Microbial compound fertilizer and application thereof in perennial rice planting

Abstract

The invention discloses a microbial compound fertilizer and application thereof in perennial rice planting, and belongs to the technical field of microbial compound fertilizers. The microbial compound fertilizer is prepared from the following raw materials: a compound microbial flora consisting of brevibacillus laterosporus, pseudomonas fluorescens, azospirillum brasilense, soil bacillus and paecilomyces lilacinus. The microbial compound fertilizer provided by the invention can significantly improve the yield of perennial rice and soil health through organic-inorganic nutrient coordination and functional microbial community regulation and control, and is suitable for a perennial rice continuous cropping planting scene. The microbial compound fertilizer provided by the invention solves the problems of soil nutrient imbalance, root development limitation and the like in the planting process of perennial rice, and provides technical support for further improving the yield and production benefit of perennial rice.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of microbial compound fertilizer, in particular to a microbial compound fertilizer and application thereof in perennial rice planting. BACKGROUND

[0002] Perennial rice refers to a new type of rice variety that can be harvested for multiple years (seasons) after planting once. Compared with annual rice, perennial rice does not need to buy seeds, raise seedlings, plow and harrow fields, and transplant, etc. during the production process from the second season, and only needs to perform field management such as watering and fertilizing, and spraying pesticides to obtain considerable yield. Perennial rice can reduce labor input and production cost and improve rice production efficiency by simplifying rice planting links under the premise of basically unchanged yield compared with traditional rice, and is a new type of green and simplified rice production mode.

[0003] However, although perennial rice technology has advantages such as no-tillage and less tillage, it cannot be plowed and loosen soil due to continuous planting and no-tillage and no-seeding, and faces problems such as soil nutrient imbalance, limited root development, and decreased disease resistance during the planting process of perennial rice. Although traditional chemical fertilizer application can provide nutrients in the short term, long-term use can easily lead to lack of soil organic matter and humus, destruction of soil aggregate structure, soil acidification and hardening, and further cause yield reduction of perennial rice. Although microbial fertilizer can improve soil microecology, its fertilizer efficiency is relatively slow and difficult to meet the demand of rapid growth and high yield of perennial rice. At the same time, single use of microbial fertilizer can lead to incomplete nutrient supply and affect the overall nutrient absorption of perennial rice. Therefore, it is necessary to develop a microbial compound fertilizer that can improve soil microecology and provide comprehensive and rapid nutrients to better adapt to the planting demand of perennial rice and solve the problems such as soil nutrient imbalance and limited root development during the planting process of perennial rice, and further improve the yield and production efficiency of perennial rice. SUMMARY

[0004] The purpose of the present application is to provide a microbial compound fertilizer and application thereof in perennial rice planting to solve the problems existing in the prior art. The microbial compound fertilizer provided by the present application solves the problems such as soil nutrient imbalance and limited root development during the planting process of perennial rice and provides technical support for further improving the yield and production efficiency of perennial rice.

[0005] To achieve the above purpose, the present application provides the following solutions.

[0006] The present application provides a microbial compound fertilizer, the raw material composition of which comprises a compound microbial flora composed of Brevibacillus parvus, Pseudomonas fluorescens, Azospirillum brasilense, Brevibacillus agri, and Paecilomyces lilacinus.

[0007] Further, the complex microbial flora is used as raw material in the form of complex microbial bacterial liquid.

[0008] Further, the preparation method of the microbial bacterial liquid comprises the following steps:

[0009] Seed liquids of Brevibacillus laterosporus, Pseudomonas fluorescens, Azospirillum brasilense and Geobacillus terrae are prepared respectively and mixed, and a spore suspension of Paecilomyces lilacinus is added to the mixed bacterial liquid;

[0010] The effective viable cell number ratio of Brevibacillus laterosporus, Pseudomonas fluorescens, Azospirillum brasilense and Geobacillus terrae in the mixed bacterial liquid is (9-10):(8-10):(6-8):7;

[0011] The volume ratio of the mixed bacterial liquid to the spore suspension of Paecilomyces lilacinus is (7-10):(2-3);

[0012] The effective viable cell number in the mixed bacterial liquid is 1.0*10 9 cfu / mL, and the spore concentration of Paecilomyces lilacinus in the spore suspension is 1.0*10 6 cfu / mL.

[0013] Further, the raw material composition further comprises rotten straw residue, earthworm manure, ammonium nitrate, dipotassium hydrogen phosphate, magnesium sulfate, seaweed extract, humic acid, amino acid chelated zinc and potassium silicate.

[0014] Further, the raw material composition comprises the following components in parts by weight: 25-30 parts of rotten straw residue, 15-20 parts of earthworm manure, 8-10 parts of ammonium nitrate, 7-9 parts of dipotassium hydrogen phosphate, 4-6 parts of magnesium sulfate, 6-8 parts of seaweed extract, 5-7 parts of humic acid, 1-2 parts of amino acid chelated zinc, 0.5-1 part of potassium silicate, 4.5-5.5 parts of complex microbial bacterial liquid.

[0015] The application further provides application of the microbial compound fertilizer in improving the yield of perennial rice.

[0016] The application further provides application of the microbial compound fertilizer in improving the physical and chemical properties of the soil for planting perennial rice.

[0017] The application discloses the following technical effects:

[0018] Compared with the prior art, the microbial compound fertilizer provided by the present application can significantly improve the yield of perennial rice and soil health through the synergistic effect of organic-inorganic nutrients and the regulation of functional microbial community, and is suitable for perennial rice continuous planting scenarios. As nitrogen-fixing bacteria, Paenibacillus latericius and Azospirillum brasilense not only play a role in nitrogen fixation, but also provide carbon sources for phosphorus-solubilizing bacteria through the byproducts of nitrogen fixation. In addition, they can secrete growth hormones such as indole acetic acid and gibberellin to promote crop root development and improve the efficiency of soil nutrient absorption. As phosphorus-solubilizing bacteria, Pseudomonas fluorescens not only releases phosphorus to promote nitrogenase activity, but also inhibits diseases to some extent, thus creating a niche for potassium-dissolving bacteria. As potassium-dissolving bacteria, soil Bacillus releases potassium to promote the absorption of nitrogen and phosphorus by rice plants. The synergistic effect of multiple strains forms an efficient microbial ecosystem. This system not only enhances the biological activity of the soil, but also promotes the recycling of soil nutrients, providing a good soil environment for the growth of perennial rice. In addition, the organic components in the microbial compound fertilizer can improve soil structure, increase soil water and fertilizer retention capacity, and further improve the health of the soil.

[0019] The microbial compound fertilizer provided by the present application solves the problems of soil nutrient imbalance and limited root development during the planting of perennial rice, and provides technical support for further improving the yield and production efficiency of perennial rice. DETAILED DESCRIPTION

[0020] The various illustrative embodiments of the present application will now be described in detail below, which should not be considered as limiting the present application, but should be understood as a more detailed description of certain aspects, characteristics and embodiments of the present application.

[0021] It should be understood that the terms described in the present application are only for describing the specific embodiments, and are not used to limit the present application. In addition, for the numerical range in the present application, it should be understood that each intermediate value between the upper limit and the lower limit of the range is also specifically disclosed. Each smaller range between any stated value or intermediate value within the stated range, and any other stated value or intermediate value within the stated range, is also included in the present application. The upper limit and the lower limit of these smaller ranges can be independently included or excluded from the range.

[0022] Unless otherwise specified, all technical and scientific terms used herein have the same meaning as generally understood by those skilled in the art to which the present application pertains. Although preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can also be used in the implementation or testing of the present application. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials related to the documents. In the event of any conflict between the content of this specification and any incorporated document, the content of this specification shall prevail.

[0023] Many modifications and variations to the illustrative embodiments described herein will be apparent to those skilled in the art from consideration of the specification and practice of the subject technology. Additional embodiments of the technology will be apparent to those skilled in the art from consideration of the specification and practice of the subject technology. The specification and examples provided should be considered exemplary only.

[0024] As used herein, the terms "comprises," "comprising," "includes," "including," "has," "having," and the like are open-ended terms that are intended to mean including, but not limited to.

[0025] The present application provides a kind of microbial compound fertilizer suitable for perennial rice planting, raw material composition includes by Brevibacillus parvus, Pseudomonas fluorescens, Azospirillum brasilense, Bacillus agri and Paecilomyces lilacinus Compound microbial flora.

[0026] Optionally, the above-mentioned compound microbial flora is in the form of compound microbial liquid as raw material to participate in the preparation of microbial compound fertilizer.

[0027] Optionally, the preparation method of the above-mentioned compound microbial liquid comprises the following steps:

[0028] Respectively prepare seed liquid of Brevibacillus parvus, Pseudomonas fluorescens, Azospirillum brasilense and Bacillus agri and mix, add spore suspension of Paecilomyces lilacinus to mixed bacterial liquid;

[0029] The effective viable cell number ratio of Brevibacillus parvus, Pseudomonas fluorescens, Azospirillum brasilense and Bacillus agri in the above-mentioned mixed bacterial liquid is (9-10):(8-10):(6-8):7; preferably 10:10:8:7.

[0030] In the specific embodiments of the present application, the effective viable cell number in the above-mentioned mixed bacterial liquid is 1.0×10 9 cfu / mL, and the spore suspension concentration of the above-mentioned Paecilomyces lilacinus is 1.0×10 6 The mixed bacterial liquid and the spore suspension of Paecilomyces lilacinus are mixed in a volume ratio of (7-10):(2-3) to prepare the above-mentioned compound microbial liquid.

[0031] The above-mentioned microbial compound fertilizer also includes organic materials and inorganic compounds.

[0032] In the specific embodiments of the present application, the above-mentioned organic materials include rotten straw residue, earthworm manure, seaweed extract, humic acid and amino acid chelated zinc; and the above-mentioned inorganic compounds include ammonium nitrate, dipotassium hydrogen phosphate, magnesium sulfate and potassium silicate.

[0033] Optionally, the raw material composition of the microbial compound fertilizer by weight comprises the following components: 25-30 parts of mature straw residue, 15-20 parts of earthworm manure, 8-10 parts of ammonium nitrate, 7-9 parts of di-potassium hydrogen phosphate, 4-6 parts of magnesium sulfate, 6-8 parts of seaweed extract, 5-7 parts of humic acid, 1-2 parts of amino acid chelated zinc, 0.5-1 part of potassium silicate, and 4.5-5.5 parts of compound microbial liquid.

[0034] In the specific embodiments of the present application, the perennial rice test varieties are "Yunda 25" and "SPR1026", which are provided by the Guangxi Zhuang Autonomous Region Academy of Agricultural Sciences;

[0035] In the specific embodiments of the present application, the strains used are as follows:

[0036] Brevibacillus laterosporus, strain number: CICC 24775; Paecilomyces lilacinus, strain number: CICC 40276; Trichoderma harzianum, strain number: CICC 13056; Pseudomonas fluorescens, strain number: CICC 23919, all of the above strains are disclosed in the China Industrial Microbial Strain Preservation and Management Center, and the public can obtain them from the above-mentioned institution through a conventional route;

[0037] Azospirillum brasilense, strain number: CGMCC 1.5806; Bacillus soli, strain number: CGMCC 1.8813, all of the above strains are disclosed in the China General Microbial Strain Preservation and Management Center, and the public can obtain them from the above-mentioned institution through a conventional route.

[0038] The above strains are previously activated, expanded and cultured to prepare seed liquid or spore suspension, and are formulated into the required concentration according to the needs of the embodiments.

[0039] Example 1

[0040] The raw material composition of the microbial compound fertilizer by weight is as follows:

[0041] 25 parts of mature straw residue, 20 parts of earthworm manure, 10 parts of ammonium nitrate, 7 parts of di-potassium hydrogen phosphate, 4 parts of magnesium sulfate, 6 parts of seaweed extract, 5 parts of humic acid, 1.5 parts of amino acid chelated zinc, 1 part of potassium silicate, and 4.5 parts of compound microbial liquid.

[0042] The rotten straw residue has a water content of 30%, and is crushed to a particle size of 4 mm to improve mixing uniformity. The worm manure is completely mature worm manure, has no peculiar smell, is rich in organic matter and beneficial bacteria, and is sieved after air drying to remove large-particle impurities.

[0043] The preparation method of the complex microbial bacteria liquid is as follows:

[0044] The seed liquid of Bacillus laterosporus (1.0 x 10 9 cfu / mL), Pseudomonas fluorescens (1.0 x 10 9 cfu / mL), Azospirillum brasilense (1.0 x 10 9 cfu / mL), and Paenibacillus polymyxa (1.0 x 10 9 cfu / mL) is mixed in a volume ratio of 10:10:8:7 to obtain a mixed bacteria liquid; and the mixed bacteria liquid is mixed with a spore suspension (1.0 x 10 6 spores / mL) of Paecilomyces lilacinus in a volume ratio of 7:2 to obtain a complex microbial bacteria liquid.

[0045] Preparation steps of the microbial compound fertilizer:

[0046] The rotten straw residue, worm manure, ammonium nitrate, dipotassium hydrogen phosphate, magnesium sulfate, seaweed extract, humic acid, amino acid chelated zinc, and potassium silicate are accurately weighed according to the proportions. The above-mentioned solid raw materials are added into a horizontal stirrer, and stirred at a low speed for 10-15 min to ensure uniform mixing. After mixing, the complex microbial bacteria liquid is uniformly sprayed on the surface of the material in a spraying manner, and stirring is maintained during spraying to prevent local concentration from being too high or caking.

[0047] The mixed material is made into particles with a diameter of about 2-4 mm using a disc granulator, and the particles are sent into a fluidized bed dryer, which is controlled at a temperature of 40-50℃, and dried to a moisture content of ≤10%. The dried particles are naturally cooled to room temperature.

[0048] Example 2

[0049] The raw material composition of the microbial compound fertilizer is as follows in terms of weight parts:

[0050] The rotten straw residue is 30 parts, the worm manure is 15 parts, the ammonium nitrate is 8 parts, the dipotassium hydrogen phosphate is 9 parts, the magnesium sulfate is 6 parts, the seaweed extract is 8 parts, the humic acid is 7 parts, the amino acid chelated zinc is 2 parts, the potassium silicate is 0.5 parts, and the complex microbial bacteria liquid is 5.5 parts.

[0051] In the above-mentioned raw materials, the rotten straw residue has a water content of 25%, and is crushed to a particle size of 3 mm to improve mixing uniformity. The worm manure is completely mature worm manure, has no peculiar smell, is rich in organic matter and beneficial bacteria, and is sieved after air drying to remove large-particle impurities.

[0052] The preparation method of the complex microbial bacteria liquid is as follows:

[0053] Brevibacillus laterosporus (1.0 x 10 9 cfu / mL), Pseudomonas fluorescens (1.0 x 10 9 cfu / mL), Azospirillum brasilense (1.0 x 10 9 cfu / mL), and Bacillus agri (1.0 x 10 9 cfu / mL) were mixed in a volume ratio of 10:8:6:7 to obtain a mixed bacterial solution; and the mixed bacterial solution was mixed with a spore suspension of P. lilacinum (1.0 x 10 6 spores / mL) in a volume ratio of 8:3 to obtain a compound microbial bacterial solution.

[0054] Microbial compound fertilizer preparation steps:

[0055] The rotten straw residues, earthworm manure, ammonium nitrate, dipotassium hydrogen phosphate, magnesium sulfate, seaweed extract, humic acid, amino acid chelated zinc, and potassium silicate were accurately weighed according to the proportions. The above-mentioned solid raw materials were added into a horizontal stirrer, and stirred at a low speed for 10-15 min to ensure uniform mixing. After mixing, the compound microbial bacterial solution was uniformly sprayed on the surface of the material in a spraying manner, and stirring was maintained during spraying to prevent local concentration from being too high or caking.

[0056] The mixed material was made into particles with a diameter of about 2-4 mm using a disc granulator, and the particles were sent into a fluidized bed dryer, which was controlled at a temperature of 40-50℃, and dried to a moisture content of ≤10%. The dried particles were naturally cooled to room temperature.

[0057] Example 3

[0058] The raw material composition of the microbial compound fertilizer was as follows in terms of weight parts:

[0059] Rotten straw residues 28 parts, earthworm manure 18 parts, ammonium nitrate 8 parts, dipotassium hydrogen phosphate 8 parts, magnesium sulfate 5 parts, seaweed extract 7 parts, humic acid 6 parts, amino acid chelated zinc 1 part, potassium silicate 1 part, and compound microbial bacterial solution 5 parts.

[0060] In the above-mentioned raw materials, the rotten straw residues had a water content of 28%, and were crushed to a particle size of 3 mm to improve mixing uniformity; the earthworm manure was completely matured earthworm manure without odor, rich in organic matter and beneficial bacteria, and was sieved after air-drying to remove large-particle impurities.

[0061] The preparation method of the compound microbial bacterial solution was as follows:

[0062] Brevibacillus laterosporus (1.0 x 10 9 cfu / mL), Pseudomonas fluorescens (1.0 x 10 9 cfu / mL), Azospirillum brasilense (1.0 x 10 9CFU / mL) and soil Bacillus (1.0 × 10⁻⁶) 9 The seed culture (cfu / mL) was mixed at a volume ratio of 9:10:7:7 to obtain a mixed bacterial culture; this was then mixed with a spore suspension of *Paecilomyces lilacinus* (1.0 × 10⁻⁶ CFU / mL). 6 Mix (number of cells / mL) at a volume ratio of 10:3 to obtain a composite microbial culture.

[0063] Preparation steps of microbial compound fertilizer:

[0064] Accurately weigh the following ingredients according to the specified proportions: decomposed straw residue, earthworm castings, ammonium nitrate, dipotassium hydrogen phosphate, magnesium sulfate, seaweed extract, humic acid, amino acid chelated zinc, and potassium silicate. Add the solid materials to a horizontal mixer and mix at low speed for 10–15 minutes to ensure uniform mixing. After mixing, spray the compound microbial inoculum evenly onto the surface of the materials using a sprayer, maintaining stirring during spraying to prevent excessively high local concentrations or clumping.

[0065] The mixture is granulated into particles with a diameter of approximately 2–4 mm using a disc granulator. The particles are then fed into a fluidized bed dryer, where the temperature is controlled between 40 and 50°C, until the moisture content is ≤10%. The dried particles are then allowed to cool naturally to room temperature.

[0066] Comparative Example 1

[0067] Compared with Example 1, the raw material composition of the microbial compound fertilizer does not contain compound microbial liquid, but the other raw materials and preparation methods are the same.

[0068] Comparative Example 2

[0069] Compared with Example 1, the method for preparing the composite microbial inoculum is as follows:

[0070] Lateral spores (1.0 × 10⁻⁶) 9 CFU / mL), Fluorescent Pseudomonas (1.0 × 10⁻⁶) 9 cfu / mL), Azotobacter brasiliensis (1.0×10⁻⁶) 9 CFU / mL) and soil Bacillus (1.0 × 10⁻⁶) 9 Seed culture of *Paecilomyces lilacinus* (cfu / mL) was mixed at a volume ratio of 10:10:8:7 to obtain mixed bacterial culture A; *Paecilomyces lilacinus* (1.0 × 10⁻⁶ CFU / mL) was added. 6 (cells / mL) and Trichoderma harzianum (1.0×10⁻⁶) 6 Spore suspensions (spores / mL) were mixed at a volume ratio of 1:1 to obtain mixed bacterial solution B. Mixed bacterial solution A and mixed bacterial solution B were then mixed at a volume ratio of 7:2 to obtain composite microbial solution.

[0071] The other raw materials and preparation methods are the same.

[0072] Comparative Example 3

[0073] Compared with Example 1, the composite microbial liquid does not contain P. lilacinum, and other raw materials and preparation methods are the same.

[0074] Field test

[0075] Test design

[0076] A test field is set up in a perennial rice planting area, and the test area infrastructure is complete, with good irrigation and drainage capacity. The physicochemical properties of the soil in the test area are: organic matter 22.4 g / kg, total nitrogen 1.23 g / kg, available phosphorus 7.2 mg / kg, available potassium 38.7 mg / kg, and pH 5.3. The test rice varieties are "Yundai 25" and "SPR1026". The planting density is 253,751 holes / hm 2 . The fertilization method is: base application, 78 kg / hm 2 ; and tillering stage topdressing, 25 kg / hm 2 . The test has 6 treatments, 3 repetitions, and the same management measures except for different types of compound fertilizers.

[0077] After the rice matures, 5 plants are selected from each test plot for investigation, and the plant height, effective tiller number, and thousand-grain weight are recorded, and the actual yield of each test plot, i.e., the weight of rice (excluding four peripheral rows, empty stalks, and disease and pest variant plants), is tested. The results are shown in Table 1.

[0078] After two years of planting, 5 soil samples are randomly collected from each test plot, uniformly mixed, and sampled by the four-part method. After the soil samples are collected, they are air-dried and sieved for determination of physicochemical properties. Soil organic matter is determined by potassium dichromate-sulfuric acid oxidation method. Soil total nitrogen is determined by semi-micro Kjeldahl nitrogen determination method. Available phosphorus is determined by colorimetric method after soil samples are extracted with hydrochloric acid-ammonium fluoride at a solid-liquid ratio of 1:10 (g / mL). Available potassium is determined by flame photometry after soil samples are extracted with 1 mol / L ammonium acetate at a solid-liquid ratio of 1:10 (g / mL). The results are shown in Table 2.

[0079] Table 1 Growth and yield indicators

[0080]

[0081]

[0082] Table 2 Soil physicochemical properties (after 2 years of planting)

[0083] Treatment Organic matter (g / kg) Total nitrogen (g / kg) Available phosphorus (g / kg) Available potassium (g / kg) Example 1 28.7 1.56 11.1 53.6 Example 2 27.9 1.52 10.8 52.4 Example 3 28.3 1.54 10.9 52.8 Comparative Example 1 19.8 1.15 7.0 38.1 Comparative Example 2 24.5 1.38 9.5 48.5 Comparative Example 3 22.8 1.31 8.6 44.3

[0084] Result analysis

[0085] As can be seen from Table 1, Examples 1-3 are superior to Comparative Examples 1-3 in terms of plant height, effective tiller number, thousand-grain weight and actual yield, indicating that the synergistic effect of the microbial flora and organic-inorganic components can significantly promote the vertical growth of perennial rice. The soil organic matter and nutrient content shows that the treatments of Examples 1-3 can effectively improve the fertility of the continuous cropping soil, among which the organic matter content of Example 1 is most obviously improved.

[0086] In summary, the microbial compound fertilizer provided by the present application can significantly improve the yield of perennial rice and soil health through the synergistic effect of organic-inorganic nutrients and functional microbial community regulation, and is suitable for perennial rice continuous cropping planting scenarios. As nitrogen-fixing bacteria, Paenibacillus latericius and Azospirillum brasilense not only play a role in nitrogen fixation, but also provide carbon sources for phosphorus-dissolving bacteria through the by-products of nitrogen fixation. At the same time, they can also secrete growth hormones such as indole acetic acid and gibberellin to promote crop root development and improve the efficiency of soil nutrient absorption. As phosphorus-dissolving bacteria, Pseudomonas fluorescens not only releases phosphorus to promote nitrogenase activity, but also inhibits diseases to some extent, creating a niche for potassium-dissolving bacteria. As potassium-dissolving bacteria, soil Bacillus releases potassium to promote the absorption of nitrogen and phosphorus by rice plants. The synergistic effect of multiple strains forms an efficient microbial ecosystem. This system not only enhances the biological activity of the soil, but also promotes the recycling of soil nutrients, providing a good soil environment for the growth of perennial rice. In addition, the organic components in the microbial compound fertilizer can improve the soil structure, increase the soil water and fertilizer retention capacity, and further improve the health status of the soil.

[0087] The above-described examples are only preferred modes of the present application and do not limit the scope of the present application. Without departing from the design spirit of the present application, various modifications and improvements to the technical solutions of the present application made by those of ordinary skill in the art should fall within the scope of protection determined by the claims of the present application.

Claims

1. A microbial compound fertilizer, characterized in that, The raw material composition includes a complex microbial community consisting of Bacillus lateralis, Pseudomonas fluorescens, Azotobacter brasiliensis, Bacillus sphaeroides, and Paecilomyces lilacinus.

2. The microbial compound fertilizer according to claim 1, characterized in that, The composite microbial community is used as a raw material in the form of composite microbial liquid.

3. The microbial compound fertilizer according to claim 2, characterized in that, The method for preparing the microbial culture includes the following steps: Seed cultures of Bacillus lateralis, Pseudomonas fluorescens, Azotobacter brasiliensis and Bacillus stolonifer were prepared separately and mixed. A spore suspension of Paecilomyces lilacinus was added to the mixed culture. The ratio of viable counts of Bacillus laterosporus, Pseudomonas fluorescens, Azotobacter brasiliensis, and Bacillus agronomycetes in the mixed bacterial solution is (9-10):(8-10):(6-8):7; The volume ratio of the mixed bacterial solution to the spore suspension of Paecilomyces lilacinus is (7-10):(2-3); The effective viable count in the mixed bacterial solution is 1.0 × 10⁻⁶. 9 The cfu / mL concentration of Paecilomyces lilacinus spores in the spore suspension was 1.0 × 10⁻⁶. 6 per mL.

4. The microbial compound fertilizer according to claim 2, characterized in that, The raw material composition also includes decomposed straw residue, earthworm castings, ammonium nitrate, dipotassium hydrogen phosphate, magnesium sulfate, seaweed extract, humic acid, amino acid chelated zinc, and potassium silicate.

5. The microbial compound fertilizer according to claim 4, characterized in that, By weight, the raw material composition includes the following components: 25-30 parts of decomposed straw residue, 15-20 parts of earthworm castings, 8-10 parts of ammonium nitrate, 7-9 parts of dipotassium hydrogen phosphate, 4-6 parts of magnesium sulfate, 6-8 parts of seaweed extract, 5-7 parts of humic acid, 1-2 parts of amino acid chelated zinc, 0.5-1 part of potassium silicate, and 4.5-5.5 parts of compound microbial inoculum.

6. The application of the microbial compound fertilizer according to any one of claims 1-5 in increasing the yield of perennial rice.

7. The application of the microbial compound fertilizer according to any one of claims 1-5 in improving the physicochemical properties of soil for perennial rice cultivation.

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