Anti-oxidation, whitening and brightening composition as well as preparation method and application thereof

Abstract

The invention discloses an anti-oxidation, whitening and brightening composition as well as a preparation method and application thereof. The anti-oxidation, whitening and brightening composition is prepared from the following components in parts by weight: 2 to 6 parts of nicotinamide, 0.5 to 5 parts of tranexamic acid, 1 to 5 parts of nonapeptide-1 solution, 0.5 to 1 part of tetrahydromethylpyrimidine carboxylic acid, 0.5 to 2 parts of pink red plum compound essence, 0.01 to 0.5 part of hydroxyethyl piperazine ethanesulfonic acid, 0.01 to 0.5 part of glabridin and 0.0001 to 0.2 part of glutathione. According to the invention, the composition prepared from nicotinamide, tranexamic acid, nonapeptide-1, tetrahydromethylpyrimidine carboxylic acid, pink-red plum composite essence, hydroxyethyl piperazine ethanesulfonic acid, glabridin and glutathione is prepared into the essence, and the clearance rate of the essence to ABTS free radicals reaches 58% or above, which shows that the essence has very good antioxidant ability; the cell tyrosinase activity inhibition rate of the composition under the concentration of 1.2% (v / v) reaches 51% or above, which shows that the composition has very good whitening and brightening effects.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of cosmetic technology, in particular to an antioxidant and whitening composition, a preparation method and application thereof. BACKGROUND

[0002] Skin aging is a comprehensive result of free radical damage, mainly manifested as dry, rough, wrinkled, pale and dull, loose and inelastic skin, and even skin atrophy, cracking and age spots. Vitamins and their derivatives have good free radical scavenging effect, but due to their poor stability, they are prone to discoloration and inactivation in actual application, and it is difficult to meet the continuous antioxidant demand.

[0003] Skin whitening is mainly related to melanin production inhibition activity and free radical scavenging ability. Tyrosinase is the main rate-limiting enzyme for melanin synthesis, and its activity determines the amount of melanin formation. Oxidative damage induced by oxygen free radicals and enhanced tyrosinase activity induce skin melanin increase.

[0004] It has a very broad application prospect to develop cosmetics with antioxidant and whitening effects to meet the needs of consumers. SUMMARY

[0005] In view of the deficiencies of the prior art, the purpose of the present application is to provide an antioxidant and whitening composition, a preparation method and application thereof.

[0006] The purpose of the present application is achieved by the following technical solutions: In a first aspect, the present application provides an antioxidant and whitening composition, comprising the following components by weight: nicotinamide 2-6 parts, tranexamic acid 0.5-5 parts, nonapeptide-1 solution 1-5 parts, tetrahydro methyl pyrimidine carboxylic acid 0.5-1 part, pine red plum complex extract 0.5-2 parts, hydroxyethyl piperazine ethane sulfonic acid 0.01-0.5 parts, glabridin 0.01-0.5 parts, glutathione 0.0001-0.2 parts.

[0007] Preferably, the weight ratio of the nonapeptide-1 solution, the pine red plum complex extract and the glabridin is 2-3:1-2:0.1-0.3.

[0008] In a second aspect, the present application provides the use of an antioxidant and whitening composition in the preparation of cosmetics.

[0009] According to claim 3, the dosage form of the cosmetic is selected from any one of the following: cream, emulsion, water.

[0010] In a third aspect, the present application provides a serum comprising the aforementioned antioxidant and whitening composition, and further comprising at least one of a humectant, an antioxidant, a thickening agent, a pH adjusting agent, a chelating agent, a skin conditioning agent, and water.

[0011] Preferably, the serum comprises the following components in the following weight proportions: nicotinamide 2-6 parts, tranexamic acid 1-4 parts, nonapeptide-1 solution 1-5 parts, tetrahydro methyl pyrimidine carboxylic acid 0.5-1 part, pine red plum complex extract 0.5-2 parts, hydroxyethyl piperazine ethane sulfonic acid 0.01-0.5 parts, glabridin 0.01-0.5 parts, glutathione 0.0001-0.2 parts, humectant 4-14 parts, antioxidant 0.05-0.8 parts, thickening agent 0.1-0.5 parts, pH adjusting agent 0.1-0.5 parts, chelating agent 0.01-0.5 parts, skin conditioning agent 0.1-0.3 parts, and water to make up to 100 parts.

[0012] Preferably, the humectant is selected from at least one of butylene glycol, panthenol, 1,2-hexanediol, and sodium hyaluronate.

[0013] Preferably, the antioxidant is selected from at least one of p-hydroxyacetophenone and sodium metabisulfite.

[0014] Preferably, the thickening agent is selected from acrylates / C10-30 alkyl acrylate crosspolymer.

[0015] Preferably, the pH adjusting agent is selected from tromethamine.

[0016] Preferably, the skin conditioning agent is selected from allantoin.

[0017] Preferably, the chelating agent is selected from disodium EDTA.

[0018] Preferably, the serum comprises the following components in the following weight proportions: nicotinamide 2-6 parts, tranexamic acid 1-4 parts, nonapeptide-1 solution 1-5 parts, tetrahydro methyl pyrimidine carboxylic acid 0.5-1 part, pine red plum complex extract 0.5-2 parts, hydroxyethyl piperazine ethane sulfonic acid 0.01-0.5 parts, glabridin 0.01-0.5 parts, glutathione 0.0001-0.2 parts, butylene glycol 3-10 parts, panthenol 0.5-1 part, 1,2-hexanediol 0.5-3 parts, sodium hyaluronate 0.01-0.1 part, p-hydroxyacetophenone 0.01-0.5 part, sodium metabisulfite 0.03-0.1 part, acrylates / C10-30 alkyl acrylate crosspolymer 0.1-0.5 part, tromethamine 0.1-0.5 part, disodium EDTA 0.01-0.5 part, allantoin 0.1-0.3 part, and water to make up to 100 parts.

[0019] Fourthly, the present invention provides a method for preparing the aforementioned serum, characterized by comprising the following steps: weighing each component according to the weight ratio, mixing and stirring evenly to obtain the serum.

[0020] Compared with the prior art, the present invention has the following beneficial effects: This invention utilizes a composition prepared from nicotinamide, tranexamic acid, nonapeptide-1, tetrahydromethylpyrimidine carboxylic acid, pine saffron complex extract, hydroxyethylpiperazine ethanesulfonic acid, glycyrrhizin, and glutathione. When this composition is formulated into an essence, its scavenging rate against ABTS free radicals reaches over 58%, indicating excellent antioxidant capacity. Furthermore, at a concentration of 1.2% (v / v), its inhibition rate against cellular tyrosinase activity reaches over 51%, demonstrating excellent whitening and brightening effects. Detailed Implementation

[0021] The present invention will now be described in detail with reference to specific embodiments. These embodiments will help those skilled in the art to further understand the present invention, but do not limit the invention in any way. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention. These all fall within the scope of protection of the present invention.

[0022] The terms "preferred," "more preferably," and "more suitable" used in this invention refer to embodiments of the invention that provide certain beneficial effects under certain circumstances. However, other embodiments may also be preferred under the same or other circumstances. Furthermore, the description of one or more preferred embodiments does not imply that other embodiments are unavailable, nor is it intended to exclude other embodiments from the scope of this invention.

[0023] It should be understood that, except in any operational instance or otherwise indicated, all figures representing the amounts of ingredients used, for example, in the specification and claims, should be understood to be modified in all cases by the term "about". Therefore, unless otherwise stated, the numerical parameters set forth in the following specification and appended claims are approximations varying with the desired performance to be obtained according to the invention. It is not at all an attempt to limit the application of the doctrine of equivalents to the scope of the claims; each numerical parameter should be interpreted at least according to the number of significant figures reported and by applying ordinary rounding techniques.

[0024] Although the numerical ranges and parameters illustrating the broad scope of the invention are approximate, the values ​​listed in the specific examples are reported as precisely as possible. However, any numerical value inherently contains some error that is necessarily caused by the standard deviation found in their respective test measurements.

[0025] The following examples provide an antioxidant and whitening composition comprising the following components in parts by weight: 2-6 parts niacinamide, 0.5-5 parts tranexamic acid, 1-5 parts nonapeptide-1 solution, 0.5-1 part tetrahydromethylpyrimidine carboxylic acid, 0.5-2 parts pine saffron compound extract, 0.01-0.5 parts hydroxyethylpiperazine ethanesulfonic acid, 0.01-0.5 parts glycyrrhizin, and 0.0001-0.2 parts glutathione.

[0026] In one specific embodiment, the weight ratio of the nonapeptide-1 solution, pine saffron compound extract, and glycyrrhizin is 2-3:1-2:0.1-0.3. This ratio can further enhance the whitening and brightening effects.

[0027] In one specific embodiment, the nicotinamide used was purchased from Hangzhou Weibaolai Biotechnology Co., Ltd., and its trade name is nicotinamide. It should be noted that the nicotinamide used in this invention can also be from corresponding products of other companies, and this invention does not impose any particular limitation.

[0028] In one specific embodiment, the tranexamic acid is purchased from MARUZEN PHARMACEUTICALS CO.,LTD., under the trade name Tranexamic Acid NK. A more preferred content of tranexamic acid is 1 to 4 parts. It should be noted that the tranexamic acid used in this invention can also be from corresponding products of other companies, and this invention does not impose any particular limitation.

[0029] In one specific embodiment, the nonapeptide-1 solution was purchased from Dongguan Weiqi Technology Co., Ltd., under the trade name WKPep® PCrystide Plus. The weight parts of the nonapeptide-1 solution are the same as the weight parts of the product WKPep® PCrystide Plus. The product's components are glycerin, water, and nonapeptide-1 in a mass ratio of 80:19.95:0.05. It should be noted that the nonapeptide-1 solution used in this invention can also be from other companies' corresponding products; this invention does not impose any particular limitation.

[0030] In one specific embodiment, the tetrahydromethylpyrimidine carboxylic acid was purchased from Zhejiang Huarui Biotechnology Co., Ltd., under the trade name Ectoine. It should be noted that the tetrahydromethylpyrimidine carboxylic acid used in this invention can also be from corresponding products of other companies; this invention does not impose any particular limitation.

[0031] In one specific embodiment, the pine-red-plum compound extract was purchased from Guangzhou GANENG Biotechnology Co., Ltd., and its trade name is EneSoothe-MIX-ManukaJN1227-1. The product's components are lotus (NELUMBO NUCIFERA) flower water, butylene glycol, broom leaf extract of Australian tea (LEPTOSPERMUM SCOPARIUM), and extract of Stephania tetrandra (STEPHANIA TETRANDRA), in a mass ratio of 50.15:47.5:2:0.35.

[0032] In one specific embodiment, the hydroxyethylpiperazine ethanesulfonic acid was purchased from Shanghai Bilai Chemical Technology Co., Ltd., under the trade name ActiBiso-HEPES. It should be noted that the hydroxyethylpiperazine ethanesulfonic acid used in this invention can also be from other companies, and this invention does not impose any particular limitation.

[0033] In one specific embodiment, the glycyrrhizin is purchased from Jiangsu Tiansheng Pharmaceutical Co., Ltd., and its trade name is glycyrrhizin. The weight parts of the glycyrrhizin are the same as the weight parts of the product. The product consists of glycyrrhizin and cyclodextrin in a mass ratio of 10:90. It should be noted that the glycyrrhizin used in this invention can also be from other companies, and this invention does not impose any particular limitation.

[0034] In one specific embodiment, the glutathione was purchased from Dongguan Weiqi Technology Co., Ltd., under the trade name WKPep®PGSH Glutathione Plus. It should be noted that the glutathione used in this invention can also be from corresponding products of other companies; this invention does not impose any particular limitation.

[0035] In one specific embodiment, the antioxidant and whitening / brightening composition can be used in the preparation of cosmetics with corresponding effects, such as creams, emulsions, and aqueous solutions, but is not limited thereto.

[0036] In one specific embodiment, the present invention also provides an essence, preferably comprising the following components in parts by weight: 2-6 parts niacinamide, 0.5-5 parts tranexamic acid, 1-5 parts nonapeptide-1 solution, 0.5-1 part tetrahydromethylpyrimidine carboxylic acid, 0.5-2 parts pine and saffron compound extract, 0.01-0.5 parts hydroxyethylpiperazine ethanesulfonic acid, 0.01-0.5 parts glycyrrhizin, 0.0001-0.2 parts glutathione, 4-14 parts moisturizer, 0.05-0.8 parts antioxidant, 0.1-0.5 parts thickener, 0.1-0.5 parts pH adjuster, 0.01-0.5 parts chelating agent, 0.1-0.3 parts skin conditioning agent, and water to make up to 100 parts.

[0037] In one specific embodiment, the moisturizer is selected from at least one of butylene glycol, panthenol, 1,2-hexanediol, and sodium hyaluronate. For example, the moisturizer used in the following application example is butylene glycol, panthenol, 1,2-hexanediol, and sodium hyaluronate; specifically, 3 to 10 parts of butylene glycol are used, purchased from OQ Chemicals Corporation under the trade name 1,3-BG (Cosmetic Quality); 0.5 to 1 part of panthenol are used, purchased from Anhui Huaheng Biotechnology Co., Ltd. under the trade name D-panthenol; 0.5 to 3 parts of 1,2-hexanediol are used, purchased from Guangzhou Xinlongda Industrial Co., Ltd. under the trade name 1,2-hexanediol; and 0.01 to 0.1 parts of sodium hyaluronate are used, purchased from Shandong Focus Freda Biotechnology Co., Ltd. under the trade name Hyaskin® hydrolyzed sodium hyaluronate.

[0038] In one specific embodiment, the antioxidant is selected from at least one of p-hydroxyacetophenone and sodium metabisulfite. For example, in the following application examples, the antioxidants used are p-hydroxyacetophenone and sodium metabisulfite; specifically, 0.01 to 0.5 parts of p-hydroxyacetophenone are used, purchased from Symrise Fragrance & Flavor (Nantong) Co., Ltd., under the trade name SymSave® H (672096); and 0.03 to 0.1 parts of sodium metabisulfite are used, purchased from Xilong Scientific Co., Ltd., under the trade name sodium metabisulfite.

[0039] In one specific embodiment, the thickener is an acrylate / C10-30 alkanol acrylate crosspolymer, purchased from LLubrizol Advanced Materials, Inc., under the trade name CARBOPOL® Ultrez 20Polymer.

[0040] In one specific embodiment, the pH adjuster is tromethamine, purchased from Yalu Chemical Trading (Shanghai) Co., Ltd., under the trade name TRIS AMINO™ ULTRA PC, Tromethamine.

[0041] In one specific embodiment, the skin conditioning agent is allantoin, purchased from Shandong Ailitong New Materials Co., Ltd., and marketed as allantoin.

[0042] In one specific embodiment, the chelating agent is disodium EDTA, purchased from Nourion Chemicals (Ningbo) Co., Ltd., under the trade name Dissolvine Na2.

[0043] In the following examples and application examples, the whitening and moisturizing composition and serum can be prepared using conventional methods, and the present invention does not impose any particular limitations.

[0044] Example 1 This embodiment provides a serum comprising an antioxidant and whitening / brightening composition, wherein the antioxidant and whitening / brightening composition comprises niacinamide, tranexamic acid, nonapeptide-1 solution, tetrahydromethylpyrimidine carboxylic acid, pine saffron complex extract, hydroxyethylpiperazine ethanesulfonic acid, glycyrrhizin, and glutathione. The serum also includes emollients, emulsifiers, emulsion stabilizers, preservatives, thickeners, pH adjusters, skin protectants, chelating agents, and water. The serum, in parts by weight of 100, specifically comprises the components and their weight percentages shown in Table 1.

[0045] The preparation steps of the serum are as follows: 1. Nicotinamide, tranexamic acid, tetrahydromethylpyrimidine carboxylic acid, hydroxyethylpiperazine ethanesulfonic acid, glycyrrhizin, tromethamine, and sodium metabisulfite were each pre-dissolved in a small amount of water; a portion of butanediol was mixed with p-hydroxyacetophenone and 1,2-hexanediol, and the solvent was stirred until homogeneous to obtain mixture 1. 2. Add the remaining water, remaining butanediol, panthenol, acrylate / C10-30 alkanol acrylate crosspolymer, allantoin, disodium EDTA, and hydrolyzed sodium hyaluronate to a mixing bowl, heat to 80-85℃, and stir for 30 minutes until dissolved and homogeneous. 3. Cool to 65℃, add pre-dissolved tromethamine, homogenize at 1500 rpm for 1 min, and stir for 15-20 min until dissolved and homogeneous; 4. Cool to 50℃, add mixture 1, and stir until dissolved and homogeneous; 5. Cool to 38℃, add nonapeptide-1 solution, pine saffron compound extract and pre-dissolved nicotinamide, tranexamic acid, sodium metabisulfite, tetrahydromethylpyrimidine carboxylic acid, hydroxyethylpiperazine ethanesulfonic acid, glycyrrhizin and glutathione, and stir until dissolved. 6. Cool to 35℃, vacuum, filter and remove from the pot to obtain the product.

[0046] Example 2 This embodiment provides a serum comprising an antioxidant and whitening / brightening composition, wherein the antioxidant and whitening / brightening composition comprises niacinamide, tranexamic acid, nonapeptide-1 solution, tetrahydromethylpyrimidine carboxylic acid, pine saffron complex extract, hydroxyethylpiperazine ethanesulfonic acid, glycyrrhizin, and glutathione. The serum also includes emollients, emulsifiers, emulsion stabilizers, preservatives, thickeners, pH adjusters, skin protectants, chelating agents, and water. The serum, in parts by weight of 100, specifically comprises the components and their weight percentages shown in Table 1.

[0047] The preparation steps of the essence are the same as in Example 1.

[0048] Example 3 This embodiment provides a serum comprising an antioxidant and whitening / brightening composition, wherein the antioxidant and whitening / brightening composition comprises niacinamide, tranexamic acid, nonapeptide-1 solution, tetrahydromethylpyrimidine carboxylic acid, pine saffron complex extract, hydroxyethylpiperazine ethanesulfonic acid, glycyrrhizin, and glutathione. The serum also includes emollients, emulsifiers, emulsion stabilizers, preservatives, thickeners, pH adjusters, skin protectants, chelating agents, and water. The serum, in parts by weight of 100, specifically comprises the components and their weight percentages shown in Table 1.

[0049] The preparation steps of the essence are the same as in Example 1.

[0050] Example 4 This embodiment provides a serum comprising an antioxidant and whitening / brightening composition, wherein the antioxidant and whitening / brightening composition comprises niacinamide, tranexamic acid, nonapeptide-1 solution, tetrahydromethylpyrimidine carboxylic acid, pine saffron complex extract, hydroxyethylpiperazine ethanesulfonic acid, glycyrrhizin, and glutathione. The serum also includes emollients, emulsifiers, emulsion stabilizers, preservatives, thickeners, pH adjusters, skin protectants, chelating agents, and water. The serum, in parts by weight of 100, specifically comprises the components and their weight percentages shown in Table 1.

[0051] The preparation steps of the essence are the same as in Example 1.

[0052] Example 5 This embodiment provides a serum comprising an antioxidant and whitening / brightening composition, wherein the antioxidant and whitening / brightening composition comprises niacinamide, tranexamic acid, nonapeptide-1 solution, tetrahydromethylpyrimidine carboxylic acid, pine saffron complex extract, hydroxyethylpiperazine ethanesulfonic acid, glycyrrhizin, and glutathione. The serum also includes emollients, emulsifiers, emulsion stabilizers, preservatives, thickeners, pH adjusters, skin protectants, chelating agents, and water. The serum, in parts by weight of 100, specifically comprises the components and their weight percentages shown in Table 1.

[0053] The preparation steps of the essence are the same as in Example 1.

[0054] Example 6 This embodiment provides a serum comprising an antioxidant and whitening / brightening composition, wherein the antioxidant and whitening / brightening composition comprises niacinamide, tranexamic acid, nonapeptide-1 solution, tetrahydromethylpyrimidine carboxylic acid, pine saffron complex extract, hydroxyethylpiperazine ethanesulfonic acid, glycyrrhizin, and glutathione. The serum also includes emollients, emulsifiers, emulsion stabilizers, preservatives, thickeners, pH adjusters, skin protectants, chelating agents, and water. The serum, in parts by weight of 100, specifically comprises the components and their weight percentages shown in Table 1.

[0055] The preparation steps of the essence are the same as in Example 1.

[0056] Example 7 This embodiment provides a serum comprising an antioxidant and whitening / brightening composition, wherein the antioxidant and whitening / brightening composition comprises niacinamide, tranexamic acid, nonapeptide-1 solution, tetrahydromethylpyrimidine carboxylic acid, pine saffron complex extract, hydroxyethylpiperazine ethanesulfonic acid, glycyrrhizin, and glutathione. The serum also includes emollients, emulsifiers, emulsion stabilizers, preservatives, thickeners, pH adjusters, skin protectants, chelating agents, and water. The serum, in parts by weight of 100, specifically comprises the components and their weight percentages shown in Table 1.

[0057] The preparation steps of the essence are the same as in Example 1.

[0058] Table 1 Comparative Example 1 This embodiment provides a serum comprising an antioxidant and whitening / brightening composition, wherein the antioxidant and whitening / brightening composition comprises niacinamide, tranexamic acid, nonapeptide-1 solution, tetrahydromethylpyrimidine carboxylic acid, pine saffron complex extract, hydroxyethylpiperazine ethanesulfonic acid, glycyrrhizin, and glutathione. The serum also includes emollients, emulsifiers, emulsion stabilizers, preservatives, thickeners, pH adjusters, skin protectants, chelating agents, and water. The serum, in parts by weight of 100, specifically comprises the components and their weight percentages shown in Table 2.

[0059] The preparation steps of the essence are the same as in Example 1.

[0060] Comparative Example 2 This embodiment provides a serum comprising an antioxidant and whitening / brightening composition, wherein the antioxidant and whitening / brightening composition comprises niacinamide, tranexamic acid, nonapeptide-1 solution, tetrahydromethylpyrimidine carboxylic acid, pine saffron complex extract, hydroxyethylpiperazine ethanesulfonic acid, glycyrrhizin, and glutathione. The serum also includes emollients, emulsifiers, emulsion stabilizers, preservatives, thickeners, pH adjusters, skin protectants, chelating agents, and water. The serum, in parts by weight of 100, specifically comprises the components and their weight percentages shown in Table 2.

[0061] The preparation steps of the essence are the same as in Example 1.

[0062] Comparative Example 3 This embodiment provides a serum comprising an antioxidant and whitening / brightening composition, wherein the antioxidant and whitening / brightening composition comprises niacinamide, tranexamic acid, nonapeptide-1 solution, tetrahydromethylpyrimidine carboxylic acid, pine saffron complex extract, hydroxyethylpiperazine ethanesulfonic acid, glycyrrhizin, and glutathione. The serum also includes emollients, emulsifiers, emulsion stabilizers, preservatives, thickeners, pH adjusters, skin protectants, chelating agents, and water. The serum, in parts by weight of 100, specifically comprises the components and their weight percentages shown in Table 2.

[0063] The preparation steps of the essence are the same as in Example 1.

[0064] Comparative Example 4 This embodiment provides a serum comprising an antioxidant and whitening / brightening composition, wherein the antioxidant and whitening / brightening composition comprises niacinamide, tranexamic acid, carnosine (purchased from Symrise, trade name Yujiling), tetrahydromethylpyrimidine carboxylic acid, pine saffron complex extract, hydroxyethylpiperazine ethanesulfonic acid, glycyrrhizin, and glutathione. The serum also includes emollients, emulsifiers, emulsion stabilizers, preservatives, thickeners, pH adjusters, skin protectants, chelating agents, and water. The serum, in parts by weight of 100, specifically comprises the components and their weight percentages shown in Table 2.

[0065] The preparation steps of the essence are the same as in Example 1.

[0066] Comparative Example 5 This embodiment provides a serum comprising an antioxidant and whitening / brightening composition, wherein the antioxidant and whitening / brightening composition comprises niacinamide, tranexamic acid, nonapeptide-1 solution, tetrahydromethylpyrimidine carboxylic acid, pine saffron complex extract, hydroxyethylpiperazine ethanesulfonic acid, glycyrrhizin, and glutathione. The serum also includes emollients, emulsifiers, emulsion stabilizers, preservatives, thickeners, pH adjusters, skin protectants, chelating agents, and water. The serum, in parts by weight of 100, specifically comprises the components and their weight percentages shown in Table 2.

[0067] The preparation steps of the essence are the same as in Example 1.

[0068] Comparative Example 6 This embodiment provides a serum comprising an antioxidant and whitening / brightening composition, wherein the antioxidant and whitening / brightening composition comprises niacinamide, tranexamic acid, nonapeptide-1 solution, tetrahydromethylpyrimidine carboxylic acid, Atractylodes macrocephala extract (purchased from Ganzhou Baizhentang Fragrance Co., Ltd., trade name: Atractylodes macrocephala extract), hydroxyethylpiperazine ethanesulfonic acid, glycyrrhizin, and glutathione. The serum also includes emollients, emulsifiers, emulsion stabilizers, preservatives, thickeners, pH adjusters, skin protectants, chelating agents, and water. The serum, in parts by weight of 100, specifically comprises the components and their parts by weight shown in Table 2.

[0069] The preparation steps of the essence are the same as in Example 1.

[0070] Table 2 Efficacy verification: 1. Antioxidant efficacy test – ABTS free radical scavenging experiment The specific steps are as follows: 1) Dilute Trolox powder to the concentration gradient in Table 3 using PBS buffer and set aside.

[0071] Table 3 System Reference Concentration Design Table 2) The essence prepared in each example and comparative example was used as the test solution. The reaction system was prepared in a 96-well plate according to the amount of each reagent added in Table 4. The mixture was mixed and three replicates and one background control well were set for each concentration.

[0072] Table 4 ABTS Free Radical Scavenging Test Reaction System 3) After incubating at room temperature for 2-6 minutes, read the absorbance OD value at 734 nm and calculate the scavenging rate of the test solution on ABTS free radicals.

[0073] The formula for calculating the clearance rate is as follows: In the formula: C1—absorbance value of the blank ABTS system; C2 – Absorbance value of the blank system without ABTS; T1 – The absorbance value of the sample group in the ABTS system; T2 – Absorbance value of the sample group without ABTS.

[0074] The measured ABTS radical scavenging rate (average value) results are shown in Table 5.

[0075] Table 5 As shown in Table 5, the serums prepared using the antioxidant and whitening / brightening compositions of Examples 1-7 all achieved a scavenging rate of over 58% for ABTS free radicals, indicating excellent antioxidant capabilities. A comparison between Examples 1 and Examples 2-3, and between Examples 5 and Example 4, reveals that using a specific weight ratio of nonapeptide-1 solution, pine sap extract, and glycyrrhizin of 2-3:1-2:0.1-0.3 further enhances the scavenging rate of ABTS free radicals. In particular, the serum prepared using Example 1 exhibited the best scavenging rate of 65.71% for ABTS free radicals.

[0076] As can be seen from the comparison between Example 6 and Comparative Examples 1-2 and 5, and the comparison between Example 5 and Comparative Example 3, the absence of Pine and Red Plum Complex Extract, Hydroxyethylpiperazine Ethanesulfonic Acid, Glycyrrhizin, or Glutathione in the antioxidant and whitening brightening composition leads to varying degrees of reduction in the scavenging rate of ABTS free radicals in the prepared serum. Furthermore, the comparison between Example 5 and Comparative Example 4, and the comparison between Example 7 and Comparative Example 6, shows that replacing the nonapeptide-1 solution in the antioxidant and whitening brightening composition with carnosine or replacing the Pine and Red Plum Complex Extract with Atractylodes macrocephala extract also significantly reduces the scavenging rate of ABTS free radicals in the prepared serum. This demonstrates that the specific addition of nonapeptide-1, Pine and Red Plum Complex Extract, Hydroxyethylpiperazine Ethanesulfonic Acid, Glycyrrhizin, and Glutathione in this invention can produce a synergistic effect among the components, further improving the antioxidant performance.

[0077] 2. Whitening and Brightening Efficacy Test – Test of Inhibitory Ability of B16F10 Cell Tyrosinase Activity The specific steps are as follows: 1) Collect B16F10 cells in the logarithmic growth phase, at a cell density of 2×10⁶ cells / year. 4 Cells were seeded per well in a 96-well plate. After culturing in an incubator (37°C, 5% CO2) for 24 h, each test sample solution (diluted from the essence prepared in each example and comparative example) at a concentration of 1.2% (v / v) was added as the drug treatment group, kojic acid (3 mg / mL) was added as the positive control group, and untreated cells were used as the negative control group. Each group was set up in 3 replicates.

[0078] 2) Then, continue culturing in an incubator (37℃, 5% CO2) for 48 h, discard the culture medium, wash twice with PBS, add 100 μL of 0.5% Triton X-100 to each well, lyse in a -80℃ freezer for 2 h, take out the 96-well plate, let it thaw naturally at room temperature, add 50 μL of 1 mg / mL levodopa to each well, incubate at 37℃ for 2 h, read the absorbance value A of each group at 475 nm and calculate the inhibition rate of cellular tyrosinase activity.

[0079] The formula for calculating the inhibition rate of cellular tyrosinase activity is as follows: The results (average) of the inhibition rate of cellular tyrosinase activity measured in each drug administration group are shown in Table 6.

[0080] Table 6 As shown in Table 6, the serums prepared using the antioxidant and whitening / brightening compositions of Examples 1-7 all exhibited an inhibition rate of over 51% on cellular tyrosinase activity at a concentration of 1.2% (v / v), indicating a very good whitening / brightening effect. A comparison of Example 6 with Comparative Examples 1-2 and 5 shows that the absence of Pineapple and Red Plum Complex Extract, Hydroxyethylpiperazine Ethanesulfonic Acid, or Glutathione in the antioxidant and whitening / brightening compositions resulted in varying degrees of reduction in the ABTS free radical scavenging rate of the prepared serums. Further comparisons of Example 5 with Comparative Example 4 and Example 7 with Comparative Example 6 show that replacing the nonapeptide-1 solution in the antioxidant and whitening / brightening compositions with carnosine or replacing the Pineapple and Red Plum Complex Extract with Atractylodes macrocephala extract also significantly reduced the inhibition rate of cellular tyrosinase activity in the prepared serums. This demonstrates that the specific addition of nonapeptide-1, pine syrup compound extract, hydroxyethylpiperazine ethane sulfonic acid, and glutathione to this invention can create a synergistic effect among the components, further enhancing the whitening and brightening effect.

[0081] This invention has many specific applications, and the above description is only a preferred embodiment. It should be noted that the above embodiments are for illustrative purposes only and are not intended to limit the scope of protection of this invention. For those skilled in the art, several improvements can be made without departing from the principle of this invention, and these improvements should also be considered within the scope of protection of this invention.

Claims

1. An antioxidant and whitening lightening composition characterized in that, nicotinamide 2-6 parts, tranexamic acid 0.5-5 parts, nonapeptide-1 solution 1-5 parts, tetrahydro methyl pyrimidine carboxylic acid 0.5-1 part, Song Hongmei composite extract 0.5-2 parts, hydroxyethyl piperazine ethane sulfonic acid 0.01-0.5 parts, glabridin 0.01-0.5 parts, glutathione 0.0001-0.2 parts.

2. The antioxidant and whitening-boosting composition of claim 1, wherein The weight ratio of the nonapeptide-1 solution, Song Hongmei composite extract and glabridin is 2-3:1-2:0.1-0.

3.

3. Use of the antioxidant and whitening composition according to any one of claims 1-2 in the preparation of a cosmetic product.

4. Use according to claim 3, characterized in that, The dosage form of the cosmetic product is selected from any one of a cream, a lotion and an aqueous agent.

5. An essence characterized by, The antioxidant and whitening composition according to any one of claims 1-3 further comprises at least one of a humectant, an antioxidant, a thickening agent, a pH adjusting agent, a chelating agent, a skin conditioning agent and water.

6. The serum of claim 5, wherein, The essence comprises the following components in parts by weight: nicotinamide 2-6 parts, tranexamic acid 1-4 parts, nonapeptide-1 solution 1-5 parts, tetrahydro methyl pyrimidine carboxylic acid 0.5-1 part, Song Hongmei composite extract 0.5-2 parts, hydroxyethyl piperazine ethane sulfonic acid 0.01-0.5 parts, glabridin 0.01-0.5 parts, glutathione 0.0001-0.2 parts, a humectant 4-14 parts, an antioxidant 0.05-0.8 parts, a thickening agent 0.1-0.5 parts, a pH adjusting agent 0.1-0.5 parts, a chelating agent 0.01-0.5 parts, a skin conditioning agent 0.1-0.3 parts, and water to make up 100 parts.

7. The serum of claim 6, wherein the serum is characterized by, The humectant is selected from at least one of butylene glycol, panthenol, 1,2-hexanediol and sodium hyaluronate. The antioxidant is selected from at least one of p-hydroxyacetophenone and sodium metabisulfite.

8. The serum of claim 6, wherein, The thickening agent is selected from acrylates / C10-30 alkyl acrylate crosspolymer. The pH adjusting agent is selected from tromethamine. The skin conditioning agent is selected from allantoin. The chelating agent is selected from disodium EDTA.

9. The serum according to claim 7 or 8, characterized in that, The essence comprises the following components in parts by weight: nicotinamide 2-6 parts, tranexamic acid 1-4 parts, nonapeptide-1 solution 1-5 parts, tetrahydro methyl pyrimidine carboxylic acid 0.5-1 part, Song Hongmei composite extract 0.5-2 parts, hydroxyethyl piperazine ethane sulfonic acid 0.01-0.5 parts, glabridin 0.01-0.5 parts, glutathione 0.0001-0.2 parts, butylene glycol 3-10 parts, panthenol 0.5-1 part, 1,2-hexanediol 0.5-3 parts, sodium hyaluronate 0.01-0.1 part, p-hydroxyacetophenone 0.01-0.5 part, sodium metabisulfite 0.03-0.1 part, acrylates / C10-30 alkyl acrylate crosspolymer 0.1-0.5 part, tromethamine 0.1-0.5 part, disodium EDTA 0.01-0.5 part, allantoin 0.1-0.3 part, and water to make up 100 parts.

10. A method of preparing the serum according to any one of claims 5-9, characterized in that, The method comprises the following steps: After the components are weighed according to the weight ratio, they are mixed and stirred uniformly to obtain the essence. The method comprises the following steps:

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