A staged feed additive for broilers, its usage and application

Abstract

This invention discloses a staged combined feed additive for broilers, its application method, and its application, belonging to the field of poultry farming technology. The invention discloses a staged combined feed additive for broilers, comprising a stage one additive and a stage two additive; the stage one additive includes a Bacillus amyloliquefaciens TL preparation, and the stage two additive includes a Lactobacillus gasseri preparation. This invention, through the combined application of these two strains, promotes the expression of genes related to lipid synthesis and regulates host lipid metabolism, significantly improving broiler growth performance and playing a vital role in enhancing broiler farming efficiency.

Description

Technical Field

[0001] This invention belongs to the field of poultry farming technology, and in particular relates to a staged feed additive for broilers, its usage method and application. Background Technology

[0002] In modern animal husbandry, probiotics, as a green alternative to antibiotics, are increasingly widely used in broiler farming. Bacillus amyloliquefaciens TL, a highly effective probiotic isolated from Tongcheng pigs, has been shown in previous studies to significantly promote the growth of white-feathered broilers in the early stages of broiler farming (1-21 days old). However, its growth-promoting effect slows significantly between 22-35 days of age, limiting its application throughout the entire growth cycle of broilers.

[0003] Currently, the application of single probiotics in animal husbandry has limitations, especially in its insufficient sustained promotion of broiler growth performance in the later stages of growth. Existing research also lacks clarity on how probiotics regulate the gut microbiota of broilers. Therefore, finding a rational combination of probiotics to compensate for the performance deficiencies of single strains has become a key issue in improving broiler farming efficiency. Summary of the Invention

[0004] In view of this, the present invention discloses a staged combined feed additive for broilers, its usage method and application.

[0005] The present invention adopts the following technical solution:

[0006] A phased feed additive for broilers, comprising a phase one additive and a phase two additive; the phase one additive comprises a Bacillus amyloliquefaciens TL preparation, and the phase two additive comprises a Lactobacillus gasseri preparation.

[0007] Furthermore, the viable count of the *Bacillus amyloliquefaciens* TL preparation is ≥1.0 × 10⁻⁶. 10 CFU / g.

[0008] Furthermore, the viable count of the Lactobacillus gasseri preparation is ≥1.0 × 10⁻⁶. 9 CFU / kg.

[0009] Furthermore, the amount of the Bacillus amyloliquefaciens TL preparation added to the feed is 200 mg / kg.

[0010] Furthermore, the amount of the Lactobacillus gasseri preparation added to the feed is 1000 mg / kg.

[0011] A method of using the above-mentioned feed additive, wherein the Bacillus amyloliquefaciens TL preparation is used as a feed additive for broiler chickens in the early stage; and the Lactobacillus gasseri preparation is used as a feed additive for broiler chickens in the later stage.

[0012] Furthermore, the early stage includes 1-21 days of age.

[0013] Furthermore, the later stage includes 22-35 days of age.

[0014] An application of the above-mentioned feed additive, wherein the additive is used to improve the glucose and lipid metabolism of broilers.

[0015] Furthermore, the glucose and lipid metabolism includes: significantly increasing the levels of very low density lipoprotein, triglycerides, free fatty acids and blood glucose in broiler serum, while upregulating insulin-like growth factor-1 expression; promoting lipid synthesis and transport, and accelerating gluconeogenesis.

[0016] The source of the probiotic preparation involved in the technical solution of this invention is explained as follows: Bacillus amyloliquefaciens TL was deposited at the China Center for Type Culture Collection (CCTCC) at Wuhan University in Wuhan, Hubei Province on June 4, 2015, with accession number CCTCC NO:M 2015359. The telephone number of the collection center is 027-68754052. Bacillus amyloliquefaciens TL can be obtained from the collection center or purchased from Hubei Huada Ruier Technology Co., Ltd. The Lactobacillus gasseri preparation is a conventional preparation and can be obtained through conventional purchase channels.

[0017] The beneficial effects of this invention are:

[0018] Improved Growth Performance: This invention discloses a staged combined feed additive for broilers. By feeding broilers with Bacillus amyloliquefaciens TL and Lactobacillus gasseri preparations at different stages of broiler rearing, with the addition of Bacillus amyloliquefaciens TL in the early stage and Lactobacillus gasseri in the later stage, the body weight, daily weight gain, and daily weight gain rate of broilers aged 22-35 days are significantly improved, thus significantly increasing broiler breeding efficiency. As shown in Example 2, compared with the control group fed a diet without the additive in the later stage, the daily weight gain increased by 33.3%, and the feed conversion ratio decreased by 22.1%.

[0019] Enhanced metabolic regulation: The phased combined use of two probiotics can regulate serum glucose and lipid metabolism indicators, significantly increasing the levels of very low-density lipoprotein (VLDL), triglycerides (TG), free fatty acids (FFA), and blood glucose (GLU) in broiler serum, while upregulating the expression of insulin-like growth factor-1 (IGF-1). This can promote lipid synthesis and transport, accelerate gluconeogenesis, and achieve precise regulation of energy metabolism, which is of great significance for improving broiler farming efficiency. Detailed Implementation

[0020] To better understand the technical solution of the present invention, the embodiments of the present invention will be described in detail below.

[0021] It should be understood that the described embodiments are merely some, not all, of the embodiments of the present invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without inventive effort are within the scope of protection of the present invention.

[0022] Example 1

[0023] A phased feed additive for broilers, comprising a phase one additive and a phase two additive; the phase one additive comprises a Bacillus amyloliquefaciens TL preparation, and the phase two additive comprises a Lactobacillus gasseri preparation.

[0024] Furthermore, the viable count of the *Bacillus amyloliquefaciens* TL preparation is ≥1.0 × 10⁻⁶. 10 CFU / g.

[0025] Furthermore, the viable count of the Lactobacillus gasseri preparation is ≥1.0 × 10⁻⁶. 9 CFU / kg.

[0026] Furthermore, the amount of the Bacillus amyloliquefaciens TL preparation added to the feed is 200 mg / kg.

[0027] Furthermore, the amount of the Lactobacillus gasseri preparation added to the feed is 1000 mg / kg.

[0028] A method of using the above-mentioned feed additive, wherein the Bacillus amyloliquefaciens TL preparation is used as a feed additive for broiler chickens in the early stage; and the Lactobacillus gasseri preparation is used as a feed additive for broiler chickens in the later stage.

[0029] Furthermore, the early stage includes 1-21 days of age.

[0030] Furthermore, the later stage includes 22-35 days of age.

[0031] An application of the above-mentioned feed additive, wherein the additive is used to improve the glucose and lipid metabolism of broilers.

[0032] Furthermore, the glucose and lipid metabolism includes: significantly increasing the levels of very low density lipoprotein, triglycerides, free fatty acids and blood glucose in broiler serum, while upregulating insulin-like growth factor-1 expression; promoting lipid synthesis and transport, and accelerating gluconeogenesis.

[0033] Example 2

[0034] 1. Effects of staged feeding on the growth performance of broiler chickens

[0035] Male white-feathered broiler chickens were selected for the experiment. There was no statistically significant difference in initial body weight among the chickens at one day of age. Each group had three replicates, with eight chickens per replicate. A standardized cage rearing system was used during the experiment, with a stocking density of 10-12 chickens per cage. Sufficient feed and water were ensured at all times. Daily feed intake was recorded, and individual body weight and health status were measured weekly. The basal diet was produced and processed by Chengxin Feed Store in Nanchang Economic and Technological Development Zone. The basal diet formula is shown in Table 1. The viable count of Bacillus amyloliquefaciens TL preparation was 1.0 × 10⁻⁶. 10 CFU / g; viable count of Lactobacillus gasseri, Pediococcus lactis, and Lactobacillus plantarum preparations was 1.0 × 10⁻⁶. 9 CFU / kg. Preparations of *Pediococcus lactis* and *Lactobacillus plantarum* are standard preparations and can be obtained through regular purchasing channels.

[0036] Table 1. Basal Diet Formula

[0037]

[0038] The grouping of the phased feeding experiment of broiler chickens is shown in Table 2. Table 3 shows the weekly weight records of broiler chickens from 1 to 21 days and Table 4 shows the growth performance of broiler chickens from 21 to 35 days.

[0039] Table 2 Feeding conditions of chickens in the experimental groups

[0040]

[0041] Table 3 Weekly body weight of broiler chickens (1-21 days) (unit: g)

[0042]

[0043] Table 4 Growth performance of broiler chickens from 21 to 35 days

[0044]

[0045] All four groups of broilers showed a typical weight gain trend during the baseline period (1-21 days), with no statistically significant differences among the groups, indicating that the broiler weight levels remained consistent during this period. From 21 to 35 days, the average weight gain of groups G, P, and Z was 33.33%, 29.14%, and 32.66% higher than that of group C, respectively; their feed conversion ratios decreased by 22.13%, 20.00%, and 17.45%, respectively. Therefore, blood parameters were measured in groups G and C.

[0046] 2. Effects of staged feeding on blood parameters

[0047] The ELISA kit measures glucose and lipid metabolism parameters including very low-density lipoprotein (VLDL), insulin (INS), growth hormone (GH), and insulin-like growth factor 1 (IGF-1). Follow the instructions provided with the corresponding kit; the instructions for the ordered kit shall prevail. Serum glucose (GLU), free fatty acids (FFA), and triglycerides (TG) will be measured using the kit.

[0048] Serum biochemical tests showed that the Lactobacillus gasseri intervention group (G group) achieved a systemic metabolic upgrade compared with the control group (C group): blood glucose (GLU-YA) level increased significantly by 36.0%, laying the foundation for the body's energy supply; free fatty acids (FFA) and triglycerides (TG) increased significantly by 57.1% and 31.4% respectively, while very low density lipoprotein (VLDL) showed an upward trend of 16.1%, confirming the dual enhancement of lipid mobilization and synthesis; insulin-like growth factor-1 (IGF-1) increased by 19.8%, directly driving growth and development.

[0049] The effects of *Lactobacillus gasseri* treatment on glucose and lipid metabolism and growth regulation in broilers were assessed by measuring serum biochemical and growth-related indicators. Regarding lipid metabolism, the second-stage feeding with *Lactobacillus gasseri* supplement significantly increased very low-density lipoprotein (VLDL) levels, suggesting that it may promote lipid metabolism by enhancing hepatic lipid synthesis or transport mechanisms. Furthermore, triglyceride (TG) levels in group G were significantly higher than in the control group, and their free fatty acid (FFA) concentrations were also significantly increased, indicating that *Lactobacillus gasseri* may affect energy metabolism by inhibiting lipolysis or enhancing lipid accumulation pathways. Regarding glucose metabolism, blood glucose (GLU-YA) levels in group G were significantly higher than in the control group, but there was no significant difference in insulin concentration between the two groups, suggesting that several probiotics may inhibit glycolysis and promote gluconeogenesis. Growth-related hormone analysis showed that insulin-like growth factor-1 (IGF-1) levels in group G were significantly higher than in the control group, while growth hormone (GH) levels did not differ significantly between the two groups, indicating that *Lactobacillus gasseri* may promote broiler growth by activating the IGF-1 synthesis pathway rather than directly regulating GH secretion.

[0050] Table 5 Serum biochemical indicators at 35 days of age

[0051]

[0052] Example 3

[0053] To investigate the effects of a feeding regimen of Bacillus amyloliquefaciens TL from 1-21 days of age and Lactobacillus gasseri from 22-35 days of age on the growth performance of broiler chickens, the following experiment was conducted on a farm.

[0054] 1. Experimental Materials and Methods

[0055] 1.1 Laboratory animals and feeding

[0056] The experiment was conducted at a breeding base in Hubei Province, using 9,000 male broiler chickens. There was no statistically significant difference in initial weight among the chickens at one day of age. Each group had three replicates, with 500 chickens per replicate. A standardized cage rearing system was used during the experiment, with a stocking density of 10-12 chickens per cage. Sufficient feed and water were ensured at all times. Daily feed intake was recorded, and individual weight and health status were measured weekly. The diet, Bacillus amyloliquefaciens TL, and Lactobacillus gasseri were the same as in Example 2.

[0057] Table 6 Feeding conditions of chickens in the experimental groups

[0058]

[0059] During the experiment, a standardized cage rearing model was adopted, with a stocking density of 10-12 individuals per cage. Sufficient feed and water were always provided. Daily feed intake was recorded, and individual weight and health status were measured weekly.

[0060] Average daily weight gain (ADG) = (Final weight - Initial weight) / Number of days in the trial

[0061] Average daily feed intake (AFDI) = Feed intake / (Number of experimental days × Number of experimental chickens)

[0062] Feed / Gain Rate (F / G) = Average daily feed intake / Average daily weight gain.

[0063] Table 7 Growth performance of 1-35 day old broiler chickens

[0064]

[0065] Table 7 shows that, compared with the control group, the experimental group of 1-5 day broiler chickens showed increased weight gain of 29.69%, 28.43%, 14.61%, 9.54%, and 15.70%, respectively; increased average daily feed intake of 9.94%, 9.88%, 6.34%, 4.77%, and 5.72%, respectively; and decreased average feed conversion ratio of 15.34%, 14.20%, 7.39%, 4.55%, and 8.52%, respectively. Adding Bacillus amyloliquefaciens TL during the 1-21 day period and Lactobacillus gasseri during the 22-35 day period showed better effects, and the effects of adding 500 mg / kg and 1000 mg / kg during the 22-35 day period were similar. This experiment demonstrates that adding Bacillus amyloliquefaciens TL in the early stage (1-21d) and Lactobacillus gasseri in the later stage (22-35d) has a better effect on improving production performance than adding Lactobacillus gasseri in the early stage and Bacillus amyloliquefaciens TL in the later stage, or adding a mixed formulation of Bacillus amyloliquefaciens TL and Lactobacillus gasseri simultaneously without different stages. This shows that the phased combined use of feed in this invention is more effective when used in stages and in sequence.

[0066] The embodiments of the present invention have been described in detail above. Specific examples have been used to illustrate the principles and implementation methods of the present invention. The description of the above embodiments is only for the purpose of helping to understand the method and core ideas of the present invention. At the same time, for those skilled in the art, there will be changes in the specific implementation methods and application scope based on the ideas of the present invention. Therefore, the content of this specification should not be construed as a limitation of the present invention.

Claims

1. A broiler chicken multi-stage feed additive, characterized in that, The additive comprises a phase one additive and a phase two additive; the phase one additive comprises a Bacillus amyloliquefaciens TL preparation, and the phase two additive comprises a Lactobacillus gasseri preparation; the phase one is a period of 1-21 days of the broiler chickens, and the phase two is a period of 22-35 days of the broiler chickens; the preservation number of the Bacillus amyloliquefaciens TL is CCTCC NO: M 2015359.

2. The feed additive according to claim 1, characterized in that, The number of viable bacteria in the B. amyloliquefaciens TL preparation is ≥1.0×10 10 CFU / g.

3. The feed additive according to claim 1, characterized in that, The viable cell count in the Lactobacillus gasseri preparation is ≥ 1.0 x 10 9 CFU / kg.

4. The feed additive according to claim 2, characterized in that, The adding amount of the Bacillus amyloliquefaciens TL preparation in the feed is 200 mg / kg.

5. The feed additive according to claim 3, characterized in that, The adding amount of the Lactobacillus gasseri preparation in the feed is 1000 mg / kg.

6. Use of the feed additive according to any one of claims 1-5 in the preparation of a preparation for improving glycolipid metabolism of broiler chickens.

7. Use according to claim 6, characterized in that, The glycolipid metabolism comprises promoting fat synthesis and transport and accelerating gluconeogenesis.

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