An umbilical cord mesenchymal stem cell separation device

Abstract

The application provides an umbilical cord mesenchymal stem cell separation device, and belongs to the technical field of cell separation, comprising a support table, a support frame is fixedly connected to the support table, a top of the support frame is fixedly connected with an umbilical cord segmentation table for conveniently preprocessing the umbilical cord, a processing assembly is fixedly connected to the support table, a separation assembly for separating stem cells from umbilical cord Wharton's jelly is arranged in the processing assembly, and a cutting assembly for cutting the umbilical cord Wharton's jelly is fixedly connected to the processing assembly. The application is excellent in automation and continuity of operation process, and after the Wharton's jelly separated by the segmentation table is placed on the cutting table, a series of operations such as subsequent cutting, enzymolysis, shock mixing and centrifugation can be automatically completed under the cooperative operation of each component of the device without frequent manual intervention, so that the working efficiency is greatly improved, and the continuity and stability of the separation process are ensured.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of cell separation, in particular to a kind of umbilical cord mesenchymal stem cell separation device. BACKGROUND

[0002] Umbilical cord mesenchymal stem cells are a kind of multi-functional stem cells existing in the perivascular tissue of newborn umbilical cord Wharton's jelly and blood vessels, with self-renewal and multi-directional differentiation potential, showing great potential in the fields of regenerative medicine, immune regulation and anti-aging, umbilical cord mesenchymal stem cells can differentiate into bone cells, chondrocytes, adipocytes, myocardial cells and other tissue cells, providing cell source for repairing damaged organs, and the proliferation and differentiation ability is superior to stem cells from bone marrow, fat and other sources.

[0003] And to obtain umbilical cord mesenchymal stem cells, single cell suspension needs to be obtained by mechanical, enzyme digestion or tissue culture first, and then mesenchymal stem cells are obtained by umbilical cord mesenchymal medium, the commonly used methods for primary separation include micro-tissue block wall culture method, umbilical cord homogenate collagenase digestion method and improved collagenase digestion method.

[0004] At present, the separation of umbilical cord stem cells is mostly manual operation, since the separation process involves multiple steps, usually multiple devices need to be replaced for operation, and manual operation not only consumes time and effort, but also introduces errors due to the proficiency of operators, individual differences and other factors, making it difficult to ensure the consistency and stability of the separation results, and when replacing the device, the sample needs to be transferred between different devices, which not only increases the operation steps and time cost, but also greatly increases the risk of sample contamination. SUMMARY

[0005] The purpose of the present application is to provide a kind of umbilical cord mesenchymal stem cell separation device to solve the problems raised in the above background.

[0006] Therefore, the present application is as follows Technical scheme: a kind of umbilical cord mesenchymal stem cell separation device, including support table, the support table is fixedly connected with support frame, the top of the support frame is fixedly connected with umbilical cord segmentation table for conveniently pre-treating umbilical cord, the support table is fixedly connected with processing assembly, the processing assembly is provided with separation assembly for separating stem cells from umbilical cord Wharton's jelly, the processing assembly is also fixedly connected with cutting assembly for cutting umbilical cord Wharton's jelly, the discharge end of the cutting assembly is communicated with the processing assembly;

[0007] The separation assembly comprises a driving assembly for driving the cutting assembly, an oscillation assembly for promoting fusion of the enzyme solution and the Wharton's jelly tissue, and a centrifugal assembly for centrifugal separation of the stem cells in the processing assembly, the output end of the driving assembly is connected with the cutting assembly and the oscillation assembly respectively, the top of the processing assembly is further fixedly connected with a liquid adding assembly for inputting the enzyme solution and the centrifugal liquid into the processing assembly, the output end of the driving assembly is further fixedly connected with a conveying assembly, and the output end of the conveying assembly is connected with the liquid adding assembly.

[0008] Preferably, the processing assembly comprises a processing box, the top of the processing box is fixedly connected with a top cover, the cutting assembly is arranged on the top of the top cover, a plurality of groups of connecting grooves are arranged on the top of the top cover in a uniform distribution manner, a switching disc is further rotationally connected between the processing box and the top cover, a plurality of groups of rotating grooves are arranged on the switching disc, a processing cylinder is rotationally connected in each group of the rotating grooves, a centrifugal tube is slidably inserted in each group of the processing cylinders, an electric heating sheet for heating the solution in the centrifugal tube is fixedly connected in each group of the processing cylinders, and a tube cover is clamped on each group of the centrifugal tubes, a one-way valve plate is hinged in the tube cover, and a connecting hole is arranged on the bottom of each group of the processing cylinders.

[0009] The bottom of the switching disc is further provided with a rotating motor, and the output end of the rotating motor is fixedly connected with the bottom of the switching disc.

[0010] Preferably, the cutting assembly comprises a fixed disc, the top of the top cover is fixedly connected with a support plate for supporting rotation of the fixed disc, and the side of the fixed disc close to the support plate is fixedly connected with a first synchronous shaft.

[0011] Preferably, the side of the fixed disc away from the support plate is further fixedly connected with a first driving rod, a first driving frame is sleeved on the first driving rod, a cutting knife is fixedly connected to the bottom of the first driving frame, guide frames are fixedly connected to the two sides of the bottom of the first driving frame, and the bottoms of the two groups of guide frames penetrate through the top cover and extend into the processing box.

[0012] The cutting assembly further comprises a cutting table fixedly connected to the top cover, the cutting table is located directly below the cutting knife, a cutting groove matched with the cutting knife is arranged on the cutting table, a material guiding box is in communication with the bottom of the cutting table, the other end of the material guiding box extends into the processing box and is in communication with the adjacent centrifugal tube in the processing box, and a quantitative valve is arranged at the discharging end of the material guiding box.

[0013] Preferably, the driving assembly comprises a driving motor fixedly connected in the processing box, the driving motor is arranged on one side of the processing box close to the oscillation assembly, the output end of the driving motor is fixedly connected with a first bevel gear, one side of the first bevel gear close to the driving motor is fixedly connected with a second synchronous shaft, a synchronous belt is sleeved on the second synchronous shaft, and the other end of the synchronous belt penetrates to the outside of the processing box and is sleeved on the first synchronous shaft on the fixed disc; the second synchronous shaft is in transmission connection with the first synchronous shaft through the synchronous belt.

[0014] The driving assembly further comprises a second bevel gear, and the second bevel gear is in meshing connection with the first bevel gear; one side of the first bevel gear close to the second bevel gear is further fixedly connected with a synchronous rod, and the synchronous rod is fixed with the conveying assembly.

[0015] Preferably, the oscillation assembly comprises a fixing frame fixedly connected in the processing box, the second bevel gear is rotationally connected to the fixing frame, the fixing frame is rotationally connected with a transmission rod, one side of the transmission rod close to the fixing frame penetrates the fixing frame and is fixed with the second bevel gear, and the other end of the transmission rod is fixedly connected with a first transmission plate; one side of the first transmission plate away from the transmission rod is fixedly connected with a push rod.

[0016] Preferably, the oscillation assembly further comprises a rotating rod rotationally connected to the fixing frame, and the rotating rod is located directly below one group of connecting grooves; one side of the rotating rod away from the second bevel gear is located on the fixing frame; two groups of connecting frames are fixedly connected to the rotating rod; a driving plate is hingedly connected between the two groups of connecting frames, and one end of the driving plate away from the rotating rod is located on the connecting frame; a sliding groove is formed in one side of the driving plate away from the rotating rod; and one end of the push rod on the first transmission plate close to the sliding groove is slidably connected to the sliding groove.

[0017] The top of the rotating rod is further fixedly connected with a synchronous disc, the synchronous disc is rotationally connected to the top of the fixing frame, the top of the synchronous disc is fixedly connected with a first electric push rod, and the output end of the first electric push rod is fixedly connected with a plurality of positioning rods matched with the connecting holes.

[0018] Preferably, the centrifugal assembly is arranged directly below one group of connecting grooves, and the centrifugal assembly comprises two groups of second electric push rods, the two groups of second electric push rods are fixedly connected in the processing box, the output ends of the two groups of second electric push rods are fixedly connected with a connecting disc, the top of the connecting disc is rotationally connected with a centrifugal cylinder, and the inner wall of the bottom of the centrifugal cylinder is fixedly connected with a connecting rod matched with the connecting hole in the bottom of the processing cylinder.

[0019] The bottom of the connecting disc is further fixedly connected with a centrifugal motor, the output end of the centrifugal motor penetrates the connecting disc and is fixed with the centrifugal cylinder.

[0020] Preferably, the liquid adding assembly comprises a top lifting cylinder fixedly connected to the top cover, a first liquid adding frame and a second liquid adding frame fixedly connected to the output end of the top lifting cylinder, the first liquid adding frame and the second liquid adding frame are located directly above the driving assembly and the centrifugal assembly respectively, the bottom of each of the first liquid adding frame and the second liquid adding frame is fixedly connected with a liquid adding pipe, the first liquid adding frame and the second liquid adding frame are respectively connected with adjacent liquid adding pipes, the second liquid adding frame is further connected with a liquid feeding pipe, the other end of the liquid feeding pipe is connected with the enzyme solution storage tank.

[0021] The first liquid adding frame is further connected with a communication pipe, the other end of the communication pipe extends into the treatment tank and is connected with the conveying assembly.

[0022] Preferably, the conveying assembly comprises a second transmission plate and a conveying piston, one end of the second transmission plate is fixedly connected with the synchronization rod, the other end of the second transmission plate is fixedly connected with a second driving rod, the second driving rod is sleeved with a second driving frame, the side of the second driving frame close to the second transmission plate is fixedly connected with a driving disc, the conveying piston is fixedly connected in the treatment tank, the conveying piston is slidably connected with a piston rod, the end of the driving disc away from the second driving frame is fixedly connected with the top of the piston rod in the conveying piston, the end of the second driving frame close to the inner wall of the treatment tank is further fixedly connected with a guide rod, the inner wall of the treatment tank is provided with a guide groove for the guide rod to slide.

[0023] The feeding end of the conveying piston is further connected with a conveying pipe, the other end of the conveying pipe penetrates through the treatment tank and extends to the outside of the treatment tank, the extending end of the conveying pipe is connected with a centrifugal liquid storage tank, the discharging end of the conveying pipe is connected with the communication pipe, the feeding end and the discharging end of the conveying pipe are both provided with a one-way valve.

[0024] Compared with the prior art, the beneficial effects of the present application include:

[0025] The device integrates multiple steps such as umbilical cord processing, Wharton's jelly cutting, solution adding, oscillation mixing and centrifugal separation in one system. After the umbilical cord is cut at the umbilical cord cutting table, subsequent cutting, quantitative dropping into the centrifugal tube, liquid adding, oscillation mixing, centrifugal separation and other operations are automatically completed by the driving motor, the rotating motor and the electric push rod. This automatic design reduces the errors and time consumption caused by manual operation, greatly improves the efficiency of separation work, and can process more umbilical cord samples in a short time to meet the needs of large-scale stem cell separation.

[0026] In the cutting link, the driving motor drives the first bevel gear to rotate, the up-down movement of the cutting knife is accurately controlled through the synchronization shaft and the synchronization belt, the precise cutting of Wharton's jelly is realized, and the uniform cutting size is ensured to provide a good foundation for subsequent separation.

[0027] The oscillation component, through a series of transmission structures including the first bevel gear driving the second bevel gear, causes the processing cylinder to rotate left and right, allowing the enzyme solution to mix thoroughly with Wharton's gum and improving enzymatic hydrolysis efficiency. The centrifugation component, through a centrifugal motor driving the centrifuge cylinder to rotate, achieves centrifugal separation and effectively separates stem cells. This multi-functional collaborative work avoids the transfer of samples between different devices, reduces the risk of contamination, and optimizes the separation process, making the entire separation process smoother and more efficient.

[0028] In addition, the device’s cyclic processing capability further enhances its practicality. The rotary motor drives the switching disk to rotate, allowing the processing cylinder to switch positions above different components, enabling continuous processing of multiple samples. While one sample is being mixed in the oscillation component, another sample can be centrifuged in the centrifugation component. Once one sample has been processed, the next sample can be quickly moved to the corresponding position for further processing, greatly shortening the overall processing time and improving the utilization rate of the device. Attached Figure Description

[0029] Figure 1 A first-view schematic diagram of the present invention is shown;

[0030] Figure 2 A second-view schematic diagram of the present invention is shown;

[0031] Figure 3 This invention illustrates the following: Figure 1 Enlarged schematic diagram of the structure at point A;

[0032] Figure 4 A partial structural diagram of the processing component in this invention is shown;

[0033] Figure 5 A cross-sectional view of the internal structure of the processing box in this invention is shown. Figure 1 ;

[0034] Figure 6 A cross-sectional view of the internal structure of the processing box in this invention is shown. Figure 2 ;

[0035] Figure 7 A schematic diagram of the structure of the driving component and the oscillation component in this invention is shown;

[0036] Figure 8 A schematic diagram of the oscillation component in this invention is shown;

[0037] Figure 9 This invention illustrates the following: Figure 2 Enlarged schematic diagram of the structure at point B above;

[0038] Figure 10 This invention illustrates the following: Figure 5 Enlarged schematic diagram of the structure at point C;

[0039] Figure 11 The top view of the present application is shown.

[0040] In the figure: 1, support table; 11, support frame; 12, umbilical cord segmentation table; 13, centrifugal liquid storage tank; 2, processing assembly; 21, processing tank; 22, top cover; 23, connecting groove; 24, switching disc; 25, rotating groove; 26, processing cylinder; 27, centrifugal tube; 28, tube cover; 29, rotary motor; 3, cutting assembly; 31, fixed disc; 32, first driving rod; 33, first driving frame; 34, cutting knife; 35, cutting table; 36, material guiding box; 37, guiding frame; 4, driving assembly; 41, driving motor; 42, first bevel gear; 43, second bevel gear; 44, synchronous belt; 45, synchronous rod; 5, oscillation assembly; 51, fixed frame; 52, transmission rod; 53, first transmission plate; 54, rotating rod; 55, connecting frame; 56, driving plate; 57, sliding groove; 58, first electric push rod; 59, positioning rod; 6, centrifugal assembly; 61, second electric push rod; 62, connecting disc; 63, centrifugal cylinder; 64, centrifugal motor; 7, liquid adding assembly; 71, top lifting air cylinder; 72, first liquid adding frame; 73, second liquid adding frame; 74, liquid adding pipe; 75, enzyme solution storage tank; 76, liquid feeding pipe; 77, communication pipe; 8, conveying assembly; 81, second transmission plate; 82, second driving rod; 83, second driving frame; 84, driving disc; 85, conveying piston; 86, conveying pipe. DETAILED DESCRIPTION

[0041] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present application.

[0042] Please refer to Figures 1 to 11The application provides a kind of umbilical cord mesenchymal stem cell separation device technical scheme: a kind of umbilical cord mesenchymal stem cell separation device, including support table 1, support table 1 is fixedly connected with support frame 11, the top of support frame 11 is fixedly connected with umbilical cord segmentation table 12 for conveniently preprocessing umbilical cord, processing assembly 2 is fixedly connected on support table 1, separation assembly for separating stem cells from umbilical cord wintone glue is provided in processing assembly 2, cutting assembly 3 for cutting umbilical cord wintone glue is also fixedly connected on processing assembly 2, the discharge end of cutting assembly 3 is communicated with processing assembly 2, separation assembly includes drive assembly 4 in processing assembly 2 for driving cutting assembly 3, oscillation assembly 5 for promoting enzyme solution and wintone glue tissue fusion and centrifugal assembly 6 for centrifugal separation of stem cells, the output end of drive assembly 4 is connected with cutting assembly 3 and oscillation assembly 5 respectively, the top of processing assembly 2 is also fixedly connected with liquid adding assembly 7 for inputting enzyme solution and centrifugal liquid into processing assembly 2, the output end of drive assembly 4 is also fixedly connected with conveying assembly 8, and the output end of conveying assembly 8 is communicated with liquid adding assembly 7;

[0043] Specifically, in use, first, after the sterilization treatment, umbilical cord is placed on umbilical cord segmentation table 12, umbilical cord is cut, blood vessels are separated from wintone glue in umbilical cord, after separation, wintone glue is placed on cutting assembly 3, drive assembly 4 is started, cutting assembly 3 is driven by drive assembly 4 to start cutting wintone glue, and the cut wintone glue falls into processing assembly 2 to separate stem cells.

[0044] As a kind of umbilical cord mesenchymal stem cell separation device optimization scheme, processing assembly 2 includes processing box 21, the top of processing box 21 is fixedly connected with top cover 22, cutting assembly 3 is arranged on the top of top cover 22, a plurality of groups of connection grooves 23 distributed uniformly are formed in the top of top cover 22, processing box 21 and top cover 22 are also rotatably connected with switching disc 24, a plurality of groups of rotating grooves 25 are formed in switching disc 24, processing cylinder 26 is rotatably connected in each rotating groove 25, centrifugal tube 27 is slidably inserted in each processing cylinder 26, electric heating sheet for heating solution in centrifugal tube 27 is also fixedly connected in each processing cylinder 26, pipe cover 28 is also clamped on each centrifugal tube 27, one-way valve piece is hinged in pipe cover 28, connecting hole is also formed in the bottom of each processing cylinder 26, rotary motor 29 is also arranged on the bottom of switching disc 24, and the output end of rotary motor 29 is fixed with the bottom of switching disc 24;

[0045] Specifically, in use, after the cutting assembly 3 finishes cutting the Wharton's jelly, the Wharton's jelly is first added into one of the centrifugal tubes 27 through the cutting assembly 3, the rotating motor 29 is started to drive the switching disc 24 to rotate, so that the centrifugal tube 27 containing the Wharton's jelly is rotated to the upper side of the oscillation assembly 5, the liquid adding assembly 7 is started to add enzyme solution, and the oscillation assembly 5 is started to be fixed with the processing barrel 26, and in this process, the cutting assembly 3 can be replaced and disinfected;

[0046] After the addition is completed, when the driving assembly 4 continues the next round of cutting work, the driving assembly 4 is started to drive the oscillation assembly 5 to reciprocate left and right at the same time, the oscillation assembly 5 drives the adjacent processing barrel 26 to reciprocate, so that the Wharton's jelly is mixed with the enzyme solution and fully reacts.

[0047] As a further optimization scheme, the cutting assembly 3 comprises a fixed disc 31, the top of the top cover 22 is fixedly connected with a support plate for supporting the rotation of the fixed disc 31, a first synchronous shaft is fixedly connected to one side of the fixed disc 31 close to the support plate, a first driving rod 32 is also fixedly connected to the side of the fixed disc 31 away from the support plate, a first driving frame 33 is sleeved on the first driving rod 32, a cutting knife 34 is fixedly connected to the bottom of the first driving frame 33, guide frames 37 are also fixedly connected to the bottom of the first driving frame 33 on both sides, and the bottoms of the two groups of guide frames 37 penetrate through the top cover 22 and extend into the processing box 21, the cutting assembly 3 further comprises a cutting table 35 fixedly connected to the top cover 22, the cutting table 35 is located directly below the cutting knife 34, and a cutting groove matched with the cutting knife 34 is formed in the cutting table 35, a material guide box 36 is communicated with the bottom of the cutting table 35, the other end of the material guide box 36 extends into the processing box 21 and is communicated with the adjacent centrifugal tube 27 in the processing box 21, and a quantitative valve is arranged at the discharging end of the material guide box 36.

[0048] Specifically, when the Wharton's jelly is cut, the Wharton's jelly is first placed on the cutting table 35, when the driving assembly 4 is started, the driving assembly 4 drives the fixed disc 31 to rotate at the same time, the fixed disc 31 drives the first driving frame 33 to move up and down through the first driving rod 32, so that the cutting knife 34 moves up and down to cut the Wharton's jelly placed on the cutting table 35, and the cut Wharton's jelly falls into the material guide box 36 and is quantitatively dropped into the centrifugal tube 27 through the material guide box 36.

[0049] As a further optimization scheme, the driving assembly 4 comprises a driving motor 41 fixedly connected in the processing box 21, the driving motor 41 is arranged in the processing box 21 close to one side of the oscillation assembly 5, the output end of the driving motor 41 is fixedly connected with a first bevel gear 42, the first bevel gear 42 is fixedly connected with a second synchronous shaft close to one side of the driving motor 41, and the second synchronous shaft is sleeved with a synchronous belt 44, the other end of the synchronous belt 44 penetrates to the outside of the processing box 21 and is sleeved on the first synchronous shaft on the fixed disc 31, the second synchronous shaft is in transmission connection with the first synchronous shaft through the synchronous belt 44, and the driving assembly 4 further comprises a second bevel gear 43, and the second bevel gear 43 is in mesh with the first bevel gear 42, and the first bevel gear 42 is further fixedly connected with a synchronous rod 45 close to one side of the second bevel gear 43, and the synchronous rod 45 is fixed with the conveying assembly 8;

[0050] Specifically, in use, first, the driving motor 41 is started, so that the driving motor 41 drives the first bevel gear 42 to rotate, when the first bevel gear 42 rotates, the first bevel gear 42 first drives the first synchronous shaft on the fixed disc 31 to rotate through the second synchronous shaft and the synchronous belt 44 thereon, so that the fixed disc 31 rotates to drive the cutting knife 34 to cut;

[0051] When the first bevel gear 42 rotates, the first bevel gear 42 drives the second bevel gear 43 to rotate at the same time, so that the second bevel gear 43 drives the oscillation assembly 5 to reciprocate, so that the enzyme solution and the Wharton's jelly fully contact and react.

[0052] As a further optimization scheme, the oscillation assembly 5 comprises a fixed frame 51 fixedly connected in the processing box 21, the second bevel gear 43 is rotatably connected to the fixed frame 51, the fixed frame 51 is rotatably connected with a transmission rod 52, and the transmission rod 52 penetrates through the fixed frame 51 close to one side of the fixed frame 51 and is fixed with the second bevel gear 43, the other end of the transmission rod 52 is fixedly connected with a first transmission plate 53, and the side, away from the transmission rod 52, of the first transmission plate 53 is fixedly connected with a push rod, the oscillation assembly 5 further comprises a rotating rod 54 rotatably connected to the fixed frame 51, and the rotating rod 54 is located directly below one group of connecting grooves 23, the rotating rod 54 is located on the side, away from the second bevel gear 43, of the fixed frame 51, the rotating rod 54 is fixedly connected with two groups of connecting frames 55, and a driving plate 56 is hingedly connected between the two groups of connecting frames 55, and the driving plate 56 is located at one end, away from the rotating rod 54, of the connecting frame 55, the side, away from the rotating rod 54, of the driving plate 56 is further provided with a sliding groove 57, and the end, close to the sliding groove 57, of the push rod on the first transmission plate 53 is slidably connected to the sliding groove 57, and the top of the rotating rod 54 is further fixedly connected with a synchronous disc, the synchronous disc is rotatably connected to the top of the fixed frame 51, the top of the synchronous disc is fixedly connected with a first electric push rod 58, and the output end of the first electric push rod 58 is fixedly connected with a plurality of positioning rods 59 matched with the connecting holes;

[0053] Specifically, after the centrifuge tube 27 with the Wharton's jelly is rotated to the upper side of the oscillation assembly 5, first, the tube cover 28 is covered on the centrifuge tube 27 to seal the centrifuge tube 27, the first electric push rod 58 is started to drive the positioning rod 59 to be inserted into the connecting hole at the bottom of the processing cylinder 26, the oscillation assembly 5 is fixed with the processing cylinder 26, after the fixation, the enzyme solution is added into the centrifuge tube 27 by the liquid adding assembly 7;

[0054] When the second bevel gear 43 rotates, the second bevel gear 43 drives the first transmission plate 53 to rotate through the transmission rod 52, when the first transmission plate 53 rotates, the push rod on the first transmission plate 53 starts to slide in the sliding groove 57 on the driving plate 56, under the driving of the first transmission plate 53 and the push rod, the driving plate 56 starts to drive the connecting frame 55 to rotate left and right, the connecting frame 55 drives the rotating rod 54 to rotate left and right at the same time, the rotating rod 54 drives the processing cylinder 26 to rotate left and right through the first electric push rod 58 and the positioning rod 59, so that the enzyme solution in the centrifuge tube 27 can be fully mixed with the Wharton's jelly;

[0055] After the mixing, the first electric push rod 58 is started again to drive the positioning rod 59 to be withdrawn, the rotating motor 29 is rotated again to move the centrifuge tube 27 after the full reaction to the upper side of the centrifugal assembly 6, at the same time, the centrifuge tube 27 with the Wharton's jelly is moved to the oscillation assembly 5 again under the driving of the processing cylinder 26, the above operation is repeated to continuously perform the separation work.

[0056] As a further optimization scheme, the centrifugal assembly 6 is arranged below one group of connecting grooves 23, the centrifugal assembly 6 includes two groups of second electric push rods 61, the two groups of second electric push rods 61 are fixedly connected in the processing box 21, the output ends of the two groups of second electric push rods 61 are fixedly connected with a connecting disc 62, the top of the connecting disc 62 is rotatably connected with a centrifugal cylinder 63, the inner wall at the bottom of the centrifugal cylinder 63 is fixedly connected with a connecting rod matched with the connecting hole at the bottom of the processing cylinder 26, the bottom of the connecting disc 62 is further fixedly connected with a centrifugal motor 64, the output end of the centrifugal motor 64 penetrates through the connecting disc 62 and is fixed with the centrifugal cylinder 63;

[0057] Specifically, when the centrifuge tube 27 after the mixing of the oscillation assembly 5 is moved to the centrifugal assembly 6, the two groups of second electric push rods 61 are started to drive the connecting disc 62, the centrifugal cylinder 63 and the centrifugal motor 64 to move upwards, so that the centrifugal cylinder 63 is sleeved on the bottom of the processing cylinder 26, at the same time, the connecting rod in the centrifugal cylinder 63 is inserted into the connecting hole at the bottom of the processing cylinder 26, at the same time, the centrifugal liquid is added into the centrifuge tube 27 by the liquid adding assembly 7;

[0058] After the fixation, the centrifugal motor 64 is started to drive the centrifugal cylinder 63 to rotate, the centrifugal cylinder 63 drives the processing cylinder 26 to rotate through the connecting rod, and then the centrifuge tube 27 is rotated to perform the centrifugal work;

[0059] When the centrifugal work is completed, the two groups of second electric push rods 61 drive the connecting disc 62 and the centrifugal cylinder 63 to move downward, so that the centrifugal cylinder 63 is separated from the processing cylinder 26, at this time, the rotating motor 29 rotates again to move the processing cylinder 26 away from the centrifugal assembly 6, and move the processing cylinder 26 at the shock assembly 5 to the centrifugal assembly 6 again to continue the work;

[0060] The centrifuged centrifugal tube 27 is taken out through the connecting groove 23, the supernatant is removed, the bottom precipitate is filtered to remove undigested tissues, and subsequent inoculation and culture work can be carried out.

[0061] As a further optimization scheme, the liquid adding assembly 7 includes a top lifting cylinder 71 fixedly connected to the top cover 22 and an enzyme solution storage tank 75, a first liquid adding frame 72 and a second liquid adding frame 73 are fixedly connected to the output end of the top lifting cylinder 71, the first liquid adding frame 72 and the second liquid adding frame 73 are located directly above the driving assembly 4 and the centrifugal assembly 6 respectively, the bottom of the first liquid adding frame 72 and the second liquid adding frame 73 is fixedly connected with a liquid adding pipe 74, and the first liquid adding frame 72 and the second liquid adding frame 73 are respectively connected with adjacent liquid adding pipes 74, the second liquid adding frame 73 is also connected with a liquid sending pipe 76, the other end of the liquid sending pipe 76 is connected with the enzyme solution storage tank 75, the first liquid adding frame 72 is also connected with a connecting pipe 77, the other end of the connecting pipe 77 extends into the processing tank 21 and is connected with the conveying assembly 8, the conveying assembly 8 includes a second driving plate 81 and a conveying piston 85, one end of the second driving plate 81 is fixedly connected with the synchronous rod 45, the other end of the second driving plate 81 is fixedly connected with a second driving rod 82, the second driving rod 82 is sleeved with a second driving frame 83, the second driving frame 83 is fixedly connected with a driving disc 84 on the side close to the second driving plate 81, the conveying piston 85 is fixedly connected in the processing tank 21, a piston rod is slidably connected in the conveying piston 85, the end of the driving disc 84 away from the second driving frame 83 is fixedly connected with the top of the piston rod in the conveying piston 85, the end of the second driving frame 83 close to the inner wall of the processing tank 21 is also fixedly connected with a guide rod, a guide groove is formed in the inner wall of the processing tank 21 for the guide rod to slide, the feeding end of the conveying piston 85 is also connected with a conveying pipe 86, the other end of the conveying pipe 86 penetrates through the processing tank 21 and extends to the outside of the processing tank 21, the extending end of the conveying pipe 86 is connected with the centrifugal liquid storage tank 13, the feeding end and the discharging end of the conveying pipe 86 are both provided with a one-way valve;

[0062] Specifically, when the centrifuge tube 27 with Wharton's jelly is moved to the shaking assembly 5, the lifting cylinder 71 is started, the lifting cylinder 71 drives the first liquid adding frame 72 and the second liquid adding frame 73 to move downward, so that the liquid adding tube 74 presses the one-way valve piece on the tube cover 28 and is inserted into the centrifuge tube 27, the liquid adding tube 74 on the second liquid adding frame 73 injects the enzyme solution in the enzyme solution storage tank 75 into the centrifuge tube 27 above the shaking assembly 5, the liquid adding tube 74 on the first liquid adding frame 72 injects the centrifugal liquid into the centrifuge tube 27 above the centrifugal assembly 6, after the injection is completed, the lifting cylinder 71 is started to drive the two groups of liquid adding tubes 74 to be extracted from the centrifuge tube 27, the driving motor 41 is started again, the processing cylinder 26 is driven to rotate by the shaking assembly 5, so that the enzyme solution in the centrifuge tube 27 is mixed with the Wharton's jelly, and the centrifugal assembly 6 is started synchronously to perform the centrifugal work;

[0063] When the driving assembly 4 is started, the first bevel gear 42 drives the synchronous rod 45 to rotate at the same time, the synchronous rod 45 drives the second transmission plate 81 to rotate, the second transmission plate 81 drives the second driving frame 83 to move up and down through the second driving rod 82, when the second driving frame 83 moves up and down, the driving disc 84 drives the piston rod in the delivery piston 85 to perform the suction movement, when the suction movement is performed, the delivery piston 85 draws the centrifugal liquid in the centrifugal liquid storage tank 13 through the delivery tube 86 and delivers the centrifugal liquid to the first liquid adding frame 72 through the communication pipe 77, so that the subsequent liquid adding work is facilitated.

[0064] The working principle of the umbilical cord mesenchymal stem cell separation device is as follows:

[0065] In use, first, the sterilized umbilical cord is placed on the umbilical cord cutting table 12, the umbilical cord is cut, the blood vessels and the like are separated from the Wharton's jelly in the umbilical cord, after the separation, the Wharton's jelly is placed on the cutting table 35;

[0066] The driving motor 41 is started, the driving motor 41 drives the first bevel gear 42 to rotate, when the first bevel gear 42 rotates, the first bevel gear 42 first drives the first synchronous shaft on the fixed disc 31 to rotate through the second synchronous shaft and the synchronous belt 44 thereon, the fixed disc 31 drives the first driving frame 33 to move up and down through the first driving rod 32, so that the cutting knife 34 moves up and down to cut the Wharton's jelly placed on the cutting table 35, the cut Wharton's jelly falls into the guide box 36 and is quantitatively dropped into the centrifuge tube 27 through the guide box 36, after the addition is completed, the rotating motor 29 is started to drive the switching disc 24 to rotate, so that the processing cylinder 26 originally located below the guide box 36 is rotated to the upper side of the shaking assembly 5, and the processing cylinder 26 originally located above the shaking assembly 5 is rotated to the upper side of the centrifugal assembly 6;

[0067] After moving to the position, the rotating motor 29 is closed to fix the switching disc 24, the lifting cylinder 71 is started to drive the first liquid adding frame 72 and the second liquid adding frame 73 to move downward, so that the liquid adding pipe 74 presses the one-way valve piece on the pipe cover 28 and inserts into the centrifugal tube 27, the liquid adding pipe 74 on the second liquid adding frame 73 injects the enzyme solution in the enzyme solution storage tank 75 into the centrifugal tube 27 above the oscillation assembly 5, the liquid adding pipe 74 on the first liquid adding frame 72 injects the centrifugal liquid into the centrifugal tube 27 above the centrifugal assembly 6, after the injection is completed, the first electric push rod 58 is started to drive the positioning rod 59 to insert into the connecting hole at the bottom of the processing cylinder 26, so that the oscillation assembly 5 is fixed with the processing cylinder 26, at the same time, the two groups of second electric push rods 61 drive the connecting disc 62, the centrifugal cylinder 63 and the centrifugal motor 64 to move upward, so that the centrifugal cylinder 63 is sleeved at the bottom of the processing cylinder 26, at the same time, the connecting rod in the centrifugal cylinder 63 inserts into the connecting hole at the bottom of the processing cylinder 26, after being fixed, the driving motor 41 is started again, the cutting assembly 3 continues the cutting work under the driving of the first bevel gear 42;

[0068] When the first bevel gear 42 rotates, the first bevel gear 42 drives the second bevel gear 43 to rotate at the same time, the second bevel gear 43 drives the first transmission plate 53 to rotate at the same time through the transmission rod 52, the push rod on the first transmission plate 53 slides in the sliding groove 57 on the driving plate 56, under the pushing of the first transmission plate 53 and the push rod, the driving plate 56 starts to drive the connecting frame 55 to rotate left and right, the connecting frame 55 drives the rotating rod 54 to rotate left and right at the same time, the rotating rod 54 drives the processing cylinder 26 to rotate left and right through the first electric push rod 58 and the positioning rod 59, so that the enzyme solution in the centrifugal tube 27 can be fully mixed with the Wharton's jelly;

[0069] The centrifugal motor 64 is started synchronously to drive the centrifugal cylinder 63 to rotate, the centrifugal cylinder 63 drives the processing cylinder 26 to rotate at the same time through the connecting rod, and then the centrifugal tube 27 rotates to perform the centrifugal work;

[0070] When the driving motor 41 is started to drive the first bevel gear 42 to rotate, the first bevel gear 42 drives the synchronous rod 45 to rotate at the same time, the synchronous rod 45 drives the second transmission plate 81 to rotate, the second transmission plate 81 drives the second driving frame 83 to move up and down through the second driving rod 82, so that the driving disc 84 drives the piston rod in the delivery piston 85 to perform the suction movement, when the suction movement is performed, the delivery piston 85 draws out the centrifugal liquid in the centrifugal liquid storage tank 13 through the delivery pipe 86, and then the centrifugal liquid is delivered to the first liquid adding frame 72 through the communication pipe 77, so as to facilitate the subsequent liquid adding work;

[0071] When running to the predetermined time, the drive motor 41 and centrifugal motor 64 are closed, the first electric push rod 58 is started again to drive the positioning rod 59 to retract, and the two sets of second electric push rod 61 are started to drive the connecting disc 62 and the centrifugal cylinder 63 to move downward, so that the centrifugal cylinder 63 is separated from the processing cylinder 26, the rotating motor 29 is rotated again to move the centrifugal tube 27 after full reaction to the upper of the centrifugal assembly 6, at the same time, the centrifugal tube 27 placed with Wharton's jelly is moved to the shock assembly 5 again under the drive of the processing cylinder 26, the above operation is repeated to continue the separation work;

[0072] The centrifugal tube 27 after centrifugation is taken out through the connecting groove 23, the supernatant is removed, the bottom precipitate is filtered to remove undigested tissues, and then subsequent inoculation and culture work can be carried out.

[0073] Obviously, the described embodiments are only a part of the embodiments of the present application, not all the embodiments. Those skilled in the art will appreciate that many modifications and variations can be made to the embodiments described herein without departing from the spirit or scope of the present application. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor belong to the scope of protection of the present application.

Claims

1. An umbilical cord mesenchymal stem cell separation device, comprising a support platform (1), a support frame (11) fixedly connected to the support platform (1), and an umbilical cord splitting table (12) for facilitating pretreatment of the umbilical cord fixedly connected to the top of the support frame (11), characterized in that: A processing component (2) is fixedly connected to the support platform (1). The processing component (2) is provided with a separation component for separating stem cells from Wharton's jelly in the umbilical cord. A cutting component (3) for chopping Wharton's jelly in the umbilical cord is also fixedly connected to the processing component (2). The discharge end of the cutting component (3) is connected to the processing component (2). The separation component includes a driving component (4) for driving the cutting component (3) within the processing component (2), a shaking component (5) for promoting the fusion of enzyme solution and Wharton's jelly tissue, and a centrifugation component (6) for centrifuging and separating stem cells. The output end of the driving component (4) is connected to the cutting component (3) and the shaking component (5) respectively. The top of the processing component (2) is also fixedly connected to a liquid addition component (7) for inputting enzyme solution and centrifugal fluid into the processing component (2). The output end of the driving component (4) is also fixedly connected to a delivery component (8), and the output end of the delivery component (8) is connected to the liquid addition component (7). The cutting assembly (3) includes a fixed disk (31), and a support plate for supporting the rotation of the fixed disk (31) is fixedly connected to the top of the top cover (22). A first synchronous shaft is fixedly connected to the side of the fixed disk (31) near the support plate. The liquid addition assembly (7) includes a lifting cylinder (71) and an enzyme solution storage tank (75) fixedly connected to the top cover (22). A first liquid addition rack (72) and a second liquid addition rack (73) are fixedly connected to the output end of the lifting cylinder (71). The first liquid addition rack (72) and the second liquid addition rack (73) are located directly above the drive assembly (4) and the centrifugation assembly (6), respectively. A liquid addition tube (74) is fixedly connected to the bottom of the first liquid addition rack (72) and the second liquid addition rack (73). The first liquid addition rack (72) and the second liquid addition rack (73) are respectively connected to the adjacent liquid addition tube (74). A liquid delivery tube (76) is also connected to the second liquid addition rack (73). The other end of the liquid delivery tube (76) is connected to the enzyme solution storage tank (75). The cutting assembly (3) also includes a cutting table (35) fixedly connected to the top cover (22). The cutting table (35) is located directly below the cutting blade (34), and the cutting table (35) has a cutting groove adapted to the cutting blade (34).

2. The umbilical cord mesenchymal stem cell separation device according to claim 1, characterized in that: The processing component (2) includes a processing box (21), a top cover (22) is fixedly connected to the top of the processing box (21), the cutting component (3) is set on the top of the top cover (22), the top of the top cover (22) has multiple sets of evenly distributed connecting grooves (23), a switching disk (24) is rotatably connected between the processing box (21) and the top cover (22), a switching disk (24) has multiple sets of rotating grooves (25), a processing cylinder (26) is rotatably connected in each set of rotating grooves (25), a centrifuge tube (27) is slidably inserted in each set of processing cylinders (26), an electric heating element for heating the solution in the centrifuge tube (27) is fixedly connected in each set of processing cylinders (26), a tube cap (28) is snapped on each set of centrifuge tubes (27), a one-way valve plate is hinged in the tube cap (28), and a connecting hole is opened at the bottom of each set of processing cylinders (26). The bottom of the switching disk (24) is also provided with a rotary motor (29), and the output end of the rotary motor (29) is fixed to the bottom of the switching disk (24).

3. The umbilical cord mesenchymal stem cell separation device according to claim 2, characterized in that: The fixed plate (31) is also fixedly connected to a first drive rod (32) on the side away from the support plate. A first drive frame (33) is sleeved on the first drive rod (32). A cutting blade (34) is fixedly connected to the bottom of the first drive frame (33). Guide frames (37) are also fixedly connected to both sides of the bottom of the first drive frame (33). The bottoms of the two sets of guide frames (37) penetrate the top cover (22) and extend into the processing box (21). The bottom of the cutting table (35) is connected to a guide box (36), and the other end of the guide box (36) extends into the processing box (21) and is connected to the adjacent centrifuge tube (27) in the processing box (21). The discharge end of the guide box (36) is also equipped with a metering valve.

4. The umbilical cord mesenchymal stem cell separation device according to claim 3, characterized in that: The drive assembly (4) includes a drive motor (41) fixedly connected to the processing box (21). The drive motor (41) is located in the processing box (21) on the side near the oscillation assembly (5). The output end of the drive motor (41) is fixedly connected to a first bevel gear (42). The side of the first bevel gear (42) near the drive motor (41) is fixedly connected to a second synchronous shaft. A synchronous belt (44) is sleeved on the second synchronous shaft. The other end of the synchronous belt (44) passes through the outside of the processing box (21) and is sleeved on the first synchronous shaft on the fixed plate (31). The second synchronous shaft is connected to the first synchronous shaft through the synchronous belt (44). The drive assembly (4) further includes a second bevel gear (43), which meshes with a first bevel gear (42). A synchronizing rod (45) is fixedly connected to the side of the first bevel gear (42) near the second bevel gear (43), and the synchronizing rod (45) is fixed to the conveying assembly (8).

5. The umbilical cord mesenchymal stem cell separation device according to claim 4, characterized in that: The oscillation assembly (5) includes a fixed frame (51) fixedly connected to the processing box (21), a second bevel gear (43) rotatably connected to the fixed frame (51), a transmission rod (52) rotatably connected to the fixed frame (51), and the transmission rod (52) passes through the fixed frame (51) on the side near the fixed frame (51) and is fixed to the second bevel gear (43). The other end of the transmission rod (52) is fixedly connected to a first transmission plate (53), and a push rod is fixedly connected to the side of the first transmission plate (53) away from the transmission rod (52).

6. The umbilical cord mesenchymal stem cell separation device according to claim 5, characterized in that: The oscillation assembly (5) further includes a rotating rod (54) rotatably connected to the fixed frame (51), and the rotating rod (54) is located directly below one of the connecting slots (23). The rotating rod (54) is located on the side of the fixed frame (51) away from the second bevel gear (43). Two sets of connecting frames (55) are fixedly connected to the rotating rod (54). A drive plate (56) is also hinged between the two sets of connecting frames (55). The drive plate (56) is located on the end of the connecting frame (55) away from the rotating rod (54). A sliding groove (57) is also provided on the side of the drive plate (56) away from the rotating rod (54). The end of the push rod on the first transmission plate (53) near the sliding groove (57) is slidably connected to the sliding groove (57). The top of the rotating rod (54) is also fixedly connected to a synchronous disk, and the synchronous disk is rotatably connected to the top of the fixed frame (51). The top of the synchronous disk is fixedly connected to a first electric push rod (58), and the output end of the first electric push rod (58) is fixedly connected to multiple sets of positioning rods (59) that are adapted to the connection hole.

7. The umbilical cord mesenchymal stem cell separation device according to claim 2, characterized in that: The centrifugal assembly (6) is located directly below one of the connecting slots (23). The centrifugal assembly (6) includes two sets of second electric push rods (61). Both sets of second electric push rods (61) are fixedly connected to the processing box (21). The output ends of the two sets of second electric push rods (61) are fixedly connected to a connecting plate (62). The top of the connecting plate (62) is rotatably connected to a centrifugal cylinder (63). A connecting rod that matches the bottom connecting hole of the processing cylinder (26) is fixedly connected to the inner wall of the bottom of the centrifugal cylinder (63). A centrifugal motor (64) is also fixedly connected to the bottom of the connecting plate (62). The output end of the centrifugal motor (64) passes through the connecting plate (62) and is fixed to the centrifugal cylinder (63).

8. The umbilical cord mesenchymal stem cell separation device according to claim 2, characterized in that: The first liquid feeding rack (72) is also connected to a connecting pipe (77), the other end of which extends into the processing box (21) and is connected to the conveying assembly (8).

9. The umbilical cord mesenchymal stem cell separation device according to claim 8, characterized in that: The conveying assembly (8) includes a second transmission plate (81) and a conveying piston (85). One end of the second transmission plate (81) is fixed to a synchronizing rod (45), and the other end of the second transmission plate (81) is fixedly connected to a second driving rod (82). A second driving frame (83) is sleeved on the second driving rod (82). A driving disk (84) is fixedly connected to the side of the second driving frame (83) near the second transmission plate (81). The conveying piston (85) is fixedly connected to the processing box (21). A piston rod is slidably connected in the conveying piston (85). The end of the driving disk (84) away from the second driving frame (83) is fixed to the top of the piston rod in the conveying piston (85). A guide rod is also fixedly connected to the end of the second driving frame (83) near the inner wall of the processing box (21). A guide groove for the guide rod to slide is opened on the inner wall of the processing box (21). The feed end of the conveying piston (85) is also connected to a conveying pipe (86). The other end of the conveying pipe (86) passes through the processing box (21) and extends to the outside of the processing box (21). The extended end of the conveying pipe (86) is connected to a centrifugal liquid storage box (13). The discharge end of the conveying pipe (86) is connected to a connecting pipe (77). Both the feed end and the discharge end of the conveying pipe (86) are equipped with a one-way valve.

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