Stirring reaction kettle for extracting serum protein

Abstract

The invention relates to the technical field of serum albumin extraction, and particularly discloses a stirring reaction kettle for serum protein extraction, the stirring reaction kettle comprises a container tank body and a stirring mechanism, the stirring mechanism comprises a stirring shaft and a wall scraping assembly, and the stirring shaft is rotatably connected with the container tank body; the wall scraping assembly comprises a side wall scraping plate and a bottom wall scraping plate, the side wall scraping plate is used for making contact with the inner side wall of the container tank body, the bottom wall scraping plate is used for making contact with the inner bottom wall of the container tank body, the container tank body comprises a main tank shell and a lifting bottom plate, the lifting bottom plate is slidably connected with the main tank shell, and the sliding direction is parallel to the axial direction of the main tank shell; the plate face of the side, facing the stirring shaft, of the lifting bottom plate is the inner bottom wall face of the container tank body, a discharging through hole is formed in the lifting bottom plate, and a driving assembly used for controlling the lifting bottom plate to slide is arranged on the main body tank shell. The side wall scraping plate and the bottom wall scraping plate are in contact friction with the inner side wall and the inner bottom wall of the container tank body correspondingly, and the overall cleaning effect on the inner wall of the container tank body is improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of serum albumin extraction, and particularly relates to a stirring reaction kettle for serum protein extraction. BACKGROUND

[0002] Serum protein is a very important biochemical standard in blood. By detecting serum protein in blood, the physiological health data of a person can be reflected. Meanwhile, serum protein can be applied to the medical field after extraction. The core process of preparation includes steps such as centrifugal removal of plasma, acidification treatment and organic solvent extraction, and the raw material is derived from animal serum. The extraction of serum protein is a common operation in biochemical, medical research and clinical diagnosis, and is mainly used for proteomics analysis, detection of specific protein expression, quantitative analysis of cytokines, antibodies and other experiments.

[0003] In the prior art, a reaction kettle is usually used to extract serum protein. However, in actual use, due to the characteristics of the extraction raw material, the raw material adheres to the inner wall of the extraction tank during the extraction process and is difficult to remove.

[0004] Therefore, in the related technology, a scraping wall structure is usually arranged at the edge of the stirring shaft of the stirring reaction kettle. For example, a Chinese patent document with publication number CN222961347U discloses a serum albumin extractor, which includes an extraction tank, a connecting pipe arranged at the top of the extraction tank, and a stirring mechanism arranged inside the extraction tank. Two scraping wall parts are arranged in the stirring mechanism to scrape off the extraction raw material adhered to the inner wall of the extraction tank during stirring. The extraction tank can stir the extraction raw material through the design of the stirring mechanism. In addition, the scraping wall parts are arranged in the stirring mechanism. Through the design of the mounting rod, the rotating shaft and the scraper, the extraction raw material adhered to the tank wall can be scraped off. In addition, the scraping wall parts are also provided with a sliding rod, a spring, a mounting frame and a rubber block structure. The extraction raw material adhered to the scraper is shaken off through the inertia generated by the rotation and pause of the stirring mechanism. Thus, the effect of completely removing the adhered raw material from the tank wall is achieved.

[0005] However, the extraction tank is a closed cylindrical tank body. The inner wall includes an inner side wall and an inner bottom wall. According to the position and operating principle of the scraping wall part in the above technical solution, the scraping wall part can only contact and scrape off the adhered material on the inner side wall of the extraction tank, but cannot contact the material adhered or resting on the inner bottom wall of the extraction tank. In addition, the space operating conditions of the inner bottom wall of the extraction tank are poor, so that the cleaning degree of the inner bottom wall of the extraction tank is poor and the cleaning difficulty is high. SUMMARY

[0006] The present application provides a stirring reaction kettle for serum protein extraction, which aims to solve the problem of poor cleaning degree of the inner bottom wall of the extraction tank in the related technology.

[0007] The serum protein extraction stirring reaction kettle of the present application comprises a container tank body and a stirring mechanism, the stirring mechanism comprises a stirring shaft, the stirring shaft is coaxially connected with the container tank body in rotation, a stirring paddle module is arranged on the stirring shaft, the stirring mechanism further comprises a wall scraping assembly, the wall scraping assembly is used for contacting and rubbing the inner wall of the container tank body; the wall scraping assembly comprises a side wall scraper and a bottom wall scraper, the side wall scraper and the bottom wall scraper are connected with the stirring shaft, the side wall scraper is used for contacting the inner side wall of the container tank body, the bottom wall scraper is used for contacting the inner bottom wall of the container tank body, the container tank body comprises a main tank shell and a lifting bottom plate, the lifting bottom plate is located at the bottom of the main tank shell and is slidably connected with the main tank shell, the sliding direction is consistent with the axial direction of the main tank shell, the side plate surface of the lifting bottom plate towards the stirring shaft is the inner bottom wall surface of the container tank body, a discharge through hole is arranged on the lifting bottom plate, and a driving assembly for controlling the sliding of the lifting bottom plate is arranged on the main tank shell.

[0008] The effect is that the side wall scraper and the bottom wall scraper on the stirring shaft are respectively used for contacting and rubbing the inner side wall and the inner bottom wall of the container tank body to clean the adhering objects; during the serum protein separation stirring, the lifting bottom plate is not in contact with the bottom wall scraper, the operation resistance of the stirring mechanism is small, and the damage to the substances in the serum is also small; after the centrifugal stirring is completed and the mixture in the container tank body is discharged, the driving assembly controls the lifting bottom plate to move close to the stirring shaft, the upper plate surface of the lifting bottom plate, that is, the inner bottom wall of the container tank body, is in contact with the bottom wall scraper, the stirring shaft rotates to make the bottom wall scraper rub the whole surface of the lifting bottom plate and the side wall scraper rub the inner side wall surface of the container tank body, so that the inner wall of the container tank body is cleaned.

[0009] Preferably, the main tank shell is fixedly connected with a mounting bottom plate at the bottom, an elastic sealing ring body is coaxially arranged between the mounting bottom plate and the main tank shell, the elastic sealing ring body is in abutment with the mounting bottom plate and the main tank shell at the same time, a buffer cavity is formed between the mounting bottom plate and the lifting bottom plate, the driving assembly comprises a plurality of power cylinders, the power cylinders are mounted on the mounting bottom plate, and the power cylinders apply a pushing and pulling force to the lifting bottom plate.

[0010] Preferably, the main tank shell comprises a tank top cover, an intermediate cylinder and a tank bottom connecting ring body which are coaxially connected in sequence, a plurality of support column bars are arranged between the tank top cover and the tank bottom connecting ring body, the plurality of support column bars are arranged in an array around the circumference of the main tank shell, the support column bars are located outside the intermediate cylinder, and the intermediate cylinder is made of transparent material.

[0011] Its effect lies in: the operator located outside can observe the stirring condition inside the container tank through the intermediate cylinder, on the one hand, the top cover of the tank and the connecting ring body of the tank bottom maintain a relatively stable positional relationship through the support column, reducing the stress of the intermediate cylinder, on the other hand, the support column forms a fence outside the intermediate cylinder, further improving the structural protection of the intermediate cylinder.

[0012] Preferably, a plurality of auxiliary pile pipes are arranged on the lifting bottom plate, the auxiliary pile pipes are fixedly connected with the mounting bottom plate, the length direction of the auxiliary pile pipes is parallel to the moving direction of the lifting bottom plate, the upper end of the auxiliary pile pipe is located above the lifting bottom plate and is lower than the bottom wall scraper, an auxiliary through hole is formed in the lifting bottom plate for the auxiliary pile pipe to pass through, the auxiliary pile pipe is connected with the air source, and air jet capillary holes are formed in the auxiliary pile pipe.

[0013] Its effect lies in: during the centrifugal stirring process and the process of opening the discharge pipe to discharge, the air source continuously inputs air flow into the main tank shell through the air jet capillary holes, so that the material close to the inner bottom wall of the main tank shell has high fluidity and is not easy to form adherents on the inner bottom wall.

[0014] Preferably, a sealing sleeve is coaxially sleeved and fixedly connected outside the auxiliary pile pipe, the outer wall of the sealing sleeve abuts against the hole wall of the auxiliary through hole, the upper end of the sealing sleeve is lower than the air jet capillary hole, a stable clamping ring is integrally formed on the inner side wall of the sealing sleeve, and a embedding ring groove is formed in the outer wall of the auxiliary pile pipe for the stable clamping ring to be embedded.

[0015] Its effect lies in: the sealing sleeve is used to improve the sealing property between the auxiliary pile pipe and the hole wall of the auxiliary through hole, and the cooperation of the embedding ring groove and the stable clamping ring improves the axial stability of the sealing sleeve relative to the auxiliary pile pipe.

[0016] Preferably, a cleaning channel is formed in the auxiliary pile pipe, one port of the cleaning channel is located at the top end of the auxiliary pile pipe, the cleaning channel is used for flowing cleaning liquid, and a closing valve is arranged at the top end of the auxiliary pile pipe and in the cleaning channel.

[0017] Its effect lies in: in the case of insufficient pressure, the cleaning channel is closed by the closing valve, the cleaning channel and the inner cavity of the container tank are isolated from each other, and the centrifugal stirring work in the container tank is not affected; when the container tank is cleaned later, the hydraulic pressure in the cleaning channel can open the closing valve to input the cleaning liquid into the container tank.

[0018] Preferably, the stirring paddle module comprises a stirring crossbar and a spiral blade, one end of the stirring crossbar is connected with the stirring shaft, the other end is connected with the spiral blade, the length direction of the stirring crossbar is the radial direction of the stirring shaft, and the side wall scraper is located at the end of the stirring crossbar away from the stirring shaft.

[0019] Preferably, the side wall scraper and the stirring crossbar are slidingly connected, the sliding direction is parallel to the length direction of the stirring crossbar, the stirring shaft is provided with a transmission assembly, the transmission assembly is used for controlling the movement of the side wall scraper, the transmission assembly comprises a connecting spring connected between the side wall scraper and the stirring crossbar, and a gap is formed between the side wall scraper and the inner side wall of the main tank shell in a natural state.

[0020] The effect is that, in the natural state, the tension of the connecting spring keeps the distance between the side wall scraper and the inner side wall of the container tank, and the friction force of the stirring shaft during rotation is small.

[0021] Preferably, the discharge through hole is eccentrically arranged on the lifting bottom plate, the transmission assembly further comprises a first push rod, a second push rod and a transmission connecting rod, the first push rod is coaxially arranged in the stirring shaft, the second push rod is coaxially arranged in the stirring crossbar and fixedly connected with the side wall scraper, the two ends of the transmission connecting rod are hingedly connected with the first push rod and the second push rod respectively, and the middle part of the lifting bottom plate is coaxially connected with a supporting push rod, the supporting push rod is coaxially arranged in the stirring shaft and used for abutting against the first push rod.

[0022] The effect is that, when the inner side wall of the container tank is cleaned, the supporting push rod moves upward, under the action of the transmission connecting rod, the movement of the first push rod can transmit the pushing force to the second push rod to make the second push rod move, so as to control the transverse movement of the side wall scraper, that is, to control the side wall scraper to contact the inner wall of the middle cylinder.

[0023] Preferably, the main tank shell and the bearing chassis slide relative to each other, the sliding direction is vertical, the bearing chassis is provided with a weighing sensor, and the main tank shell is installed on the detection point of the weighing sensor.

[0024] The effect is that the weighing sensor is used for weighing and measuring the change of the gravity borne by itself, so that the operator can intuitively know the amount of the material in the container tank.

[0025] By adopting the above technical scheme, the present application has the following beneficial effects: By arranging the lifting bottom plate, the bottom wall scraper and the side wall scraper, the inner bottom wall and the inner side wall of the container tank can be contacted and rubbed after being separated and stirred, so that the attached matters can be removed and cleaned, and under the action of the auxiliary pile pipe, the bottom is not easy to produce attachment when the gas flow is sprayed during stirring, and the cleaning liquid can be transported into the main tank shell during cleaning, so that the container tank has high cleaning effect. Attached Figure Description

[0026] Figure 1 This is a schematic diagram illustrating the overall structure of the stirred reaction vessel for serum protein extraction in an embodiment of the present invention.

[0027] Figure 2 This is a cross-sectional schematic diagram illustrating the internal structure of the container in an embodiment of the present invention.

[0028] Figure 3 This is a schematic diagram illustrating the overall structure of the stirring mechanism in an embodiment of the present invention.

[0029] Figure 4 This is a cross-sectional view of the auxiliary pile pipe in an embodiment of the present invention, showing the situation where the lifting base plate is not in contact with the bottom wall scraper.

[0030] Figure 5 This is a cross-sectional view of the auxiliary pile pipe in an embodiment of the present invention, showing the lifting base plate rising and contacting the bottom wall scraper.

[0031] Figure 6 This is a cross-sectional view of the transmission assembly in an embodiment of the present invention, showing the structure of the transmission assembly when the side wall scraper is not in contact with the inner wall of the intermediate cylinder.

[0032] Figure 7 This is a cross-sectional view of the structure in an embodiment of the present invention, showing the contact between the side wall scraper and the inner wall of the intermediate cylinder by the transfer assembly.

[0033] Figure label: 1. Support frame; 11. Weighing sensor; 2. Container body; 21. Main tank shell; 211. Tank top cover; 212. Intermediate cylinder; 213. Tank bottom connecting ring; 22. Support column; 23. Mounting base plate; 231. Discharge through hole; 24. Elastic sealing ring; 241. Buffer cavity; 25. Lifting base plate; 251. Auxiliary through hole; 252. Discharge through hole; 26. Telescopic connecting pipe; 27. Discharge pipe; 3. Auxiliary pile pipe; 31. Embedded ring groove; 32. Air jet capillary hole; 33. Cleaning channel; 331. Sealing valve; 34. Sealing sleeve; 341. Stabilizing retaining ring; 4. Stirring mechanism; 40. Stirring shaft; 401. Stirring motor; 41. Stirring paddle module; 411. Stirring crossbar; 412. Spiral blade; 42. Wall scraping assembly; 421. Side wall scraper; 422. Bottom wall scraper; 43. Transmission assembly; 431. First push rod; 432. Second push rod; 433. Transmission connecting rod; 434. Top support push rod; 435. Connecting spring; 436. Top support cylinder; 5. Drive assembly; 51. Power cylinder. Detailed Implementation

[0034] The following is combined Figures 1 to 7The application discloses a stirring reaction kettle for serum protein extraction.

[0035] The application discloses a stirring reaction kettle for serum protein extraction, which comprises a bearing chassis 1, a container tank 2 and a stirring mechanism 4. Figure 1 and Figure 2 The bearing chassis 1 is placed on the ground, the container tank 2 is installed on the bearing chassis 1, and the stirring mechanism 4 is located on the container tank 2. The container tank 2 is used for containing serum raw materials to be treated, and the stirring mechanism 4 is used for stirring and separating the serum raw materials in the container tank 2. The stirring mechanism 4 comprises a stirring shaft 40 and a wall scraping assembly 42, and the wall scraping assembly 42 is used for contacting and rubbing the inner wall of the container tank 2 after stirring and separation.

[0036] As shown in Figure 1 and Figure 2 , the container tank 2 is in a cylindrical shape, and the axis thereof is in a vertical direction. The container tank 2 comprises a main tank shell 21 and a lifting bottom plate 25. The main tank shell 21 comprises, from top to bottom, a tank top cover 211, an intermediate cylinder 212 and a tank bottom connecting ring 213 which are coaxially connected in sequence. The intermediate cylinder 212 is made of transparent material, and in the embodiment, quartz glass is used. An operator outside can observe the stirring condition in the container tank 2 or the separation condition of the serum raw materials through the intermediate cylinder 212. A plurality of support column rods 22 are arranged between the tank top cover 211 and the tank bottom connecting ring 213. The plurality of support column rods 22 are arranged in an array around the circumference of the main tank shell 21, and the support column rods 22 are located outside the intermediate cylinder 212. On the one hand, the tank top cover 211 and the tank bottom connecting ring 213 maintain a relatively stable positional relationship through the support column rods 22, thereby reducing the stress of the intermediate cylinder 212. On the other hand, the support column rods 22 form a fence outside the intermediate cylinder 212, thereby further improving the structural protection of the intermediate cylinder 212. In the embodiment, the support column rods 22 are made of stainless steel pipes, and the number of the support column rods 22 is eighteen.

[0037] As shown in Figure 1 , the main tank shell 21 and the bearing chassis 1 are relatively arranged in a sliding mode, and the sliding direction is in a vertical direction. Two load sensors 11 are fixedly arranged on the bearing chassis 1. The detection points of the two load sensors 11 are connected with the tank bottom connecting ring 213 at the same time, and the two detection points are located at the transverse edges of the tank bottom connecting ring 213, respectively. When the mixed materials carried in the main tank shell 21 gradually increase, the mixed materials will have an increasingly greater downward trend, and the tank bottom connecting ring 213 will act on the load sensors 11. The load sensors 11 are used for weighing and measuring the change of the gravity borne by the load sensors 11, so that an operator can intuitively know the amount of the materials in the container tank 2.

[0038] As shown in Figure 1 and Figure 2As shown, the lifting bottom plate 25 is located at the bottom of the main tank shell 21 and is in sliding connection with the main tank shell 21, the inner wall of the middle cylinder body 212 is the inner side wall of the main tank shell 21, and the upper plate surface of the lifting bottom plate 25 is the inner bottom wall of the main tank shell 21. The lower side of the tank bottom connecting ring body 213 is coaxially fixedly connected with the mounting bottom plate 23 through a plurality of bolts, the elastic sealing ring body 24 made of rubber is coaxially arranged between the mounting bottom plate 23 and the main tank shell 21, the bolts pass through the mounting bottom plate 23 and the elastic sealing ring body 24 in sequence and are then screwed into the tank bottom connecting ring body 213, and the elastic sealing ring body 24 is continuously subjected to the extrusion action from the mounting bottom plate 23 and the tank bottom connecting ring body 213. The edge of the lifting bottom plate 25 is in contact with the inner edge of the tank bottom connecting ring body 213, a buffer cavity 241 is formed between the mounting bottom plate 23 and the lifting bottom plate 25, the elastic sealing ring body 24 is used to improve the sealing performance of the buffer cavity 241, the driving assembly 5 includes a plurality of power cylinders 51, in this embodiment, the power cylinders 51 are air cylinders, and there are four air cylinders. The four air cylinders are uniformly arranged around the circumference of the mounting bottom plate 23, the cylinder bodies of the four air cylinders are fixedly installed below the mounting bottom plate 23, the piston rods of the air cylinders pass through the mounting bottom plate 23 upwards and are fixedly connected with the lower plate surface of the lifting bottom plate 25, the four air cylinders are synchronously started and stopped, a pushing force or a pulling force is applied to the lifting bottom plate 25, and the lifting and lowering of the lifting bottom plate 25 are controlled.

[0039] As Figure 1 , Figure 2 and Figure 3As shown, the stirring shaft 40 is coaxially connected to the tank top cover 211, and the tank top cover 211 is further fixedly installed with a stirring motor 401 for driving the stirring shaft 40 to rotate. The portion of the stirring shaft 40 that is deep into the container tank 2 is fixedly connected with a stirring paddle module 41, which includes a stirring horizontal rod 411 and a spiral blade 412. One end of the stirring horizontal rod 411 is welded and fixed to the stirring shaft 40, and the other end is welded and fixed to the spiral blade 412. The length direction of the stirring horizontal rod 411 is the radial direction of the stirring shaft 40. The scraping wall assembly 42 includes a bottom wall scraper 422, the length direction of which is parallel to the length direction of the stirring horizontal rod 411. The bottom wall scraper 422 is located at the lower end of the stirring shaft 40 and is also welded and fixed to the stirring shaft 40. There are two bottom wall scrapers 422, which are located at opposite sides of the stirring shaft 40. In this embodiment, there are four stirring horizontal rods 411, which are evenly divided into two groups. The two stirring horizontal rods 411 in a single group are coaxial, and the two groups of stirring horizontal rods 411 are located at different heights. There are two spiral blades 412, which are both in the shape of a spiral around the stirring shaft 40. The upper end of each spiral blade 412 is fixedly connected to the end of one of the stirring horizontal rods 411 at a high position, the middle part is fixedly connected to the end of one of the stirring horizontal rods 411 at a low position, and the bottom end is fixedly connected to the edge of the end of the bottom wall scraper 422 that is away from the stirring shaft 40. The surface of the bottom wall scraper 422 is relatively inclined. During the rotation of the stirring shaft 40, the pushing force of the bottom wall scraper 422 and the spiral blade 412 on the mixture in the container tank 2 both has an upward component. During centrifugal stirring, the lifting bottom plate 25 is at a height position that does not contact the bottom wall scraper 422, i.e., there is a gap between the lifting bottom plate 25 and the bottom wall scraper 422. In this embodiment, the gap between the lifting bottom plate 25 and the bottom wall scraper 422 during centrifugal stirring is 2.5-3 cm.

[0040] As shown in Figure 1 and Figure 2 , the lifting bottom plate 25 is provided with a discharge through hole 252 that is eccentric to the surface of the lifting bottom plate 25. The mounting bottom plate 23 is provided with a discharge through hole 231 that is coaxially aligned with the discharge through hole 252. The mounting bottom plate 23 and the lifting bottom plate 25 are fixedly connected with a telescopic connecting pipe 26, which is composed of two short straight pipes that are coaxially sleeved. The two ends of the telescopic connecting pipe 26 are respectively connected to the discharge through hole 252 and the discharge through hole 231. The lower part of the mounting bottom plate 23 is fixedly connected with an L-shaped discharge pipe 27, which is provided with a stop valve. After the centrifugal stirring of the mixture in the container tank 2 is completed, the mixture is discharged in sequence through the discharge through hole 252, the telescopic connecting pipe 26, the discharge through hole 231, and the discharge pipe 27.

[0041] As shown in Figure 1 , Figure 2 , Figure 4 and Figure 5As shown, a plurality of auxiliary stake pipes 3 are arranged on the lifting base plate 25, and the auxiliary stake pipes 3 are fixedly connected with the mounting base plate 23. The length direction of the auxiliary stake pipes 3 is parallel to the moving direction of the lifting base plate 25. During the centrifugal stirring process, the lifting base plate 25 is in a low position, and at this time, the upper end of the auxiliary stake pipe 3 is located above the lifting base plate 25 and is lower than the bottom wall scraper 422. The upper end of the auxiliary stake pipe 3 is about 1 cm higher than the plate surface of the lifting base plate 25. A plurality of auxiliary through holes 251 for the auxiliary stake pipes 3 to pass through are arranged on the lifting base plate 25. The outer coaxial sleeve of the auxiliary stake pipe 3 is fixedly bonded with a sealing sleeve 34 made of rubber material. The outer wall of the sealing sleeve 34 abuts against the hole wall of the auxiliary through hole 251 to improve the sealing performance between the auxiliary stake pipe 3 and the hole wall of the auxiliary through hole 251. During the movement of the lifting base plate 25, the sealing sleeve 34 and the hole wall of the auxiliary through hole 251 have a friction sliding trend. Therefore, a stable clamping ring 341 is integrally formed on the inner side wall of the sealing sleeve 34. An embedding ring groove 31 is arranged on the outer wall of the auxiliary stake pipe 3, and the stable clamping ring 341 is embedded in the embedding ring groove 31, thereby improving the axial stability between the sealing sleeve 34 and the auxiliary stake pipe 3.

[0042] As shown in Figure 4 and Figure 5 , the auxiliary stake pipe 3 is provided with a jet capillary hole 32. The jet capillary hole 32 is located on the side wall of the auxiliary stake pipe 3, and the upper end of the sealing sleeve 34 is lower than the hole of the jet capillary hole 32. In this embodiment, the number of jet capillary holes 32 on a single auxiliary stake pipe 3 is four. The length direction of the jet capillary hole 32 is consistent with the length direction of the auxiliary stake pipe 3 and is eccentric to the auxiliary stake pipe. The four jet capillary holes 32 are arranged in an array around the circumference of the auxiliary stake pipe 3. The auxiliary stake pipe 3 is connected with a nitrogen gas source (not shown in the figure) through the jet capillary hole 32. During the centrifugal stirring process and the process of opening the discharge pipe 27 to discharge, the hole position of the jet capillary hole 32 is located above the upper plate surface of the lifting base plate 25. The nitrogen gas source with a certain pressure continuously inputs gas flow into the main tank shell 21 through the jet capillary hole 32. The nitrogen gas flow directly contacts the serum mixture at the bottom of the main tank shell 21, so that the material close to the inner bottom wall of the main tank shell 21 has high fluidity, thereby preventing the formation of adhering materials on the inner bottom wall.

[0043] As shown in Figure 4 and Figure 5As shown, the auxiliary pile pipe 3 is provided with a cleaning channel 33, the extension direction of the cleaning channel 33 is consistent with the length direction of the auxiliary pile pipe 3 and the two are coaxial, one end of the cleaning channel 33 is located at the top end of the auxiliary pile pipe 3, the cleaning channel 33 is used for flowing cleaning liquid, and a device (not shown in the figure) for pumping cleaning liquid into the cleaning channel 33 is arranged outside the equipment. A rubber sealing flap 331 is arranged at the top end of the auxiliary pile pipe 3 and in the cleaning channel 33, and the sealing flap 331 has a one-way conduction performance. During centrifugal stirring, the sealing flap 331 seals the upper port of the cleaning channel 33, and the cleaning channel 33 and the inner cavity of the container tank body 2 are isolated from each other. After centrifugal stirring, the cleaning process of the container tank body 2 is carried out, the lifting bottom plate 25 rises to the contact of the plate surface and the bottom wall scraper 422, at this time the upper hole of the auxiliary through hole 251 is higher than the upper end of the auxiliary pile pipe 3, the cleaning liquid is conveyed into the container tank body 2 through the cleaning channel 33, the pressure of the cleaning liquid pushes open the sealing flap 331 into the auxiliary through hole 251 and finally reaches the space above the lifting bottom plate 25, thereby improving the cleaning effect of the inside of the container tank body 2. When the cleaning liquid is stopped, the pushing force that pushes open the sealing flap 331 disappears, the elastic restoring force of the rubber makes the sealing flap 331 quickly rebound to the initial position and reseal. When the cleaning liquid is discharged, the lifting bottom plate 25 is controlled to descend, so that the upper end of the auxiliary pile pipe 3 is again located above the lifting bottom plate 25. In this way, the complete discharge of the cleaning liquid is ensured.

[0044] As shown in Figure 2 , Figure 6 and Figure 7 , the side wall scraper 421 is located at the end of the stirring cross rod 411 away from the stirring shaft 40, and the side wall scraper 421 has two and is located at the opposite sides of the stirring shaft 40 respectively. The side wall scraper 421 and the stirring cross rod 411 are slidingly connected, the sliding direction is parallel to the length direction of the stirring cross rod 411, and during centrifugal stirring, the side wall scraper 421 does not contact the inner wall of the middle cylinder 212; the stirring shaft 40 is provided with a transmission assembly 43 for controlling the movement of the side wall scraper 421. The transmission assembly 43 includes a connecting spring 435 connected between the side wall scraper 421 and the stirring cross rod 411 at the low position, and in the natural state, a gap of about 3 cm is formed between the side wall scraper 421 and the inner side wall of the main tank shell 21.

[0045] As shown in Figure 6 and Figure 7As shown, the transmission assembly 43 further comprises a first push rod 431, a second push rod 432 and a transmission connecting rod 433, the first push rod 431 is coaxially arranged in the stirring shaft 40, the second push rod 432 is coaxially arranged in the stirring cross rod 411 at a lower position and is fixedly connected with the side wall scraper 421 at one end, so that the sliding directions of the first push rod 431 and the second push rod 432 are perpendicular to each other; the two ends of the transmission connecting rod 433 are hingedly connected with the first push rod 431 and the second push rod 432 respectively, and the hinge axes are in horizontal directions. Under the action of the transmission connecting rod 433, the movement of the first push rod 431 can transmit a pushing force to the second push rod 432 to move the second push rod 432, so as to control the transverse movement of the side wall scraper 421, that is, whether the side wall scraper 421 contacts the inner wall of the middle cylinder 212. The middle part of the lifting bottom plate 25 is coaxially and slidingly connected with a jacking push rod 434, the jacking push rod 434 is coaxially arranged in the stirring shaft 40 and is used to abut against the bottom end of the first push rod 431. The jacking cylinder 436 is fixedly connected on the mounting bottom plate 23, the piston rod of the jacking cylinder 436 is parallel to the moving direction of the jacking push rod 434, and the piston rod of the jacking cylinder 436 is fixedly connected with the bottom end of the jacking push rod 434. The lifting movement of the jacking push rod 434 and the lifting movement of the lifting bottom plate 25 are independent of each other, so as to facilitate independent control of the use state of the side wall scraper 421 and the bottom wall scraper 422, and the separate use of the side wall scraper 421 or the bottom wall scraper 422 is beneficial to reducing the rotating resistance of the stirring shaft 40 and improving the stability of the scraping wall assembly 42 during operation.

[0046] Working process of the embodiment: The serum raw material is added into the container jar 2, and the stirring motor 401 is started to perform centrifugal stirring, in this process, the lifting bottom plate 25 is at a low position, the side wall scraper 421 does not contact the inner wall of the middle cylinder 212, the bottom wall scraper 422 does not contact the lifting bottom plate 25, and at the same time, the jet capillary hole 32 of the auxiliary stake pipe 3 continuously sprays nitrogen to improve the fluidity of the mixture near the bottom. After centrifugal stirring is completed, the serum is discharged through the discharge through hole 252, and then the lifting bottom plate 25 or the jacking push rod 434 is controlled to move upward, so that the bottom wall scraper 422 contacts the lifting bottom plate 25 or the side wall scraper 421 contacts the inner wall of the middle cylinder 212, and at the same time, the cleaning liquid is input into the container jar 2 through the cleaning channel 33 of the auxiliary stake pipe 3, and then the stirring shaft 40 is started again to clean the inner wall of the container by friction.

[0047] Although the embodiments of the present application have been shown and described above, it is to be understood that the above description of the embodiments and the accompanying drawings are exemplary and explanatory in nature and are intended to be within the scope of the present application, and the ordinary skill in the art can make changes, modifications, replacements and variations to the above embodiments within the scope of the present application; based on the embodiments of the present application, all other embodiments obtained by the ordinary skill in the art without creative labor are within the scope of protection of the present application.

Claims

1. A serum protein extraction stirring reaction kettle, comprising a container tank body (2) and a stirring mechanism (4), the stirring mechanism (4) comprising a stirring shaft (40), the stirring shaft (40) and the container tank body (2) are coaxially connected, the stirring shaft (40) is provided with a stirring paddle module (41), the stirring mechanism (4) further comprises a wall scraping assembly (42), the wall scraping assembly (42) is used for contacting and rubbing the inner wall of the container tank body (2); characterized in that The wall scraping assembly (42) comprises a side wall scraper (421) and a bottom wall scraper (422), the side wall scraper (421) and the bottom wall scraper (422) are connected with the stirring shaft (40), the side wall scraper (421) is used for contacting the inner side wall of the container tank body (2), the bottom wall scraper (422) is used for contacting the inner bottom wall of the container tank body (2), the container tank body (2) comprises a main tank shell (21) and a lifting bottom plate (25), the lifting bottom plate (25) is located at the bottom of the main tank shell (21) and is slidingly connected with the main tank shell (21), the sliding direction is consistent with the axial direction of the main tank shell (21), the side plate surface of the lifting bottom plate (25) facing the stirring shaft (40) is the inner bottom wall surface of the container tank body (2), a discharge through hole (252) is formed in the lifting bottom plate (25), and a driving assembly (5) for controlling the sliding of the lifting bottom plate (25) is arranged on the main tank shell (21).

2. The stirring reaction vessel for extracting serum protein according to claim 1, wherein The main tank shell (21) is fixedly connected with a mounting bottom plate (23), an elastic sealing ring body (24) is coaxially arranged between the mounting bottom plate (23) and the main tank shell (21), the elastic sealing ring body (24) abuts against the mounting bottom plate (23) and the main tank shell (21) at the same time, a buffer cavity (241) is formed between the mounting bottom plate (23) and the lifting bottom plate (25), the driving assembly (5) comprises a plurality of power cylinders (51), the power cylinders (51) are mounted on the mounting bottom plate (23), and the power cylinders (51) apply a pushing and pulling force to the lifting bottom plate (25).

3. The agitator reactor for serum protein extraction according to claim 2, wherein The main tank shell (21) comprises a tank top cover (211), an intermediate cylinder (212) and a tank bottom connecting ring body (213) which are coaxially connected in sequence, a plurality of support column rods (22) are arranged between the tank top cover (211) and the tank bottom connecting ring body (213), the plurality of support column rods (22) are arranged in an array around the circumference of the main tank shell (21), the support column rods (22) are located outside the intermediate cylinder (212), and the intermediate cylinder (212) is made of transparent material.

4. The stirring reaction vessel for extracting serum protein according to claim 2 or 3, wherein The lifting bottom plate (25) is provided with a plurality of auxiliary pile pipes (3), the auxiliary pile pipes (3) are fixedly connected with the mounting bottom plate (23), the length direction of the auxiliary pile pipes (3) is parallel to the moving direction of the lifting bottom plate (25), the upper end of the auxiliary pile pipes (3) is located above the lifting bottom plate (25) and is lower than the bottom wall scraper (422), the lifting bottom plate (25) is provided with an auxiliary through hole (251) for the auxiliary pile pipes (3) to pass through, the auxiliary pile pipes (3) are connected with the air source, the auxiliary pile pipes (3) are provided with air injection capillary holes (32), and the air injection capillary holes (32) are located on the side wall of the auxiliary pile pipes (3) and are higher than the lifting bottom plate (25).

5. The agitator reactor for serum protein extraction according to claim 4, wherein The auxiliary pile pipes (3) are coaxially sleeved with and fixedly connected with sealing sleeves (34), the outer wall of the sealing sleeves (34) abuts against the hole wall of the auxiliary through hole (251), the upper end of the sealing sleeves (34) is lower than the air injection capillary holes (32), the inner side wall of the sealing sleeves (34) is integrally formed with a stable clamping ring (341), the outer wall of the auxiliary pile pipes (3) is provided with an embedded ring groove (31), and the stable clamping ring (341) is embedded in the embedded ring groove (31).

6. The stirring reaction vessel for extracting serum protein according to claim 4, wherein The auxiliary pile pipes (3) are provided with cleaning channels (33), one of the ports of the cleaning channels (33) is located at the top end of the auxiliary pile pipes (3), the cleaning channels (33) are used for flowing cleaning liquid, and the top end of the auxiliary pile pipes (3) is provided with a closed valve (331) in the cleaning channel (33).

7. The stirring reaction vessel for extracting serum protein according to claim 1 or 2, wherein The stirring paddle module (41) comprises a stirring horizontal rod (411) and a spiral blade (412), one end of the stirring horizontal rod (411) is connected with the stirring shaft (40), the other end is connected with the spiral blade (412), the length direction of the stirring horizontal rod (411) is the radial direction of the stirring shaft (40), and the side wall scraper (421) is located at the end, away from the stirring shaft (40), of the stirring horizontal rod (411).

8. The stirring reaction vessel for extracting serum protein according to claim 7, wherein The side wall scraper (421) and the stirring horizontal rod (411) are slidingly connected, the sliding direction is parallel to the length direction of the stirring horizontal rod (411), the stirring shaft (40) is provided with a transmission assembly (43), the transmission assembly (43) is used for controlling the movement of the side wall scraper (421), the transmission assembly (43) comprises a connecting spring (435), the connecting spring (435) is connected between the side wall scraper (421) and the stirring horizontal rod (411), and in a natural state, a gap is formed between the side wall scraper (421) and the inner side wall of the main body tank shell (21).

9. The agitator reactor for serum protein extraction according to claim 8, wherein The discharge through hole (252) is eccentrically arranged on the lifting bottom plate (25), the transmission assembly (43) further comprises a first push rod (431), a second push rod (432) and a transmission connecting rod (433), the first push rod (431) is coaxially arranged in the stirring shaft (40), the second push rod (432) is coaxially arranged in the stirring cross rod (411) and is fixedly connected with the side wall scraper (421), the two ends of the transmission connecting rod (433) are respectively hinged with the first push rod (431) and the second push rod (432), and the middle part of the lifting bottom plate (25) is coaxially connected with a supporting push rod (434), the supporting push rod (434) is coaxially arranged in the stirring shaft (40) and is used for abutting against the first push rod (431).

10. The agitator reactor for serum protein extraction according to claim 1 or 2, wherein Further comprising a bearing chassis (1), the main body tank shell (21) and the bearing chassis (1) slide relative to each other, the sliding direction is vertical, the bearing chassis (1) is provided with a weighing sensor (11), and the main body tank shell (21) is installed on the detection point of the weighing sensor (11).

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