Rosemary extract as well as preparation method and application thereof in cosmetics

Abstract

The invention relates to a rosemary extract, a preparation method thereof and application of the rosemary extract in cosmetics, and the preparation method of the rosemary extract comprises the steps of pretreatment, infiltration and solubilization, complex enzyme-pulsed ultrasound synergistic extraction, separation and purification, and concentration and drying. The preparation method of the rosemary extract has the advantages of high efficiency, safety, environmental protection, economy and the like, and the prepared rosemary extract is light in color and high in purity of active ingredients; according to the cosmetic disclosed by the invention, the high-activity rosemary extract prepared by the method is scientifically compounded with a specifically compatible barrier repairing component and other natural anti-inflammatory components, so that a remarkable synergistic interaction effect can be generated, and the obtained cosmetic has good moisturizing, soothing, repairing and anti-oxidation effects and is suitable for nursing sensitive skin.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of daily chemicals, and particularly relates to a rosemary extract, a preparation method thereof and application thereof in cosmetics. BACKGROUND

[0002] With the increasing demand of consumers for the efficacy and safety of skin care products, natural plant extracts are increasingly widely used in cosmetics. Rosemary (Rosmarinus officinalis L.) is considered as an excellent natural antioxidant source due to its high content of rosemary acid, carnosic acid, carnosol and other active antioxidant and anti-inflammatory ingredients, and has great application potential in the field of skin care products.

[0003] At present, the mainstream method for extracting active ingredients of rosemary in industry has obvious defects: (1) water extraction method: high extraction temperature (usually > 90℃), which can cause decomposition and inactivation or volatilization of heat-sensitive components (such as some terpenes and volatile essential oils) in rosemary. At the same time, the extraction efficiency of liposoluble components (such as carnosic acid) is very low, and a large amount of water-soluble impurities such as polysaccharides, proteins and pigments are dissolved, which brings difficulties to subsequent purification, and the final product has dark color which may affect the appearance of the product. (2) Organic solvent method: although the extraction efficiency of liposoluble components is higher, there is an inevitable risk of solvent residue, which may cause skin irritation, which is contrary to the concept of pursuing "natural and safe" skin care, and this method has high energy consumption and great environmental pressure, which is not in line with the development direction of green chemistry. In addition, single emerging technology assisted extraction also has obvious defects: (1) Ultrasonic assisted extraction: although some literatures have reported that ultrasonic can use cavitation effect to break cell wall and improve efficiency, but if used alone, the selectivity of target components in complex plant matrix is still insufficient. More importantly, if continuous high-intensity ultrasound is used, the heat effect and mechanical shear force may cause the inactivation of endogenous enzymes and added enzymes, and the risk of high temperature still exists. (2) Enzyme assisted extraction: the conditions are mild, and it can specifically decompose cell wall structure (such as cellulose and pectin), but it takes a long time, the extraction efficiency is limited, and the release effect of intracellular liposoluble components is not good.

[0004] In summary, there is a obvious contradiction in the prior art: on the one hand, the simple low-temperature extraction method (such as single enzyme method) has low efficiency; on the other hand, the efficient extraction method (such as organic solvent method and subcritical extraction method) has safety risk or cannot avoid the damage of heat-sensitive components caused by high temperature. And the simple combination of ultrasonic and enzyme method also faces the technical risk of enzyme inactivation caused by ultrasonic. Therefore, there is an urgent need in the field for a green extraction method which can truly extract water-soluble (such as rosemary acid) and liposoluble (such as carnosic acid and carnosol) active ingredients in rosemary at low temperature and without organic solvent, with high efficiency and high selectivity, and can maximize the retention of bioactive ingredients. SUMMARY

[0005] The technical problem solved by the present application is to provide a rosemary extract, a preparation method thereof and an application thereof in cosmetics. The preparation method of the rosemary extract of the present application uses composite biological enzymes and specific mode pulsed ultrasonic waves to synergistically act, realizes efficient and directional wall breaking of rosemary cell walls in a low-temperature aqueous system, synchronously extracts water-soluble and fat-soluble active ingredients, and ensures high activity and high quality of the extract through a post-processing process. In addition, the high-activity rosemary extract prepared by the above method is scientifically compounded with specific barrier repair ingredients (such as squalane, erythritol, ceramide NP) and other natural anti-inflammatory ingredients (such as centella asiatica extract) to develop a sensitive skin suitable cosmetic product with significant synergistic effects in terms of moisturizing, soothing, repairing and antioxidant.

[0006] The technical solution adopted by the present application to solve the above problems is as follows:

[0007] In one aspect, the present application provides a preparation method of rosemary extract, comprising the following steps:

[0008] S1. Pre-treatment: taking dry rosemary leaves, performing microwave stabilization treatment under low-temperature conditions, and then crushing the rosemary leaves into rosemary powder with a particle size of 80-100 mesh;

[0009] The microwave stabilization treatment in this step aims to inhibit enzyme activity and reduce degradation of components in subsequent processing.

[0010] S2. Soaking and solubilizing: adding the rosemary powder into a purified water solution containing a natural surfactant, soaking to obtain a mixture;

[0011] The purpose of this step is to preliminarily soak the plant tissue to soften it, preliminarily extract water-soluble components, and create conditions for subsequent extraction of fat-soluble components by adding a mild natural surfactant.

[0012] S3. Composite enzyme-pulsed ultrasonic synergistic extraction: cooling and adjusting the pH of the mixture, adding composite biological enzymes, and immediately performing pulsed ultrasonic treatment to obtain an enzymatic hydrolysate after enzyme inactivation; wherein the composite biological enzymes are cellulase, pectinase and lipase;

[0013] This step is the core of the present application. By using composite biological enzymes in combination with pulsed ultrasonic waves, the cavitation effect and mechanical action of the ultrasonic waves can greatly enhance the contact between the enzymes and the mixture, accelerate the cell wall breaking, and the pulsed ultrasonic mode perfectly avoids the risk of enzyme inactivation while ensuring the cell breaking efficiency, thereby greatly improving the extraction rate and speed of target components such as rosemary acid and salvia acid at low temperature.

[0014] S4. Separation and purification: centrifuging the enzymatic hydrolysate, decolorizing the supernatant to obtain a clear extract;

[0015] The purpose of this step is to separate the active substances in rosemary powder and remove pigment impurities.

[0016] S5. Concentration and drying: the clear extract is concentrated by rotation and dried to obtain rosemary extract powder.

[0017] The preparation method of the rosemary extract of the present application obtains a light yellow to white rosemary extract powder.

[0018] The preparation method of the rosemary extract of the present application successfully solves the problem that pure water cannot extract liposoluble components such as salvia acid by adding a mild natural surfactant and lipase; by using composite enzyme-pulse ultrasonic synergistic extraction, the efficiency of cell disruption is ensured while the risk of enzyme inactivation is perfectly avoided, thereby greatly improving the extraction rate and extraction speed of target components such as rosemary acid and salvia acid at low temperature.

[0019] Further, in step S1, the low temperature is 48-50℃; the microwave stabilization treatment time is 5min; and the pulverization is performed by a high-speed shearing pulverizer.

[0020] Further, in step S2, the mass of the rosemary powder to the volume of the purified water solution containing the natural surfactant is 1:10-15; the temperature of the purified water solution containing the natural surfactant is 60-70℃, wherein the mass concentration of the natural surfactant is 0.1-0.3%; the soaking time is 30-40min; and preferably, the natural surfactant is alkyl glycoside APG0810.

[0021] Further, in step S3, the temperature is reduced to 45-50℃, and the pH is adjusted to 4.5-5.5; the addition amount of the composite biological enzyme is 0.3-3.0% of the mass of the mixture; the mass ratio of cellulase, pectinase and lipase in the composite biological enzyme is (0.8-1.2):(0.8-1.2):(0.4-0.6), preferably 1:1:0.5; in step S3, the parameters of the pulse ultrasonic treatment are: power 200-300W, frequency 40kHz, working cycle 5s on / 5s off, and total treatment time 30-40min; and the enzyme inactivation is to rapidly heat the mixture to 85-90℃ for 5min after enzymolysis, and then reduce the temperature to 50-65℃.

[0022] Further, in the step S4, the centrifugation is performed at a speed of 8000-10000 rpm for 15 min, and the decolorization is performed by circulating the supernatant through a decolorization column filled with activated carbon for 40-60 min.

[0023] Further, in the step S5, the rotary concentration is performed by rotary evaporation under a reduced pressure of 60-65℃ and -0.08--0.1 MPa to obtain a paste with a relative density of 1.10-1.20; and the drying is performed by vacuum freeze drying or spray drying with an inlet temperature of ≤65℃.

[0024] In another aspect, the present application also provides a rosemary extract prepared by the above preparation method.

[0025] The rosemary extract prepared by the present application has high purity and can effectively extract water-soluble and fat-soluble active ingredients, and the extract has light color, and is more easily applied in high-end transparent texture cosmetics.

[0026] In another aspect, the present application also provides a cosmetic comprising the above rosemary extract.

[0027] Further, the cosmetic comprises the following components in mass percentage: the rosemary extract 2.0-4.0%, the extract of centella asiatica 1.0-2.0%, erythritol 3.0-5.0%, ceramide NP 0.05-0.2%, squalane 3.0-5.0%, a humectant 3-8%, an emulsifier 1-4%, a thickening stabilizer 0.1-0.5%, an emollient 1-5%, a pH regulator 0.001-1%, a preservative 0.5-1.5%, a chelating agent 0.02-0.1%, and the balance of deionized water.

[0028] The cosmetic of the present application has a significant synergistic effect in moisturizing, soothing, repairing and antioxidant by scientifically compounding the high-activity rosemary extract prepared by the above method with barrier repair ingredients (such as squalane, erythritol, ceramide NP) and other natural anti-inflammatory ingredients (such as the extract of centella asiatica), and is suitable for sensitive skin.

[0029] Further, the humectant is at least one of glycerin, butylene glycol, panthenol, sugar isomerate; the emulsifier is at least one of hydrogenated lecithin, cetearyl glucoside, PEG-100 stearate, cetearyl olivate, sorbitan olivate; the thickening stabilizer is at least one of carbomer, xanthan gum, acryloyldimethyltaurine ammonium / VP copolymer, magnesium aluminum silicate; the emollient is at least one of dimethicone, caprylic / capric triglyceride, C15-19 alkane, isononyl isononanoate; the pH regulator is at least one of triethanolamine, sodium hydroxide, arginine; the preservative is at least one of p-hydroxyacetophenone, hexylene glycol, phenoxyethanol, caprylyl glycol.

[0030] The present application has the following beneficial effects:

[0031] 1. The preparation method of the rosemary extract of the present application uses water as the extraction solvent throughout the process, avoiding the use and residue of organic solvents, and the product is safe, the process is more environmentally friendly, the extraction process is low-temperature throughout, the extraction efficiency is high, the process is simple and easy to operate, does not affect the structure and activity of the extract, can maximize the retention of the biological activity of the effective components, and has the advantages of high efficiency, safety, environmental protection, economy, etc., and the prepared rosemary extract has light color, high purity of active ingredients, and is more easily applied in high-end transparent texture cosmetics.

[0032] 2. The cosmetic of the present application, by scientifically compounding the high-activity rosemary extract prepared by the above method with specific barrier repair components (such as squalane, erythritol, ceramide NP) and other natural anti-inflammatory components (such as asiaticoside extract), can produce significant synergistic effect, and the obtained cosmetic has good moisturizing, soothing, repairing and antioxidant effects, and is suitable for sensitive skin care. DETAILED DESCRIPTION

[0033] In order to make the technical problems, technical solutions and technical advantages of the present application clearer, specific examples will be described in detail below, but the protection scope of the present application is not limited to the following specific examples, and the described examples are only a part of the examples of the present application, rather than all the examples, and are not a limitation of the present application. Based on the examples in the present application, all other examples obtained by those skilled in the art without creative labor belong to the scope of protection of the present application.

[0034] Unless otherwise defined, all professional terms used below have the same meaning as generally understood by those skilled in the art. The professional terms used in this paper are only for the purpose of describing specific examples, and are not intended to limit the protection scope of the present application.

[0035] Unless otherwise specifically indicated, all materials, reagents, solvents, and the like used in the present application are available from commercial sources or are prepared by routine methods.

[0036] Examples 1-3 and Comparative Examples 1-3 of the present application each provide a cosmetic, the preparation of which uses the raw materials in the amounts shown in Table 1 below; units are mass percentages.

[0037] Table 1 Formulation of raw materials for preparing examples and comparative examples

[0038]

[0039] The preparation method of the rosemary extract of the present application includes the following steps:

[0040] S1. Pretreatment: dry rosemary leaves are placed in a microwave device and subjected to stabilization treatment at 49°C under microwave conditions for 5 min, and then are pulverized into rosemary powder of 80 mesh using a high-speed shearing pulverizer.

[0041] S2. Soaking and solubilization: the rosemary powder is added to a purified aqueous solution of alkyl polyglycoside APG0810 having a mass concentration of 0.2% at 65°C at a solid-liquid ratio of 1:12 (w / v), and is soaked for 30 min to obtain a mixture;

[0042] S3. Composite enzyme-pulse ultrasonic synergistic extraction: the mixture is cooled to 48°C, and the pH is adjusted to 5.0, and then composite biological enzymes (cellulase: pectinase: lipase = 1:1:0.5, the above ratio is a mass ratio) are added in an amount of 1.5% of the mass of the mixture, and immediately subjected to pulse ultrasonic treatment (power 250W, frequency 40kHz, working cycle 5s on / 5s off) for 35 min, and then rapidly heated to 85°C for 5 min to inactivate the enzymes, to obtain an enzymatic hydrolysate;

[0043] S4. Separation and purification: the enzymatic hydrolysate is centrifuged at a speed of 10000 rpm for 15 min, and the supernatant is subjected to decolorization treatment for 50 min by circulating through a decolorization column filled with activated carbon, to obtain a clear extract;

[0044] S5. Concentration and drying: the clear extract is concentrated by rotary evaporation under the conditions of 62°C and -0.09MPa to a relative density of 1.15, and then vacuum freeze-dried to obtain a light yellow rosemary extract powder.

[0045] The preparation method of the rosemary extract of the present application includes the following steps:

[0046] S1. Pretreatment: dry rosemary leaves are placed in a microwave device and subjected to stabilization treatment at 49°C under microwave conditions for 5 min, and then are pulverized into rosemary powder of 80 mesh using a high-speed shearing pulverizer.

[0047] S2. Infiltration and extraction: rosemary powder was added to purified water at 85°C at a solid-liquid ratio of 1:15 (w / v), and soaked for 40 min to obtain a mixture;

[0048] S3. Hot reflux extraction: the mixture was placed in a hot reflux extraction device, and extracted twice at 90°C, with the first extraction lasting 90 min and the second extraction lasting 60 min, and the two extraction solutions were combined;

[0049] S4. Separation and primary filtration: the combined extraction solution was filtered while hot through a 200-mesh double filter to remove the residue, and a crude extract was obtained;

[0050] S5. Refining and concentration: the crude extract was concentrated by rotary evaporation at 65°C and -0.08 MPa to a relative density of 1.10, and a concentrated solution was obtained;

[0051] S6. Drying: the concentrated solution was spray dried in a spray drying tower with an inlet temperature of 180°C and an outlet temperature of 85°C to obtain a yellow-brown rosemary extract powder.

[0052] The cosmetic preparation method of the above Example 1 is as follows:

[0053] 1. Mix the components of phase A and heat to 78°C, and stir until completely dissolved;

[0054] 2. Mix the components of phase B and heat to 78°C, and stir until uniform and transparent;

[0055] 3. Slowly add phase B to phase A under high-speed homogenization, and homogenize for 5 min;

[0056] 4. Add the components of phase C (sodium hydroxide) to adjust the pH to 6.0, and obtain material 1;

[0057] 5. Dissolve the components of phase D to transparent at 55-60°C, and reserve;

[0058] 6. Cool material 1 to 45°C, and add phases D and E in sequence, stir at low speed for 15 min, and then cool to 30°C and discharge.

[0059] The preparation methods of the cosmetics of Examples 2-3 and Comparative Examples 1-3 are the same, and if there are no added / replaced components, the corresponding preparation steps can be deleted / replaced.

[0060] The cosmetics prepared in Examples 1-3 and Comparative Examples 1-3 above were tested as follows.

[0061] Volunteers were selected to use the cosmetics of the examples and comparative examples and instrument efficacy evaluation was performed, and the volunteers were asked to fill out questionnaires to score the soothing effect and moisturizing effect. In addition, the IL-6 inhibition rate and DPPH clearance rate of the cosmetics of the examples and comparative examples were tested.

[0062] 1. Instrument efficacy evaluation

[0063] Test instrument: The stratum corneum moisture content of the sample area of the subject was tested with a Corneometer CM825 moisture test probe, 2 tests were performed in parallel for each area, and the average value was taken as the stratum corneum moisture content; the transepidermal water loss of the sample area of the subject was tested with a Tewameter TM300 moisture loss probe, 3 tests were performed for each area, and the average value was taken as the transepidermal water loss; the facial image analyzer VisioFace 1000D was used to test the erythema obvious area of the face, and the a* value was recorded.

[0064] Test method: 90 volunteers aged 20-45 years old who met the sensitive skin characteristics were selected and randomly divided into 6 groups, 15 people in each group, corresponding to the cosmetic samples of Examples 1-3 and Comparative Examples 1-3. The volunteers evenly applied the products to the whole face after cleansing in the morning and evening every day, and continuously used them for 28 days.

[0065] This test used a self-control design before and after. The indicators were measured before use (0 days) and after use (28 days). Before testing, the researchers marked the same size test area on the same position of the volunteer's two cheeks and used a surgical pen to mark it to ensure that the positions of the two tests were consistent.

[0066] Three days before each test, the test area should not use any other cosmetics or external medicines. On the test day, the face was cleaned with a unified mild cleansing product, rinsed with clean water, and then dried with a tissue paper. All measurements were carried out in a constant temperature and humidity chamber with a temperature of (21.0±1.0) ℃ and a relative humidity of (50±10) %. The volunteers needed to sit in this environment for at least 30 minutes before starting the test.

[0067] Finally, by comparing the changes in TEWL value, stratum corneum moisture content and erythema index a* value of the same test area before and after using the product, the efficacy of the product was evaluated. The test results were averaged from the results of multiple people.

[0068] 2. IL-6 inhibition rate, DPPH clearance rate test process

[0069] (1) IL-6 inhibition rate test (in vitro cell model method):

[0070] Test method: The lipopolysaccharide (LPS)-induced human immortalized keratinocyte (HaCaT) inflammation model was used for evaluation. Well-grown HaCaT cells were inoculated in a 96-well plate and cultured at 37°C in a 5% CO2 incubator until the cells were completely adherent. The old culture medium was discarded, and fresh culture medium containing 1.0 mg / mL of the test sample (Examples 1-3 and Comparative Examples 1-3) was added to the experimental group. The model control group and the blank control group were replaced with the same volume of culture medium without the sample. After 1 hour of pre-culture, LPS (final concentration 10 ng / mL) was added to the experimental group and the model control group to stimulate the cells to produce an inflammatory response, and the blank control group was added with the same volume of PBS. After 24 hours of continuous culture, the cell supernatant was collected. The concentration of IL-6 in the supernatant was determined according to the instructions of the human IL-6 ELISA kit. The IL-6 inhibition rate (%) was calculated according to the formula: IL-6 inhibition rate = [1-(sample group IL-6 concentration-blank group IL-6 concentration) / (model group IL-6 concentration-blank group IL-6 concentration)] x 100%.

[0071] (2) DPPH free radical scavenging rate test (in vitro chemical analysis method):

[0072] Test method: 10.0 mg of each test sample (Examples 1-3 and Comparative Examples 1-3) was precisely weighed, dissolved with anhydrous ethanol and diluted to 10 mL to prepare a sample stock solution of 1.0 mg / mL. 2.0 mL of the sample stock solution was precisely pipetted and mixed with 2.0 mL of 0.2 mmol / L DPPH-ethanol solution. After vortex mixing, the mixture was allowed to react at room temperature in the dark for 30 minutes. At the same time, a sample blank control group (2.0 mL sample stock solution + 2.0 mL anhydrous ethanol) and a DPPH blank control group (2.0 mL anhydrous ethanol + 2.0 mL DPPH solution) were set up. After the reaction was completed, the absorbance value (A) of each reaction system was measured at 517 nm wavelength with anhydrous ethanol as the zero reference. Each sample was measured in triplicate. The DPPH free radical scavenging rate (%) was calculated according to the formula: DPPH scavenging rate = [1-(A sample-A sample blank) / A blank] x 100%.

[0073] The results shown in Table 2 were finally obtained.

[0074] Table 2 Effect evaluation results of examples and comparative examples

[0075]

[0076] By comparing Examples 1-3 with Comparative Examples 1-3, it can be seen that, compared with after using the cosmetic containing the water extraction rosemary extract or the cosmetic not containing the rosemary extract, after using the cosmetic containing the rosemary extract of the application, the TEWL value is significantly reduced, the stratum corneum water content is significantly increased, the erythema index is significantly reduced, the IL-6 inhibition rate and the DPPH clearance rate are significantly increased, the soothing score and the moisturizing score are both obviously increased, there is a synergistic effect of "1+1>2" between the rosemary extract and the radix notoginseng extract in the surface system, and between the ceramide NP and the barrier repair ingredients such as squalane, and the cosmetic containing the same has good moisturizing, soothing, barrier repair and antioxidant effects; at the same time, it shows that the rosemary extract of the application has a high content of active ingredients, which can prove the superiority of the improved preparation process of the rosemary extract of the application in improving the performance of the product.

[0077] By comparing Examples 1-3, it can be seen that, when the addition amount of the rosemary extract of the application in the cosmetic is 1.5%, the addition amount of the radix notoginseng extract is 1.5%, the addition amount of the ceramide NP is 0.1%, and the addition amount of the erythritol is 4%, the comprehensive effect is the best.

[0078] By comparing Example 2 with Comparative Example 1, it can be seen that, when the rosemary extract and the radix notoginseng extract are added in the cosmetic, the comprehensive effect is the best, which proves that the rosemary extract and the radix notoginseng extract of the application are the core contributors of the efficacy.

[0079] By comparing Example 2 with Comparative Example 3, it can be seen that, when the squalane and the ceramide NP are absent in the cosmetic, the improvement of the barrier repair index is limited, which shows the importance of the barrier repair ingredients in the system.

[0080] The above describes the preferred embodiments of the application, but the application is not limited to the specific details in the above embodiments, and various simple modifications can be made to the technical solutions of the application within the technical concept of the application, and these simple modifications all belong to the protection scope of the application.

[0081] In addition, it should be noted that, in the above specific embodiments, each specific technical feature described can be combined in any appropriate manner without contradiction. In order to avoid unnecessary repetition, the application will not further describe various possible combinations.

[0082] In addition, various different embodiments of the application can also be combined in any manner, as long as they do not deviate from the idea of the application, and they should also be considered as disclosed by the application.

Claims

1. A method for preparing rosemary extract, characterized by, It comprises the following steps: S1. Pretreatment: Take dry rosemary leaves, microwave stabilization treatment under low temperature conditions, and then crush them into rosemary powder with a mesh size of 80-100; S2. Soaking and solubilization: Add rosemary powder into purified water solution containing natural surfactant, soak to obtain a mixture; S3. Composite enzyme-pulse ultrasonic synergistic extraction: cool down and adjust the pH of the mixture, add composite biological enzyme, and immediately treat with pulse ultrasonic, obtain enzyme solution after enzyme inactivation; wherein the composite biological enzyme is cellulase, pectinase and lipase; S4. Separation and purification: centrifuge the enzyme solution, decolorize the supernatant to obtain clear extract; S5. Concentration and drying: concentrate and dry the clear extract by rotary evaporation to obtain rosemary extract powder.

2. The production method according to claim 1, characterized by, In the step S1, the low temperature is 48-50℃; the microwave stabilization treatment time is 5min; and the crushing is performed by a high-speed shearing crusher.

3. The preparation method according to claim 1, characterized in that, In the step S2, the mass ratio of rosemary powder to purified water solution containing natural surfactant is 1:10-15; the temperature of purified water solution containing natural surfactant is 60-70℃, wherein the mass concentration of natural surfactant is 0.1-0.3%; and the soaking time is 30-40min.

4. The method of claim 1, wherein, In the step S3, the temperature is cooled down to 45-50℃, and the pH is adjusted to 4.5-5.5; the addition amount of composite biological enzyme is 0.3-3.0% of the mass of the mixture; the mass ratio of cellulase, pectinase and lipase in the composite biological enzyme is (0.8-1.2):(0.8-1.2):(0.4-0.6); in the step S3, the parameters of pulse ultrasonic treatment are: power 200-300W, frequency 40kHz, working cycle 5s on / 5s off, and total treatment time 30-40min; and the enzyme inactivation is to rapidly heat the mixture to 85-90℃ for 5min after enzyme hydrolysis, and then cool down to 50-65℃.

5. The preparation method according to claim 1, characterized in that, In the step S4, the centrifugation is centrifugal separation at a speed of 8000-10000rpm for 15min, and the decolorization is cyclic decolorization of the supernatant through a decolorization column filled with activated carbon for 40-60min.

6. The method of claim 1, wherein, In the step S5, the rotary concentration is rotary evaporation concentration at 60-65℃ under reduced pressure of -0.08--0.1MPa to obtain extract with a relative density of 1.10-1.20; and the drying is vacuum freeze drying or spray drying with an inlet temperature ≤65℃.

7. Rosemary extract prepared by the preparation method of any one of claims 1-6.

8. A cosmetic product, characterized by, A rosemary extract as claimed in claim 7.

9. The cosmetic product according to claim 8, characterized in that, The cosmetic comprises the following mass percentage of components: the rosemary extract 2.0-4.0%, the centella asiatica extract 1.0-2.0%; erythritol 3.0-5.0%, ceramide NP 0.05-0.2%, squalane 3.0-5.0%; humectant 3-8%, emulsifier 1-4%, thickening stabilizer 0.1-0.5%, emollient 1-5%, pH regulator 0.001-1%, preservative 0.5-1.5%, chelating agent 0.02-0.1%, and the balance is deionized water.

10. The cosmetic product according to claim 9, characterized in that, The humectant is at least one of glycerin, butylene glycol, panthenol, and sugar isomer; the emulsifier is at least one of hydrogenated lecithin, cetearyl glucoside, PEG-100 stearate, cetearyl olivate, and sorbitan olivate; the thickening stabilizer is at least one of carbomer, xanthan gum, acryloyldimethyltaurine ammonium / VP copolymer, and magnesium aluminum silicate; the emollient is at least one of dimethicone, caprylic / capric triglyceride, C15-19 alkane, and isononyl isononanoate; the pH regulator is at least one of triethanolamine, sodium hydroxide, and arginine; the preservative is at least one of p-hydroxyacetophenone, hexylene glycol, phenoxyethanol, and caprylyl glycol; and the chelating agent is at least one of disodium ethylenediaminetetraacetate and tetrasodium ethylenediaminetetraacetate.

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