Epimedium seed stratification treatment method
By evaluating the respiration rate and α-amylase activity of Epimedium seeds, and by graded treatment combined with gibberellin 4 and temperature-dependent stratification, the problem of unstable treatment effects of Epimedium seeds was solved, and the germination rate and seedling growth quality were improved.
Patent Information
- Application Number
- CN202511449453.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-10-11
- Publication Date
- 2025-11-14
AI Technical Summary
The physiological state of Epimedium seeds varies naturally, resulting in different seeds responding differently to treatment. Existing technologies make it difficult to achieve stable and consistent effects from seed deposition treatment.
Seed physiological status was assessed by respiration intensity and α-amylase activity, and the seeds were classified into three categories: high vigor, medium vigor, and low vigor. The seeds were treated with gibberellin 4 vacuum permeation treatment and temperature-controlled stratification, with the temperature and time adjusted. A mixed substrate of river sand and perlite was used for seed treatment.
It improves seed germination rate and seedling growth quality, ensures the stability and consistency of seed treatment for each batch, and promotes the physiological and morphological after-ripening of seeds.
Abstract
Description
Technical Field
[0001] This invention relates to the field of Chinese medicinal herb cultivation technology, specifically to a method for stratification treatment of Epimedium seeds. Background Technology
[0002] The physiological state of Epimedium seeds exhibits natural variation: even seeds from the same batch may show certain natural variations in their physiological state. These variations may include seed maturity, vigor, enzyme activity, hormone levels, etc. Seeds in different physiological states respond differently to treatments: seeds in different physiological states may respond differently to temperature-dependent stratification treatments. For example, seeds with high vigor may require a shorter stratification time, while seeds with low vigor may require a longer stratification time.
[0003] Epimedium brevicornu Maxim. is a perennial evergreen C3 plant belonging to the genus Epimedium in the family Berberidaceae. It exhibits severe physiological and morphological seed dormancy. Morphological dormancy occurs when the embryo is not fully developed and requires approximately 25°C to continue differentiating and elongating. Physiological dormancy (PD) is characterized by high ABA levels in the seed coat / endosperm. Summary of the Invention
[0004] As described in the prior art above, the purpose of this invention is to provide a method for treating Epimedium seeds by stratification. This method can not only improve the effect of seed deposition treatment, but also improve the stability and consistency of the deposition treatment effect of each batch of seeds according to their physiological state.
[0005] The objective of this invention is achieved through the following technical solution: The method for stratification treatment of Epimedium seeds includes the following steps: S1. Initial Physiological Status Assessment of Seeds: Seeds of the same batch of *Epimedium wushanense* T.S. Ying were collected. A portion of the seed samples were randomly selected for respiration intensity measurement. These seeds were placed in a sealed respiration chamber, and changes in gas composition within the chamber were continuously monitored using a high-precision gas analyzer (such as IRGA or MS). The amount of CO2 released and O2 consumed per unit time was calculated. After the respiration intensity measurement, hyperspectral imaging technology was used to acquire spectral reflectance images of these seed samples. Spectral reflectance images of the seeds at different wavelengths were obtained, and the data were recorded. Image analysis software was used to process the spectral reflectance images at different wavelengths to extract the α-amylase absorption wavelength. Spectral reflectance; then, this portion of seed samples was subjected to destructive testing to determine the enzyme activity of the key enzyme α-amylase in this portion of seed samples, which served as a batch of seed samples with known α-amylase enzyme activity; through statistical analysis, a relationship model between spectral reflectance at the absorption wavelength of α-amylase and α-amylase enzyme activity was established; using the established relationship model, the enzyme activity of α-amylase in other portions of seed samples was evaluated; the specific method for determining the enzyme activity of the key enzyme α-amylase in seeds is as follows: the seed coat was removed, the seeds were ground using pH 7.0, 0.1M phosphate buffer, centrifuged, and the supernatant was used as the enzyme extraction solution from the embryo or endosperm, and the enzyme activity of the key enzyme α-amylase was determined using an ELISA reader or a fluorescence spectrometer. S2. Seed screening and classification: Based on respiration intensity and spectral reflectance at the absorption wavelength of α-amylase, seeds are divided into three categories: high vigor, medium vigor, and low vigor. High vigor seeds are directly subjected to rapid germination treatment, medium vigor seeds are subjected to appropriate after-ripening treatment, and low vigor seeds are subjected to longer after-ripening treatment or are eliminated. S3. Warm stratification treatment: River sand is sterilized at 121℃ for 1 hour and then naturally cooled to room temperature. The river sand is mixed with perlite and vermiculite. The seeds and river sand are mixed evenly in a ratio of 1:3, and the moisture content is kept at 60%. According to the seed classification, the deposition temperature, deposition time and the concentration of gibberellin 4 for vacuum infiltration treatment are adjusted accordingly. The mixed seeds are then subjected to warm stratification treatment.
[0006] S4. Cold stratification treatment: Place the seeds that have undergone warm stratification treatment in an environment of 4℃ for 20 days to perform cold stratification treatment.
[0007] Furthermore, the term "uniform batch" refers to seeds that should be collected from the same planting area and harvested within the same time period during a specific harvest season.
[0008] Furthermore, the conditions for the warm stratification treatment of the high-vitality seeds are as follows: warm deposition temperature of 25°C for 15-20 days, and hormone treatment using a low concentration of 20-30 mg·L⁻¹. -1 Gibberellin 4 was subjected to vacuum permeation treatment for 20-30 minutes.
[0009] Furthermore, the conditions for the warm stratification treatment of the medium-vitality seeds are as follows: warm deposition temperature of 25°C for 25-30 days, and hormone treatment using a moderate concentration of 30-50 mg·L⁻¹. -1 Gibberellin 4 was subjected to vacuum permeation treatment for 30-40 minutes.
[0010] Furthermore, the conditions for the warm stratification treatment of the low-vitality seeds are as follows: warm deposition temperature of 25°C for 25-30 days, and hormone treatment using a high concentration of 30-50 mg·L⁻¹. -1 Gibberellin 4 was subjected to vacuum permeation treatment for 30-40 minutes.
[0011] Furthermore, fatty acid degradation treatment is carried out before the warm stratification treatment in step S3: the seeds are soaked in a lipase solution with a concentration of 0.1-1 mg / mL for 6-12 hours. After soaking, the seeds are taken out and rinsed with clean water to remove residual lipase.
[0012] Furthermore, the seeds that have undergone fatty acid degradation treatment are soaked in a 0.1-0.5M thiourea solution for 12-24 hours.
[0013] Furthermore, the cold-stratification treated seeds are transferred to a 15°C light environment for light germination treatment; the light-germinated seeds are then removed, rinsed with clean water, and then sown normally.
[0014] Further, in step S1, after collecting the seeds, they are soaked in a 0.1% potassium permanganate solution for 15-30 minutes for disinfection.
[0015] Compared with the prior art, the beneficial effects of the present invention are as follows: The Epimedium seed stratification treatment method provided by this invention, combining two methods, can more comprehensively promote the physiological and morphological after-ripening of seeds, improving seed germination rate and seedling growth quality. Combining respiration and enzyme activity measurements allows for a more comprehensive assessment of the initial physiological state of the seeds. Respiration measurements provide information on the overall metabolic activity of the seeds, while enzyme activity measurements provide information on specific metabolic processes within the seeds. Furthermore, establishing a model relating spectral reflectance at the absorption wavelength of α-amylase to its enzyme activity allows for non-destructive measurement of α-amylase activity using hyperspectral imaging technology, facilitating practical application. Epimedium seeds exhibit a "small embryo + physiological dormancy" type (MPD), with gibberellin 4 activity 3-10 times higher than GA3. It can directly bind to the GID1 receptor, rapidly triggering α-amylase and cell wall relaxation protein genes, promoting embryo development and breaking seed dormancy. Seed screening and classification allow for more precise selection of temperature-dependent stratification conditions suitable for each batch of seeds. For example, seeds with active respiration and high enzyme activity require shorter stratification times, while seeds with weak respiration and low enzyme activity may require longer stratification times. Fatty acid degradation treatment can further enhance the metabolic activity of seeds, making them more responsive to temperature changes during temperature-dependent stratification, thereby increasing germination rates. River sand mixed with perlite and vermiculite, with seeds and sand mixed evenly in a 1:3 ratio and maintaining a moisture content of 60%, provides an ideal environment for seed embryo maturation due to the permeability and water retention of this matrix. Using the method of this invention not only improves the effectiveness of seed deposition treatment but also enhances the stability and consistency of the deposition treatment effect for each batch of seeds based on their physiological state. Detailed Implementation
[0016] The present invention will now be further described in conjunction with specific embodiments. It should be noted that, without conflict, the embodiments or technical features described below can be arbitrarily combined to form new embodiments.
[0017] Example 1
[0018] This embodiment provides a method for stratification treatment of Epimedium seeds, including the following steps: S1. Initial Physiological Status Assessment of Seeds: Seeds of the same batch of *Epimedium wushanense* T.S. Ying were collected. A portion of the seed samples were randomly selected for respiration intensity measurement. These seeds were placed in a sealed respiration chamber, and changes in gas composition within the chamber were continuously monitored using a high-precision gas analyzer (such as IRGA or MS). The amount of CO2 released and O2 consumed per unit time was calculated. After the respiration intensity measurement, hyperspectral imaging technology was used to acquire spectral reflectance images of these seed samples. Spectral reflectance images of the seeds at different wavelengths were obtained, and the data were recorded. Image analysis software was used to process the spectral reflectance images at different wavelengths to extract the α-amylase absorption wavelength. Spectral reflectance; then, this portion of seed samples was subjected to destructive testing to determine the enzyme activity of the key enzyme α-amylase in this portion of seed samples, which served as a batch of seed samples with known α-amylase enzyme activity; through statistical analysis, a relationship model between spectral reflectance at the absorption wavelength of α-amylase and α-amylase enzyme activity was established; using the established relationship model, the enzyme activity of α-amylase in other portions of seed samples was evaluated; the specific method for determining the enzyme activity of the key enzyme α-amylase in seeds is as follows: the seed coat was removed, the seeds were ground using pH 7.0, 0.1M phosphate buffer, centrifuged, and the supernatant was used as the enzyme extraction solution from the embryo or endosperm, and the enzyme activity of the key enzyme α-amylase was determined using an ELISA reader or a fluorescence spectrometer. S2. Seed screening and classification: Based on respiration intensity and spectral reflectance at the absorption wavelength of α-amylase, seeds are divided into three categories: high vigor, medium vigor, and low vigor. High vigor seeds are directly subjected to rapid germination treatment, medium vigor seeds are subjected to appropriate after-ripening treatment, and low vigor seeds are subjected to longer after-ripening treatment or are eliminated. S3. Warm stratification treatment: River sand is sterilized at 121℃ for 1 hour and then naturally cooled to room temperature. The river sand is mixed with perlite and vermiculite. The seeds and river sand are mixed evenly in a ratio of 1:3, and the moisture content is kept at 60%. According to the seed classification, the deposition temperature, deposition time and the concentration of gibberellin 4 for vacuum infiltration treatment are adjusted accordingly. The mixed seeds are then subjected to warm stratification treatment.
[0019] S4. Cold stratification treatment: Place the seeds that have undergone warm stratification treatment in an environment of 4℃ for 20 days to perform cold stratification treatment.
[0020] In this embodiment, "uniform batch" refers to seeds that should be collected from the same planting area and harvested within the same time period during a specific harvest season.
[0021] In this embodiment, the conditions for warm stratification treatment of high-vitality seeds are: warm deposition temperature of 25°C, duration of 15-20 days, and hormone treatment using a low concentration of 20-30 mg·L⁻¹. -1 Gibberellin 4 was subjected to vacuum permeation treatment for 20-30 minutes.
[0022] In this embodiment, the conditions for warm stratification treatment of medium-vitality seeds are as follows: warm deposition temperature of 25°C, duration of 25-30 days, and hormone treatment using a moderate concentration of 30-50 mg·L⁻¹. -1 Gibberellin 4 was subjected to vacuum permeation treatment for 30-40 minutes.
[0023] In this embodiment, the conditions for warm stratification treatment of low-vitality seeds are as follows: warm deposition temperature of 25°C, duration of 25-30 days, and hormone treatment using a high concentration of 30-50 mg·L⁻¹. -1 Gibberellin 4 was subjected to vacuum permeation treatment for 30-40 minutes.
[0024] Significance of measuring α-amylase activity: Because starch breakdown directly affects seed germination rate and early growth, α-amylase activity can directly reflect the energy metabolism status of seeds and is used to assess seed germination ability.
[0025] Mechanism of action of gibberellin-4 Gibberellin 4 (GA4) is a highly active gibberellin, with an activity 3-10 times higher than GA3 (gibberellin 3). Gibberellin 4 can directly bind to the GID1 receptor, thereby triggering a series of physiological responses and promoting seed germination. The following is the specific mechanism of action of gibberellin 4 in the seeds of Epimedium wushanense: 1. Direct binding to the GID1 receptor: Gibberellin 4 can directly bind to the GID1 receptor, which is a key step in gibberellin signaling. After the GID1 receptor binds to gibberellin 4, it triggers a series of downstream signal transductions, thereby breaking the seed's dormancy state.
[0026] 2. Promoting the synthesis of α-amylase: α-amylase is a key enzyme that breaks down starch, providing energy for seed germination. Gibberellin 4 can rapidly trigger the expression of the α-amylase gene, increasing α-amylase synthesis, thereby promoting the breakdown of starch within the seed and providing the necessary energy for seed germination.
[0027] 3. Promoting the expression of cell wall relaxin genes. Expansins are proteins that relax cell walls, facilitating cell elongation and expansion. Gibberellin 4 can rapidly trigger the expression of cell wall relaxin genes, increasing the synthesis of cell wall relaxins, thereby promoting embryo development and seed germination.
[0028] 4. Relieving the Inhibitory Effect of ABA: ABA is a hormone that inhibits seed germination; high concentrations of ABA will suppress seed germination. Gibberellin 4 can relieve the inhibitory effect of ABA through multiple mechanisms, including: reducing ABA synthesis: Gibberellin 4 can inhibit the activity of ABA synthase, reducing ABA synthesis. Promoting ABA degradation: Gibberellin 4 can activate the activity of ABA degrading enzymes, accelerating ABA degradation. Competitive binding: Gibberellin 4 and ABA compete for binding in signal transduction pathways; Gibberellin 4 can competitively bind to relevant receptors, thereby relieving the inhibitory effect of ABA.
[0029] 5. Promoting Embryo Development: Since the embryos of Epimedium wushanense seeds are relatively small, they require further development to reach a germination stage. Gibberellin-4 can promote embryo development through the following mechanisms: Promoting Cell Division and Elongation: Gibberellin-4 can activate cytokinin and auxin signaling, promoting embryonic cell division and elongation. Providing Necessary Energy: By promoting the synthesis of α-amylase, gibberellin-4 can provide the necessary energy for embryo development.
[0030] Example 2
[0031] This embodiment provides a method for stratification treatment of Epimedium seeds, including the following steps: S1. Initial Physiological Status Assessment of Seeds: Seeds of the same batch of *Epimedium wushanense* T.S. Ying were collected. A portion of the seed samples were randomly selected for respiration intensity measurement. These seeds were placed in a sealed respiration chamber, and changes in gas composition within the chamber were continuously monitored using a high-precision gas analyzer (such as IRGA or MS). The amount of CO2 released and O2 consumed per unit time was calculated. After the respiration intensity measurement, hyperspectral imaging technology was used to acquire spectral reflectance images of these seed samples. Spectral reflectance images of the seeds at different wavelengths were obtained, and the data were recorded. Image analysis software was used to process the spectral reflectance images at different wavelengths to extract the α-amylase absorption wavelength. Spectral reflectance; then, this portion of seed samples was subjected to destructive testing to determine the enzyme activity of the key enzyme α-amylase in this portion of seed samples, which served as a batch of seed samples with known α-amylase enzyme activity; through statistical analysis, a relationship model between spectral reflectance at the absorption wavelength of α-amylase and α-amylase enzyme activity was established; using the established relationship model, the enzyme activity of α-amylase in other portions of seed samples was evaluated; the specific method for determining the enzyme activity of the key enzyme α-amylase in seeds is as follows: the seed coat was removed, the seeds were ground using pH 7.0, 0.1M phosphate buffer, centrifuged, and the supernatant was used as the enzyme extraction solution from the embryo or endosperm, and the enzyme activity of the key enzyme α-amylase was determined using an ELISA reader or a fluorescence spectrometer. S2. Seed screening and classification: Based on respiration intensity and spectral reflectance at the absorption wavelength of α-amylase, seeds are divided into three categories: high vigor, medium vigor, and low vigor. High vigor seeds are directly subjected to rapid germination treatment, medium vigor seeds are subjected to appropriate after-ripening treatment, and low vigor seeds are subjected to longer after-ripening treatment or are eliminated. S3. Perform fatty acid degradation treatment: Soak the seeds in a lipase solution with a concentration of 0.1-1 mg / mL for 6-12 hours. After soaking, remove the seeds and rinse them with clean water to remove any residual lipase. S4. Warm stratification treatment: River sand is sterilized at 121℃ for 1 hour and then naturally cooled to room temperature. The river sand is mixed with perlite and vermiculite. The seeds and river sand are mixed evenly in a ratio of 1:3, and the moisture content is kept at 60%. According to the seed classification, the deposition temperature, deposition time and the concentration of gibberellin 4 for vacuum infiltration treatment are adjusted accordingly. The mixed seeds are then subjected to warm stratification treatment.
[0032] S5. Cold stratification treatment: Place the seeds that have undergone warm stratification treatment in an environment of 4℃ for 20 days to perform cold stratification treatment.
[0033] Example 3
[0034] This embodiment provides a method for stratification treatment of Epimedium seeds, which includes the following steps: S1. Initial Physiological Status Assessment of Seeds: Seeds of the same batch of *Epimedium wushanense* T.S. Ying were collected. A portion of the seed samples were randomly selected for respiration intensity measurement. These seeds were placed in a sealed respiration chamber, and changes in gas composition within the chamber were continuously monitored using a high-precision gas analyzer (such as IRGA or MS). The amount of CO2 released and O2 consumed per unit time was calculated. After the respiration intensity measurement, hyperspectral imaging technology was used to acquire spectral reflectance images of these seed samples. Spectral reflectance images of the seeds at different wavelengths were obtained, and the data were recorded. Image analysis software was used to process the spectral reflectance images at different wavelengths to extract the α-amylase absorption wavelength. Spectral reflectance; then, this portion of seed samples was subjected to destructive testing to determine the enzyme activity of the key enzyme α-amylase in this portion of seed samples, which served as a batch of seed samples with known α-amylase enzyme activity; through statistical analysis, a relationship model between spectral reflectance at the absorption wavelength of α-amylase and α-amylase enzyme activity was established; using the established relationship model, the enzyme activity of α-amylase in other portions of seed samples was evaluated; the specific method for determining the enzyme activity of the key enzyme α-amylase in seeds is as follows: the seed coat was removed, the seeds were ground using pH 7.0, 0.1M phosphate buffer, centrifuged, and the supernatant was used as the enzyme extraction solution from the embryo or endosperm, and the enzyme activity of the key enzyme α-amylase was determined using an ELISA reader or a fluorescence spectrometer. S2. Seed screening and classification: Based on respiration intensity and spectral reflectance at the absorption wavelength of α-amylase, seeds are divided into three categories: high vigor, medium vigor, and low vigor. High vigor seeds are directly subjected to rapid germination treatment, medium vigor seeds are subjected to appropriate after-ripening treatment, and low vigor seeds are subjected to longer after-ripening treatment or are eliminated. S3. Perform fatty acid degradation treatment: Soak the seeds in a lipase solution with a concentration of 0.1-1 mg / mL for 6-12 hours. After soaking, remove the seeds and rinse them with clean water to remove any residual lipase. S4. Soak the seeds that have undergone fatty acid degradation treatment in a 0.1-0.5M thiourea solution for 12-24 hours. S5. Warm stratification treatment: River sand is sterilized at 121℃ for 1 hour and then naturally cooled to room temperature. The river sand is mixed with perlite and vermiculite. The seeds and river sand are mixed evenly in a ratio of 1:3, and the moisture content is kept at 60%. According to the seed classification, the deposition temperature, deposition time and the concentration of gibberellin 4 for vacuum infiltration treatment are adjusted accordingly. The mixed seeds are then subjected to warm stratification treatment.
[0035] S6. Cold stratification treatment: Place the seeds that have undergone warm stratification treatment in an environment of 4℃ for 20 days to perform cold stratification treatment.
[0036] The role of step S4, thiourea treatment, in this embodiment: Thiourea treatment can improve the physiological state of seeds and reduce harmful substances such as free radicals and peroxides that accumulate during seed dormancy. Simultaneously, it may provide better energy and nutritional support for seed germination by regulating the metabolism of nutrients within the seed.
[0037] Thiourea treatment may enhance seed tolerance to environmental stresses such as drought, high temperature, and low temperature by modulating the seed's internal antioxidant system. It may also reduce seed damage caused by environmental stress by activating the activity of antioxidant enzymes such as superoxide dismutase and catalase.
[0038] Principle: While thiourea has a positive effect on seeds at certain concentrations, excessively high concentrations may be toxic. For example, high concentrations of thiourea may interfere with normal physiological processes in seeds, leading to cell damage and metabolic disorders. If the thiourea concentration is too high (above 0.5M), it may cause a decrease in seed germination rate, poor seedling growth, or even seed death.
[0039] The above embodiments are merely preferred embodiments of the present invention and should not be construed as limiting the scope of protection of the present invention. Any non-substantial changes and substitutions made by those skilled in the art based on the present invention shall fall within the scope of protection claimed by the present invention.
Claims
1. A method for stratification treatment of Epimedium seeds, characterized in that, The following steps are included: S1. Initial Physiological Status Assessment of Seeds: Seeds of the same batch of Epimedium wushanense were collected, and a portion of the seed samples were randomly selected for respiration intensity measurement. These seeds were placed in a sealed respiration chamber, and a high-precision gas analyzer was used to continuously monitor changes in gas composition within the chamber, calculating the CO2 release and O2 consumption per unit time. After the respiration intensity measurement, hyperspectral imaging technology was used to acquire spectral reflectance images of these seed samples at different wavelengths. Data were recorded, and image analysis software was used to process the spectral reflectance images at different wavelengths to extract the spectral reflectance at the α-amylase absorption wavelength. These seed samples were then further processed... A destructive assay was performed to determine the enzyme activity of the key enzyme α-amylase in this portion of seed samples, which served as a batch of seed samples with known α-amylase activity. A statistical analysis was conducted to establish a model relating spectral reflectance at the absorption wavelength of α-amylase to its enzyme activity. This model was then used to evaluate the α-amylase activity of other seed samples. The specific method for determining the enzyme activity of the key enzyme α-amylase in seeds was as follows: the seed coat was removed, the seeds were ground using a pH 7.0, 0.1M phosphate buffer, centrifuged, and the supernatant was used as the enzyme extraction solution for the embryo or endosperm. The enzyme activity of the key enzyme α-amylase was determined using a microplate reader or fluorescence spectrometer. S2. Seed screening and classification: Based on respiration intensity and spectral reflectance at the absorption wavelength of α-amylase, seeds are divided into three categories: high vigor, medium vigor, and low vigor. High vigor seeds are directly subjected to rapid germination treatment, medium vigor seeds are subjected to appropriate after-ripening treatment, and low vigor seeds are subjected to longer after-ripening treatment or are eliminated. S3. Warm stratification treatment: River sand is sterilized at 121℃ for 1 hour and then naturally cooled to room temperature. The river sand is mixed with perlite and vermiculite. The seeds and river sand are mixed evenly in a ratio of 1:3, and the moisture content is kept at 60%. According to the seed classification, the deposition temperature, deposition time and the concentration of gibberellin 4 for vacuum infiltration treatment are adjusted accordingly. The mixed seeds are then subjected to warm stratification treatment. S4. Cold stratification treatment: Place the seeds that have undergone warm stratification treatment in an environment of 4℃ for 20 days to perform cold stratification treatment.
2. The method for stratification treatment of Epimedium seeds as described in claim 1, characterized in that, The term "uniform batch" refers to seeds that should be collected from the same planting area and harvested within the same time period during a specific harvest season.
3. The method for stratification treatment of Epimedium seeds as described in claim 1, characterized in that, The conditions for the warm stratification treatment of the high-vitality seeds were as follows: warm deposition temperature of 25°C for 15-20 days, and hormone treatment using a low concentration of 20-30 mg / L. -1 Gibberellin 4 was subjected to vacuum permeation treatment for 20-30 minutes.
4. The method for stratification treatment of Epimedium seeds as described in claim 1, characterized in that, The warm stratification treatment conditions for the medium-vitality seeds were as follows: warm deposition temperature of 25°C, lasting for 25-30 days, and hormone treatment using a moderate concentration of 30-50 mg·L⁻¹. -1 Gibberellin 4 was subjected to vacuum permeation treatment for 30-40 minutes.
5. The method for stratification treatment of Epimedium seeds as described in claim 1, characterized in that, The conditions for the warm stratification treatment of the low-vitality seeds were: a warm deposition temperature of 25°C for 25-30 days, and hormone treatment using a high concentration of 30-50 mg·L⁻¹. -1 Gibberellin 4 was subjected to vacuum permeation treatment for 30-40 minutes.
6. The method for stratification treatment of Epimedium seeds as described in claim 1, characterized in that, Before the warm stratification treatment in step S3, fatty acid degradation treatment is carried out: the seeds are soaked in a lipase solution with a concentration of 0.1-1 mg / mL for 6-12 hours. After soaking, the seeds are taken out and rinsed with clean water to remove residual lipase.
7. The method for stratification treatment of Epimedium seeds as described in claim 6, characterized in that, Seeds that have undergone fatty acid degradation treatment are soaked in a 0.1-0.5M thiourea solution for 12-24 hours.
8. The method for stratification treatment of Epimedium seeds as described in claim 1, characterized in that, After cold stratification, the seeds were transferred to a light environment at 15°C for light germination treatment. The seeds after light germination treatment were then removed, rinsed with clean water, and then sown as usual.
9. The method for stratification treatment of Epimedium seeds as described in claim 1, characterized in that, In step S1, after collecting the seeds, they are soaked in a 0.1% potassium permanganate solution for 15-30 minutes for disinfection.