A composition for treating hyperlipidemia and hyperglycemia and a preparation method thereof

Abstract

The application belongs to the technical field of medicine, and discloses a composition for treating hyperlipidemia and hyperglycemia and a preparation method thereof, which is composed of the following raw materials in parts by weight: 10-28 parts of Herba Portulacae, 10-25 parts of Chaenomelis Fructus, 5-12 parts of Rhizoma Polygonati, 5-10 parts of Valeriana jatamansi Jones, 0.5-3 parts of Illicium verum, 0.5-4 parts of Panax notoginseng, 1-5 parts of Crataegus pinnatifida and 2-8 parts of Pu'er tea. The preparation method comprises the following steps: S1, weighing and crushing the raw materials; S2, twice water extraction of the crushed Herba Portulacae, Chaenomelis Fructus, Illicium verum, Crataegus pinnatifida and Pu'er tea to obtain an extract; S3, ethanol extraction and then ethyl acetate extraction of the crushed Valeriana jatamansi Jones and Rhizoma Polygonati to obtain an extract; and S4, mixing the crushed Panax notoginseng, the extract obtained in S2 and the extract obtained in S3 to obtain the composition for treating hyperlipidemia and hyperglycemia. The composition has a significant effect of reducing blood lipid and blood sugar.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of medicine, and particularly relates to a composition for treating hyperlipidemia and hyperglycemia and a preparation method thereof. BACKGROUND

[0002] Hyperlipidemia, also known as hyperlipemia or dyslipidemia, generally refers to an increase in plasma triglycerides (TG) and / or total cholesterol (TC), an increase in low-density lipoprotein cholesterol (LDL-C), and a decrease in high-density lipoprotein cholesterol (HDL-C). Hyperlipidemia is closely related to a variety of diseases such as atherosclerosis, coronary heart disease, stroke, etc. In recent years, the blood lipid level of the population in China has increased significantly, and the prevalence of dyslipidemia has increased year by year. At present, statins are commonly used in clinical practice to lower cholesterol, but the efficacy of statins is limited, and there is a need to optimize the strategy for lowering blood lipids and explore more comprehensive drugs for lowering blood lipids.

[0003] Hyperglycemia refers to an abnormal increase in the concentration of glucose in the blood, usually caused by insufficient insulin secretion, insulin resistance, or other metabolic abnormalities. Long-term hyperglycemia can lead to diabetes and its complications, which can cause damage to the corresponding organs, such as diabetic nephropathy, diabetic retinopathy, diabetic neuropathy, diabetic foot, etc.

[0004] At present, most of the drugs for treating hyperlipidemia and hyperglycemia in clinical practice are western medicines, and their effects are mostly single. SUMMARY

[0005] The technical problem to be solved by the present application is to provide a composition for treating hyperlipidemia and hyperglycemia to improve the effects of lowering blood lipids and blood sugar.

[0006] To solve the above technical problems, the technical solution of the present application is as follows:

[0007] A composition for treating hyperlipidemia and hyperglycemia, consisting of the following raw materials in parts by weight: 10-28 parts of Portulaca oleracea, 10-25 parts of Chaenomeles speciosa, 5-12 parts of Polygonatum sibiricum, 5-10 parts of Valeriana jatamansi, 0.5-3 parts of Illicium verum, 0.5-4 parts of Panax notoginseng, 1-5 parts of Crataegus pinnatifida, and 2-8 parts of Pu'er tea.

[0008] In the composition for treating hyperlipidemia and hyperglycemia provided by the present application, preferably, it consists of the following raw materials in parts by weight: 15-22 parts of Portulaca oleracea, 13-20 parts of Chaenomeles speciosa, 5-8 parts of Polygonatum sibiricum, 5-8 parts of Valeriana jatamansi, 1-2 parts of Illicium verum, 1-3 parts of Panax notoginseng, 3-5 parts of Crataegus pinnatifida, and 2-5 parts of Pu'er tea.

[0009] In the composition for treating hyperlipidemia and hyperglycemia provided by the present application, preferably, the following raw materials are used by weight: 20 parts of Portulaca oleracea, 16 parts of Chaenomeles speciosa, 7 parts of Polygonatum sibiricum, 7 parts of Daphne giraldii, 1 part of Illicium verum, 2 parts of Panax notoginseng, 4 parts of Crataegus pinnatifida, and 2 parts of Pu'er tea.

[0010] In the composition for treating hyperlipidemia and hyperglycemia provided by the present application, more preferably, the following raw materials are used by weight: 10-28 parts of Portulaca oleracea, 10-25 parts of Chaenomeles speciosa, 5-12 parts of Polygonatum sibiricum, 5-10 parts of Daphne giraldii, 0.5-3 parts of Illicium verum, 0.5-4 parts of Panax notoginseng, 1-5 parts of Crataegus pinnatifida, 2-8 parts of Pu'er tea, and 3-8 parts of Syzygium aromaticum.

[0011] The present application further provides a preparation method of the composition for treating hyperlipidemia and hyperglycemia, comprising the following steps:

[0012] S1, the following raw materials are weighed by weight: 10-28 parts of Portulaca oleracea, 10-25 parts of Chaenomeles speciosa, 5-12 parts of Polygonatum sibiricum, 5-10 parts of Daphne giraldii, 0.5-3 parts of Illicium verum, 0.5-4 parts of Panax notoginseng, 1-5 parts of Crataegus pinnatifida, and 2-8 parts of Pu'er tea;

[0013] S2, the crushed Portulaca oleracea, Chaenomeles speciosa, Illicium verum, Crataegus pinnatifida, and Pu'er tea are put into a container, water is added, decoction is performed, and the first medicinal liquid and the first medicinal residue are obtained by filtration; the first medicinal residue is put into the container again, water is added, decoction is performed, and the second medicinal liquid and the second medicinal residue are obtained by filtration; the first medicinal liquid and the second medicinal liquid are combined, vacuum distillation is performed, and the first extract is obtained by concentrating and drying;

[0014] S3, the crushed Daphne giraldii and Polygonatum sibiricum are put into a reflux extraction device, 75-90% Vol of ethanol is added, and reflux extraction is performed for 2 hours, and the third medicinal residue and the third medicinal liquid are obtained by filtration; the third medicinal liquid is vacuum distilled, concentrated and dried, and the second extract is obtained; the third medicinal residue is put into the reflux extraction device, ethyl acetate is added, and reflux extraction is performed for 1 hour, and the fourth medicinal liquid and the fourth medicinal residue are obtained by filtration; the fourth medicinal liquid is vacuum concentrated and dried, and the third extract is obtained by crushing;

[0015] S4, the crushed Panax notoginseng, the first extract, the second extract, and the third extract are combined and uniformly mixed, and the composition for treating hyperlipidemia and hyperglycemia is obtained.

[0016] In the preparation method of the composition for treating hyperlipidemia and hyperglycemia provided by the present application, preferably, 3-8 parts of Syzygium aromaticum are weighed by weight in step S1 and crushed.

[0017] In the preparation method of the composition for treating hyperlipidemia and hyperglycemia provided by the present application, more preferably, the crushed Syzygium aromaticum is treated in the same way as the raw materials in step S2.

[0018] In the preparation method of the composition for treating hyperlipidemia and hyperglycemia provided by the present invention, more preferably, the ethanol concentration in step S3 is 85% Vol.

[0019] In the preparation method of the composition for treating hyperlipidemia and hyperglycemia provided by the present invention, it is further preferred that the temperature of adding ethanol and heating and refluxing for extraction in step S3 is 65-72°C.

[0020] In the preparation method of the composition for treating hyperlipidemia and hyperglycemia provided by the present invention, more preferably, the heating reflux extraction temperature for adding ethyl acetate in step S3 is 68-75°C.

[0021] Purslane is the dried aerial part of *Portulaca oleracea* L., a plant in the Portulacaceae family. It is harvested in summer and autumn, after which residual roots and impurities are removed, the plant is washed, slightly steamed or scalded, and then sun-dried. It is sour and cold in nature. It enters the liver and large intestine meridians. It clears heat and detoxifies, cools the blood and stops bleeding, and stops dysentery. It is used for dysentery due to heat toxins, carbuncles and boils, eczema, erysipelas, snake and insect bites, hematochezia, hemorrhoidal bleeding, and metrorrhagia.

[0022] Papaya is the dried, nearly mature fruit of *Chaenomeles speciosa* (Sweet) Nakai, a plant in the Rosaceae family. It is harvested in summer and autumn when the fruit is greenish-yellow, scalded in boiling water until the outer skin turns grayish-white, then longitudinally split in half and sun-dried. It is sour and warm in nature. It enters the liver and spleen meridians. It relaxes muscles and tendons, harmonizes the stomach, and resolves dampness. It is used for damp-bi syndrome with contractures, lower back and knee pain, summer-heat vomiting and diarrhea, muscle spasms and pain, and beriberi with edema.

[0023] Polygonatum is the dried rhizome of *Polygonatum kingianum* Coll. et Hemsl., *Polygonatum sibiricum* Red., or *Polygonatum cyrtonema* Hua, all belonging to the Liliaceae family. Depending on its shape, it is commonly known as "Large Polygonatum," "Chicken Head Polygonatum," or "Ginger-shaped Polygonatum." It is harvested in spring and autumn, the fibrous roots are removed, it is washed, briefly blanched in boiling water or steamed until thoroughly cooked, and then dried. It is sweet and neutral in nature. It enters the spleen, lung, and kidney meridians. It tonifies qi and nourishes yin, strengthens the spleen, moistens the lungs, and benefits the kidneys. It is used for spleen and stomach qi deficiency, fatigue, stomach yin deficiency, dry mouth and poor appetite, lung deficiency and dry cough, consumptive cough with hemoptysis, deficiency of essence and blood, soreness and weakness of the lower back and knees, premature graying of hair, and internal heat and thirst.

[0024] Spider-scented herbs are the dried rhizomes and roots of the plant *Vaieriana jatamansi* Jones. Harvested in autumn, the roots and soil are removed, and then dried. They are slightly bitter, pungent, and warm in nature. They enter the heart, spleen, and stomach meridians. They regulate qi and relieve pain, promote digestion and stop diarrhea, dispel wind and dampness, and calm the nerves. They are used for abdominal distension and pain, indigestion, diarrhea and dysentery, rheumatic pain, lower back and knee weakness, and insomnia.

[0025] Star anise is the dried mature fruit of Illicium verum Hook. f. of Magnoliaceae. The fruit is picked when it changes from green to yellow in autumn and winter, and then dried after being slightly boiled in boiling water or directly dried. It is pungent and warm, and belongs to the meridians of the liver, kidney, spleen and stomach. It can warm yang and dispel cold, regulate qi and relieve pain. It is used for cold abdominal pain due to abdominal mass, lumbago due to kidney deficiency, vomiting due to stomach cold, and cold abdominal pain.

[0026] Sanchi is the dried root and rhizome of Panax notoginseng (Burk.) F. H. Chen of Araliaceae. It is dug before flowering in autumn, washed, separated into main roots, branch roots and rhizomes, and dried. It is sweet and slightly bitter, and warm. It belongs to the meridians of the liver and stomach. It can stop bleeding by dispersing blood stasis, and relieve pain by resolving swelling. It is used for hemoptysis, hematemesis, metrorrhagia, uterine bleeding, blood in stool, postpartum blood stasis, and chest and abdominal pain due to trauma.

[0027] Hawthorn is the dried mature fruit of Crataegus pinnatifida Bge. var. major N. E. Br. or Crataegus pinnatifida Bge. of Rosaceae. It is harvested when the fruit is mature in autumn, sliced and dried. It is sour, sweet and slightly warm. It belongs to the meridians of the spleen, stomach and liver. It can digest food, invigorate the stomach, disperse blood stasis, resolve turbidity and lower lipid. It is used for meat accumulation and stagnation, abdominal fullness, dysentery and abdominal pain, blood stasis and amenorrhea, postpartum blood stasis, chest and abdominal pain, angina pectoris, and hyperlipidemia. Foced hawthorn can enhance the effect of digesting food and removing stagnation. It is used for meat accumulation and stagnation, and dysentery.

[0028] Cloves are the dried flower buds of Eugenia caryophyllata Thunb. of Myrtaceae. They are picked when the flower buds change from green to red, and then dried. They are pungent and warm, and belong to the meridians of the spleen, stomach, lung and kidney. They can warm the middle and lower the adverse flow of qi, and tonify the kidney and assist yang. They are used for spleen and stomach deficiency and cold, hiccup and vomiting, poor appetite and diarrhea, cold abdominal pain, and kidney deficiency and impotence.

[0029] By using the above technical scheme, due to the reasonable matching and effective extraction method of the Portulaca oleracea, Chaenomelis fruit, Polygonatum, Cynanchum, star anise, sanchi, hawthorn and Pu'er tea, the composition has a significant effect of lowering blood lipid and blood sugar. The addition of cloves in the composition enhances the effect of lowering blood lipid and blood sugar. BRIEF DESCRIPTION OF DRAWINGS

[0030] Figure 1 TC, TG, LDL-C and HDL-C changes of rats after modeling of hyperlipidemia model;

[0031] Figure 2 TC, TG, LDL-C and HDL-C changes of rats after modeling of hyperlipidemia model;

[0032] Figure 3 The hypoglycemic effect of the composition obtained in each embodiment on normal rats;

[0033] Figure 4 The influence of the composition obtained in each embodiment on the blood glucose of hyperglycemic model rats. DETAILED DESCRIPTION

[0034] The specific embodiments of the present application will be further described below with reference to the drawings. It should be noted that the description of these embodiments is used to help understand the present application, but does not constitute a limitation on the present application. In addition, the technical features involved in each embodiment of the present application described below can be combined with each other as long as they do not conflict with each other.

[0035] Example 1

[0036] The present embodiment provides a composition for treating hyperlipidemia and hyperglycemia, which is composed of the following raw materials in parts by weight: 28 parts of Portulaca oleracea, 10 parts of Chaenomeles speciosa, 12 parts of Polygonatum sibiricum, 10 parts of Valeriana jatamansi, 0.5 parts of Illicium verum, 0.5 parts of Panax notoginseng, 5 parts of Crataegus pinnatifida and 8 parts of Pu'er tea.

[0037] The preparation method comprises the following steps:

[0038] S1, the above raw materials are weighed in parts by weight;

[0039] S2, the crushed Portulaca oleracea, Chaenomeles speciosa, Illicium verum, Crataegus pinnatifida and Pu'er tea are put into a container, water is added, and decoction is performed, and the first medicinal liquid and the first medicinal residue are obtained by filtration; the first medicinal residue is put into the container again, water is added, and decoction is performed, and the second medicinal liquid and the second medicinal residue are obtained by filtration; the first medicinal liquid and the second medicinal liquid are combined, and then distilled under reduced pressure, concentrated and dried, and then crushed to obtain the first extract;

[0040] S3, the crushed Valeriana jatamansi and Polygonatum sibiricum are put into a reflux extraction device, 90% Vol ethanol is added, and reflux extraction is performed at 72°C for 2 hours, and the third medicinal residue and the third medicinal liquid are obtained by filtration; the third medicinal liquid is distilled under reduced pressure, concentrated and dried to obtain the second extract; the third medicinal residue is put into the reflux extraction device, ethyl acetate is added, and reflux extraction is performed at 68°C for 1 hour, and the fourth medicinal liquid and the fourth medicinal residue are obtained by filtration; the fourth medicinal liquid is concentrated and dried under reduced pressure, and then crushed to obtain the third extract;

[0041] S4, the crushed Panax notoginseng, the first extract, the second extract and the third extract are combined and uniformly mixed to obtain the composition for treating hyperlipidemia and hyperglycemia.

[0042] Example 2

[0043] The embodiment provides a composition for treating hyperlipidemia and hyperglycemia, which is composed of the following raw materials in parts by weight: 10 parts of spilanthes acmella, 25 parts of chaenomeles speciosa, 5 parts of polygonatum sibiricum, 5 parts of daphne genkwa, 3 parts of illicium verum, 4 parts of panax notoginseng, 1 part of crataegus pinnatifida, and 2 parts of pu'er tea.

[0044] The preparation method comprises the following steps:

[0045] S1, the above raw materials are weighed according to weight parts;

[0046] S2, the crushed spilanthes acmella, chaenomeles speciosa, illicium verum, crataegus pinnatifida and pu'er tea are put into a container, water is added, decoction is carried out, and first medicinal liquid and first medicinal residue are obtained through filtration; the first medicinal residue is put into the container again, water is added, decoction is carried out, and second medicinal liquid and second medicinal residue are obtained through filtration; the first medicinal liquid and the second medicinal liquid are combined, vacuum distillation is carried out, and the first extract is obtained through concentration and dryness, crushing and the like.

[0047] S3, the crushed daphne genkwa and polygonatum sibiricum are put into a reflux extraction device, 75% Vol ethanol is added, and reflux extraction is carried out at 65 DEG C for 2 hours, and third medicinal residue and third medicinal liquid are obtained through filtration; the third medicinal liquid is vacuum distilled and concentrated and dried, and the second extract is obtained; the third medicinal residue is put into the reflux extraction device, ethyl acetate is added, and reflux extraction is carried out at 75 DEG C for 1 hour, and fourth medicinal liquid and fourth medicinal residue are obtained through filtration; the fourth medicinal liquid is vacuum concentrated and dried, and the third extract is obtained through crushing and the like.

[0048] S4, the crushed panax notoginseng, the first extract, the second extract and the third extract are combined and uniformly mixed, and the composition for treating hyperlipidemia and hyperglycemia is obtained.

[0049] Embodiment 3

[0050] The embodiment provides a composition for treating hyperlipidemia and hyperglycemia, which is composed of the following raw materials in parts by weight: 15 parts of spilanthes acmella, 20 parts of chaenomeles speciosa, 5 parts of polygonatum sibiricum, 8 parts of daphne genkwa, 2 parts of illicium verum, 3 parts of panax notoginseng, 5 parts of crataegus pinnatifida and 2 parts of pu'er tea.

[0051] The preparation method comprises the following steps:

[0052] S1, the above raw materials are weighed according to weight parts;

[0053] S2, the crushed spilanthes acmella, chaenomeles speciosa, illicium verum, crataegus pinnatifida and pu'er tea are put into a container, water is added, decoction is carried out, and first medicinal liquid and first medicinal residue are obtained through filtration; the first medicinal residue is put into the container again, water is added, decoction is carried out, and second medicinal liquid and second medicinal residue are obtained through filtration; the first medicinal liquid and the second medicinal liquid are combined, vacuum distillation is carried out, and the first extract is obtained through concentration and dryness, crushing and the like.

[0054] S3, put the crushed spider and the rhizoma polygonati into a reflux extraction device, add 82% Vol ethanol, heat at 71°C to reflux extract for 2 hours, filter to obtain third dregs and third liquid, distill the third liquid under reduced pressure, concentrate and dry, to obtain the second extract; put the third dregs into the reflux extraction device, add ethyl acetate, heat at 72°C to reflux extract for 1 hour, filter to obtain fourth liquid and fourth dregs; concentrate and dry the fourth liquid under reduced pressure, crush to obtain the third extract;

[0055] S4, combine and mix the crushed panax notoginseng, the first extract, the second extract and the third extract evenly, to obtain the composition for treating hyperlipidemia and hyperglycemia.

[0056] Example 4

[0057] The embodiment provides a composition for treating hyperlipidemia and hyperglycemia, which is composed of the following raw materials in parts by weight: 22 parts of the portulaca oleracea, 13 parts of the chaenomeles speciosa, 8 parts of the rhizoma polygonati, 5 parts of the daphne giraldii, 1 part of the illicium verum, 1 part of the panax notoginseng, 3 parts of the crataegus pinnatifida and 5 parts of the pu'er tea.

[0058] The preparation method comprises the following steps:

[0059] S1, weigh the above raw materials according to the weight fraction;

[0060] S2, put the crushed portulaca oleracea, chaenomeles speciosa, illicium verum, crataegus pinnatifida and pu'er tea into a container, add water, decoct, filter to obtain first liquid and first dregs; put the first dregs into the container again, add water, decoct, filter to obtain second liquid and second dregs; combine the first liquid and the second liquid, distill under reduced pressure, concentrate and dry, crush to obtain the first extract;

[0061] S3, put the crushed spider and the rhizoma polygonati into a reflux extraction device, add 82% Vol ethanol, heat at 71°C to reflux extract for 2 hours, filter to obtain third dregs and third liquid, distill the third liquid under reduced pressure, concentrate and dry, to obtain the second extract; put the third dregs into the reflux extraction device, add ethyl acetate, heat at 72°C to reflux extract for 1 hour, filter to obtain fourth liquid and fourth dregs; concentrate and dry the fourth liquid under reduced pressure, crush to obtain the third extract;

[0062] S4, combine and mix the crushed panax notoginseng, the first extract, the second extract and the third extract evenly, to obtain the composition for treating hyperlipidemia and hyperglycemia.

[0063] Example 5

[0064] The embodiment provides a composition for treating hyperlipidemia and hyperglycemia, which is composed of the following raw materials in parts by weight: 20 parts of spilanthes acmella, 16 parts of chaenomeles speciosa, 7 parts of polygonatum sibiricum, 7 parts of diterpine, 1 part of star anise, 2 parts of panax notoginseng, 4 parts of hawthorn, and 2 parts of pu'er tea.

[0065] The preparation method comprises the following steps:

[0066] S1, the raw materials are weighed according to the weight fraction;

[0067] S2, the crushed spilanthes acmella, chaenomeles speciosa, star anise, hawthorn and pu'er tea are put into a container, water is added, decoction is performed, and first medicinal liquid and first medicinal residue are obtained through filtration; the first medicinal residue is put into the container again, water is added, decoction is performed, and second medicinal liquid and second medicinal residue are obtained through filtration; the first medicinal liquid and the second medicinal liquid are combined, vacuum distillation is performed, and the first extract is obtained through concentration and dryness, crushing, and the like.

[0068] S3, the crushed diterpine and polygonatum sibiricum are put into a reflux extraction device, 85% Vol ethanol is added, and reflux extraction is performed at 70 DEG C for 2 hours; the third medicinal residue and the third medicinal liquid are obtained through filtration; the third medicinal liquid is vacuum distilled and concentrated and dried; the second extract is obtained; the third medicinal residue is put into the reflux extraction device, ethyl acetate is added, and reflux extraction is performed at 69 DEG C for 1 hour; the fourth medicinal liquid and the fourth medicinal residue are obtained through filtration; the fourth medicinal liquid is vacuum concentrated and dried, and the third extract is obtained through crushing.

[0069] S4, the crushed panax notoginseng, the first extract, the second extract and the third extract are combined and uniformly mixed to obtain the composition for treating hyperlipidemia and hyperglycemia.

[0070] Embodiment 6

[0071] The embodiment provides a composition for treating hyperlipidemia and hyperglycemia, which is composed of the following raw materials in parts by weight: 20 parts of spilanthes acmella, 16 parts of chaenomeles speciosa, 7 parts of polygonatum sibiricum, 7 parts of diterpine, 1 part of star anise, 2 parts of panax notoginseng, 4 parts of hawthorn, 2 parts of pu'er tea and 4 parts of clove.

[0072] The preparation method comprises the following steps:

[0073] S1, the raw materials are weighed according to the weight fraction;

[0074] S2, the crushed spilanthes acmella, chaenomeles speciosa, star anise, hawthorn, pu'er tea and clove are put into a container, water is added, decoction is performed, and first medicinal liquid and first medicinal residue are obtained through filtration; the first medicinal residue is put into the container again, water is added, decoction is performed, and second medicinal liquid and second medicinal residue are obtained through filtration; the first medicinal liquid and the second medicinal liquid are combined, vacuum distillation is performed, and the first extract is obtained through concentration and dryness, crushing, and the like.

[0075] S3. Add the crushed spider venom and polygonatum to a reflux extraction apparatus, add 85% ethanol, heat to reflux at 70°C for 2 hours, filter to obtain the third residue and the third liquid, distill the third liquid under reduced pressure, concentrate and dry to obtain the second extract; add the third residue to a reflux extraction apparatus, add ethyl acetate, heat to reflux at 69°C for 1 hour, filter to obtain the fourth liquid and the fourth residue; concentrate the fourth liquid under reduced pressure, dry, crush to obtain the third extract;

[0076] S4. Combine and mix the pulverized Panax notoginseng, the first extract, the second extract and the third extract evenly to obtain a composition for treating hyperlipidemia and hyperglycemia.

[0077] Comparative Example 1

[0078] This comparative example provides a composition, by weight, consisting of the following ingredients: 20 parts purslane, 16 parts papaya, 7 parts spider lily, 1 part star anise, 2 parts Panax notoginseng, 4 parts hawthorn, and 2 parts Pu-erh tea.

[0079] Its preparation method includes the following steps:

[0080] S1. Weigh the above raw materials according to the weight proportions;

[0081] S2. Put the crushed purslane, papaya, star anise, hawthorn and Pu-erh tea into a container, add water, decoct, filter to obtain the first decoction and the first dregs; put the first dregs into the container again, add water, decoct, filter to obtain the second decoction and the second dregs; combine the first decoction and the second decoction, distill under reduced pressure, concentrate and dry, crush to obtain the first extract.

[0082] S3. The crushed spider venom was placed into a reflux extraction device, 85% ethanol was added, and the mixture was heated to reflux at 70°C for 2 hours. The residue and the liquid were filtered to obtain the third residue and the third liquid. The liquid was distilled under reduced pressure and concentrated to dryness to obtain the second extract. The residue was placed into a reflux extraction device, ethyl acetate was added, and the mixture was heated to reflux at 69°C for 1 hour. The mixture was filtered to obtain the fourth liquid and the fourth residue. The liquid was concentrated under reduced pressure and dried to dryness, and then crushed to obtain the third extract.

[0083] S4. Combine and mix the pulverized Panax notoginseng, the first extract, the second extract and the third extract evenly to obtain the composition.

[0084] Comparative Example 2

[0085] This comparative example provides a composition, by weight, consisting of the following ingredients: 20 parts purslane, 16 parts papaya, 7 parts polygonatum, 1 part star anise, 2 parts notoginseng, 4 parts hawthorn, and 2 parts Pu-erh tea.

[0086] Its preparation method includes the following steps:

[0087] S1, by weight, take the above raw materials;

[0088] S2, the crushed purslane, papaya, star anise, hawthorn and Pu'er tea into the container, add water, decoction, filtration of the first drug and the first drug residue; the first drug residue is put into the container again, add water, decoction, filtration of the second drug and the second drug residue; the first drug and the second drug liquid is combined, reduced pressure distillation, concentrated dry, crushing, get the first extract;

[0089] S3, the crushed Huangjing into the reflux extraction device, add 85% Vol ethanol, heated to 65-72 ℃ reflux extraction 2 hours, filtration of the third drug residue and the third drug liquid, the third drug liquid reduced pressure distillation, concentrated dry, get the second extract; the third drug residue into the reflux extraction device, add ethyl acetate, heated to 68-75 ℃ reflux extraction 1 hour, filtration, get the fourth drug liquid and the fourth drug residue; the fourth drug liquid reduced pressure concentration dry, crushing, get the third extract;

[0090] S4, the crushed Panax notoginseng, the first extract, the second extract and the third extract are combined, mixed evenly, the composition is obtained.

[0091] Test example

[0092] 1, the effect of lowering blood lipids test

[0093] Test animals: SPF rats, male, 200±20g / one, a total of 100.

[0094] The rats were adaptively fed with maintenance feed for 5 days, and then the rats were randomly divided into 2 groups, one group of 10 rats as blank control group, the other group of 90 rats as model group. The blank control group was given maintenance feed, and the model group was given model feed (20.0% sucrose, 15.0% lard, 1.2% cholesterol and 0.2% sodium cholate were added in the maintenance feed). After 2 weeks of feeding, the tail blood was collected without fasting, and the serum was separated after blood collection. The serum TC, TG, LDL-C and HDL-C levels were determined by using the kit. Compared with the blank control group, the model group had significant differences in TC, TG and LDL-C, and the modeling was successful. The results are shown in Table 1 and Figure 1 .

[0095] Table 1 Change of TC, TG, LDL-C and HDL-C of rats after modeling ±s)

[0096]

[0097] From Table 1 and Figure 1It can be seen that, compared with the blank control group after modeling, TC, TG and LDL-C are significantly increased, which indicates that the modeling is successful.

[0098] After the modeling is successful, the model group is randomly divided into 9 groups, each group of 10, namely the model control group, the example 1 group, the example 2 group, the example 3 group, the example 4 group, the example 5 group, the example 6 group, the comparative example 1 group and the comparative example 2 group. After the 9 model groups are grouped, there is no significant difference in TC, TG, LDL-C and HDL-C between each group; the blank control group continues to be given the maintenance feed, and the model group continues to be given the model feed (20.0% sucrose, 15.0% lard, 1.2% cholesterol and 0.2% sodium cholate are added in the maintenance feed).

[0099] Dosing: the blank control group and the model control group are given normal saline 2mL, once a day, for 30 days. The example 1 group, the example 2 group, the example 3 group, the example 4 group, the example 5 group, the example 6 group, the comparative example 1 group and the comparative example 2 group are respectively given the composition 40mg obtained by the example 1, the example 2, the example 3, the example 4, the example 5, the example 6, the comparative example 1 and the comparative example 2, which is dissolved in normal saline and given by gavage for 30 days, once a day. At the end of the test, the tail blood is taken without fasting, the serum is separated after blood sampling, and the serum TC, TG, LDL-C and HDL-C levels are determined by using the kit. The results are shown in Table 2 and Figure 2 .

[0100] Table 2. The influence of the composition obtained by each example on the TC, TG, LDL-C and HDL-C of rats ±s, n=10

[0101]

[0102] It can be seen from Table 2 and Figure 2 Compared with the model control group, the TC, TG and LDL-C contents of the example 1 to 6 groups, the comparative example 1 group and the comparative example 2 group are significantly decreased, and the TC, TG and LDL-C contents of the comparative example 1 and 2 groups are higher than those of the example 1 to 6 groups. It can be seen that the composition obtained by the example 1 to 6 has a significant effect on reducing the TC, TG and LDL-C contents.

[0103] 2, Hypoglycemic effect test

[0104] 2.1 Normal rat hypoglycemic test

[0105] Test animals: SPF rats, male, 180±20g / each, a total of 90.

[0106] The rats were adaptively fed with maintenance feed for 5 days, and randomly divided into blank control group, Example 1 group, Example 2 group, Example 3 group, Example 4 group, Example 5 group, Example 6 group, Comparative Example 1 group and Comparative Example 2 group, 10 rats in each group. The rats were fasted for 3-5 hours, and tail blood was collected, and blood glucose was measured by blood glucose meter. The Example 1 group, Example 2 group, Example 3 group, Example 4 group, Example 5 group, Example 6 group, Comparative Example 1 group and Comparative Example 2 group were respectively given the composition obtained in Example 1, Example 2, Example 3, Example 4, Example 5, Example 6, Comparative Example 1 and Comparative Example 2, 40 mg, which was dissolved in normal saline and then orally administered for 30 days, once a day. The rats were fasted for 3-5 hours, and tail blood was collected, and blood glucose was measured by blood glucose meter. The results are shown in Table 3 and Figure 3 .

[0107] Table 3. Hypoglycemic effect of the composition obtained in each example on normal rats ±s, n=10

[0108]

[0109] It can be seen from Table 3 and Figure 3 that there is no significant difference between the initial blood glucose value and the blood glucose value after 30 days of administration in each group.

[0110] 2.2 Hypoglycemic test of islet injury hyperglycemia model

[0111] Test animals: SPF grade rats, male, 180±20 g each, a total of 90.

[0112] The rats were adaptively fed with maintenance feed for 5 days, and fasted for 4 hours, and the fasting blood glucose was measured as the basic blood glucose value of the rats in this batch. Then the rats were fasted for 24 hours (free water), and injected with pyrimidines for modeling, 150 mg / kg BW. ip. After 7 days of fasting for 4 hours, the blood glucose was measured, and the blood glucose value of 10-25 mmol / L was the success of the hyperglycemia model. The results are shown in Table 4.

[0113] Table 4. Modeling of rats ±s, n=90

[0114]

[0115] It can be seen from Table 4 that the blood glucose of the rats on the 7th day of modeling was significantly higher than the basic blood glucose of the rats, which was 17.23±3.53 mmol / L, and the blood glucose value was between 10-25 mmol / L, and the hyperglycemia model was successful.

[0116] The rats were then randomly divided into 9 groups, 10 rats in each group, i.e. a model control group, an Example 1 group, an Example 2 group, an Example 3 group, an Example 4 group, an Example 5 group, an Example 6 group, a Comparative Example 1 group and a Comparative Example 2 group. The model control group was given normal saline, and the Example 1 group, the Example 2 group, the Example 3 group, the Example 4 group, the Example 5 group, the Example 6 group, the Comparative Example 1 group and the Comparative Example 2 group were respectively given the composition obtained in Example 1, Example 2, Example 3, Example 4, Example 5, Example 6, Comparative Example 1 and Comparative Example 2, 40 mg, which was dissolved in normal saline and administered by gavage once a day for 30 days. The rats were fasted for 3-5 hours, tail blood was collected, blood glucose was measured by a blood glucose meter, and the blood glucose reduction percentage was calculated. The results are shown in Table 5 and Figure 4 .

[0117] Table 5. Effects of the compositions obtained in the various examples on the blood glucose of hyperglycemic model rats ±s, n=10

[0118]

[0119] It can be seen from Table 5 and Figure 4 that the blood glucose values of the Example 1 to 6 groups, the Comparative Example 1 group and the Comparative Example 2 group were significantly lower than that of the model control group, the blood glucose reduction rates of the Example 1 to 6 groups were higher than 56.75%, and the blood glucose reduction rates of the Comparative Example 1 and 2 groups were significantly lower than those of the Example 1 to 6 groups. It can be seen that the compositions obtained in the Example 1 to 6 groups have a significant effect of reducing blood glucose.

[0120] The embodiments of the present application are described in detail above with reference to the drawings, but the present application is not limited to the described embodiments. For those skilled in the art, various changes, modifications, replacements and variations of the embodiments can be made without departing from the principles and spirits of the present application, and still fall within the protection scope of the present application.

Claims

1. A composition for treating hyperlipidemia, hyperglycemia, characterized by, By weight parts, consisting of the following raw materials: 10-28 parts of Portulaca oleracea, 10-25 parts of Chaenomelis, 5-12 parts of Polygonatum, 5-10 parts of Daphne, 0.5-3 parts of Illicium verum, 0.5-4 parts of Panax notoginseng, 1-5 parts of Crataegus pinnatifida and 2-8 parts of Pu'er tea.

2. The composition for treating hyperlipidemia and hyperglycemia according to claim 1, wherein By weight parts, consisting of the following raw materials: 15-22 parts of Portulaca oleracea, 13-20 parts of Chaenomelis, 5-8 parts of Polygonatum, 5-8 parts of Daphne, 1-2 parts of Illicium verum, 1-3 parts of Panax notoginseng, 3-5 parts of Crataegus pinnatifida and 2-5 parts of Pu'er tea.

3. The composition for treating hyperlipidemia and hyperglycemia according to claim 2, wherein By weight parts, consisting of the following raw materials: 20 parts of Portulaca oleracea, 16 parts of Chaenomelis, 7 parts of Polygonatum, 7 parts of Daphne, 1 part of Illicium verum, 2 parts of Panax notoginseng, 4 parts of Crataegus pinnatifida and 2 parts of Pu'er tea.

4. A composition for treating hyperlipidemia, hyperglycemia, characterized by, By weight parts, consisting of the following raw materials: 10-28 parts of Portulaca oleracea, 10-25 parts of Chaenomelis, 5-12 parts of Polygonatum, 5-10 parts of Daphne, 0.5-3 parts of Illicium verum, 0.5-4 parts of Panax notoginseng, 1-5 parts of Crataegus pinnatifida, 2-8 parts of Pu'er tea and 3-8 parts of Clove.

5. A method of preparing a composition for treating hyperlipidemia, hyperglycemia, characterized by, Comprising the following steps: S1, by weight parts, the following raw materials are weighed: 10-28 parts of Portulaca oleracea, 10-25 parts of Chaenomelis, 5-12 parts of Polygonatum, 5-10 parts of Daphne, 0.5-3 parts of Illicium verum, 0.5-4 parts of Panax notoginseng, 1-5 parts of Crataegus pinnatifida and 2-8 parts of Pu'er tea; S2, the crushed Portulaca oleracea, Chaenomelis, Illicium verum, Crataegus pinnatifida and Pu'er tea are put into a container, water is added, decoction is performed, and the first medicinal liquid and the first medicinal residue are obtained by filtration; the first medicinal residue is again put into a container, water is added, decoction is performed, and the second medicinal liquid and the second medicinal residue are obtained by filtration; the first medicinal liquid and the second medicinal liquid are combined, vacuum distillation is performed, concentrated and dried, crushed, and the first extract is obtained; S3, the crushed Daphne and Polygonatum are put into a reflux extraction device, 75-90% Vol ethanol is added, heated and reflux extracted for 2 hours, and the third medicinal residue and the third medicinal liquid are obtained by filtration; the third medicinal liquid is vacuum distilled, concentrated and dried, and the second extract is obtained; the third medicinal residue is put into a reflux extraction device, ethyl acetate is added, heated and reflux extracted for 1 hour, filtered, and the fourth medicinal liquid and the fourth medicinal residue are obtained; the fourth medicinal liquid is vacuum concentrated and dried, crushed, and the third extract is obtained; S4, the crushed Panax notoginseng, the first extract, the second extract and the third extract are combined and mixed uniformly, and the composition for treating hyperlipidemia and hyperglycemia is obtained.

6. The method for preparing the composition for treating hyperlipidemia and hyperglycemia according to claim 5, characterized in that, In the step S1, 3-8 parts of Clove are also weighed by weight parts and crushed.

7. The method for preparing the composition for treating hyperlipidemia and hyperglycemia according to claim 6, characterized in that, The crushed Clove is treated in the same way as the raw materials in step S2.

8. The method for preparing the composition for treating hyperlipidemia and hyperglycemia according to claim 7, characterized in that, The concentration of ethanol in step S3 is 85% Vol.

9. The method for preparing the composition for treating hyperlipidemia and hyperglycemia according to claim 8, characterized in that, The temperature for heating and reflux extraction of ethanol in step S3 is 65-72℃.

10. The method for preparing the composition for treating hyperlipidemia and hyperglycemia according to claim 9, characterized in that, The temperature for heating and reflux extraction of ethyl acetate in step S3 is 68-75℃.

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