Method for simultaneously extracting panax japonicus saponin and polysaccharide, panax japonicus saponin polysaccharide extract, cream and cream preparation method
The saponins and polysaccharides of *Panax notoginseng* were extracted simultaneously using an ethanol-water solution via ultrasonic or cell wall disruption methods. The resulting saponin and polysaccharide extract was used for skin care, solving the problem of complex extraction methods in existing technologies and achieving highly effective antioxidant effects and delaying skin aging.
Patent Information
- Application Number
- CN202511107919.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-08-08
- Publication Date
- 2025-11-11
AI Technical Summary
In existing technologies, the extraction methods for ginseng saponins and polysaccharides are carried out separately, which is complicated and inefficient, making it difficult to achieve the combined or synergistic effects of the two in clinical practice, and has not been effectively applied to delay skin aging.
Using an aqueous ethanol solution as a solvent, saponins and polysaccharides of Panax notoginseng were extracted simultaneously by ultrasonic or cell wall disruption methods. Subsequently, Panax notoginseng saponin and polysaccharide extracts were prepared and applied to creams that delay skin aging.
The simultaneous and efficient extraction of ginseng saponins and polysaccharides was achieved. The extract has significant antioxidant capacity, can effectively scavenge free radicals, delay skin aging, and the prepared cream has obvious antioxidant effects.
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Figure CN120919185A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the fields of medicine and cosmetics, specifically to a method for simultaneously extracting ginseng saponins and polysaccharides, as well as ginseng saponin and polysaccharide extracts, creams, and cream preparation methods. Background Technology
[0002] Panax japonicus CAMey., a plant belonging to the genus Panax in the family Araliaceae, is widely distributed in Hubei, Yunnan, Guizhou and other regions of my country. It has a complex chemical composition and significant biological activity.
[0003] The main chemical components of *Panax japonicus* are saponins, polysaccharides, amino acids, volatile oils, and inorganic salts.
[0004] Saponins from *Panax japonicus* are the most important bioactive components of this ginseng. They are almost insoluble in water. Currently, the main extraction method for saponins is high-concentration alcohol extraction, while the main extraction method for polysaccharides is water extraction followed by alcohol precipitation. The separate extraction of saponins and polysaccharides is complex, but the various clinical effects are attributed to the combined or synergistic effects of both types of components. Summary of the Invention
[0005] This invention aims to solve the problems of separate extraction and combined application of two types of components with clinical common or synergistic effects in the existing technology, which are complicated and inefficient. The first objective of this invention is to provide a method for simultaneously extracting ginseng saponins and polysaccharides. The second objective is to provide a ginseng saponin and polysaccharide extract obtained by this method. The third objective is to provide the application of the ginseng saponin and polysaccharide extract in delaying skin aging. The fourth objective is to provide a cream for delaying skin aging. The fifth objective is to provide a method for preparing the cream.
[0006] To achieve the aforementioned first objective, the present invention provides a method for simultaneously extracting saponins and polysaccharides from Panax notoginseng, characterized by the following extraction method: Panax notoginseng powder is added to a solvent at a ratio of 1:15, and the mixture is subjected to cell wall disruption in a cell wall disruptor for 30 seconds, followed by ultrasonic extraction at room temperature for 30 minutes at an ultrasonic power of 120W. The solvent is an aqueous ethanol solution with a volume concentration of 15%-75%.
[0007] Alternatively, the powder of *Panax notoginseng* can be added to a solvent for ultrasonic extraction at a ratio of 1:15, at room temperature, twice for 30 minutes each time, with an ultrasonic power of 120W. The solvent is an aqueous ethanol solution with a volume concentration of 15%-75%.
[0008] In the above scheme, the volume concentration of the ethanol aqueous solution is 15%.
[0009] The second objective of this invention is achieved as follows: a *Panax japonicus* saponin polysaccharide extract, characterized in that: after the extraction of *Panax japonicus* saponins and polysaccharides is completed by the method of simultaneously extracting *Panax japonicus* saponins, the extract is filtered, concentrated, and the solvent is removed to obtain the *Panax japonicus* saponin polysaccharide extract.
[0010] The application of the aforementioned bamboo joint ginseng saponin polysaccharide extract in delaying skin aging.
[0011] Another object of the present invention is achieved as follows: a cream for delaying skin aging, characterized in that it contains the aforementioned ginseng saponin polysaccharide extract.
[0012] The above formula is made from the following raw materials in weight percentage: 0.1-10% *Panax japonicus* saponin polysaccharide extract, 0.5-3% ethanol aqueous solution, 2-4% glyceryl monostearate, 4-6% Tween 80, 2-25% shea butter, 0.2-8% cetearyl alcohol, 1.5-15% olive oil, 1.5-15% glycerin, 0.4-2.5% sodium lauryl sulfate, 1-1.4% triethanolamine, 0.08-0.15% ethylparaben, with the balance being water. The volume concentration of ethanol in the ethanol aqueous solution is 15%.
[0013] A method for preparing the aforementioned anti-aging cream is characterized by the following steps: Dissolving *Gynostemma pentaphyllum* saponin polysaccharide extract in an ethanol-water solution; heating and melting glyceryl monostearate, Tween, shea butter, cetearyl alcohol, olive oil, and ethylparaben to form an oil phase and maintaining the temperature at 85-95°C; dissolving glycerin, sodium lauryl sulfate, and triethanolamine in water to form an aqueous phase; slowly adding the aqueous phase to the oil phase in a water bath at 80-90°C; stirring and emulsifying until homogeneous; then adding the ethanol solution of *Gynostemma pentaphyllum* saponin polysaccharide extract; homogenizing and emulsifying for 5-20 minutes; cooling in a cold water bath to 40-45°C; and then naturally cooling to room temperature to obtain the product.
[0014] Beneficial effects: The extraction method of this invention is simple and can simultaneously extract ginseng saponins and polysaccharides. The extract has significant antioxidant capacity, with a free radical scavenging rate of 96.4%, and can be made into cream-type skin care products with antioxidant effects. Attached Figure Description
[0015] Figure 1 The standard curve for the total saponin content of Panax notoginseng.
[0016] Figure 2 The standard curve for the polysaccharide content of Panax notoginseng.
[0017] Figure 3 This is a photograph of the cream prepared according to the present invention.
[0018] Figure 4 This is the DPPH standard curve.
[0019] Figure 5 The DPPH scavenging rate of different concentrations of vitamin E.
[0020] Figure 6 The DPPH scavenging rate of different concentrations of Panax notoginseng extract. Detailed Implementation
[0021] The present invention will be further illustrated by the following examples:
[0022] Example 1
[0023] 1. Establishment of a method for determining the total saponin content of Panax notoginseng
[0024] 1.1 Selection of detection wavelength
[0025] Accurately transfer 0.2 mL each of the ginsenoside Ro standard solution (purchased from Shanghai Yuanye Biotechnology Co., Ltd.) and the ginsenoside Ro test solution into 5 mL volumetric flasks. After evaporating the solvent in a 66℃ water bath, add 0.2 mL of freshly prepared 0.5% vanillin-glacial acetic acid colorimetric reagent and 0.8 mL of perchloric acid solution sequentially. React in a 66℃ constant temperature water bath for 20 min, then immediately cool in an ice-water bath for 10 min. Finally, dilute to the mark with glacial acetic acid, shake thoroughly, and let stand for 15 min. For the blank control solution, use 0.2 mL of methanol instead of the standard or test solution, and operate in the same manner. Use a UV-Vis spectrophotometer to scan the absorption spectrum of ginsenoside Ro in the wavelength range of 400-800 nm and record its maximum absorption wavelength. The experimental results show a characteristic absorption peak near 551 nm. Based on the literature, 551 nm was finally determined as the detection wavelength for the determination of total saponin content.
[0026] 1.2 Preparation of Standard Curve
[0027] Accurately pipette 0.2 mL, 0.4 mL, 0.6 mL, 1.0 mL, and 2.0 mL of ginsenoside Ro reference solution (Shanghai Yuanye Biotechnology Co., Ltd. and Nanjing Yuanzhi Biotechnology Co., Ltd.) and dilute to volume to obtain standard solutions of different concentrations (see [link to standard solution]). Figure 1 The absorbance of each sample was determined according to the method described under "Detection Wavelength," and a standard curve was plotted using absorbance A and reference concentration C (mg / mL). The regression equation for ginsenoside Ro reference standard was y = 0.2556x + 0.0272 R. 2 =0.9973, see standard curve. Figure 1 .
[0028] 1.3 Precision Test
[0029] Five 0.2 mL aliquots of ginsenoside Ro reference solution (Shanghai Yuanye Biotechnology Co., Ltd.) were precisely pipetted, and the absorbance of each sample was measured according to the method under "Detection Wavelength" and the RSD value was calculated. The result of RSD = 0.4% indicates that the instrument has good precision.
[0030] 1.4 Stability Test
[0031] The absorbance of the intermediate concentration of ginsenoside Ro reference solution under the "Standard Curve" section was measured at 0, 20, 40, 60, 80, and 120 min, and the RSD value was calculated. The RSD was 1.6%, indicating that the reference solution had good stability within 2 h.
[0032] 1.5 Repeatability Test
[0033] Five 0.2 mL aliquots of the total saponin solution from Panax notoginseng were precisely pipetted, and the absorbance was measured according to the method under "Detection Wavelength" and the RSD value was calculated. The RSD was 5.5%, indicating that the method has good repeatability.
[0034] 1.6 Recovery Experiment
[0035] Five 10 μL portions of the extract containing 2.858 mg / mL total saponins from Panax notoginseng were accurately measured. Different amounts of standard solution were added to each portion, and the absorbance was measured according to the method under "Detection Wavelength". The absorbance was then substituted into the standard curve equation to calculate the recovery rate and RSD of total saponins from Panax notoginseng. The average recovery rate of total saponins from Panax notoginseng was 101.03%, and the RSD was 2.57%.
[0036] Table 1 Results of the recovery rate test of total saponins from Panax notoginseng.
[0037]
[0038]
[0039] 2. Establishment of a method for determining the polysaccharide content of Panax notoginseng
[0040] 2.1 Preparation of glucose reference solution
[0041] Table 2 Preparation of glucose standard solution
[0042]
[0043] 2.2 Selection of detection wavelength
[0044] Accurately pipette 2 ml of glucose reference solution and *Gynostemma pentaphyllum* polysaccharide test solution into 10 ml stoppered test tubes. Add 5 ml of freshly prepared 2 g / L scallion-80% sulfuric acid solution to each tube, shake well, and heat in a 100°C water bath for 10 min in the dark. Remove and quickly cool in an ice-water bath to room temperature. Prepare the blank control solution by replacing the glucose reference solution or test solution with 2 ml of purified water, following the same procedure. Scan the wavelength range of 400-800 nm using a UV-Vis spectrophotometer. Read the maximum absorption wavelength of the reference and test solutions. The experimental results show that the glucose and *Gynostemma pentaphyllum* polysaccharide solutions exhibit a maximum absorption peak near 628 nm. Based on literature review, 628 nm was ultimately determined as the detection wavelength for determining the content of *Gynostemma pentaphyllum* polysaccharide.
[0045] 2.3 Preparation of Standard Curve
[0046] Accurately pipette 0, 0.1, 0.2, 0.4, 0.5, and 0.6 mL of 0.1 mg / mL glucose reference solution into 10 mL stoppered test tubes, respectively, and add water to a final volume of 1 mL. Prepare and measure the absorbance of each sample according to the method described under the "Detection Wavelength" section for polysaccharides. Establish a regression equation using absorbance A and reference concentration C. The regression equation for glucose reference is y = 42.964x + 0.0345, R0. 2 =0.9979. The standard working curve is as follows: Figure 2 As shown.
[0047] 2.4 Precision Test
[0048] Accurately pipette 0.4 mL of 0.1 mg / mL glucose reference solution into a 5 mL volumetric flask. Measure the absorbance of the sample solution six times consecutively at 628 nm using methanol as a blank solvent, following the method described under the "Detection Wavelength" section for polysaccharides. Calculate the RSD value. The RSD = 0.28%, indicating good instrument precision.
[0049] 2.5 Stability Test
[0050] A glucose reference solution with an intermediate concentration under the standard curve was taken, and the absorbance was measured at 628 nm with methanol as the blank solvent at 0, 20, 40, 60, 80 and 120 min, and the RSD value was calculated. The RSD = 2.2%, which shows that the reference solution has good stability within 2 h.
[0051] 2.6 Repeatability Experiment
[0052] Five 0.4 mL aliquots of 0.1 mg / mL glucose reference solution were precisely pipetted into 10 mL stoppered test tubes. The absorbance was prepared and measured according to the method under the "Detection Wavelength" section of the polysaccharide section. The RSD value was calculated. The RSD value was 1.8%, indicating that the method has good repeatability.
[0053] 2.7 Recovery Experiment
[0054] Five 10 μL portions of extract containing 2.015 mg / mL of *Panax notoginseng* polysaccharide were precisely measured and added to unequal amounts of reference solution. The absorbance was prepared and measured according to the method under the "Detection Wavelength" section for polysaccharides. The results were substituted into the standard curve equation to calculate the recovery rate and RSD of *Panax notoginseng* polysaccharide. The average recovery rate of *Panax notoginseng* polysaccharide was 101.93%, and the RSD was 2.89%.
[0055] Table 3. Experimental Investigation of the Recovery Rate of Polysaccharide from Ginseng (Panax notoginseng)
[0056]
[0057] 3. Examination of extraction methods
[0058] (1) A method for simultaneously extracting saponins and polysaccharides from *Panax notoginseng*, comprising the following extraction method: *Panax notoginseng* powder is added to a solvent and ultrasonically extracted at a ratio of 1:15 at room temperature. The extraction is performed twice (after the first extraction, the mixture is filtered, and the residue is extracted again), each extraction lasting 30 minutes. The ultrasonic power is 120 W, and the solvent is an aqueous ethanol solution with volume concentrations of 5%, 15%, 30%, 45%, and 75% respectively. After extraction, the mixture is filtered, the extracts are combined, and the solvent is concentrated to obtain a thick extract of *Panax notoginseng* saponins and polysaccharides.
[0059] (2) Add the bamboo joint ginseng powder to the solvent at a ratio of 1:15, and break it in a high-speed blender for 30 seconds. Then, at room temperature, perform ultrasonic extraction for 30 minutes at an ultrasonic power of 120W. The solvent is an aqueous ethanol solution, and the extraction is carried out at 45% and 15% ethanol volume concentration, respectively. After extraction, filter the solution and concentrate the filtrate to obtain a thick extract.
[0060] (3) Add the *Panax notoginseng* powder to the solvent at a ratio of 1:15, add ethanol solution, and reflux for 2 hours. Reflux extraction was performed at ethanol concentrations of 45% and 15% (v / v). After extraction, the mixture was filtered, and the filtrate was concentrated to obtain a thick extract. The extraction results are shown in Table 4 below:
[0061] Table 4 Extraction Results
[0062]
[0063] As can be seen from Table 4, the best results were obtained by combining 15% ethanol for 30 seconds of cell wall disruption with ultrasound for 30 minutes, and ultrasound for 30 minutes twice, which can simultaneously extract total saponins and polysaccharides.
[0064] Test case
[0065] Determination of the DPPH free radical scavenging ability of ginseng saponin polysaccharide extract
[0066] DPPH (1,2-diphenyl-2-picrylhydrazine), as a stable nitrogen-centered free radical, possesses unpaired electrons in its molecular structure, thus enabling it to accept one electron or a hydrogen ion. DPPH is stable in organic solvents, and its alcoholic solution is purple, exhibiting maximum absorption at 517 nm. In the presence of free radical scavengers, the unpaired electrons of DPPH are captured, causing its color to lighten and the absorbance at the maximum absorption wavelength to decrease linearly. This decrease in absorbance indicates an increase in antioxidant activity, thus evaluating the antioxidant capacity of the test sample. Therefore, this experiment assessed the antioxidant activity of *Panax notoginseng* saponin polysaccharide extract by measuring the change in absorbance at 517 nm before and after the reaction of DPPH solution with *Panax notoginseng* extract solution. Vitamin E was used as a positive control. The scavenging effects of *Panax notoginseng* total extract (15% ethanol reflux; a mixture of extracts obtained by two separate processes of cell disruption followed by 30 seconds of sonication followed by 30 minutes of sonication) and vitamin E on DPPH free radicals were systematically investigated and compared.
[0067] Preparation of DPPH stock solution: Accurately weigh 2.6 mg of DPPH powder, place it in a 100 mL volumetric flask, add 95% (v / v) ethanol to dissolve and dilute to the mark, mix thoroughly to prepare a 26.0 mg / L stock solution.
[0068] 1. Establishment of the standard working curve: Accurately pipette 1.0 mL, 2.0 mL, 4.0 mL, 6.0 mL, 8.0 mL, and 10.0 mL of the above stock solution into 10 mL volumetric flasks, dilute to the mark with 95% ethanol, and mix well. Measure the absorbance of each solution at 517 nm using a UV-Vis spectrophotometer. Plot a standard curve using absorbance A and DPPH concentration C (mg / L). Figure 4 Then, linear regression was performed, yielding the linear regression equation y = 0.023x + 0.004, R0. 2 =0.9999.
[0069] like Figure 5 and 6 As shown in the results, the test results indicate that even after dilution, the *Panax notoginseng* saponin polysaccharide extract still exhibits excellent scavenging ability against DPPH free radicals. Specifically, the *Panax notoginseng* extract at different concentrations of dilution showed better DPPH free radical scavenging effect than vitamin E. After comparing the scavenging rates at different dilution ratios, the results showed that the *Panax notoginseng* extract at a concentration of 0.2766 mg / mL had the most ideal DPPH free radical scavenging effect.
[0070] Scavenging rate E = (A0 - A) / A0 * 100%. (A0 is the absorbance of the DPPH standard solution, and A is the absorbance after adding the sample or the positive control).
[0071] Free radicals damage skin cell structure and interfere with normal function, leading to skin oxidation and aging. The total saponins and polysaccharides in *Panax japonicus* have significant antioxidant capabilities, effectively scavenging superoxide anion free radicals and hydroxyl free radicals in the body. This reduces oxidative damage to skin cells, thereby slowing down the skin aging process.
[0072] Example 2
[0073] This anti-aging cream is made from the following raw materials in weight percentages: 0.1% *Panax japonicus* saponin polysaccharide extract, 0.5% aqueous ethanol solution, 2% glyceryl monostearate, 4% Tween 80, 2% shea butter, 8% cetearyl alcohol, 1.5% olive oil, 12% glycerin, 0.4% sodium lauryl sulfate, 1% triethanolamine, 0.08% ethylparaben, and the balance being water. The volume concentration of ethanol in the aqueous ethanol solution is 15%.
[0074] Dissolve the saponin polysaccharide extract of *Gynostemma pentaphyllum* in an ethanol-water solution. Melt glyceryl monostearate, Tween, shea butter, cetearyl alcohol, olive oil, and ethylparaben into an oil phase and keep warm at 85-95℃. Dissolve glycerol, sodium lauryl sulfate, and triethanolamine in water in a water bath at 80-90℃ to form an aqueous phase and keep warm. Slowly add the aqueous phase to the oil phase and stir until emulsified evenly. Then add the ethanol solution of the saponin polysaccharide extract of *Gynostemma pentaphyllum*, homogenize and emulsify for 5-20 minutes, cool in a cold water bath to 40-45℃, and then allow to cool naturally to room temperature to obtain the product.
[0075] Example 3
[0076] This anti-aging cream is made from the following raw materials in weight percentages: 5% *Panax japonicus* saponin polysaccharide extract, 1.5% aqueous ethanol solution, 4% glyceryl monostearate, 6% Tween 80, 25% shea butter, 0.2% cetearyl alcohol, 15% olive oil, 15% glycerin, 2.5% sodium lauryl sulfate, 1.4% triethanolamine, 0.15% ethylparaben, and the balance being water. The volume concentration of ethanol in the aqueous ethanol solution is 15%.
[0077] Dissolve the saponin polysaccharide extract of *Gynostemma pentaphyllum* in an ethanol-water solution. Melt glyceryl monostearate, Tween, shea butter, cetearyl alcohol, olive oil, and ethylparaben into an oil phase and keep warm at 85-95℃. Dissolve glycerol, sodium dodecyl sulfate, and triethanolamine in water in a water bath at 80-90℃ to form an aqueous phase and keep warm. Slowly add the aqueous phase to the oil phase while stirring and emulsifying evenly. Then add the ethanol solution of the saponin polysaccharide extract of *Gynostemma pentaphyllum* and homogenize and emulsify for 5-20 minutes. Cool to 40-45℃ in a cold water bath and then allow to cool naturally to room temperature to obtain the product.
[0078] Example 4
[0079] This anti-aging cream is made from the following raw materials in weight percentages: 10% *Panax japonicus* saponin polysaccharide extract, 3% aqueous ethanol solution, 3% glyceryl monostearate, 5% Tween 80, 10% shea butter, 2% cetearyl alcohol, 1.5% olive oil, 10% glycerin, 1% sodium lauryl sulfate, 1.2% triethanolamine, 0.1% ethylparaben, and the balance being water. The volume concentration of ethanol in the aqueous ethanol solution is 15%.
[0080] Dissolve the saponin polysaccharide extract of *Gynostemma pentaphyllum* in an ethanol-water solution. Melt glyceryl monostearate, Tween, shea butter, cetearyl alcohol, olive oil, and ethylparaben into an oil phase and keep warm at 85-95℃. Dissolve glycerol, sodium lauryl sulfate, and triethanolamine in water in a water bath at 80-90℃ to form an aqueous phase and keep warm. Slowly add the aqueous phase to the oil phase and stir until emulsified evenly. Then add the ethanol solution of the saponin polysaccharide extract of *Gynostemma pentaphyllum*, homogenize and emulsify for 5-20 minutes, cool in a cold water bath to 40-45℃, and then allow to cool naturally to room temperature to obtain the product.
[0081] Test case
[0082] Extract cream has the effect of delaying skin aging.
[0083] 1. Experimental Methods: 12-30 volunteers with healthy skin and no allergic diseases were selected. The test products and control products from Examples 2-4 were randomly applied to different sides of the hand or face. Before use, and at 5 min, 10 min, 30 min, 1 h, 2 h, and 4 h after use, the improvement in skin moisture, oil content, and elasticity was observed using the Real Bubee Smart Display Skin Analyzer. Before use, and at 2 weeks and 4 weeks after use, the Visible Analysis (VISIA) test was performed. The System skin test analyzer observes the improvement of nine indicators: pores, pigmentation, wrinkles, roughness, black and white areas, pigmentation, skin moisture, oil content, and elasticity. It combines skin feel and observation to score the product using a scale. The product's appearance, texture, smell, and user experience are subjectively or semi-quantitatively analyzed through human senses (sight, touch, smell, etc.). The product's color uniformity, gloss, transparency, spreadability, viscosity, residue, application resistance, and absorption are comprehensively scored for the Bamboo Ginseng Total Extract Skin Anti-Aging Cream.
[0084] The specific steps are as follows:
[0085] Sample application: From the start of the test, subjects should first cleanse the selected test area (facial or hand skin), then sit quietly in the designated test environment for at least 15 minutes. During this time, the test area must be fully exposed, and subjects should remain relaxed and avoid touching or interfering with the test area. For test site selection, the backs of the left and right hands, or the left and right cheeks, are used. Sample application areas and control areas are determined through random grouping (the blank control is a blank cream prepared without the addition of *Panax notoginseng* saponin polysaccharide extract).
[0086] Skin index determination: At least three parallel measurements were performed on all test areas, and the average value was calculated to improve data reliability. The probe was cleaned before each measurement to avoid cross-contamination. First, the initial values were measured before use; then, the sample and control were evenly applied to the skin area to be tested; the skin condition of the sample area and the control area were measured at the set time points.
[0087] In a 4-week human safety and efficacy evaluation experiment, none of the 12 healthy adult subjects experienced acute skin allergic reactions such as erythema, edema, or pigmentation, indicating that the product is safe and caused no adverse skin reactions. Dynamic monitoring of the subjects' hand and facial skin was conducted using the VISIA skin test analyzer and the RealBubee intelligent display skin tester. Results showed:
[0088] Immediate moisturizing effect: The moisture content of the stratum corneum of the skin on the hands increased significantly after the subjects used the product.
[0089] Sebum regulation ability: After 4 hours of product use, the surface oil content of the skin on the hands of the subjects increased slightly from 46.71±4.11% to 48.71±2.81%, confirming that the product has bidirectional sebum regulation properties.
[0090] Elasticity improvement effect: After 4 hours of product use, the elasticity of the subjects' skin increased from 35.00±12.06% to 43.28±8.34%, indicating that the product can promote the reconstruction of collagen fiber network.
[0091] After two weeks of continuous use by the subjects:
[0092] The stratum corneum moisture content on the cheeks increased by 20% compared to the initial value. The VISIA facial image analysis system showed a 22.95% reduction in skin texture density, a 7.65% reduction in pore area ratio, and a 14.1% increase in brightness value in pigmented areas, confirming that the product has significant moisturizing, wrinkle reduction, pore shrinking, and pigmentation improvement effects.
[0093] When the usage period is extended to 4 weeks, the skin improvement effect is further enhanced:
[0094] The stratum corneum moisture content of the cheeks increased by 37.1% compared to the initial level; skin roughness decreased from 47.54% to 34.06%; wrinkle depth decreased by 23.92%; number of pores decreased by 9.32%; and the area of pigmentation decreased by 18.05%, indicating that the product's moisturizing, wrinkle-reducing, pore-shrinking, and pigmentation-improving effects become more significant with prolonged use.
[0095] Table 5. Results of Skin Care Performance Test
[0096]
[0097]
[0098] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.
Claims
1. A method for simultaneously extracting saponins and polysaccharides from Panax notoginseng, characterized in that, Extraction was performed as follows: Powdered Codonopsis pilosula was added to a solvent at a ratio of 1:15, and the mixture was processed in a high-speed blender for 30 seconds. Then, it was ultrasonically extracted at room temperature for 30 minutes with an ultrasonic power of 120W. The solvent was an aqueous ethanol solution with a volume concentration of 15%-75%. Alternatively, add the powdered Chinese ginseng to a solvent at a ratio of 1:15, and extract twice at room temperature using ultrasound, each time for 30 minutes, with an ultrasound power of 120W. The solvent is an aqueous ethanol solution with a volume concentration of 15%-75%.
2. The method for simultaneously extracting saponins and polysaccharides from *Panax japonicus* according to claim 1, characterized in that: The volume concentration of the ethanol aqueous solution is 15%.
3. A saponin polysaccharide extract from *Panax japonicus*, characterized in that: After extraction according to any one of claims 1-2, the method for simultaneously extracting saponins and polysaccharides from *Panax japonicus* is completed, followed by filtration and concentration to remove the solvent, resulting in a saponin and polysaccharide extract from *Panax japonicus*.
4. The application of the bamboo joint ginseng saponin polysaccharide extract according to claim 3 in delaying skin aging.
5. A cream for delaying skin aging, characterized in that: It contains the bamboo joint ginseng saponin polysaccharide extract as described in claim 3.
6. The cream for delaying skin aging according to claim 5, characterized in that: It is made from the following raw materials in weight percentage: 0.1-10% of *Panax japonicus* saponin polysaccharide extract, 0.5-3% of aqueous ethanol solution, 2-4% of glyceryl monostearate, 4-6% of Tween 80, 2-25% of shea butter, 0.2-8% of cetearyl alcohol, 1.5-15% of olive oil, 1.5-15% of glycerin, 0.4-2.5% of sodium lauryl sulfate, 1-1.4% of triethanolamine, 0.08-0.15% of ethylparaben, with the balance being water, wherein the volume concentration of ethanol in the aqueous ethanol solution is 15%.
7. A method for preparing a cream for delaying skin aging as described in claim 6, characterized in that, The preparation method is as follows: Dissolve the saponin polysaccharide extract of *Gynostemma pentaphyllum* in an ethanol-water solution. Melt glyceryl monostearate, Tween, shea butter, cetearyl alcohol, olive oil, and ethylparaben to form the oil phase and keep it at 85-95℃. Dissolve glycerol, sodium dodecyl sulfate, and triethanolamine in water to form the aqueous phase. Slowly add the aqueous phase to the oil phase in a water bath at 80-90℃. After stirring and emulsifying evenly, add the ethanol solution of the saponin polysaccharide extract of *Gynostemma pentaphyllum*, homogenize and emulsify for 5-20 minutes, cool in a cold water bath to 40-45℃, and then cool naturally to room temperature to obtain the product.