Glabridin-containing anti-yellowing and whitening synergistic composition and application thereof in cosmetics

Abstract

The invention belongs to the field of cosmetics, and particularly relates to an anti-yellowing and whitening synergistic composition containing glabridin and application of the anti-yellowing and whitening synergistic composition in cosmetics. The anti-yellowing, whitening and synergistic composition containing glabridin is prepared from tranexamic acid, a peony root extracting solution, glabridin, citric acid and sodium pyrosulfite. The anti-yellowing and whitening synergistic composition containing glabridin provided by the invention has excellent stability, and has the effects of whitening, resisting wrinkles and improving skin elasticity.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of cosmetics, in particular to an anti-yellowing and whitening synergistic composition containing glabridin and its application in cosmetics. BACKGROUND

[0002] Tranexamic acid, also known as aminomethylcyclohexane carboxylic acid or coagulation acid, has the chemical name of trans-4-aminomethylcyclohexane carboxylic acid (molecular formula: C8H 15 NO2), which has the reaction of an amino acid. In Europe, the United States, Japan and China, there are many oral and topical products on the market containing tranexamic acid.

[0003] The whitening mechanism of tranexamic acid is mainly achieved by regulating melanin production and transport in multiple dimensions. Tranexamic acid (used for whitening) is often compounded with other ingredients for synergistic effect, but the common whitening ingredients on the market are prone to react with tranexamic acid, resulting in discoloration and deterioration of the product.

[0004] Moreover, the molecular structure of tranexamic acid contains an amino group and a carboxyl group. The amino group can chelate with certain metal ions (such as iron ions or copper ions), causing color change. Components containing aldehyde groups may undergo condensation reaction with the amino group in tranexamic acid, resulting in discoloration and inactivation of tranexamic acid.

[0005] In addition, it is found in experiments and applications that tranexamic acid is easily decomposed by light and reacts easily with other whitening ingredients. The degradation and discoloration of tranexamic acid can lead to a decrease or loss of product efficacy, and may also cause irritation.

[0006] Therefore, it is necessary to find a way to protect tranexamic acid and maintain its good stability in cosmetic applications. SUMMARY

[0007] The purpose of the present application is to overcome the shortcomings and deficiencies of the prior art and provide an anti-yellowing and whitening synergistic composition containing glabridin and its preparation method and application. The stability of tranexamic acid in cosmetic applications is improved by adjusting the pH value of the composition and adding ingredients with free radical scavenging effect. To achieve the above purpose, the present application provides the following technical solutions.

[0008] In a first aspect, the present application provides an anti-yellowing and whitening synergistic composition containing glabridin.

[0009] An anti-yellowing and whitening synergistic composition containing glabridin, characterized in that it comprises: tranexamic acid, peony root extract, glabridin, citric acid and sodium pyrosulfite.

[0010] In some embodiments, the mass ratio of the citric acid and the extract of Paeonia suffruticosa is (1-4):(0.1-1). In some embodiments, the mass ratio of the citric acid and the extract of Paeonia suffruticosa is 1:0.1-1:0.25. In some embodiments, the mass ratio of the citric acid and the extract of Paeonia suffruticosa is 1:0.1, 1:0.15, 3:0.5, 1:0.2, 1:0.25, or any value within a range between any two of the values.

[0011] In some embodiments, the mass ratio of the citric acid and glabridin is (1-4):(0.03-0.2). In some embodiments, the mass ratio of the citric acid and glabridin is 1:0.025-1:0.05. In some embodiments, the mass ratio of the citric acid and glabridin is 1:0.025, 1:0.03, 3:0.2, 1:0.035, 1:0.04, 1:0.045, 1:0.05, or any value within a range between any two of the values.

[0012] In some embodiments, the mass ratio of the citric acid and citric acid is (1-4):(0.05-0.4). In some embodiments, the mass ratio of the citric acid and citric acid is 1:0.05-1:0.1. In some embodiments, the mass ratio of the citric acid and citric acid is 1:0.05, 1:0.06, 3:0.2, 1:0.07, 1:0.08, 1:0.09, 1:0.1, or any value within a range between any two of the values.

[0013] In some embodiments, the mass ratio of the citric acid and sodium metabisulfite is (1-4):(0.03-0.2). In some embodiments, the mass ratio of the citric acid and sodium metabisulfite is 1:0.03-1:0.05. In some embodiments, the mass ratio of the citric acid and sodium metabisulfite is 1:0.03, 3:0.1, 1:0.035, 1:0.04, 1:0.045, 1:0.05, or any value within a range between any two of the values.

[0014] In some embodiments, the mass ratio of the citric acid, the extract of Paeonia suffruticosa, glabridin, citric acid, and sodium metabisulfite is (1-4):(0.1-1):(0.03-0.2):(0.05-0.4):(0.03-0.2). In some embodiments, the mass ratio of the citric acid, the extract of Paeonia suffruticosa, glabridin, citric acid, and sodium metabisulfite is 1:(0.1-0.25):(0.025-0.05):(0.05-0.1):(0.03-0.05).

[0015] In some embodiments, the mass ratio of the citric acid, the root of tree peony extract, the glabridin, the citric acid and the sodium pyrosulfite is 1:0.1:0.03:0.05:0.03, 4:1:0.2:0.4:0.2, 2:0.3:0.05:0.2:0.1, 3:0.5:0.1:0.2:0.1 or 3:0.75:0.15:0.3:0.15.

[0016] The citric acid is a compound that is easily decomposed under light, and in cosmetic applications, the citric acid can be used to reduce the formation of melanin in the skin, inhibit the transport of melanin granules to the superficial layer of the epidermis, thereby improving the skin color, playing a role in removing freckles and whitening. In addition, the citric acid has repair and anti-inflammatory properties, and can be used to soothe and repair damaged skin due to exposure to ultraviolet light, and to reduce discomfort and erythema caused by sun exposure. However, due to the fact that the citric acid can increase the sensitivity and response of the skin, too high a content of citric acid can cause the skin to be too sensitive, leading to skin discomfort, erythema, allergic reactions and other skin problems. In addition, studies have found that the citric acid will yellow, degrade under light conditions, alkaline conditions and high temperatures; and through testing, the citric acid is most sensitive under light conditions, the yellowing of the product is most obvious, and the amount of degradation of the citric acid is relatively large. Therefore, the present application specifically protects the citric acid in the composition by adding a weak acid with chelating ability to adjust the pH value and adding ingredients with free radical scavenging effect, thereby improving the stability of the citric acid in cosmetic applications.

[0017] The main active ingredient of the root of tree peony extract, paeonol, has significant antioxidant capacity, can effectively scavenge free radicals and inhibit lipid peroxidation. At the same time, it can also help the skin to better resist inflammation and allergies, enhance the skin barrier and keep the skin in a better state by inhibiting the release of histamine and tumor necrosis factor (TNF-alpha), inhibiting the activation of CD 40 mononuclear antibody, recombinant interleukin-4 (ril-4) and recombinant histamine releasing factor (rhrf), and down-regulating the expression of IL-4 in cell activation reverse transcription polymerase chain reaction. Glabridin (English name: Glabridin, CAS number: 59870-68-7) has the ability to scavenge oxygen free radicals similar to superoxide dismutase (SOD), inhibits the generation of active oxygen, and can also synergistically enhance the antioxidant capacity with citric acid; at the same time, as a whitening ingredient, it can act on the epidermal layer, inhibit the activity of tyrosinase, dopachrome tautomerase (TRP-2), DHICA oxidase and alpha-MSH melanocyte-stimulating hormone, thereby inhibiting melanin production and inhibiting melanin transfer, and it can also inhibit the proliferation of melanocytes and B16 cells, thereby helping to reduce the total amount of melanin and supplement the pathways not covered by citric acid in the whitening pathway. As an AHA, citric acid can loosen the intercellular connections of keratinocytes, promote the exfoliation of old and waste keratin, accelerate the renewal of the epidermis, soften the keratin layer, and improve the transdermal absorption rate of subsequent active ingredients (such as tranexamic acid and glabridin); Sodium metabisulfite has strong antioxidant properties and can synergize with the antioxidant capacity of tranexamic acid to enhance the whitening effect achieved through the antioxidant pathway. As a reducing agent, sodium metabisulfite can neutralize free radicals by providing hydrogen atoms, block the chain oxidation reaction of active substances, and delay discoloration and deterioration. In addition, sodium metabisulfite has certain antibacterial ability and can enhance the preservative capacity of the formula. Tranexamic acid is prone to Maillard reaction and discoloration and deterioration under alkaline conditions. Therefore, the present application attempts to adjust the pH value by adding a weak acid to control the pH value in the range of 5.0-6.0, and it is found that the stability of tranexamic acid is significantly improved. Citric acid, as a natural organic acid, is very effective in maintaining a stable pH value and can stabilize the pH value for a long time. In addition, citric acid has good metal ion chelating ability and can effectively prevent iron and copper ions from reacting with tranexamic acid. Therefore, by adding citric acid to control the pH value of the composition containing tranexamic acid, the problem of tranexamic acid yellowing and degradation can be more persistent and effective.

[0018] In a second aspect, the present application provides a preparation method of the anti-yellowing and whitening synergistic composition containing glabridin according to the first aspect.

[0019] A preparation method of the anti-yellowing and whitening synergistic composition containing glabridin according to the first aspect, comprising the following steps: (1) mixing tranexamic acid, peony root extract, and glabridin, heating, and uniformly mixing and dispersing to obtain a mixed solution; (2) cooling the mixed solution obtained in step (1), adding sodium metabisulfite, uniformly mixing and dispersing, and adding citric acid to adjust the pH value, thereby obtaining the anti-yellowing and whitening synergistic composition.

[0020] In some embodiments, the heating of step (1) is heating to 50-60℃. In some embodiments, the heating of step (1) is heating to 50℃, 55℃, 60℃, or any value within a range between any two of these values.

[0021] In some embodiments, the cooling of step (2) is cooling to 42-48℃. In some embodiments, the cooling of step (2) is cooling to 42℃, 43℃, 44℃, 45℃, 46℃, 47℃, 48℃, or any value within a range between any two of these values.

[0022] In some embodiments, the adjusting pH of step (2) is adjusting pH to 5.0-6.0. In some embodiments, the adjusting pH of step (2) is adjusting pH to 5.0, 5.1, 5.2, 5.3, 5.4, 5.5, 5.6, 5.7, 5.8, 5.9, 6.0, or any value within a range between any two of these values.

[0023] In a third aspect, the present application provides a cosmetic product containing ascorbic acid.

[0024] A cosmetic product containing ascorbic acid, which comprises the anti-yellowing, whitening synergistic composition of the first aspect or the anti-yellowing, whitening synergistic composition prepared by the preparation method of the second aspect.

[0025] In some embodiments, the anti-yellowing, whitening synergistic composition is present in the cosmetic product containing ascorbic acid in an amount of 0.27wt%-5.8wt% based on the total mass of the cosmetic product containing ascorbic acid. In some embodiments, the anti-yellowing, whitening synergistic composition is present in the cosmetic product containing ascorbic acid in an amount of 1.21wt%-5.8wt% based on the total mass of the cosmetic product containing ascorbic acid. In some embodiments, the anti-yellowing, whitening synergistic composition is present in the cosmetic product containing ascorbic acid in an amount of 1.21wt%, 1.5wt%, 2wt%, 2.5wt%, 2.6wt%, 2.65wt%, 2.7wt%, 3wt%, 3.5wt%, 3.6wt%, 3.7wt%, 3.8wt%, 3.9wt%, 4wt%, 4.1wt%, 4.2wt%, 4.3wt%, 4.35wt%, 4.4wt%, 4.5wt%, 5wt%, 5.5wt%, 5.8wt%, or any value within a range between any two of these values, based on the total mass of the cosmetic product containing ascorbic acid.

[0026] In some embodiments, the cosmetic product containing ascorbic acid further comprises at least one of an emulsifier, an emollient, a humectant, a thickening agent, a preservative, and water.

[0027] In some embodiments, the cosmetic product containing ascorbic acid further comprises at least one of an emulsifier, an emollient, a humectant, a thickening agent, a preservative, and water.

[0028] In some embodiments, the emulsifier is present in the uric acid-containing cosmetic in an amount of 1 wt% to 5 wt%, based on the total mass of the uric acid-containing cosmetic. In some embodiments, the emulsifier is present in the uric acid-containing cosmetic in an amount of 1 wt%, 2 wt%, 3 wt%, 4 wt%, 5 wt%, or any range between any two of these values, based on the total mass of the uric acid-containing cosmetic. In some embodiments, the emulsifier is present in the uric acid-containing cosmetic in an amount of 2 wt%, based on the total mass of the uric acid-containing cosmetic.

[0029] In some embodiments, the emollient is present in the uric acid-containing cosmetic in an amount of 1 wt% to 20 wt%, based on the total mass of the uric acid-containing cosmetic. In some embodiments, the emollient is present in the uric acid-containing cosmetic in an amount of 1 wt% to 10 wt%, based on the total mass of the uric acid-containing cosmetic. In some embodiments, the emollient is present in the uric acid-containing cosmetic in an amount of 1 wt%, 2 wt%, 3 wt%, 4 wt%, 5 wt%, 6 wt%, 7 wt%, 8 wt%, 9 wt%, 10 wt%, 11 wt%, 12 wt%, 13 wt%, 14 wt%, 15 wt%, 16 wt%, 17 wt%, 18 wt%, 19 wt%, 20 wt%, or any range between any two of these values, based on the total mass of the uric acid-containing cosmetic. In some embodiments, the emollient is present in the uric acid-containing cosmetic in an amount of 5 wt%, based on the total mass of the uric acid-containing cosmetic.

[0030] In some embodiments, the humectant is present in the uric acid-containing cosmetic in an amount of 1 wt% to 10 wt%, based on the total mass of the uric acid-containing cosmetic. In some embodiments, the humectant is present in the uric acid-containing cosmetic in an amount of 1 wt%, 2 wt%, 3 wt%, 4 wt%, 5 wt%, 6 wt%, 7 wt%, 8 wt%, 9 wt%, 10 wt%, or any range between any two of these values, based on the total mass of the uric acid-containing cosmetic. In some embodiments, the humectant is present in the uric acid-containing cosmetic in an amount of 3%, based on the total mass of the uric acid-containing cosmetic.

[0031] In some embodiments, the mass percentage of the thickening agent in the uric acid containing cosmetic is 0.05wt% - 0.5wt% based on the total mass of the uric acid containing cosmetic. In some embodiments, the mass percentage of the thickening agent in the uric acid containing cosmetic is 0.1wt% - 0.5wt% based on the total mass of the uric acid containing cosmetic. In some embodiments, the mass percentage of the thickening agent in the uric acid containing cosmetic is 0.05wt%, 0.1wt%, 0.2wt%, 0.3wt%, 0.4wt%, 0.5wt% or any range between any two of these values based on the total mass of the uric acid containing cosmetic. In some embodiments, the mass percentage of the thickening agent in the uric acid containing cosmetic is 0.2%.

[0032] In some embodiments, the mass percentage of the preservative in the uric acid containing cosmetic is 0.1wt% - 1wt% based on the total mass of the uric acid containing cosmetic. In some embodiments, the mass percentage of the preservative in the uric acid containing cosmetic is 0.1wt%, 0.2wt%, 0.3wt%, 0.4wt%, 0.5wt%, 0.6wt%, 0.7wt%, 0.8wt%, 0.9wt%, 1wt% or any range between any two of these values based on the total mass of the uric acid containing cosmetic. In some embodiments, the mass percentage of the preservative in the uric acid containing cosmetic is 0.5wt% based on the total mass of the uric acid containing cosmetic.

[0033] In some embodiments, the mass percentage of the water in the uric acid containing cosmetic is 67.2wt% - 96.53wt% based on the total mass of the uric acid containing cosmetic. In some embodiments, the mass percentage of the water in the uric acid containing cosmetic is 67.2wt%, 68wt%, 69wt%, 70wt%, 75wt%, 80wt%, 85wt%, 90wt%, 95wt%, 96.53wt% or any range between any two of these values based on the total mass of the uric acid containing cosmetic. In some embodiments, the mass percentage of the water in the uric acid containing cosmetic is 83.5wt% - 88.09wt% based on the total mass of the uric acid containing cosmetic.

[0034] In some embodiments, the total mass percentage of the anti-yellowing, whitening boosting composition, emulsifier, emollient, humectant, thickening agent, preservative and water is 100wt%.

[0035] In some embodiments, the anti-yellowing, whitening-boosting composition is present in the riboflavin-containing cosmetic in an amount of 1.21 wt% to 5.8 wt%, the emulsifier is present in the riboflavin-containing cosmetic in an amount of 1 wt% to 3 wt%, the emollient is present in the riboflavin-containing cosmetic in an amount of 3 wt% to 7 wt%, the humectant is present in the riboflavin-containing cosmetic in an amount of 1 wt% to 5 wt%, the thickening agent is present in the riboflavin-containing cosmetic in an amount of 0.1 wt% to 0.5 wt%, the preservative is present in the riboflavin-containing cosmetic in an amount of 0.2 wt% to 1 wt%, and the remainder is water, based on the total weight of the riboflavin-containing cosmetic.

[0036] In some embodiments, the anti-yellowing, whitening-boosting composition is present in the riboflavin-containing cosmetic in an amount of 1.21 wt% to 5.8 wt%, the emulsifier is present in the riboflavin-containing cosmetic in an amount of 2 wt%, the emollient is present in the riboflavin-containing cosmetic in an amount of 5 wt%, the humectant is present in the riboflavin-containing cosmetic in an amount of 3 wt%, the thickening agent is present in the riboflavin-containing cosmetic in an amount of 0.2 wt%, the preservative is present in the riboflavin-containing cosmetic in an amount of 0.5 wt%, and the remainder is water, based on the total weight of the riboflavin-containing cosmetic.

[0037] In some embodiments, the emulsifier comprises at least one of cetyl stearyl alcohol, cetyl stearyl glucoside, glyceryl stearate, PEG-100 stearate.

[0038] In some embodiments, the emollient comprises at least one of dimethicone, isononyl isononanoate, caprylic / capric triglyceride.

[0039] In some embodiments, the humectant comprises at least one of glycerin, 1,3- butylene glycol, 1,2-hexanediol, dipropylene glycol, diglycerol.

[0040] In some embodiments, the thickening agent comprises at least one of acryloyldimethyltaurinate / VP copolymer, polyacrylate crosspolymer-6, sodium polyacrylate, carbomer, xanthan gum.

[0041] In some embodiments, the preservative comprises at least one of p-hydroxyacetophenone, phenoxyethanol, 1,2-hexanediol.

[0042] In a third aspect, the present application provides a method for preparing the riboflavin-containing cosmetic of the second aspect.

[0043] A preparation method of the orthonic acid-containing cosmetic of the second aspect, comprising the following steps: (S1) mixing an emulsifier and an emollient, heating and uniformly dispersing the mixture to obtain a mixture A; (S2) mixing a humectant, a preservative, a thickening agent, water, and the mixture A obtained in step S1, heating and uniformly dispersing the mixture; vacuum defoaming, stirring and cooling to obtain a mixture B; (S3) mixing the anti-yellowing and whitening synergistic composition containing glycyrrhizin with the mixture B obtained in step S2, and uniformly dispersing the mixture; (S4) cooling, thereby obtaining the orthonic acid-containing cosmetic.

[0044] In some embodiments, the heating in step S1 is heating to 80-85°C. In some embodiments, the heating in step S1 is heating to 80°C, 81°C, 82°C, 83°C, 84°C, 85°C, or any value within a range between any two of the values.

[0045] In some embodiments, the heating in step S2 is heating to 80-85°C. In some embodiments, the heating in step S2 is heating to 80°C, 81°C, 82°C, 83°C, 84°C, 85°C, or any value within a range between any two of the values.

[0046] In some embodiments, the cooling in step S2 is cooling to 45-48°C. In some embodiments, the cooling in step S2 is cooling to 45°C, 46°C, 47°C, 48°C, or any value within a range between any two of the values.

[0047] In some embodiments, the temperature of the incubation and mixing in step S3 is 45-48°C. In some embodiments, the temperature of the incubation and mixing in step S3 is 45°C, 46°C, 47°C, 48°C, or any value within a range between any two of the values.

[0048] In some embodiments, the cooling in step S4 is cooling to 25-42°C. In some embodiments, the cooling in step S4 is cooling to 25°C, 30°C, 35°C, 38°C, 40°C, 42°C, or any value within a range between any two of the values.

[0049] In a fourth aspect, the present application provides a use of the anti-yellowing and whitening synergistic composition or the anti-yellowing and whitening synergistic composition prepared by the preparation method.

[0050] A use of the anti-yellowing and whitening synergistic composition of the first aspect or the anti-yellowing and whitening synergistic composition prepared by the preparation method of the second aspect in preparing a cosmetic.

[0051] In some embodiments, the cosmetic is used for whitening, freckle-removing, anti-wrinkle, repairing and / or firming.

[0052] Advantages Compared with the prior art, the embodiment of the present application has at least one of the following advantages: (1) The composition and the cosmetic prepared by selecting the specific component proportion have better light stability, and better whitening, anti-wrinkle and skin elasticity improvement effects.

[0053] (2) Compared with the composition and the cosmetic lacking one, two, three or four of the peony root extract, glabridin, citric acid and sodium pyrosulfite, the composition and the cosmetic of the present application simultaneously containing the peony root extract, glabridin, citric acid and sodium pyrosulfite are more conducive to improving the light stability of the composition and the cosmetic, and the peony root extract, glabridin, citric acid and sodium pyrosulfite synergistically improve the light stability of the composition and the cosmetic, which has an unexpected synergistic technical effect.

[0054] (3) Compared with the proportion of the peony root extract outside the proportion range provided by the present application, the present application preferably adopts the mass ratio of the taurine and the peony root extract as (1-4):(0.1-1) or 1:0.1-1:0.25, which is more conducive to improving the light stability of the composition and the cosmetic and improving the skin elasticity, whitening and anti-inflammatory effects, and has an unexpected synergistic technical effect.

[0055] (4) Compared with the proportion of the peony root extract outside the proportion range provided by the present application, the present application preferably adopts the mass ratio of the taurine and the glabridin as (1-4):(0.03-0.2) or 1:0.025-1:0.05, which is more conducive to improving the light stability of the composition and the cosmetic and improving the skin elasticity, whitening and anti-inflammatory effects, and has an unexpected synergistic technical effect.

[0056] (5) Compared with the proportion of the peony root extract outside the proportion range provided by the present application, the present application preferably adopts the mass ratio of the taurine and the citric acid as (1-4):(0.05-0.4) or 1:0.05-1:0.1, which is more conducive to improving the light stability of the composition and the cosmetic and improving the skin elasticity, whitening and anti-inflammatory effects, and has an unexpected synergistic technical effect.

[0057] (6) Compared with the proportion of the peony root extract outside the proportion range provided by the present application, the present application preferably adopts the mass ratio of the taurine and the sodium pyrosulfite as (1-4):(0.03-0.2) or 1:0.03-1:0.05, which is more conducive to improving the light stability of the composition and the cosmetic and improving the skin elasticity, whitening and anti-inflammatory effects, and has an unexpected synergistic technical effect.

[0058] (7) Compared with the comparative example 6 in which citric acid is replaced by sodium dihydrogen phosphate, the present application uses citric acid to adjust the pH of the composition to be weakly acidic, which is more conducive to improving the light stability of the obtained composition and cosmetic and enhancing the skin elasticity, whitening and anti-inflammatory effects, and has an unexpected synergistic technical effect.

[0059] Terminology In the description of the present application, the meaning of "a plurality of" is two or more than two, unless otherwise explicitly specified.

[0060] The term "room temperature" means ambient temperature, which refers to a temperature of about 10°C to about 30°C, or about 20°C to 30°C, or about 25°C.

[0061] The term "wt%" means mass percentage.

[0062] The term "%vol" means volume percentage.

[0063] The term "balance" means the remaining part used to make up the overall proportion to 100wt% after the proportions of all other ingredients in the composition or preparation product have been explicitly specified.

[0064] In the description of the present application, the description of the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" and the like means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present application. In the present description, the illustrative description of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any appropriate manner in any one or more embodiments or examples. In addition, the skilled person in the art can combine and combine the different embodiments or examples described in the present description and the features of the different embodiments or examples without contradiction.

[0065] In the following, all the numbers disclosed herein are approximate, regardless of whether the word "about" or "approximately" is used. The value of each number can vary by 1%, 2%, 5%, 7%, 8%, 10%, 15% or 20%. Whenever a number with a value of N is disclosed, any number with a value of N + / - 1%, N + / - 2%, N + / - 3%, N + / - 5%, N + / - 7%, N + / - 8%, N + / - 10%, N + / - 15% or N + / - 20% is explicitly disclosed, wherein "+" or "-" means plus or minus. DETAILED DESCRIPTION

[0066] In order to make the technical scheme of the present application better understood by the skilled in the art, some non-limiting embodiments are further disclosed below, and the present application is further described in detail.

[0067] The reagents used in the present application can be purchased from the market or prepared by the method described in the present application.

[0068] Firstly, the sources of some reagents used in the embodiments or comparative examples of the present application are as follows: Peony root extract: trade name: Essenpure PRE; purchased from Zhejiang Chenhai Life Science Co., Ltd. The raw material production process is as follows: the peony root is peeled and stripped, then crushed, glycerol and water are added and stirred for extraction, the insoluble substances are removed by filtration, and the filtrate is obtained. Then, 0.2%-0.6% p-hydroxyacetophenone and 0.5%-2% 1,2-hexanediol are added and uniformly mixed, and the filtrate is obtained. The content of water is 50wt%-80wt%, the content of glycerol is 12wt%-25wt%, the content of peony root (PAEONIA SUFFRUTICOSA) extract is 6wt%-35wt%, the content of p-hydroxyacetophenone is 0.2wt%-0.6wt%, and the content of 1,2-hexanediol is 0.5wt%-2wt%.

[0069] Glycyrrhizin: purchased from Guangzhou Qingnang Biological Technology Co., Ltd.

[0070] Acryloyldimethyltaurinate / VP copolymer: 2-acrylamide-2-methylpropane sulfonic acid (AMPS) and vinyl pyrrolidone are reacted in the presence of ammonia and a crosslinking agent; trade name: Aristoflex AVC; purchased from Clariant Chemicals (Shanghai) Co., Ltd.

[0071] MONTANOV 68 MB: containing 75%-85% cetylstearyl alcohol and 15%-25% cetylstearyl glucoside. Manufacturer: SEPPIC S.A. II. Detection method of histidine content The histidine content is determined by high performance liquid chromatography (HPLC). The HPLC conditions are as follows: column: Hypersil ODS C 18 column, 5μm, 150mm x 4.6mm (inner diameter); column temperature: 30℃; mobile phase: 0.1%vol formic acid acetonitrile solution and 0.1%vol formic acid aqueous solution; flow rate: 1.0mL / min; detection wavelength: 250nm; sample injection amount: 20μL.

[0072] Formulations 1-5 and comparative formulations 1-19: preparation of compositions and formulation screening investigation 1. Formulation 1-Formulation 5: preparation of compositions Formulation: see Table 1.

[0073] Table 1: Composition Formulation

[0074] Preparation method of Prescription 1~Prescription 5: (1) Take the aminic acid, peony root extract and glabridin, heat to 50℃, mix and disperse uniformly to obtain a mixed solution; (2) Cool the mixed solution obtained in step (1) to 48℃, add sodium pyrosulfite, mix and disperse uniformly, add citric acid to adjust the pH value to 5.0-6.0, and the composition is obtained.

[0075] 2, Comparative Prescription 1~Comparative Prescription 5: Missing ingredient investigation Formulation: see Table 2.

[0076] Table 2: Composition Formulation

[0077] Preparation method of Comparative Prescription 1~Comparative Prescription 5: Refer to the preparation method of Prescription 1~Prescription 5, the difference is that the ingredients with 0 weight parts are not added in the formulation.

[0078] 3, Comparative Prescription 6: Acid-base investigation Formulation: see Table 3.

[0079] Table 3: Composition Formulation

[0080] Preparation method of Comparative Prescription 6: (1) Take the aminic acid, peony root extract and glabridin, heat to 50℃, mix and disperse uniformly to obtain a mixed solution; (2) Cool the mixed solution obtained in step (1) to 48℃, add sodium pyrosulfite, mix and disperse uniformly, add sodium dihydrogen phosphate to adjust the pH value, and the composition is obtained.

[0081] 4, Comparative Prescription 7~Comparative Prescription 9: Ingredient ratio investigation Formulation: see Table 4.

[0082] Table 4: Composition Formulation

[0083] Preparation method of Comparative Prescription 7~Comparative Prescription 9: (1) Take the aminic acid, peony root extract and glabridin, heat to 50℃, mix and disperse uniformly to obtain a mixed solution; (2) The mixture obtained in step (1) is cooled to 48℃, sodium metabisulfite is added, and mixed and dispersed uniformly, and citric acid is added, to obtain the composition.

[0084] 5. Comparative Formulation 10-Comparative Formulation 13: Investigation of missing ingredients Formulation: see Table 5.

[0085] Table 5: Composition Formulation

[0086] Preparation method: refer to the preparation method of Formulation 1-Formulation 5, except that the ingredients with a weight fraction of 0 are not added.

[0087] 6. Comparative Formulation 14-Comparative Formulation 19: Investigation of missing ingredients Formulation: see Table 6.

[0088] Table 6: Composition Formulation

[0089] Preparation method: refer to the preparation method of Formulation 1-Formulation 5.

[0090] Example 1-Example 5, Comparative Example 1-Comparative Example 9: Preparation of cosmetics Formulation: see Table 9, Table 10 and Table 11.

[0091] Table 7: Formulation of Example 1-Example 5

[0092] Table 10: Formulation of Comparative Example 1-Comparative Example 6

[0093] Table 11: Formulation of Comparative Example 7-Comparative Example 9

[0094] Preparation method: (S1) Mix the emulsifier and emollient, heat to 80℃, mix and disperse uniformly, to obtain mixture A; (S2) Mix the humectant, preservative, thickening agent, water, and mixture A obtained in step S1, heat to 80℃, mix and disperse uniformly; vacuum defoam, stir and cool to 48℃, to obtain mixture B; (S3) Mix the anti-yellowing, whitening synergistic composition containing glabridin with mixture B obtained in step S2, and disperse uniformly; (S4) After cooling to 38-42℃, discharge, to obtain the cosmetic containing the ascorbic acid.

[0095] Test Example 1: Investigation of stability of the composition The compositions obtained in Formulation 1-Formulation 5 and Comparative Formulation 1-Comparative Formulation 17 were placed in a transparent container under light conditions (1000 Lux) for 15 days, and the retention rate of the aminopicric acid was detected.

[0096] The specific method is as follows: the sample of the composition to be tested was placed in a transparent plastic bottle and placed in a light incubator at 25°C, and the light cycle of the incubator was 24:0 (light / dark; 1000 Lux). The aminopicric acid content of Formulation 1-5 and Comparative Formulation 1-17 was tested every day, and the test was performed for 15 days.

[0097] The results are shown in Table 13.

[0098] Table 13: Aminopicric acid retention rate results (unit: %)

[0099] Note: Aminopicric acid retention rate (%) after 15 days of light = (aminopicric acid content after 15 days of light ÷ aminopicric acid content at 0 days) x 100% Conclusion: (1) The composition containing the peony root extract, glabridin, citric acid and sodium pyrosulfite provided by the present application has excellent light stability, and the aminopicric acid is not substantially degraded under light conditions.

[0100] (2) Compared with the absence of one, two, three or four of the peony root extract, glabridin, citric acid and sodium pyrosulfite, the present application simultaneously contains the peony root extract, glabridin, citric acid and sodium pyrosulfite, which is more conducive to improving the light stability of the obtained composition. The peony root extract, glabridin, citric acid and sodium pyrosulfite synergistically improve the stability of aminopicric acid, and have unexpected synergistic technical effects.

[0101] (3) Compared with the proportion of the peony root extract provided by the present application outside the proportion range, the present application preferably uses a mass ratio of aminopicric acid to peony root extract of (1-4):(0.1-1) or 1:0.1-1:0.25, which is more conducive to improving the light stability of the obtained composition, and has unexpected synergistic technical effects.

[0102] (4) Compared with the proportion of the peony root extract provided by the present application outside the proportion range, the present application preferably uses a mass ratio of aminopicric acid to glabridin of (1-4):(0.03-0.2) or 1:0.025-1:0.05, which is more conducive to improving the light stability of the obtained composition, and has unexpected synergistic technical effects.

[0103] (5) Compared with the proportion of the peony root extract provided by the present application outside the proportion range, the present application preferably adopts the mass ratio of the citric acid and the tartaric acid as (1-4):(0.05-0.4) or 1:0.05-1:0.1, which is more conducive to improving the light stability of the obtained composition and has an unexpected synergistic technical effect.

[0104] (6) Compared with the proportion of the peony root extract provided by the present application outside the proportion range, the present application preferably adopts the mass ratio of the citric acid and the tartaric acid as (1-4):(0.03-0.2) or 1:0.03-1:0.05, which is more conducive to improving the light stability of the obtained composition and has an unexpected synergistic technical effect.

[0105] (7) Compared with the comparative example 6 in which the citric acid is replaced by sodium dihydrogen phosphate, the present application adopts the citric acid to adjust the pH of the composition to be weakly acidic, which is more conducive to improving the light stability of the obtained composition and has an unexpected synergistic technical effect.

[0106] Test Example 2: Investigation of the stability of the cosmetic The above effect examples take the cosmetics containing tartaric acid prepared in the above examples 1-5 and comparative examples 1-9 as samples, determine the tartaric acid content in the cosmetics, simulate the degradation of tartaric acid in the cosmetics during use by light conditions (1000 Lux), and test the inhibition effect of the present application on the light degradation of tartaric acid, and the specific method is as follows: The samples are placed in transparent plastic bottles and placed in a 25℃ light incubator, and the light cycle of the incubator is 24:0 (light / dark). The tartaric acid content of examples 1-5 and comparative examples 1-9 is tested every day, and the test is carried out for 15 days.

[0107] The test results of the tartaric acid retention rate are shown in Table 14.

[0108] Table 14: Tartaric acid retention rate results (unit: %)

[0109] Note: Tartaric acid retention rate (%) after 15 days of light = (tartaric acid content after 15 days of light ÷ tartaric acid content on the first day) x 100% Conclusion: (1) As can be seen from Table 14, the natural organic acid citric acid with chelation effect, the peony root extract, glabridin, and sodium metabisulfite (the mass ratio of the four components is (1-4):(0.1-1):(0.03-0.2):(0.05-0.4):(0.03-0.2)) are added in the preferred range, which can effectively protect the cysteine, significantly improve the problem of content reduction of cysteine due to photodegradation, and enhance the stability of cysteine in the composition or cosmetic.

[0110] (2) As can be seen from the comparison of the examples and the comparative examples 1-5, when the peony root extract, glabridin, citric acid, and sodium metabisulfite are not added or only any three of the four components are added, the content of cysteine in the cosmetic is significantly lower than that of the examples, which indicates that the four components interact with each other and synergistically enhance the effect of inhibiting the photodegradation of cysteine, significantly improve the stability of cysteine in the composition or cosmetic, and have unexpected synergistic technical effects.

[0111] (3) In the comparative example 6, sodium dihydrogen phosphate is used to replace citric acid to control the pH value to be greater than 6.0, and it is found that the light stability of the obtained cosmetic is significantly deteriorated, which indicates that the pH value (5.0-6.0) of the composition adjusted by the present application is more conducive to improving the light stability of glabridin in the composition or cosmetic, and has unexpected technical effects.

[0112] (4) In the comparative examples 7-9, the content of the peony root extract, glabridin, and sodium metabisulfite exceeds the preferred range of the present application, and the content of cysteine in the cosmetic is also lower than that of the examples.

[0113] (5) The above results fully demonstrate that the four components, i.e., the peony root extract, glabridin, citric acid, and sodium metabisulfite, can synergistically inhibit the photodegradation of cysteine at a specific mass ratio, significantly enhance the stability of cysteine in the cosmetic, and have unexpected synergistic technical effects.

[0114] Effect Example 3: Investigation of skin elasticity, whitening, and anti-inflammatory efficacy In this effect example, the cosmetic containing cysteine prepared in the above examples 1-5 and comparative examples 1-9 is used as a sample, and the whitening efficacy and skin elasticity conditioning are evaluated, and the specific method is as follows.

[0115] 1. Instruments and samples Instrument: Visia-CR (Canfield, USA); Skin melanin and hemoglobin test probe Mexameter MX18 (Courage + Khazaka, Germany); Image-Pro skin comprehensive analysis software (Media Cybernetics).

[0116] Samples: 14 cosmetic samples of Examples 1-5 and Comparative Examples 1-9.

[0117] 2. Selection of subjects Eligible volunteers were selected and signed informed consent forms to ensure that a total of 140 people completed the trial, with 10 volunteers for each sample.

[0118] Inclusion criteria: healthy female volunteers with daily habits of using skin care products, Chinese, aged 35-45; volunteers with periorbital fine lines / wrinkles on their faces, and not sensitive to commonly used cosmetics; high compliance of subjects.

[0119] Exclusion criteria: subjects who have used antihistamines in the past week or immunosuppressants in the past month; subjects who have used any anti-inflammatory drugs on the test site in the past two months; insulin-dependent diabetic patients; patients with respiratory diseases undergoing treatment; people with allergic constitution, allergic dermatitis, etc., and those with a history of skin diseases; people undergoing dermatological treatment or taking light acid, whitening, and anti-aging drugs in the past month; and those with large areas of test skin features such as birthmarks, scratches, white spots, pigmented nevi, scar nodules, etc. that affect the test.

[0120] 3. Test environmental conditions: the test environment temperature was 20-22°C, the humidity was 40-60%, and real-time dynamic monitoring was performed.

[0121] 4. Product usage method: 10 groups of people received samples of examples and comparative examples. The amount and frequency of use of the skin care product was 0.5g / time, 2 times / day (morning and evening). During the test period, the subjects avoided excessive ultraviolet exposure.

[0122] 5. Test process (1) VISIA CR facial photography A. Skin color analysis: before use and at 4 weeks after use of the sample, clean the face with a reference cleanser, sit in a constant temperature and humidity room for 20 minutes, then take a VISIA CR facial photograph, and select the corresponding test area on the left and right sides of the face for skin color analysis to calculate the ITA°.

[0123] B. Wrinkle score: At 4 weeks before and after the use of the sample, the VISIA CR facial photograph was taken after 20 minutes of sitting in the constant temperature and humidity chamber after cleaning with the facial cleansing reference product. The effect was evaluated according to the numerical value of the VISIA tester, and the numerical value of the wrinkle score of the left side (around the left eye) and the right side (around the right eye) was selected.

[0124] (2) Skin elasticity test Open the Cutometer Q software, click the toolbar Measurement or directly click the Measurement button in the upper right corner, after the pump starts, take off the probe cover, click OK, and gently contact the probe vertically to the skin after cleaning or applying the sample, etc. within 5s countdown time, the probe tip spring is fully retracted, the test starts, and the probe is removed until the test cycle time ends, and the probe cover is covered.

[0125] (3) Whitening and anti-inflammatory effect test: At 4 weeks before and after the use of the sample, the VISIA CR facial photograph was taken after 20 minutes of sitting in the constant temperature and humidity chamber after cleaning with the facial cleansing reference product. The effect was evaluated according to the numerical value of the VISIA tester, and the numerical value of the wrinkle score of the left side (around the left eye) and the right side (around the right eye) was selected.

[0126] 6. Result calculation and analysis: The output data is the average value of 10 people in each group, and the test results are shown in Tables 15-18.

[0127] Table 15: Skin ITA° test results

[0128] Table 16: Change of wrinkle score

[0129] Table 17: Skin elasticity test results

[0130] Table 18: Melanin and red pigment content

[0131] Conclusion: (1) According to the results of Example 3, compared with the tannic acid cosmetic lacking any one of the peony root extract, glabridin, citric acid and sodium pyrosulfite, the tannic acid cosmetic of the present application simultaneously using peony root extract, glabridin, citric acid and sodium pyrosulfite is more conducive to improving the skin elasticity, whitening and anti-inflammatory effect of the obtained composition or cosmetic, and has unexpected technical effect.

[0132] (2) Compared with other pH of the composition, the pH (5.0-6.0) provided by the application is more conducive to improving the skin elasticity, whitening and anti-inflammatory effects of the obtained composition or cosmetic, and has unexpected technical effects.

[0133] (3) Compared with other content ranges of the composition of the strachin, peony root extract, glabridin, citric acid and sodium pyrosulfite, the preferred content range (the mass ratio of the strachin, peony root extract, glabridin, citric acid and sodium pyrosulfite is preferably (1-4):(0.1-1):(0.03-0.2):(0.05-0.4):(0.03-0.2)) is more conducive to improving the skin elasticity, whitening and anti-inflammatory effects of the obtained composition or cosmetic, and has unexpected technical effects.

[0134] The method of the application has been described by preferred embodiments, and the related personnel can obviously modify or appropriately change and combine the method and application described herein to realize and apply the technical effects of the application within the content, spirit and scope of the application. The skilled in the art can refer to the content herein to appropriately improve the process parameters for implementation. It should be particularly pointed out that all similar substitutions and modifications are obvious to the skilled in the art, and they are all considered to be included in the application.

Claims

1. A lightening, anti-yellowing, whitening synergistic composition comprising glycyrrhizin, characterized in that, comprising: trans-amic acid, peony root extract, glabridin, citric acid and sodium pyrosulfite.

2. The anti-yellowing and whitening synergistic composition comprising glabridin according to claim 1, wherein the mass ratio of the trans-amic acid and the peony root extract is (1-4):(0.1-1); and / or the mass ratio of the trans-amic acid and the glabridin is (1-4):(0.03-0.2); and / or the mass ratio of the trans-amic acid and the citric acid is (1-4):(0.05-0.4); and / or the mass ratio of the trans-amic acid and the sodium pyrosulfite is (1-4):(0.03-0.2).

3. The anti-yellowing and whitening synergistic composition comprising glabridin according to claim 1, wherein the mass ratio of the trans-amic acid and the peony root extract is 1:0.1-1:0.25; and / or the mass ratio of the trans-amic acid and the glabridin is 1:0.025-1:0.05; and / or the mass ratio of the trans-amic acid and the citric acid is 1:0.05-1:0.1; and / or the mass ratio of the trans-amic acid and the sodium pyrosulfite is 1:0.03-1:0.

05.

4. The anti-yellowing and whitening synergistic composition comprising glabridin according to any one of claims 1-2, wherein the mass ratio of the trans-amic acid, the peony root extract, the glabridin, the citric acid and the sodium pyrosulfite is (1-4):(0.1-1):(0.03-0.2):(0.05-0.4):(0.03-0.2).

5. The anti-yellowing and whitening synergistic composition comprising glabridin according to any one of claims 1-2, wherein the mass ratio of the trans-amic acid, the peony root extract, the glabridin, the citric acid and the sodium pyrosulfite is 1:(0.1-0.25):(0.025-0.05):(0.05-0.1):(0.03-0.05).

6. The anti-yellowing, whitening synergistic glycyrrhizin-containing composition of claim 1, wherein the mass ratio of the trans-amic acid, the peony root extract, the glabridin, the citric acid and the sodium pyrosulfite is 1:0.1:0.03:0.05:0.03, 4:1:0.2:0.4:0.2, 2:0.3:0.05:0.2:0.1, 3:0.5:0.1:0.2:0.1 or 3:0.75:0.15:0.3:0.

15.

7. A process for the preparation of an anti-yellowing, whitening synergistic composition comprising glabridin according to any one of claims 1 to 6, characterized in that, comprising the following steps: (1) mixing the trans-amic acid, the peony root extract and the glabridin, heating, mixing and dispersing uniformly to obtain a mixture; (2) cooling the mixture obtained in step (1), adding the sodium pyrosulfite, mixing and dispersing uniformly, adding the citric acid to adjust the pH value to obtain the anti-yellowing and whitening synergistic composition.

8. The preparation method according to claim 7, wherein the heating in step (1) is heating to 50-60℃; and / or the cooling in step (2) is cooling to 42-48℃.

9. A cosmetic containing putrescine, characterized in that, the anti-yellowing and whitening synergistic composition comprising glabridin according to any one of claims 1-6 or the anti-yellowing and whitening synergistic composition comprising glabridin prepared by the preparation method according to any one of claims 7-8.

10. Use of the anti-yellowing and whitening synergistic composition comprising glabridin according to any one of claims 1-6 or the anti-yellowing and whitening synergistic composition comprising glabridin prepared by the preparation method according to any one of claims 7-8 in the preparation of cosmetics. Optionally, the cosmetic product is for whitening, spot-removing, anti-wrinkle, repairing and / or firming.

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