Oral care composition for enhancing anti-allergy effect and application thereof

Abstract

The invention relates to the technical field of toothpaste raw materials, and particularly discloses an oral care composition for enhancing an anti-allergy effect and application thereof. The oral care composition capable of enhancing the anti-allergy effect comprises the following components in parts by mass: 0.9-3 parts of a composite plant extract; 0.5 to 2 parts of an anti-allergy agent; 0.1 to 0.3 part of an anti-caries agent; 0.01 to 0.03 part of a stabilizing agent; 0.08 to 0.12 part of a pH (Potential of Hydrogen) regulator; the composite plant extract is prepared by compounding a liquorice extract, a gastrodia elata extract and an agilawood extract; the stabilizer is rosmarinic acid. The anti-allergy toothpaste has the advantages that the anti-allergy effect of the toothpaste product is remarkable, harmful bacteria in the oral cavity can be inhibited, and gingival problems are relieved.

Description

Technical Field

[0001] This invention relates to the field of toothpaste raw materials, and in particular to an oral care composition that enhances anti-sensitivity effects and its application. Background Technology

[0002] Tooth sensitivity is a common symptom in oral health. When teeth are stimulated by hot, cold, sweet, sour, or mechanical forces, they will experience pain and irritation. The mainstream theory is that this is essentially due to the exposure of dentin, which stimulates the nerves in the dentinal tubules.

[0003] Common methods used in commercially available desensitizing toothpastes include: Potassium nitrate: Potassium ions can act on dental nerve fibers, reduce nerve excitability, block the transmission of pain signals, and thus reduce the sensation of pain; Strontium chloride: Strontium ions can combine with carbonate ions, phosphate ions, etc. in saliva to form insoluble strontium salts that precipitate in the dentinal tubules, thereby sealing the dentinal tubules and relieving tooth sensitivity.

[0004] Sodium fluoride: Sodium fluoride can react with hydroxyapatite on the tooth surface to form fluorapatite. Fluorapatite is less soluble in acid than hydroxyapatite, which can enhance the acid resistance of teeth. At the same time, it can also promote the remineralization of dentin, repair minor damage to the tooth surface, make teeth stronger, and reduce the impact of external stimuli on the dental pulp. Arginine: It can combine with minerals on the tooth surface to form a protective film. It can also regulate the acid-base balance in the oral cavity, inhibit the erosion of teeth by acidic substances, reduce the stimulation of dentin by acid, and thus relieve tooth sensitivity.

[0005] Bioactive glass: When it comes into contact with saliva in the mouth, it releases ions such as calcium and phosphorus. These ions can interact with the minerals on the tooth surface, promote tooth remineralization, repair damaged dentin, and fill dentinal tubules, thereby achieving the purpose of sealing dentinal tubules and blocking irritants.

[0006] While commercially available desensitizing toothpastes can relieve sensitivity symptoms, they have drawbacks such as limited ingredients, masking underlying diseases, and the risk of long-term dependence. For example, potassium nitrate only numbs nerves and is ineffective against root causes such as gingival recession, enamel abrasion, and exposed tooth roots; the recurrence rate of sensitivity after discontinuation is >70%. Strontium chloride sealing layers are easily worn away by acid / brushing. Bioactive glass relies on saliva for activation, is ineffective for those with dry mouth, and has an effectiveness rate of <30% for people with low saliva flow. Therefore, there is still room for improvement.

[0007] It is necessary to develop an anti-allergy product that can inhibit harmful bacteria in the mouth, alleviate gum problems, and has a significant anti-allergy effect. Summary of the Invention

[0008] In order to enable toothpaste products to have significant anti-sensitivity effects while inhibiting harmful bacteria in the oral cavity and alleviating gum problems, this application provides an oral care composition with enhanced anti-sensitivity effects and its application.

[0009] In a first aspect, this application provides an oral care composition with enhanced anti-allergy effects, employing the following technical solution: An oral care composition with enhanced anti-sensitivity effects comprises the following components in parts by weight: Compound plant extract 0.9-3 parts; 0.5-2 parts of anti-allergy agent; 0.1-0.3 parts of anti-caries agent; Stabilizer 0.01-0.03 parts; pH adjuster 0.08-0.12 parts; The compound plant extract is a combination of licorice extract, gastrodia extract, and agarwood extract; The stabilizer is rosmarinic acid.

[0010] By adopting the above technical solution, a compound plant extract is prepared by combining licorice extract, gastrodia extract, and agarwood extract. The main components of licorice extract include triterpenoids (such as glycyrrhizic acid and glycyrrhetinic acid), flavonoids (such as glycyrrhizin and isoglycyrrhizin), polysaccharides (such as glycyrrhizin), coumarins (such as glycyrrhizin), and other components (such as amino acids and minerals). The main components of gastrodia extract include gastrodin, gastrogenin, gastrodin ether, pelicin, vanillin, β2-sterol, p-hydroxybenzaldehyde, citric acid, succinic acid, etc. The main components of agarwood extract are volatile oils (sesquiterpenes such as agarinuron, agarinspirol, guaiacol, aromatic compounds, etc.), chromones, and fatty acids, etc.

[0011] Through the synergistic effect of the above active ingredients: It can block the TRPV1 pain channel (pain receptor) and activate GABA receptors, reduce the excitability of dental pulp nerves, alleviate soreness caused by hot and cold stimuli, and achieve the effect of reducing nerve sensitivity. It can destroy bacterial biofilms and has a very good inhibitory effect on cariogenic bacteria such as Streptococcus mutans, Porphyromonas gingivalis, and Candida albicans (fungus); It can inhibit inflammatory pathways, block NF-κB signaling, downregulate IL-6 and TNF-α (key factors in gingival inflammation), and inhibit the COX-2 / PGE2 pathway, thereby reducing gingival redness, swelling, and pain. It can enhance the expression of epidermal growth factor (EGF), increase the proliferation rate of gingival fibroblasts, accelerate the regeneration of oral ulcer epithelium, and increase the secretion of mucin MUC5AC to form a physical protective film on the wound surface with the help of resinous substances, thereby reducing food irritation and pain. It can also stimulate salivary gland secretion and relieve dry mouth.

[0012] The above components, combined with desensitizing agents and anti-caries agents, form a remineralized protective layer on the tooth surface, effectively relieving sensitivity problems. It can comprehensively alleviate various oral problems, making the anti-sensitivity effect better and longer-lasting. It achieves the technical effect of making the toothpaste product significantly effective in relieving sensitivity while inhibiting harmful bacteria in the mouth and reducing gum problems.

[0013] By adding rosmarinic acid, the photothermal decomposition of the active ingredients is greatly reduced, resulting in more stable product quality and the ability to exert antibacterial, anti-allergic, and gum-repairing effects over a long period of time.

[0014] Preferably, the mass ratio of licorice extract, gastrodia extract, and agarwood extract in the compound plant extract is 0.3-1:0.3-1:0.3-1.

[0015] By adopting the above technical solution, and by combining licorice extract, gastrodia extract, and agarwood extract in a special ratio, the effect of alleviating gum problems is more significant.

[0016] Preferably, the anti-allergy agent is strontium chloride, the anti-caries agent is sodium fluoride, and the pH adjuster is a mixture of citric acid and sodium citrate.

[0017] By adopting the above technical solutions, the remineralization protective layer is formed more effectively, thus mitigating the sensitive issues more effectively.

[0018] Preferably, in the pH adjuster, the mass ratio of citric acid to sodium citrate is 0.04-0.06:0.04-0.06.

[0019] By adopting the above technical solution, citric acid and sodium citrate are combined in a specific ratio to adjust the pH value of the formula, so that the pH value of the formula is stabilized at 6-7.5. This makes it less likely for the active ingredients in the effective compound plant extracts to become inactive, better maintain their activity, and make the product quality more stable.

[0020] Secondly, this application provides an application of an oral care composition that enhances anti-allergy effects, employing the following technical solution: Application of the above-mentioned enhanced desensitizing oral care composition, wherein the enhanced desensitizing oral care composition is used to prepare toothpaste, the toothpaste comprising the following components in parts by weight: Moisturizer 62.3-63.7 parts; 0.8-1.2 parts adhesive; 19.5-20.5 parts of friction agent; 1.9-2.1 parts of foaming agent; Taste improver 0.15-0.25 parts; Preservative: 0.15-0.25 parts; Fragrance agent 0.9-1.1 parts; Enhanced anti-allergy oral care composition: 1.59-5.45 parts; Solvent: 5.45-12.71 parts.

[0021] By adopting the above technical solutions, the toothpaste not only has good cleaning ability, but also has long-lasting antibacterial effect after brushing, relieves tooth sensitivity, promotes gum repair, and improves gum problems.

[0022] Preferably, the moisturizer is a compound of sorbitol, glycerin and propylene glycol, wherein the mass ratio of sorbitol, glycerin and propylene glycol is 56.5-57.5:2.9-3.1:2.9-3.1.

[0023] By adopting the above technical solutions, the oral sensation after brushing can be improved, dryness in the mouth can be reduced, and the user experience can be enhanced.

[0024] Preferably, the adhesive is a compound of cellulose gum, xanthan gum, and carrageenan extract, wherein the mass ratio of cellulose gum, xanthan gum, and carrageenan extract is 0.5-0.7:0.15-0.25:0.15-0.25.

[0025] By adopting the above technical solutions, the quality of the toothpaste is relatively stable and it is more convenient to use.

[0026] Preferably, the abrasive is hydrated silica, the foaming agent is sodium lauryl sulfate, the taste modifier is sodium saccharin, the preservative is sodium benzoate, the fragrance is flavoring, and the solvent is water.

[0027] By adopting the above technical solutions, the toothpaste has better cleaning ability and a better user experience.

[0028] In summary, this application has the following beneficial effects: 1. Because this application uses a compound of plant extracts, namely licorice extract, gastrodia extract, and agarwood extract, combined with anti-sensitivity agents and anti-caries agents, to form a remineralized protective layer on the tooth surface, it effectively relieves sensitivity problems; it can comprehensively relieve various oral problems, making the anti-sensitivity effect better and longer-lasting, and achieving the technical effect of making the toothpaste product significantly effective in relieving sensitivity while inhibiting harmful bacteria in the oral cavity and reducing gum problems.

[0029] 2. In this application, licorice extract, gastrodia extract, and agarwood extract are preferably combined in a special ratio to make the effect of alleviating gum problems more significant.

[0030] 3. In this application, the addition of rosmarinic acid significantly reduces the photothermal decomposition of the active ingredients, resulting in more stable product quality and enabling it to exert its antibacterial, anti-allergic, and gum-repairing effects over a long period. Detailed Implementation

[0031] The present application will be further described in detail below with reference to the embodiments.

[0032] Example 1 An oral care composition with enhanced anti-sensitivity effects comprises the following components: Compound plant extracts, anti-allergens, anti-caries agents, stabilizers, and pH adjusters.

[0033] The compound plant extract is a combination of licorice extract, gastrodia extract, and agarwood extract.

[0034] The licorice extract was purchased from Xi'an An'ao Biotechnology Co., Ltd., and is a water-soluble extract.

[0035] Gastrodia elata extract was purchased from Xi'an An'ao Biotechnology Co., Ltd., and is a water-soluble extract.

[0036] The agarwood extract was purchased from Shaanxi Tianze Qiannuo Biotechnology Co., Ltd., and is a water-soluble extract.

[0037] The stabilizer was rosmarinic acid, which was purchased from Shaanxi Tianze Qiannuo Biotechnology Co., Ltd.

[0038] The anti-allergen is strontium chloride, purchased from Guangzhou Weisikang Chemical Products Co., Ltd.

[0039] The caries inhibitor is sodium fluoride, purchased from Guangzhou Naying Chemical Co., Ltd.

[0040] The pH adjuster is a mixture of citric acid and sodium citrate.

[0041] Citric acid was purchased from Guangzhou Weisikang Chemical Products Co., Ltd.

[0042] Sodium citrate was purchased from Guangzhou Weisikang Chemical Products Co., Ltd.

[0043] Preparation method of oral care composition with enhanced anti-allergic effect: Mix 0.3 kg of licorice extract, 0.3 kg of gastrodia extract, 0.3 kg of agarwood extract, 0.5 kg of strontium chloride, 0.1 kg of sodium fluoride, 0.01 kg of rosmarinic acid, 0.04 kg of citric acid, and 0.04 kg of sodium citrate evenly to obtain an oral care composition with enhanced anti-allergic effect.

[0044] Example 2 An oral care composition with enhanced anti-sensitivity effects comprises the following components: Compound plant extracts, anti-allergens, anti-caries agents, stabilizers, and pH adjusters.

[0045] The compound plant extract is a combination of licorice extract, gastrodia extract, and agarwood extract.

[0046] The licorice extract was purchased from Xi'an An'ao Biotechnology Co., Ltd., and is a water-soluble extract.

[0047] Gastrodia elata extract was purchased from Xi'an An'ao Biotechnology Co., Ltd., and is a water-soluble extract.

[0048] The agarwood extract was purchased from Shaanxi Tianze Qiannuo Biotechnology Co., Ltd., and is a water-soluble extract.

[0049] The stabilizer was rosmarinic acid, which was purchased from Shaanxi Tianze Qiannuo Biotechnology Co., Ltd.

[0050] The anti-allergen is strontium chloride, purchased from Guangzhou Weisikang Chemical Products Co., Ltd.

[0051] The caries inhibitor is sodium fluoride, purchased from Guangzhou Naying Chemical Co., Ltd.

[0052] The pH adjuster is a mixture of citric acid and sodium citrate.

[0053] Citric acid was purchased from Guangzhou Weisikang Chemical Products Co., Ltd.

[0054] Sodium citrate was purchased from Guangzhou Weisikang Chemical Products Co., Ltd.

[0055] Preparation method of oral care composition with enhanced anti-allergic effect: Mix 0.65 kg of licorice extract, 0.65 kg of gastrodia extract, 0.65 kg of agarwood extract, 1 kg of strontium chloride, 0.2 kg of sodium fluoride, 0.02 kg of rosmarinic acid, 0.05 kg of citric acid, and 0.05 kg of sodium citrate evenly to obtain an oral care composition with enhanced anti-allergic effect.

[0056] Example 3 An oral care composition with enhanced anti-sensitivity effects comprises the following components: Compound plant extracts, anti-allergens, anti-caries agents, stabilizers, and pH adjusters.

[0057] The compound plant extract is a combination of licorice extract, gastrodia extract, and agarwood extract.

[0058] The licorice extract was purchased from Xi'an An'ao Biotechnology Co., Ltd., and is a water-soluble extract.

[0059] Gastrodia elata extract was purchased from Xi'an An'ao Biotechnology Co., Ltd., and is a water-soluble extract.

[0060] The agarwood extract was purchased from Shaanxi Tianze Qiannuo Biotechnology Co., Ltd., and is a water-soluble extract.

[0061] The stabilizer was rosmarinic acid, which was purchased from Shaanxi Tianze Qiannuo Biotechnology Co., Ltd.

[0062] The anti-allergen is strontium chloride, purchased from Guangzhou Weisikang Chemical Products Co., Ltd.

[0063] The caries inhibitor is sodium fluoride, purchased from Guangzhou Naying Chemical Co., Ltd.

[0064] The pH adjuster is a mixture of citric acid and sodium citrate.

[0065] Citric acid was purchased from Guangzhou Weisikang Chemical Products Co., Ltd.

[0066] Sodium citrate was purchased from Guangzhou Weisikang Chemical Products Co., Ltd.

[0067] Preparation method of oral care composition with enhanced anti-allergic effect: Mix 1 kg of licorice extract, 1 kg of gastrodia extract, 1 kg of agarwood extract, 2 kg of strontium chloride, 0.3 kg of sodium fluoride, 0.03 kg of rosmarinic acid, 0.06 kg of citric acid, and 0.06 kg of sodium citrate evenly to obtain an oral care composition with enhanced anti-allergic effect.

[0068] Comparative Example 1 An oral care composition with enhanced anti-allergy effects, differing from Example 2 only in that: The licorice extract was replaced with an equal amount of Platycodon grandiflorus extract.

[0069] The Platycodon grandiflorus extract was purchased from Shanyang Lianfeng Biotechnology Co., Ltd.

[0070] Comparative Example 2 An oral care composition with enhanced anti-allergy effects, differing from Example 2 only in that: The extract of Gastrodia elata was replaced with an equal amount of extract of Ophiopogon japonicus.

[0071] The extract of Ophiopogon japonicus was purchased from Ningxia Xiangcao Biotechnology Co., Ltd.

[0072] Comparative Example 3 An oral care composition with enhanced anti-allergy effects, differing from Example 2 only in that: Hops extract was used to replace agarwood extract in equal amounts.

[0073] The hop extract was purchased from Shanyang Lianfeng Biotechnology Co., Ltd.

[0074] Comparative Example 4 An oral care composition with enhanced anti-allergy effects, differing from Example 2 only in that: Platycodon grandiflorus extract was used to replace licorice extract in equal amounts; Gastrodia elata extract was used to replace gastrodia elata extract in equal amounts; and Aquilaria sinensis extract was used to replace hops extract in equal amounts.

[0075] The Platycodon grandiflorus extract was purchased from Shanyang Lianfeng Biotechnology Co., Ltd.

[0076] The extract of Ophiopogon japonicus was purchased from Ningxia Xiangcao Biotechnology Co., Ltd.

[0077] The hop extract was purchased from Shanyang Lianfeng Biotechnology Co., Ltd.

[0078] Application Example 1 A toothpaste, the preparation method of which is as follows: Step 1): Add 12.71 kg of water to the mixing tank, start the vacuum pump valve, and when the vacuum degree reaches ≥-0.06 MPa, open the feed valve and add 0.15 kg of sodium saccharin and 0.15 kg of sodium benzoate into the water. Let it stand for 5 minutes to obtain the premix.

[0079] Step 2): Add 56.5 kg of sorbitol, 2.9 kg of glycerol and 2.9 kg of propylene glycol to the premix, let stand for 5 min to obtain the mixture.

[0080] Step 3): Continue evacuating until the vacuum level rises to ≥-0.08 MPa. Start stirring with a scraper at 20 r / min. Add 0.5 kg cellulose gum, 0.15 kg xanthan gum, 0.15 kg Carrageenan extract, 1.9 kg sodium lauryl sulfate, and 19.5 kg hydrated silica to the mixture. Maintain the stirring speed at 20 r / min and stir for 30 minutes to obtain the pre-made paste.

[0081] Step 4) Add 0.9 kg of fragrance and 1.59 kg of oral care composition with enhanced anti-allergy effect to the pre-made paste, keep the speed at 20 r / min and stir for 15 minutes to obtain the mixed paste.

[0082] Step 5): Vacuum the mixture until it is uniform and smooth, then discharge the mixture to obtain toothpaste.

[0083] The oral care composition with enhanced anti-allergy effect is the oral care composition with enhanced anti-allergy effect of Example 1.

[0084] Sorbitol was purchased from Zhaoqing Huanfa Biotechnology Co., Ltd.

[0085] Glycerin was purchased from Qixiao International Trade (Guangzhou) Co., Ltd.

[0086] Propylene glycol was purchased from Qixiao International Trade (Guangzhou) Co., Ltd.

[0087] The cellulose gum was purchased from Changshu Weiyi Technology Co., Ltd.

[0088] Xanthan gum was purchased from Guangzhou Naying Chemical Co., Ltd.

[0089] The extract of *Chlorophytum comosum* was purchased from Guangzhou Jiahui Chemical Co., Ltd.

[0090] Hydrated silica was purchased from Jin Sanjiang (Zhaoqing) Silicon Materials Co., Ltd.

[0091] Sodium lauryl sulfate was purchased from Guangzhou Helong Trading Co., Ltd.

[0092] Sodium saccharin was purchased from Guangzhou Juhui Flavoring Co., Ltd.

[0093] Sodium benzoate was purchased from Guangzhou Weisikang Chemical Products Co., Ltd.

[0094] The fragrance was purchased from Guangzhou Tufu Fragrance Technology Co., Ltd.

[0095] Application Example 2 A toothpaste, the preparation method of which is as follows: Step 1): Add 9.33 kg of water to the mixing tank, start the vacuum pump valve, and when the vacuum degree reaches ≥-0.06 MPa, open the feed valve and add 0.2 kg of sodium saccharin and 0.2 kg of sodium benzoate to the water. Let it stand for 5 minutes to obtain the premix.

[0096] Step 2): Add 57 kg of sorbitol, 3 kg of glycerol, and 3 kg of propylene glycol to the premix, let stand for 5 minutes, and obtain the mixture.

[0097] Step 3): Continue evacuating the vacuum until the vacuum level rises to ≥-0.08 MPa. Start stirring with a scraper at 20 r / min. Add 0.6 kg of cellulose gum, 0.2 kg of xanthan gum, 0.2 kg of Carrageenan extract, 2 kg of sodium lauryl sulfate, and 20 kg of hydrated silica to the mixture. Maintain the stirring speed at 20 r / min and stir for 30 minutes to obtain the pre-made paste.

[0098] Step 4) Add 1 kg of fragrance and 3.27 kg of oral care composition with enhanced anti-allergy effect to the pre-made paste, keep the speed at 20 r / min and stir for 15 minutes to obtain the mixed paste.

[0099] Step 5): Vacuum the mixture until it is uniform and smooth, then discharge the mixture to obtain toothpaste.

[0100] The enhanced anti-allergy oral care composition is the enhanced anti-allergy oral care composition of Example 2.

[0101] Sorbitol was purchased from Zhaoqing Huanfa Biotechnology Co., Ltd.

[0102] Glycerin was purchased from Qixiao International Trade (Guangzhou) Co., Ltd.

[0103] Propylene glycol was purchased from Qixiao International Trade (Guangzhou) Co., Ltd.

[0104] The cellulose gum was purchased from Changshu Weiyi Technology Co., Ltd.

[0105] Xanthan gum was purchased from Guangzhou Naying Chemical Co., Ltd.

[0106] The extract of *Chlorophytum comosum* was purchased from Guangzhou Jiahui Chemical Co., Ltd.

[0107] Hydrated silica was purchased from Jin Sanjiang (Zhaoqing) Silicon Materials Co., Ltd.

[0108] Sodium lauryl sulfate was purchased from Guangzhou Helong Trading Co., Ltd.

[0109] Sodium saccharin was purchased from Guangzhou Juhui Flavoring Co., Ltd.

[0110] Sodium benzoate was purchased from Guangzhou Weisikang Chemical Products Co., Ltd.

[0111] The fragrance was purchased from Guangzhou Tufu Fragrance Technology Co., Ltd.

[0112] Application Example 3 A toothpaste, the preparation method of which is as follows: Step 1): Add 5.45 kg of water to the mixing tank, start the vacuum pump valve, and when the vacuum degree reaches ≥-0.06 MPa, open the feed valve and add 0.25 kg of sodium saccharin and 0.25 kg of sodium benzoate into the water. Let it stand for 5 minutes to obtain the premix.

[0113] Step 2): Add 57.5 kg of sorbitol, 3.1 kg of glycerol and 3.1 kg of propylene glycol to the premix, let stand for 5 min to obtain the mixture.

[0114] Step 3): Continue evacuating until the vacuum level rises to ≥-0.08 MPa. Start stirring with a scraper at 20 r / min. Add 0.7 kg cellulose gum, 0.25 kg xanthan gum, 0.25 kg Carrageenan extract, 2.1 kg sodium lauryl sulfate, and 20.5 kg hydrated silica to the mixture. Maintain the stirring speed at 20 r / min and stir for 30 minutes to obtain the pre-prepared paste.

[0115] Step 4) Add 1.1 kg of fragrance and 5.45 kg of oral care composition with enhanced anti-allergy effect to the pre-made paste, keep the speed at 20 r / min and stir for 15 minutes to obtain the mixed paste.

[0116] Step 5): Vacuum the mixture until it is uniform and smooth, then discharge the mixture to obtain toothpaste.

[0117] The oral care composition with enhanced anti-allergy effect is the oral care composition with enhanced anti-allergy effect described in Example 3.

[0118] Sorbitol was purchased from Zhaoqing Huanfa Biotechnology Co., Ltd.

[0119] Glycerin was purchased from Qixiao International Trade (Guangzhou) Co., Ltd.

[0120] Propylene glycol was purchased from Qixiao International Trade (Guangzhou) Co., Ltd.

[0121] The cellulose gum was purchased from Changshu Weiyi Technology Co., Ltd.

[0122] Xanthan gum was purchased from Guangzhou Naying Chemical Co., Ltd.

[0123] The extract of *Chlorophytum comosum* was purchased from Guangzhou Jiahui Chemical Co., Ltd.

[0124] Hydrated silica was purchased from Jin Sanjiang (Zhaoqing) Silicon Materials Co., Ltd.

[0125] Sodium lauryl sulfate was purchased from Guangzhou Helong Trading Co., Ltd.

[0126] Sodium saccharin was purchased from Guangzhou Juhui Flavoring Co., Ltd.

[0127] Sodium benzoate was purchased from Guangzhou Weisikang Chemical Products Co., Ltd.

[0128] The fragrance was purchased from Guangzhou Tufu Fragrance Technology Co., Ltd.

[0129] Comparative Application Example 1 One type of toothpaste, compared to application example 2, differs only in that: The oral care composition with enhanced anti-allergy effect is the oral care composition with enhanced anti-allergy effect of Comparative Example 1.

[0130] Comparative Application Example 2 One type of toothpaste, compared to application example 2, differs only in that: The oral care composition with enhanced anti-sensitivity effect is the oral care composition with enhanced anti-sensitivity effect of Comparative Example 2.

[0131] Comparative Application Example 3 One type of toothpaste, compared to application example 2, differs only in that: The oral care composition with enhanced anti-sensitivity effect is the oral care composition with enhanced anti-sensitivity effect of Comparative Example 3.

[0132] Comparative Application Example 4 One type of toothpaste, compared to application example 2, differs only in that: The oral care composition with enhanced anti-sensitivity effect is the oral care composition with enhanced anti-sensitivity effect of Comparative Example 4.

[0133] Experiment 1 According to the test method in section 7.3 of QB / T 2738-2023 "Evaluation Method for Antibacterial and Bacteriostatic Effects of Daily Chemical Products", the antibacterial performance of toothpastes in various application examples and comparative application examples was tested.

[0134] Antibacterial rate (%) = (A - B) / A * 100 In the formula: A—The average colony count of the control sample; B—The average number of colonies in the toothpaste sample.

[0135] Experimental bacterial strain: Staphylococcus aureus.

[0136] The inhibition time was 5 minutes.

[0137] Detailed experimental data are shown in Table 1.

[0138] Experiment 2 Cell scratch assay Principles and methods Test Principles The cell scratch assay is a laboratory technique that studies cell migration, repair capabilities, and intercellular interactions by scratching a cell monolayer and periodically capturing images using a time-lapse microscope. The basic principle is that a blank area called a scratch is artificially created on a fused monolayer of cells. Cells at the edge of the scratch gradually enter the blank area, causing the scratch to heal. Images are captured at the beginning and at regular intervals during cell migration. By measuring the scratch spacing (or area) at different time points and calculating the difference, the cell's migration ability can be assessed.

[0139] Main instruments and reagents Main instruments and equipment: Yamato / HQIP610 CO2 incubator; BSC-1300IIA2 Biosafety Cabinet; HQ / HQICX inverted fluorescence microscope; DL-6000B low-speed refrigerated centrifuge; SYNERGY-H1 Full Wavelength Multifunctional Microplate Reader.

[0140] Main reagents and consumables: HGFs cells (human gingival fibroblasts); DMEM high-glucose medium; fetal bovine serum; Penicillin-streptomycin mixed solution (100× penicillin-streptomycin); thiazolyl blue (MTT).

[0141] Test substance: Blank control: basal culture medium (cell viability assay); incomplete culture medium (scratch assay); positive control: complete culture medium; Sample: Dilute the sample with deionized water to a certain concentration to serve as the sample stock solution, then filter it through a 0.22μm filter, and then dilute it to a series of concentrations.

[0142] Test methods Cell culture: Take the frozen cell culture and inoculate it into the conventional culture medium at an appropriate density for the experiment.

[0143] Cell viability assay: HGFs cells were seeded into 96-well plates. After 24 hours, the culture medium was discarded, and basal culture medium containing different concentrations of the test samples was added. After 24 hours, OD490 was measured by MTT assay, and the effect of the test samples on HGFs cell activity was calculated and analyzed.

[0144] Calculations showed that cell viability was high when the sample concentration was 0.0032%. Therefore, a sample with a concentration of 0.0032% was used for the cell scratch test.

[0145] HGFs cell scratch assay: After culturing HGFs cells in 6-well plates for 24 hours, a streak was made in the center of each well using a sterile pipette tip, and the culture medium was discarded. A blank control group, a positive control group, and a 0.0032% sample group were added, and cell healing was observed at 0 hours and 24 hours.

[0146] analyze Calculation formula: Cell survival rate after drug intervention at different concentrations Survival rate (%) = Sample OD value / Negative control OD value × 100%.

[0147] scratch healing rate 24-hour scratch healing rate (%) = (Scratch area at 1-24 hours / Scratch area at 0 hours) × 100% Detailed experimental data are shown in Table 1.

[0148] Experiment 3 Anti-inflammatory experiment Principles and methods 1. Testing Principle Periodontal tissue is mainly composed of cells and extracellular matrix, and human gingival fibroblasts (HGFs) are an important component of periodontal tissue. HGFs play a crucial role in the formation, regeneration, functional implementation, and repair of periodontal tissue. Lipopolysaccharide (LPS) is one of the main virulence factors of periodontal pathogens, capable of inducing different types of cells to produce large amounts of inflammatory mediators and cytokines, leading to periodontal tissue damage. This experiment investigated the regulatory effect of samples on LPS-induced inflammatory responses in HGFs via the IL-6 signaling pathway, serving as a supporting factor for products aimed at alleviating gum problems.

[0149] 2. Main Instruments, Equipment and Reagents 2.1 Main instruments and equipment: Yamato / HQIP610 CO2 incubator; BSC-1300IIA2 Biosafety Cabinet; HQ / HQICX inverted fluorescence microscope; DL-6000B low-speed refrigerated centrifuge; SYNERGY-H1 Full Wavelength Multifunctional Microplate Reader.

[0150] 2.2 Main reagents and consumables: Human gingival fibroblasts (HGFs cells); DMEM high-glucose medium; Fetal bovine serum; Trypsin solution; Penicillin-streptomycin mixed solution (100× penicillin-streptomycin); MTT (thiazolyl blue); Bacterial lipopolysaccharide (LPS); Human interleukin-6 (IL-6) ELISA kit.

[0151] 2.3 Test substance: Sample: Dilute the sample to a certain concentration to use as the sample stock solution, then filter it through a 0.22μm filter, and then dilute it to a series of concentrations after filtration; Negative controls: basal culture medium (cell viability assay); basal culture medium containing LPS (IL-6 inflammatory factor content assay).

[0152] 3. Testing Methods 3.1 Cell Culture: Take the frozen cell culture and inoculate it into the conventional culture medium at an appropriate density for the experiment.

[0153] 3.2 Cell viability assay: Human gingival fibroblasts were seeded into 96-well plates. After 24 hours, the culture medium was discarded, and basal culture medium containing different concentrations of the test samples was added. After 24 hours, the OD490 was measured by MTT assay, and the effect of the test samples on the activity of human gingival fibroblasts was calculated and analyzed.

[0154] 3.3 Measurement of IL-6 inflammatory factor levels: Human gingival fibroblasts were seeded into 96-well plates and cultured for 24 hours. The culture medium was then replaced with basal medium containing different concentrations of the experimental samples. After 24 hours, the cell supernatant was collected and centrifuged. The supernatant was then aspirated, and the IL-6 content in the cell culture supernatant was measured using an ELISA kit to determine the effect of the experimental samples on the IL-6 content of human gingival fibroblasts.

[0155] 4. Analysis 4.1 Calculation formula: 4.1.1 Cell survival rate after intervention with different drug concentrations: Survival rate = OD sample / OD negative control * 100%.

[0156] 4.1.2 Relative content of IL-6: IL-6 relative content (%) = average IL-6 content of samples / average IL-6 content of negative controls * 100%.

[0157] 4.2 Data Analysis: Statistical analysis using software showed that, at the selected concentration of the test substance, a two-tailed test was used to compare the sample group and the negative control group, with a significance level of α = 0.05. P > 0.05 indicated no significant difference between the two groups; P < 0.05 indicated a significant difference between the two groups.

[0158] 5. Results Analysis ① When the cell viability of the sample at the corresponding concentration is ≥90%, and there is no significant difference in morphology between the sample group cells and the negative group cells, it indicates that the sample has no significant cytotoxicity at that concentration, and this concentration is selected for the experiment.

[0159] ② Compared with the negative control, the test group showed a significant decrease in the IL-6 inflammatory factor content in cells (P<0.05), indicating that the test substance has the ability to inhibit the IL-6 inflammatory factor content in cells at the test concentration. This suggests that the sample can alleviate gingival problems to a certain extent by inhibiting the IL-6 inflammatory factor content.

[0160] According to the survival rate formula, the survival rate is ≥90% when the sample concentration is 0.0032%. Therefore, a sample with a concentration of 0.0032% was selected for the IL-6 relative content (%) experiment.

[0161] Detailed experimental data are shown in Table 1.

[0162] Based on the data comparison of the application examples and comparative application examples in Table 1, it can be seen that all application examples and comparative application examples have good antibacterial properties. Furthermore, the repair and anti-inflammatory effects of the application examples are superior to those of the comparative application examples. This indicates that the composite plant extracts (licorice extract, gastrodia extract, and agarwood extract) in the application examples not only provide excellent anti-sensitivity, antibacterial, and anti-inflammatory effects, but also effectively promote gum repair, providing a more comprehensive solution to oral problems and promoting oral health. In contrast, while the comparative application examples, which used other extracts to replace licorice extract, gastrodia extract, and agarwood extract individually or entirely, also showed good antibacterial and anti-inflammatory effects, the 24-hour scratch healing rate was lower, making it difficult to significantly promote gum repair.

[0163] This specific embodiment is merely an explanation of this application and is not intended to limit it. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but such modifications are protected by patent law as long as they fall within the scope of the claims of this application.

Claims

1. An oral care composition with enhanced anti-allergic effect, characterized in that: The components include the following parts by mass: Compound plant extract 0.9-3 parts; 0.5-2 parts of anti-allergy agent; 0.1-0.3 parts of anti-caries agent; Stabilizer 0.01-0.03 parts; pH adjuster 0.08-0.12 parts; The compound plant extract is a combination of licorice extract, gastrodia extract, and agarwood extract; The stabilizer is rosmarinic acid.

2. The oral care composition with enhanced anti-allergy effect according to claim 1, characterized in that: In the compound plant extract, the mass ratio of licorice extract, gastrodia extract, and agarwood extract is 0.3-1:0.3-1:0.3-1.

3. The oral care composition for enhancing anti-sensitivity according to claim 2, characterized in that: The anti-allergy agent is strontium chloride, the anti-caries agent is sodium fluoride, and the pH adjuster is a mixture of citric acid and sodium citrate.

4. The oral care composition for enhancing anti-sensitivity according to claim 2, characterized in that: In the pH adjuster, the mass ratio of citric acid to sodium citrate is 0.04-0.06:0.04-0.

06.

5. The application of an oral care composition with enhanced anti-sensitivity effect according to any one of claims 1-4, characterized in that: The enhanced desensitizing oral care composition is used to prepare toothpaste, the toothpaste comprising the following components in parts by weight: Moisturizer 62.3-63.7 parts; 0.8-1.2 parts adhesive; 19.5-20.5 parts of friction agent; 1.9-2.1 parts of foaming agent; Taste improver 0.15-0.25 parts; Preservative: 0.15-0.25 parts; Fragrance agent 0.9-1.1 parts; Enhanced anti-allergy oral care composition: 1.59-5.45 parts; Solvent: 5.45-12.71 parts.

6. The application of the oral care composition with enhanced anti-allergic effect according to claim 5, characterized in that: The moisturizer is a compound of sorbitol, glycerin and propylene glycol, and the mass ratio of sorbitol, glycerin and propylene glycol is 56.5-57.5: 2.9-3.1: 2.9-3.

1.

7. The application of the oral care composition with enhanced anti-allergic effect according to claim 5, characterized in that: The adhesive is a compound of cellulose gum, xanthan gum, and carrageenan extract, wherein the mass ratio of cellulose gum, xanthan gum, and carrageenan extract is 0.5-0.7:0.15-0.25:0.15-0.

25.

8. The application of the oral care composition with enhanced anti-allergic effect according to claim 5, characterized in that: The abrasive is hydrated silica, the foaming agent is sodium lauryl sulfate, the taste improver is sodium saccharin, the preservative is sodium benzoate, the fragrance is flavoring, and the solvent is water.

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