Method for planting traditional Chinese medicinal material roughhaired holly based on earthworm compound fertilizer

Abstract

The invention discloses a planting method of traditional Chinese medicinal material roughhaired holly based on earthworm compound fertilizer, and relates to the technical field of roughhaired holly planting. According to the traditional Chinese medicinal material holly root planting method based on the pheretima compound fertilizer, double-layer porous pottery clay balls are filled with functional materials, and nutrient islands are prepared; healthy pheretima is selected for inoculation preparation, and the obtained nutrient island is buried in soil in advance; performing field planting of roughhaired holly and directional inoculation of pheretima; the position of the nutrient island is moved regularly, and after moving regulation is achieved, planting management of the roughhaired holly is conducted. By means of chemotaxis and photoperiod reaction of pheretima to humus and dynamic management of the movable nutrient island, nutrients are guided to be directionally released to the rhizosphere area, nutrient loss is reduced, the resource utilization efficiency is improved, and the problem of uneven nutrient distribution caused by traditional fertilization is avoided.

Description

Technical Field

[0001] This invention relates to the field of Ilex crenata cultivation technology, specifically to a method for cultivating Ilex crenata, a traditional Chinese medicinal herb, based on a compound fertilizer containing ground worm. Background Technology

[0002] As a common medicinal plant in southern my country, *Ilex asprella* has high medicinal value in its roots and leaves, and is widely used in clearing heat and detoxifying, and promoting body fluid production and quenching thirst. In recent years, with the increasing demand for standardized cultivation of Chinese medicinal materials, the scale of artificial cultivation of *Ilex asprella* has gradually expanded, but many technical challenges still exist in the cultivation process.

[0003] Traditional Ilex cornuta cultivation often employs extensive management methods, relying heavily on chemical fertilizers for soil fertility. Long-term, singular use of chemical fertilizers can easily lead to soil compaction, decreased organic matter, and disruption of the soil's micro-ecological balance. This not only affects root development and the accumulation of effective components but may also create risks of heavy metal and pesticide residues, reducing the quality of the medicinal material. Furthermore, as a deep-rooted plant, Ilex cornuta requires high soil aeration; insufficient soil aeration in traditional cultivation methods leads to decreased root vitality and slow growth.

[0004] In existing ecological planting techniques, although there have been attempts to use ground worms to improve the soil, the ground worms are scattered and the improvement area is uneven, making it difficult to accurately match the nutrient needs of the root system of *Ilex crenata*. Moreover, the synergistic effect between ground worms and soil microorganisms has not been fully utilized, resulting in low organic fertilizer conversion efficiency and poor nutrient supply timeliness, which leads to the aggravation of soil degradation in the planting area and restricts the development of the *Ilex crenata* industry. Summary of the Invention

[0005] To address the shortcomings of existing technologies, this invention provides a method for cultivating the medicinal herb *Ilex chinensis* based on a compound fertilizer containing *Gnaphalium affine*, thus solving the problems mentioned in the background section.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a method for cultivating the medicinal herb *Ilex chinensis* based on *Gastrodia elata* compound fertilizer, the method comprising the following specific steps:

[0007] Nutrient islands that need to be placed in planting soil are prepared by filling double-layer porous clay balls with functional materials.

[0008] The planting soil was pretreated and improved, and healthy ground worms were selected for inoculation. The resulting nutrient islands were buried in the soil in advance to complete the pre-activation.

[0009] After selecting and treating the seedlings of *Ilex crenata*, the *Ilex crenata* were planted and *Euphorbia milii* was inoculated in a directional manner.

[0010] After periodically moving the nutrient islands to achieve mobile regulation, cultivation and management of *Ilex crenata* can be carried out.

[0011] A further improvement of the technical solution of the present invention is that: the double-layer porous clay ball filled with functional materials is prepared into a nutrient island that needs to be placed in the planting soil, and the functional materials include a nutrient layer, an information layer and a microbial layer;

[0012] The nutrient layer includes: humic acid, earthworm manure, activated carbon, and decomposed branches of *Ilex chinensis* mixed in a mass ratio of 4:3:2:1, providing a continuous carbon source, with the humic acid purity ≥85%.

[0013] The information layer includes: 0.1% L-arginine and 5% root exudate extract of Ilex crenata, wherein the 0.1% L-arginine serves as a pheromone of Ilex crenata and the 5% root exudate extract of Ilex crenata contains ferulic acid and vanillic acid, which enhance chemotactic attraction.

[0014] The microbial layer comprises: a density of 1×10 8 CFU / g of Sphingosine monocytogenes and Chlorella vulgaris form a metabolic synergy with Pheretima aspergillum.

[0015] A further improvement of the technical solution of the present invention is that: the double-layer porous clay ball filled with functional material is prepared to be placed in the nutrient island of the planting soil. The double-layer porous clay ball has a diameter of 30cm, a wall thickness of 5cm, an outer layer porosity of 30% to 35% and an outer layer pore diameter controlled at 2 to 3mm for the entry and exit of the soil, and an inner layer porosity of 50% and an inner layer pore diameter of 0.5 to 1mm for loading functional material.

[0016] A further improvement to the technical solution of this invention lies in: the pretreatment and improvement of the planting soil, the selection of healthy earthworms for inoculation, and the pre-burying of the resulting nutrient islands into the soil to complete pre-activation, including:

[0017] Turn over the soil to a depth of 30cm, remove weeds, apply 200kg of decomposed rice husks and 50kg of quicklime per acre, adjust the soil pH to 6.5-7.0, remove stones, and lay drip irrigation network;

[0018] Also includes:

[0019] Select healthy ground worms and use 20 worms / m 2 The density was prepared for use, and the animals were fed with 5% extract of Ilex chinensis leaves for 3 days.

[0020] A further improvement to the technical solution of this invention is that: the pretreatment and improvement of the planting soil, the selection of healthy earthworms for inoculation, and the pre-burying of the resulting nutrient islands into the soil to complete pre-activation, further includes:

[0021] The nutrient islands should be buried in the soil 3 to 7 days before the planting of the Gooseberry, and arranged in a ring around the four sides of each Gooseberry planting hole with a spacing of 30cm. This ensures that the nutrient release covers the root radiation range, while avoiding excessive concentration of nutrients that could burn the roots. When burying, care should be taken to keep the nutrient islands intact.

[0022] Before burying the nutrient islands, spray 500 ml of 5% glucose solution onto the resulting nutrient islands to promote microbial proliferation and pheromone release.

[0023] A further improvement to the technical solution of this invention lies in: after selecting and treating the seedlings of *Ilex crenata*, the transplanting of *Ilex crenata* and the directional inoculation of *Euphorbia milii* include:

[0024] Select one-year-old seedlings of *Ilex chinensis* with a height ≥30cm, a ground diameter ≥0.5cm, and free from pests and diseases. Soak the roots in a rooting agent solution for 30 minutes. Dig planting holes at a spacing of 1.2m × 1.5m. Lay a 10cm layer of well-rotted straw at the bottom, and fill the middle layer with garden soil and cow manure in a 3:1 mass ratio. The rooting agent is a compound product containing root-promoting components such as indolebutyric acid and naphthaleneacetic acid, which can effectively stimulate root cell division, enhance root vitality, and accelerate the germination of new roots. During soaking, ensure that the roots are completely submerged to allow for full absorption of the agent, laying the foundation for rapid seedling establishment and growth after transplanting.

[0025] Also includes:

[0026] Place the *Ilex crenata* seedling in the center of the planting hole, backfill the soil to the root collar, compact it, and then water with a solution of 0.2% humic acid. The planting hole should be located in the center of the nutrient islands on all four sides. Specifically, planting is best done on a cloudy day or in the evening when the light is weak and evaporation is low, which helps reduce the risk of seedling transpiration and water loss. Before planting, apply a mixture of well-rotted organic fertilizer and superphosphate as base fertilizer to the bottom of the planting hole and mix it with the subsoil. Place the *Ilex crenata* seedling with its root ball in the center of the hole, ensuring that the roots are spread out and not curled up. Then backfill with loose and breathable topsoil to the root collar, pressing it lightly in layers to ensure close contact between the roots and the soil and avoid creating air pockets. Immediately after planting, water thoroughly with a solution of 0.2% humic acid added to the water. This will promote the healing of root wounds and improve the soil's water and fertilizer retention capacity. The watering should be thorough until water seeps out from the bottom of the planting hole to ensure that the roots fully absorb the water.

[0027] Also includes:

[0028] On the third day after transplanting, dig a 5cm deep circular trench on all four sides of the nutrient island, put in the fed earthworms, cover with moist straw and water to keep it moist. This operation can accelerate the decomposition and transformation of straw by taking advantage of the earthworms' hygroscopic and fertile properties. At the same time, the vermicomposting tunnels generated by the earthworms' activity can significantly improve the permeability of the rhizosphere soil.

[0029] A further improvement to the technical solution of this invention lies in: the periodic relocation of the nutrient islands to achieve movement regulation, followed by cultivation management of *Ilex crenata*, includes:

[0030] Move the nutrient island every 30 days, shifting it 50cm horizontally along the planting row direction to ensure uniform soil improvement in the root zone. The movement should be done on a cloudy morning. After moving, supplement with Sphingosine Bacillus, Chlorella vulgaris, and 0.1% L-arginine and 5% Ilex cornuta root exudate extract to maintain attractiveness.

[0031] Also includes:

[0032] When the seedlings of *Ilex chinensis* reach a height of 50cm, pinch off the top, retaining 3-4 main branches, and spray with 0.3% potassium dihydrogen phosphate and 0.2% amino acids monthly;

[0033] Weeding and cultivating should be done quarterly to avoid damaging the activity channels of the earthworms. Phosphorus and potassium fertilizers should be applied in April and May each year and released through the nutrient island replenishment bins to promote flowering and fruiting.

[0034] Turn on drip irrigation when soil moisture is below 50% to keep the root zone moist but not waterlogged.

[0035] A further improvement to the technical solution of the present invention is that the method further includes:

[0036] At least 10% of the field ridge area is reserved as nutrient islands to serve as wintering habitats for ground worms. A 5cm thick layer of straw is laid for insulation. During the growth of the seedlings of *Ilex chinensis*, cow manure is spread daily.

[0037] Beneficial effects

[0038] Compared with the prior art, the beneficial effects of the present invention are: by utilizing the chemotaxis and photoperiodic response of the earthworm to humus, combined with the dynamic management of the movable nutrient island, nutrients are guided to be released in the rhizosphere region, reducing nutrient loss, improving resource utilization efficiency, and avoiding the problem of uneven nutrient distribution caused by traditional fertilization.

[0039] Using decomposed branches of *Ilex crenata* and agricultural waste such as *Eriocaulon buergerianum* manure as nutrient sources reduces the input of chemical fertilizers and lowers the risk of environmental pollution during the planting process. This aligns with the concept of green planting of Chinese medicinal herbs. Nutrients are provided through biological transformation, avoiding chemical fertilizer residues. At the same time, the natural active substances in the metabolites of *Eriocaulon buergerianum* can promote the secondary metabolism of *Ilex crenata* and enhance the accumulation of medicinal components. Attached Figure Description

[0040] Figure 1 This is a schematic diagram of the process of the present invention. Detailed Implementation

[0041] Various exemplary embodiments, features, and aspects of this application will now be described in detail with reference to the accompanying drawings. The same reference numerals in the drawings denote elements that have the same or similar functions. Although various aspects of the embodiments are shown in the drawings, they are not necessarily drawn to scale unless specifically indicated otherwise.

[0042] The term “exemplary” as used herein means “serving as an example, embodiment, or illustration.” Any embodiment illustrated herein as “exemplary” is not necessarily to be construed as superior to or better than other embodiments.

[0043] Furthermore, to better illustrate this application, numerous specific details are provided in the following detailed embodiments. Those skilled in the art should understand that this application can be implemented without certain specific details. In some instances, methods, means, and elements well-known to those skilled in the art have not been described in detail in order to highlight the main points of this application.

[0044] This invention provides a method for cultivating the medicinal herb *Ilex chinensis* based on a compound fertilizer containing *Gnaphalium affine*. The method includes the following specific steps:

[0045] Nutrient islands that need to be placed in planting soil are prepared by filling double-layer porous clay balls with functional materials.

[0046] The planting soil was pretreated and improved, and healthy ground worms were selected for inoculation. The resulting nutrient islands were buried in the soil in advance to complete the pre-activation.

[0047] After selecting and treating the seedlings of *Ilex crenata*, the *Ilex crenata* were planted and *Euphorbia milii* was inoculated in a directional manner.

[0048] After periodically moving the nutrient islands to achieve mobile regulation, cultivation and management of *Ilex crenata* can be carried out.

[0049] Based on functional materials filled with double-layer porous clay balls, nutrient islands that need to be placed in planting soil are prepared. The functional materials include a nutrient layer, an information layer, and a microbial layer.

[0050] The nutrient layer includes: humic acid, earthworm manure, activated carbon, and decomposed branches of *Ilex chinensis* mixed in a mass ratio of 4:3:2:1, providing a continuous carbon source, with humic acid purity ≥85%.

[0051] The information layer includes: 0.1% L-arginine and 5% root secretion extract of Ilex crenata. The 0.1% L-arginine serves as a pheromone of Ilex crenata, and the 5% root secretion extract of Ilex crenata contains ferulic acid and vanillic acid, which enhance chemotactic attraction.

[0052] The microbial layer consists of a density of 1×10⁻⁶. 8 CFU / g of Sphingosine monocytogenes and Chlorella vulgaris form a metabolic synergy with Pheretima aspergillum.

[0053] Based on functional materials, double-layer porous clay balls are prepared to be placed in the nutrient islands of planting soil. The double-layer porous clay balls have a diameter of 30cm and a wall thickness of 5cm. The outer layer has a porosity of 30% to 35% and an outer pore diameter of 2 to 3mm to allow the earthworm to enter and exit. The inner layer has a porosity of 50% and an inner pore diameter of 0.5 to 1mm to load the functional materials.

[0054] The planting soil was pretreated and improved, and healthy earthworms were selected for inoculation. The resulting nutrient islands were buried in the soil in advance to complete the pre-activation process, including:

[0055] Turn over the soil to a depth of 30cm, remove weeds, apply 200kg of decomposed rice husks and 50kg of quicklime per acre, adjust the soil pH to 6.5-7.0, remove stones, and lay drip irrigation network;

[0056] Also includes:

[0057] Select healthy ground worms and use 20 worms / m 2 The density was prepared for use, and the animals were fed with 5% extract of Ilex chinensis leaves for 3 days.

[0058] The planting soil is pre-treated and improved, and healthy earthworms are selected for inoculation. The resulting nutrient islands are buried in the soil in advance to complete the pre-activation process. This also includes:

[0059] The nutrient islands should be buried in the soil 3 to 7 days before the planting of the Gooseberry, and arranged in a ring around the four sides of each Gooseberry planting hole with a spacing of 30cm. This ensures that the nutrient release covers the root radiation range, while avoiding excessive concentration of nutrients that could burn the roots. When burying, care should be taken to keep the nutrient islands intact.

[0060] Before burying the nutrient islands, spray 500 ml of 5% glucose solution onto the resulting nutrient islands to promote microbial proliferation and pheromone release.

[0061] After selecting and treating the seedlings of *Ilex crenata*, the *Ilex crenata* were transplanted and *Pheretima aspergillum* was directionally inoculated, including:

[0062] Select one-year-old seedlings of *Ilex chinensis* with a height ≥30cm, a ground diameter ≥0.5cm, and free from pests and diseases. Soak the roots in a rooting agent solution for 30 minutes. Dig planting holes at a spacing of 1.2m × 1.5m. Lay a 10cm layer of well-rotted straw at the bottom, and fill the middle layer with garden soil and cow manure in a 3:1 mass ratio. The rooting agent is a compound product containing root-promoting components such as indolebutyric acid and naphthaleneacetic acid, which can effectively stimulate root cell division, enhance root vitality, and accelerate the germination of new roots. During soaking, ensure that the roots are completely submerged to allow for full absorption of the agent, laying the foundation for rapid seedling establishment and growth after transplanting.

[0063] Also includes:

[0064] Place the *Ilex crenata* seedling in the center of the planting hole, backfill the soil to the root collar, compact it, and then water with a solution of 0.2% humic acid. The planting hole should be located in the center of the nutrient islands on all four sides. Specifically, planting is best done on a cloudy day or in the evening when the light is weak and evaporation is low, which helps reduce the risk of seedling transpiration and water loss. Before planting, apply a mixture of well-rotted organic fertilizer and superphosphate as base fertilizer to the bottom of the planting hole and mix it with the subsoil. Place the *Ilex crenata* seedling with its root ball in the center of the hole, ensuring that the roots are spread out and not curled up. Then backfill with loose and breathable topsoil to the root collar, pressing it lightly in layers to ensure close contact between the roots and the soil and avoid creating air pockets. Immediately after planting, water thoroughly with a solution of 0.2% humic acid added to the water. This will promote the healing of root wounds and improve the soil's water and fertilizer retention capacity. The watering should be thorough until water seeps out from the bottom of the planting hole to ensure that the roots fully absorb the water.

[0065] Also includes:

[0066] On the third day after transplanting, dig a 5cm deep circular trench on all four sides of the nutrient island, put in the fed earthworms, cover with moist straw and water to keep it moist. This operation can accelerate the decomposition and transformation of straw by taking advantage of the earthworms' hygroscopic and fertile properties. At the same time, the vermicomposting tunnels generated by the earthworms' activity can significantly improve the permeability of the rhizosphere soil.

[0067] After periodically relocating the nutrient islands to achieve mobile regulation, cultivation and management of *Ilex crenata* are carried out, including:

[0068] Move the nutrient island every 30 days, shifting it 50cm horizontally along the planting row direction to ensure uniform soil improvement in the root zone. The movement should be done on a cloudy morning. After moving, supplement with Sphingosine Bacillus, Chlorella vulgaris, and 0.1% L-arginine and 5% Ilex cornuta root exudate extract to maintain attractiveness.

[0069] Also includes:

[0070] When the seedlings of *Ilex chinensis* reach a height of 50cm, pinch off the top, retaining 3-4 main branches, and spray with 0.3% potassium dihydrogen phosphate and 0.2% amino acids monthly;

[0071] Weeding and cultivating should be done quarterly to avoid damaging the activity channels of the earthworms. Phosphorus and potassium fertilizers should be applied in April and May each year and released through the nutrient island replenishment bins to promote flowering and fruiting.

[0072] Turn on drip irrigation when soil moisture is below 50% to keep the root zone moist but not waterlogged.

[0073] The method also includes:

[0074] At least 10% of the field ridge area is reserved as nutrient islands to serve as wintering habitats for ground worms. A 5cm thick layer of straw is laid for insulation. During the growth of the seedlings of *Ilex chinensis*, cow manure is spread daily.

[0075] In some embodiments, six solar-powered infrared lamps are installed per acre, spaced 15 meters apart, to form a gradient light field in conjunction with the light source on the nutrient islands, inducing the soil dragons to gather towards the root zone. Sensors are buried 50 cm around the nutrient islands to monitor soil temperature, humidity, and electrical conductivity in real time, and the data is transmitted wirelessly to a control terminal. Furthermore, a replaceable nutrient supplementation chamber is designed, containing slow-release nitrogen, phosphorus, and potassium composite granules with N:P2O5:K2O = 3:1:2, which are automatically released when the available nitrogen in the soil falls below 60 mg / kg.

[0076] In some embodiments, the prepared nutrient island is equipped with a detachable nylon strap at the bottom and an infrared light emitting module at the top, and is automatically turned on at night by a timer controller.

[0077] In summary, because the chemotaxis of *Echinochloa crus-galli* towards humic substances (i.e., recognizing humic acid molecule signals through olfactory receptors) and its photoperiodic response (i.e., its diurnal activity rhythm of being attracted to infrared light at night and avoiding light during the day) are characteristics of *Echinochloa crus-galli*, the nutrient islands prepared in the above-mentioned process only release the attraction signals of *Echinochloa crus-galli*, i.e., the chemical characteristics, while the physical characteristics of light environment regulation can be achieved synergistically. The light source should be turned off for two weeks before the directional inoculation of *Echinochloa crus-galli* to allow it to adapt to the environment. Photoperiodic regulation should be turned on from the 15th day, with the lights turned on from 18:00 to 6:00 at night.

[0078] In some embodiments, to verify the outstanding effects of the technical solution of this application, multiple sets of control experiments are provided, as shown below:

[0079] Control group 1 – blank control group, the planting method was as follows:

[0080] Only 30cm deep was tilled, weeds and stones were removed, no soil amendments were applied, and the pH was left to its natural state.

[0081] Plant at a spacing of 1.2m x 1.5m, filling the planting holes with only garden soil, without applying straw or manure.

[0082] No earthworms were inoculated throughout the entire process, and no nutrient islands or tropism regulation devices were set up.

[0083] Fertilizer management: No fertilizer is applied; only drip irrigation with clean water is used during dry periods.

[0084] Field management: Routine weeding and cultivation, without special adjustments.

[0085] The control group consisted of two subgroups: the traditional fertilizer planting group and the control group. The traditional fertilizer planting method was the same as the experimental group, with deep plowing and pH adjustment (6.5-7.0), and no application of decomposed rice husks. Before transplanting, 50 kg of compound fertilizer per mu was applied, evenly spread, and then rotary tilled into the soil. During the seedling stage, 0.3% potassium dihydrogen phosphate was applied, and during the mature stage, 15 kg of urea + 10 kg of potassium chloride per mu was applied by trenching and burying. The seedling selection and transplanting method were the same as the experimental group. The planting holes were not filled with straw, but backfilled with garden soil. No ground sluice gates were used, and no nutrient islands or light control equipment were set up. Drip irrigation based on soil moisture sensors was used, just like the experimental group.

[0086] The control group consisted of three groups: the fixed nutrient island group and the planting method was the same as that of the experimental group. The same nutrient islands and filling materials were used as in the experimental group, but the plants were fixed in their initial positions after planting. The inoculation of *Eriocaulon buergerianum* was carried out at the same density and timing as in the experimental group. Infrared light and pheromone supplementation were turned on, but the position of the nutrient islands remained unchanged. The supplementation of functional microbial agents and water management were the same as in the experimental group.

[0087] In summary, the experimental design parameters specifically include:

[0088] The planting site should be red soil with an initial organic matter content of 18-20 g / kg. The planting density should be 1.2 m × 1.5 m between plants and rows, with 3 replicates. The planting should be arranged in randomized blocks and a 50 cm wide isolation strip should be set up to prevent the ground dragonfly from moving across the area.

[0089] In terms of testing indicators, the effectiveness of soil improvement includes soil physicochemical indicators—organic matter content, available nitrogen, available phosphorus, available potassium, soil bulk density, and soil biological indicators—urease activity, phosphatase activity, and microbial diversity.

[0090] The growth indicators of Ilex crenata include plant height, ground diameter, number of branches, fresh weight per plant, taproot length, number of lateral roots ≥5cm, root-to-shoot ratio, chlorophyll content, and root activity.

[0091] The activity of *Earthworm* includes its density, distribution uniformity, and manure production.

[0092] The quality of medicinal materials includes the content of gallic acid and quercetin in the roots, the content of lead, cadmium and arsenic in the roots, the yield of fresh roots per plant and the total yield.

[0093] The results are shown in the table below:

[0094] Table 1

[0095]

[0096] In summary, this invention, based on the behavioral ecology of *Gnaphalium affine*, utilizes the chemotaxis and photoperiodic response of *Gnaphalium affine* to humus to release attraction signals and guides the formation of dynamic improvements in the rhizosphere through light environment regulation, thus solving the problems of dispersed activity and uneven rhizosphere improvement in traditional *Gnaphalium affine* farming.

[0097] Furthermore, as a typical deep-rooted Chinese medicinal herb, *Ilex chinensis* requires a loose, well-aerated, and organic-rich soil environment for root development. The directional movement of earthworms can increase the porosity of the rhizosphere soil. Simultaneously, the humic acid in the earthworm's metabolites can promote the accumulation of secondary metabolites in *Ilex chinensis*, improving the quality of the medicinal material. The regularly applied cow manure not only serves as food for the earthworms but also provides fertilizer for the *Ilex chinensis*.

[0098] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0099] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A method for cultivating the medicinal herb *Ilex chinensis* based on a compound fertilizer containing *Gnaphalium affine*, characterized in that... The method includes the following specific steps: Nutrient islands that need to be placed in planting soil are prepared by filling double-layer porous clay balls with functional materials. The planting soil was pretreated and improved, and healthy ground worms were selected for inoculation. The resulting nutrient islands were buried in the soil in advance to complete the pre-activation. After selecting and treating the seedlings of *Ilex crenata*, the *Ilex crenata* were planted and *Euphorbia milii* was inoculated in a directional manner. After periodically moving the nutrient islands to achieve mobile regulation, cultivation and management of *Ilex crenata* can be carried out.

2. The method for cultivating the medicinal herb *Ilex chinensis* based on *Gnaphalium affine* compound fertilizer according to claim 1, characterized in that... The method involves filling a double-layer porous clay ball with functional materials to create a nutrient island that needs to be placed in the planting soil. The functional materials include a nutrient layer, an information layer, and a microbial layer. The nutrient layer comprises: humic acid, earthworm manure, activated carbon, and decomposed branches of *Ilex chinensis* mixed in a mass ratio of 4:3:2:1, with the humic acid purity ≥85%. The information layer includes: 0.1% L-arginine and 5% extract of root exudate from *Ilex crenata*. The microbial layer comprises: a density of 1×10 8 CFU / g of Sphingosine monocytogenes and Chlorella vulgaris.

3. The method for cultivating the medicinal herb *Ilex chinensis* based on *Gnaphalium affine* compound fertilizer according to claim 1, characterized in that... The double-layer porous clay ball, filled with functional materials, is prepared to be placed in the nutrient island of the planting soil. The double-layer porous clay ball has a diameter of 30cm, a wall thickness of 5cm, an outer layer porosity of 30% to 35% and an outer layer pore diameter controlled at 2 to 3mm, an inner layer porosity of 50% and an inner layer pore diameter of 0.5 to 1mm.

4. The method for cultivating the medicinal herb *Ilex chinensis* based on *Pheretima aspergillum* compound fertilizer according to claim 1, characterized in that... The process of pre-treating and improving the planting soil, selecting healthy earthworms for inoculation, and pre-burying the resulting nutrient islands in the soil to complete pre-activation includes: Turn over the soil to a depth of 30cm, remove weeds, apply 200kg of decomposed rice husks and 50kg of quicklime per acre, adjust the soil pH to 6.5-7.0, remove stones, and lay drip irrigation network; Also includes: Select healthy ground worms and use 20 worms / m 2 The density was prepared for use, and the animals were fed with 5% extract of Ilex chinensis leaves for 3 days.

5. The method for cultivating the medicinal herb *Ilex chinensis* based on *Gnaphalium affine* compound fertilizer according to claim 4, characterized in that... The process of pre-treating and improving the planting soil, selecting healthy earthworms for inoculation, and burying the resulting nutrient islands in the soil in advance to complete pre-activation also includes: The obtained nutrient islands were buried in the soil 3 to 7 days before the planting of the Gooseberry, and arranged in a ring around the four sides of each Gooseberry planting hole with a spacing of 30cm. Before burying it in the soil, spray 500ml of 5% glucose solution onto the resulting nutrient islands.

6. The method for cultivating the medicinal herb *Ilex chinensis* based on *Gnaphalium affine* compound fertilizer according to claim 1, characterized in that... After selecting and treating the seedlings of *Ilex crenata*, the transplanting of *Ilex crenata* and the targeted inoculation of *Pheretima aspergillum* include: Select one-year-old seedlings of *Ilex chinensis* with a plant height ≥30cm, a ground diameter ≥0.5cm, and free from diseases and pests. Soak the roots in a rooting agent solution for 30 minutes. Dig planting holes at a spacing of 1.2m×1.5m. Lay a 10cm thick layer of decomposed straw at the bottom and fill the middle layer with garden soil and cow manure in a mass ratio of 3:

1. Also includes: Place the seedling of *Ilex chinensis* in the center of the planting hole, backfill the soil to the root collar, compact it, and then water it with water containing 0.2% humic acid. The planting hole is located in the center of the nutrient islands set on the four sides. Also includes: On the third day after transplanting, dig a 5cm deep circular trench on all four sides of the nutrient island, put in the fed earthworms, cover with moist straw and water to keep them moist.

7. The method for cultivating the medicinal herb *Ilex chinensis* based on *Gnaphalium affine* compound fertilizer according to claim 1, characterized in that... The periodic relocation of the nutrient islands, after achieving movement regulation, is followed by the cultivation and management of *Ilex crenata*, including: Move the nutrient island position every 30 days, shifting it 50cm horizontally along the planting row direction. After moving, supplement with Sphingosine Bacillus, Chlorella vulgaris, and 0.1% L-arginine and 5% Ilex cornuta root exudate extract. Also includes: When the seedlings of *Ilex chinensis* reach a height of 50cm, pinch off the top, retaining 3-4 main branches, and spray with 0.3% potassium dihydrogen phosphate and 0.2% amino acids monthly; Weeding and cultivating should be done every quarter, and phosphorus and potassium fertilizers should be applied in April and May each year. Turn on drip irrigation when soil moisture is below 50%.

8. The method for cultivating the medicinal herb *Ilex chinensis* based on *Gnaphalium affine* compound fertilizer according to claim 1, characterized in that... The method further includes: At least 10% of the field ridge area is reserved as nutrient islands to serve as wintering habitats for ground worms. A 5cm thick layer of straw is laid for insulation. During the growth of the seedlings of *Ilex chinensis*, cow manure is spread daily.

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