Biological carbon sequestration metering method for aquaculture of fish, shrimp, freshwater mussel and algae

Abstract

The invention provides a fish, shrimp, freshwater mussel and algae aquaculture organism carbon sequestration metering method which comprises the following steps: (1) collecting aquaculture organisms to obtain a sample and sample information; classifying the samples according to different species to obtain classification information and classified samples; (2) respectively drying the classified samples to constant weight to obtain weight information and dried samples; (3) respectively grinding the dried samples into powder to obtain ground samples; (4) determining the carbon content in the ground sample by using an elemental analyzer to obtain a carbon content coefficient; and (5) estimating the carbon reserve in combination with the sample information, the classification information, the weight information and the carbon content coefficient.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of aquaculture biological carbon fixation, and particularly relates to a fish, shrimp, mussel and algae aquaculture biological carbon fixation measurement method. BACKGROUND

[0002] At present, the global climate change situation is becoming more and more severe, and carbon neutralization has become the core issue of widespread concern in the whole society. In the field of agriculture, especially in the aquaculture industry, as a potential carbon sink, it has a considerable carbon fixation capacity. However, most of the past carbon sink researches focus on forest, wetland and grassland ecosystems, and there is a lack of systematic and standardized carbon fixation calculation method for the carbon sink estimation of aquaculture industry, especially the comprehensive ecological aquaculture system.

[0003] The "fish, shrimp, mussel and algae" aquaculture mode is a typical ecological aquaculture example, which covers multiple trophic levels: fish as top consumers, shrimps as bottom scavengers, mussels for water purification to achieve carbon fixation, and water plants and algae for photosynthesis to convert CO2 into organic carbon. This system integrates energy recycling, material recycling and ecological regulation functions, which is undoubtedly the key development direction of future green and low-carbon aquaculture. However, at present, there is still a lack of operable and scientific precise biological carbon fixation estimation method for it, which to a large extent restricts its deep application in the field of carbon sink trading and environmental assessment.

[0004] At present, the method for measuring the carbon fixation of marine organisms has been initially developed. For example, the invention patent with the patent number CN201711219935.9 "a method for measuring the carbon fixation of marine organisms" innovatively classifies marine organisms according to species, and disassembles different organs to calculate the existing carbon storage, the removed carbon storage, the deposited carbon storage and the total carbon storage of marine organisms through the existing biomass. However, this method focuses on the overall carbon fixation measurement of marine organisms, and has not carried out detailed calculation for the unique comprehensive aquaculture mode of "fish, shrimp, mussel and algae". In the aspect of carbon sink accounting of shellfish culture, the invention patent with the patent number CN201710219482.3 "a method for measuring the carbon fixation of filter-feeding shellfish" specifically formulates the carbon sink accounting process and method of shellfish culture, which lays a solid foundation for the carbon sink accounting of shellfish culture, but it is not capable of calculating the carbon fixation of "fish, shrimp, mussel and algae" in the multi-biological co-culture situation. In addition, for the calculation of algal carbon fixation, some studies follow the calculation idea of Zuo An et al., that is, the algal carbon fixation is calculated according to the yield and carbon content of algae. However, in the comprehensive aquaculture system, there are complex interactions between algae and fish, shrimp, mussel and other organisms, and this calculation method does not fully consider the potential influence of these ecological relationships on carbon fixation.

[0005] Current technologies have limitations in calculating biological carbon sequestration in integrated aquaculture models involving fish, shrimp, clams, grass, and algae. To accurately assess the amount of biological carbon sequestration in this model, a more comprehensive and refined calculation method is urgently needed. Summary of the Invention

[0006] The purpose of this invention is to provide a method for measuring biological carbon sequestration in aquaculture of fish, shrimp, clams, grasses, and algae, covering the carbon fixation process of species at different trophic levels, and realizing a comprehensive and dynamic assessment of the carbon sink function within the system.

[0007] This invention provides a method for measuring biological carbon sequestration in aquaculture of fish, shrimp, clams, grasses, and algae, the method comprising:

[0008] (1) Collect aquaculture organisms to obtain samples and sample information; classify the samples according to different species to obtain classification information and classified samples;

[0009] (2) Dry the classified samples to constant weight to obtain weight information and dried samples;

[0010] (3) Grind the dried samples into powder to obtain the ground samples;

[0011] (4) Use an elemental analyzer to determine the carbon content in the ground sample and obtain the carbon content coefficient;

[0012] (5) Estimate carbon reserves by combining sample information, classification information, weight information and carbon content coefficient.

[0013] Further, step (5) specifically includes: using the determined carbon content coefficient, combined with the data on the aquaculture area, aquaculture density, and biomass of each species in the aquaculture area, as well as sample information, classification information, and weight information, the carbon sink flux model is used to estimate the carbon storage of the entire aquaculture area.

[0014] Furthermore, step (5) also includes:

[0015] The fish, shrimp, and clam portions of the sample were based on the individual biomass accumulation method, as shown in Formula I:

[0016]

[0017] In Formula I, Ni is the number of individuals in the i-th class of animals, and W i For average fresh weight growth, f c The carbon content coefficient;

[0018] The aquatic plants and algae in the sample were estimated using the net primary productivity method, as shown in Formula II:

[0019] C = NPP·fc • A·T formula II;

[0020] In formula II, NPP is net primary productivity, f c is carbon content coefficient, A is area, and T is continuous days;

[0021] Further, step (5) further comprises:

[0022] The total carbon fixation amount is calculated according to formula III:

[0023] C 总 =C 鱼 +C 虾 +C 蚌 +C 草 +C 藻 Formula III;

[0024] Considering the factors of respiration, death decomposition and carbon transfer of excrement, the actual total carbon fixation amount is corrected according to formula IV:

[0025] C 终 =C 总 (1-L) formula IV;

[0026] In formula IV, L is carbon loss rate, and L is 10%-30%.

[0027] Further, step (1) specifically comprises: collecting aquatic farming organisms in an aquatic farming area and corresponding statistics of the quantity to obtain samples and sample information; the collected samples are placed in an incubator; the incubator has heat insulation performance and a temperature and humidity adjusting system, and is used to ensure that the quality of the samples is not affected during transportation; the samples are classified according to different species and placed in containers with labels, and the labels record the information of the species name, collection location and collection time in detail, to obtain classification information and classified samples.

[0028] Further, step (2) specifically comprises: placing the classified samples on a stainless steel drying tray, and placing the drying tray into a vacuum drying box to dry to constant weight, to obtain weight information and dried samples.

[0029] Further, step (3) specifically comprises: grinding the dried samples into powder by using a grinding device, and then screening the ground samples by using a screen, to obtain ground samples.

[0030] Further, step (4) specifically comprises: measuring the carbon content in the ground samples by using an elemental analyzer, and calculating the carbon content coefficient of aquatic farming organisms of different species according to the measurement results.

[0031] Advantages of the present application:

[0032] (1) The fish, shrimp, mussel, grass and algae aquaculture biological carbon fixation measurement method of the present application has the beneficial effects of high accuracy, comprehensiveness, simple operation and great application value. By measuring the biomass and carbon content coefficient of different organisms such as fish, shrimp, mussel, grass and algae, and combining with the corresponding carbon fixation formula calculation, the biological carbon fixation amount of the aquaculture system can be more accurately reflected. Considering the role of various organisms in the carbon fixation process, the main biological components are covered, making the calculation results representative and complete. The determination method and calculation formula used are simple and easy to operate, without the need for complex equipment and tedious calculation.

[0033] (2) The method provides a scientific basis for aquaculture practitioners to understand carbon sink contribution, is conducive to formulating breeding planning and management measures, improving ecological and economic benefits, and at the same time provides data and technical support for government departments to formulate carbon emission reduction policies and assess carbon sink projects, which is of great significance to respond to climate change and achieve carbon peak and carbon neutralization targets. DETAILED DESCRIPTION

[0034] The present application will be described in detail below in conjunction with specific embodiments.

[0035] The two ponds used in the present application are rectangular and cubic structures. The water area of the rectangular pond is 2000m 2 , the average water depth is 2.5m, the average water replenishment is 100m 3 / d, and the evaporation and discharge amount is 100m 3 / d. The water area of the cubic pond is 3600m 2 , the average water depth is 1.8m, the average water replenishment is 150m 3 / d, and the evaporation and discharge amount is 150m 3 / d.

[0036] The fish, shrimp, mussel, grass and algae aquaculture biological carbon fixation measurement method of the present application comprises the following steps:

[0037] A large fishing net is used to collect water aquaculture biological samples in a specific aquaculture area and the number is counted accordingly. The collected samples are placed in specially designed incubators which have good heat insulation performance and stable temperature and humidity adjustment system to ensure that the quality of the samples is not affected during transportation.

[0038] The water aquaculture organisms are classified according to different species and placed in containers with labels. The labels record detailed information such as species name, collection site and collection time.

[0039] The samples are dried and placed on stainless steel drying trays in a vacuum drying oven until they reach a constant weight.

[0040] The sample dried to constant weight is ground into powder using a grinding device, and after grinding, the powder is screened using a screen to ensure that the particle size of the powder is uniform and within the required particle size range for the experiment.

[0041] The carbon content in the sample is measured using an elemental analyzer, and based on the results of multiple measurements, the carbon content coefficient of the species of aquatic organisms is calculated. This coefficient can reflect the absorption and accumulation ability of the species to carbon elements during growth.

[0042] By determining the carbon content coefficient, combined with the data of the aquaculture area, the breeding density, and the biomass of each species in the aquaculture area, the carbon storage of the entire aquaculture area is estimated using a carbon sink flux model.

[0043] Example 1

[0044] The water area of the square pond is 3600m 2 , the average water depth is 1.8m, the average water replenishment is 150m 3 / d, and the evaporation and discharge is 150m 3 / d. In this ecosystem, the pH, COD, ammonia nitrogen, TN, and TP of the water body are in the range of 6-8, 7-26mg / L, 0.2-1.1mg / L, 0.6-1.4mg / L, and 0.1-0.3mg / L, respectively, meeting the IV standard of the Environmental Quality Standards for Surface Water (GB 3838-2002).

[0045] The pond needs to be cleaned before use, and the water depth needs to be controlled during use. A certain amount of grass carp, small river clams, triangle sail bivalves, elodea and chlorella are put into the pond, and the carbon fixation effect after 3 months is as follows.

[0046]

[0047] Biological carbon estimation:

[0048] Fish carbon fixation: C 鱼 = 3000 x 75 x 0.12 = 27000g = 27kg

[0049] Shrimp carbon fixation: C 虾 = 7000 x 4 x 0.19 = 5320g = 5.32kg

[0050] Mussel carbon fixation: C 蚌 = 1500 x 10 x 0.11 = 1650g = 1.65kg

[0051] Grass carbon fixation: C 草= 3600 x 7 x 0.37 x 90 = 839160 g = 839.16 kg

[0052] Algal carbon fixation: C 藻 = 6480 x 1 x 0.42 x 90 = 244944 g = 244.944 kg

[0053] Total carbon and loss adjustment:

[0054] Total carbon fixation (preliminary): C 总 = 27 + 5.32 + 1.65 + 839.16 + 244.94 = 1118.07 kg

[0055] Take the carbon loss rate of 15%, the final net carbon fixation is:

[0056] C 终 = 1118.07 x (1 - 0.15) = 950.3595 kg

[0057] Example 2

[0058] The square pond water area is 3600 m 2 , the average water depth is 1.8 m, the average water replenishment is 150 m 3 / d, the evaporation and discharge is 150 m 3 / d. In this ecosystem, the pH, COD, ammonia nitrogen, TN, TP of the water body are respectively in the range of 6-8, 7-26 mg / L, 0.2-1.1 mg / L, 0.6-1.4 mg / L, 0.1-0.3 mg / L, which meets the IV class standard of "Surface Water Environmental Quality Standard (GB 3838-2002)".

[0059] The pond needs to be cleaned before use, and the water depth needs to be controlled during use. A certain amount of grass carp, small river clam, triangle sail bivalve, elodea and chlorella are put into the pond, and the carbon fixation effect after 6 months of putting is as follows.

[0060]

[0061] Biological carbon estimation:

[0062] Fish carbon fixation: C 鱼 = 3000 x 130 x 0.12 = 46800 g = 46.8 kg

[0063] Shrimp carbon fixation: C 虾 = 7000 x 7 x 0.19 = 9310 g = 9.31 kg

[0064] Clam carbon fixation: C 蚌= 1500 x 18 x 0.11 = 2970 g = 2.97 kg

[0065] Grass carbon sequestration: C 草 = 3600 x 7 x 0.37 x 181 = 1687644 g = 1687.644 kg

[0066] Algae carbon sequestration: C 藻 = 6480 x 1 x 0.42 x 181 = 492609.6 g = 492.6096 kg

[0067] Total carbon and loss adjustment:

[0068] Total carbon sequestration (preliminary): C 总 = 46.8 + 9.31 + 2.97 + 1687.644 + 492.6096 = 2239.3336 kg

[0069] Take the carbon loss rate as 15%, the final net carbon sequestration is:

[0070] C 终 = 2239.3336 x (1-0.15) = 1903.43356 kg

[0071] Example 3

[0072] The square pond water area is 3600 m 2 , the average water depth is 1.8 m, the average water replenishment is 150 m 3 / d, the evaporation and discharge is 150 m 3 / d. In this ecosystem, the pH, COD, ammonia nitrogen, TN, TP of the water body are respectively in the range of 6-8, 7-26 mg / L, 0.2-1.1 mg / L, 0.6-1.4 mg / L, 0.1-0.3 mg / L, which meets the IV class standard of "Surface Water Environmental Quality Standard (GB 3838-2002)".

[0073] The pond needs to be cleaned before use in the invention, and the water depth needs to be controlled during use. A certain amount of grass carp, small river clam, triangle sail bivalve, elodea and chlorella are put into the pond, and the carbon sequestration effect after 9 months of putting is as follows.

[0074]

[0075] Biological carbon estimation:

[0076] Fish carbon sequestration: C 鱼 = 3000 x 165 x 0.12 = 59400 g = 59.4 kg

[0077] Shrimp carbon sequestration: C 虾= 7000 x 9 x 0.19 = 11970 g = 11.97 kg

[0078] Mussel carbon fixation: C 蚌 = 1500 x 23 x 0.11 = 3795 g = 3.795 kg

[0079] Grass carbon fixation: C 草 = 3600 x 7 x 0.37 x 273 = 2545452 g = 2545.452 kg

[0080] Algae carbon fixation: C 藻 = 6480 x 1 x 0.42 x 273 = 742996.8 g = 742.9968 kg

[0081] Total carbon and loss adjustment:

[0082] Total carbon fixation (preliminary): C 总 = 59.4 + 11.97 + 3.795 + 2545.452 + 742.9968 = 3363.6138 kg

[0083] Take the carbon loss rate of 15%, the final net carbon fixation is:

[0084] C 终 = 3363.6138 x (1 - 0.15) = 2859.07173 kg

[0085] Example 4

[0086] The square pond water area is 3600 m 2 , the average water depth is 1.8 m, the average water replenishment is 150 m 3 / d, the evaporation and discharge is 150 m 3 / d. In this ecosystem, the pH, COD, ammonia nitrogen, TN, TP of the water body are in the range of 6-8, 7-26 mg / L, 0.2-1.1 mg / L, 0.6-1.4 mg / L, 0.1-0.3 mg / L, respectively, which meets the IV standard of "Surface Water Environmental Quality Standard (GB 3838-2002)".

[0087] The pond needs to be cleaned before use in the invention, and the water depth needs to be controlled during use. A certain amount of grass carp, small river mussel, triangle mussel, elodea and chlorella are put into the pond, and the carbon fixation effect after 12 months of putting is as follows.

[0088]

[0089] Biological carbon estimation:

[0090] Fish carbon fixation: C 鱼= 3000 x 185 x 0.12 = 66600 g = 66.6 kg

[0091] Carbon fixation of shrimp: C 虾 = 7000 x 10 x 0.19 = 13300 g = 13.3 kg

[0092] Carbon fixation of mussel: C 蚌 = 1500 x 26 x 0.11 = 2640 g = 2.64 kg

[0093] Carbon fixation of grass: C 草 = 3600 x 7 x 0.37 x 365 = 3403260 g = 3403.26 kg

[0094] Carbon fixation of algae: C 藻 = 6480 x 1 x 0.42 x 365 = 993384 g = 993.384 kg

[0095] Total carbon and loss adjustment:

[0096] Total carbon fixation (preliminary): C 总 = 66.6 + 13.3 + 2.64 + 3403.26 + 993.384 = 4479.184 kg

[0097] Taking the carbon loss rate as 15%, the final net carbon fixation amount is:

[0098] C 终 = 4479.184 x (1 - 0.15) = 3807.3064 kg

[0099] Example 5

[0100] The cuboid pond has a water body area of 2000 m 2 , an average water depth of 2.5 m, an average water replenishment of 100 m 3 / d, and an evaporation and discharge amount of 100 m 3 / d. In this ecosystem, the pH, COD, ammonia nitrogen, TN, and TP of the water body are in the ranges of 6-8, 10-30 mg / L, 0.3-1.2 mg / L, 0.5-1.5 mg / L, and 0.1-0.2 mg / L, respectively, meeting the IV class standard of the Environmental Quality Standards for Surface Water (GB 3838-2002).

[0101] The pond needs to be cleaned before use, and the water depth needs to be controlled during use. A certain amount of grass carp, small river mussel, triangle mussel, elodea and chlorella are put into the pond, and the carbon fixation effect after 3 months is as follows.

[0102]

[0103] Estimation of the amount of biological carbon:

[0104] Carbon fixation of fish: C 鱼 = 2000 x 50 x 0.11 = 11000 g = 11 kg

[0105] Carbon fixation of shrimp: C 虾 = 5000 x 2 x 0.17 = 1700 g = 1.7 kg

[0106] Carbon fixation of mussel: C 蚌 = 1000 x 5 x 0.12 = 600 g = 0.6 kg

[0107] Carbon fixation of grass: C 草 = 2000 x 8 x 0.35 x 90 = 504000 g = 504 kg

[0108] Carbon fixation of algae: C 藻 = 5000 x 1 x 0.45 x 90 = 202500 g = 202.5 kg

[0109] Total carbon amount and loss adjustment:

[0110] Total carbon fixation amount (preliminary): C 总 = 11 + 1.7 + 0.6 + 504 + 202.5 = 719.8 kg

[0111] Taking the carbon loss rate as 15%, the final net carbon fixation amount is:

[0112] C 终 = 719.8 x (1 - 0.15) = 611.83 kg

[0113] Example 6

[0114] The cuboid pond has a water body area of 2000 m 2 , an average water depth of 2.5 m, an average water replenishment of 100 m 3 / d, and an evaporation and discharge amount of 100 m 3 / d. In this ecological system, the pH, COD, ammonia nitrogen, TN and TP of the water body are in the ranges of 6-8, 10-30 mg / L, 0.3-1.2 mg / L, 0.5-1.5 mg / L and 0.1-0.2 mg / L, respectively, which meet the IV class standard of the Environmental Quality Standards for Surface Water (GB 3838-2002).

[0115] The pond needs to be cleaned before use, and the water depth needs to be controlled during use. A certain amount of grass carp, small river mussel, triangle mussel, elodea and chlorella are put into the pond, and the carbon fixation effect after 6 months is as follows.

[0116]

[0117] Estimation of the amount of biological carbon:

[0118] Carbon fixation of fish: C 鱼 = 2000 x 100 x 0.11 = 22000 g = 22 kg

[0119] Carbon fixation of shrimp: C 虾 = 5000 x 4 x 0.17 = 3400 g = 3.4 kg

[0120] Carbon fixation of mussel: C 蚌 = 1000 x 8 x 0.12 = 960 g = 0.96 kg

[0121] Carbon fixation of grass: C 草 = 2000 x 8 x 0.35 x 181 = 1013600 g = 1013.6 kg

[0122] Carbon fixation of algae: C 藻 = 5000 x 1 x 0.45 x 181 = 407250 g = 407.25 kg

[0123] Total carbon amount and loss adjustment:

[0124] Total carbon fixation amount (preliminary): C 总 = 22 + 3.4 + 0.96 + 1013.6 + 407.25 = 1447.21 kg

[0125] Taking the carbon loss rate as 15%, the final net carbon fixation amount is:

[0126] C 终 = 1447.21 x (1 - 0.15) = 1230.1285 kg

[0127] Example 7

[0128] The cuboid pond water area is 2000 m 2 , the average water depth is 2.5 m, the average water replenishment is 100 m 3 / d, the evaporation and discharge amount is 100 m 3 / d. In this ecosystem, the pH, COD, ammonia nitrogen, TN, TP of the water body are respectively in the range of 6-8, 10-30 mg / L, 0.3-1.2 mg / L, 0.5-1.5 mg / L, 0.1-0.2 mg / L, which meets the IV class standard of “Surface Water Environmental Quality Standard (GB 3838-2002)”.

[0129] The pond in the present application needs to be cleaned before use, and the water depth needs to be controlled during use. A certain amount of grass carp, small river bivalve, triangle bivalve, elodea and chlorella are put into the pond. After 9 months of putting, the carbon fixation effect is as follows.

[0130]

[0131] Biological carbon estimation:

[0132] Fish carbon fixation: C 鱼 = 2000 x 150 x 0.11 = 33000 g = 33 kg

[0133] Shrimp carbon fixation: C 虾 = 5000 x 6 x 0.17 = 5100 g = 5.1 kg

[0134] Carbon fixation of bivalve: C 蚌 = 1000 x 10 x 0.12 = 1200 g = 1.2 kg

[0135] Carbon fixation of grass: C 草 = 2000 x 8 x 0.35 x 273 = 1528800 g = 1528.8 kg

[0136] Algal carbon fixation: C 藻 = 5000 x 1 x 0.45 x 273 = 614250 g = 614.25 kg

[0137] Total carbon and loss adjustment:

[0138] Total carbon fixation (preliminary): C 总 = 33 + 5.1 + 1.2 + 1528.8 + 614.25 = 2182.35 kg

[0139] Take the carbon loss rate as 15%, the final net carbon fixation amount is:

[0140] C 终 = 2182.35 x (1-0.15) = 1854.9975 kg

[0141] Example 8

[0142] The cuboid pond has a water area of 2000 m 2 , the average water depth is 2.5 m, the average water replenishment is 100 m 3 / d, and the evaporation and discharge amount is 100 m 3In this ecosystem, the pH, COD, ammonia nitrogen, TN, TP of the water body are in the range of 6-8, 10-30 mg / L, 0.3-1.2 mg / L, 0.5-1.5 mg / L, 0.1-0.2 mg / L, respectively, meeting the IV standard of the Environmental Quality Standard for Surface Water (GB 3838-2002).

[0143] The pond in the present application needs to be cleaned before use, and the water depth needs to be controlled during use. A certain amount of grass carp, small river bivalve, triangle bivalve, elodea and chlorella are put into the pond, and the carbon fixation effect after 12 months of putting is as follows.

[0144]

[0145] Biological carbon estimation:

[0146] Fish carbon fixation: C 鱼 = 2000 x 200 x 0.11 = 44000 g = 44 kg

[0147] Shrimp carbon fixation: C 虾 = 5000 x 8 x 0.17 = 6800 g = 6.8 kg

[0148] Carbon fixation of bivalve: C 蚌 = 1000 x 12 x 0.12 = 1440 g = 1.44 kg

[0149] Grass carbon fixation: C 草 = 2000 x 8 x 0.35 x 365 = 2044000 g = 2044 kg

[0150] Algae carbon fixation: C 藻 = 5000 x 1 x 0.45 x 365 = 821250 g = 821.25 kg

[0151] Total carbon and loss adjustment:

[0152] Total carbon fixation (preliminary): C 总 = 44 + 6.8 + 1.44 + 2044 + 821.25 = 2917.49 kg

[0153] Take the carbon loss rate as 15%, the final net carbon fixation amount is:

[0154] C 终 = 2917.49 x (1-0.15) = 2479.8665 kg

[0155] Those skilled in the art can understand that the above-mentioned embodiments are specific examples for realizing the present application, and in practical applications, various changes can be made in form and details without departing from the spirit and scope of the present application. Any person skilled in the art can make respective changes and modifications without departing from the spirit and scope of the present application, and therefore the protection scope of the present application should be subject to the scope defined by the claims.

Claims

1. A method for measuring biological carbon sequestration in aquaculture of fish, shrimp, clams, grass, and algae, characterized in that, The method includes: (1) Collect aquaculture organisms to obtain samples and sample information; classify the samples according to different species to obtain classification information and classified samples; (2) Dry the classified samples to constant weight to obtain weight information and dried samples; (3) Grind the dried samples into powder to obtain the ground samples; (4) Use an elemental analyzer to determine the carbon content in the ground sample and obtain the carbon content coefficient; (5) Estimate carbon reserves by combining sample information, classification information, weight information and carbon content coefficient.

2. The method for measuring biological carbon sequestration in aquaculture of fish, shrimp, clams, grasses, and algae according to claim 1, characterized in that, Step (5) specifically includes: using the determined carbon content coefficient, combined with the data on the aquaculture area, aquaculture density, and biomass of each species in the aquaculture area, as well as sample information, classification information, and weight information, the carbon sink flux model is used to estimate the carbon storage of the entire aquaculture area.

3. The method for measuring biological carbon sequestration in aquaculture of fish, shrimp, clams, grasses, and algae according to claim 2, characterized in that, Step (5) also includes: The fish, shrimp, and clam portions of the sample were based on the individual biomass accumulation method, as shown in Formula I: In Formula I, Ni is the number of individuals in the i-th class of animals, and W i For average fresh weight growth, f c The carbon content coefficient; The aquatic plants and algae in the sample were estimated using the net primary productivity method, as shown in Formula II: C = NPP·f c Formula II of A·T; In Formula II, NPP represents net primary productivity, f c Here, A is the carbon content coefficient, A is the area, and T is the duration in days.

4. The method for measuring biological carbon sequestration in aquaculture of fish, shrimp, clams, grasses, and algae according to claim 3, characterized in that, Step (5) also includes: Total carbon sequestration is calculated as shown in Formula III: C 总 =C 鱼 +C 虾 +C 蚌 +C 草 +C 藻 Formula III; Taking into account respiration, decomposition after death, and carbon transfer from excrement, the actual total carbon sequestration is corrected to Formula IV: C 终 =C 总 (1-L) Formula IV; In Formula IV, L represents the carbon loss rate, which ranges from 10% to 30%.

5. The method for measuring biological carbon sequestration in aquaculture of fish, shrimp, clams, grasses, and algae according to claim 1, characterized in that, Step (1) specifically includes: collecting aquatic organisms in the aquaculture area and making corresponding statistics on their quantity to obtain samples and sample information; The collected samples are placed in an insulated box; the insulated box has heat insulation properties and a temperature and humidity control system to ensure that the quality of the samples is not affected during transportation. The samples were classified into different species and placed in labeled containers. The labels recorded the species name, collection location and collection time in detail, thus obtaining the classification information and the classified samples.

6. The method for measuring biological carbon sequestration in aquaculture of fish, shrimp, clams, grasses, and algae according to claim 1, characterized in that, Step (2) specifically includes: placing the classified samples on a stainless steel drying tray, putting them into a vacuum drying oven, drying them to a constant weight, and obtaining weight information and the dried samples.

7. The method for measuring biological carbon sequestration in aquaculture of fish, shrimp, clams, grasses, and algae according to claim 1, characterized in that, Step (3) specifically includes: using a grinding device to grind the dried sample into powder, and after grinding, using a sieve to screen it to obtain the ground sample.

8. The method for measuring biological carbon sequestration in aquaculture of fish, shrimp, clams, grasses, and algae according to claim 1, characterized in that, Step (4) specifically includes: using an elemental analyzer to measure the carbon content in the ground sample, and calculating the carbon content coefficient of different species of aquatic organisms based on the results of multiple measurements.

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