High-speed low-cross-contamination full-automatic biochemical detection device and method

Abstract

The invention relates to a high-speed low-cross-contamination full-automatic biochemical detection device which mainly structurally comprises one or more reagent loading devices and a reaction disc, wherein more than one circle of detection cup belts which are uniformly distributed in a concentric circle are loaded on the periphery of the reaction disc; one or more sample loading devices; the stirring device is used for stirring the sample reagent liquid in the detection cup; the optical detection unit is used for detecting all the detection cups loaded on the reaction disc; one or more than one multi-stage detection cup cleaning device; the device is provided with more than one reagent arm and more than one sample arm, and each reagent arm and each sample arm are respectively provided with a liquid suction needle for quantitatively sucking and distributing reagents and samples; the device is also provided with special needle cleaning pools for reagent needles and sample needles, so that the inside and the outside of the sample needles and the reagent needles are cleaned, and the carrying pollution of the needles is reduced. For the contact type stirring device, a corresponding cleaning device is also arranged to clean the contact stirring part of the stirring device.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of medical examination and detection, in particular to a high-speed low-cross-contamination full-automatic biochemical detection device and method. BACKGROUND

[0002] This part provides only background information related to the present disclosure, which is not necessarily prior art.

[0003] A high-speed low-cross-contamination full-automatic biochemical detection device used in medical examination is mainly used for detecting serum, plasma, cerebrospinal fluid, urine and other liquid samples, and obtaining important diagnostic indicators of the cause and development state of the patient's disease. With the progress of medical technology, the improvement of diagnosis level and requirements, the number of biochemical detection items is gradually increasing. In large medical institutions or specialized examination service centers, the number of samples for detection is often very large, and the number of daily detections often exceeds 5000 tests or more. The samples often need to be detected within 4-6 hours in order to provide diagnostic information for medical treatment in time, otherwise it may delay clinical diagnosis and timely treatment. In addition, various components in the samples for biochemical detection may change due to various reasons such as cell metabolism, enzyme metabolism consumption, sample volatilization, bacterial contamination growth and other factors, which may affect the accuracy of the test results. Therefore, quickly completing the biochemical detection of the sample has important value for improving the quality of the test results, and the improvement of the working efficiency of the device can also reduce the working time intensity of the examination personnel. Therefore, improving the detection speed of the biochemical instrument has many beneficial values for improving the service quality of the medical institutions.

[0004] In addition, in the clinical biochemical detection, the device is often only provided with limited reagent one needle and reagent two needle, the limited reagent needle needs to respectively suck different reagent one or different reagent two according to the detection item, and the sucked reagent is respectively added into different detection cups, and the properties, components, colors and the like of different reagents are often different, and the biochemical instrument runs at a high speed, and more time cannot be given for cleaning, especially the high-speed biochemical instrument cannot completely clean the inside and outside of the reagent needle, although the reagent needle is cleaned before or after sucking the reagent each time, but the reagent contacted before is inevitably left, and the cross of the residual reagent and the newly sucked reagent will cause the impurity of the used detection reagent, the pollution of the distributed reagent, and more water is consumed for cleaning the needle each time. Under normal circumstances, the same medical institution has the same sample detection item in most cases, and the reagent type has a high reuse condition, so two or more samples for the same reagent detection can reduce the cross contamination by once combined sucking and distributing the reagent, reduce the reagent needle cleaning frequency and cleaning water consumption. In addition, the reagent needle must contact the reagent liquid in the reagent bottle to suck the reagent each time, and the reagent needle inevitably brings a small amount of other liquids such as water and other reagents into the reagent bottle when the reagent needle enters the reagent bottle, and the more the amount of other liquids brought into the reagent bottle, the more the purity of the reagent in the reagent bottle is polluted, and the frequency of the reagent needle entering the reagent bottle is reduced, so that the probability of the reagent needle bringing other liquids into the reagent bottle is effectively reduced, and the stability of the opened reagent is improved. The high-speed low-cross-contamination automatic biochemical detection device designed in the application adopts the mode of sucking multiple reagents by the reagent needle once, and the running speed pressure of the reagent needle is greatly reduced, so that the liquid dripping phenomenon of the reagent needle caused by the rapid movement of the reagent needle is reduced, and the accuracy of the reagent distribution and the adverse effect of the dripping reagent on the detection result quality are improved.

[0005] Therefore, the biochemical instrument designed in the application improves the detection speed of the device, reduces the reagent pollution of the original biochemical instrument design scheme, reduces the cleaning water consumption and waste water generation amount in the device running, and better improves the detection result quality of the biochemical instrument. In addition, the design also reduces the excessive reagent arm structure of the high-speed biochemical instrument, simplifies the device structure and manufacturing cost, and correspondingly reduces the device failure rate. SUMMARY

[0006] In view of the deficiencies of the prior art, the present application provides a high-speed low-cross-contamination fully-automatic biochemical detection device and method, the device sucks more than one portion of reagent by a reagent needle at one time, and adds the sucked more than one portion of reagent into more than one detection cup on the inner and outer detection cup belts of the reaction disc, so as to reduce the number of times of sucking reagent by the reagent needle and the number of times of cleaning the reagent needle, improve the detection speed of the device, avoid the liquid dripping phenomenon in the reagent needle caused by the fast movement of the reagent needle in the high-speed fully-automatic biochemical detection device, and improve the accuracy of reagent distribution and avoid the adverse effect of the dripping reagent on the quality of the detection result. The biochemical instrument of the design improves the detection speed of the device, and also reduces the reagent pollution of the original biochemical instrument design scheme and reduces the amount of cleaning water and the amount of waste water generated during the operation of the device, and better improves the quality of the detection result of the biochemical instrument. The high-speed biochemical instrument equipment reduces the structure of too many reagent arms, simplifies the structure and manufacturing cost of the device, and accordingly reduces the failure rate of the device.

[0007] A high-speed low-cross-contamination fully-automatic biochemical detection device, comprising a reaction disc, at least one sample loading device, and at least one reagent loading device; the device has more than one first reagent arm, more than one second reagent arm, and more than one sample arm; each reagent arm is respectively provided with a reagent needle for quantitatively sucking and dispensing reagent; each sample arm is respectively provided with a sample needle for quantitatively sucking and dispensing sample; the reaction disc and the reagent loading device quantitatively transfer reagent through the reagent needle loaded by the reagent arm, and the reaction disc and the sample loading device quantitatively transfer sample through the sample needle carried by the sample arm; the device is also provided with a cleaning device for cleaning each reagent needle and each sample needle; a circle of detection cup belts is arranged around the reaction disc, and the detection cup belts are arranged in concentric circles and are arranged at equal intervals in the circumferential direction; each detection cup belt is provided with more than one detection cup, and a plurality of stages of detection cup cleaning devices, optical detection units, and more than one stirring device are respectively arranged along the movement track of the detection cup belt. The reagent loading device can be a disc type.

[0008] The stirring device is a non-contact type or a contact type; when the stirring device is a contact type, each stirring arm has more than one stirring needle, and the stirring needles simultaneously perform synchronous stirring on the detection cups of different detection cup rings on the reaction disc. The device is also provided with a dedicated cleaning pool for the contact part of the liquid in the detection cup of the contact type stirring device. Or it can also be designed as a three-group integrated direct contact stirring device, and the three groups of direct stirring devices are in a state of being equally divided by an angle of 120°, and when the stirring device works, one group performs stirring, and the other two groups perform cleaning, which can fully clean the stirring needles and improve the stirring speed and efficiency.

[0009] Each stirring device in the apparatus has the function of simultaneously stirring four or more test cups, and each stirring device has the function of simultaneously stirring one or more different test cups on one or more different test cup belts.

[0010] Furthermore, each reagent loading device is provided with one or more reagent bottle positions. The reagent loading device includes a first reagent loading device and a second reagent loading device. The first reagent loading device quantitatively transfers reagent 1 to the reaction plate through a reagent needle 1 carried by a first reagent arm. The second reagent loading device quantitatively transfers reagent 2 to the reaction plate through a reagent needle 2 carried by a second reagent arm. Each of the first and second reagent arms has a reagent needle at one end. The reaction plate has two rings of detection cups. The trajectory of each reagent needle intersects both rings of detection cups on the reaction plate, or each reagent needle intersects only one ring of detection cups. The sample arm includes a first sample arm and a second sample arm. This arm, the first sample arm and the second sample arm, each have a sample needle at one end. The trajectory of the sample needle intersects both rings of the detection cups on the reaction plate; or each sample needle intersects only one ring of the detection cups on the reaction plate. The stirring device includes a first stirring device and a second stirring device, which can simultaneously stir one or more detection cups on each of the two rings of the detection cups on the reaction plate. When adding reagents or samples continuously, the reagent needle or sample needle can add reagents or samples to adjacent detection cups in the same ring of detection cups; or it can add reagents or samples to non-adjacent detection cups in the same ring of detection cups.

[0011] A high-speed, low-cross-contamination fully automated biochemical detection method, specifically comprising:

[0012] The reaction disk operates in a regular pattern: it rotates once and then pauses after adding at least one more detection cup position; during the pause, all reagent needles, sample needles and stirring devices simultaneously perform reagent dispensing, sample dispensing and mixing operations on one or more detection cups in the two-ring detection cup belt.

[0013] The reagent dispensing specifically includes that when the reaction disc of the device rotates forward, two first reagent arms of the device respectively draw two portions of reagent 1 from different reagent bottles on the first reagent loading device through respective first reagent needles; when the reaction disc is paused according to the specified operation mode, the two first reagent needles respectively add one portion of reagent to each of two detection cups in two detection cup belts; when the reaction disc rotates forward, the reagent needles of the two first reagent arms respectively draw two portions of reagent and then move to the intersection of a detection cup belt on the reaction disc; when the reaction disc stops rotating, one detection cup of the detection cup belt stops directly below one first reagent needle, and the other detection cup stops directly below the other first reagent needle; the two first reagent needles respectively add one portion of reagent to one detection cup through the connected diluter; then the two first reagent needles respectively move to the intersection of the other detection cup on the reaction disc, and the two first reagent needles respectively add the remaining one portion of reagent to one detection cup below each needle; that is, the two first reagent needles complete the addition of one portion of reagent to four detection cups at one time, and the two first reagent needles add different reagents.

[0014] Or when the reaction disc moves forward by one or more detection cup positions after the two first reagent needles complete the dispensing of the first group of reagents, the two first reagent needles do not return to the original reagent drawing position for cleaning, but draw the original reagents, and then return to the intersection of the needle track and the newly moved one or more detection cups to dispense one portion of reagent 1 again; the two reagent needles dispense 8 portions of reagent 1 in the process.

[0015] After the dispensing is completed, the two first reagent arms respectively move the first reagent needles to the cleaning pool for cleaning; after the cleaning is completed, the two first reagent arms respectively draw two portions of reagent in different reagent bottles through the respective reagent needles, in preparation for the next reagent dispensing; the two first reagent needles respectively draw different reagents, or can also draw the same reagents.

[0016] The sample dispensing is specifically that when the reaction disc rotates to the sample adding position and pauses, each sample needle of the sample arm has previously taken two samples, at this time each sample needle respectively adds one of the previously taken samples to one detection cup into which reagent 1 has been added through the connected diluter, and the other sample is added to another detection cup into which another reagent 1 is injected; or each sample needle respectively adds one of the previously taken samples to one detection cup, and then is cleaned or not cleaned and then returns to the sample tube from which the sample was taken, and then takes another sample from the same sample tube and respectively adds it to another different detection cup; that is, two sample needles complete the addition of one sample to four detection cups at one time of pausing of the reaction disc, and the two sample needles respectively add different samples, but the samples taken by the same sample needle come from the same sample tube. And the two sample needles respectively add two samples to two different detection cups, and each sample needle is added to two detection cups distributed by two different first reagent needles. After the above sample dispensing is completed, the sample needle carried by the sample arm moves to the cleaning pool for cleaning, and after cleaning is completed, the sample arm takes the sample again through the sample needle to prepare for the next sample dispensing. The sample taken by the sample needle is often less than the reagent amount during biochemical detection, and is only about 2%-10% of the reagent amount, so the time consumed by the sample needle for taking the sample and cleaning is much shorter than the time consumed by the reagent needle, and the cleaning and resampling of the sample needle does not greatly delay the overall device running time.

[0017] The stirring is specifically that when the reaction disc rotates forward and pauses, the first stirring device performs stirring on one or more detection cups containing reagent 1 and samples below the detection cup belt, and simultaneously performs stirring on one or more detection cups on each of the two detection cup belts, so as to fully mix and react the reagent 1 and the sample in each stirred detection cup.

[0018] The second reagent dispensing according to the detection needs is that when reagent 2 needs to be added according to the detection needs, each second reagent needle respectively takes two portions of reagent 2 in advance, and when the reaction disc rotates forward and pauses, each second reagent needle respectively adds one portion of reagent 2 to each of two detection cups on the two detection cup belts into which reagent 1, samples and the stirred detection cup have been added through the two second reagent needles; and the two portions of reagent 2 added by one of the second reagent needles correspond to the same detection cup as the two portions of reagent 1 added by one of the first reagent needles, and the two portions of reagent added by the other second reagent needle correspond to the same detection cup as the two portions of reagent 1 added by the other first reagent needle, that is, the two second reagent needles together complete the addition of reagent 2 to four detection cups at one time, and the two second reagent needles add different reagents.

[0019] The specific operation of the second stirring is that, when the reaction disc is rotating to pause, if the detection cup under the second stirring device contains reagent 1, reagent 2 and sample, the second stirring device simultaneously performs stirring in each one or more than one detection cup on the two detection cup belts, so that the reagent 1, reagent 2 and sample in the cup are fully mixed and reacted.

[0020] In the detection process, no reagent 2 is added for detection, and the second stirring device does not perform stirring on the detection cup.

[0021] The optical detection and cleaning includes that the optical signal detection unit synchronously performs detection on each detection cup arranged on each detection cup belt of the reaction disc, that is, each detection cup is detected once after receiving reagent 1, adding sample and stirring, adding reagent 2 and stirring, and before and after each detection cup is operated each time, and each detection cup is detected once when passing through the optical signal detection unit, until each detection cup completes detection and enters the multi-stage cleaning device, the reaction liquid in the detection cup is removed and cleaned multiple times in the multi-stage cleaning device, and the detection cup after cleaning is recycled for detection, until the required detection sample completes all detection.

[0022] A high-speed and low-cross contamination automatic biochemical detection method and device, the device includes two first reagent arms with different motion trajectories and two second reagent arms with different motion trajectories.

[0023] The operation mode of the reaction disc is that the reaction disc is additionally moved forward by one or more than one detection cup position after each rotation cycle, and then paused; during the pause, all reagent needles, sample needles and stirring devices synchronously complete reagent dispensing, sample adding and stirring mixing operations on multiple detection cups on each detection cup belt in parallel; then the reaction disc is again stepped by one or more than one detection cup position, and then paused again, during the pause, all reagent needles, sample needles and stirring devices synchronously complete reagent dispensing, sample adding and stirring mixing operations on multiple detection cups on each detection cup belt in parallel; then the reaction disc is rotated again by one cycle and the same one or more than one detection cup position is added, and then paused, and then stepped forward by one or more than one detection cup position and paused again, and the above operation is continued to be repeated until the detection is completed.

[0024] The reagent dispensing specifically includes that each of the two first reagent arms carries a first reagent needle to suck one or more portions of reagent from a corresponding reagent bottle, and the two first reagent arms move along their respective trajectories, and the trajectories of the two first reagent needles each have one or more overlapping points with one or more detection cups on the reaction disc, and when the reaction disc is stopped, the two first reagent needles each add one portion of reagent to one or more detection cups through the connected diluter at the overlapping point, and after the first dispensing, the reaction disc is displaced forward again to change the trajectories of the two first reagent needles to new detection cups, and the remaining reagent in the first reagent needles of the two first reagent arms is dispensed into one or more new detection cups.

[0025] After the first reagent needles complete the reagent dispensing, they are moved to the corresponding cleaning pool for cleaning, and after the cleaning, the first reagent arms carry the reagent needles to suck one or more portions of reagent from the reagent bottles again to prepare for the next reagent dispensing.

[0026] The sample dispensing specifically includes that when the detection cup added with the first reagent 1 stops at the sample needle, the two sample needles each suck two portions of sample, and the two sample needles add the sucked sample to two different detection cups respectively, and when the reaction disc is displaced forward again, the two sample needles are moved to the corresponding cleaning pool for cleaning, and after the cleaning, the two sample needles each suck another two portions of sample from a different sample tube, and after the reaction disc is displaced forward, the sample needles return to the intersection with the detection cup along their respective trajectories to dispense one portion of sample into two detection cups respectively.

[0027] Or when the reaction disc is stopped, the two sample needles each suck one portion of sample, and the two sample needles are moved to the corresponding detection cup along their respective trajectories to add the sucked sample to the corresponding detection cup respectively, and then the two sample needles each return to the sample tube position where the sample is sucked without cleaning to suck another portion of the same sample and then return to another detection cup position where the sample needle trajectory intersects to dispense another portion of sample again, and then when the reaction disc is displaced forward, the two sample needles are moved to the corresponding cleaning pool for cleaning, and after the cleaning, the two sample needles each suck one or more portions of sample from a different sample tube, and after the reaction disc is displaced forward, the sample needles return to the intersection with the detection cup along their respective trajectories to dispense sample again, and then the sample needles each return to the same sample tube without cleaning to suck the same amount of sample again, and then move to another intersection of the trajectory with the detection cup along the respective trajectory, and add the sucked sample to another detection cup respectively, that is, each sample needle dispenses one or more portions of sample into one or more detection cups when the reaction disc is stopped.

[0028] The reaction disc is rotated again and the sample needles are moved to the cleaning pool for cleaning. After cleaning, the sample needles are moved to the sample sites to prepare for the next sample distribution.

[0029] The stirring is specifically as follows: when a group of detection cups with added reagent 1 and samples reaches the first stirring device during the rotation pause of the reaction disc, if the first stirring device is a non-contact stirring device, the stirring device performs stirring; if the reaction disc moves forward again after the pause, the non-contact stirring device performs stirring again.

[0030] When the first stirring device is a contact stirring device, the first stirring device does not stir during the rotation pause of the reaction disc. After the reaction disc moves forward again, the contact stirring devices of the contact stirring device extend into the inner and outer circle detection cups to perform stirring. After stirring, the stirring device moves to the cleaning pool to clean the contact stirring devices.

[0031] The specific operation of the second reagent distribution according to the detection needs is as follows: if reagent 2 is needed, when a group of detection cups with added reagent 1 and samples and processed by the first stirring device rotates forward to the two second reagent needles, the detection project needs to add reagent 2, and then the two second reagent needles each take one or more portions of reagent 2 and add them to one or more detection cups. Then, according to the forward movement position of the reaction disc, reagent 2 is distributed in the same way as the first reagent needle.

[0032] The second stirring is performed when the reaction disc is paused. If the second stirring device corresponds to detection cups containing reagent 1, reagent 2, and samples, the non-contact stirring device performs stirring on each detection cup. After the reaction disc moves forward after the pause, the non-contact stirring device performs stirring again. When the second stirring device is a contact stirring device, the stirring device does not stir during the rotation pause of the reaction disc. After the reaction disc moves forward again, the contact stirring devices of the contact stirring device extend into the inner and outer circle detection cups to perform stirring. After stirring, the stirring device moves to the cleaning pool to clean the contact stirring devices. Then, the reaction disc rotates forward according to the above rhythm until the detection cups with added reagents and samples complete the detection and enter the multi-stage cleaning device for cleaning. The cleaned detection cups perform detection according to the above process.

[0033] Further, if the device detects that the reagent required to be aspirated by any reagent needle is the same as the reagent previously aspirated, the reagent needle does not need to be cleaned and can directly aspirate the same reagent; if the device detects that the sample required to be aspirated by any sample needle is the same as the sample previously aspirated, the sample needle does not need to be cleaned and can directly aspirate the same sample.

[0034] Further, the mode of the reaction disc advancing in an effective operation cycle of the device is one of the following: the reaction disc rotates one cycle plus one or more detection cup positions; the reaction disc rotates 1 / 2 cycle plus one or more detection cup positions; the reaction disc rotates 1 / 3 cycle plus one or more detection cup positions; and the reaction disc rotates 1 / 8 cycle plus one or more detection cup positions.

[0035] Or, the reaction disc can also rotate one cycle plus one or more detection cup positions plus one or more forward step shifts, and then the reaction disc performs one or more step shifts of the detection cup positions; then the reaction disc rotates one cycle plus one or more detection cup positions, pauses, and then performs one or more step shifts of the detection cup positions.

[0036] Further, the reagent and sample dispensing mode is:

[0037] The two first reagent arms and the two first reagent needles carried thereby are respectively dedicated to dispensing reagents to the inner or outer detection cup belt, and each dispenses two portions of reagent each time. The two second reagent arms and the two second reagent needles carried thereby are respectively dedicated to dispensing reagents to the inner or outer detection cup belt, and each dispenses two portions of reagent each time.

[0038] The two sample arms and the two sample needles carried thereby are respectively responsible for dispensing two portions of one sample each time, and each adds one portion of sample to one detection cup in the inner and outer detection cup belts respectively, and the dispensing of the reagent and the sample is in an interlaced relationship; or, each sample needle first aspirates and dispenses one portion of sample to one detection cup, and then moves to the same sample tube without cleaning to aspirate the same sample and dispense it to another detection cup on the movement track.

[0039] Further, the reagent and sample dispensing mode is:

[0040] The two first reagent arms and the two first reagent needles carried thereby are respectively dedicated to dispensing reagents to the inner or outer detection cup belt, and each dispenses two portions of reagent each time. The two second reagent arms and the two second reagent needles carried thereby are respectively dedicated to dispensing reagents to the inner or outer detection cup belt, and each dispenses two portions of reagent each time.

[0041] The two second reagent arms and their respective second reagent needles each draw two portions of one reagent, and each respectively dispense one portion of the reagent into each of the inner and outer circle detection cup strips, and each of the two second reagent needles dispenses a total of four portions of reagent each time.

[0042] The two sample arms and their respective sample needles each draw two portions of the same sample each time, and the two portions of the same sample drawn by one sample needle are respectively dispensed into one or more different detection cups of only the inner circle, or the outer circle detection cup strip, and the two portions of the same sample drawn by the other sample needle are respectively dispensed into one or more different detection cups of the other circle, in a cross-dispensing relationship with the reagent dispensing, or each sample needle draws one portion of the sample each time, and after dispensing, each draws the same sample again without cleaning, and then returns along its respective track to another detection cup at the intersection, and respectively dispenses the sample into two detection cups, and as needed, the sample needle is used to add an appropriate amount of pure water to the detection cup to ensure complete dispensing of the sample. In this process, one sample needle dispenses one portion of the sample into two different detection cups of the inner circle detection cup strip on the reaction disc, and the other sample needle dispenses one portion of the sample into two different detection cups of the outer circle detection cup strip on the reaction disc, and the sample dispensing is in a cross-dispensing relationship with the reagent dispensing.

[0043] That is, each time the reaction disc is paused, the reagent needles dispense reagent into one or more detection cups of the same circle, and the sample needles dispense sample into detection cups of two different circles, or vice versa, the reagent needles dispense reagent into detection cups of two different circles, and the sample needles dispense sample into detection cups of the same circle. In summary, the same sample is used to perform different reagent item tests, rather than repeating the same test, during normal operation of the device to perform biochemical detection processes.

[0044] Further, the reagent and sample dispensing modes are as follows:

[0045] The two first reagent arms and their respective first reagent needles are respectively dedicated to dispensing reagent into the inner circle or outer circle detection cup strip, and each dispenses two portions of reagent each time; the two second reagent arms and their respective second reagent needles are respectively dedicated to dispensing reagent into the inner circle or outer circle detection cup strip, and each dispenses two portions of reagent each time.

[0046] The two sample arms and their respective sample needles each perform two dispersions of one sample each time, and respectively add sample into one detection cup of the inner circle and one detection cup of the outer circle, and are in a cross-dispensing relationship with the reagent dispensing; and the device limits some detection items to be performed only in the inner circle detection cup, and the remaining items are limited to be detected only in the outer circle detection cup.

[0047] Further, when the device detects that the samples do not detect the same reagent item, or the detection operation conflict cannot be synchronized to implement the same item detection of two different samples simultaneously, the device coordinates the device reagent arm to take one reagent at a time and add it to the required detection cup.

[0048] The device is provided with more than one stirring device, and each stirring device has the function of simultaneously stirring the reagent sample liquid in one and more than one detection cup, and the stirring device is arranged along the periphery of the reaction disc.

[0049] When the instrument is running, the reagent in the reagent bottle on the reagent loading device is quantitatively taken by the reagent needle and transferred to the detection cup on the reaction disc; the sample in each sample tube on the sample loading device is quantitatively taken by the sample needle and transferred to the detection cup.

[0050] The high-speed low-cross-contamination fully automatic biochemical detection method is characterized in that, when the device detects that the samples do not detect the same reagent item, or the detection operation conflict cannot be synchronized to implement the same item detection of two different samples simultaneously, the device coordinates the device reagent arm to take one reagent at a time and add it to the required detection cup; the reagent needle and the sample needle distribute the reagent and the sample in the same detection cup ring every time.

[0051] Further, the two first reagent needles perform specialized division of labor, one of which is only responsible for taking and distributing part of the reagent items, and the other is only responsible for taking and distributing another type of reagent items; the two second reagent needles perform specialized division of labor, one of which is only responsible for taking and distributing part of the reagent items, and the other is only responsible for taking and distributing another type of reagent items; the device performs zoned detection of detection items, part of the detection items are only performed in the inner circle detection cup, and the rest of the detection items are only performed in the outer circle detection cup. Through the above-mentioned specialized division of labor of the reagent needles and the zoned setting of the detection items, the types of reagents contacted by each reagent needle and the combinations of reagents contacted by each detection cup are significantly reduced, thereby greatly reducing the risk of cross-contamination.

[0052] Further, the device is provided with more than one stirring device, which has the function of simultaneously stirring one and more than one detection cup in the inner and outer circle detection cup belt, and the stirring device is of symmetrical structure or of asymmetrical structure.

[0053] Further, the two first reagent arms and their respective first reagent needles perform reagent distribution on adjacent detection cups in the inner and outer circle detection cup belt, respectively, or one of the two first reagent needles only distributes reagents on adjacent different detection cups in the inner circle detection cup belt; the other first reagent needle only distributes reagents on adjacent different detection cups in the outer circle detection cup belt.

[0054] Corresponding to the two sample needles, only the adjacent detection cups on each cup belt in the inner and outer two detection cup belts are allocated samples;

[0055] Corresponding to the two second reagent arms and their respective second reagent needles, reagent allocation is performed on adjacent detection cups on different detection cup belts in the inner and outer rings, or one of the two second reagent needles only allocates reagent 2 to adjacent different detection cups on the inner ring detection cup belt; the other second reagent needle only allocates reagent 2 to adjacent different detection cups on the outer ring detection cup belt;

[0056] Or each first reagent needle, each sample needle, and each second reagent needle only allocates reagent, sample to more than one non-adjacent detection cup on one of the inner and outer two different ring detection cup belts.

[0057] Advantages:

[0058] The high-speed low-cross-contamination fully-automatic biochemical detection device designed by the application uses one reagent needle carried by one reagent arm to absorb more than one reagent at a time, and then allocates the reagent to multiple different detection cups at a time, which greatly saves the time consumed by the reagent needle for absorbing and allocating reagent, and effectively improves the running speed of the device.

[0059] One reagent arm can replace the function of two or more reagent arms in similar devices, greatly simplifying the structure of the device and reducing the manufacturing cost of the device.

[0060] Since the number of times of absorbing reagent from the reagent bottle by the reagent needle is reduced, the probability of residual reagent, water, etc. carried by the reagent needle entering the reagent bottle is greatly reduced, the pollution of the reagent in the reagent bottle by the reagent needle when absorbing reagent is effectively reduced, and the stability, quality, and detection result quality of the reagent can be better guaranteed.

[0061] After absorbing reagent each time, the reagent needle needs to be cleaned inside and outside, and the residual inside the reagent needle is the main source of cross-contamination of the fully-automatic biochemical analyzer. The high-speed low-cross-contamination fully-automatic biochemical detection device designed by the application absorbs multiple reagents at a time and allocates the reagents to more than one detection cup, and in addition, the device can limit each reagent needle to only contact 1 / 2 or 1 / 4 types of reagents, and can also limit the inner and outer ring detection cup belts to only perform 1 / 2 reagent item detection, respectively. Such optimized process can greatly reduce the types of reagents contacted by each reagent needle, and also reduce the types of reagents contacted by the detection cup, so the probability of the reagent needle and the detection cup contacting different reagents is greatly reduced, the probability of cross-contamination is reduced, and the number of times of cleaning the reagent needle is reduced. While reducing cross-contamination, the water consumption and wastewater generation of the device are also greatly reduced, and the device is more environmentally friendly.

[0062] In summary, the high-speed low-cross-contamination full-automatic biochemical detection device designed with less components can meet the speed performance of other full-automatic biochemical detection device design, and the reagent needle cleaning amount of the device is greatly reduced, the cross-contamination is reduced, the detection accuracy of the device is improved, the stability of the reagent is improved, the waste water generated by the device is less, and the device structure is simpler and faster. The design of the design scheme effectively improves the performance of the full-automatic biochemical detection device in many aspects, which is beneficial to the comprehensive technical performance of the full-automatic biochemical detection device. The design scheme of the application is also applicable to full-automatic immune analyzers, full-automatic blood clotting analyzers and the like. BRIEF DESCRIPTION OF DRAWINGS

[0063] Figure 1 The main structure relationship diagram of a high-speed low-cross-contamination full-automatic biochemical detection device is shown.

[0064] Figure 2 The main structure relationship diagram of another embodiment of a high-speed low-cross-contamination full-automatic biochemical detection device is shown.

[0065] The reference signs are as follows: 1-reaction disc; 2-first detection cup belt; 3-second detection cup belt; 4-first reagent loading device; 5-first reagent arm; 6-first reagent needle cleaning pool; 7-first sample arm; 8-first sample needle cleaning pool; 9-second sample arm; 10-second sample needle cleaning pool; 11-first stirring device; 12-first stirring device cleaning pool; 14-second reagent arm; 15-second reagent needle cleaning pool; 16-second reagent loading device; 17-second stirring device; 18-second stirring device cleaning pool; 20-multistage cleaning device; 21-sample loading device; 22-optical detection unit; 23-first multi-head stirring device; 24-first multi-head stirring device cleaning pool; 25-second multi-head stirring device; 26-second multi-head stirring device cleaning pool. DETAILED DESCRIPTION

[0066] Example 1

[0067] Reference is made to the accompanying drawings Figure 1The device is shown as a high-speed low-cross contamination automatic biochemical detection device. The main structure of the device includes two reagent loading devices, which are a first reagent loading device 4 and a second reagent loading device 16. A reaction disc 1 is loaded with two circular ring-shaped parallel equidistant uniform detection cup strips, which are a first detection cup strip 2 and a second detection cup strip 3. Each detection cup ring strip is loaded with more than one detection cup. A sample loading device 21 can be in the form of a track or one or more than one disc. Two stirring devices for stirring the sample reagent liquid in the detection cup are provided, which are a first stirring device 11 and a second stirring device 17. An optical detection unit 22 is provided for performing detection on the inner and outer two layers of detection cups loaded on the reaction disc. A cleaning device 20 is provided for multi-stage cleaning of the detection cup. The device also has a second reagent arm 14, which has a second reagent needle and a second reagent needle cleaning pool 15 for cleaning the second reagent needle. The device has two sample arms, which are a first sample arm 7 and a second sample arm 9. Each sample arm has a sample needle for quantitative sampling and dispensing. The device also has a cleaning pool for each sample needle, which are a first sample needle cleaning pool 8 and a second sample needle cleaning pool 10. The device also has a first stirring device 11 with two asymmetric stirring needles, and a first stirring device cleaning pool 12 is provided for each stirring needle of the first stirring device. The device also has a second stirring device 17, which is provided with a second stirring device cleaning pool 18, and at least one multi-stage cleaning device 20 is provided for multi-stage cleaning of the detection cup after detection.

[0068] The detection workflow of the device designed according to the scheme is as follows:

[0069] After the reaction disc 1 rotates one full circle and pauses at a reagent position, a first reagent arm 5 of the device has previously taken two reagents and dispenses one reagent to each of the two detection cup positions on its track. The detection cups on the track are located on the first detection cup strip 2 and the second detection cup strip 3. Then the first reagent arm 5 carries the reagent needle to the first reagent needle cleaning pool 6 for cleaning, and then to the first reagent loading device 4 to take two reagents for the next reagent dispensing.

[0070] The reaction disc continues to advance in a one full circle plus one reagent position mode. When the detection cup with reagent 1 is rotated to the sample needle track and pauses, the two sample needles, i.e., the first sample needle and the second sample needle, add the samples they have taken to the two detection cups, respectively. The two detection cups correspond to the two reagent cups with the same reagent as reagent 1. Then the two sample needles move to their respective cleaning pools to clean the two sample needles. After cleaning, the sample needles move to the sample loading device 21 to take one sample each, waiting for the next sample dispensing.

[0071] The reaction disc continues to make one whole circle and advances by one reagent position. When the detection cup to which reagent 1 and sample have been added rotates to the first stirring device 11 position, the reaction disc pauses, the first stirring device 1 moves along its own track to the two detection cup positions, and stirring is performed in the two detection cups to which reagent 1 and sample have been added. Then the first stirring device 11 carries the two stirring needles along their respective tracks to the cleaning pool 12 to perform cleaning on the two stirring needles. After cleaning, the stirring needles are ready for the next stirring.

[0072] The stirring of the full-automatic biochemical detection device designed in the present application can adopt a contact stirring mode such as a stirring needle, including a single paddle, a double paddle, and a triangular angle distribution of three groups of stirring needles, an injection needle suction and impact mode, etc., or a non-contact stirring mode such as a non-uniform air pressure stirring, an ultrasonic stirring, etc.

[0073] The reaction disc continues to make one whole circle and advances by one reagent position. When the detection cup to which reagent 1 and sample have been added rotates to the first stirring device 11 position, the reaction disc pauses, the first stirring device 1 moves along its own track to the two detection cup positions, and stirring is performed in the two detection cups to which reagent 1 and sample have been added. Then the first stirring device 11 carries the two stirring needles along their respective tracks to the cleaning pool 12 to perform cleaning on the two stirring needles. After cleaning, the stirring needles are ready for the next stirring.

[0074] The reaction disc continues to make one whole circle and advances by one reagent position. When the detection cup to which reagent 1 and sample have been added rotates to the first stirring device 11 position, the reaction disc pauses, the first stirring device 1 moves along its own track to the two detection cup positions, and stirring is performed in the two detection cups to which reagent 1 and sample have been added. Then the first stirring device 11 carries the two stirring needles along their respective tracks to the cleaning pool 12 to perform cleaning on the two stirring needles. After cleaning, the stirring needles are ready for the next stirring.

[0075] The reaction disc is also provided with a light signal detection device 22, which synchronously detects each detection cup arranged in the inner and outer circles of the reaction disc. Each detection cup receives detection once when it passes through the detection device each time due to the cyclic rotation of the reaction disc. That is, each detection cup receives reagent 1, sample, stirring, reagent 2, stirring, and detection multiple times during the operation of the detection cup.

[0076] After all the tests are completed, the test cups enter the multi-stage cleaning device 20, where the reaction liquid in the test cups is completely removed and multiple cleaning is performed. The test cups after cleaning are used again for new sample and reagent testing until all samples are tested.

[0077] In the design, the reagent needle can suck 2 portions of reagent each time, or more than 2 portions of reagent each time, i.e., the reagent sucked by the reagent needle each time can be distributed to more than 2 test cups, and then the reagent needle is cleaned to suck new reagent. In this embodiment, the stirring needles of the stirring device are arranged in an asymmetric distribution, which is suitable for stirring multiple test cups at the same time.

[0078] Embodiment 2

[0079] As shown in the accompanying drawings Figure 2 Another embodiment of the design is different from the device structure of embodiment 1 in that the device has four reagent arms, two first reagent arms each with a corresponding cleaning pool, two second reagent arms each with a corresponding cleaning pool, a first multi-head stirring device 23 instead of the first stirring device 11, a first multi-head stirring device cleaning pool 24 instead of the first stirring device cleaning pool 12, a second multi-head stirring device 25 instead of the second stirring device 18, and a second multi-head stirring device cleaning pool 26 instead of the first stirring device cleaning pool 19. In this design, the first multi-head stirring device 23 and the second multi-head stirring device 25 each have 4 more stirring needles.

[0080] The main working process of the fully automatic biochemical testing device according to the design is as follows: the reaction disc of the device rotates forward by 2 test cup positions per rotation, and when the reaction disc stops, the two first reagent arms 1 each suck two equal portions of reagent from the same reagent bottle with the reagent needles carried by the two first reagent arms. After sucking the reagent, the first reagent needles carried by the two first reagent arms move along their respective trajectories to the two test cups intersected by the two first reagent arms, respectively, and each of the two reagent needles distributes one portion of the sucked reagent to the two test cups. Then the two first reagent arms carrying the reagent needles move to the cleaning pool to perform cleaning, and after cleaning, new reagent is sucked.

[0081] Subsequently, the reaction disc continues to rotate in the mode of one rotation per two detection cups to reach the sample loading position pause, and one sample needle carried by the first sample arm 7 and one sample needle carried by the second sample arm 9 have respectively taken one sample from two different sample tubes in advance, the two sample needles are located at different tracks and each sample needle intersects with two detection cup positions on the device reaction disc, and the four detection cups at the intersection point have been respectively added with reagent 1 in advance, the sample needles respectively reach one detection cup along the respective track to add one sample, and then the first sample arm 7 and the second sample arm 9 respectively move the sample needles to the respective cleaning pool to perform cleaning, and then respectively take one sample from two different sample tubes on the sample loading device 21, and then move along the respective track to two detection cup positions without sample to add two samples, and then the first sample arm 7 and the second sample arm 9 respectively move the sample needles to the respective cleaning pool to perform cleaning, and then respectively take one sample from two different sample tubes on the sample loading device 21 to be added to the subsequently moving detection cup.

[0082] When the reaction disc continues to rotate in the previous mode, when the detection cup reaches the first stirring position pause after adding reagent 1 and sample, the first multi-head stirring device 23 carries four stirring needles, and the four stirring needles simultaneously stir two inner detection cups and two outer detection cups on the reaction disc.

[0083] When the reaction disc continues to rotate in the previous mode, when the detection cup reaches the second reagent arm, if reagent 2 is needed, the two second reagent arms of the device also pre-take two equal amounts of the same reagent 2, and when the reaction disc pauses, each two detection cups on the inner and outer sides of the reaction disc are respectively added with equal amounts of reagent 2.

[0084] When the reaction disc continues to rotate in the same mode as above, when the reaction disc pauses again, the detection cups that have been added with reagent 1 and sample and stirred reach the second multi-head stirring device 25 position on the reaction disc, and the second multi-head stirring device moves to the corresponding detection cup position to perform stirring on the liquid in the corresponding four detection cups, and the process is the same as the working process of the first multi-head stirring device.

[0085] A light signal detection device is also provided beside the reaction disc of the device to synchronously detect the detection cups arranged in the inner and outer circles of the reaction disc, and each detection cup receives detection once when passing through the detection device each time due to the cyclic rotation of the reaction disc. That is, each detection cup receives detection multiple times in the subsequent detection cup operation after receiving reagent 1, adding sample, stirring, adding second reagent, and stirring, until the detection cup completes detection and enters the multi-stage cleaning device to remove the reaction liquid in the detection cup and perform multiple cleaning. The cleaned detection cup is used for new detection again.

[0086] In the device process of the present design, the effective operation cycle of the device is: after the reaction disc completes one cycle plus one or more detection cup displacements, it is paused, and during the pause of the reaction disc, the reagent needle, sample needle, and stirring device needle perform corresponding processing on an equal number of detection cups with corresponding positions, and then the reaction disc moves again for one or more positions, and the distance of the movement is equal to the increased detection cup position when the reaction disc completes one cycle of rotation. After each reaction cup displacement and pause, the reagent needle, sample needle, and stirring device needle perform corresponding processing on an equal number of detection cups with corresponding positions until the end.

[0087] In the device process of the present design, the effective operation cycle of the device is: after the reaction disc completes one cycle plus one or more detection cup displacements, it is paused, and during the pause of the reaction disc, the reagent needle, sample needle, and stirring device needle perform corresponding processing on an equal number of detection cups with corresponding positions, and then the reaction disc moves again for one or more positions, and the distance of the movement is equal to the increased detection cup position when the reaction disc completes one cycle of rotation. After each reaction cup displacement and pause, the reagent needle, sample needle, and stirring device needle perform corresponding processing on an equal number of detection cups with corresponding positions until the end.

[0088] In the device process of the present design, the effective operation cycle of the device is: after the reaction disc completes one cycle plus one or more detection cup displacements, it is paused, and during the pause of the reaction disc, the reagent needle, sample needle, and stirring device needle perform corresponding processing on an equal number of detection cups with corresponding positions, and then the reaction disc moves again for one or more positions, and the distance of the movement is equal to the increased detection cup position when the reaction disc completes one cycle of rotation. After each reaction cup displacement and pause, the reagent needle, sample needle, and stirring device needle perform corresponding processing on an equal number of detection cups with corresponding positions until the end.

[0089] In the device process of the present design, the first reagent needle and the second reagent needle can each take 2 or more reagents at a time and distribute them into 2 or more detection cups. The corresponding stirring device, sample distribution device of the device processes an appropriate number of reaction cups in each effective operation cycle of the device, and the speed potential of the device is further improved.

[0090] In the device process of the present design, the effective operation cycle of the device is: after the reaction disc completes one cycle plus one or more detection cup displacements, it is paused, and during the pause of the reaction disc, the reagent needle, sample needle, and stirring device needle perform corresponding processing on an equal number of detection cups with corresponding positions, and then the reaction disc moves again for one or more positions, and the distance of the movement is equal to the increased detection cup position when the reaction disc completes one cycle of rotation. After each reaction cup displacement and pause, the reagent needle, sample needle, and stirring device needle perform corresponding processing on an equal number of detection cups with corresponding positions until the end.

[0091] Example 3

[0092] The difference between the above examples is that the first reagent needle and the second reagent needle of the device can each take 4 reagents at a time and add them into four detection cups in four times.

[0093] Example 4

[0094] The rotation cycle of the device reaction disc can be rotated forward in the manner of one complete cycle plus one or more detection cup positions, or 1 / 2 cycle plus one detection cup position.

[0095] Example 5

[0096] The device sample disc is disc type, the device sets group detection, that is, one group detects the same item, and the detection number of the same item detection group is more than one; in one detection group, each reagent needle only absorbs and distributes one kind of reagent, each reagent needle is not cleaned when absorbing and distributing each same reagent, each reagent needle is cleaned before absorbing and distributing different reagents, and then continuously absorbs and distributes the new reagent until the next new reagent is changed to absorb and distribute, and then cleaned. During the period of absorbing and distributing the same group of reagents by each reagent needle, the sample needle respectively absorbs and distributes different samples, and the sample needle is cleaned before and after absorbing and distributing each different sample. The stirring device is a contact type stirring needle device, and each stirring needle is cleaned before and after each stirring. The mode can effectively reduce the degree of cross contamination, and has more advantages in water resource consumption.

[0097] The application provides a high-speed low-cross-contamination full-automatic biochemical detection device and method, and the technical scheme is implemented in many ways. The above description is only the preferred embodiment of the application. It should be pointed out that the ordinary skilled in the art can make some improvements and refinements without departing from the principle of the application. These improvements and refinements should also be considered as the protection scope of the application. The components not mentioned in the embodiment can be realized by the existing technology.

Claims

1. A high-speed, low-cross-contamination fully automated biochemical detection device, characterized in that, The device includes a reaction disk, at least one sample loading device, and at least one reagent loading device; the device has one or more first reagent arms, one or more second reagent arms, and one or more sample arms, each reagent arm having a reagent needle for quantitative aspiration and dispensing of reagents; each sample arm having a sample needle for quantitative aspiration and dispensing of samples. The reaction disk and the reagent loading device are connected by reagent needles loaded on the reagent arm for quantitative transfer of reagents, and the reaction disk and the sample loading device are connected by sample needles carried on the sample arm for quantitative transfer of samples. The device is also equipped with cleaning devices for cleaning each reagent needle and each sample needle. The reaction disk is surrounded by one or more concentric circular arrays of test cups with equal circumferential spacing. Each test cup array has one or more test cups. Along the movement trajectory of the test cup arrays, there are multi-stage test cup cleaning devices and optical detection units. Each device has one or more stirring devices, and each stirring device has the function of simultaneously mixing the reagent sample solution in one or more test cups on different test cup arrays. The stirring devices are arranged around the periphery of the reaction disk. During instrument operation, the reagents in the reagent bottles on the reagent loading device are quantitatively drawn and transferred to the test cups on the reaction plate by the reagent needle; the samples in each sample tube on the sample loading device are quantitatively drawn and transferred to the test cups by the sample needle.

2. The high-speed, low-cross-contamination fully automated biochemical detection device according to claim 1, characterized in that, The reagent loading device includes a first reagent loading device and a second reagent loading device. The first reagent loading device quantitatively transfers reagents to the reaction disk through a first reagent needle carried by a first reagent arm. The second reagent loading device quantitatively transfers reagents to the reaction disk through a second reagent needle carried by a second reagent arm. Each of the first and second reagent arms has a reagent needle at one end. The reaction disk has two rings of detection cups. The running trajectory of each reagent needle intersects both rings of detection cups on the reaction disk, or intersects one of the rings of detection cups. The sample arm includes a first sample arm and a second sample arm. Each of the first and second sample arms is provided with a sample needle at one end. The running trajectory of the sample needle intersects both of the two detection cup bands of the reaction disk; or each sample needle intersects only one of the two detection cup bands of the reaction disk. The stirring device includes a first stirring device and a second stirring device, which can simultaneously stir one or more detection cups on each of the two rings of detection cups on the reaction plate. When adding reagents or samples consecutively, reagents or samples can be added to adjacent test cups in the same test cup ring; or they can be added to non-adjacent test cups in the same test cup ring.

3. A high-speed, low-cross-contamination fully automated biochemical detection method, characterized in that, The biochemical detection device applied to any one of claims 1-2 specifically includes: The reaction disk operates in a regular pattern: it rotates once and then pauses after adding at least one more test cup position. During the pause, all reagent needles, sample needles, and stirring devices simultaneously perform reagent dispensing, sample dispensing, and mixing operations on one or more test cups in the two-ring test cup series. The reagent dispensing process is as follows: When the reaction disk of the device rotates forward, the two first reagent arms of the device draw two portions of reagent 1 from different reagent bottles on the first reagent loading device through their respective first reagent needles; when the reaction disk pauses according to the prescribed operating mode, the two first reagent needles add one portion of reagent to each of the two detection cups in the two rings of detection cups; when the reaction disk rotates forward, after each of the two first reagent arms draws two portions of reagent, it moves to the intersection point of one detection cup on the reaction disk; when the reaction disk stops rotating, one detection cup of the detection cups stops directly below one first reagent needle, and the other detection cup stops below another first reagent needle 1; the two first reagent needles add one portion of reagent to each detection cup through the connected diluent; then the two first reagent needles move to the intersection point of each of the two detection cups on the reaction disk, and each of the two first reagent needles adds its remaining portion of reagent to a detection cup located below its respective needle; that is, the two first reagent needles complete the addition of one portion of reagent to each of four detection cups in one operation, and the two first reagent needles add different reagents; After the dispensing is completed, the two first reagent arms, each carrying its own first reagent needle, move to the respective cleaning pools for cleaning. After cleaning, the two first reagent arms each draw two portions of reagent from different reagent bottles using their respective reagent needles, ready to perform the next reagent dispensing. The sample allocation process is as follows: When the reaction disk rotates to the sample addition position and pauses, each sample arm's sample needle has pre-absorbed two samples. At this time, one pre-absorbed sample is added to a test cup containing reagent 1 through the connected diluent, and the other sample is added to another test cup containing a different reagent 1; or each sample needle adds one pre-absorbed sample to a test cup, and then, after cleaning or without cleaning, returns to the original sample tube from which the sample was aspirated, and then aspirates the same sample again and adds it to a different test cup; that is, during each pause of the reaction disk, two sample needles complete the addition of one sample to each of the four test cups, and during this process, each sample needle adds a different sample, and the sample aspirated by each sample needle is the same as the previous sample; after the sample allocation is completed, the sample needles carried by the sample arm are moved to the cleaning tank for cleaning. After cleaning, the sample arm aspirates the sample again through the sample needle to the sample tube to prepare for the next sample allocation. Specifically, when the reaction disk rotates forward and then pauses, the first stirring device stirs one or more test cups containing reagent 1 and sample on different test cup belts below it. The first stirring device simultaneously stirs one or more test cups located on each of the two test cup belts, so that the reagent 1 and sample in each stirred test cup are fully mixed and reacted. A second reagent dispensing is performed according to the testing requirements: When adding reagent 2 according to the testing requirements, each second reagent needle pre-draws two portions of reagent 2. When the reaction plate rotates forward and pauses, each second reagent needle adds one portion of reagent 2 to each of the two test cups that have already been filled with reagent 1, the sample, and the stirred test cup. The two portions of reagent 2 added by one second reagent needle correspond to the two portions of reagent added by one first reagent needle in the test cup, and the two portions of reagent added by the other second reagent needle correspond to the two portions of reagent added by the other first reagent needle in the test cup. That is, the two second reagent needles complete the addition of reagents to four test cups at one time, and each of the two second reagent needles adds a different reagent. The specific operation of the second stirring is as follows: when the reaction plate rotates to a stop, if the detection cup below the second stirring device contains reagent 1, reagent 2 and sample, the second stirring device simultaneously stirs one or more detection cups located on each of the two detection cup belts, so that reagent 1, reagent 2 and sample in the cup are fully mixed and reacted. The optical detection and cleaning process includes the optical signal detection unit synchronously performing detection on each detection cup set on each detection cup belt of the reaction plate. That is, each detection cup is detected once after receiving reagent 1, adding sample and stirring, adding second reagent and stirring, and each detection cup is detected once when it passes through the optical signal detection unit during each operation before and after the reaction plate, until each detection cup completes the detection and enters the multi-stage cleaning device. In the multi-stage cleaning device, the reaction liquid in the detection cup is removed and multiple cleanings are performed. After cleaning, the detection cup is recycled for detection until all the required samples have been detected.

4. The high-speed, low-cross-contamination fully automated biochemical detection method according to claim 3, characterized in that, The device includes a first reagent arm with two different motion trajectories and a second reagent arm with two different motion trajectories; The reaction disk operates as follows: After each complete rotation, the reaction disk adds one or more detection cup positions and then pauses. During this pause, all reagent needles, sample needles, and stirring devices simultaneously perform reagent dispensing, sample addition, and mixing operations on multiple detection cups along each detection cup belt. The reaction disk then advances to one or more detection cup positions, pauses again, and during this pause, all reagent needles, sample needles, and stirring devices simultaneously perform reagent dispensing, sample addition, and mixing operations on multiple detection cups along each advancing detection cup belt. The reaction disk then rotates one more revolution, adds one or more of the same detection cup positions, pauses again, advances to one or more detection cup positions, pauses again, and continues this cycle until the detection is complete. The reagent dispensing process is as follows: each of the two first reagent arms of the device carries a first reagent needle, which draws more than one part of the reagent from a different reagent bottle. The two first reagent arms, each carrying a first reagent needle, move along their respective tracks. The tracks of the two first reagent needles coincide with one or more detection cups on each detection cup ring on the reaction plate. When the reaction plate stops rotating, the two first reagent needles, through their connected diluents, add equal amounts of reagent to one or more detection cups at the points where their tracks coincide. After the initial dispensing is completed, the reaction plate moves forward again, causing the tracks of the two first reagent needles to change to new detection cups. The remaining reagent in the first reagent needles carried by the two first reagent arms is then dispensed into one or more new detection cups. After each first reagent needle completes reagent dispensing, it moves to its respective cleaning tank for cleaning. After cleaning, each first reagent arm moves the reagent needle to draw up more than one reagent from the reagent bottle, ready to perform the next reagent dispensing. The sample allocation is as follows: when the test cup containing reagent 1, added via the first reagent needle, stops at the sample needle, each of the two sample needles has already drawn two samples. One of the sample needles adds the two drawn samples into two different test cups respectively. When the reaction plate moves forward, the other sample needle allocates one sample to each of the other two test cups. Subsequently, the two sample needles move to their respective cleaning tanks for cleaning. After cleaning, the two sample needles each draw two more samples into a different sample tube. After the reaction plate moves forward, the sample needles return to the intersection point with the test cups along their respective trajectories, and then each allocates one sample to each of the two test cups. Alternatively, when the reaction plate pauses, each of the two sample needles has already drawn a sample. They then follow their respective needle paths to the detection cup where their paths intersect with the detection cup, adding their drawn samples to their respective cups. Afterward, without cleaning, each needle returns to its original sample tube position, draws another sample, and then returns to the other detection cup where their paths intersects, dispensing another sample. Subsequently, when the reaction plate moves forward in a stepping motion, the two needles move to their respective cleaning tanks for cleaning. After cleaning, the two needles are then... Each sample needle draws a sample from a different sample tube. After the reaction plate moves forward, the sample needles return to their respective paths and intersect with the detection cups to distribute the sample. Then, without cleaning, each sample needle returns to the same sample tube and draws an equal amount of sample. Afterward, each sample needle moves to another intersection point with the detection cup and adds the drawn sample to another detection cup. That is, each sample needle distributes more than one sample to more than one detection cup when the reaction plate stops rotating. After the reaction plate moves forward, each sample needle distributes a sample again. The reaction disk restarts its cycle rotation and increments the position forward. After each sample needle completes sample aspiration and distribution, it moves to its respective cleaning pool for cleaning. After cleaning, each sample arm drives each sample needle to the sample position to aspirate the sample again, ready to perform the next sample distribution. The stirring is specifically as follows: when the reaction disk stops rotating, a set of test cups containing reagent 1 and sample arrives at the first stirring device. If the first stirring device is a non-contact stirring device, the stirring device will perform stirring. If the reaction disk moves forward again after stopping, the non-contact stirring device will perform stirring again. When the first stirring device is a contact stirring device, the first stirring device will not stir temporarily when the reaction plate stops rotating. After the reaction plate moves forward again, each contact stirring needle of the contact stirring device will simultaneously extend into one or more detection cups in the inner and outer rings to perform stirring. After stirring is completed, the stirring device will be moved to the cleaning tank to clean each contact stirring device. The specific operation of dispensing the second reagent according to the testing needs is as follows: If reagent 2 needs to be added, when a set of test cups that have been treated by the first stirring device after adding reagent 1 and sample rotates forward to the two second reagent needles, if the test item requires the addition of reagent 2, then each of the two second reagent needles will draw more than one part of reagent 2 and add one part of reagent to each of the test cups; and according to the step-forward position of the reaction plate, the dispensing of reagent 2 will be increased in accordance with the pattern of the first reagent needle; When the reaction plate stops rotating, if the test cup corresponding to the second stirring device contains reagent 1, reagent 2 and sample, and if the second stirring device is non-contact, the second stirring device will stir each test cup it faces. After the reaction plate moves forward after the pause, the non-contact stirring device will stir again. When the second stirring device is a contact stirring device, the stirring device will not stir when the reaction plate stops rotating. After the reaction plate stops and then moves forward again, each contact stirring needle of the contact stirring device will extend into one or more detection cups located on the inner and outer sides of the detection cup belt to be stirred, and stir simultaneously. After stirring is completed, the stirring device will be moved to the cleaning tank to clean each contact stirring device. The reaction disk then continues to rotate forward according to the above rhythm until each test cup that has been treated with reagents and samples enters the multi-stage cleaning device for cleaning. After cleaning, each test cup is then tested in a cycle according to the above process.

5. The high-speed, low-cross-contamination fully automated biochemical detection method according to claim 3 or 4, characterized in that, If, within the same operating cycle or between different operating cycles, the reagent to be drawn by any reagent needle required for detection is the same as the reagent drawn previously, then the reagent needle can directly draw the same reagent without cleaning; if the sample to be drawn by any sample needle required for detection is the same as the sample drawn previously, then the sample needle can directly draw the same sample without cleaning.

6. The high-speed, low-cross-contamination fully automated biochemical detection method according to claim 5, characterized in that, The effective operating cycle of this device allows for the following modes of reaction disk rotation: one rotation of the reaction disk plus one or more detection cup positions; one rotation of the reaction disk plus one or more detection cup positions; one rotation of the reaction disk plus one or more detection cup positions; up to one rotation of the reaction disk plus one or more detection cup positions; and a maximum of one rotation of the reaction disk plus one or more detection cup positions. In addition, after the reaction disk completes one cycle and moves forward by one or more detection cup positions, it can also perform a step-forward movement of 0 or more detection cup positions.

7. The high-speed, low-cross-contamination fully automated biochemical detection method according to claim 4, characterized in that, The reagent and sample distribution pattern is as follows: The two first reagent arms and the two first reagent needles carried by each arm are respectively used to dispense reagents to the inner or outer detection cups, dispensing two portions of reagent each time. Each of the two second reagent arms and its two second reagent needles is dedicated to dispensing reagents to the inner or outer ring detection cup band, dispensing two portions of reagent each time. Each of the two sample arms and their sample needles is responsible for dispensing one type of sample twice during each dispensing process, adding one sample to each of the inner and outer detection cups respectively, and in a cross-coordinated manner with the reagent dispensing; or, during a sample dispensing process, each sample needle first draws and dispenses one sample to one detection cup, and then moves to the same sample tube again without cleaning to draw the same sample, and then dispenses it to another detection cup on its movement trajectory.

8. The high-speed, low-cross-contamination fully automated biochemical detection method according to claim 4, characterized in that, The reagent and sample distribution pattern is as follows: The two first reagent arms and their respective first reagent needles draw up two portions of one reagent at a time and dispense one portion into each of the inner and outer detection cups; while the other first reagent needle draws up two portions of another reagent each time and dispenses one portion into each of the inner and outer detection cups; thus, the two first reagent needles dispense a total of four reagents at a time. The two second reagent arms and their respective second reagent needles also draw two portions of a reagent, just as the two first reagent needles do, and each dispense one portion of the reagent into one detection cup in the inner and outer rings respectively. Each time, the two second reagent needles dispense a total of four portions of reagent. Each of the two sample arms and their respective sample needles draws two portions of the same sample each time. The two portions of the same sample drawn by one sample needle are distributed to two different detection cups on one of the inner and outer detection cup belts, respectively. The two portions of the same sample drawn by the other sample needle are distributed to two different detection cups on the other detection cup belt, which is in a cross-matching relationship with the reagent distribution. Alternatively, each sample needle can draw one sample at a time, and after distribution, without washing, draw the same sample again and then return along their respective paths to the other intersecting test cup to distribute the samples into the two test cups. During distribution, an appropriate amount of pure water can be added to the test cup through the sample needle as needed to ensure complete sample distribution.

9. The high-speed, low-cross-contamination fully automated biochemical detection method according to claim 4, characterized in that, The reagent and sample distribution pattern is as follows: The two first reagent arms and their respective first reagent needles are dedicated to dispensing reagents to the inner or outer detection cups, dispensing two portions of reagent each time. The two second reagent arms and their respective second reagent needles are dedicated to dispensing reagents to the inner or outer detection cups, dispensing two portions of reagent each time. The two sample arms and their respective sample needles each perform two dispensing operations for one type of sample each time, adding the sample to one detection cup in the inner circle and one in the outer circle respectively, and coordinating with the reagent dispensing. Furthermore, the device limits some detection items to be performed only in the inner detection cup, while the remaining items are limited to being performed only in the outer detection cup.

10. The high-speed, low-cross-contamination fully automated biochemical detection method according to claim 9, characterized in that, The two first reagent needles are assigned specialized functions, with one first reagent needle only responsible for drawing and dispensing a portion of the reagents, and the other first reagent needle only responsible for drawing and dispensing the other portion of the reagents. The two second reagent needles are specialized and have different functions. One second reagent needle is only responsible for drawing and dispensing a portion of the reagents, while the other second reagent needle is only responsible for drawing and dispensing the other portion of the reagents. The device performs zoned testing on the test items, with some test items being tested only in the inner ring test cup and the remaining test items being tested only in the outer ring test cup.

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