Deep sea in-situ culture device

Abstract

The invention relates to the technical field of deep-sea microorganisms, and particularly discloses a deep-sea in-situ culture device which comprises a protective shell, a cavity is formed in the protective shell, a water inlet and a gas conveying mechanism are arranged on the cavity, the gas conveying mechanism comprises a gas outlet pipe, a pressure swing adsorption oxygen generator is connected to the protective shell, and the pressure swing adsorption oxygen generator is connected to the protective shell. A first gas conveying pipe is connected to the pressure swing adsorption oxygen generator, a gas concentration detector and an exhaust pipe are connected to the first gas conveying pipe, a gas outlet pipe is arranged on the exhaust pipe, a protection plate and a culture mechanism are further arranged on the protection shell, and the culture mechanism comprises a first culture tank, a second culture tank and a third culture tank. According to the device, the gas conveying mechanism is matched with three independent culture units of a first culture tank, a second culture tank and a third culture tank in the culture mechanism, so that the device has the capability of carrying out a multi-dimensional experiment, and the suitability of the device to deep-sea biology multi-dimensional research requirements is remarkably improved; and an efficient experimental platform is provided for living strategies of organisms in an extreme environment.

Description

Technical Field

[0001] This invention relates to the field of deep-sea microbial technology, and more particularly to a deep-sea in-situ culture device. Background Technology

[0002] The deep-sea ecosystem is rich in biodiversity. Different species (such as chemosynthetic bacteria, photosynthetic algae, and benthic invertebrates) have significantly different requirements for their living environment. The same species also exhibits dynamic changes in its response to parameters such as light, nutrients, and gases at different growth stages.

[0003] Traditional apparatuses often consist of multiple culture units that are structurally and functionally identical "replicas," allowing for repetitive experiments under the same environmental parameters. They cannot be tailored to the specific characteristics of different research subjects. For example, studying the metabolic characteristics of anaerobic microorganisms or photophobic organisms requires simulating low-light environments at specific wavelengths, while researching algae to explore photosynthetic adaptation mechanisms and symbiotic relationships requires different light gradient environments. However, traditional apparatuses cannot simultaneously achieve these specific conditions in different culture units, necessitating batch-by-batch studies of multiple species. This not only prolongs the experimental cycle but may also introduce additional errors due to environmental fluctuations between batches. This limitation makes it difficult to meet the "multi-species, multi-parameter, multi-dimensional" experimental needs of deep-sea biology research, hindering the efficient exploration of the adaptation differences of different biological groups to extreme environments and restricting in-depth analysis of interspecies interactions within deep-sea ecosystems. Summary of the Invention

[0004] The purpose of this invention is to provide a deep-sea in-situ culture device to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, the present invention provides the following technical solution:

[0006] A deep-sea in-situ culture device includes a protective shell with a cavity, and the cavity is equipped with a water inlet and a gas delivery mechanism.

[0007] In a preferred embodiment, the cavity is provided with a limiting block.

[0008] Furthermore, the electric cylinder drives the piston rod, causing the baffle to disengage from the limit block, opening the water inlet, and allowing seawater to flow into the water inlet pipe through the cavity; according to experimental requirements, the corresponding electric valve one is opened to inject seawater into the first culture tank, the second culture tank, or the third culture tank until the preset liquid level is reached, after which the electric valve one is closed, and at the same time the electric cylinder drives the baffle to reset, closing the water inlet to prevent external seawater from continuously rushing in.

[0009] In a preferred embodiment, the cavity is equipped with a sensor.

[0010] Specifically, the sensors are environmental parameter sensors, including temperature sensors, pressure sensors, salinity sensors, dissolved oxygen sensors, and pH sensors, which can monitor changes in environmental parameters around the deep-sea in-situ culture device in real time, providing accurate environmental data support for culture experiments.

[0011] In a preferred embodiment, the protective shell is equipped with an electric cylinder, which is driven by a piston rod to connect to a baffle.

[0012] Specifically, one end of the baffle is in close contact with the limiting block.

[0013] In a preferred embodiment, the gas delivery mechanism includes an outlet pipe, a pressure swing adsorption (PSA) oxygen generator is connected to the protective shell, a gas delivery pipe is connected to the PSA oxygen generator, a gas concentration detector and an exhaust pipe are connected to the gas delivery pipe, and an outlet pipe is provided on the exhaust pipe.

[0014] Furthermore, the pressure swing adsorption oxygen generator is started (pressure swing adsorption oxygen generator is existing technology and will not be described in detail in this invention), and the generated oxygen is transported through the first gas supply pipe, and the oxygen concentration is monitored by the gas concentration detector; if it is necessary to fill the culture tank with oxygen, the corresponding electric air control valve is opened, and the oxygen enters the target culture tank (such as the first culture tank) through the second gas supply pipe, and is monitored in real time by the gas concentration detector.

[0015] Specifically, the air outlet pipe is located inside the cavity.

[0016] In a preferred embodiment, the protective shell is further provided with a protective plate and a culture mechanism, the culture mechanism including a first culture tank, a second culture tank and a third culture tank.

[0017] Furthermore, based on the needs of the culture target (such as microorganisms or algae), the electric valve two of the corresponding storage tank is opened, and the culture medium in the storage tank is injected into the target culture tank (such as the second culture tank) through the infusion tube. The injection volume is ensured to be accurate through flow control.

[0018] Furthermore, the sensors (temperature, pressure, salinity, pH, etc.) are set to collect real-time environmental data of the cavity and the surrounding environment of the culture tank. If the parameters deviate from the preset range (such as abnormal pH value), they can be corrected by injecting the corresponding regulating liquid (such as buffer solution, which is pre-stored in the storage tank) or adjusting the gas flow rate (such as CO2). For photosynthetic algae in the second culture tank, LED lights can be turned on to simulate deep-sea lighting conditions, and the growth status can be recorded by the monitor.

[0019] Furthermore, the biological activities (such as changes in microbial communities, algal growth, and feeding behavior of organisms) inside the culture tank are captured on time or continuously recorded by a monitor (high-definition camera). The video data is transmitted to the surface platform via an underwater communication system (the communication system is a conventional existing technology, which will not be described in detail in this invention). The filter screen of the third culture tank can intercept large particulate impurities to ensure a clean culture environment. Some waste liquid is discharged periodically through the drain valve to maintain water quality stability.

[0020] Specifically, the first culture tank, the second culture tank, and the third culture tank are connected by the protective plate.

[0021] In a preferred embodiment, the first culture tank, the second culture tank, and the third culture tank are all equipped with a drain valve and a monitor.

[0022] In a preferred embodiment, the second culture vessel is also equipped with a lighting lamp.

[0023] In a preferred embodiment, the third culture tank is also equipped with a filter screen.

[0024] Specifically, the lighting includes, but is not limited to, LED lighting.

[0025] Specifically, the monitor includes, but is not limited to, a high-definition camera.

[0026] In a preferred embodiment, the cavity is provided with a water inlet pipe, and the water inlet pipe is provided with at least three sets of electric valves. The water inlet pipe is respectively connected to a first culture tank, a second culture tank, and a third culture tank.

[0027] In a preferred embodiment, the protective shell is provided with a liquid storage chamber, the liquid storage chamber is provided with an inlet and an inlet pipe, the inlet pipe is provided with an electric valve, and the inlet pipe is connected to a first culture tank, a second culture tank or a third culture tank.

[0028] Specifically, at least one set of the liquid storage tank is provided, preferably three sets, corresponding to the first culture tank, the second culture tank and the third culture tank respectively, and connected to each other through infusion pipes.

[0029] Specifically, the infusion port is equipped with a sealing cap to close the channel when not in operation, preventing impurities from entering.

[0030] Specifically, different liquids are pre-stored in the storage tanks, including but not limited to microbial culture medium (liquid), algae culture medium, and biological feed suspension.

[0031] In a preferred embodiment, the other end of the gas supply pipe one is connected to a gas supply pipe two, which is equipped with a gas concentration detector and an electric air control valve. The gas supply pipe two is connected to a first culture tank, a second culture tank, and a third culture tank.

[0032] Furthermore, sensors are installed on the first, second, and third culture tanks, and these sensors, in conjunction with a monitor, record the final environmental parameters.

[0033] Specifically, the electric air control valve can control whether oxygen gas is introduced into the first, second, and third culture tanks.

[0034] Furthermore, if the gas concentration detector displays an abnormality (such as insufficient oxygen purity), it triggers the pressure swing adsorption oxygen generator to perform a self-test or switch to a backup gas source; the anti-corrosion and wear-resistant coatings on the protective plates and exposed parts can reduce seawater corrosion and particulate wear, and after long-term operation, the surface can be cleaned and maintained by a remotely operated vehicle (ROV).

[0035] Specifically, the protective shell, water inlet, baffle, protective plate, and exposed components such as the first culture tank, second culture tank, and third culture tank are all coated with an anti-corrosion and wear-resistant coating; preferably, polytetrafluoroethylene (PTFE) coating, ceramic matrix composite coating, or diamond-like carbon (DLC) coating material is used for coating.

[0036] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0037] This invention, through a gas delivery mechanism and in conjunction with three independent culture units (first, second, and third culture tanks) in the culture mechanism, endows them with differentiated environmental control functions (such as illumination, filtration, and sealed darkness), enabling them to conduct multi-dimensional experiments. This significantly improves the adaptability of the device to the multi-dimensional research needs of deep-sea biology and provides an efficient experimental platform for the survival strategies of organisms in extreme environments.

[0038] This invention utilizes a pressure swing adsorption (PSA) oxygen generator and a gas delivery mechanism to flexibly regulate the concentration of gases such as oxygen and carbon dioxide and precisely input them into the cavity or each culture tank. Combined with the customized culture medium (such as microbial culture medium, algal culture medium, etc.) injected as needed into the storage tank, it achieves multi-parameter coordinated dynamic control of the living environment of the cultured organisms. By reproducing the in-situ physicochemical conditions of the deep sea, it provides a realistic and controllable growth environment for deep-sea microorganisms, algae, and small organisms, effectively avoiding experimental deviations caused by distortion of key parameters such as pressure, temperature, salinity, and gas composition in traditional laboratory simulations, and ensuring the ecological authenticity of the research data. Attached Figure Description

[0039] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort. In the drawings:

[0040] The accompanying drawings described herein are for illustrative purposes only and are not intended to limit the scope of the invention in any way. Furthermore, the shapes and proportions of the components in the drawings are merely illustrative to aid in understanding the invention and do not specifically limit the shapes and proportions of the components. Those skilled in the art, guided by the teachings of this invention, can select various possible shapes and proportions to implement the invention according to specific circumstances.

[0041] Figure 1 This is a schematic diagram of the frontal cross-sectional structure of the deep-sea in-situ culture device proposed in this invention;

[0042] Figure 2 This is a schematic diagram of a partial cross-section of the front elevation of the deep-sea in-situ culture device proposed in this invention;

[0043] Figure 3 This is a schematic diagram of the structure of the first culture tank, the second culture tank, and the third culture tank of the deep-sea in-situ culture device proposed in this invention;

[0044] Figure 4 This is a magnified schematic diagram of node A in the deep-sea in-situ culture device proposed in this invention.

[0045] Figures 1-4 In the accompanying drawings, the reference numerals include:

[0046] 1. Protective shell; 2. Cavity; 3. Water inlet; 4. Limiting block; 5. Electric cylinder; 6. Baffle; 7. Sensor; 8. Pressure swing adsorption oxygen generator; 9. Gas delivery pipe one; 10. Gas concentration detector; 11. Exhaust pipe; 12. Gas outlet pipe; 13. First culture tank; 14. Second culture tank; 15. Third culture tank; 16. Water inlet pipe; 17. Electric valve one; 18. Gas delivery pipe two; 19. Electric air control valve; 20. Liquid storage tank; 21. Infusion port; 22. Infusion pipe; 23. Electric valve two; 24. Drain valve; 25. Monitor; 26. Lighting lamp; 27. Filter screen; 28. Protective plate. Detailed Implementation

[0047] To make the objectives, features, and advantages of this invention more apparent and understandable, the technical solutions of the embodiments of this invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the embodiments described below are only some embodiments of this invention, and not all embodiments. Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this invention.

[0048] In the description of this invention, it should be understood that when a component is considered to be "connected" to another component, it can be directly connected to the other component or there may be an intermediate component present simultaneously. When a component is considered to be "set" on another component, it can be directly set on the other component or there may be an intermediate component present simultaneously.

[0049] Furthermore, terms such as “long,” “short,” “inner,” and “outer” indicate orientation or positional relationships based on the orientation or positional relationships shown in the accompanying drawings. They are used only for the purpose of describing the present invention and are not intended to indicate or imply that the device or component referred to must have this specific orientation or operate in a specific orientational configuration. Therefore, they should not be construed as limitations of the present invention.

[0050] The present invention will now be described in detail with reference to the specific embodiments shown in the accompanying drawings. However, these embodiments do not limit the present invention, and any structural, methodological, or functional modifications made by those skilled in the art based on these embodiments are included within the scope of protection of the present invention.

[0051] See Figure 1-4 The deep-sea in-situ culture device provided in this embodiment includes a protective shell 1, a cavity 2 on the protective shell 1, and a water inlet 3 and a gas delivery mechanism on the cavity 2.

[0052] A limiting block 4 is provided on the cavity 2.

[0053] The cavity 2 is equipped with a sensor 7.

[0054] Specifically, sensor 7 is an environmental parameter sensor, which includes a temperature sensor, a pressure sensor, a salinity sensor, a dissolved oxygen sensor, and a pH sensor. It can monitor changes in environmental parameters around the deep-sea in-situ culture device in real time, providing accurate environmental data support for the culture experiment.

[0055] The protective shell 1 is equipped with an electric cylinder 5, which is connected to a baffle 6 via a piston rod.

[0056] Specifically, one end of the baffle 6 is in close contact with the limiting block 4.

[0057] The gas delivery mechanism includes an outlet pipe 12, a pressure swing adsorption oxygen generator 8 connected to the protective shell 1, a gas delivery pipe 9 connected to the pressure swing adsorption oxygen generator 8, a gas concentration detector 10 and an exhaust pipe 11 connected to the gas delivery pipe 9, and an outlet pipe 12 provided on the exhaust pipe 11.

[0058] In this embodiment, the device is mounted in the target deep-sea area (such as a hydrothermal vent area, cold seep area, or seabed at a specific depth) and secured by anchoring or gravity to ensure the stable placement of the protective shell 1. The status of each component is checked: The drive components, such as the electric cylinder 5, electric valve 17, electric valve 23, and electric air control valve 19, are confirmed to be in their initial closed state; the liquid storage tank 20 is filled with the corresponding liquid (such as microbial culture medium, algae culture medium, etc.) according to the preset experimental requirements, and the infusion port 21 is sealed in advance; the pressure swing adsorption oxygen generator 8, sensor 7, monitor 25 (high-definition camera), and lighting 26 are powered on for self-testing to ensure normal operation.

[0059] Furthermore, the electric cylinder 5 is controlled by an external system (the external system is conventional existing technology, which will not be described in detail in this invention) to drive the piston rod, thereby causing the baffle 6 to disengage from the limiting block 4, opening the water inlet 3, and allowing seawater to flow into the water inlet pipe 16 through the cavity 2 (the water inlet pipe 16 is provided with three sets, each of which is equipped with an electric valve 17, and the three sets of water inlet pipes 16 correspond to the first culture tank 13, the second culture tank 14, or the third culture tank 15, respectively).

[0060] According to the experimental requirements, open the corresponding electric valve 17 to inject seawater into the first culture tank 13, the second culture tank 14, or the third culture tank 15 until the preset liquid level is reached. Then close the electric valve 17. At the same time, the electric cylinder 5 drives the baffle 6 to reset and close the water inlet 3 to prevent external seawater from continuously flowing in.

[0061] Furthermore, the pressure swing adsorption oxygen generator 8 (which is conventional prior art and will not be described in detail here) is started through an external system (the external system is conventional prior art and will not be described in detail here). The pressure swing adsorption oxygen generator is equipped with an oxygen cylinder to generate oxygen. The generated oxygen is delivered through the first gas delivery pipe 9, and the oxygen concentration is monitored by the gas concentration detector 10. If it is necessary to fill the culture tank with oxygen, the corresponding electric air control valve 19 is opened, and the oxygen enters the target culture tank (such as the first culture tank 13) through the second gas delivery pipe 18. The gas concentration is monitored in real time by the gas concentration detector.

[0062] Specifically, the vent pipe 12 is located inside the cavity 2.

[0063] The protective shell 1 is also equipped with a protective plate 28 and a culture mechanism, which includes a first culture tank 13, a second culture tank 14 and a third culture tank 15.

[0064] Specifically, the first culture tank 13, the second culture tank 14, and the third culture tank 15 are connected by a protective plate 28.

[0065] The first culture tank 13, the second culture tank 14 and the third culture tank 15 are all equipped with a drain valve 24 and a monitor 25;

[0066] The second culture tank 14 is also equipped with a lighting lamp 26;

[0067] The third culture tank 15 is also equipped with a filter screen 27.

[0068] Specifically, the lighting 26 includes, but is not limited to, LED lighting.

[0069] Specifically, the monitor 25 includes, but is not limited to, a high-definition camera.

[0070] The cavity 2 is provided with a water inlet pipe 16, and the water inlet pipe 16 is provided with at least three sets of electric valves 17. The water inlet pipe 16 is connected to the first culture tank 13, the second culture tank 14 and the third culture tank 15 respectively.

[0071] The protective shell 1 is provided with a liquid storage tank 20, the liquid storage tank 20 is provided with a liquid inlet 21 and a liquid inlet pipe 22, the liquid inlet pipe 22 is provided with an electric valve 23, and the liquid inlet pipe 22 is connected to a first culture tank 13, a second culture tank 14 or a third culture tank 15.

[0072] Specifically, the liquid storage tank 20 is provided with at least one set, preferably three sets, corresponding to the first culture tank 13, the second culture tank 14 and the third culture tank 15 respectively, and connected to each other through the infusion pipe 22.

[0073] Specifically, a sealing cap is provided at infusion port 21 to close the channel when not in operation and prevent impurities from entering.

[0074] Specifically, the storage tank 20 is pre-stored with different liquids, including but not limited to microbial culture medium, algae culture medium, and biological feed suspension.

[0075] Furthermore, according to the needs of the culture object (such as microorganisms or algae), the electric valve 23 of the corresponding storage tank 20 is opened, and the culture medium in the storage tank is injected into the target culture tank (such as the second culture tank 14) through the infusion pipe 22. The injection volume is ensured to be accurate through flow control.

[0076] Furthermore, the sensor 7 (temperature, pressure, salinity, pH, etc.) is set to collect real-time environmental data of the cavity 2 and the surrounding environment of the culture tank. If the parameters deviate from the preset range (such as abnormal pH value), they can be corrected by injecting the corresponding regulating liquid (such as buffer solution, which is pre-stored in the storage tank) or adjusting the gas flow rate (such as CO2). For photosynthetic algae in the second culture tank 14, the LED lighting 26 can be turned on to simulate deep-sea lighting conditions, and the growth status can be recorded by the monitor 25.

[0077] Although the deep sea lacks sunlight, there are still a few special types of algae. Dinoflagellates and diatoms exist in the mesophyll (microphotosphere) at depths of 200-1000 meters.

[0078] Furthermore, the monitoring device 25 (high-definition camera) takes photos or records videos of biological activities (such as changes in microbial communities, algal growth, and feeding behavior of biological food) in the culture tank at regular intervals. The video data is transmitted to the surface platform via an underwater communication system (the communication system is conventional existing technology and will not be described in detail in this invention). The filter screen 27 of the third culture tank 15 can intercept large particulate impurities to ensure a clean culture environment. Some waste liquid is discharged periodically through the drain valve 24 to maintain water quality stability.

[0079] Furthermore, if the gas concentration detector 10 displays an abnormality (such as insufficient oxygen purity), it triggers the pressure swing adsorption oxygen generator 8 to perform a self-test or switch to a backup gas source; the anti-corrosion and wear-resistant coatings on the protective plate 28 and each exposed component can reduce seawater corrosion and particulate wear, and after long-term operation, the surface can be cleaned and maintained by a remotely operated vehicle (ROV).

[0080] Preferably, the common components of liquid microbial culture media include the following:

[0081] Carbon source: glucose, sodium acetate, methanol, methane saturated solution for methanogenic bacteria; nitrogen source: ammonium chloride, sodium nitrate, yeast extract; trace elements: iron, manganese, cobalt and other metal ion solutions to meet the metabolic needs of microorganisms in the extreme environment of the deep sea.

[0082] Applicable to: deep-sea bacteria and archaea, such as chemosynthetic bacteria in hydrothermal vents and methanogenic bacteria in cold seeps. It provides energy and substances needed for microbial growth and reproduction, and regulates their metabolic rate, such as by adjusting the carbon-nitrogen ratio to study growth curves.

[0083] Preferably, common components of algae culture media include the following:

[0084] Seawater solution containing nitrates, phosphates, vitamin B12, and trace elements such as copper and zinc.

[0085] Applicable to: deep-sea planktonic algae and coral symbiotic algae; promotes the growth of photosynthetic algae; used to study the photosynthetic efficiency or symbiotic relationship of algae in the abyssal layer under weak light conditions in the deep sea.

[0086] The other end of the gas supply pipe 9 is connected to the gas supply pipe 18. The gas supply pipe 18 is equipped with a gas concentration detector 10 and an electric air control valve 19. The gas supply pipe 18 is connected to the first culture tank 13, the second culture tank 14 and the third culture tank 15 respectively.

[0087] Specifically, the electric air control valve 19 can control whether gas is input into the first culture tank 13, the second culture tank 14, and the third culture tank 15.

[0088] Specifically, exposed components such as the protective shell 1, water inlet 3, baffle 6, protective plate 28, and the first culture tank 13, second culture tank 14, and third culture tank 15 are all coated with an anti-corrosion and wear-resistant coating; preferably, a polytetrafluoroethylene (PTFE) coating, a ceramic matrix composite coating, or a diamond-like carbon (DLC) coating material is used. Recovery can be achieved through deep-sea operations using submersibles such as ROVs or manned submersibles.

[0089] Furthermore, after the culture cycle is completed, all power equipment (oxygen generator, lighting, etc.) is turned off, the drain valve 24 of each culture tank is opened to discharge the waste liquid; the final sample status image is collected through the monitor 25;

[0090] Furthermore, the first culture tank 13, the second culture tank 14, and the third culture tank 15 are all equipped with sensors 7, which work with the monitor 25 to record the final environmental parameters.

[0091] Specifically, after the device is separated from the anchor, it is floated to the sea surface for recovery; after recovery, biological samples or sediments can be taken out of the culture tank (retained through filter screen 27);

[0092] Each of the first culture tank 13, the second culture tank 14, and the third culture tank 15 is equipped with a sealed hatch on one side, which facilitates the removal of biological samples or sediments from the culture tanks for subsequent analysis in conjunction with monitoring data. Specifically, the hatch uses conventional sealing methods in existing technology, such as a pressure-resistant sealing structure (e.g., a metal C-ring with an electric locking device), to ensure airtightness under the high pressure environment of the deep sea.

[0093] After the experiment, when the device is returned to the deck or laboratory, the operator can easily remove the biological samples (such as microbial colonies, algal communities, and small benthic organisms), sediment samples (retained by the filter 27 of the third culture tank 15), and culture medium from the culture tank by opening the sealed door. Combined with the image data recorded by the monitor 25 and the environmental parameters collected by the sensor 7, subsequent molecular biological analysis (such as gene sequencing), morphological observation (such as microscopic examination), or metabolite detection can be carried out, which fully connects the in-situ culture and laboratory analysis links and improves the completeness and accuracy of experimental data.

[0094] The system comprises three independent culture units: a first culture tank, a second culture tank, and a third culture tank. These units enable parallel, multi-dimensional experiments. The first culture tank creates a sealed, dark environment, suitable for studying the metabolic characteristics of anaerobic microorganisms or light-avoiding organisms. The second culture tank is equipped with adjustable-power LED lighting, simulating different deep-sea light gradients (e.g., weak light environments at depths of 200-1000 meters), allowing for targeted investigation of the photoadaptation mechanisms and symbiotic relationships of photosynthetic algae. The third culture tank, with its built-in filter, achieves stratified isolation between sediment and water, allowing focus on microscopic processes such as material exchange and community interactions at the benthic organism-sediment interface. Its differentiated design supports the simultaneous culture of different species (e.g., chemosynthetic bacteria, coral symbiotic algae, and amphipods) and allows for comparative analysis of the physiological responses of the same species under varying light intensity, nutrient ratios, and gas composition.

[0095] It should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This way of describing the specification is only for clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

[0096] The detailed descriptions listed above are merely specific descriptions of feasible embodiments of the present invention, and are not intended to limit the scope of protection of the present invention. All equivalent embodiments or modifications made without departing from the spirit of the present invention should be included within the scope of protection of the present invention.

Claims

1. A deep-sea in-situ culture device, comprising a protective shell (1), characterized in that, The protective shell (1) has a cavity (2), and the cavity (2) has a water inlet (3) and a gas delivery mechanism.

2. The deep-sea in-situ culture device according to claim 1, characterized in that, The cavity (2) is provided with a limiting block (4).

3. The deep-sea in-situ culture device according to claim 2, characterized in that, The cavity (2) is equipped with a sensor (7).

4. The deep-sea in-situ culture device according to claim 1, characterized in that, The protective shell (1) is equipped with an electric cylinder (5), which is connected to a baffle (6) via a piston rod.

5. The deep-sea in-situ culture device according to claim 1, characterized in that, The gas delivery mechanism includes an outlet pipe (12), a pressure swing adsorption oxygen generator (8) is connected to the protective shell (1), a gas delivery pipe (9) is connected to the pressure swing adsorption oxygen generator (8), a gas concentration detector (10) and an exhaust pipe (11) are connected to the gas delivery pipe (9), and an outlet pipe (12) is provided on the exhaust pipe (11).

6. The deep-sea in-situ culture device according to claim 1, characterized in that, The protective shell (1) is also provided with a protective plate (28) and a culture mechanism, the culture mechanism including a first culture tank (13), a second culture tank (14) and a third culture tank (15).

7. The deep-sea in-situ culture device according to claim 6, characterized in that, The first culture tank (13), the second culture tank (14) and the third culture tank (15) are all equipped with a drain valve (24) and a monitor (25); The second culture vessel (14) is also equipped with a lighting lamp (26); The third culture tank (15) is also equipped with a filter screen (27).

8. The deep-sea in-situ culture device according to any one of claims 1-3, characterized in that, The cavity (2) is provided with a water inlet pipe (16), and the water inlet pipe (16) is provided with at least three sets of electric valves (17). The water inlet pipe (16) is connected to the first culture tank (13), the second culture tank (14) and the third culture tank (15) respectively.

9. The deep-sea in-situ culture device according to claim 1, characterized in that, The protective shell (1) is provided with a liquid storage tank (20), the liquid storage tank (20) is provided with an inlet (21) and an inlet pipe (22), the inlet pipe (22) is provided with an electric valve (23), and the inlet pipe (22) is connected to a first culture tank (13), a second culture tank (14) or a third culture tank (15).

10. The deep-sea in-situ culture device according to claim 5, characterized in that, The other end of the gas supply pipe 1 (9) is connected to the gas supply pipe 2 (18). The gas supply pipe 2 (18) is equipped with a gas concentration detector (10) and an electric air control valve (19). The gas supply pipe 2 (18) is connected to the first culture tank (13), the second culture tank (14) and the third culture tank (15) respectively.

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