Fermentation tank for biotechnology and fermentation method
By installing a defoaming component in the fermenter, including a crushing, adding, and dispensing structure, the problem of a single defoaming plate being unable to handle a large amount of foam is solved, achieving uniform distribution of the defoamer and effective removal of bubbles, thus improving the defoaming effect.
Patent Information
- Application Number
- CN202511206554.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-08-27
- Publication Date
- 2025-11-21
AI Technical Summary
In existing fermenters, a single defoaming plate is insufficient to effectively handle situations with excessive foam. Too high a rotation speed affects the product inside the fermenter, while too low a rotation speed results in poor defoaming effect. Furthermore, when the defoaming plate rotates, the foam is easily thrown into the fermenter, making cleaning difficult.
A biotechnology fermenter includes a tank body with a cover plate fixed to the top. A drive motor is fixed to the center of the top of the cover plate. The bottom of a rotating rod extends into the tank body, and a stirring rod is fixed to the outer wall of the rotating rod. A defoaming component is installed on the stirring rod. The defoaming component includes a breaking structure, an adding structure, and a dispensing structure. The breaking structure is used to separate the foam, the adding structure is used to add defoamer, and the dispensing structure is used to evenly dispense the defoamer.
The defoaming board effectively breaks down larger foams by rotating the lever to drive the breaking structure. The addition and delivery structures ensure that the defoamer is evenly distributed, and the defoaming components prevent bubbles from adhering. This solves the problem that a single defoaming board is difficult to deal with when there is a lot of foam, thus improving the defoaming effect.
Smart Images
Figure CN120988809A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of fermentation technology, and more particularly to a fermentation tank and fermentation method for biotechnology. Background Technology
[0002] A fermenter is a bioreactor used for the culture of microorganisms or cells. It is widely used in pharmaceuticals, food, chemicals, environmental protection, and bioenergy. By precisely controlling parameters such as temperature, pH, dissolved oxygen, stirring, and aeration, a fermenter provides the optimal growth environment for microorganisms or cells to achieve efficient production of target products.
[0003] In existing technologies, foam is generated inside fermenters. Fermenters usually break up the foam by using defoaming plates installed inside. However, a single defoaming plate is insufficient to handle large amounts of foam. If the rotation speed is too fast, it can easily affect the product inside the fermenter. If the defoaming plate rotates too slowly, the defoaming effect will be minimal. At the same time, the defoaming plate can also cause foam to be thrown into the fermenter when it rotates, which is not easy to clean. Therefore, this application proposes a fermenter and fermentation method for biotechnology. Summary of the Invention
[0004] The purpose of this invention is to address the problem that a single defoaming board in the prior art is insufficient to handle excessive foam, and to propose a biotechnology fermenter and fermentation method.
[0005] In a first aspect, the present invention proposes a fermenter for biotechnology, comprising a tank body, a cover plate fixedly connected to the top of the tank body, a drive motor fixedly connected to the center of the top of the cover plate, a rotating rod fixedly connected to the output end of the drive motor, the bottom of the rotating rod extending into the tank body, a stirring rod fixedly connected to the outer wall of the rotating rod, and a defoaming component for eliminating foam provided on the outer wall of the rotating rod, the defoaming component comprising a crushing structure, an adding structure, and a feeding structure; The breaking structure is used to divide the foam and cause the foam to break up. The breaking structure includes a defoaming plate, which is installed on the rotating rod. The addition structure is used to add defoamer into the defoaming plate; The delivery structure is used to uniformly deliver the defoamer inside the defoaming board, and the delivery structure is disposed on the defoaming board. It also includes a defoaming assembly for eliminating air bubbles adhering to the stirring rod.
[0006] Optionally, the crushing structure further includes a collar, which is sleeved on the outer wall of the rotating rod. The defoaming plate is fixed to one side of the collar, and the bottom of the defoaming plate is provided with multiple sets of scraping teeth. The outer wall of the rotating rod is provided with a groove, and the inner wall of the collar is fixed with a protrusion. The protrusion is slidably connected inside the groove. The outer wall of the rotating rod is equipped with two sets of circular plates, which are respectively installed at the top and bottom of the groove. The end of the defoaming plate away from the collar is fixed with a float. There are four sets of both the defoaming plate and the float, which are circumferentially distributed on the outer wall of the collar.
[0007] Optionally, the added structure includes a sterile filter fixed to the outer wall of the rotating rod and positioned at the top of the cover plate. A flow groove is formed on the inner wall of the rotating rod, and a connecting pipe is fixed to the outer wall of the rotating rod at the bottom of the cover plate. The connecting pipe is connected to the sterile filter through the flow groove. A telescopic tube is fixed to the bottom of the connecting pipe, and the bottom of the telescopic tube is fixed to the top of the defoaming plate. A storage groove is formed inside the defoaming plate, and a connecting ring is fixed between the four sets of defoaming plates. A connecting groove is formed inside the connecting ring, and the four sets of storage grooves are connected through the connecting groove.
[0008] Optionally, the delivery structure includes a baffle plate disposed on one side of the defoaming plate, a guide groove provided on one side of the storage tank, the baffle plate disposed at the port of a set of guide grooves, a sealing gasket fixedly connected to one end of the baffle plate near the guide groove, an installation groove provided inside the defoaming plate, a first spring fixedly connected to the inner wall of the installation groove, a pull plate fixedly connected to one end of the first spring, a pull rod fixedly connected to one side of the pull plate, and one end of the pull rod extending out of the installation groove and fixedly connected to one side of the baffle plate.
[0009] Optionally, a mounting plate is fixed to one side of the float, and a scraper is fixed to one side of the mounting plate. The scraper is inclined and one side of the scraper is attached to the inner wall of the tank. A reinforcing block is fixed to one side of the scraper. The reinforcing block is triangular in shape, and both the scraper and the reinforcing block are made of rubber.
[0010] Optionally, the defoaming assembly includes a pusher plate disposed at the top of the stirring rod. The stirring rod has a slot inside, a second spring is fixedly connected to one side of the slot, a push plate is fixedly connected to one end of the second spring, a connecting block is fixedly connected to the top of the push plate, a square slot is disposed at the top of the stirring rod, the connecting block is slidably connected inside the square slot, a connecting rod is fixedly connected to the top of the connecting block, and the connecting rod is fixedly connected to one end of the pusher plate. Two sets of pusher plates are provided and symmetrically distributed at the top and bottom of the stirring rod, and the two sets of pusher plates are connected by a side rod.
[0011] Optionally, the square groove has sliding grooves on both sides, and the connecting block has sealing plates fixed to both sides, with the sealing plates slidably connected to the inner wall of the sliding groove.
[0012] Optionally, a cooling shell is fixed to the outer wall of the tank, a water inlet is fixed to one side of the cooling shell, a temperature sensor is fixed to one side of the cooling shell, a solenoid valve is fixed to the outer wall of the water inlet, the temperature sensor is connected to the solenoid valve via a wire, a detection probe is fixed to one end of the temperature sensor, one end of the detection probe extends into the tank, and a water outlet is fixed to the other side of the cooling shell.
[0013] Optionally, an arc-shaped plate is fixed to the inner wall of the tank. The arc-shaped plate is disposed between the upper and lower sets of stirring rods. There are three sets of arc-shaped plates, which are distributed circumferentially on the inner wall of the tank.
[0014] In a second aspect, the present invention provides a fermentation method for biotechnology, applied to the biotechnology fermenter described in the first aspect, the method comprising the following steps: S1: Clean the inside of the fermenter, remove the residue from the previous batch, and sterilize the inside of the fermenter with steam; S2: The culture medium is sterilized at high temperature and then inoculated into the culture medium. After inoculation, it is placed inside the tank and the culture medium is evenly mixed by stirring rod. S3: When there is a lot of foam, the drive motor drives the rotating rod and defoaming plate to rotate. When the defoaming plate rotates, it will break the foam through the convex teeth below, and in conjunction with the addition structure and the delivery structure, the defoamer will be evenly spread on the foam, causing the foam to disappear. S4: After air is added into the tank, the drive motor drives the rotating rod and stirring rod to rotate so that the air is evenly distributed, and the defoaming component eliminates the air bubbles attached to the stirring rod.
[0015] Compared with the prior art, this application includes at least one of the following beneficial technical effects: This invention utilizes a rotating rod to drive the breaking structure, facilitating the breaking down of larger foams via the defoaming plate. An addition structure allows for the addition of defoamer to the storage tank of the defoaming plate. A dispensing structure allows gravity to temporarily open the baffle when the defoaming plate rotates, releasing the defoamer from the storage tank. The rotation of the defoaming plate further promotes uniform mixing of the defoamer and foam, making it easier to remove scum. The defoaming component operates in conjunction with the rotating stirring rod, preventing bubbles from adhering to the stirring rod and solving the problem of a single defoaming plate being insufficient to handle large amounts of scum. Attached Figure Description
[0016] Figure 1A schematic diagram of the overall structure of a fermenter and fermentation method for biotechnology; Figure 2 This is a schematic cross-sectional view of a fermenter and fermentation method for biotechnology. Figure 3 for Figure 2 An enlarged structural diagram at point A; Figure 4 This is a three-dimensional schematic diagram of the broken structure; Figure 5 This is a schematic diagram of the cross-sectional structure of the defoaming board; Figure 6 for Figure 5 An enlarged structural diagram at point B; Figure 7 This is a schematic diagram of the scraper structure; Figure 8 This is a schematic diagram of the rotating rod and stirring rod structure; Figure 9 This is a schematic diagram of the cross-sectional structure of the stirring rod; Figure 10 for Figure 9 An enlarged structural diagram at point C; Figure 11 This is a schematic diagram of the pusher structure.
[0017] Reference numerals: 1. Tank body; 3. Cover plate; 5. Drive motor; 6. Rotating rod; 7. Stirring rod; 9. Collar; 10. Defoaming plate; 11. Float; 12. Groove; 13. Protrusion; 14. Circular plate; 15. Aseptic filter; 16. Flow channel; 17. Connecting pipe; 18. Telescopic pipe; 19. Storage tank; 20. Connecting ring; 21. Communicating channel; 22. Baffle; 23. Sealing gasket; 24. Pull rod; 25. Guide channel ; 26. Mounting slot; 27. First spring; 28. Pull plate; 29. Mounting plate; 30. Scraper; 31. Reinforcing block; 32. Push plate; 33. Empty slot; 34. Second spring; 35. Push plate; 36. Connecting block; 37. Sealing plate; 38. Slide groove; 39. Connecting rod; 40. Side rod; 41. Cooling shell; 42. Water inlet; 43. Water outlet; 44. Temperature sensor; 45. Detection probe; 46. Curved plate. Detailed Implementation
[0018] To make the objectives, features, and advantages of this invention more apparent and understandable, the technical solutions of the embodiments of this invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the embodiments described below are only some embodiments of this invention, and not all embodiments. Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this invention.
[0019] In the description of this invention, it should be understood that the terms "upper," "lower," "top," "bottom," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing the invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the invention. It should be noted that when a component is considered to be "connected" to another component, it can be directly connected to the other component or there may be a component positioned centrally in the connection.
[0020] The technical solution of the present invention will be further described below with reference to the accompanying drawings and specific embodiments.
[0021] like Figure 1 - Figure 8As shown, the present invention proposes a biotechnology fermenter, comprising a tank body 1, a cover plate 3 fixedly connected to the top of the tank body 1, a drive motor 5 fixedly connected to the center of the top of the cover plate 3, a rotating rod 6 fixedly connected to the output end of the drive motor 5, the bottom of the rotating rod 6 extending into the tank body 1, and a stirring rod 7 fixedly connected to the outer wall of the rotating rod 6. The invention is characterized in that: the outer wall of the rotating rod 6 is provided with a defoaming component for eliminating foam, the defoaming component including a crushing structure, an adding structure, and a feeding structure; the crushing structure is used to divide the foam, promoting its breakage; the crushing structure includes a defoaming plate 10, the defoaming plate 10 being mounted on the rotating rod 6; the crushing structure also includes a collar 9, the collar 9 being sleeved on the outer wall of the rotating rod 6; the defoaming... The defoaming plate 10 is fixed to one side of the collar 9. Multiple sets of scraping teeth are provided at the bottom of the defoaming plate 10. A groove 12 is formed on the outer wall of the rotating rod 6. A protrusion 13 is fixed to the inner wall of the collar 9, and the protrusion 13 is slidably connected inside the groove 12. Two sets of circular plates 14 are installed on the outer wall of the rotating rod 6, respectively installed at the top and bottom of the groove 12. A float 11 is fixed to the end of the defoaming plate 10 away from the collar 9. Four sets of both the defoaming plate 10 and the float 11 are provided, arranged circumferentially on the outer wall of the collar 9. The adding structure is used to add defoaming agent into the defoaming plate 10. The adding structure includes a sterile filter 15, which is fixed to the outer wall of the rotating rod 6. A flow groove 16 is formed on the inner wall of the rotating rod 6, which is located at the top of the cover plate 3. A connecting pipe 17 is fixed to the outer wall of the rotating rod 6. The connecting pipe 17 is located at the bottom of the cover plate 3 and is connected to the sterile filter 15 through the flow groove 16. A telescopic pipe 18 is fixed to the bottom of the connecting pipe 17 and is fixed to the top of the defoaming plate 10. A storage tank 19 is formed inside the defoaming plate 10. A connecting ring 20 is fixed between the four sets of defoaming plates 10. A connecting groove 21 is formed inside the connecting ring 20. The four sets of storage tanks 19 are connected through the connecting groove 21. The dispensing structure is used to evenly dispense the defoaming agent in the defoaming plate 10. The dispensing structure is set on the defoaming plate 10. The delivery structure includes a baffle 22 disposed on one side of the defoaming plate 10. A guide groove 25 is provided on one side of the storage tank 19. The baffle 22 is located at the port of a set of guide grooves 25. A sealing gasket 23 is fixedly connected to one end of the baffle 22 near the guide groove 25. An installation groove 26 is provided inside the defoaming plate 10. A first spring 27 is fixedly connected to the inner wall of the installation groove 26. A pull plate 28 is fixedly connected to one end of the first spring 27. A pull rod 24 is fixedly connected to one side of the pull plate 28. One end of the pull rod 24 extends out of the installation groove 26 and is fixedly connected to one side of the baffle 22. An installation plate 29 is fixedly connected to one side of the float 11. A scraper 30 is fixedly connected to one side of the installation plate 29. The scraper 30 is inclined.One side of the scraper 30 is attached to the inner wall of the tank 1. A reinforcing block 31 is fixedly connected to one side of the scraper 30. The reinforcing block 31 is triangular in shape. Both the scraper 30 and the reinforcing block 31 are made of rubber. An arc-shaped plate 46 is fixedly connected to the inner wall of the tank 1. The arc-shaped plate 46 is disposed between the upper and lower sets of stirring rods 7. Three sets of arc-shaped plates 46 are disposed and distributed circumferentially on the inner wall of the tank 1.
[0022] To address the issue of excessive foaming caused by a single defoaming plate 10, when fermentation is required, the material can be added to the tank 1. The drive motor 5 rotates the rotating rod 6 and stirring rod 7, causing the stirring rod 7 to agitate the material and promote fermentation. An arc-shaped plate 46 is installed inside the tank 1, which works in conjunction with the rotating rod 7 to ensure uniform mixing of the material. When excessive foam appears above the material, a float 11 at the end of the defoaming plate 10 allows it to float above the material, facilitating contact between the scraper teeth below the float 11 and the floating foam. As the water level changes, the heights of the float 11, defoaming plate 10, and collar 9 also adjust accordingly. The change is achieved by the engagement of the protrusion 13 and the groove 12, which keeps the collar 9 connected to the rotating rod 6. When the rotating rod 6 rotates, the collar 9 is engaged with the groove 12 by the protrusion 13, thus allowing the collar 9 to rotate with the rotating rod 6. Simultaneously, the rotation of the collar 9 drives the four sets of defoaming plates 10 to rotate together. The multiple sets of scraping teeth at the bottom of the defoaming plates 10 enhance the shearing force on the foam during rotation, facilitating the elimination of larger foam particles. When defoaming agent needs to be added to the storage tank 19 of the defoaming plates 10, it can be injected into the sterile filter 15, allowing the defoaming agent to pass through the filter and achieve a sterile state. The filtered defoaming agent then flows into the flow channel 16 and into… Within the connecting pipe 17 and the telescopic pipe 18, the telescopic pipe 18 ensures that the connection between the connecting pipe 17 and the storage tank 19 within the defoaming plate 10 remains maintained even when the height of the defoaming plate 10 changes. After the defoamer enters the storage tank 19 through the telescopic pipe 18, the defoamer in one set of storage tank 19 flows to the other three sets of storage tank 19 through the connecting groove 21 within the connecting ring 20, thus evenly distributing the defoamer within the storage tank 19 of the four sets of defoaming plates 10. When the defoaming plate 10 remains stationary, the pull plate 28 can be pushed to one side by the first spring 27. When the pull plate 28 is pushed, it will cause the pull rod 24 and the baffle 22 on one side to generate a pushing force to one side. This allows the baffle 22 and sealing gasket 23 to fit tightly against the port of the guide groove 25, preventing the defoamer from flowing out. When the defoamer plate 10 rotates, it drives the first spring 27, pull plate 28, pull rod 24, and baffle 22 to rotate together. Due to inertia, the pull plate 28, pull rod 24, and baffle 22 will generate a force opposite to that of the first spring 27, thus offsetting part of the spring's thrust and causing the pull plate 28, pull rod 24, and baffle 22 to move slightly to one side. When the baffle 22 moves away from the port of the guide groove 25, the defoamer in the storage tank 19 will flow outward through the guide groove 25. Since multiple sets of guide grooves 25 are arranged in parallel, the range of defoamer dispensing can be expanded. After the defoamer comes into contact with the foam, it helps to eliminate the foam.The continuous rotation of the defoaming plate 10 agitates the foam and defoamer with its scraper teeth at the bottom, ensuring better contact between the defoamer and the foam. The rotation of the defoaming plate 10 also rotates the float 11, mounting plate 29, and scraper 30. Since the scraper 30 is attached to the inner wall of the tank 1 and is angled, its rotation removes foam adhering to the inner wall of the tank 1. The angled shape of the scraper 30 guides the foam downwards, allowing it to continue floating on the material and preventing foam accumulation on the inner wall of the tank 1.
[0023] like Figure 8 - Figure 11 The defoaming assembly includes a pusher plate 32, which is disposed at the top of the stirring rod 7. The stirring rod 7 has a hollow groove 33 inside. A second spring 34 is fixedly connected to one side of the hollow groove 33. A pusher plate 35 is fixedly connected to one end of the second spring 34. A connecting block 36 is fixedly connected to the top of the pusher plate 35. A square groove is disposed at the top of the stirring rod 7. The connecting block 36 is slidably connected inside the square groove. A connecting rod 39 is fixedly connected to the top of the connecting block 36. The connecting rod 39 is fixedly connected to one end of the pusher plate 32. Two sets of pushers 32 are provided and are symmetrically distributed at the top and bottom of the stirring rod 7. The two sets of pushers 32 are connected by a side rod 40. Sliding grooves 38 are disposed on both sides of the square groove. Sealing plates 37 are fixedly connected to both sides of the connecting block 36. The sealing plates 37 are slidably connected to the inner wall of the sliding grooves 38.
[0024] After air is introduced into the tank 1, some air bubbles will adhere to the connection between the stirring rod 7 and the rotating rod 6. When the stirring rod 7 rotates, centrifugal force is generated, which causes the push plate 35 in the groove 33 of the stirring rod 7 to exert an outward force, thereby enabling the push plate 35 to squeeze the second spring 34 to one side. When the push plate 35 moves to one side, it also drives the connecting block 36, connecting rod 39, side rod 40 and two sets of push plates 32 to move to one side together. When the push plates 32 move, they can push the passing air bubbles to the surroundings, thereby preventing air bubbles from adhering to the outer wall of the stirring rod 7. When the stirring rod 7 stops rotating, the centrifugal force on the push plate 35, connecting block 36, connecting rod 39, side rod 40 and two sets of push plates 32 will disappear. At this time, the second spring 34 will be released. The restoring force of the spring 34 will push the push plate 35 to one side. When the push plate 35 moves, it will drive the connecting block 36, connecting rod 39, side rod 40 and two sets of push plates 32 to reset together. When the two sets of push plates 32 reset, the conical teeth on one side of the push plate 32 can puncture the air bubbles at the connection between the stirring rod 7 and the rotating rod 6, which can prevent air bubbles from adhering to the connection between the stirring rod 7 and the rotating rod 6. When the connecting block 36 moves, the remaining position of the square groove can be sealed by the two sets of sealing plates 37. By setting the sliding grooves 38 on both sides of the square groove, the two sets of sealing plates 37 can be stored inside the sliding grooves 38, so that when the connecting block 36 moves, the sealing plates 37 can keep the square groove sealed as the connecting block 36 moves.
[0025] like Figure 1 and Figure 2 A cooling shell 41 is fixed to the outer wall of the tank 1. A water inlet 42 is fixed to one side of the cooling shell 41. A temperature sensor 44 is fixed to one side of the cooling shell 41. A solenoid valve is fixed to the outer wall of the water inlet 42. The temperature sensor 44 is connected to the solenoid valve via a wire. A detection probe 45 is fixed to one end of the temperature sensor 44. One end of the detection probe 45 extends into the tank 1. A water outlet 43 is fixed to the other side of the cooling shell 41.
[0026] When the temperature inside the detection head tank 1 is high, the solenoid valve can be opened, and the cooling water can flow into the cooling shell 41 through the inlet 42 by opening the solenoid valve. The water is cooled by the heat conduction of the liquid, and the water that has absorbed heat is discharged through the outlet 43.
[0027] A fermentation method for biotechnology, comprising the following steps: S1: Clean the inside of the fermenter, remove the residue from the previous batch, and sterilize the inside of the fermenter with steam; S2: The culture medium is sterilized at high temperature and then inoculated into the culture medium. After inoculation, it is placed inside the tank 1 and the culture medium is evenly mixed by stirring rod 7. S3: When there is a lot of foam, the drive motor 5 drives the rotating rod 6 and the defoaming plate 10 to rotate. When the defoaming plate 10 rotates, it will break the foam through the protruding teeth below, and in conjunction with the addition structure and the delivery structure, the defoamer will be evenly spread on the foam to make the foam disappear. S4: After adding air into the tank 1, the drive motor 5 drives the rotating rod 6 and the stirring rod 7 to rotate so that the air is evenly distributed, and the defoaming component eliminates the air bubbles attached to the stirring rod 7.
[0028] In this embodiment, to address the issue that a single defoaming plate 10 is insufficient to handle excessive foam, when fermentation is required, the material can be added to the tank 1. The drive motor 5 rotates the rotating rod 6 and stirring rod 7, causing the stirring rod 7 to agitate the material and promote fermentation. An arc-shaped plate 46 is installed inside the tank 1, which, when the stirring rod 7 rotates, works in conjunction with the arc-shaped plate 46 to ensure uniform mixing of the material. When excessive foam appears above the material, a float 11 is provided at the end of the defoaming plate 10. This allows the defoaming plate 10 to float above the material, facilitating contact between the scraper teeth below the float 11 and the floating foam. Furthermore, when the water level changes, the height of the float 11, defoaming plate 10, and collar 9 can be adjusted. The degree will also change accordingly. The collar 9 can be kept connected to the rotating rod 6 by the cooperation of the protrusion 13 and the groove 12. When the rotating rod 6 rotates, since the collar 9 is stuck inside the groove 12 by the protrusion 13, the collar 9 can rotate with the rotating rod 6 through the engagement of the protrusion 13. When the collar 9 rotates, it will drive the four sets of defoaming plates 10 to rotate together. The multiple sets of scraping teeth set at the bottom of the defoaming plate 10 can improve the shearing force on the foam when the defoaming plate 10 rotates, thereby facilitating the elimination of larger foam. When it is necessary to add defoaming agent to the storage tank 19 of the defoaming plate 10, the defoaming agent can be injected into the sterile filter 15 so that the defoaming agent is filtered to achieve a sterile state. The filtered defoaming agent will flow into the flow channel 16 and pass through the flow channel 1. 6 flows into the connecting pipe 17 and the telescopic pipe 18. Through the telescopic pipe 18, the connection between the connecting pipe 17 and the storage tank 19 inside the defoaming plate 10 can be maintained even when the height of the defoaming plate 10 changes. After the defoamer enters the storage tank 19 through the telescopic pipe 18, since the connecting ring 20 is connected to the four sets of defoaming plates 10, the defoamer in the storage tank 19 of one set of defoaming plates 10 will flow to the other three sets of storage tanks 19 through the connecting groove 21 in the connecting ring 20. This allows the defoamer to be evenly distributed in the storage tanks 19 of the four sets of defoaming plates 10. When the defoaming plate 10 remains stationary, the pull plate 28 can be pushed to one side by the first spring 27. When the pull plate 28 is pushed, it will drive the pull rod 24 and the baffle 22 on one side to move to one side together. The thrust allows the baffle 22 and sealing gasket 23 to adhere tightly to the port of the guide groove 25, preventing the defoamer from flowing out. When the defoamer plate 10 rotates, it drives the first spring 27, pull plate 28, pull rod 24, and baffle 22 to rotate together. Due to inertia, the pull plate 28, pull rod 24, and baffle 22 generate a force opposite to that of the first spring 27, thus offsetting part of the spring's thrust and causing the pull plate 28, pull rod 24, and baffle 22 to move slightly to one side. When the baffle 22 moves away from the port of the guide groove 25, the defoamer in the storage tank 19 flows outward through the guide groove 25. Since multiple sets of guide grooves 25 are arranged in parallel, the range of defoamer dispensing can be expanded. After the defoamer comes into contact with the foam, it helps to eliminate the foam.The continuous rotation of the defoaming plate 10 agitates the foam and defoamer with its scraper teeth at the bottom, ensuring better contact between the defoamer and the foam. The rotation of the defoaming plate 10 also drives the float 11, mounting plate 29, and scraper 30 to rotate together. Since the scraper 30 is attached to the inner wall of the tank 1 and is angled, its rotation scrapes off the foam adhering to the inner wall of the tank 1. The angled shape of the scraper 30 guides the foam downwards, allowing it to continue floating on the material and preventing foam accumulation on the inner wall of the tank 1. After air is introduced into the tank 1, some air bubbles will adhere to the connection between the stirring rod 7 and the rotating rod 6. When the stirring rod 7 rotates, centrifugal force is generated, which causes the push plate 35 in the groove 33 of the stirring rod 7 to exert an outward force, thereby enabling the push plate 35 to squeeze the second spring 34 to one side. When the push plate 35 moves to one side, it also drives the connecting block 36, connecting rod 39, side rod 40 and two sets of push plates 32 to move to one side together. When the push plates 32 move, they can push the passing air bubbles to the surroundings, thereby preventing air bubbles from adhering to the outer wall of the stirring rod 7. When the stirring rod 7 stops rotating, the centrifugal force on the push plate 35, connecting block 36, connecting rod 39, side rod 40 and two sets of push plates 32 will disappear. At this time, the second spring 34 will be released. The restoring force of the spring 34 will push the push plate 35 to one side. When the push plate 35 moves, it will drive the connecting block 36, connecting rod 39, side rod 40 and two sets of push plates 32 to reset together. When the two sets of push plates 32 reset, the conical teeth on one side of the push plate 32 can puncture the air bubbles at the connection between the stirring rod 7 and the rotating rod 6, which can prevent air bubbles from adhering to the connection between the stirring rod 7 and the rotating rod 6. When the connecting block 36 moves, the remaining position of the square groove can be sealed by the two sets of sealing plates 37. By setting the sliding grooves 38 on both sides of the square groove, the two sets of sealing plates 37 can be stored inside the sliding grooves 38, so that when the connecting block 36 moves, the sealing plates 37 can keep the square groove sealed as the connecting block 36 moves. When the temperature inside the detection head tank 1 is high, the solenoid valve can be opened, and the cooling water can flow into the cooling shell 41 through the inlet 42 by opening the solenoid valve. The water is cooled by the heat conduction of the liquid, and the water that has absorbed heat is discharged through the outlet 43.
[0029] The above specific embodiments are merely several optional embodiments of the present invention. Based on the technical solutions of the present invention and the relevant teachings of the above embodiments, those skilled in the art can make various alternative improvements and combinations to the above specific embodiments.
Claims
1. A fermenter for biotechnology, comprising a tank body (1), a cover plate (3) fixedly connected to the top of the tank body (1), a drive motor (5) fixedly connected to the center of the top of the cover plate (3), a rotating rod (6) fixedly connected to the output end of the drive motor (5), the bottom of the rotating rod (6) extending into the tank body (1), and a stirring rod (7) fixedly connected to the outer wall of the rotating rod (6), characterized in that: The outer wall of the rotating rod (6) is provided with a defoaming component for eliminating foam, the defoaming component including a breaking structure, an adding structure and a feeding structure; The breaking structure is used to divide the foam and cause the foam to break. The breaking structure includes a defoaming plate (10) which is mounted on the rotating rod (6). The addition structure is used to add defoamer into the defoaming plate (10); The delivery structure is used to uniformly deliver the defoamer inside the defoaming plate (10), and the delivery structure is set on the defoaming plate (10); It also includes a defoaming assembly for eliminating air bubbles attached to the stirring rod (7).
2. The fermenter for biotechnology according to claim 1, characterized in that, The crushing structure also includes a collar (9), which is sleeved on the outer wall of the rotating rod (6). The defoaming plate (10) is fixed to one side of the collar (9). The bottom of the defoaming plate (10) is provided with multiple sets of scraping teeth. The outer wall of the rotating rod (6) is provided with a groove (12). The inner wall of the collar (9) is fixed with a protrusion (13). The protrusion (13) is slidably connected inside the groove (12). The outer wall of the rotating rod (6) is equipped with two sets of circular plates (14). The two sets of circular plates (14) are respectively installed at the top and bottom of the groove (12). The end of the defoaming plate (10) away from the collar (9) is fixed with a float (11). The defoaming plate (10) and the float (11) are each provided with four sets, which are distributed in a circle on the outer wall of the collar (9).
3. A fermenter for biotechnology according to claim 2, characterized in that, The added structure includes a sterile filter (15), which is fixed to the outer wall of the rotating rod (6). The sterile filter (15) is located at the top of the cover plate (3). The inner wall of the rotating rod (6) is provided with a flow groove (16). The outer wall of the rotating rod (6) is also fixed with a connecting pipe (17). The connecting pipe (17) is located at the bottom of the cover plate (3). The connecting pipe (17) is connected to the sterile filter (15) through the flow groove (16). The bottom of the connecting pipe (17) is fixed with a telescopic pipe (18). The bottom of the telescopic pipe (18) is fixed to the top of the defoaming plate (10). The interior of the defoaming plate (10) is provided with a storage groove (19). A connecting ring (20) is fixed between the four sets of defoaming plates (10). The interior of the connecting ring (20) is provided with a connecting groove (21). The four sets of storage grooves (19) are connected through the connecting groove (21).
4. A fermenter for biotechnology according to claim 3, characterized in that, The delivery structure includes a baffle (22), which is disposed on one side of the defoaming plate (10). A guide groove (25) is provided on one side of the storage tank (19). The baffle (22) is disposed at the port of a set of guide grooves (25). A sealing gasket (23) is fixedly connected to one end of the baffle (22) near the guide groove (25). An installation groove (26) is provided inside the defoaming plate (10). A first spring (27) is fixedly connected to the inner wall of the installation groove (26). A pull plate (28) is fixedly connected to one end of the first spring (27). A pull rod (24) is fixedly connected to one side of the pull plate (28). One end of the pull rod (24) extends out of the installation groove (26) and is fixedly connected to one side of the baffle (22).
5. A fermenter for biotechnology according to claim 4, characterized in that, A mounting plate (29) is fixed to one side of the float (11), and a scraper (30) is fixed to one side of the mounting plate (29). The scraper (30) is inclined and one side of the scraper (30) is attached to the inner wall of the tank (1). A reinforcing block (31) is fixed to one side of the scraper (30). The reinforcing block (31) is triangular in shape. Both the scraper (30) and the reinforcing block (31) are made of rubber.
6. A fermenter for biotechnology according to claim 5, characterized in that, The defoaming assembly includes a pusher plate (32), which is disposed at the top of the stirring rod (7). The stirring rod (7) has a hollow groove (33) inside. A second spring (34) is fixed to one side of the hollow groove (33). A push plate (35) is fixed to one end of the second spring (34). A connecting block (36) is fixed to the top of the push plate (35). A square groove is disposed at the top of the stirring rod (7). The connecting block (36) is slidably connected inside the square groove. A connecting rod (39) is fixed to the top of the connecting block (36). The connecting rod (39) is fixed to one end of the pusher plate (32). There are two sets of pusher plates (32), which are symmetrically distributed at the top and bottom of the stirring rod (7). The two sets of pusher plates (32) are connected by a side rod (40).
7. A fermenter for biotechnology according to claim 6, characterized in that, The square groove has sliding grooves (38) on both sides, and the connecting block (36) has sealing plates (37) fixedly connected to both sides. The sealing plates (37) are slidably connected to the inner wall of the sliding groove (38).
8. A fermenter for biotechnology according to claim 7, characterized in that, A cooling shell (41) is fixed to the outer wall of the tank (1). A water inlet (42) is fixed to one side of the cooling shell (41). A temperature sensor (44) is fixed to one side of the cooling shell (41). A solenoid valve is fixed to the outer wall of the water inlet (42). The temperature sensor (44) is connected to the solenoid valve via a wire. A detection probe (45) is fixed to one end of the temperature sensor (44). One end of the detection probe (45) extends into the tank (1). A water outlet (43) is fixed to the other side of the cooling shell (41).
9. A fermenter for biotechnology according to claim 8, characterized in that, The inner wall of the tank (1) is fixed with an arc plate (46), which is arranged between the upper and lower sets of stirring rods (7). There are three sets of arc plates (46), which are distributed in a circle on the inner wall of the tank (1).
10. A fermentation method for biotechnology, applied to the biotechnology fermenter described in claim 9, characterized in that, The method includes the following steps: S1: Clean the inside of the fermenter, remove the residue from the previous batch, and sterilize the inside of the fermenter with steam; S2: The culture medium is sterilized at high temperature and inoculated into the culture medium. After inoculation, it is placed inside the tank (1) and the culture medium is mixed evenly by stirring rod (7). S3: When there is a lot of foam, the drive motor (5) drives the rotating rod (6) and the defoaming plate (10) to rotate. When the defoaming plate (10) rotates, it will break the foam through the protruding teeth below, and in conjunction with the addition structure and the delivery structure, the defoamer will be evenly spread on the foam to make the foam disappear. S4: After adding air into the tank (1), the air is evenly distributed by driving the rotating rod (6) and stirring rod (7) to rotate by the drive motor (5), and the bubbles attached to the stirring rod (7) are eliminated by the defoaming component.