Apongopus feeding feed composition, preparation method, product and application

Abstract

The invention provides an aspongopus feeding feed composition, a preparation method, a product and application, and belongs to the technical field of animal feed. The composition consists of the following components in parts by weight: 1-9 parts of a cucurbitaceae plant extract, 1-9 parts of compound amino acid powder, 1-5 parts of glucose powder, 1-3 parts of organic acid and 0.01-2 parts of multivitamin element powder, the compound amino acid powder consists of 10 amino acids including threonine, and the threonine accounts for 20-40% of the compound amino acid powder in percentage by weight; the organic acid is composed of lauric acid, linolenic acid and shikimic acid, and the shikimic acid accounts for 30-50% of the organic acid in percentage by weight. The aspongopus breeding feed prepared from the composition can effectively improve the survival rate and weight of aspongopus, the content of alcohol-soluble extracts and the anti-tumor effect of the aspongopus extracts.

Description

Technical Field

[0001] This invention belongs to the field of animal feed technology, and relates to a feeding composition for stink bugs, its preparation method, product, and application. Background Technology

[0002] Aspongopus chinesis Dallas, also known as the black-scented bug, melon-black bug, or fart-board bug, is an insect belonging to the family Aspidae in the class Insecta of the phylum Arthropoda. The dried whole insect of Aspongopus chinesis Dallas is a valuable traditional Chinese medicine. Aspongopus chinesis Dallas has the effects of regulating qi and relieving pain, warming the middle jiao and invigorating yang, and is mainly used to treat symptoms such as abdominal distension, liver and stomach qi pain, impotence, and lower back and knee pain.

[0003] The existing technology article "The biosynthetic products of Chinese insect medicine, Aspongopus chinensis" (Fitoterapia, 83 (2012) 754-758) points out that the extract of Aspongopus chinensis has an inhibitory effect on different types of tumor cells. The reason for this is that Aspongopus chinensis has a unique biological metabolic pathway, which produces compounds with specific structures. These compounds are one of the possible reasons why the extract of Aspongopus chinensis has anti-tumor effects.

[0004] In recent years, due to the extensive use of pesticides, the number of wild stink bugs has been declining, and relying solely on collecting wild stink bugs is no longer sufficient to meet the development needs of the traditional Chinese medicine industry. Therefore, the technology related to the large-scale breeding of stink bugs, including stink bug feed and breeding methods, has become a research hotspot.

[0005] Traditional methods of raising stink bugs mainly involve building breeding cages and then planting fruits and vegetables inside as feed. This method has the following disadvantages: ① The ripening of fruits and vegetables is greatly affected by seasonal factors; ② Using only fruits and vegetables as feed results in insufficient nutrition, leading to slow growth and low survival rates for the stink bugs. Therefore, stink bugs raised using this traditional method are costly and of poor quality.

[0006] To overcome the above problems and improve the quality of stink bugs while reducing breeding costs, existing technologies have developed feed for the fully artificial breeding of stink bugs.

[0007] Chinese invention patent CN107549518A discloses a breeding feed for improving the quality of stink bugs. This feed consists of 10-20 parts bitter zucchini juice, 10-20 parts bitter melon juice, 50-70 parts water, 1-3 parts sucrose, 0.1-0.5 parts potassium sorbate, and 0.1-0.5 parts pectin. The nutritional composition of this formula is relatively reasonable and can improve the disease resistance and quality of stink bugs.

[0008] Chinese invention patent CN107594233A discloses a liquid feed for the breeding of stink bugs. The feed consists of 30-45 parts watermelon juice, 0.01-0.1 parts inorganic zinc, 5-11 parts compound probiotics, 5-10 parts feed additives, 0.5-2 parts glucose syrup, 0.1-0.3 parts potassium sorbate, 0.01-0.1 parts sodium benzoate, and 15-30 parts water. This feed formula can improve the disease resistance of stink bugs.

[0009] Chinese invention patent CN108338292A discloses a feed for raising stink bugs and its preparation method. The feed comprises the following components by weight: 20-35 parts watermelon pulp, 50-70 parts water, 0.1-0.5 parts sodium benzoate, and 0.5-1.5 parts honey. This feed provides balanced nutrition and low fat content to stink bugs, and can further improve the quality of adult stink bugs by enhancing their disease resistance.

[0010] Chinese invention patent CN102696915A discloses a semi-artificial breeding feed for stink bugs. The feed consists of 250-350 parts pumpkin pulp, 8-12 parts agar, 8-12 parts sucrose, 4-6 parts potassium sorbate, 4-6 parts ascorbic acid, and 550-650 parts distilled water. This feed is mainly used for raising stink bugs during seasons when feed is scarce, and the quality of the farmed stink bugs is not much different from that of wild ones.

[0011] However, existing technologies are still unable to provide a suitable feed for stink bugs that can be fed to all stages of the insect life, from nymph to adult, while also increasing the weight and extract content of stink bugs and enhancing the anti-cancer effects of stink bug extracts. Summary of the Invention

[0012] In view of the above-mentioned problems existing in the prior art, the purpose of this invention is to provide a nine-scented insect feeding composition, preparation method, product, and application. The inventors, through the rational compounding of compound amino acid powder and multi-dimensional element powder, and the addition of shikimic acid as a component of the nine-scented insect feeding feed, have significantly improved the problems of low survival rate, slow weight gain, and low alcohol-soluble content in existing nine-scented insect feeding feeds.

[0013] To achieve the above-mentioned objectives, in a first aspect, the present invention provides a feeding composition for stink bugs, comprising the following components by weight: 1-10 parts of Cucurbitaceae plant extract, 1-10 parts of compound amino acid powder, 1-5 parts of glucose powder, 1-3 parts of organic acid, and 0.01-2 parts of multi-dimensional element powder; The composite amino acid powder is composed of isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, valine, arginine, and histidine, wherein the threonine accounts for 20%-40% of the weight percentage of the composite amino acid powder. The multi-dimensional element powder is composed of calcium lactate, sodium chloride, potassium chloride, sodium dihydrogen phosphate, magnesium sulfate, zinc sulfate, manganese sulfate, ferrous sulfate and selenium yeast powder. The organic acid is composed of lauric acid, linolenic acid and shikimic acid, wherein the shikimic acid accounts for 30%-50% of the organic acid by weight.

[0014] Furthermore, the species of Cucurbitaceae plants are selected from one or more of the following: watermelon, pumpkin, loofah, winter melon, bitter melon, cucumber, cantaloupe, and gourd. The parts of the cucurbitaceous plants are selected from one or more of the stems, leaves, flowers, and fruits.

[0015] Furthermore, the nine-fragrant insect feeding composition comprises the following components by weight: 3-7 parts of cucurbitaceous plant extract, 3-7 parts of compound amino acid powder, 2-4 parts of glucose powder, 1-3 parts of organic acid, and 1 part of multi-vitamin powder.

[0016] Furthermore, the nine-fragrant insect feeding composition consists of the following components by weight: 5 parts of cucurbitaceous plant extract, 5 parts of compound amino acid powder, 3 parts of glucose powder, 2 parts of organic acid, and 1 part of multi-vitamin powder.

[0017] Furthermore, the weight ratio of isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, valine, arginine, and histidine in the composite amino acid powder is 1-20:1-20:1-20:1-20:1-20:20-40:1-20:1-20:1-20:1-20.

[0018] Furthermore, the weight ratio of isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, valine, arginine, and histidine in the composite amino acid powder is 9-11:4-6:8-11:4-6:9-11:20-40:4-6:4-6:9-11:9-12.

[0019] Furthermore, the weight ratio of isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, valine, arginine, and histidine in the composite amino acid powder is 10:5:10:5:10:30:5:5:10:10.

[0020] Furthermore, the weight ratio of calcium lactate, sodium chloride, potassium chloride, sodium dihydrogen phosphate, magnesium sulfate, zinc sulfate, manganese sulfate, ferrous sulfate and selenium yeast powder in the multidimensional element powder is 30-70:1-20:1-10:1-10:1-10:1-10:1-10:1-10:1-10:1-10:1-10.

[0021] Furthermore, the weight ratio of calcium lactate, sodium chloride, potassium chloride, sodium dihydrogen phosphate, magnesium sulfate, zinc sulfate, manganese sulfate, ferrous sulfate and selenium yeast powder in the multidimensional element powder is 45-55:8-12:4-6:7-9:1-7:5-7:2-8:4-8:4-8.

[0022] Furthermore, the weight ratio of calcium lactate, sodium chloride, potassium chloride, sodium dihydrogen phosphate, magnesium sulfate, zinc sulfate, manganese sulfate, ferrous sulfate, and selenium yeast powder in the multidimensional element powder is 50:10:5:8:3:6:5:6:7.

[0023] Furthermore, the weight ratio of lauric acid, linolenic acid and shikimic acid in the organic acid is 1-30:1-50:1-70.

[0024] Furthermore, the weight ratio of lauric acid, linolenic acid and shikimic acid in the organic acid is 20-28:30-42:30-50.

[0025] Furthermore, the weight ratio of lauric acid, linolenic acid and shikimic acid in the organic acid is 24:36:40.

[0026] Secondly, the present invention provides a feed for nine-scented insects, which is prepared from the above-mentioned feed composition for nine-scented insects as raw materials.

[0027] Furthermore, the feed for the nine-fragrant insects also includes supplementary ingredients.

[0028] Furthermore, the excipients include one or more of solvents, preservatives, or antioxidants.

[0029] Thirdly, the present invention provides a method for preparing stink bug feed using the above-mentioned stink bug feed composition, comprising the following steps: The product is obtained by mixing Cucurbitaceae plant extract, compound amino acid powder, glucose powder, and multivitamin powder in a certain proportion.

[0030] Fourthly, the present invention provides the application of the above-mentioned stink bug feeding composition or stink bug feeding feed or feeding feed prepared by the above-mentioned method in the production of insect feed products.

[0031] Compared with the prior art, the present invention has the following beneficial effects: 1. This invention provides a novel feed composition for feeding stink bugs. By rationally selecting the types and proportions of amino acids, minerals and other components in the composition, especially by adding shikimic acid, the survival rate of adult stink bugs, the weight of stink bug nymphs and adults, and the content of alcohol-soluble extracts are effectively improved.

[0032] 2. Cell experiments showed that the extract of stink bugs fed with the stink bug feed provided by this invention had a better inhibitory effect on the activity of mouse colon cancer cells.

[0033] 3. The preparation method of this nine-fragrant insect feeding composition is simple. It can be mixed with water to obtain a complete nutritional feed suitable for the entire period of nine-fragrant insect nymphs to adults, which is conducive to the large-scale breeding of nine-fragrant insects. Detailed Implementation

[0034] Terminology and Declarations of this Invention: 1. Articles “a,” “a kind,” and “the”: These include plural objects unless otherwise explicitly specified as a single (kind) object.

[0035] 2. Numerical Range: Unless otherwise expressly stated, all ranges or ratios disclosed herein shall be construed as including any and all subranges or subratios contained herein. For example, a stated range or ratio of 1 to 30 shall be considered to be included between the minimum value of 1 and the maximum value of 30, and includes any subranges or subratios, integers, decimals, or subranges or subratios consisting of integers or decimals, including endpoints.

[0036] The following non-limiting embodiments are intended to enable those skilled in the art to gain a more comprehensive understanding of the present invention, but do not limit the invention in any way. The following content is merely an exemplary description of the scope of protection claimed by the present invention, and those skilled in the art can make various changes and modifications to the present invention based on the disclosed content, and such changes should also fall within the scope of protection claimed by the present invention.

[0037] The present invention will be further described below by way of specific embodiments. Unless otherwise specified, all chemical reagents used in the embodiments of the present invention were obtained through conventional commercial means. Unless otherwise specified, all contents mentioned below are mass contents. Unless otherwise specified, it is understood that the process was carried out at room temperature.

[0038] In the following examples, some of the reagents were sourced from Table 1: Table 1

[0039] The following examples illustrate the preparation methods of Cucurbitaceae plant extracts.

[0040] Method 1: The dried winter melon vines and dried pumpkin leaves were sorted to remove impurities, washed and dried, crushed and passed through a 10-mesh sieve to obtain coarse powder. The winter melon vine (coarse powder) and pumpkin leaves (coarse powder) were mixed with 4 times the amount of water and extracted twice by ultrasound (power: 2.4kW, frequency: 28.5kHz), 4 hours each time. The juice was filtered and concentrated under reduced pressure (70℃) to two-thirds of the original volume to obtain a clear extract.

[0041] Juice is extracted from the whole, unripe watermelon fruit (inferior or weak fruit discarded after thinning), filtered through gauze, and mixed evenly with the above-mentioned clear extract. The mixture is then spray-dried (inlet air temperature 140-170℃, outlet air temperature approximately 80-90℃), ground into a fine powder, and passed through a 50-mesh sieve to obtain Cucurbitaceae plant extract 1.

[0042] The weight ratio of the above-mentioned immature watermelon whole fruit, winter melon vine (coarse powder) and pumpkin leaves (coarse powder) is 5:1:1.

[0043] Method 2: Dried loofah vines and leaves were sorted to remove impurities, washed, dried, and crushed through a 10-mesh sieve to obtain coarse powder. Fresh, ripe pumpkin pulp, dried loofah vine (coarse powder), and dried loofah leaves (coarse powder) were mixed with three times their weight of water. The weight ratio of fresh, ripe pumpkin pulp, dried loofah vine (coarse powder), and dried loofah leaves (coarse powder) was 1:1:1. The mixture was extracted twice using ultrasound (power: 2.4kW, frequency: 28.5kHz), 2 hours each time. The extract was filtered, concentrated under reduced pressure (70℃) to half its original volume, spray-dried (inlet air 140-170℃, outlet air 80-90℃), ground finely, and passed through a 50-mesh sieve to obtain Cucurbitaceae plant extract 2.

[0044] Method 3: Finely chop fresh, ripe pumpkin flesh, fresh pumpkin vines, and fresh pumpkin leaves, and mix them with water. The weight ratio of fresh, ripe pumpkin flesh, fresh pumpkin vines, fresh pumpkin leaves, and water is 3:2:1:6. Extract the juice using a juicer, and filter the juice through cheesecloth. Spray-dry the juice (inlet air 140-170℃, outlet air 80-90℃), grind it into a fine powder, and pass it through a 50-mesh sieve to obtain Cucurbitaceae plant extract 3.

[0045] Method 4: Take fresh winter melon pulp, juice it using a juicer, and filter the juice through gauze. Spray dry the juice (inlet air 140-170℃, outlet air 80-90℃), grind it into a fine powder, and pass it through a 50-mesh sieve to obtain Cucurbitaceae plant extract 4.

[0046] Examples 1-5 provide a feeding composition for stink bugs, the composition of which is shown in Table 2 (unit: parts by weight).

[0047] Table 2

[0048] In Examples 1-5, the cucurbitaceous plant extracts were all Cucurbitaceous Plant Extract 1. The weight percentages of each component in the compound amino acid powder were: isoleucine 10%, leucine 5%, lysine 10%, methionine 5%, phenylalanine 10%, threonine 30%, tryptophan 5%, valine 5%, arginine 10%, and histidine 10%. The weight percentages of each component in the organic acid powder were: lauric acid 24%, linolenic acid 36%, and shikimic acid 40%. The weight percentages of each component in the multivitamin powder were: calcium lactate 50%, sodium chloride 10%, potassium chloride 5%, potassium dihydrogen phosphate 8%, magnesium sulfate 3%, zinc sulfate 6%, manganese sulfate 5%, ferrous sulfate 6%, and selenium yeast powder 7%.

[0049] Compared with Example 1, Examples 6, 7, Comparative Examples 1, and 2 are identical except for the composition of the composite amino acid powder. The composition of the composite amino acid powder in Examples 1, 6, 7, Comparative Examples 1, and 2 is shown in Table 3 ("-" indicates that the component is not present).

[0050] Table 3

[0051] Compared with Example 1, Examples 8, 9, 10, and Comparative Example 3 differ only in the composition of the multidimensional element powder; all other aspects are the same. The composition of the multidimensional element powder in Examples 1, 8, 9, 10, and Comparative Example 3 is shown in Table 4 ("-" indicates that the component is not present).

[0052] Table 4

[0053] Compared with Example 1, Examples 11, 12, 4, and 5 differ only in the composition of the organic acid; all other aspects are the same. The composition of the multi-dimensional element powders of Examples 1, 11, 12, 4, and 5 is shown in Table 5 ("-" indicates that the component is not present).

[0054] Table 5

[0055] Compared with Example 1, Examples 13, 14, and 15 differ only in the types of Cucurbitaceae plant extracts used. The types of Cucurbitaceae plant extracts used in Examples 13, 14, and 15 are Cucurbitaceae plant extract 2, Cucurbitaceae plant extract 3, and Cucurbitaceae plant extract 4, respectively.

[0056] Comparative Example 6 The Chinese invention patent CN107549518A provides a feed for stink bugs. The formula is as follows: 15 parts bitter zucchini juice, 15 parts bitter melon juice, 6 parts water, 2 parts sucrose, 0.3 parts potassium sorbate, and 0.3 parts pectin are mixed together to obtain a liquid feed for stink bugs.

[0057] Preparation Example A method for preparing stink bug feed from the stink bug feeding compositions provided in Examples 1-15 and Comparative Examples 1-5. The specific steps are as follows.

[0058] The product is obtained by mixing Cucurbitaceae plant extract, compound amino acid powder, glucose powder, organic acid and multi-dimensional element powder in a certain proportion.

[0059] Effect evaluation 1. Evaluation of the effects of different feed compositions for *Stachys affinis* on the quality of *Stachys affinis*.

[0060] 1.1 Experimental Methods The nymphs of the stink bug used in this experiment were sourced from the stink bug breeding base of Yunnan Mingyang Pharmaceutical Co., Ltd.

[0061] Specifically, stink bug eggs were collected from parental stink bugs laid on the same day. These eggs were then randomly grouped into groups of 50 and placed in an artificial climate chamber under identical conditions (temperature 28±2℃, humidity 75±5%RH, 16h light: 8h dark cycle). After hatching into first-instar nymphs, each group was provided with a specific stink bug feed as the sole and sufficient source of food and water. The timeline was started from the date all eggs hatched into first-instar nymphs (the earliest and latest hatching dates did not differ by more than two days). The weight of the stink bugs was recorded on day 30. After 60 days of rearing, when all stink bugs had developed into adults, they were harvested and tested.

[0062] The experimental groups are as follows: Traditional group: using loofah vine juice as feed for raising stink bugs.

[0063] Examples 1-15 and Comparative Examples 1-5: The *Stachys affinis* feeding compositions provided in Examples 1-15 and Comparative Examples 1-5 were respectively mixed with water to prepare solutions, which were then fed to the *Stachys affinis*. The weight ratio of the *Stachys affinis* feeding composition to water was 1:4.

[0064] Comparative Example 6 (also known as the prior art group): Nine-scented insects were fed with liquid feed prepared according to the formula described in Comparative Example 6.

[0065] 1.2 Experimental Results 1.2.1 After 60 days of rearing, count the number of surviving adult stink bugs and calculate the survival rate.

[0066] Survival rate = Number of surviving nymphs after 60 days of rearing ÷ Number of nymphs before rearing × 100%.

[0067] The results are shown in Table 6.

[0068] Table 6

[0069] It is evident that the feeding composition for *Symplocos chinensis* provided in each embodiment can be used as feeding feed for *Symplocos chinensis* nymphs / adults, with a survival rate of 98%-100%, while the prior art (comparative examples 6 groups) and the traditional group did not achieve this survival rate.

[0070] 1.2.2 Calculate the average weight of adult stink bugs.

[0071] After the 30th day of rearing and the completion of the survival rate determination, the surviving adult stink bugs were weighed using an electronic analytical balance (minimum precision 0.1 mg), and the average weight and standard deviation of the adult stink bugs were calculated. The results are shown in Table 7.

[0072] Table 7

[0073] Where 'a' indicates that, compared with the traditional group, there is a significant difference in the same column of data and p < 0.05.

[0074] It is evident that the feeding composition for *Stachys affinis* provided in the various embodiments of the present invention, when used to feed *Stachys affinis*, resulted in increased nymph and adult weights after 30 and 60 days of feeding compared to the traditional group and each comparative group.

[0075] 1.2.3 Detect the weight content of alcohol-soluble extracts from adult stink bugs.

[0076] After the weight of the adult stink bugs was measured, each group of stink bugs was euthanized.

[0077] The extract content of *Stachys affinis* was determined according to the extract determination method under the quality standard of *Stachys affinis* in Part I of the Chinese Pharmacopoeia. The specific procedure is as follows.

[0078] (1) Prepare a dilute ethanol solution.

[0079] Dilute 529 mL of anhydrous ethanol with water to 1000 mL to obtain the solution. This solution contains 49.5%-50.5% ethanol at 20°C.

[0080] (2) Determination of the content of the extract of *Symplocos chinensis* by cold soaking method.

[0081] The test sample of *Symplocos edulis* was crushed, passed through a No. 2 sieve, and mixed evenly. Approximately 4 g of the test sample was accurately weighed and placed in a 250 mL Erlenmeyer flask. 100 mL of the dilute ethanol obtained in (1) was accurately added, the flask was sealed, and the sample was allowed to cool. The sample was shaken frequently for the first 6 hours, then allowed to stand for 18 hours. The sample was quickly filtered using a dry filter, and the filtrate and residue were retained separately. 20 mL of the filtrate was accurately measured and placed in an evaporating dish dried to constant weight. The filtrate was evaporated to dryness on a water bath, dried at 105 °C for 3 hours, and then cooled in a desiccator for 30 minutes. The weight was quickly and accurately determined, and the content of alcohol-soluble extracts from *Symplocos edulis* (by weight percentage) was calculated.

[0082] The content of alcohol-soluble extract of *Stachys affinis* = weight of alcohol-soluble extract of *Stachys affinis* ÷ weight of *Stachys affinis* sample × 100%.

[0083] The results are shown in Table 8.

[0084] Table 8

[0085] As shown in Table 8, when using the traditional group's loofah vine juice as feed for stink bugs, the alcohol-soluble extract content was slightly lower than the standard of 10.0% stipulated in Part I of the Chinese Pharmacopoeia, reaching only 9.5%. In contrast, the stink bug feed provided in the various embodiments of this invention can increase the alcohol-soluble extract content of adult stink bugs to 13.0%-16.4%, all higher than the control groups.

[0086] 2. Evaluation of the antitumor effects of extracts from *Symplocos chinensis* fed with different diets.

[0087] 2.1 Preparation of anti-tumor extract from *Corydalis yanhusuo*.

[0088] The *Symplocos chinensis* filter residues obtained in the effect evaluation section 1.2.3(2) were subjected to ultrasonic extraction using a mixed solvent of cyclohexane and ethyl acetate in a volume ratio of 1:1. The extraction temperature was controlled between 30-40℃, the solid-liquid ratio was 1:10, and the extraction time was 1.5h. After extraction, the residues were filtered, and the solvent was recovered by vacuum distillation to obtain a secondary extract of *Symplocos chinensis*. The secondary extract of *Symplocos chinensis* was mixed with the alcohol-soluble extract at a weight ratio of 1:2 to obtain the antitumor extract of *Symplocos chinensis*.

[0089] 2.2 Cell culture and anti-tumor efficacy detection methods.

[0090] CT26.wt mouse colon cancer cells were purchased from Shenger Biotechnology (hereinafter referred to as CT26 cells) and cultured and passaged according to the standard procedures provided by ATCC. During the logarithmic growth phase, CT26 cells were collected and resuspended, and the cell density was adjusted to 5000 cells / 100μL using culture medium. 100μL of sterile water was added to the wells around the edge of a 96-well plate, and 100μL of the adjusted cell suspension was added to the remaining 60 wells. The plates were cultured for 24 hours. Gradual concentrations of *Cypripedium spp.* antitumor extract culture medium were prepared (0, 2.5, 5, 10, 20, 40, 80, 160, 320, and 640 mg / L, a total of 10 concentrations).

[0091] After CT26 cells were cultured in plates for 24 hours, the culture medium was discarded, and 100 μL of a gradient weight concentration of *Cynanchum paniculatum* antitumor extract culture medium was added to each well. The cells were then cultured for another 12 hours. After 12 hours, the viability of the CT26 cells was assessed using a CCK-8 assay kit (purchased from Beyotime Biotechnology). The absorbance was measured using a microplate reader according to the kit's instructions. The results were then used to fit a curve using Graphpad Prism software and calculate the IC50 value.

[0092] 2.3 Experimental Results.

[0093] Nine-scented insects were fed with the feeds provided by the conventional group, Examples 1-15, and Comparative Examples 1-6. The antitumor effects (IC50 values) of the nine-scented insect antitumor extracts prepared from the insects against CT26 cells are shown in Table 9 below: Table 9

[0094] It is evident that the extracts of the stink bugs fed with the stink bug feed provided in the various embodiments of the present invention have a better anti-tumor effect on CT26.

[0095] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, and is not intended to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions made by those skilled in the art to the technical solution of the present invention do not depart from the essence and scope of the technical solution of the present invention.

Claims

1. A feed composition for feeding stink bugs, characterized in that, It consists of the following components by weight: 1-9 parts of Cucurbitaceae plant extract, 1-9 parts of compound amino acid powder, 1-5 parts of glucose powder, 1-3 parts of organic acid, and 0.01-2 parts of multi-dimensional element powder; The composite amino acid powder is composed of isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, valine, arginine, and histidine, wherein the threonine accounts for 20%-40% of the weight percentage of the composite amino acid powder. The multi-dimensional element powder is composed of calcium lactate, sodium chloride, potassium chloride, sodium dihydrogen phosphate, magnesium sulfate, zinc sulfate, manganese sulfate, ferrous sulfate and selenium yeast powder. The organic acid is composed of lauric acid, linolenic acid and shikimic acid, wherein the shikimic acid accounts for 30%-50% of the organic acid by weight.

2. The nine-fragrant insect feeding composition according to claim 1, characterized in that, The species of the Cucurbitaceae plant are selected from one or more of watermelon, pumpkin, loofah, winter melon, bitter melon, cucumber, cantaloupe, and gourd; the parts of the Cucurbitaceae plant are selected from one or more of stem, leaf, flower, and fruit.

3. The nine-fragrant insect feeding composition according to claim 1, characterized in that, It consists of the following components by weight: 3-7 parts of Cucurbitaceae plant extract, 3-7 parts of compound amino acid powder, 2-4 parts of glucose powder, 1-3 parts of organic acid, and 1 part of multi-dimensional element powder.

4. The nine-fragrant insect feeding composition according to claim 3, characterized in that, It consists of the following components by weight: Five parts of Cucurbitaceae plant extract, five parts of compound amino acid powder, three parts of glucose powder, two parts of organic acid, and one part of multivitamin powder.

5. The nine-fragrant insect feeding composition according to claim 1, characterized in that, The weight ratio of isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, valine, arginine and histidine in the compound amino acid powder is 1-20:1-20:1-20:1-20:1-20:20-40:1-20:1-20:1-20:1-20; The weight ratio of calcium lactate, sodium chloride, potassium chloride, sodium dihydrogen phosphate, magnesium sulfate, zinc sulfate, manganese sulfate, ferrous sulfate and selenium yeast powder in the multidimensional element powder is 30-70:1-20:1-10:1-10:1-10:1-10:1-10:1-10:1-10:1-10; The weight ratio of lauric acid, linolenic acid and shikimic acid in the organic acids is 1-30:1-50:1-70.

6. The nine-fragrant insect feeding composition according to claim 5, characterized in that, The weight ratio of isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, valine, arginine and histidine in the compound amino acid powder is 9-11:4-6:8-11:4-6:9-11:20-40:4-6:4-6:9-11:9-12. The weight ratio of calcium lactate, sodium chloride, potassium chloride, sodium dihydrogen phosphate, magnesium sulfate, zinc sulfate, manganese sulfate, ferrous sulfate and selenium yeast powder in the multidimensional element powder is 45-55:8-12:4-6:7-9:1-7:5-7:2-8:4-8:4-8; The weight ratio of lauric acid, linolenic acid and shikimic acid in the organic acids is 20-28:30-42:30-50.

7. The nine-fragrant insect feeding composition according to claim 6, characterized in that, The weight ratio of isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, valine, arginine and histidine in the compound amino acid powder is 10:5:10:5:10:30:5:5:10:

10. The weight ratio of calcium lactate, sodium chloride, potassium chloride, sodium dihydrogen phosphate, magnesium sulfate, zinc sulfate, manganese sulfate, ferrous sulfate and selenium yeast powder in the multidimensional element powder is 50:10:5:8:3:6:5:6:

7. The weight ratio of lauric acid, linolenic acid and shikimic acid in the organic acids is 24:36:

40.

8. A feed for feeding stink bugs, characterized in that, It is prepared using the nine-fragrant insect feeding composition according to any one of claims 1-7 as raw material.

9. A method for preparing stink bug feed using the stink bug feeding composition according to any one of claims 1-7, characterized in that, Includes the following steps: The product is obtained by mixing Cucurbitaceae plant extracts, compound amino acid powder, glucose powder, organic acids, and multi-dimensional element powder.

10. The use of the stink bug feeding composition according to any one of claims 1-7, or the stink bug feeding feed according to claim 8, or the stink bug feeding feed prepared by the method according to claim 9, in the production of insect feed products.

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