Liquid transfer device, reaction device, and analysis device

Abstract

The invention discloses a liquid transfer device, a reaction device and an analysis device. Wherein the liquid transfer device is used for guiding the reaction liquid to the reaction surface of the sample carrier. The fixing part comprises a fixing structure which is used for fixing a sample carrier; the first base material comprises a first wall surface, the first base material has a first position and a second position relative to the fixed part, the first wall surface is used for obtaining the reaction liquid when the first base material is in the first position, and the first wall surface is used for guiding the reaction liquid to the reaction surface when the first base material is in the second position; the first driving part is configured to drive the first base material to perform position switching between a first position and a second position; the Mohs hardness of the first base material is greater than or equal to 5H. According to the invention, the distance between the first wall surface and the reaction surface of the sample carrier can be effectively controlled, so that the transfer quality of the reaction liquid is ensured, and the situation that the reaction surface is damaged due to the fact that the first wall surface touches the reaction surface or the reaction liquid cannot be completely transferred to the reaction surface due to overlarge distance between the first wall surface and the reaction surface is avoided.

Description

Liquid transfer devices, reaction devices and analysis devices Technical Field

[0001] The present invention relates to the technical field of biochemical reactions, and in particular to a liquid transfer device, a reaction device and an analysis device. Background Art

[0002] Through gene sequencing, the sequence of target gene fragments can be obtained to facilitate further molecular biology research and gene modification. It can be used to study various diseases and gene expression, and is of great significance in many fields such as biology, medicine and environmental science.

[0003] Currently, mainstream high-throughput sequencing technology is based on the principle of sequencing by synthesis, where DNA nanospheres are formed within the prepared library to amplify fluorescent signals. These nanospheres are then affixed to an arrayed sequencing chip according to a specific pattern and spacing. Through each round of reaction with a specific enzyme and fluorescent probe, different bases emit fluorescent signals of varying wavelengths. These signals are captured by an on-board sequencing imaging system, and the images are reconstructed and identified to sequentially determine the base sequence for each round.

[0004] Existing high-throughput sequencing technologies widely utilize flow cells to perform the sequencing process. These cells perform multiple rounds of imaging on a planar medium within the flow cell to identify bases. However, the biochemical reactions between imaging steps require precise temperature and fluid control, creating demanding environmental requirements. Furthermore, each cycle completely replaces the previous reagent, requiring a large amount of the next reagent to be flushed before the reaction can proceed, resulting in high reagent consumption. The sequencing process requires multiple reagents to undergo up to hundreds of "reaction-photography" cycles, resulting in low sequencing efficiency and high production costs.

[0005] In related technologies, to reduce costs and increase throughput, liquid transfer devices are used to accomplish liquid transfer. Specifically, a thin layer of reagent is coated on the surface of a film substrate, which is then driven toward a sample carrier. The moving film substrate passes through the sample carrier and transfers the reagent layer it carries to the sample carrier, thereby achieving liquid transfer. However, because the film substrate is made of a flexible material, it can vibrate during the process of moving the reagent layer and the film substrate toward the sample carrier, causing it to easily rub against the sample carrier, affecting the stability of the liquid transfer.

[0006] Summary of the Invention

[0007] The main purpose of the present invention is to provide a liquid transfer device, a reaction device and an analysis device, which can effectively control the distance between the first wall surface and the reaction surface of the sample carrier.

[0008] To achieve the above objectives, the present invention provides a liquid transfer device, comprising a fixing portion, a first substrate, and a first driving portion.

[0009] The fixing portion includes a fixing structure, and the fixing structure is used to fix the sample carrier;

[0010] The first substrate includes a first wall surface, the first substrate having a first position and a second position relative to the fixing portion, the first wall surface being used to obtain the reaction liquid when the first substrate is in the first position, and being used to guide the reaction liquid to the reaction surface when the first substrate is in the second position;

[0011] The first driving unit is configured to drive the first substrate to switch between the first position and the second position;

[0012] Wherein, the Mohs hardness of the first substrate is greater than or equal to 5H.

[0013] In some embodiments, the first wall is planar and parallel to the reaction surface, and the first driving unit is configured to drive the first substrate to translate along a direction parallel to the first wall, so that the first substrate switches between the first position and the second position;

[0014] or,

[0015] The first wall surface is planar and parallel to the reaction surface, and the first driving unit is configured to drive the first substrate to rotate around an axis perpendicular to the first wall surface, so that the first substrate switches between a first position and a second position;

[0016] or,

[0017] The first wall surface is a cylindrical surface, the central axis of the cylindrical surface is parallel to the reaction surface, and the first driving part is configured to drive the first substrate to rotate around the central axis so that the first substrate switches between the first position and the second position.

[0018] In some embodiments, the liquid transfer device further includes a liquid distributing unit and a cleaning unit, wherein the liquid distributing unit is configured to distribute the reaction liquid to the first wall surface when the first substrate is in the first position, and the cleaning unit is configured to clean the first wall surface after the first substrate transfers the reaction liquid to the reaction surface at the second position and moves to the first position;

[0019] or,

[0020] The first substrate also has a third position relative to the fixed part. The first driving part is configured to drive the first substrate to switch between the second position and the third position. The liquid transfer device includes a liquid distribution part and a cleaning part. The liquid distribution part is configured to arrange the reaction liquid toward the first wall surface when the first substrate is in the first position. The cleaning part is configured to clean the first wall surface when the first substrate is in the third position.

[0021] In some embodiments, the liquid transfer device further includes a second substrate, the second substrate including a second wall surface, the second substrate having a fourth position and a fifth position relative to the fixing portion, the second wall surface being used to obtain the reaction liquid when the second substrate is in the fourth position, and the second wall surface being used to direct the reaction liquid to the reaction surface when the second substrate is in the fifth position; the Mohs hardness of the second substrate is greater than or equal to 5H;

[0022] The first driving unit is configured to drive the second substrate to switch between the fourth position and the fifth position; or the liquid transfer device further includes a second driving unit, and the second driving unit is configured to drive the second substrate to switch between the fourth position and the fifth position.

[0023] In some embodiments, the first driving unit is configured to drive the second substrate to a fifth position spaced opposite to the fixed structure when the first substrate is in the first position, and to drive the second substrate to a fourth position spaced opposite to the fixed structure when the first substrate is in the second position;

[0024] or,

[0025] The first driving unit is configured to drive the second substrate to the fourth position when the first substrate is in the first position, and to drive the second substrate to the fifth position when the first substrate is in the second position; the liquid transfer device also includes a third driving unit, the third driving unit is connected to the fixing unit, and the third driving unit drives the fixing unit to switch positions between the sixth position and the seventh position. When the fixing unit is in the sixth position, the fixed structure is arranged relative to the first wall surface of the first substrate located in the second position; when it is in the seventh position, the fixed structure is arranged relative to the second wall surface of the second substrate located in the fifth position.

[0026] In some embodiments, the first substrate and the second substrate are arranged opposite to each other along a first direction, and the first driving unit is configured to drive the first substrate and the second substrate to reciprocate along the first direction, so that when the first driving unit drives the first substrate to the first position, the second substrate is driven to the fifth position and opposite to the fixed structure, and when the first driving unit drives the first substrate to the second position and opposite to the fixed structure, the second substrate is driven to the fourth position.

[0027] or,

[0028] The first substrate and the second substrate are arranged relative to each other along the second direction, and the first driving unit is configured to drive the first substrate and the second substrate to reciprocate along the first direction perpendicular to the second direction, so that when the first driving unit drives the first substrate to be in the first position, it drives the second substrate to be in the fourth position, and when the first substrate is in the second position, it drives the second substrate to be in the fifth position; the liquid transfer device also includes a third driving unit, the third driving unit is connected to the fixing unit, and drives the fixing unit to switch positions between the sixth position and the seventh position. When the fixing unit is in the sixth position, the fixing structure is arranged relative to the first wall surface of the first substrate located in the second position, and when it is in the seventh position, the fixing structure is arranged relative to the first wall surface of the second substrate located in the fifth position.

[0029] In some embodiments, the first wall is planar and parallel to the reaction surface, and the first driving unit is configured to drive the first substrate to rotate about an axis perpendicular to the first wall so that the first substrate switches between a first position and a second position; the axis passes through the first wall;

[0030] The first wall surface includes a first area and a second area, and the first area and the second area are distributed circumferentially along the axis. When the first substrate is in the first position, the first area is used to obtain the reaction liquid. When the first substrate is in the second position, the first area is used to transfer the obtained reaction liquid to the reaction surface.

[0031] In some embodiments, the first wall further includes a third region, and the first region, the second region, and the third region are sequentially arranged along the rotation direction of the first substrate;

[0032] The first substrate further includes a third position, and along the rotation direction of the first substrate, the first driving unit drives the first substrate to cyclically rotate in the order of rotating from the first position to the second position, from the second position to the third position, and from the third position to the first position;

[0033] The liquid transfer device includes a liquid distribution part and a cleaning part; when the first substrate is located in the first position, the second position and the third position in turn, the first area, the third area and the second area successively obtain the reaction liquid from the liquid distribution part, the second area, the first area and the third area successively transfer the reaction liquid to the reaction surface, and the third area, the second area and the first area are successively cleaned by the cleaning part.

[0034] In some embodiments, the first wall is planar and parallel to the reaction surface, and the first driving unit is configured to drive the first substrate to rotate about an axis perpendicular to the first wall so that the first substrate switches between a first position and a second position; the axis passes through the first wall;

[0035] The liquid transfer device further includes a second substrate, the second substrate including a second wall surface, the second wall surface being annular and disposed around the outside of the first wall surface, the first drive unit being configured to drive the second wall surface to rotate about an axis so as to switch the second substrate between a fourth position and a fifth position, wherein the second wall surface is used to obtain the reaction liquid when the second substrate is in the fourth position, and the second wall surface transfers the reaction liquid to the reaction surface when the second substrate is in the fifth position; the Mohs hardness of the second substrate is greater than or equal to 5H;

[0036] The liquid transfer device also includes a third driving part, which is connected to the fixed part and can drive the fixed part to switch between a sixth position and a seventh position in a direction perpendicular to the axis. When the fixed part is in the sixth position, the fixed structure is opposite to the first wall surface. When the fixed part is in the seventh position, the fixed structure is opposite to the second wall surface.

[0037] In some embodiments, the first substrate is made of glass or ceramic; or the first wall is plasma / corona modified to increase the surface tension of the first wall; or the distance between the first wall and the reaction surface when in the second position is twice the thickness of the reaction liquid.

[0038] In some embodiments, the liquid transfer device satisfies one of the following conditions a)-f):

[0039] a) The flatness P of the first substrate satisfies: 0um≤P≤3um;

[0040] b) the first wall and the reaction surface are both planar, the first wall is parallel to the reaction surface, the first substrate is capable of rotating about an axis perpendicular to the first wall, and the area of ​​the first wall is not less than twice the area of ​​the reaction surface;

[0041] c) the first driving unit drives the first substrate to move along the third direction to the second position, wherein a cross section of the first substrate has a first cross section side parallel to the third direction along a direction parallel to the first wall surface, and a length of the first cross section side along the third direction is greater than twice a length of the reaction surface;

[0042] d) the first driving unit drives the first substrate to move along the third direction to a second position, wherein a cross section of the first substrate has a second cross section side perpendicular to the third direction along a direction parallel to the first wall surface, and a length of the second cross section side perpendicular to the third direction is greater than a length of the reaction surface;

[0043] e) The liquid transfer device further includes a liquid distribution unit and a cleaning unit, wherein the liquid distribution unit is configured to distribute the reaction liquid to the first wall surface when the first substrate is in the first position, and the cleaning unit is configured to clean the first wall surface after the first substrate transfers the reaction liquid to the reaction surface in the second position;

[0044] The first driving unit is configured to drive the first substrate to move along a first direction, and the cleaning unit, the liquid distributing unit, the first substrate and the fixing unit are arranged relative to each other along the first direction.

[0045] and / or,

[0046] The first driving portion is configured to drive the first substrate to rotate around an axis perpendicular to the first wall surface, and the cleaning portion, the liquid distributing portion, and the fixing portion are distributed along the circumference of the axis;

[0047] f), the first driving unit is configured to drive the first substrate to move in a first direction, the liquid transfer device further comprising a liquid distributing unit, the liquid distributing unit being configured to distribute the reaction liquid toward the first wall surface when the first substrate is in the first position, and the cleaning unit being configured to clean the first wall surface after the first substrate transfers the reaction liquid to the reaction surface in the second position;

[0048] When the first substrate is in the first position, the first substrate and the liquid distributing part are arranged opposite to each other along a second direction perpendicular to the first direction. When the first substrate is in the second position, the first substrate and the fixing part are arranged opposite to each other along the second direction.

[0049] An embodiment of the second aspect of the present invention further provides a liquid transfer device for directing a reaction liquid to a reaction surface of a sample carrier. The liquid transfer device includes a fixing portion, a first substrate, and a first driving portion.

[0050] The fixing portion includes a fixing structure for fixing the sample carrier, and the fixing portion has a first position and a second position relative to the fixing portion;

[0051] The first substrate includes a first wall surface, the first wall surface is used to obtain the reaction liquid when the fixing portion is in the first position, and the first wall surface is used to guide the reaction liquid to the reaction surface when the fixing portion is in the second position;

[0052] The first driving portion is configured to drive the fixing portion to switch between the first position and the second position;

[0053] Wherein, the Mohs hardness of the first substrate is greater than or equal to 5H.

[0054] An embodiment of the third aspect of the present invention further provides a reaction device, comprising the liquid transfer device and a sample carrier according to any of the above embodiments.

[0055] An embodiment of the fourth aspect of the present invention further provides an analysis device, comprising the reaction device of any of the above embodiments.

[0056] Compared with the prior art, the present invention has the following beneficial effects:

[0057] In the technical solution of the present invention, a liquid transfer device includes a fixing portion, a first substrate, and a first driving portion. The fixing portion includes a fixing structure for fixing a sample carrier. The first substrate includes a first wall surface, which has a first position and a second position relative to the fixing portion. When the first substrate is in the first position, the first wall surface is used to receive the reaction liquid, and when the first substrate is in the second position, the first wall surface is used to direct the reaction liquid to a reaction surface. The first driving portion is configured to drive the first substrate to switch between the first and second positions. In particular, in the present invention, the Mohs hardness of the first substrate is greater than or equal to 5H. Compared to substrates made of flexible film materials, when the Mohs hardness of the first substrate is greater than or equal to 5H, the first wall surface is less likely to vibrate during the transfer of the reaction liquid to the reaction surface, thereby effectively controlling the distance between the first wall surface and the reaction surface of the sample carrier, thereby ensuring the quality of the reaction liquid transfer and preventing the first wall surface from contacting the reaction surface and damaging the reaction surface, or preventing the first wall surface from contacting the reaction surface and causing incomplete transfer of the reaction liquid to the reaction surface due to excessive distance between the first wall surface and the reaction surface. BRIEF DESCRIPTION OF THE DRAWINGS

[0058] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without paying any creative work.

[0059] FIG1 is a side view schematically illustrating a liquid transfer device provided in a first embodiment of the present invention, wherein the fixing portion and the first driving portion are removed, and the first substrate is located in a first position;

[0060] FIG2 is a side view of a liquid transfer device according to a first embodiment of the present invention, wherein the fixing portion and the first driving portion are removed, and the first substrate is located in the second position;

[0061] FIG3 is a perspective schematic diagram of a liquid transfer device provided in a second embodiment of the present invention;

[0062] FIG4 is a side view schematic diagram of a liquid transfer device provided in a third embodiment of the present invention;

[0063] FIG5 is a perspective schematic diagram of a liquid transfer device provided in a fourth embodiment of the present invention;

[0064] FIG6 is a first side schematic view of a liquid transfer device provided in a fifth embodiment of the present invention;

[0065] FIG7 is a second side view of the liquid transfer device provided in the fifth embodiment of the present invention, wherein the fixing portion and the first driving portion are removed;

[0066] FIG8 is a first side schematic view of a liquid transfer device provided in a sixth embodiment of the present invention;

[0067] FIG9 is a second side view of the liquid transfer device provided in the sixth embodiment of the present invention, wherein the fixing portion and the first driving portion are removed;

[0068] FIG10 is a side schematic view of a liquid transfer device provided in a seventh embodiment of the present invention;

[0069] FIG11 is a side view of a liquid transfer device according to an eighth embodiment of the present invention, wherein the fixing portion and the first driving portion are removed, the first substrate is located at the first position, and the second substrate is located at the fifth position;

[0070] FIG12 is a side view of a liquid transfer device according to an eighth embodiment of the present invention, wherein the fixing portion and the first driving portion are removed, the first substrate is located in the second position, and the second substrate is located in the fourth position;

[0071] FIG13 is a side view of a liquid transfer device provided in a ninth embodiment of the present invention, wherein the fixing portion and the first driving portion are removed, the first substrate is located in the first position, and the second substrate is located in the fourth position;

[0072] FIG14 is a side view of a liquid transfer device provided in a ninth embodiment of the present invention, wherein the fixing portion and the first driving portion are removed, the first substrate is located in the second position, and the second substrate is located in the fifth position;

[0073] FIG15 is a side view of a liquid transfer device according to a tenth embodiment of the present invention, wherein the fixing portion and the first driving portion are removed, the first substrate is in the first position, the second substrate is in the fourth position, and the fixing portion is in the seventh position;

[0074] FIG16 is a side view of a liquid transfer device according to a tenth embodiment of the present invention, wherein the fixing portion and the first driving portion are removed, the first substrate is in the second position, the second substrate is in the fifth position, and the fixing portion is in the sixth position;

[0075] FIG17 is a side view of a liquid transfer device according to a tenth embodiment of the present invention, wherein the fixing portion and the first driving portion are removed, the first substrate is in the second position, the second substrate is in the fifth position, and the fixing portion is in the sixth position;

[0076] FIG18 is a side view of a liquid transfer device according to an eleventh embodiment of the present invention, wherein the fixing portion and the first driving portion are removed, the first substrate is located at the first position, and the second substrate is located at the fifth position;

[0077] FIG19 is a side view of a liquid transfer device according to an eleventh embodiment of the present invention, wherein the fixing portion and the first driving portion are removed, the first substrate is located in the second position, and the second substrate is located in the fourth position;

[0078] FIG20 is a side view of a liquid transfer device according to a twelfth embodiment of the present invention; wherein the first substrate is located at the second position and the second substrate is located at the fifth position;

[0079] FIG21 is a side view of a liquid transfer device according to a thirteenth embodiment of the present invention; wherein the first substrate is located at the second position, and the second substrate is located at the fifth position;

[0080] FIG22 is a side view of a liquid transfer device according to a fourteenth embodiment of the present invention, wherein the fixing portion and the first driving portion are removed, and the first substrate is located in the first position;

[0081] FIG23 is a side view of a liquid transfer device according to a fourteenth embodiment of the present invention, wherein the fixing portion and the first driving portion are removed, and the first substrate is located in the second position;

[0082] FIG24 is a side view of a liquid transfer device provided in a fifteenth embodiment of the present invention, wherein the fixing portion and the first driving portion are removed, and the first substrate is located in the first position;

[0083] FIG25 is a side view of a liquid transfer device provided in a fifteenth embodiment of the present invention, wherein the fixing portion and the first driving portion are removed, and the first substrate is located in the second position;

[0084] FIG26 is a side view of a liquid transfer device provided in a sixteenth embodiment of the present invention, wherein the fixing portion and the first driving portion are removed, and the first substrate is located in the first position;

[0085] FIG27 is a side view of a liquid transfer device provided in a sixteenth embodiment of the present invention, wherein the fixing portion and the first driving portion are removed, and the first substrate is located in the second position;

[0086] FIG28 is a side view of a liquid transfer device provided in a sixteenth embodiment of the present invention, wherein the fixing portion and the first driving portion are removed, and the first substrate is located at the third position;

[0087] FIG29 is a side view of a liquid transfer device according to a seventeenth embodiment of the present invention, wherein the fixing portion and the first driving portion are removed, the first substrate is in the second position, the second substrate is in the fourth position, and the fixing portion is in the sixth position;

[0088] FIG30 is a side view of a liquid transfer device according to a seventeenth embodiment of the present invention, wherein the fixing portion and the first driving portion are removed, the first substrate is in the first position, the second substrate is in the fifth position, and the fixing portion is in the seventh position;

[0089] FIG31 is a side view of a liquid transfer device according to an eighteenth embodiment of the present invention, wherein the fixing portion and the first driving portion are removed, the first substrate is in the second position, the second substrate is in the fourth position, and the fixing portion is in the sixth position;

[0090] FIG32 is a side view of a liquid transfer device provided in an embodiment of the second aspect of the present invention, wherein the fixing portion and the first driving portion are removed, and the fixing portion is located in the second position;

[0091] Figure 33 is a side schematic diagram of the liquid transfer device provided in an embodiment of the second aspect of the present invention; wherein the fixing portion and the first driving portion are removed, and the fixing portion is located in the first position.

[0092] DESCRIPTION OF REFERENCE NUMERALS: 100 - liquid transfer device; 110 - fixing portion; 111 - fixing structure; 112 - sixth position; 113 - seventh position; 120 - first substrate; 121 - first wall; 1211 - first region; 1212 - second region; 1213 - third region; 122 - first position; 123 - second position; 124 - third position; 125 - side edge of first cross section; 126 - side edge of second cross section; 130 - first driving portion; 140 - liquid dispensing portion; 150 - cleaning portion; 160 - second substrate; 161 - second wall; 162 - fourth position; 163 - fifth position; 170 - third driving portion; 200 - sample carrier; 210 - reaction surface; 300 - liquid transfer device; 310 - first substrate; 311 - first position; 312 - second position; X1 - first direction; Y - second direction; X2-third direction.

[0093] The purpose, features and advantages of the present invention will be further described with reference to the accompanying drawings and in conjunction with the embodiments. DETAILED DESCRIPTION

[0094] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making any creative efforts shall fall within the scope of protection of the present invention.

[0095] It should be noted that if the embodiments of the present invention involve directional indications (such as up, down, left, right, front, back, etc.), the directional indications are only used to explain the positional relationship, movement status, etc. between the components under a certain specific posture. If the specific posture changes, the directional indications will also change accordingly.

[0096] In addition, if there are descriptions involving "first", "second", etc. in the embodiments of the present invention, the descriptions of "first", "second", etc. are only for descriptive purposes and cannot be understood as indicating or suggesting their relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features limited to "first" and "second" may explicitly or implicitly include at least one of such features. In addition, if "and / or", "and / or" or "and / or" appear in the full text, its meaning includes three parallel schemes. Taking "A and / or B" as an example, it includes scheme A, or scheme B, or a scheme in which A and B are satisfied at the same time. In addition, the technical solutions between the various embodiments can be combined with each other, but it must be based on the ability of ordinary technicians in this field to implement it. When the combination of technical solutions is mutually contradictory or cannot be implemented, it should be deemed that such a combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0097] In related technologies, to reduce costs and increase throughput, liquid transfer devices are used to accomplish liquid transfer. Specifically, a thin layer of reagent is coated on the surface of a film substrate, which is then driven toward a sample carrier. The moving film substrate passes through the sample carrier and transfers the reagent layer it carries to the sample carrier, thereby achieving liquid transfer. However, because the film substrate is made of a flexible material, it can vibrate during the process of moving the reagent layer with it toward the sample carrier, causing it to easily rub against the sample carrier, affecting the stability of the liquid transfer.

[0098] In view of this, referring to Figures 1 to 31, an embodiment of the first aspect of the present invention provides a liquid transfer device 100 for directing a reaction liquid to a reaction surface 210 of a sample carrier 200. Specifically, in some embodiments, the reaction liquid in the present application may be a liquid reagent, a thin layer of reagent (liquid film), etc., and the sample carrier 200 may be a chip, an imageable substance, or a biological material, etc. The function of the liquid transfer device 100 in the present application can be understood as enabling the reaction liquid to be transferred to the reaction surface 210 of the sample carrier 200. Therefore, in some embodiments, the liquid transfer device 100 may only need to position the reaction liquid around the reaction surface 210, and the reaction liquid can be directed to the reaction surface 210 through the properties of the reaction liquid or other physical / chemical phenomena. At the same time, the working process of the liquid transfer device 100 may include multiple processes of taking liquid, moving, and guiding liquid. Specifically, the liquid transfer device 100 includes a fixing portion 110, a first substrate 120, and a first driving portion 130.

[0099] 1 to 3 , the fixing portion 110 includes a fixing structure 111. The fixing structure 111 is used to fix the sample carrier 200. That is, the fixing structure 111 is used to fix and place the sample carrier 200. Specifically, in some embodiments, the fixing portion 110 may include a support plate, and the support plate is used to support and place the sample carrier 200. In order to better fix the sample carrier 200, in some embodiments, on the one hand, the fixing structure 111 may be a clamping member, and the clamping member may include a clamping claw, a clamping block, etc.; on the other hand, the fixing structure 111 may be a connecting fixing structure such as a snap-on member, a magnetic member, etc.; on the other hand, the fixing structure 111 may have a placement cavity, so that the sample carrier 200 is suitable for being placed in the placement cavity.

[0100] Referring to Figures 1-3, the first substrate 120 includes a first wall 121. The first substrate 120 has a first position 122 and a second position 123 relative to the fixed portion 110. When the first substrate 120 is in the first position 122, the first wall 121 is used to receive the reaction liquid. Depending on the type of reaction liquid and the method of liquid delivery, in some embodiments, the reaction liquid can be placed in a reagent spray head, a reagent tank, or a reagent tube. It will be understood that when the first substrate 120 is in the first position 122, the first wall 121 can receive the reaction liquid. Specifically, in some embodiments, the first wall 121 can receive the reaction liquid by relative motion between the first wall 121 and the reaction liquid, generating shear force within a suitable gap, thereby transferring the reaction liquid to the first wall 121; or the reaction liquid can be poured or injected onto the first wall 121 by an external force. Furthermore, when the first substrate 120 is in the second position 123, the first wall 121 is used to direct the reaction liquid to the reaction surface 210. It will be appreciated that when the first substrate 120 is in the second position 123, the first wall 121 can direct the reaction liquid thereon to the reaction surface 210 of the sample carrier 200. Specifically, in some embodiments, the first wall 121 can direct the reaction liquid to the reaction surface 210 by causing the first substrate 120 and the sample carrier 200 to move relative to each other within a suitable gap range, thereby generating a shear force that can transfer the reaction liquid from the first substrate 120 to the reaction surface 210 of the sample carrier 200. More specifically, in some embodiments, the suitable gap range can be a gap that satisfies a minimum reaction liquid replacement ratio.

[0101] 1-3 , the first drive unit 130 is configured to drive the first substrate 120 to switch positions between a first position 122 and a second position 123. It will be appreciated that the first drive unit 130 is used to drive the first substrate 120 to move to the first position 122 or the second position 123. Based on the function of the first drive unit 130, in some embodiments, the first drive unit 130 may include a drive device such as a motor, a hydraulic cylinder, a pneumatic cylinder, or an electric cylinder, thereby facilitating control of the driving action of the first drive unit 130. The first drive unit 130 may also include a mechanical actuation structure, such as a rotating shaft structure or a telescopic structure, thereby enabling the first drive unit 130 to be driven solely by human power.

[0102] When the first substrate 120 is in the second position 123 and directing the reaction liquid toward the reaction surface 210, any vibration of the first substrate 120 or the fixing portion 110 due to external or internal factors may affect the stable control of the gap between the first substrate 120 and the reaction surface 210. Therefore, to improve the stability of the reaction liquid transfer, in the present invention, the first substrate 120 has a Mohs hardness of 5H or greater. The Mohs hardness scale is a measurement standard for hardness. It involves scratching the surface of the object being tested and measuring the depth of the scratch, which is used to represent the Mohs hardness. The hardness of the object being tested is determined by comparing the scratches made with a standard mineral in the Mohs hardness scale.

[0103] Based on the combination of the above embodiments, it can be seen that the liquid transfer device 100 includes a fixing portion 110, a first substrate 120, and a first driving portion 130. The fixing portion 110 includes a fixing structure 111, which is used to fix the sample carrier 200. The first substrate 120 includes a first wall 121, and the first substrate 120 has a first position 122 and a second position 123 relative to the fixing portion 110. When the first substrate 120 is in the first position 122, the first wall 121 is used to obtain the reaction liquid. When the first substrate 120 is in the second position 123, the first wall 121 is used to guide the reaction liquid to the reaction surface 210. The first driving portion 130 is configured to drive the first substrate 120 to switch between the first position 122 and the second position 123. In particular, in the present invention, the Mohs hardness of the first substrate 120 is greater than or equal to 5H. Compared with a substrate made of a flexible film material, when the Mohs hardness of the first substrate 120 is greater than or equal to 5H, the first wall 121 is less likely to vibrate during the process of transferring the reaction liquid to the reaction surface 210; at the same time, when the Mohs hardness of the first substrate 120 is greater than or equal to 5H, the transfer effect of the reaction liquid is significantly improved compared with the traditional solution. Specifically, see Table 1. From some experimental values, it can be seen that when a flow cell is used to transfer the reaction liquid, the gap / reaction surface reagent thickness needs to be 60μm, and the reagent dosage is 588ul. When a reaction surface is immersed in a When transferring the reaction liquid by the method of the present invention, the gap / reaction surface reagent thickness needs to be 30μm, and the reagent amount is 294ul. When transferring the reaction liquid by the PET film substrate, the gap / reaction surface reagent thickness needs to be 30μm, and the reagent amount is 294ul. When transferring the reaction liquid by the glass substrate (Mohs hardness greater than or equal to 5H), the gap / reaction surface reagent thickness needs to be 10μm, and the reagent amount is 98ul. It can be seen that when the Mohs hardness of the first substrate 120 is greater than or equal to 5H, it is easy to control the pipetting gap and achieve a lower reagent usage. Therefore, the present invention can effectively control the distance between the first wall 121 and the reaction surface 210 of the sample carrier 200, thereby ensuring the transfer quality of the reaction liquid, avoiding the first wall 121 touching the reaction surface 210 and causing damage to the reaction surface 210, or the distance between the first wall 121 and the reaction surface 210 is too large, so that the reaction liquid cannot be completely transferred to the reaction surface 210.

[0104] Table 1

[0105] Corresponding to different liquid guiding requirements, the first substrate 120 can have different shapes or movement forms. Referring to Figures 1-2 and 6, in the first configuration of the first substrate 120, the first wall 121 can be flat and parallel to the reaction surface 210. Based on this, the first substrate 120 can be a plate, and the first wall 121 can be flat. In this embodiment, because the first wall 121 is parallel to the reaction surface 210, the reaction liquid can be more easily guided to the reaction surface 210 under the action of shear force, and the parallel arrangement makes the corresponding spacing between the two surfaces the same, so that the reaction liquid obtained from each part of the reaction surface 210 is more uniform. Specifically, referring to Figures 1-2 and 6, in some embodiments, the first driving unit 130 can be configured to drive the first substrate 120 to translate in a direction parallel to the first wall 121, so that the first substrate 120 switches positions between the first position 122 and the second position 123. It can be understood that in this embodiment, the first position 122 can be arranged relative to the second position 123 in a direction parallel to the first wall 121, so that the first driving unit 130 drives the first substrate 120 to move translationally in a direction parallel to the first wall 121, so that the first substrate 120 can switch between the first position 122 and the second position 123.

[0106] Accordingly, referring to FIG3 , in other embodiments, the first substrate 120 and the sample carrier 200 may be arranged relative to each other along a first direction X1, and the first driving unit 130 may be configured to drive the first substrate 120 to translate in a direction perpendicular to the first direction X1; or the first driving unit 130 may be configured to drive the first substrate 120 to translate in a direction perpendicular to the first wall 121. In this way, the first substrate 120 can be switched between the first position 122 and the second position 123. Regarding the above-mentioned movement mode, in some specific embodiments, see Figure 3, in the initial state, the first wall 121 is located on the upper side wall of the first substrate 120, and the reaction surface 210 is located on the lower side wall of the sample carrier 200. The first substrate 120 can be located at the first position 122 below and obtain the reaction liquid; thereafter, the first driving unit 130 can drive the first substrate 120 to translate along the second direction Y (vertical direction) perpendicular to the first direction X1 to the second position 123, and at the same time, the sample carrier 200 can translate along the first direction X1 (horizontal direction) and approach the first substrate 120 to facilitate obtaining the reaction liquid; after the movement is completed, the first substrate 120 is located at the second position 123, the sample carrier 200 is located below the first substrate 120, and the first wall 121 is opposite to the reaction surface 210, so that the first wall 121 can guide the reaction liquid to the reaction surface 210.

[0107] Referring to Figure 4 , in a second configuration of the first substrate 120, the first wall 121 can be planar and parallel to the reaction surface 210. The first drive unit 130 can be configured to drive the first substrate 120 to rotate about an axis perpendicular to the first wall 121, thereby switching the first substrate 120 between a first position 122 and a second position 123. In this regard, the axis of rotation of the first substrate 120 can be an axis inherent to the first substrate 120 or an axis constructed external to the first substrate 120. Specifically, referring to Figure 4 , in some embodiments, the first substrate 120 can be a flat plate; in other embodiments, the first substrate 120 can be disc-shaped. The first position 122 and the second position 123 can be areas arranged around the rotation axis of the first substrate 120. It will be understood that in this embodiment, the first substrate 120 can rotate about an axis perpendicular to the first wall 121, allowing the first substrate 120 to rotate to circumferentially opposed first and second positions 122 and 123, thereby switching the first substrate 120.

[0108] Referring to Figure 5, in the third configuration of the first substrate 120, the first wall surface 121 can be a cylindrical surface. The central axis of the cylindrical surface can be parallel to the reaction surface 210. Specifically, the first substrate 120 can be cylindrical, based on which, the first wall surface 121 can be the outer peripheral annular wall surface of the first substrate 120. The first driving portion 130 can be configured to drive the first substrate 120 to rotate around the central axis so that the first substrate 120 switches its position between the first position 122 and the second position 123. It can be understood that in this embodiment, during the rolling of the first substrate 120 around the central axis, relative to the position of the reaction surface 210, the first position 122 and the second position 123 of the first substrate 120 along the circumferential direction of the central axis can be respectively opposite to the position of the reaction surface 210.

[0109] In order to make it easier for the first substrate 120 to obtain the reaction liquid. Referring to Figures 6 to 10, in some embodiments, the liquid transfer device 100 may further include a liquid distribution unit 140. The liquid distribution unit 140 may be configured to arrange the reaction liquid toward the first wall 121 when the first substrate 120 is in the first position 122. Specifically, in some embodiments, the liquid distribution unit 140 may include a nozzle to spray the reaction liquid on the first wall 121. The liquid distribution unit 140 may include a reagent holding chamber. After the reaction liquid is injected into the reagent holding chamber, the first substrate 120 can obtain the reaction liquid through the movement of the liquid distribution unit 140 or the movement of the first substrate 120; in some embodiments, the liquid distribution unit 140 may include a reagent tube, and the reagent tube can inject the reaction liquid into the first wall 121.

[0110] In addition, in order to facilitate the cleaning of the first substrate 120. Referring to Figures 6-10, in some embodiments, the liquid transfer device 100 may further include a cleaning unit 150. With regard to the different cleaning forms of the cleaning unit 150, in some embodiments, the cleaning unit 150 can be used to clean the first wall 121; specifically, referring to Figures 6-7, in some embodiments, the cleaning unit 150 can be configured to clean the first wall 121 after the first substrate 120 transfers the reaction liquid to the reaction surface 210 at the second position 123 and moves to the first position 122. It can be understood that in this embodiment, the cleaning unit 150 is located at the first position 122. After the first substrate 120 guides the reaction liquid to the reaction surface 210 at the second position 123 (after the first substrate 120 completes the liquid guiding action), the first substrate 120 can return to the first position 122, and there may be residual reaction liquid on the first wall 121. At this time, the cleaning unit 150 can clean the first wall 121.

[0111] Alternatively, referring to Figures 8-9, in some other embodiments of cleaning forms, the first substrate 120 may further have a third position 124 relative to the fixing portion 110. The first driving portion 130 may be configured to drive the first substrate 120 to switch between the second position 123 and the third position 124. Similarly, the liquid transfer device 100 may include a liquid distribution portion 140 and a cleaning portion 150. The liquid distribution portion 140 may be configured to arrange the reaction liquid toward the first wall surface 121 when the first substrate 120 is in the first position 122. The cleaning portion 150 may be configured to clean the first wall surface 121 when the first substrate 120 is in the third position 124. It can be understood that, similar to the previous embodiment, in this embodiment, the liquid transfer device 100 can also include a liquid distribution part 140 for arranging the reaction liquid and a cleaning part 150 for cleaning the first wall 121. The difference is that the first substrate 120 of this embodiment also has a third position 124 relative to the fixed part 110. The third position 124 can be independently set outside the first position 122 and the second position 123, so that the three positions set by the first substrate 120 can correspond to different operations respectively, which is beneficial to the organization and control of the liquid transfer device 100, and the actions corresponding to each position will not interfere with each other.

[0112] 9-10 , in different embodiments, the relative positional relationship among the first position 122 , the second position 123 , and the third position 124 may vary as the need for liquid conduction changes.

[0113] Regarding the cleaning method of the cleaning unit 150 described above, in some embodiments, the cleaning unit 150 can use a cleaning liquid as the cleaning material. The cleaning liquid can be water, a specially prepared solution, or the like. The cleaning unit 150 can use a nozzle or a needle nozzle to output the cleaning liquid to flush the first substrate 120. In other embodiments, the cleaning unit 150 can include a cleaning liquid holding chamber. After the cleaning liquid is injected into the cleaning liquid holding chamber, the movement of the cleaning unit 150 or the movement of the first substrate 120 allows the first substrate 120 to contact the cleaning liquid, thereby achieving a cleaning effect. In other embodiments, the cleaning unit 150 can use a cleaning tool to clean the first substrate 120. The cleaning tool can include a brush or a cleaning cloth. In other embodiments, a removable and replaceable liquid-conducting membrane can be arranged on the first wall 121. The cleaning unit 150 can achieve a cleaning effect by replacing the liquid-conducting membrane.

[0114] Furthermore, in some embodiments, the cleaning unit 150 may further include a hot air device. After the cleaning process of the cleaning unit 150 is completed, the hot air device may blow hot air to dry the first substrate 120, thereby improving the surface quality of the cleaned first substrate 120 and facilitating the maintenance of the first substrate 120.

[0115] It will be appreciated that the first substrate 120 of the above-described embodiments can be used for operations such as liquid collection, liquid routing, and cleaning when in different positions. Furthermore, to improve the operating efficiency of the liquid transfer device 100, referring to Figures 11-12 and 20, in some embodiments, the liquid transfer device 100 may further include a second substrate 160. The second substrate 160 may include a second wall 161. The second substrate 160 may have a fourth position 162 and a fifth position 163 relative to the fixing portion 110. When the second substrate 160 is in the fourth position 162, the second wall 161 can be used to collect the reaction liquid. When the second substrate 160 is in the fifth position 163, the second wall 161 can be used to direct the reaction liquid to the reaction surface 210. It will be appreciated that in this embodiment, the function of the second substrate 160 is similar to that of the first substrate 120 in the above-described embodiments, and can also be used to collect the reaction liquid or direct the reaction liquid to the reaction surface 210. Therefore, the structure and arrangement of the second substrate 160 can refer to the description of the first substrate 120 in the above-described embodiments. In this embodiment, the second substrate 160 is provided so that the first substrate 120 and the second substrate 160, which have similar functions, can be arranged simultaneously. This allows one substrate to perform operations such as liquid collection, liquid transfer, and cleaning while the other substrate can simultaneously perform another operation. This arrangement can thus improve the efficiency of liquid transfer between multiple sample carriers 200 in the liquid transfer device 100. Furthermore, when a sample carrier 200 requires multiple operations, the two substrates can alternately perform liquid transfer operations on the same sample carrier 200.

[0116] Therefore, in some embodiments, the second substrate 160 can be configured similarly to the first substrate 120. Similarly, to improve the stability of reaction solution transfer, in some embodiments, the Mohs hardness of the second substrate 160 can be greater than or equal to 5H. Similar to the first substrate 120, in this embodiment, limiting the Mohs hardness of the second substrate 160 can effectively control the distance between the second wall 161 and the reaction surface 210 of the sample carrier 200, thereby ensuring the quality of reaction solution transfer and preventing the second wall 161 from contacting the reaction surface 210 and causing damage to the reaction surface 210, or preventing the reaction solution from being completely transferred to the reaction surface 210 due to an excessive distance between the second wall 161 and the reaction surface 210.

[0117] To facilitate switching of the second substrate 160 between different positions, referring to Figures 11-12 and 20, in some embodiments, the first driving unit 130 can be configured to drive the second substrate 160 to switch between the fourth position 162 and the fifth position 163. Alternatively, in other embodiments, the liquid transfer device 100 can further include a second driving unit configured to drive the second substrate 160 to switch between the fourth position 162 and the fifth position 163. In other words, depending on driving requirements, in different embodiments, the first substrate 120 or the second substrate 160 can be driven by the same driving unit, and both can be driven simultaneously; or the two substrates can be driven separately by different driving units.

[0118] At the same time, in order to further improve the working efficiency of the liquid transfer device 100. In some embodiments, the liquid transfer device 100 may further include two or more substrates. For example, the liquid transfer device 100 may further include a third substrate, a fourth substrate, etc. that are equivalent to the first substrate 120 and the second substrate 160. For ease of description, the following embodiment is described in which the liquid transfer device 100 includes the first substrate 120 and the second substrate 160, and the first driving unit 130 is capable of driving the first substrate 120 or the second substrate 160. Different embodiments can be combined with different technical solutions.

[0119] It should be noted that for embodiments in which the liquid transfer device 100 includes multiple substrates, different arrangements may be employed. For example, each substrate may be driven by the first drive unit 130 in the same direction or in different directions. In some embodiments, each substrate may be connected or disconnected from each other, or in other embodiments, each substrate may be a different portion divided from the same base. For ease of description, the following uses an embodiment in which the substrates are disconnected from each other as an example, and different embodiments may be combined with different technical solutions.

[0120] It is understood that both the second substrate 160 and the first substrate 120 can be used for liquid transfer. Furthermore, to achieve efficient cleaning of the reaction surface 210, in some embodiments, both the first substrate 120 and the second substrate 160 can be used to clean the reaction surface 210. Specifically, while one substrate is collecting the reaction liquid, the other can simultaneously clean the reaction surface 210, achieving higher efficiency. In other embodiments, multiple substrates can be provided for cleaning the reaction surface 210. Specifically, in some embodiments, the liquid transfer device 100 can also include a third substrate. Similarly, the third substrate can include a third wall. The third substrate can have a cleaning preparation position and a cleaning position. When the third substrate is in the cleaning preparation position, the third wall is used to collect the cleaning material. When the third substrate is in the cleaning position, the third wall is used to direct the cleaning liquid to the reaction surface 210. The third substrate can also be driven by the first drive unit 130 or another drive unit. In other words, in different embodiments, the cleaning of the reaction surface 210 can be performed alternately by the first substrate 120 and the second substrate 160, or by a separate third substrate.

[0121] Based on the embodiment described above, the liquid transfer device 100 includes a first substrate 120 and a second substrate 160. To enable one substrate to perform preparatory operations such as liquid extraction and cleaning while the other substrate performs liquid transfer operations, the first substrate 120, the second substrate 160, and the first driving unit 130 can be arranged in different ways to enable the liquid transfer device 100 to form different motion modes.

[0122] Specifically, regarding an embodiment in which the first drive unit 130 is capable of driving the first substrate 120 and the second substrate 160 to move parallel to each other, see Figures 11-12 and 20. In the embodiment of the first motion mode, the first drive unit 130 can be configured to drive the second substrate 160 to a fifth position 163 spaced apart from the fixed structure 111 when the first substrate 120 is in the first position 122, and to drive the second substrate 160 to a fourth position 162 spaced apart from the fixed structure 111 when the first substrate 120 is in the second position 123. It will be appreciated that when the first substrate 120 is in the second position 123, the first substrate 120 can direct liquid toward the reaction surface 210, while the second substrate 160 is in the fourth position 162 and can receive the reaction liquid. Conversely, when the first substrate 120 is in the first position 122, the first substrate 120 can receive the reaction liquid, while the second substrate 160 is in the fifth position 163 and can direct liquid toward the reaction surface 210. In this embodiment, the first drive unit 130 can be configured to simultaneously drive the first substrate 120 and the second substrate 160 to move, allowing both substrates to switch between their corresponding positions simultaneously. This arrangement, through the positional switching of the first substrate 120 and the second substrate 160, ensures that at least one substrate can always receive the reaction solution and at least one substrate can always transfer the solution, thereby improving the efficiency of liquid transfer.

[0123] It should be noted that, for embodiments in which the liquid transfer device 100 includes a first substrate 120 and a second substrate 160, in different embodiments, the relative positional relationships between the first position 122, the second position 123, the fourth position 162, and the fifth position 163 can be changed as needed. Depending on the positions of the first substrate 120 and the second substrate 160, the fixing portion 110 can be connected to a plurality of sample carriers 200 located at different positions, or the fixing portion 110 can be connected to a corresponding driving portion to drive the fixing portion 110 to move to different positions. In addition, for embodiments in which the fixing portion 110 needs to change position according to the position of the substrates, the movement direction of the fixing portion 110 can be changed accordingly according to the different arrangements of the first substrate 120 and the second substrate 160.

[0124] In an embodiment of the second motion mode, the first driving unit 130 can be configured to drive the second substrate 160 to the fourth position 162 when the first substrate 120 is in the first position 122, and to drive the second substrate 160 to the fifth position 163 when the first substrate 120 is in the second position 123. It will be appreciated that, referring to Figures 13-14 and 20, in one embodiment, the first substrate 120 and the second substrate 160 can be simultaneously in the liquid extraction position, or the first substrate 120 and the second substrate 160 can be simultaneously in the liquid delivery position, so that the first substrate 120 and the second substrate 160 can work together to improve efficiency.

[0125] In addition, the liquid transfer device 100 may further include a finishing section, which may be used to finish the substrate after the liquid conduction is completed in preparation for the next reaction liquid-conducting operation. Therefore, similar to the above-mentioned cleaning section 150, the finishing section may be used to clean the substrate after the liquid conduction is completed, for example, it may be used to flush and clean the first wall 121 or the second wall 161 after the liquid conduction. And the form of the finishing section may refer to the above description of the cleaning section 150. Further, in some embodiments, the liquid transfer device 100 may further include a third drive section 170, and the third drive section 170 may be connected to the fixed section 110. And the third drive section 170 may drive the fixed section 110 to switch positions between the sixth position 112 and the seventh position 113. It will be understood that in this embodiment, the third drive section 170 may be used to drive the fixed section 110 to adapt to different movement modes of the substrate.

[0126] 15 to 17 and 20 , based on the above configuration, in another case, referring to FIG. 15 , when the first substrate 120 is in the first position 122 and the second substrate 160 is in the fourth position 162, both the first substrate 120 and the second substrate 160 can obtain the reaction liquid; thereafter, referring to FIG. 16 , when the first substrate 120 is in the second position 123, When the second substrate 160 is in the fifth position 163, the fixing part 110 can be located at the seventh position 113, and the second substrate 160 can guide the reaction liquid to the reaction surface 210. At the same time, the sorting part can clean the first substrate 120 to prepare for the next liquid guiding of the first substrate 120; thereafter, referring to Figure 17, after the second substrate 160 guides the reaction liquid to the reaction surface 210, the positions of the first substrate 120 and the second substrate 160 remain unchanged, the third driving part 170 can drive the fixing part 110 to the sixth position 112, and the first substrate 120 can guide the reaction liquid to the reaction surface 210. At the same time, the sorting part can clean the second substrate 160 to prepare for the next liquid guiding of the second substrate 160.

[0127] Furthermore, for the second motion mode described above, the driving direction of the first driving unit 130 can be limited. Specifically, referring to Figures 15-17 and 20, the first substrate 120 and the second substrate 160 can be arranged relative to each other with a spacing therebetween along the second direction Y. The first driving unit 130 can be configured to drive the first substrate 120 and the second substrate 160 to reciprocate along a first direction X1 perpendicular to the second direction Y, such that when the first driving unit 130 drives the first substrate 120 to the first position 122, the second substrate 160 is driven to the fourth position 162, and when the first substrate 120 is driven to the second position 123, the second substrate 160 is driven to the fifth position 163. It will be understood that in this embodiment, the first direction X1 in which the first driving unit 130 drives the first substrate 120 to move can be perpendicular to the second direction Y in which the first substrate 120 and the second substrate 160 are arranged relative to each other. Furthermore, for the embodiment in which the liquid transfer device 100 includes a third driving unit 170, the third driving unit 170 can be configured to drive the fixing unit 110 to reciprocate along the second direction Y. The above arrangement enables the first substrate 120, the second substrate 160, and the fixing portion 110 to be positioned in different positions in different states, making switching between positions convenient and easy to control. Furthermore, depending on the requirements for liquid conduction, in some embodiments, the third driving portion 170 can be configured to drive the fixing portion 110 to reciprocate along the first direction X1.

[0128] Referring to Figures 18-19 and 21 , in an embodiment of the third motion mode, the first substrate 120 and the second substrate 160 may be arranged relative to each other along a first direction X1. The first driving unit 130 may be configured to drive the first substrate 120 and the second substrate 160 to reciprocate along the first direction X1, such that when the first driving unit 130 drives the first substrate 120 to the first position 122, the second substrate 160 is moved to the fifth position 163 relative to the fixed structure 111, and when the first driving unit 130 drives the first substrate 120 to the second position 123 relative to the fixed structure 111, the second substrate 160 is moved to the fourth position 162. It will be appreciated that, unlike the second motion mode described above, in this embodiment, the direction of motion of the first substrate 120 and the second substrate 160 is the same as the relative direction of the first substrate 120 and the second substrate 160. In some embodiments, the fixed portion 110 may be fixed, so that the fixed portion 110 receives reaction solutions from different substrates solely through the movement of the first substrate 120 and the second substrate 160. Depending on the liquid transfer requirements, in other embodiments, the liquid transfer device 100 may also include a third driving unit 170. The third driving unit 170 may be connected to the fixing unit 110 so as to drive the fixing unit 110 to reciprocate along the first direction X1 or the second direction Y.

[0129] Referring to Figures 4 and 22-23 , for an embodiment in which the first drive unit 130 is capable of driving the first substrate 120 and the second substrate 160 to rotate, in an embodiment of the fourth motion mode, the first wall 121 can be planar and parallel to the reaction surface 210. The first drive unit 130 can be configured to drive the first substrate 120 to rotate about an axis perpendicular to the first wall 121, so that the first substrate 120 switches between a first position 122 and a second position 123. The axis can pass through the first wall 121. In other words, in this embodiment, the first substrate 120 switches position by rotating.

[0130] Based on this, referring to Figures 22-25 , the first wall 121 can include a first region 1211 and a second region 1212. The first region 1211 and the second region 1212 can be distributed circumferentially about the axis. When the first substrate 120 is in the first position 122, the first region 1211 can be used to collect the reaction liquid. Correspondingly, when the first substrate 120 is in the second position 123, the first region 1211 can be used to transfer the collected reaction liquid to the reaction surface 210. 22 , in some embodiments, the first substrate 120 may be first located at the first position 122, at which time the first region 1211 may obtain the reaction liquid, and the second region 1212 may transfer the reaction liquid to the reaction surface 210. In one case, the first substrate 120 may be rotated 90° in the clockwise direction as shown in the figure, so that the first region 1211 is located at the position where the second region 1212 was originally located, and the reaction liquid may be transferred to the reaction surface 210. The second region 1212 rotated 90° may be used for liquid distribution or cleaning. After the above operation is completed, the first substrate 120 may be further rotated clockwise to complete other operations, or the first substrate 120 may be rotated 90° counterclockwise to return to the state shown in FIG. 22 , and may perform multiple cyclic reciprocating motions. Referring to FIG. 23 , in another case, the first substrate 120 may be rotated 180° in the clockwise direction as shown in the figure, so that the first region 1211 is located at the upper part of the figure, and the second region 1212 is located at the lower part of the figure. In the lower part of the figure, the upper part within the circular rotation range in the figure can be used for guiding liquid, and the lower part can be used for distributing liquid, and the fixing part 110 and the liquid distributing part 140 can be moved or arranged in different positions accordingly; referring to Figures 24-25, in other embodiments, the first position 122 and the second position 123 can be respectively represented as different rotation positions of the first substrate 120. Specifically, referring to Figure 24, when the first substrate 120 is in the first position 122, the first area 1211 can obtain the liquid distributing part 1 40, the second area 1212 can transfer the reaction liquid to the reaction surface 210. After completing the above actions, referring to Figure 25, the first substrate 120 can be rotated 180° in a clockwise direction to the second position 123. At this time, the first area 1211 can transfer the reaction liquid to the reaction surface 210, and the second area 1212 can obtain the reaction liquid from the liquid distribution part 140; thereafter, the first substrate 120 can continue to rotate 180° in a clockwise direction or 180° in a counterclockwise direction to perform multiple cycles of reciprocating motion.It can be understood that in some embodiments, the first area 1211 and the second area 1212 of the first substrate 120 along the circumference of the axis can be used to obtain the reaction liquid and conduct the liquid, respectively. When the first substrate 120 is located at the first position 122 or the second position 123, there can always be an area that can obtain the reaction liquid and an area that can conduct the liquid, which is beneficial to improving the efficiency of liquid transfer.

[0131] Furthermore, corresponding to the rotation of the first substrate 120 about the axis, the liquid transfer device 100 may also include a third driving unit 170. The third driving unit 170 may be connected to the fixed unit 110, so that the third driving unit 170 can be configured to drive the fixed unit 110 to rotate about the axis, or to drive the fixed unit 110 to move in a direction perpendicular to the rotation axis of the first substrate 120.

[0132] 26 to 28 , based on the configuration in which the first substrate 120 can rotate around an axis, the area of ​​the first wall 121 can be further divided so that the first wall 121 can perform more operations simultaneously. Specifically, in the embodiment of the fifth motion mode, the first wall 121 can further include a third area 1213. Specifically, the first area 1211, the second area 1212, and the third area 1213 can be arranged in sequence along the rotation direction of the first substrate 120. The first substrate 120 can further include a third position 124. Along the rotation direction of the first substrate 120, the first driving unit 130 can drive the first substrate 120 to cyclically rotate in the order of rotating from the first position 122 to the second position 123, from the second position 123 to the third position 124, and from the third position 124 to the first position 122.

[0133] Based on the above configuration, referring to Figures 26-28 , in some embodiments, the liquid transfer device 100 may include a liquid distribution unit 140 and a cleaning unit 150. As the first substrate 120 is sequentially positioned in the first position 122, the second position 123, and the third position 124, the first region 1211, the third region 1213, and the second region 1212 can sequentially receive the reaction liquid from the liquid distribution unit 140; the second region 1212, the first region 1211, and the third region 1213 can sequentially transfer the reaction liquid to the reaction surface 210; and the third region 1213, the second region 1212, and the first region 1211 can sequentially be cleaned by the cleaning unit 150. Specifically, referring to Figures 26-28 , in some embodiments, the clockwise rotation direction of the first substrate 120 can be defined as the first circumferential direction. As for the specific movement mode, for example, taking the initial state when the first substrate 120 is located at the first position 122 as an example, see Figure 26, at this time, the first area 1211 can obtain the reaction liquid of the liquid distribution part 140, the second area 1212 can transfer the reaction liquid to the reaction surface 210, and the third area 1213 can be cleaned by the cleaning part 150; after completing the above actions, see Figure 27, along the first circumferential direction, the first substrate 120 can be rotated to the second position 123, at this time the first area 1211 can The reaction liquid is transferred to the reaction surface 210, and the second area 1212 can be cleaned by the cleaning part 150, and the third area 1213 can obtain the reaction liquid of the liquid distribution part 140; after completing the above action, referring to Figure 28, along the first circumferential direction, the first substrate 120 can be rotated to the third position 124, at which time the first area 1211 can be cleaned by the cleaning part 150, and the second area 1212 can obtain the reaction liquid of the liquid distribution part 140, and the third area 1213 can transfer the reaction liquid to the reaction surface 210. It can be understood that in this embodiment, the first substrate 120 can have three areas and three positions relative to the fixing part 110 in sequence along the circumferential direction of the axis. During the circumferential rotation of the first substrate 120 around the axis, any area can be located in different parts arranged along the circumferential direction in sequence, and each part can perform different operations respectively. The above arrangement enables the liquid transfer device 100 to effectively utilize each operating position by rotating the first substrate 120 when it has multiple operating positions, which is conducive to improving the efficiency of liquid transfer. In addition, without being limited to the configuration of the above-mentioned embodiment, in some embodiments, the liquid transfer device 100 may also include other operating parts, and the total number of each part may correspond to the number of areas, so that different parts of the liquid transfer device 100 can be adapted to flow between different positions.

[0134] Referring to Figures 4 and 29-30, for the embodiment in which the first substrate 120 can rotate circumferentially around the axis, a second substrate 160 can be further configured. Specifically, referring to Figures 29-30, in the embodiment of the sixth motion mode, the first wall 121 can be planar and parallel to the reaction surface 210. The first driving portion 130 can be configured to drive the first substrate 120 to rotate around an axis perpendicular to the first wall 121, so that the first substrate 120 switches positions between the first position 122 and the second position 123. The axis passes through the first wall 121. It can be understood that the configuration of the above-mentioned first driving portion 130 is similar to the configuration of the above-mentioned fourth motion mode, and its function is to enable the first substrate 120 to achieve position switching by rotation.

[0135] Based on the above configuration, referring to Figures 29-30, the liquid transfer device 100 may further include a second substrate 160. The second substrate 160 may include a second wall 161. The first driving unit 130 may be configured to drive the second wall 161 to rotate around an axis so that the second substrate 160 switches positions between a fourth position 162 and a fifth position 163. It is understandable that the configuration of the second substrate 160 is similar to that of the first substrate 120, and the position switching can also be achieved by driving the second wall 161 to rotate by the first driving unit 130. When the second substrate 160 is in the fourth position 162, the second wall 161 is used to obtain the reaction liquid, and when the second substrate 160 is in the fifth position 163, the second wall 161 transfers the reaction liquid to the reaction surface 210. Similarly, in order to improve the stability of the reaction liquid transfer, in some embodiments, the Mohs hardness of the second substrate 160 may be greater than or equal to 5H.

[0136] For the structural form of the second wall 161, see Figures 29-30. In some embodiments, the second wall 161 can be annular, and the second wall 161 can be arranged around the outside of the first wall 121, or the second wall 161 can be arranged around the inside of the first wall 121; depending on the different liquid conduction requirements, see Figure 31. In some embodiments, the second wall 161 can also be semi-annular.

[0137] Based on the above configuration, the fixed portion 110 can be further driven to adapt to the liquid guiding needs. Specifically, referring to Figures 29-30, in some embodiments, the liquid transfer device 100 can also include a third driving portion 170. The third driving portion 170 can be connected to the fixed portion 110 and can drive the fixed portion 110 to switch positions between the sixth position 112 and the seventh position 113 in a direction perpendicular to the axis. When the fixed portion 110 is in the sixth position 112, the fixed structure 111 is spaced apart from the first wall 121. When the fixed portion 110 is in the seventh position 113, the fixed structure 111 is spaced apart from the second wall 161. It can be understood that when the fixed portion 110 is in the sixth position 112, the first wall 121 in the second position 123 can guide the reaction liquid to the reaction surface 210; when the fixed portion 110 is in the seventh position 113, the second wall 161 in the fifth position 163 can guide the reaction liquid to the reaction surface 210.

[0138] For an example of the specific movement of this embodiment, see Figure 29 . Taking the initial state, when the first substrate 120 is in the second position 123, as an example, the first substrate 120 can transfer the reaction liquid to the reaction surface 210. At this time, the second substrate 160 can be in the fourth position 162 and can obtain the reaction liquid from the liquid distribution unit 140. After the first substrate 120 has completed the liquid transfer at the second position 123, see Figure 30 . The first substrate 120 can rotate along the first circumferential direction to the first position 122 and obtain the reaction liquid from the liquid distribution unit 140. At this time, the second substrate 160 can rotate to the fifth position 163 and transfer the reaction liquid to the reaction surface 210. During the circumferential rotation of the first and second substrates 120, 160 along the axis, the third drive unit 170 can drive the fixed unit 110 to move accordingly. For example, after the first substrate 120 has completed liquid transfer and rotated from the second position 123 to the first position 122, and the second substrate 160 has rotated to the fifth position 163, the third driving unit 170 can simultaneously drive the fixed portion 110 to move from the sixth position 112 to the seventh position 113. The above arrangement, through the coordinated operation of the independently rotatable first substrate 120, the second substrate 160, and the movable fixed portion 110, enables the first substrate 120 and the second substrate 160 to simultaneously complete the transition between liquid transfer and liquid extraction during the movement of the fixed portion 110, ensuring that at least one substrate can always obtain the reaction solution and at least one substrate can always transfer the solution, thereby improving the efficiency of liquid transfer.

[0139] Based on different liquid guiding requirements, in multiple embodiments of the above-mentioned six motion modes, in addition to the operations of obtaining the reaction liquid and directing the reaction liquid to the reaction surface 210, other types of operations can be equivalently added or replaced in the liquid transfer device 100. For example, in some embodiments, an operation of cleaning the reaction surface 210 can be further added (which can be understood as adding the finishing part in the above-mentioned embodiment), and corresponding adaptive configurations can be made. In some embodiments, different operations can correspond to different positions; in other embodiments, two or more operations can be integrated at the same position for processing. For example, the first substrate 120 can complete the operations of cleaning the first wall 121 and obtaining the reaction liquid in sequence at the first position 122.

[0140] It should be noted that the above-mentioned multiple embodiments of the six motion modes are merely exemplary embodiments. By combining different embodiments, more types of embodiments can be derived, which will not be described in detail here.

[0141] In order to achieve a better liquid transfer effect and to prevent the first substrate 120 from shaking during the process of transferring the reaction liquid to the reaction surface 210, the structure of the first substrate 120 can be further limited. Specifically, in some embodiments, the material of the first substrate 120 can be glass or ceramic. In this embodiment, using glass or ceramic as the first substrate 120 can meet the Mohs hardness greater than or equal to 5H and have a better liquid transfer effect. Furthermore, using glass or ceramic as the first substrate 120 can also prevent the first substrate 120 from interfering with (for example, dissolving or adsorbing) the sample or reaction liquid. Alternatively, in other embodiments, the first wall 121 can be plasma / corona modified to increase the surface tension of the first wall 121. In this embodiment, by increasing the surface tension of the first wall 121, a better liquid transfer effect can be achieved. Alternatively, in other embodiments, when the first wall 121 is in the second position 123, the distance between it and the reaction surface 210 is twice the thickness of the reaction liquid. Based on the principle of liquid transfer, the sample carrier 200 and the substrate move tangentially relative to each other, generating shear force within a certain gap range (a gap range that satisfies the minimum reaction liquid replacement ratio condition) and directing the reaction liquid to the reaction surface 210. In this embodiment, the spacing between the first wall 121 and the reaction surface 210 is limited to twice the thickness of the reaction liquid. This can prevent the sample carrier 200 from drying out or leaking liquid from the edges, thereby achieving a better liquid transfer effect. It should be noted that, in addition to the first substrate 120, when the liquid transfer device 100 also has other substrates and is also used for liquid transfer, the above-mentioned limitations on the first substrate 120 can also be adaptively applied to other substrates.

[0142] In order to achieve better liquid transfer effects, further limitations may be imposed on the liquid transfer device 100. Specifically, in some embodiments, the liquid transfer device 100 may satisfy one of the following conditions a)-f):

[0143] a) The flatness P of the first substrate 120 satisfies: 0 μm ≤ P ≤ 3 μm;

[0144] b) The first wall 121 and the reaction surface 210 are both planar, and the first wall 121 is parallel to the reaction surface 210 . The first substrate 120 is capable of rotating about an axis perpendicular to the first wall 121 . The area of ​​the first wall 121 is not less than twice the area of ​​the reaction surface 210 .

[0145] c) After the first substrate 120 transfers the reaction liquid to the reaction surface 210 at the second position 123, the first substrate 120 moves along the third direction X2. In a direction parallel to the first wall 121, the cross-section of the first substrate 120 has a first cross-sectional side 125 parallel to the third direction X2. Along the third direction X2, the length of the first cross-sectional side 125 is greater than twice the length of the reaction surface 210.

[0146] d) After the first substrate 120 transfers the reaction liquid to the reaction surface 210 at the second position 123, the first substrate 120 moves along the third direction X2. In a direction parallel to the first wall 121, the cross-section of the first substrate 120 has a second cross-sectional side 126 perpendicular to the third direction X2. In the direction perpendicular to the third direction X2, the length of the second cross-sectional side 126 is greater than the length of the reaction surface 210.

[0147] e) The liquid transfer device 100 further includes a liquid distribution unit 140 and a cleaning unit 150. The liquid distribution unit 140 is configured to distribute the reaction liquid toward the first wall surface 121 when the first substrate 120 is in the first position 122. The cleaning unit 150 is configured to clean the first wall surface 121 after the first substrate 120 transfers the reaction liquid to the reaction surface 210 at the second position 123.

[0148] The first driving unit 130 is configured to drive the first substrate 120 to move along the first direction X1. The cleaning unit 150, the liquid dispensing unit 140, the first substrate 120 and the fixing unit 110 are arranged relative to each other along the first direction X1.

[0149] and / or,

[0150] The first driving unit 130 is configured to drive the first substrate 120 to rotate around an axis perpendicular to the first wall 121 , and the cleaning unit 150 , the liquid distributing unit 140 , and the fixing unit 110 are distributed along the circumference of the axis.

[0151] f) The first driving unit 130 is configured to drive the first substrate 120 to move along the first direction X1. The liquid transfer device 100 further includes a liquid distributing unit 140. The liquid distributing unit 140 is configured to distribute the reaction liquid toward the first wall surface 121 when the first substrate 120 is in the first position 122. The cleaning unit 150 is configured to clean the first wall surface 121 after the first substrate 120 transfers the reaction liquid to the reaction surface 210 at the second position 123.

[0152] When the first substrate 120 is in the first position 122 , the first substrate 120 and the liquid distributing part 140 are arranged relative to each other along a second direction Y perpendicular to the first direction X1 . When the first substrate 120 is in the second position 123 , the first substrate 120 and the fixing part 110 are arranged relative to each other along the second direction Y.

[0153] The above conditions are further explained below.

[0154] Regarding condition a), in some embodiments, based on the principle of transferring the reaction liquid through shear force due to the relative movement of the first substrate 120 and the sample carrier 200, the flatness of the first substrate 120 is no more than 3. This can prevent the first substrate 120 from scratching the sample carrier 200 or causing the sample carrier 200 to leak, thereby achieving a better liquid transfer effect. Preferably, the flatness of the first substrate 120 can be further limited to 2 to achieve a better pipetting effect.

[0155] Regarding condition b), it can be understood that, based on the configuration that the first substrate 120 can rotate around the axis, the first wall 121 and the reaction surface 210 can be arranged parallel and opposite to each other, and the setting of the area of ​​the first wall 121 being not less than twice the area of ​​the reaction surface 210 can facilitate the transfer of liquid from the first wall 121 to the reaction surface 210, and at the same time, a liquid guide area, a liquid distribution area, a cleaning area, etc. can be set, so that the liquid transfer efficiency is higher.

[0156] For conditions c) and d), referring to FIG1-FIG2 , it can be understood that, in some embodiments, the first substrate 120 can be a rectangular parallelepiped, wherein the long side of the rectangular parallelepiped (the first cross-sectional side 125) can be parallel to the direction in which the first substrate 120 moves to the second position 123, and the short side of the rectangular parallelepiped (the second cross-sectional side 126) can be perpendicular to the direction in which the first substrate 120 moves to the second position 123.

[0157] Based on the above configuration. In some embodiments, along the third direction X2, the length of the first cross-sectional side 125 is greater than twice the length of the reaction surface 210, so that when the first substrate 120 moves along the third direction X2, the reaction liquid on the first wall 121 can be fully transferred to the reaction surface 210 through shear force, which is beneficial to improving the efficiency of liquid transfer. In other embodiments, along the direction perpendicular to the third direction X2, the length of the second cross-sectional side 126 is greater than the length of the reaction surface 210, which can ensure that the reaction liquid on the first wall 121 can be transferred to the reaction surface 210, avoiding uneven pipetting. In this embodiment, the length of the second cross-sectional side 126 and the length of the reaction surface 210 can be understood as the width direction dimensions corresponding to the above-mentioned first cross-sectional side 125.

[0158] For condition e), in the embodiments corresponding to this condition, the configuration of the liquid distribution part 140 and the cleaning part 150 can refer to the relevant descriptions of the above-mentioned embodiments. Specifically, referring to Figure 9 or Figure 10, in some embodiments, the first substrate 120 can move in parallel, and the movement direction of the first substrate 120 can be the same as the relative arrangement direction between the cleaning part 150, the liquid distribution part 140, the first substrate 120 and the fixing part 110. Referring to Figures 26 to 28, in other embodiments, the first substrate 120 can rotate around the axis, and the cleaning part 150, the liquid distribution part 140 and the fixing part 110 can be correspondingly distributed circumferentially along the axis. The above two aspects of the settings enable the various operating areas of the liquid transfer device 100 to cooperate with the movement direction of the first substrate 120, thereby making the liquid transfer efficiency higher.

[0159] Regarding condition f), in the embodiments corresponding to this condition, the configuration of the liquid distribution unit 140 and the cleaning unit 150 can be found in the relevant descriptions of the aforementioned embodiments. It will be understood that, referring to Figures 6 and 8 , the first substrate 120 is capable of movement along a first direction X1, and the liquid distribution unit 140 and the fixing unit 110 are both arranged relative to the first substrate 120 along a second direction Y perpendicular to the first direction X1. This arrangement allows the liquid transfer device 100 to easily perform operations such as liquid distribution and liquid transfer while simultaneously moving the first substrate 120, without interfering with each other.

[0160] It is understood that in the liquid transfer device 100 provided in the embodiment of the first aspect, the first driving unit 130 can drive the first substrate 120 to switch between the first position 122 and the second position 123, while the fixing unit 110 can remain stationary. Based on the above solution, other corresponding configurations can also be made.

[0161] Specifically, referring to Figures 32-33 , an embodiment of the second aspect of the present invention further provides a liquid transfer device 300 for directing a reaction liquid to a reaction surface of a sample carrier. Similarly, the liquid transfer device 300 includes a fixing portion, a first substrate 310 , and a first driving portion.

[0162] The specific structure of the liquid transfer device 300 is described below. The fixing portion includes a fixing structure. The fixing structure is used to fix the sample carrier. The fixing portion has a first position 311 and a second position 312 relative to the fixing portion 110. The first substrate 310 includes a first wall surface. When the fixing portion is in the first position 311, the first wall surface is used to obtain the reaction liquid. When the fixing portion is in the second position 312, the first wall surface is used to guide the reaction liquid to the reaction surface. The first driving portion is configured to drive the fixing portion to switch between the first position 311 and the second position 312.

[0163] Likewise, in order to improve the stability of the reaction solution transfer, the Mohs hardness of the first substrate 310 is greater than or equal to 5H.

[0164] It is understood that the liquid transfer device 300 of the second embodiment is similar to the first embodiment, except that, in this embodiment, the first driving unit can drive the fixing unit to switch between the first and second positions, while the first substrate 310 can be fixed. Therefore, in the second embodiment, the liquid transfer device 300 can have similar effects as the liquid transfer device 100 described above.

[0165] Furthermore, based on the above differences, the various embodiments related to the above-mentioned first aspect embodiment can be combined and applied to the liquid transfer device 300 of the second aspect embodiment to have similar effects, which will not be repeated here.

[0166] Correspondingly, embodiments of the third aspect of the present invention further provide a reaction device comprising the liquid transfer device 100 (or liquid transfer device 300) in any of the above embodiments and a sample carrier. In some embodiments, the reaction device can be adapted to react a reaction liquid with a reaction surface on the sample carrier.

[0167] Correspondingly, an embodiment of the fourth aspect of the present invention further provides an analysis device, comprising the above-mentioned reaction device. In some embodiments, the analysis device can be adapted to analyze the reaction surface after reacting with the reaction liquid and provide corresponding analysis results.

[0168] Thanks to the improvements of the liquid transfer device 100 and the liquid transfer device 300, the reaction device of the third embodiment of the present invention and the analysis device of the fourth embodiment of the present invention both have the same technical effects as the liquid transfer device 100 and the liquid transfer device 300 in the above embodiments. Detailed description is omitted here.

[0169] The above are only preferred embodiments of the present invention and are not intended to limit the patent scope of the present invention. All equivalent structural transformations made using the contents of the present invention's description and drawings, or direct / indirect applications in other related technical fields, within the scope of the present invention are included in the patent protection scope of the present invention.

Claims

1. A liquid transfer device for directing a reaction liquid to a reaction surface of a sample carrier, characterized in that: The liquid transfer device comprises: A fixing part, comprising a fixing structure, wherein the fixing structure is used to fix the sample carrier; A first substrate, comprising a first wall surface, wherein the first substrate has a first position and a second position relative to the fixed structure, the first wall surface is used to obtain the reaction liquid when the first substrate is in the first position, and the first wall surface is used to guide the reaction liquid to the reaction surface when the first substrate is in the second position; and A first driving unit is configured to drive the first substrate to switch between the first position and the second position; Wherein, the Mohs hardness of the first substrate is greater than or equal to 5H.

2. The liquid transfer device according to claim 1, characterized in that The first wall surface is plane and parallel to the reaction surface, and the first driving unit is configured to drive the first substrate to translate along a direction parallel to the first wall surface, so that the first substrate switches between the first position and the second position; or, The first wall surface is plane and parallel to the reaction surface, and the first driving unit is configured to drive the first substrate to rotate around an axis perpendicular to the first wall surface, so that the first substrate switches between the first position and the second position; or, The first wall surface is a cylindrical surface, the central axis of the cylindrical surface is parallel to the reaction surface, and the first driving part is configured to drive the first substrate to rotate around the central axis so that the first substrate switches between the first position and the second position.

3. The liquid transfer device according to claim 1, characterized in that The liquid transfer device further includes a liquid distributing unit and a cleaning unit, wherein the liquid distributing unit is configured to distribute the reaction liquid to the first wall surface when the first substrate is in the first position, and the cleaning unit is configured to clean the first wall surface after the first substrate transfers the reaction liquid to the reaction surface at the second position and moves to the first position; or, The first substrate also has a third position relative to the fixed structure, the first driving unit is configured to drive the first substrate to switch between the second position and the third position, the liquid transfer device includes a liquid distribution unit and a cleaning unit, the liquid distribution unit is configured to arrange the reaction liquid toward the first wall surface when the first substrate is in the first position, and the cleaning unit is configured to clean the first wall surface when the first substrate is in the third position.

4. The liquid transfer device according to claim 1, characterized in that The liquid transfer device further includes a second substrate, the second substrate includes a second wall surface, the second substrate has a fourth position and a fifth position relative to the fixed structure, when the second substrate is in the fourth position, the second wall surface is used to obtain the reaction liquid, and when the second substrate is in the fifth position, the second wall surface is used to guide the reaction liquid to the reaction surface; the Mohs hardness of the second substrate is greater than or equal to 5H; The first driving unit is configured to drive the second substrate to switch between the fourth position and the fifth position; Alternatively, the liquid transfer device further includes a second driving unit, and the second driving unit is configured to drive the second substrate to switch positions between the fourth position and the fifth position.

5. The liquid transfer device according to claim 4, characterized in that: The first driving unit is configured to drive the second substrate to the fifth position and be spaced opposite to the fixed structure when the first substrate is in the first position, and to drive the second substrate to the fourth position when the first substrate is in the second position and be spaced opposite to the fixed structure; or, The first driving unit is configured to drive the second substrate to be in the fourth position when the first substrate is in the first position, and to drive the second substrate to be in the fifth position when the first substrate is in the second position; the liquid transfer device also includes a third driving unit, the third driving unit is connected to the fixed unit, and the third driving unit drives the fixed unit to switch positions between a sixth position and a seventh position, when the fixed unit is in the sixth position, the fixed structure is arranged relative to the first wall surface of the first substrate located in the second position, and when the fixed unit is in the seventh position, the fixed structure is arranged relative to the second wall surface of the second substrate located in the fifth position.

6. The liquid transfer device according to claim 4, characterized in that: The first substrate and the second substrate are arranged opposite to each other along a first direction, and the first driving unit is configured to drive the first substrate and the second substrate to reciprocate along the first direction, so that when the first driving unit drives the first substrate to be in the first position, the second substrate is driven to be in the fifth position and opposite to the fixed structure, and when the first driving unit drives the first substrate to be in the second position and opposite to the fixed structure, the second substrate is driven to be in the fourth position; or, The first substrate and the second substrate are arranged relative to each other along a second direction, and the first driving unit is configured to drive the first substrate and the second substrate to reciprocate along a first direction perpendicular to the second direction, so that the first driving unit drives the second substrate to the fourth position when the first substrate is in the first position, and drives the second substrate to the fifth position when the first substrate is in the second position; the liquid transfer device also includes a third driving unit, the third driving unit is connected to the fixing unit, and drives the fixing unit to switch positions between a sixth position and a seventh position, when the fixing unit is in the sixth position, the fixing structure is arranged relative to the first wall surface of the first substrate located at the second position, and when the fixing unit is in the seventh position, the fixing structure is arranged relative to the first wall surface of the second substrate located at the fifth position.

7. The liquid transfer device according to claim 1, characterized in that: The first wall surface is plane and parallel to the reaction surface, and the first driving unit is configured to drive the first substrate to rotate around an axis perpendicular to the first wall surface, so that the first substrate switches between the first position and the second position; the axis passes through the first wall surface; The first wall surface includes a first area and a second area, the first area and the second area are distributed circumferentially along the axis, when the first substrate is in the first position, the first area is used to obtain the reaction liquid, when the first substrate is in the second position, the first area is used to transfer the obtained reaction liquid to the reaction surface.

8. The liquid transfer device according to claim 7, characterized in that The first wall surface further includes a third area, and the first area, the second area and the third area are arranged in sequence along the rotation direction of the first substrate; The first substrate further includes a third position, and along the rotation direction of the first substrate, the first driving unit drives the first substrate to cyclically rotate in the order of rotating from the first position to the second position, rotating from the second position to the third position, and rotating from the third position to the first position; The liquid transfer device includes a liquid distribution part and a cleaning part; when the first substrate is located at the first position, During the process of the second position and the third position, the first area, the third area and the second area successively obtain the reaction liquid from the liquid distribution part, the second area, the first area and the third area successively transfer the reaction liquid to the reaction surface, and the third area, the second area and the first area are successively cleaned by the cleaning part.

9. The liquid transfer device according to claim 1, characterized in that: The first wall surface is plane and parallel to the reaction surface, and the first driving unit is configured to drive the first substrate to rotate around an axis perpendicular to the first wall surface, so that the first substrate switches between the first position and the second position; the axis passes through the first wall surface; The liquid transfer device further includes a second substrate, the second substrate includes a second wall surface, the second wall surface is annular and is arranged around the outside of the first wall surface, the first driving unit is configured to drive the second wall surface to rotate around the axis, so that the second substrate switches between a fourth position and a fifth position, when the second substrate is in the fourth position, the second wall surface is used to obtain the reaction liquid, and when the second substrate is in the fifth position, the second wall surface transfers the reaction liquid to the reaction surface; the Mohs hardness of the second substrate is greater than or equal to 5H; The liquid transfer device also includes a third driving part, which is connected to the fixed part and can drive the fixed part to switch between a sixth position and a seventh position along a direction perpendicular to the axis. When the fixed part is in the sixth position, the fixed structure is opposite to the first wall surface. When the fixed part is in the seventh position, the fixed structure is opposite to the second wall surface.

10. The liquid transfer device according to claim 1, characterized in that: The first substrate is made of glass or ceramic; or, The first wall surface is plasma / corona modified to increase the surface tension of the first wall surface; or, When the first wall surface is at the second position, the distance between the first wall surface and the reaction surface is twice the thickness of the reaction liquid.

11. The liquid transfer device according to claim 1, characterized in that: The liquid transfer device satisfies one of the following conditions a)-f): a) The flatness P of the first substrate satisfies: 0um≤P≤3um; b) the first wall surface and the reaction surface are both planes, and the first wall surface is parallel to the reaction surface, the first substrate can rotate around an axis perpendicular to the first wall surface, and the area of ​​the first wall surface is not less than twice the area of ​​the reaction surface; c), the first driving unit drives the first substrate to move along a third direction to the second position, and along a direction parallel to the first wall surface, a cross section of the first substrate has a first cross section side parallel to the third direction, and along the third direction, a length of the first cross section side is greater than twice the length of the reaction surface; d), the first driving unit drives the first substrate to move along the third direction to the second position, along the direction parallel to the first wall, the cross section of the first substrate has a second cross section side perpendicular to the third direction, and along the direction perpendicular to the third direction, the length of the second cross section side is greater than the length of the reaction surface; e), the liquid transfer device further includes a liquid distributing unit and a cleaning unit, the liquid distributing unit is configured to distribute the reaction liquid to the first wall surface when the first substrate is in the first position, and the cleaning unit is configured to clean the first wall surface after the first substrate transfers the reaction liquid to the reaction surface at the second position; The first driving unit is configured to drive the first substrate to move along a first direction, and the cleaning unit, the liquid distributing unit, the first substrate and the fixing unit are arranged relatively spaced apart along the first direction. and / or, The first driving part is configured to drive the first substrate to rotate around an axis perpendicular to the first wall surface, and the cleaning part, the liquid distributing part and the fixing part are distributed along the circumference of the axis; f), the first driving unit is configured to drive the first substrate to move along a first direction, the liquid transfer device further comprises a liquid distributing unit, the liquid distributing unit is configured to arrange the reaction liquid on the first wall surface when the first substrate is in the first position, and the cleaning unit is configured to clean the first wall surface after the first substrate transfers the reaction liquid to the reaction surface at the second position; When the first substrate is in the first position, the first substrate and the liquid distributing part are arranged opposite to each other along a second direction perpendicular to the first direction. When the first substrate is in the second position, the first substrate and the fixing part are arranged opposite to each other along the second direction.

12. A liquid transfer device for directing a reaction liquid to a reaction surface of a sample carrier, characterized in that: The liquid transfer device comprises: A fixing portion, comprising a fixing structure, the fixing structure being used to fix the sample carrier, the fixing portion having a first position and a second position relative to the fixing structure; a first substrate, comprising a first wall surface, wherein the first wall surface is used to obtain the reaction liquid when the fixing portion is in the first position, and the first wall surface is used to guide the reaction liquid to the reaction surface when the fixing portion is in the second position; and a first driving unit configured to drive the fixing unit to switch between the first position and the second position; Wherein, the Mohs hardness of the first substrate is greater than or equal to 5H.

13. A reaction device, characterized in that: It comprises the liquid transfer device according to any one of claims 1 to 12 and the sample carrier.

14. An analysis device, characterized in that: include: The reaction device according to claim 13; as well as Detection device.

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