the root exudate collecting box and connected with the elution box.

[0011] In order to facilitate the switching of the plant from the soil culture mode to the water culture mode, the root elution unit is further limited in the technical solution, and the root elution unit further comprises: A first electromagnetic plate is arranged on the bottom surface inside the elution box. A second magnetic plate is arranged on the bottom surface of the lifting plate and is magnetically connected with the first electromagnetic plate.

[0012] In order to improve the stability of the plant during the moving process, the root support is further limited in the technical solution, and the root support comprises: An upper collar is provided with a groove on the upper surface. A lower collar is connected with the upper collar through a plurality of vertical rods. A limiting magnetic rod is arranged in the groove of the upper collar. A clamping magnetic rod is arranged in the groove of the upper collar and is magnetically connected with the limiting magnetic rod.

[0013] In order to prevent the plant from shaking during the experiment, the limiting magnetic rod is further limited in the technical solution, and the limiting magnetic rod comprises a limiting part and a magnetic part rotatably connected with the limiting part; the magnetic part is slidably connected with the bottom surface of the groove of the upper collar.

[0014] In order to facilitate the experimenters to obtain real-time soil humidity, and at the same time, avoid the interference of the electronic sensor on the salt content of the culture soil, the bottom surface of each culture pot is provided with a soil humidity measuring assembly; the soil humidity measuring assembly comprises: A bearing plate is connected with the root support on the upper surface. A bearing rod is connected with the bearing plate on the upper end and located outside the culture pot after penetrating through the culture pot on the lower end. A measuring spring is located in the culture pot and sleeved with the bearing rod.

[0015] In order to more conveniently move the plant out of the culture pot, the bottom surface of each culture pot is further provided with a soil loosening assembly, and the soil loosening assembly comprises: A rotating ring is rotatably connected with the bearing plate. A plurality of soil loosening rods are vertically arranged on the upper surface of the rotating ring. A rotating handle is connected with the rotating ring on the upper end and located outside the culture pot after penetrating out of the culture pot on the lower end.

[0016] In order to facilitate the water culture of the plant or the elution of the root exudate, the culture rack is further limited in the technical solution, and the culture rack is further provided with a nutrient solution storage cavity and a soil culture watering pipeline; one end of the soil culture watering pipeline is communicated with the nutrient solution storage cavity, and the other end is communicated with the pipeline arranged in the root support. Advantages

[0017] One, the culture pot, root system support, root exudate collection assembly and hydroponic pipeline are combined to switch the culture mode of the plant, that is, the multifunctional test device for continuously collecting root exudates can be used for experiments on plants needing hydroponics, and can also be used for experiments on plants needing soil culture and sand culture, so that the application range is wide, and experimenters can adjust one test mechanism to soil culture mode and another test mechanism to hydroponics mode to compare the root exudates of the same plant under different culture modes, or adjust both test mechanisms to soil culture mode or hydroponics mode to plant different plants or irrigate different water amounts, respectively, for experiments. In addition, when the soil culture mode and the hydroponics mode need to be switched, the culture mode can be switched through the device, without manual intervention, so that the errors caused by manual operation are reduced.

[0018] Two, the root exudate collection assembly is arranged, the plant can be removed from the culture pot as a whole, and the root exudates in the plant roots and the surface soil of the plant roots are washed with an eluent to collect the root exudates, so that the experimental accuracy is high.

[0019] Three, the soil loosening assembly and the root system support are combined to loosen the soil of the plant before the plant is removed from the culture pot, so that the plant roots are not damaged.

[0020] Four, the culture pot and the soil humidity measuring assembly are combined, without the aid of external devices, and only by observing the deformation variable, the soil culture barrel can be supplemented with water in time, which avoids the problem that the traditional measurement method relies on electronic sensors, which is not only high in cost and susceptible to soil salt interference, but also needs frequent maintenance and calibration in long-term experiments, and is difficult to apply in field scenes without power supply.

[0021] Five, the first electromagnetic plate, the second magnetic plate and the lifting plate are combined to lift the lifting plate to communicate the lifting plate with the eluent containing cavity to elute the exudates on the plant roots, or to communicate the lifting plate with the nutrient solution storage cavity to hydroponic the plant.

[0022] Six, the nutrient solution storage cavity, the soil irrigation pipeline and the root system support are combined to irrigate the plant, which is simple and convenient to operate. DETAILED DESCRIPTION

[0023] In order to make the technical solutions in the embodiments of the present application or the prior art clearer, the accompanying drawings needed in the embodiments or prior art description will be briefly introduced. Obviously, the accompanying drawings in the following description only aim to some embodiments of the present application, and for those skilled in the art, other drawings can be obtained without creative effort based on these drawings.

[0024] Figure 1 A structural schematic diagram of a multifunctional test device for continuously collecting root exudates provided for the embodiment 1 is shown in the figure. Figure 2 A structural schematic diagram of the device shown in the figure is shown in the figure. Figure 1 Figure 3 A structural schematic diagram of the device shown in the figure is shown in the figure. Figure 1 A structural schematic diagram of the device shown in the figure is shown in the figure. Figure 4 A structural schematic diagram of the device shown in the figure is shown in the figure. Figure 3 A structural schematic diagram of the device shown in the figure is shown in the figure. Figure 5 Figure 1 A structural schematic diagram of the device shown in the figure is shown in the figure. Figure 6 A structural schematic diagram of the device shown in the figure is shown in the figure. Figure 1 A structural schematic diagram of the device shown in the figure is shown in the figure. Figure 7 Figure 6 A structural schematic diagram of the device shown in the figure is shown in the figure. Figure 8 A structural schematic diagram of the device shown in the figure is shown in the figure. Figure 1 A structural schematic diagram of the device shown in the figure is shown in the figure. Figure 9 Figure 8 A structural schematic diagram of the device shown in the figure is shown in the figure. Figure 10 A structural schematic diagram of the device shown in the figure is shown in the figure. Figure 8 A structural schematic diagram of the device shown in the figure is shown in the figure. Figure 11 Figure 10 A structural schematic diagram of the device shown in the figure is shown in the figure. Figure 12 A structural schematic diagram of the device shown in the figure is shown in the figure. Figure 11 A structural schematic diagram of the device shown in the figure is shown in the figure. Figure 13 Figure 10 A structural schematic diagram of the device shown in the figure is shown in the figure. Figure 14 A structural schematic diagram of the device shown in the figure is shown in the figure. Figure 1 A structural schematic diagram of the device shown in the figure is shown in the figure. Figure 15 Figure 14 A structural schematic diagram of the device shown in the figure is shown in the figure. Figure 16 A structural schematic diagram of the device shown in the figure is shown in the figure. Figure 15 A structural schematic diagram of the device shown in the figure is shown in the figure.​​​​​​​Figure 17 is a structural schematic diagram of the extraction assembly; Figure 18 is a structural schematic diagram of the root system support; Figure 19 is a structural schematic diagram of the root system elution unit; Figure 20 is a structural schematic diagram of the culture pot.

[0025] 1, root system support; 11, upper collar; 12, lower collar; 13, limiting magnetic attraction rod; 13a, limiting part; 13b, magnetic attraction part; 14, clamping magnetic attraction rod; 15, vertical rod; 2, root system secretion collection box; 3, culture shelf; 31, elution liquid containing cavity; 32, nutrient solution storage cavity; 33, soil culture irrigation pipeline; 34, elution pump; 35, irrigation pump; 4, culture pot; 5, root system traction unit; 51, rotating plate; 52, lifting rod; 53, magnet; 54, energized coil; 6, root system elution unit; 61, elution box; 62, lifting plate; 63, horizontal telescopic rod; 64, first electromagnetic plate; 65, second magnetic attraction plate; 7, soil humidity measurement assembly; 71, bearing plate; 72, bearing rod; 73, measurement spring; 8, soil loosening assembly; 81, rotating ring; 82, soil loosening rod; 83, rotating handle; 9, plant. DETAILED DESCRIPTION

[0026] In order to make the purpose, technical scheme and advantages of the embodiments of the present application clearer, the technical scheme in the embodiments of the present application will be described clearly and completely below with reference to the drawings in the embodiments of the present application.

[0027] In the description of the present application, it should be understood that the orientation or positional relationship indicated by the terms "length direction", "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", "axial direction", "radial direction", "circumferential direction" and the like is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present application and simplifying the description, and does not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation on the present application. In addition, the features with "first", "second" can be explicitly or implicitly included one or more of the features. In the description of the present application, unless otherwise specified, the meaning of "a plurality of" is two or more.

[0028] In the description of the present application, it should be noted that unless otherwise expressly specified and limited, the terms "mounting", "connection", "connecting" should be understood in a broad sense, for example, it can be fixed connection, or detachable connection, or integrally connected; it can be mechanical connection, or electrical connection; it can be directly connected, or indirectly connected through an intermediate medium, or the internal communication of two elements. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0029] The inventor found that in the use of the current experimental device for collecting root exudates, the root exudates of the seedling body will mix with quartz sand, so only a small amount of root exudates flows downward into the collection chamber, that is, the above technical solution cannot collect all root exudates, resulting in inaccurate root exudate collection data and low experimental precision. In addition, the above technical solution can only collect root exudates of seedlings that need sand culture, and cannot collect root exudates of plants that need soil culture and water culture, and the application range is small.

[0030] Based on the above findings, the present application provides a multifunctional continuous root exudate collection experimental device, which comprises a root exudate collection assembly, a root support 1, a root exudate collection box 2, a culture shelf 3, a culture pot 4, a root traction unit 5, a root elution unit 6, a soil humidity measuring assembly 7 and a soil loosening assembly 8. The present application has the following design, which makes the experimental results of the whole device more accurate and the application range wider, specifically: 1. The root exudate collection assembly can not only soil culture the plant 9, but also remove the plant 9 from the culture pot 4 during soil culture, thereby eluting the root exudates in the culture soil attached to the roots and the surface of the plant 9, improving the data acquisition accuracy of the amount of root exudates, and can also water culture the plant 9, and can also elute the plant 9 during water culture, with high experimental precision. Furthermore, the root exudate collection assembly can also water culture the plant 9, and has a wide application range. 2. The soil loosening assembly 8 can loosen the culture soil before the root traction unit 5 removes the plant 9 from the culture pot 4, avoiding damage to the roots of the plant 9 when the plant 9 is removed. 3. The soil humidity measuring assembly 7 can directly reflect the soil humidity, avoiding the problem that the traditional measurement method relies too much on electronic sensors, which not only has high cost, is easily disturbed by soil salt, and needs frequent maintenance and calibration in long-term experiments, and is difficult to apply in field scenes without power supply.

[0031] Embodiment 1 The present embodiment provides a multifunctional continuous root exudate collection experimental device, which comprises a root exudate collection assembly, a root support 1, a root exudate collection box 2, a culture shelf 3, a culture pot 4, a root traction unit 5, a root elution unit 6, a soil humidity measuring assembly 7 and a soil loosening assembly 8. Figures 1-20As shown, it comprises a culture rack 3, two culture pots 4, and two test mechanisms corresponding to the two culture pots 4, each of which comprises a root exudate collection assembly, a root support 1, a root exudate collection tank 2, a root traction unit 5, a root elution unit 6, a soil humidity measuring assembly 7, and a soil loosening assembly 8.

[0032] The culture rack 3 is internally provided with an elution liquid accommodating cavity 31, a nutrient solution storage cavity 32, a soil culture watering pipeline 33, and a cavity for mounting the root exudate collection tank 2; one end of the soil culture watering pipeline 33 is in communication with the nutrient solution storage cavity 32, and the other end is in communication with the culture pot 4; the root exudate collection tank 2 is connected with the root exudate collection assembly.

[0033] Specifically, the culture rack 3 is provided with two limiting grooves for mounting the corresponding culture pots 4 respectively, and the two test mechanisms are mounted on the same side of the corresponding culture pots 4 respectively so as to perform a control experiment.

[0034] The culture pot 4 is of a cylindrical structure and is fixedly connected with the culture rack 3; specifically, the culture pot 4 is provided on its bottom surface with a central through hole for connecting with the soil humidity measuring assembly 7 and an arc-shaped hole for connecting with the soil loosening assembly 8.

[0035] The soil humidity measuring assembly 7 comprises a bearing plate 71, a bearing rod 72, and a measuring spring 73.

[0036] Specifically, the bearing plate 71 is slidingly arranged in the culture pot 4; The upper end of the bearing rod 72 penetrates into the interior of the culture pot 4 and is fixedly connected with the bearing plate 71, and the lower end penetrates through the central through hole of the culture pot 4 and is located below the culture pot 4, so that the experimenter can determine the soil humidity according to the length of the bearing rod 72 protruding out; The measuring spring 73 is arranged in the interior of the culture pot 4 and is sleeved with the bearing rod 72, so that when the bearing plate 71 moves downward under the pressure of the plant 9, culture soil, etc., the measuring spring 73 is compressed.

[0037] In actual application, the real-time soil humidity of the culture pot 4 is obtained by using the soil humidity measuring assembly 7, including the following steps: Step one: before watering the plant 9, put the quantitative culture soil into the culture pot and record the length x1 of the bearing rod 72 protruding out of the culture pot 4; Step two: water the plant 9 with a quantitative amount of water; Step three: according to the formula: soil humidity (%) = quantitative water weight / quantitative culture soil weight x 100%, the soil humidity of the culture pot 4 at the beginning of the experiment can be obtained; Step four: start the test; As the experiment proceeds, the moisture in the culture pot 4 evaporates continuously; the experimenter checks the length of the load rod 72 leaking out of the culture pot 4 at any time, and records the length x2 of the load rod 72 leaking out of the culture pot 4; Step six: according to the formula: x=x1-x2, the spring deformation x is obtained; Step seven: according to the Hooke's law formula: F=kx, the spring force F, i.e. the weight of the remaining water in the culture pot 4, is obtained; wherein F is the spring force, x is the spring deformation, and k is the stiffness coefficient of the measuring spring 73; Step eight: according to the formula: real-time soil moisture (%) = weight of remaining water in culture pot 4 / dry soil weight x 100%, the real-time soil moisture of the culture pot 4 can be obtained; wherein the dry soil weight is the weight of the quantitative culture soil in step one.

[0038] Wherein, in order to facilitate the experimenter to quickly determine the length of the load rod 72 leaking out of the culture pot 4, scale lines are provided on the load rod 72 for the experimenter to record.

[0039] The load plate 71 is rotationally connected with the soil loosening assembly 8; the soil loosening assembly 8 includes a rotating ring 81, a soil loosening rod 82 and a rotating handle 83.

[0040] Specifically, the rotating ring 81 is located in the annular groove on the upper surface of the load plate 71, and the upper surface of the load plate 71 is provided with a groove for rotationally connecting with the rotating ring 81; A plurality of soil loosening rods 82 are vertically fixedly arranged on the upper surface of the rotating ring 81, and the plurality of soil loosening rods 82 are arranged in equal intervals on the circumference of the rotating ring 81 as the center; The rotating handle 83 is vertically fixedly arranged on the bottom surface of the rotating ring 81, and the lower end of the rotating handle 83 penetrates out of the culture pot 4 and is located below the culture pot 4, so that the experimenter can rotate the rotating handle 83 back and forth after grasping it, and the rotating handle 83 drives the soil loosening rod 82 to rotate back and forth through the rotating ring 81, so as to loosen the culture soil in the culture pot 4.

[0041] The root system support 1 is fixedly arranged on the upper surface of the load plate 71, and is used to assist the growth of the root system of the plant 9, so that the root system of the plant 9 is wound on the root system support 1 during growth, so that the plant 9 and the root system support 1 are moved out of the culture pot 4 synchronously after loosening the soil.

[0042] The root system support 1 includes an upper collar 11, a lower collar 12, a plurality of limiting magnetic rods 13, a plurality of clamping magnetic rods 14 and a plurality of vertical rods 15.

[0043] Specifically, the upper sleeve ring 11 and the lower sleeve ring 12 are arranged in an up-down manner; wherein the upper surface of the upper sleeve ring 11 is provided with a groove for matching connection with the root system traction unit 5, and the upper surface of the upper sleeve ring 11 is also provided with a linear sliding groove for mounting the limiting magnetic rod 13 and the clamping magnetic rod 14; the inner part of each linear sliding groove is respectively provided with one limiting magnetic rod 13 and one clamping magnetic rod 14.

[0044] The limiting magnetic rod 13 comprises a limiting part 13a and a magnetic part 13b rotationally connected to the limiting part 13a; Specifically, the limiting part 13a is a square sheet structure, and the end close to the magnetic part 13b is provided with a chamfer; The magnetic part 13b is rotationally connected to the limiting part 13a at one end, and the other end passes through the upper sleeve ring 11 and is located close to the plant 9, and the end of the magnetic part 13b close to the plant 9 is also provided with a chamfer; wherein the lower surface of the magnetic part 13b is fixedly provided with a sliding block, and the inner bottom surface of the linear sliding groove of the upper sleeve ring 11 is provided with a sliding groove matched with the sliding block for sliding connection, so that the magnetic part 13b can only slide in the horizontal direction.

[0045] In the initial state, the connection part of the limiting part 13a and the magnetic part 13b is located in the linear sliding groove of the upper sleeve ring 11, and the end of the limiting part 13a away from the magnetic part 13b is located in the limiting groove opened on the inner wall of the culture pot 4, so that through the cooperation of the limiting groove and the limiting part 13a, the whole root system support 1 can be prevented from tilting and shaking; When the root system traction unit 5 is in contact with the upper surface of the upper sleeve ring 11, the root system traction unit 5 pushes the magnetic part 13b outward along the chamfered surface of the magnetic part 13b, so that the connection part of the limiting part 13a and the magnetic part 13b is away from the linear sliding groove of the upper sleeve ring 11, so that the limiting part 13a is turned down under the action of gravity, so as to leave the limiting groove on the inner wall of the culture pot 4, so that the root system traction unit 5 can move the plant 9 and the root system support 1 out of the culture pot 4.

[0046] Each clamping magnetic rod 14 is located in the corresponding linear sliding groove, and one end of each clamping magnetic rod 14 is magnetically connected to the corresponding magnetic part 13b, and the other end passes out of the side of the upper sleeve ring 11 and is located close to the plant 9, and the end of each clamping magnetic rod 14 close to the plant 9 is also provided with an arc-shaped clamping rod to better contact and clamp the stem of the plant 9; Wherein, similar to the magnetic part 13b, the lower surface of each clamping magnetic rod 14 is fixedly provided with a sliding block, and the inner bottom surface of the linear sliding groove of the upper sleeve ring 11 is provided with a sliding groove matched with the sliding block of the clamping magnetic rod 14 for sliding connection, so that each clamping magnetic rod 14 can only slide in the horizontal direction; Specifically, the end of the clamping magnetic rod 14 magnetically connected with the magnetic attraction part 13b is provided with a chamfer, so that the root system traction unit 5 moves the clamping magnetic rod 14 to the side close to the stem of the plant 9 along the chamfered inclined surface of the clamping magnetic rod 14, so as to clamp and fix the plant 9, so that the plant 9 does not shake and fall off during the process of moving out the plant 9 from the culture pot 4 by the moving-out assembly 6.

[0047] The upper end of each vertical rod 15 is fixedly connected with the bottom surface of the upper sleeve ring 11, the lower end is fixedly connected with the upper surface of the bearing plate 71, and the middle part of the vertical rod 15 is fixedly connected with the lower sleeve ring 12; wherein the inside of each vertical rod 15 is provided with an elution channel, so as to elute the secretion on the root system of the plant 9.

[0048] The root system traction unit 5 comprises a rotating plate 51, a lifting rod 52, a magnet 53 and a power coil 54.

[0049] Specifically, the rotating plate 51 is located above the culture pot 4 and is rotationally connected with the culture rack 3. The lifting rod 52 is fixedly arranged on the bottom surface of the rotating plate 51, and the movable end of the lifting rod 52 is fixedly connected with the magnet 53. It should be noted that the lifting rod 52 provided by the embodiment of the present application is a prior art, and the embodiment of the present application does not improve it. For example, the lifting rod 52 is an electric push rod. The power coil 54 is fixedly arranged in the groove of the upper sleeve ring 11, so as to be magnetically connected with the magnet 53.

[0050] In actual application, in order to improve the stability during the process of moving out the plant 9, a plurality of lifting rods 52 and a plurality of magnets 53 can be arranged on the bottom surface of the rotating plate 51. The embodiment of the present application is illustrated by taking the example that two lifting rods 52 and two magnets 53 are arranged on the bottom surface of the rotating plate 51.

[0051] The working principle of the root system traction unit 5 provided by the embodiment of the present application is as follows: The experimenter manually rotates the rotating plate 51, so that the rotating plate 51 is located directly above the culture pot 4, and then starts the lifting rod 52. The lifting rod 52 drives the magnet 53 to move downward until the magnet 53 enters the linear sliding groove of the upper sleeve ring 11. At this time, the magnet 53 separates the magnetic attraction part 13b and the clamping magnetic rod 14 along the chamfered inclined surface, so that the magnetic attraction part 13b moves outward until the connecting part of the limiting part 13a and the magnetic attraction part 13b leaves the linear sliding groove of the upper sleeve ring 11, so that the limiting part 13a is flipped downward under the action of gravity, thereby leaving the limiting groove on the inner wall of the culture pot 4, so as to move the plant 9 and the root system support 1 out of the culture pot 4 by the root system traction unit 5, and move the clamping magnetic rod 14 to the side close to the stem of the plant 9, so as to clamp and fix the plant 9. Finally, the energized coil 54 is energized, so that the energized coil 54 generates a magnetic field to be magnetically connected with the magnet 53, and then the lifting rod 52 is started to make the movable end of the lifting rod 52 retreat, thereby driving the root support 1 and the plant 9 wound on the root support 1 to move out of the culture pot 4 synchronously.

[0052] The root elution unit 6 comprises an elution box 61, a lifting plate 62, a transverse telescopic rod 63, a first electromagnetic plate 64 and a second magnetic plate 65.

[0053] Specifically, the elution box 61 is slidably connected with the side of the root exudate collection tank 2, that is, one side of the elution box 61 is located inside the root exudate collection tank 2, and the other side is located outside the root exudate collection tank 2. The lifting plate 62 is slidably arranged inside the elution box 61. Specifically, the lifting plate 62 comprises an L-shaped connecting portion and a circular filter screen integrally connected with the L-shaped connecting portion, and one end of the L-shaped connecting portion away from the circular filter screen penetrates out of the elution box 61 and is located in the limiting groove of the culture rack 3. An L-shaped channel is arranged in the L-shaped connecting portion, so that the nutrient solution or the elution solution enters the elution box 61. The inner wall of the limiting groove of the culture rack 3 is provided with two through holes, one of which is communicated with the elution liquid containing cavity 31, and the other is communicated with the nutrient solution storage cavity 32. The two through holes in the limiting groove are each provided with a one-way valve. It should be noted that the one-way valve provided in the embodiment of the present application is a prior art, and the embodiment of the present application does not improve it. The transverse telescopic rod 63 is fixedly arranged on the inner side wall of the root exudate collection tank 2, and the movable end of the transverse telescopic rod 63 is fixedly connected with the side of the elution box 61, so that the elution box 61 moves synchronously with the movable end of the transverse telescopic rod 63. It should be noted that the transverse telescopic rod 63 provided in the embodiment of the present application is a prior art, and the embodiment of the present application does not improve it. For example, the transverse telescopic rod 63 is an electric push rod.

[0054] The first electromagnetic plate 64 is fixedly arranged on the inner bottom surface of the elution box 61. Specifically, the first electromagnetic plate 64 is an energized coil.

[0055] The second magnetic plate 65 is fixedly arranged on the bottom surface of the lifting plate 62.

[0056] The root elution unit 6 provided in the embodiment of the present application works in two modes: Mode one, water culture of the plant 9; Specifically, the assembly 6 drives the root support 1 and the plant 9 wound on the root support 1 to move into the elution box 61, and then the irrigation pump 35 arranged in the nutrient solution storage cavity 32 is opened, so that the nutrient solution in the nutrient solution storage cavity 32 enters the elution box 61, thereby realizing water culture of the plant 9. In the hydroponic process, the L-shaped connecting part in the lifting plate 62 blocks the liquid outlet hole of the elution box 61 to prevent the nutrient solution from flowing out.

[0057] Mode two, eluting the root exudates on the roots of the plant 9; Specifically, first, the horizontal telescopic rod 63 is started, and the horizontal telescopic rod 63 drives the elution box 61 to move to the lower side of the extraction assembly 6; Secondly, the extraction assembly 6 drives the root support 1 and the plant 9 wound on the root support 1 to move into the elution box 61; Then, the first electromagnetic plate 64 is powered, the first electromagnetic plate 64 generates a magnetic field, and the principle of same polarity repulsion is used to make the lifting plate 62 move upwards until the lifting plate 62 is located at the top of the limiting groove of the culture shelf 3, at this time, the L-shaped channel in the lifting plate 62 is in communication with another through hole on the inner wall of the limiting groove of the culture shelf 3; Finally, the elution pump 34 fixedly arranged in the elution liquid containing cavity is opened, the elution pump 34 pumps out the elution liquid, and after the elution liquid passes through the L-shaped channel in the lifting plate 62, the elution liquid enters the inside of the elution box 61; at this time, the L-shaped connecting part in the lifting plate 62 no longer blocks the liquid outlet hole of the elution box 61, so that the root exudates enter the root exudate collection box 2 along with the elution liquid, and the collection of the root exudates is realized.

[0058] It should be noted that the elution pump 34 and the irrigation pump 35 in the embodiment of the present application are prior art, and the embodiment of the present application does not improve them. For example, the elution pump 34 and the irrigation pump 35 are both water pumps with a model of QW50-15-25-2.2.

[0059] Implementation case Taking soil culture of the plant 9 as an example, the following steps are included: S1: planting the plant 9 in the culture pot 4 and adding culture soil in the culture pot 4; S2: recording the length x1 of the weight rod 72 leaking out of the culture pot 4; S3: watering the plant 9 with a fixed amount of water to start planting; During the planting process, the real-time soil moisture of the culture pot 4 can be obtained according to the formula: real-time soil moisture (%) = weight of remaining water in the culture pot 4 / dry soil weight*100%, so as to water in time; S4: collecting the root exudates of the plant 9 after a certain period of planting; specifically, this step includes the following steps: S401: the experimenter manually rotates the rotating plate 51 so that the rotating plate 51 is located directly above the culture pot 4; S402: Start the lifting rod 52, the lifting rod 52 drives the magnet 53 to move downwards until the magnet 53 enters the linear sliding groove of the upper ring 11, at this time, the magnet 53 separates the magnetic attraction part 13b and the clamping magnetic attraction rod 14 along the chamfered slope, so that the magnetic attraction part 13b moves outward until the connecting part of the limiting part 13a and the magnetic attraction part 13b leaves the linear sliding groove of the upper ring 11, so that the limiting part 13a turns downwards under the action of gravity, and the clamping magnetic attraction rod 14 moves to the side close to the stem of the plant 9, so as to clamp and fix the plant 9; S403: Power on the coil 54, the coil 54 is magnetically connected with the magnet 53; S404: Start the lifting rod 52, the movable end of the lifting rod 52 retreats, the movable end of the lifting rod 52 drives the root support 1 and the plant 9 wound on the root support 1 to move out of the culture pot 4 synchronously, and drives the root support 1 and the plant 9 wound on the root support 1 to shift to the elution box 61; S405: Power on the first electromagnetic plate 64, the first electromagnetic plate 64 generates a magnetic field, and uses the principle of same polarity repulsion to make the lifting plate 62 move upwards until the lifting plate 62 is located at the top of the limiting groove of the culture shelf 3; S406: Open the elution pump 34 fixedly arranged in the elution liquid containing cavity, the elution pump 34 pumps out the elution liquid, the elution liquid enters the inside of the elution box 61 after passing through the L-shaped channel in the lifting plate 62; at this time, the L-shaped connecting part in the lifting plate 62 no longer blocks the liquid outlet hole of the elution box 61, so that the root exudates enter the root exudate collection box 2 with the elution liquid, and the collection of the root exudates is realized; S5: After the collection of the root exudates is completed, start the lifting rod 52, so that the lifting rod 52 drives the root support 1 to return to the culture pot 4 through the magnet 53, and the culture soil is put into the culture pot 4 again; S6: Power off the coil 54, so as to withdraw the magnet 53 from the culture pot 4, so as to continue the soil culture; S7: After a period of time, repeat step S4 to continuously collect the root exudates.

[0060] Taking the water culture of the plant 9 as an example: S1: The experimenter manually rotates the rotating plate 51, so that the rotating plate 51 is located directly above the culture pot 4; S2: Start the lifting rod 52, the lifting rod 52 drives the root support 1 to rise through the magnet 53, and the lifting rod 52 moves the root support 1 into the elution box 61; S3: Open the irrigation pump 35 fixedly arranged in the nutrient solution storage cavity 33, the irrigation pump 35 pumps out the nutrient solution, and the nutrient solution enters the inside of the elution box 61 after passing through the L-shaped channel in the lifting plate 62, so as to water the plant 9; S4: After a period of hydroponics, the root exudates of the hydroponic plant 9 are collected; specifically, the step includes the following steps: S401: The first electromagnetic plate 64 is powered on, the first electromagnetic plate 64 generates a magnetic field, and the principle of same polarity repulsion is used to move the lifting plate 62 upward until the lifting plate 62 is located at the top of the limiting groove of the culture shelf 3; S402: The elution pump 34 fixedly arranged in the elution liquid containing cavity is opened, the elution pump 34 pumps out the elution liquid, and after the elution liquid passes through the L-shaped channel in the lifting plate 62, it enters the inside of the elution box 61; at this time, the L-shaped connecting part in the lifting plate 62 no longer blocks the liquid outlet hole of the elution box 61, so that the root exudates enter the root exudate collection tank 2 with the elution liquid, and the collection of the root exudates is realized; S5: After the collection is completed, the first electromagnetic plate 64 is powered off, the height of the lifting plate 62 is lowered, and the lifting plate 62 returns to the initial position, and then the irrigation pump 35 is opened, the irrigation pump 35 pumps out the nutrient solution, and after the nutrient solution passes through the L-shaped channel in the lifting plate 62, it enters the inside of the elution box 61, so as to hydroponic the plant 9, and then the root exudates can be collected again after a period of hydroponics.

[0061] The contents not described in detail in the specification belong to the prior art known to those skilled in the art.

Claims

1. A multifunctional test device for continuous collection of root exudates, comprising a culture shelf (3) and two culture pots (4), characterized in that, Also include two test mechanism corresponding to the culture pot (4) are connected respectively, each test mechanism includes: Root support (1), provided in the culture pot (4); Root exudate collection assembly, provided on the culture rack (3), and communicated with the eluent containing cavity (31) in the culture rack (3); Root exudate collection box (2), provided on the culture rack (3), and connected with the root exudate collection assembly.

2. The multi-functional test device for continuously collecting root exudates according to claim 1, wherein Root exudate collection assembly includes: Root traction unit (5), rotatably connected with the culture rack (3), and connected with the root support (1); Root elution unit (6), located between the root traction unit (5) and the culture pot (4), and connected with the root exudate collection box (2).

3. A multi-functional test device for continuously collecting root exudates according to claim 2, wherein Root traction unit (5) includes: Rotary plate (51), rotatably connected with the culture rack (3); Lifting rod (52), vertically provided on the bottom surface of the rotary plate (51); Magnet (53), horizontally provided on the bottom of the lifting rod (52) Power coil (54), provided on the upper surface of the root support (1), and in contact with the magnet (53).

4. The multi-functional test apparatus for continuously collecting root exudates according to claim 2, wherein Root elution unit (6) includes: Elution box (61), located above the culture pot (4), and connected with the root exudate collection box (2); Lifting plate (62), provided in the elution box (61), and slidably connected with the elution box (61) Transverse telescopic rod (63), provided inside the root exudate collection box (2), and connected with the elution box (61).

5. A multi-functional test device for continuously collecting root exudates according to claim 4, wherein Root elution unit (6) further includes: First electromagnetic plate (64), provided inside the bottom surface inside the elution box (61); Second magnetic attraction plate (65), provided on the bottom surface of the lifting plate (62), and magnetically connected with the first electromagnetic plate (64).

6. The multi-functional test apparatus for continuously collecting root exudates according to claim 2, wherein Root support (1) includes: Upper collar (11), the upper surface is provided with a groove; Lower collar (12), connected with the upper collar (11) through a plurality of vertical rods (15); Limiting magnetic attraction rod (13), provided in the groove of the upper collar (11); Clamping magnetic attraction rod (14), provided in the groove of the upper collar (11), and magnetically connected with the limiting magnetic attraction rod (13).

7. The multi-functional test apparatus for continuously collecting root exudates according to claim 5, wherein Limiting magnetic attraction rod (13) includes a limiting portion (13a), and a magnetic attraction portion (13b) rotatably connected with the limiting portion (13a); the magnetic attraction portion (13b) is slidably connected with the bottom surface of the groove of the upper collar (11).

8. The multi-functional test device for continuously collecting root exudates according to claim 1, wherein The bottom surface of each culture pot (4) is provided with a soil humidity measuring assembly (7); the soil humidity measuring assembly (7) includes: Load bearing plate (71), the upper surface is connected with the root support (1); Load bearing rod (72), the upper end is connected with the load bearing plate (71), the lower end passes through the culture pot (4), and is located outside the culture pot (4); Measuring spring (73), located in the culture pot (4), and sleeved with the load bearing rod (72).

9. A multi-functional test device for continuously collecting root exudates according to claim 8, wherein The bottom surface of each culture pot (4) is also provided with a soil loosening assembly (8), and the soil loosening assembly (8) includes: Rotary ring (81), rotatably connected with the load bearing plate (71); A plurality of soil loosening rods (82), vertically provided on the upper surface of the rotary ring (81); Rotary handle (83), the upper end is connected with the rotary ring (81), the lower end passes out of the culture pot (4), and is located outside the culture pot (4).

10. The multi-functional test device for continuously collecting root exudates according to claim 1, wherein The culture shelf (3) further comprises a nutrient solution storage cavity (32) and a soil culture watering pipeline (33). One end of the soil culture watering pipeline (33) is communicated with the nutrient solution storage cavity (32), and the other end is communicated with the pipeline arranged in the root system support (1).

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