Use of a vanilla extract in the preparation of a perfume

Abstract

The application discloses application of a vanilla extract in preparation of perfume, and relates to the technical field of vanilla application. The vanilla extract is composed of vanilla extract 1, vanilla extract 2, vanilla extract 3 and vanilla extract 4. The vanilla extract 1 is obtained by using an ethanol low-temperature extraction method. The vanilla extract 2 is obtained by using a supercritical CO2 extraction method. The vanilla extract 3 is obtained by using a grease extraction method. The vanilla extract 4 is obtained by using a water vapor distillation method. The mass fraction ratio of the vanilla extract 1, the vanilla extract 2, the vanilla extract 3 and the vanilla extract 4 is 10-25:70-85:2-8:0.5-3. The vanilla extract is mixed with acceptable auxiliary materials for preparing perfume, and the mixture is uniformly mixed to prepare the perfume. The prepared perfume has the fresh and clear scent of the vanilla when it is just bloomed, the rich sweet scent in the middle section, the light and elegant aftertaste in the tail section, and no burnt taste, sour taste, alcohol residual taste and other miscellaneous tastes.

Description

Technical Field

[0001] This invention relates to the field of epiphyllum application technology, specifically to the application of epiphyllum extract in the preparation of perfume. Background Technology

[0002] Epiphyllum ( Epiphyllum oxypetalum *Epiphyllum oxypetalum* (DC.) Haw. is a perennial succulent plant belonging to the Cactaceae family, native to Mexico and Nicaragua. Due to its short-lived nocturnal blooming and unique visual and olfactory value, it is known as the "Queen of the Night." Not only does it have high ornamental value, but it is also a plant with both medicinal and edible uses. Traditionally, it has been used to treat gastrointestinal ailments. Modern research has further confirmed that its extracts contain moisturizing, whitening, and antioxidant active ingredients, and it has already begun to be used in the cosmetics industry. However, one of the most core values ​​of *Epiphyllum oxypetalum*—its sweet and rich fragrance and the utilization of its volatile organic compounds (VOCs)—has not yet been fully developed, and related technologies have significant shortcomings.

[0003] Current research on the aroma components of Epiphyllum oxypetalum reveals insufficient understanding of the spatiotemporal release patterns of its VOCs. Early studies largely focused on the pharmacological activities and genomic characteristics of Epiphyllum oxypetalum, with very limited exploration of the dynamic changes in its fragrance. Although some studies have attempted to analyze VOCs in Epiphyllum oxypetalum using gas chromatography-related techniques, there are significant technical limitations. For example, while headspace gas chromatography-ion mobility spectrometry (HS-GC-IMS) can quickly obtain compound fingerprints, it cannot distinguish the differences in VOCs at different flowering stages (pre-flowering, semi-open, and full bloom) due to insufficient database coverage and sample pretreatment (such as mechanical damage caused by 40℃ incubation and petal separation). Furthermore, it is prone to detecting C6 green leaf volatiles induced by heat or mechanical damage (such as (E)-2-hexenal), which deviates from the aroma composition of Epiphyllum oxypetalum in its natural state. While headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) can detect more VOCs, it requires mechanical separation of flower organs and liquid nitrogen freezing-thawing treatment, resulting in an excessively high proportion of fatty acid derivatives in the detection results. This masks the true characteristic of Epiphyllum oxypetalum's VOCs being mainly terpenoid compounds in its natural state, making it impossible to accurately identify the source and release pattern of key aroma components.

[0004] Until the application of dynamic headspace collection-gas chromatography-mass spectrometry (DHC-GC-MS) technology, the spatiotemporal release characteristics of VOCs of Phalaenopsis amabilis were first determined: the total amount of VOCs released by Phalaenopsis amabilis began to significantly increase at about 21:00 at night, and peaked at about 23:00, of which terpenoids accounted for as high as 54.5%, being the core component of aroma; meanwhile, 5 different VOCs that played a key role in the aroma of Phalaenopsis amabilis were identified, namely, geraniol (accounting for 76.13% of the total amount of VOCs, presenting a sweet rose aroma), methyl salicylate (wintergreen odor), citral (lemon aroma), benzyl alcohol (light fruity fragrance), and benzyl valerate (mixed flower and fruit fragrance), and these key components were mainly derived from petals (49.4% of the total amount of VOCs released by petals). However, the study only stayed at the level of component analysis, and failed to convert this scientific discovery into industrialized extraction preparation and application technology, resulting in a serious disconnection between the existing Phalaenopsis amabilis extraction preparation process and the natural law of aroma components.

[0005] In terms of Phalaenopsis amabilis extraction preparation technology, there are three major problems in the existing scheme: first, the raw material collection lacks scientific basis. The harvesting time and site are not determined based on the spatiotemporal law of VOCs release, and Phalaenopsis amabilis in the daytime or non-blooming stage is often randomly selected, and organs such as stamens and pistils that are not the main source of aroma are also collected, resulting in extremely low content of key components such as geraniol and methyl salicylate in the extract; meanwhile, the influence of the harvesting environment (such as high moisture content of flowers due to harvesting in rainy days or high humidity nights) is ignored, further reducing the quality of the raw material. Second, the extraction process is unreasonable. The existing method fails to consider the characteristics of VOCs of Phalaenopsis amabilis (mainly terpenoids) that are volatile and easily damaged at high temperatures, or uses excessively high extraction temperature (such as more than 40℃) to cause loss of aroma components, or selects inappropriate solvents (such as organic solvents containing foreign odors) to introduce foreign odors, and fails to match and optimize the extraction parameters (such as supercritical CO2 extraction pressure, temperature, and flow rate) for different pretreated raw materials (fresh blocks, freeze-dried powders, and filaments), resulting in low purity and poor aroma reduction of the extract, and failing to preserve the complete aroma levels of Phalaenopsis amabilis, i.e., “fresh and clear at the beginning, rich and sweet in the middle, and light and lingering at the end”. Third, the resource utilization rate is low. During the preparation of traditional Phalaenopsis amabilis dried flowers, a large amount of flavor substances (containing key VOCs) lost with the evaporation of water are directly discarded without recycling, causing waste of resources and missing the way to obtain high-value fragrance bases.

[0006] In summary, there is an urgent need in the current technical field to develop new applications of Phalaenopsis amabilis, to promote the extension of Phalaenopsis amabilis from ornamental and medicinal use to high-value fragrance bases and other industries, and to fill the technical gap of Phalaenopsis amabilis in the utilization of spices. SUMMARY

[0007] The purpose of the present application is to solve the above technical problems, and to provide an application of Phalaenopsis amabilis extract in the preparation of perfume, which has high aroma reduction degree and good product stability.

[0008] The present application is realized by the following technical solutions:

[0009] The application of the orchid extract in the preparation of perfume, the orchid extract is composed of orchid extract ①, orchid extract ②, orchid extract ③ and orchid extract ④,

[0010] The orchid extract ① is obtained by low-temperature ethanol extraction method, and the content of geraniol in the orchid extract ① is greater than or equal to 62.5%, the content of benzyl alcohol is greater than or equal to 9.8%, and the content of linalool is greater than or equal to 1.06%;

[0011] The orchid extract ② is obtained by supercritical CO2 extraction method, and the content of geraniol in the orchid extract ② is greater than or equal to 75%, the content of methyl salicylate is greater than or equal to 4.3%, the content of citral is greater than or equal to 2.23%, and the content of benzyl alcohol is greater than or equal to 10%;

[0012] The orchid extract ③ is obtained by oil extraction method, and the content of geraniol in the orchid extract ③ is greater than or equal to 66.5%, the content of rosmarine is greater than or equal to 6.35%, the content of farnesene is greater than or equal to 2.3%, and the content of pinene is greater than or equal to 1.25%;

[0013] The orchid extract ④ is obtained by steam distillation method, and the content of geraniol in the orchid extract ④ is greater than or equal to 58%, the content of methyl salicylate is greater than or equal to 4.3%, the content of citral is greater than or equal to 2.23%, the content of benzyl alcohol is greater than or equal to 10%, and the content of benzyl salicylate is greater than or equal to 1.22%;

[0014] The mass ratio of the orchid extract ①, the orchid extract ②, the orchid extract ③ and the orchid extract ④ is 10-25:70-85:2-8:0.5-3;

[0015] The orchid extract is mixed with the auxiliary materials acceptable for the preparation of perfume, the auxiliary materials include at least one of solvent, stabilizer and fragrance enhancer, and the mixture is uniformly mixed to prepare the perfume.

[0016] Further, the orchid extract ① includes volatile aroma components and non-volatile components, wherein the volatile aroma components include geraniol, benzyl alcohol, linalool, benzaldehyde and its glycosides; the non-volatile components include flavonoids, phenolic acids, polysaccharides, amino acids, pigments and trace waxes.

[0017] Further, the orchid extract ② includes terpenes, esters, alcohols, aldehyde ketone volatile aroma compounds and fat-soluble substances;

[0018] The terpene substances include rosmarine, farnesene and caryophyllene; the ester substances include benzyl acetate, methyl salicylate, benzyl salicylate and benzyl benzoate; the alcohol substances include geraniol, linalool, benzyl alcohol and nerol; the aldehyde ketone substances include citral, nonanal and benzaldehyde.

[0019] Further, the orchid extract ③ comprises high fat-soluble compounds, the high fat-soluble compounds comprising sesquiterpenes, long-chain aliphatic hydrocarbons, wax esters, fat-soluble aroma precursors.

[0020] Further, the orchid extract ④ comprises water-soluble ingredients and partial volatile substances, the water-soluble ingredients comprising sugars, pectin, amino acids, water-soluble vitamins, glycosides; the volatile substances comprising geraniol, geranial, neral, benzaldehyde, melonal, methyl salicylate, citral, benzyl salicylate, benzyl benzoate, nerol.

[0021] Further, the method steps for obtaining the orchid extract ① by ethanol extraction are as follows:

[0022] a1, raw material harvesting: select orchids in full bloom, harvest the petals at 10 pm to 2 am; use a sterilized sharp tool to cut off the flower stem 1-2 cm from the plant, and place the petals in a clean soft container after harvesting, avoiding rainy days or high humidity nights;

[0023] b1, immediately transfer to the processing workshop after harvesting, remove diseased and damaged petals, and then store them in liquid nitrogen or dry ice; rinse the petals with deionized water and dry them, then cut them into 0.5±0.8 cm square pieces;

[0024] c1, mix according to the mass / volume ratio of orchid: food-grade anhydrous ethanol 1:6-1:9, put into a brown glass bottle with a sealing cap, and extract at 4-8℃ for 24-28h;

[0025] d1, filter the residue with a 300 mesh sieve after extraction, filter the filtrate through a 0.22-0.28 μm microporous filter membrane after dilution, and collect the filtrate, which is the orchid extract ①.

[0026] Further, the method steps for obtaining the orchid extract ② by ethanol extraction are as follows:

[0027] a2, raw material harvesting: select orchids in full bloom, harvest the petals at 10 pm to 2 am; use a sterilized sharp tool to cut off the flower stem 1-2 cm from the plant, and place the petals in a clean soft container after harvesting, avoiding rainy days or high humidity nights;

[0028] b2, immediately transfer to the processing workshop after harvesting, remove diseased and damaged petals, and then store them in liquid nitrogen or dry ice; vacuum freeze-dry the petals at -50℃, and then crush them into 80 mesh powder;

[0029] c2, supercritical CO2 extraction is used for the orchid powder in step a2, and the extraction parameters are set as follows: extraction pressure 30-35 MPa, extraction temperature 35-39℃, CO2 flow rate 20-24 L / h, extraction time 2-2.5h;

[0030] d2, remove impurities by secondary separation method, the first separation parameters are pressure 10-13 MPa, temperature 40-43℃, the second separation parameters are pressure 5 MPa, temperature 30-34℃, collect the separated extract, which is Epiphyllum extract ②.

[0031] Further, the method steps for obtaining Epiphyllum extract ③ by ethanol extraction method are:

[0032] a3, raw material collection: select Epiphyllum in full bloom, collect the petal pieces at 10pm-2am; cut off the flower stem 1-2cm from the plant with sterilized sharp tools, and place the collected petal pieces in a clean soft container, avoiding rainy days or high humidity nights;

[0033] b3, immediately transfer to the processing workshop after collection, remove diseased and damaged petal pieces, and then store in liquid nitrogen or dry ice; wash and drain the petal pieces, and then tear them into fine filaments;

[0034] c3, mix the odorless liquid paraffin or glycerol as carrier with the petal pieces at a mass ratio of 1:4-5, and load into a sealed glass jar, and place in a cool and dark place at 10-15℃ for 7 days;

[0035] d3, filter the petal residues with a 200 mesh sieve after extraction, and collect the extract, which is Epiphyllum extract ③.

[0036] Further, the method steps for obtaining Epiphyllum extract ④ by ethanol extraction method are:

[0037] a4, raw material collection: select Epiphyllum in full bloom, collect the petal pieces at 10pm-2am; cut off the flower stem 1-2cm from the plant with sterilized sharp tools, and place the collected petal pieces in a clean soft container, avoiding rainy days or high humidity nights;

[0038] b4, immediately transfer to the processing workshop after collection, remove diseased and damaged petal pieces, and then store in liquid nitrogen or dry ice; wash and drain the petal pieces, and then tear them into fine filaments;

[0039] c4, air-dry the petal pieces at 35-42℃, connect a condensing device to the air outlet of the drying box, and start collecting the volatile flavoring substances after 1-2h of drying; every 30-35min, transfer the collected extract to a sterile container with a sterile pipette;

[0040] d4, filter the extract to remove impurities immediately after collection, and then filter through a 0.22-0.28μm microporous filter, and then concentrate under reduced pressure at 40-45℃ to obtain a concentrated solution, which is Epiphyllum extract ④.

[0041] Further, the sensory indicators of the Epiphyllum extract meet the following: clear and transparent appearance, no suspended matter and precipitate, colorless or slightly yellow color, unique Epiphyllum fragrance, and no odor.

[0042] Further, the Epiphyllum extract meets any one of the following:

[0043] a) adding 0.02-0.23 wt% vitamin E or rosemary extract, sealing, and storing at -18°C;

[0044] b) storing in a dark sealed glass bottle at <20°C in a cool and dry place.

[0045] Further, the preparation of the perfume includes the following steps:

[0046] (1) raw material preparation: preparing raw materials according to the ratio, the raw materials including Epiphyllum extract, auxiliary materials, the Epiphyllum extract accounting for 11.5-15.0% of the total mass of the raw materials, and the auxiliary materials including double-dealcohol, neral formate, orange flower extract, methyl salicylate, isoamyl acetate, geraniol, hexenal, heptanal, Epiphyllum extract, indole, phenethyl alcohol, gardenia flower extract, vanillin, musk ambrette, jasmine extract, peony main agent, and propylene glycol;

[0047] (2) mixing the perfume: first dissolving the Epiphyllum extract in the Epiphyllum extract in propylene glycol, the concentration of the propylene glycol being 1.5-2 wt%, to obtain a propylene glycol solution of the Epiphyllum extract; and then mixing the remaining Epiphyllum extract with the auxiliary materials according to the set ratio and stirring uniformly;

[0048] (3) dissolving in a solvent: adding the mixed perfume into double-dealcohol and stirring until completely dissolved;

[0049] (4) standing and aging: standing the dissolved mixture for 5-6 weeks;

[0050] (5) filtering: filtering the aged mixture through a microporous filter membrane to remove impurities;

[0051] (6) bottle storage: storing the filtered perfume in a perfume bottle and sealing.

[0052] Further, in step (2), the Epiphyllum extract, Epiphyllum extract, orange flower extract, gardenia flower extract, and jasmine extract are first mixed uniformly, and then neral formate, methyl salicylate, isoamyl acetate, geraniol, hexenal, heptanal, indole, phenethyl alcohol, vanillin, musk ambrette, peony main agent are added and stirred.

[0053] Further, in step (3), the stirring speed is controlled to be 50-80 r / min, and the stirring time is 30-60 min, to ensure that the perfume is completely dissolved in the double-dealcohol.

[0054] Further, the standing aging environment in step (4) is a light-proof environment with a temperature of 20-25 DEG C and a relative humidity of 40-60%.

[0055] Further, in step (5), the pore size of the microporous filter membrane is 0.22-0.28 mu m.

[0056] Compared with the prior art, the present application has the following advantages and beneficial effects:

[0057] First, in the present application, the prior art obtains vanilla extract by four methods, i.e., ethanol low-temperature extraction method, supercritical CO2 extraction method, oil extraction method and water vapor distillation method. However, when the four extracts are used alone to prepare vanilla perfume essence, there are obvious defects. Specifically, the vanilla extract obtained by the ethanol low-temperature extraction method has poor volatility and complex odor components, and cannot highlight the unique aroma of vanilla; the vanilla extract obtained by the supercritical CO2 extraction method is rich in high-concentration volatile aroma molecules, but has the problems of "flatness" of the aroma and over-concentration of the aroma which is easy to cause nasal irritation; the vanilla extract obtained by the oil extraction method has soft aroma, but deviates from the original flavor of vanilla because the sweet tone is too prominent; the vanilla extract prepared by the water vapor distillation method has weak aroma intensity and obvious "cooking flavor", and cannot meet the quality requirements of high-end perfume for aroma quality. Therefore, the present application innovatively compounding the four extracts according to a specific perfuming ratio, realizing odor "tailoring" by the synergistic effect of the components, complementing the advantages of different extracts to cover their respective defects, and further constructing a better aroma system to ultimately prepare high-end vanilla perfume with stable quality, and the aroma of which can fully present the natural characteristics of "fresh and clear at the beginning - rich sweet aroma at the middle - delicate aftertaste at the end".

[0058] Second, in the present application, the ethanol low-temperature extraction method, the supercritical CO2 extraction method, the oil extraction method and the water vapor distillation method are used to obtain four kinds of vanilla extracts, i.e., vanilla extract ①, vanilla extract ②, vanilla extract ③ and vanilla extract ④. Then, the dosage ratio of the vanilla extract ①, the vanilla extract ②, the vanilla extract ③ and the vanilla extract ④ in the preparation of vanilla perfume is determined by fully considering the aroma intensity, volatile level, solubility compatibility and sensory synergy of vanilla, etc. The dosage ratio of the four extracts determines the structure, stability and final aesthetic sense of the perfume.

[0059] Third, in the present application, the compatibility problem between different extracts is solved. The base solvent of the perfume is ethanol, which has excellent compatibility with the vanilla extract ①, the vanilla extract ② and the vanilla extract ③. However, the vanilla extract ④ is water-soluble, and direct addition into the ethanol-based perfume will cause turbidity, flocculation and even stratification, which will destroy the clear appearance and stability of the perfume. Therefore, it is necessary to pre-mix it with a cosolvent (such as a "water-ethanol" cosolvent system) to ensure that it is completely dissolved in the final product.

[0060] Fourthly, in the present application, the method can obtain perfume with fresh and clear at the beginning, rich and sweet in the middle, and light and lingering at the end, and stable quality. Epiphyllum extract ② is rich in a large number of top note / leading note ingredients (such as monoterpene, low molecular weight aldehyde, etc.), which volatilize the fastest and are smelled first; Epiphyllum extract ① contains more body note / middle note (such as alcohol, ester) and base note / trailing note (such as sesquiterpene, wax), which volatilize slowly and are responsible for the core characteristics and long-lasting of the perfume; Epiphyllum extract ④ mainly provides a "water feeling" and a slight top note support.

[0061] Fifthly, in the present application, the proportion of the compound of Epiphyllum extract (compound of Epiphyllum extract ①, Epiphyllum extract ②, Epiphyllum extract ③ and Epiphyllum extract ④) is precisely controlled to realize the directional "cutting" of the overall odor, and the synergistic effect between different ingredients is used to optimize the aroma performance, and the advantages of each raw material are used to make up for potential defects. The specific control logic is as follows: Epiphyllum extract ③ has a soft sweet quality, which can effectively neutralize the sharp aroma that may exist in Epiphyllum extract ②, making the overall fragrance more round and coordinated; Epiphyllum extract ① contains a wide range of aroma ingredients, which can provide a rich aroma background for the perfume, significantly improving the thickness and complexity of the fragrance; For the "boiled taste" that may exist in Epiphyllum extract ④, Epiphyllum extract ② with rich and fresh floral fragrance can be used to cover up and modify it, so as to effectively avoid the defects of the aroma and improve the quality of the overall fragrance. BRIEF DESCRIPTION OF DRAWINGS

[0062] Figure 1 is the anatomical diagram of Epiphyllum.

[0063] Figure 2 is a statistical chart of the change of Epiphyllum flower fragrance over time during the flowering process. DETAILED DESCRIPTION

[0064] The present application will be further described in conjunction with the examples below, but the embodiments of the present application are not limited thereto.

[0065] Example 1

[0066] In order to facilitate the public to understand the present application, the present embodiment provides a perfume preparation scheme taking Epiphyllum extract as the core fragrance raw material, which fully utilizes the unique natural fragrance of Epiphyllum, combines scientific extraction process and precise formula ratio, and prepares high-end perfume with fresh and clear at the beginning, rich and sweet in the middle, and light and lingering at the end, and stable quality, while realizing efficient utilization of Epiphyllum resources and improving its economic value.

[0067] 1. Epiphyllum raw material collection and pretreatment.

[0068] 1.1, Collection site.

[0069] Reference Figure 1 The perianth of the flower of the plant of the genus Plumeria during the flowering stage is selected as the raw material. The perianth contains petals and sepals and is the main active ingredient (such as polysaccharides, flavonoids) and aromatic substance of Plumeria, and can provide a rich and pure Plumeria fragrance base for perfume.

[0070] 1.2, Collection time.

[0071] It is found through investigation and research that the release of the Plumeria flower fragrance has significant time effectiveness and dynamic change characteristics. The flower fragrance is mainly released during the opening of the flower, and the release rule of the flower fragrance components presents a high degree of dynamics. Specifically, from about 8 pm when the flower begins to open to 12 am when the flower is fully open, the release amount of the Plumeria flower fragrance volatile realizes a large increase of 200 times. The increase process mainly starts at 10 pm, and reaches a release peak at 1 am. After 2 am, the release amount gradually decreases. Figure 2 In addition, among the Plumeria flower fragrance components, the core component accounting for 72.54% of the total release amount, geraniol, has a release rule completely consistent with the overall volatile release rule. The release of the other two main components presents a unique time characteristic. The two release peaks of methyl salicylate are at 10-11 pm and 6-7 am the next day, respectively. The release peak of benzyl alcohol is concentrated in the early morning of 2-4 am.

[0072] According to the release rule of the Plumeria aromatic substances, collection is performed at 10 pm to 2 am. At this stage, the Plumeria is full and has bright color, and the content of the active ingredient is high. The release amount of the main fragrance component geraniol, which accounts for about 72.54% of the total release amount, reaches a peak, and the core fragrance substance of Plumeria can be obtained to the maximum extent. At the same time, rain and high air humidity at night are avoided to prevent the flower from having too high water content and affecting subsequent processing.

[0073] 1.3, Collection method.

[0074] A sharp scissors that has been sterilized is used. The collector holds the Plumeria flower base with one hand and cuts the flower stem about 1-2 cm away from the plant with the scissors with the other hand to ensure that the flower stem is complete and the main body of the flower is not damaged. The cut Plumeria is placed in a clean and soft shallow basket in a single layer to avoid deformation or damage of the flowers caused by mutual extrusion and reduce the risk of microbial infection.

[0075] 2, Storage after collection.

[0076] After the collection is completed, the Plumeria is immediately transferred to the processing workshop, the flowers with pests and diseases are selected out, and the remaining high-quality flowers are quickly placed in liquid nitrogen for storage (if the production conditions of the enterprise are limited, dry ice can also be used for storage) to maximize the locking of the original components of the Plumeria flower fragrance and ensure the excellent quality of the subsequent extraction raw material.

[0077] 3. Preparation of Epiphyllum extract.

[0078] In this example, four different processes were used to prepare Epiphyllum extract, namely Epiphyllum extract ① (ethanol low-temperature extraction method), Epiphyllum extract ② (supercritical CO2 extraction method), Epiphyllum extract ③ (oil extraction method), and Epiphyllum extract ④ (steam distillation method).

[0079] 3.1. Preparation of Epiphyllum extract ① by ethanol low-temperature extraction method, with the following steps:

[0080] a1. Raw material pretreatment: Take out the fresh Epiphyllum stored in liquid nitrogen, remove the non-scented parts such as flower stems, and only keep the petal pieces. Rinse the petal pieces quickly with deionized water, then absorb the surface moisture with sterile gauze (to avoid diluting the subsequent extraction solvent), cut them into small pieces of 0.5 cm square, and reserve them for use.

[0081] b1. Select food-grade anhydrous ethanol (purity ≥ 95%, no odor) as the extraction solvent, and mix the petal pieces with ethanol at a mass-volume ratio of 1:6. Put the mixture into a brown glass bottle with a sealed lid (brown bottle can block light and prevent photooxidation from damaging the aroma substances).

[0082] c1. Low-temperature extraction: Place the brown glass bottle containing the mixture in a refrigerated environment at 4-8°C and let it stand for 24 hours. The low-temperature environment can delay the volatilization of aroma components, and the 24-hour extraction time can ensure that the aroma components are fully dissolved without the need for stirring to avoid mechanical changes to the aroma molecular structure.

[0083] d1. Separation and purification: After extraction, filter the mixture with a 300-mesh sieve to remove the petal residues and collect the filtrate. Transfer the filtrate to a low-temperature rotary evaporator, set the temperature to 35°C (lower than the boiling point of ethanol, 78°C, to avoid high-temperature damage to the aroma and loss of aroma components due to boiling), and set the vacuum degree to 0.09 MPa. Slowly evaporate the ethanol until the filtrate becomes a viscous essential oil. Then dilute the viscous extract with a small amount of deionized water, filter it through a 0.22 μm microporous filter to remove trace impurities, and obtain clear Epiphyllum extract ①.

[0084] Storage: Immediately add 0.02% vitamin E (as a natural antioxidant to prevent oxidation and deterioration of aroma components) to the Epiphyllum extract ①, seal it, and store it at -18°C for future use.

[0085] The extract of Epiphyllum oxypetalum is detected and analyzed by gas chromatography-mass spectrometry and liquid chromatography. The extract of Epiphyllum oxypetalum includes volatile aroma components and non-volatile components. The volatile aroma components include geraniol, benzyl alcohol, linalool, benzaldehyde and its glycosides. The non-volatile components include flavonoids (such as glycosides of kaempferol and quercetin), phenolic acid, polysaccharide, amino acid, pigment and trace wax. The content of geraniol in the extract of Epiphyllum oxypetalum is 62.5%, the content of benzyl alcohol is 9.9%, and the content of linalool is 1.1%.

[0086] 3.2, the extract of Epiphyllum oxypetalum is prepared by supercritical CO2 extraction method, and the steps are as follows:

[0087] a2, raw material pretreatment: the fresh Epiphyllum oxypetalum stored in liquid nitrogen is freeze-dried, and the freeze-drying temperature is set to-50 DEG C. The drying is completed in a vacuum environment to avoid the destruction of aroma substances by high-temperature drying. The dried Epiphyllum oxypetalum is crushed into 80 mesh powder to increase the contact area with the extractant and improve the extraction efficiency.

[0088] b2, supercritical extraction operation: the Epiphyllum oxypetalum powder is put into the supercritical extraction equipment, and the extraction parameters are set as follows: extraction pressure 30 MPa (the solubility of supercritical CO2 to aroma components is best under this pressure), extraction temperature 35 DEG C (low temperature can protect aroma components from being destroyed), CO2 flow rate 20 L / h, and extraction time 2 h to ensure that all volatile aroma components are fully dissolved.

[0089] c2, separation and collection: the extract product is treated by two-stage separation method. The first-stage separation is set to a pressure of 10 MPa and a temperature of 40 DEG C, mainly for removing impurities; the second-stage separation is set to a pressure of 5 MPa and a temperature of 30 DEG C, and the light yellow transparent and pure Epiphyllum oxypetalum extract 2 is collected.

[0090] Storage: the Epiphyllum oxypetalum extract 2 is packed into dark and sealed glass bottles and stored in a cool and dry place with a temperature of less than 20 DEG C for standby.

[0091] The extract of Epiphyllum oxypetalum is detected and analyzed by gas chromatography-mass spectrometry and liquid chromatography. The main components of the extract of Epiphyllum oxypetalum are volatile aroma compounds and fat-soluble substances. The terpene substances include linalool, farnesene and caryophyllene; the ester substances include benzyl acetate, methyl salicylate, benzyl salicylate and benzyl benzoate; the alcohol substances include geraniol, linalool, benzyl alcohol and nerol; the aldehyde and ketone substances include citral, nonanal and benzaldehyde. The content of geraniol in the extract of Epiphyllum oxypetalum is 75.1%, the content of methyl salicylate is 4.4%, the content of citral is 2.3%, and the content of benzyl alcohol is 10.3%.

[0092] 3.3, the extract of Epiphyllum oxypetalum is prepared by oil extraction method, and the steps are as follows:

[0093] a3, raw material preparation: take out the fresh flower preserved in liquid nitrogen, remove the flower stem, wash and drain the petals, tear into fine filaments, increase the contact area with the extraction solvent, and promote the dissolution of aroma components.

[0094] b3, select odorless liquid paraffin as the extraction carrier (glycerol can also be selected according to demand), mix the fresh flower petal filaments and liquid paraffin at a mass ratio of 1:4, and load into a sealed glass jar.

[0095] c3, low-temperature extraction: place the sealed glass jar in a cool place (avoid direct sunlight) at 10-15°C, and stand for 7 days. During the extraction period, gently shake the glass jar once a day to promote the dissolution of aroma components, while avoiding vigorous shaking to damage the structure of the aroma substances.

[0096] d3, filtration separation: after 7 days of extraction, filter the mixture with a 200-mesh sieve to remove the petal residue, and collect the yulan magnolia extract ③.

[0097] Storage: store the yulan magnolia extract ③ in a dark, sealed glass bottle in a cool, dry place at a temperature of <20°C for future use.

[0098] The yulan magnolia extract ③ was detected and analyzed by gas chromatography-mass spectrometry and liquid chromatography, and the main components of the yulan magnolia extract ③ were found to be high-fat-soluble compounds. Among them, high-boiling terpenes: sesquiterpenes (such as farnesene, myrrhene); long-chain alkanes, wax esters, and fat-soluble aroma precursors. And it is measured that in the yulan magnolia extract ③, geraniol: 66.5%, basilene: 6.7%, farnesene: 2.4%, pinene: 1.3%.

[0099] 3.4, yulan magnolia extract ④ was prepared by steam distillation method, the specific steps are as follows:

[0100] a4, drying and condensation collection: place the fresh yulan magnolia preserved in liquid nitrogen into a forced air drying oven, set the drying temperature to 35°C. Connect the condensation device to the exhaust port of the drying oven, and start collecting the extract when the yulan magnolia is dried for about 1 hour. The low-temperature drying environment at 35°C can promote slow water loss in cells, avoid cell rupture due to high temperature, and prevent loss of components. After the water in the cells seeps out of the cell membrane to the surface of the flower, it drips into the water pan and enters the condensation device.

[0101] b4, collection frequency and operation: check the collection container every 30 minutes, use a sterile pipette to transfer the collected extract to a sterile container to prevent contamination or evaporation due to long-term exposure to the oven.

[0102] C4, filtration and concentration: the collected extract is immediately subjected to preliminary filtration to remove impurities, and then to fine filtration through a 0.22 μm microporous filter to remove microorganisms. Subsequently, the filtered filtrate is placed in a reduced-pressure concentration device and subjected to low-temperature concentration below 40°C to increase the concentration of effective components of the extract, thereby obtaining the orchid extract.

[0103] Storage: the orchid extract IV is filled into a dark, sealed glass bottle and stored in a cool, dry place at a temperature <20°C for standby use.

[0104] The orchid extract IV is detected and analyzed by gas chromatography-mass spectrometry and liquid chromatography. The main components of the orchid extract IV are water-soluble components and part of volatile components. The water-soluble components are sugars (fructose, glucose), pectin, amino acids (aspartic acid, glutamic acid), water-soluble vitamins, glycosides, and "cooked aroma" molecules such as furans and pyrazines generated by thermal effects. The volatile substances include geraniol, geranial, neral, benzaldehyde, melonal, methyl salicylate, citral, benzyl salicylate, benzyl benzoate, and nerol. The measured contents of geraniol, methyl salicylate, citral, benzyl alcohol, and benzyl salicylate in the orchid extract IV are 58.5%, 4.3%, 2.4%, 10.5%, and 1.3%, respectively.

[0105] 4. Perfume preparation.

[0106] 4.1. The formula of the perfume "Luna's Beauty" (for preparing 1000 g of the perfume) is shown in Table 1.

[0107] Table 1:

[0108]

[0109] 4.2. Modulation steps.

[0110] (1) Preparation of raw materials: the raw materials are prepared according to Table 1 above.

[0111] (2) Mixing of spices: according to the above formula, the orchid extract IV in the orchid extract is first dissolved in 2 wt% propylene glycol to obtain an orchid extract IV propylene glycol solution; then the remaining orchid extract, citral, orange extract, methyl salicylate, isoamyl acetate, geraniol, hexenal, heptanal, orchid extract, indole, phenethyl alcohol, gardenia extract, vanillin, musk ambrette, jasmine extract, and peony extract are sequentially added to a sterile mixing container, and a sterile stirrer is used to stir at a speed of 50 r / min for 15 min to ensure uniform mixing of all spice components.

[0112] (3) Dissolve in solvent: slowly add the mixed perfume into the sterile blending tank containing double-dealcoholized alcohol, turn on the stirring function of the blending tank, and stir at a speed of 80 r / min for 30 min until the perfume is completely dissolved in the alcohol to form a uniform mixture.

[0113] (4) Standing and aging: seal the blended mixture and place it in an environment with a temperature of 20-25°C, away from light, and good ventilation for standing and aging for 5-6 weeks. During the aging process, the aroma components are fully integrated and balanced, and part of the irritating odor can be removed to improve the roundness and stability of the perfume aroma.

[0114] (5) Filtration: after the aging is completed, the mixture is filtered through a 0.22 μm microporous filter to remove trace impurities and insoluble particles in the mixture, ensuring that the perfume is clear and transparent without suspended matter and precipitate.

[0115] (6) Bottling and storage: the filtered perfume is filled into pre-sterilized dark perfume bottles (dark bottles can block light and prevent photo-oxidation of aroma components) using sterile filling equipment, sealed, labeled, and stored in a cool and dry place.

[0116] Quality detection of perfume.

[0117] (I) Sensory index detection.

[0118] Odor: a review team consisting of 20 professional perfumers evaluates using the sniffing method, and the sensory comprehensive score is the sum of the scores of appearance, color, and odor. The review team's scoring standards are shown in Table 2.

[0119] Table 2

[0120]

[0121] The test results are shown in Table 3.

[0122] (II) Physicochemical index detection.

[0123] pH value: the pH value of the perfume is detected using a precision pH meter, and the detection result should be within the range of 5.5-7.0 (close to neutral), meeting the requirements of skin friendliness.

[0124] Soluble solids content: the refractometer is used for detection, and the soluble solids content of the perfume should be ≤0.5%, ensuring low sugar and low impurities to avoid affecting the stability and use of the perfume.

[0125] Mercury content: Chapter 4, Section 1.2 of the "Cosmetic Safety Technical Specifications" (2015 edition) uses the hydride atomic fluorescence spectrophotometric method, with a limit of ≤1.

[0126] Lead content: Chapter 4, 1.3 of the Cosmetic Safety Technical Specifications (2015 Edition), Flame Atomic Absorption Spectrophotometry, limit ≤10.

[0127] Arsenic content: Chapter 4, 1.4 of the Cosmetic Safety Technical Specifications (2015 Edition), Hydride Atomic Fluorescence Spectrophotometry, limit ≤2.

[0128] Cadmium content: Chapter 4, 1.5 of the Cosmetic Safety Technical Specifications (2015 Edition), Flame Atomic Absorption Spectrophotometry, limit ≤5.

[0129] Methanol content: Chapter 4, 2.22 of the Cosmetic Safety Technical Specifications (2015 Edition), Gas Chromatography, limit ≤2000.

[0130] The test results are shown in Table 3.

[0131] Table 3

[0132]

[0133] After testing, the perfumes prepared by the method of the present embodiment are all free of odor deterioration, no "old smell" is produced after oxidation, the appearance and color are good, the pH value is close to neutral, and the microbial indicators all meet the requirements of the Cosmetic Safety Technical Specifications (2015 Edition) and the Perfume, Eau de Cologne (QB / T 1858-2004) standards, proving that the perfume stability is good and can meet the market circulation and use requirements.

[0134] Example 2

[0135] The difference between the present example and Example 1 is only that the ratio of the four components of Epiphyllum oxypetalum Willd extract ①, Epiphyllum oxypetalum Willd extract ②, Epiphyllum oxypetalum Willd extract ③ and Epiphyllum oxypetalum Willd extract ④ in the raw material is different, and the raw material ratio is shown in Table 4.

[0136] Table 4

[0137]

[0138] The same method steps as in Example 1 were used to prepare the perfume.

[0139] The same detection method as in Example 1 was used to detect the quality of the perfume, and the test results are shown in Table 5.

[0140] Table 5

[0141]

[0142] The perfume prepared by the method of the embodiment is tested, and the perfume has no change in fragrance, no "old smell" after oxidation, good appearance and color, pH value close to neutral, and microbial indicators meeting the requirements of the Cosmetic Safety Technical Specifications (2015 edition) and the Perfume and Eau de Cologne (QB / T 1858-2004) standards, proving that the perfume has good stability and can meet the market circulation and use requirements.

[0143] Example 3

[0144] The embodiment is compared with Example 1, and the difference is only that the proportions of the four components of the raw material - Epiphyllum extract ①, Epiphyllum extract ②, Epiphyllum extract ③ and Epiphyllum extract ④ are different, and the raw material proportions are shown in Table 6.

[0145] Table 6

[0146]

[0147] The perfume is prepared by the same method steps as Example 1.

[0148] The quality of the perfume is detected by the same detection method as Example 1, and the detection results are shown in Table 7.

[0149] Table 7

[0150]

[0151] The perfume prepared by the method of the embodiment is tested, and the perfume has no change in fragrance, no "old smell" after oxidation, good appearance and color, pH value close to neutral, and microbial indicators meeting the requirements of the Cosmetic Safety Technical Specifications (2015 edition) and the Perfume and Eau de Cologne (QB / T 1858-2004) standards, proving that the perfume has good stability and can meet the market circulation and use requirements.

[0152] Comparative Example 1

[0153] The comparative example is compared with Example 1, and the difference is only that the Epiphyllum extract in the raw material is all Epiphyllum extract ①, and the amount is 140 g, and the other raw materials and their amounts are the same as Example 1.

[0154] The steps and methods for preparing the perfume are the same as Example 1, and the perfume is obtained.

[0155] Similarly, the perfume of the comparative example is detected by the same quality detection method as Example 1, and the detection results are shown in Table 8.

[0156] Comparative Example 2

[0157] The comparative example is compared with Example 1, the only difference is that the raw material of Epiphyllum extract is all Epiphyllum extract 2, the amount is 140 g, and the rest of the raw materials and their amounts are the same as Example 1.

[0158] The steps of preparing the perfume are the same as Example 1, and the perfume is obtained.

[0159] Similarly, the perfume of the comparative example is detected by the same quality detection method as Example 1, and the detection results are shown in Table 8.

[0160] Comparative Example 3

[0161] The comparative example is compared with Example 1, the only difference is that the raw material of Epiphyllum extract is all Epiphyllum extract 3, the amount is 140 g, and the rest of the raw materials and their amounts are the same as Example 1.

[0162] The steps of preparing the perfume are the same as Example 1, and the perfume is obtained.

[0163] Similarly, the perfume of the comparative example is detected by the same quality detection method as Example 1, and the detection results are shown in Table 8.

[0164] Comparative Example 4

[0165] The comparative example is compared with Example 1, the only difference is that the raw material of Epiphyllum extract is all Epiphyllum extract 4, the amount is 140 g, and the rest of the raw materials and their amounts are the same as Example 1.

[0166] The steps of preparing the perfume are the same as Example 1, and the perfume is obtained.

[0167] Similarly, the perfume of the comparative example is detected by the same quality detection method as Example 1, and the detection results are shown in Table 8.

[0168] Comparative Example 5

[0169] The comparative example is compared with Example 1, the only difference is that the raw material of Epiphyllum extract is 70 g of Epiphyllum extract 1 + 70 g of Epiphyllum extract 2, and the rest of the raw materials and their amounts are the same as Example 1.

[0170] The steps of preparing the perfume are the same as Example 1, and the perfume is obtained.

[0171] Similarly, the perfume of the comparative example is detected by the same quality detection method as Example 1, and the detection results are shown in Table 8.

[0172] Comparative Example 6

[0173] The comparative example is compared with Example 1, the only difference is that the raw material of Epiphyllum extract is 70 g of Epiphyllum extract 2 + 70 g of Epiphyllum extract 4, and the rest of the raw materials and their amounts are the same as Example 1.

[0174] The perfume was prepared by the same method as in Example 1.

[0175] Similarly, the perfume of the present comparative example was detected by the same quality detection method as in Example 1, and the detection results are shown in Table 8.

[0176] Table 8

[0177]

[0178] From the comparison of the perfumes prepared from Example 1 and Comparative Examples 1-4, it can be seen that:

[0179] The perfume prepared by using only the extract of Cattleya × hybrida ① as the main raw material has rich and complex fragrance, with a slight "dull" or "solvent bottom note". The levels are rich, and various tonalities such as flower, fruit and green can be felt, but the topmost and lightest fragrance may be lost, and the diffusion of the fragrance is moderate.

[0180] The perfume prepared by using only the extract of Cattleya × hybrida ② as the main raw material has the closest fragrance to natural Cattleya × hybrida, with high purity, fullness, freshness and rich levels. From the light "top note" to the thick "body note", it is well preserved. The fragrance intensity is high, the diffusion is strong, and there is no solvent odor, and the quality is the highest. However, if the concentration is too high, it will completely cover the delicate fragrance of other extracts, leading to "flat" fragrance, only a nose-piercing top note, and lack of middle and late notes.

[0181] The perfume prepared by using only the extract of Cattleya × hybrida ③ as the main raw material has soft, sweet and lasting fragrance, but the freshness and level are slightly weak. The fragrance is more inclined to the warm and powdery "base note" tonality (close to the fragrance of Cattleya × hybrida after direct drying), rather than the explosive fragrance of fresh flowers. The diffusion is weak, but the fragrance lasts for a long time.

[0182] The perfume prepared by using only the extract of Cattleya × hybrida ④ as the main raw material has weak fragrance, with a "boiled" taste, "cooked" taste or light taste similar to "vegetable juice", rather than strong flower fragrance. The fragrance is more focused on the "taste" brought by water-soluble ingredients.

[0183] From Comparative Examples 5 and 6, it can be seen that the perfumes prepared by using the extract of Cattleya × hybrida ② with the extract of Cattleya × hybrida ① and the extract of Cattleya × hybrida ④ are better than the perfume prepared by using only the extract of Cattleya × hybrida ②, neutralizing the nose-piercing top note, but compared with Examples 1-3, they still lack the freshness, long-lasting effect and rich levels of the fragrance.

[0184] In embodiments 1-3, the vanilla essences prepared by compounding multiple vanilla extracts focus more on the freshness, long-lasting fragrance effect and rich layers of the fragrance, and ultimately build a harmonious and stable core vanilla fragrance with distinct layers and elegant fragrance evolution over time. It should be noted that each vanilla extract is not a single compound, but a complex mixture system composed of multiple components with different chemical properties. Based on this characteristic, by screening and combining different extracts, the advantages of each extract can be fully utilized to achieve synergistic compounding and further optimize the overall fragrance performance of the essence. At the same time, the compounding ratio of vanilla extract ①, vanilla extract ②, vanilla extract ③ and vanilla extract ④ can be flexibly adjusted according to the actual application scenario or market demand, so as to meet the diversified fragrance customization needs.

[0185] The inventors of the present application have found through extensive experiments that:

[0186] I. The intensity of the fragrance of the four extracts is quite different.

[0187] The intensity of the fragrance of the four extracts vanilla extract ①, vanilla extract ②, vanilla extract ③ and vanilla extract ④ is quite different: the fragrance intensity of vanilla extract ② is usually the highest because it is rich in high concentrations of volatile fragrance molecules. However, if the proportion is too high, it will completely mask the delicate fragrance of other extracts, resulting in "flat" fragrance, only a nose-piercing top note, lacking middle and base notes; while the fragrance of vanilla extract ④ is the weakest, and if the proportion is too low, the "wateriness" and "freshness" it brings will completely disappear; if the proportion is too high, it may introduce an unpleasant "steaming" flavor.

[0188] II. The volatility and fragrance layers of the four extracts are different.

[0189] Vanilla extract ② is rich in a large number of top / middle notes (such as monoterpenes, low molecular weight aldehydes, etc.), which volatilize the fastest and are noticed first; vanilla extract ① contains more body / middle notes (such as alcohols, esters) and base / ending notes (such as sesquiterpenes, waxes), which volatilize slowly and are responsible for the core characteristics and long-lasting fragrance of the essence; vanilla extract ④ mainly provides a "wateriness" and a slight top note support.

[0190] III. Solubility and compatibility problems of the four extracts.

[0191] This is also the most critical technical bottleneck. The base solvent of the essence is ethanol, which has excellent compatibility with vanilla extract ①, vanilla extract ② and vanilla extract ③, while vanilla extract ④ is water-soluble, and direct addition to the ethanol-based essence will cause turbidity, flocculation and even layering, destroying the clear appearance and stability of the essence. It needs to be pre-mixed with a co-solvent (such as a "water-ethanol" co-solvent system) to ensure that it is completely dissolved in the final product.

[0192] Four, sensory synergy and masking.

[0193] Orchid extract ③ has a soft sweet aroma, which can round the too sharp aroma in orchid extract ②, and the broad-spectrum components in orchid extract ① can provide a rich background, increasing the thickness and complexity of the aroma. The "boiled taste" of orchid extract ④ needs to be masked and modified by the strong fresh flower aroma in orchid extract ②. By "tailoring" the odor through the ratio of ingredients, the synergistic effect between ingredients is used to create a better effect, and the advantages are used to mask the disadvantages.

[0194] In this embodiment, through scientific orchid raw material collection and pretreatment process, combined with four different orchid extract preparation methods, the natural aroma components of orchid are fully retained; then through precise perfume formula ratio and standardized modulation process, high-quality orchid perfume is successfully prepared. The perfume not only has the unique aroma levels of orchid, but also combines the elegant aroma of various auxiliary spices, and all quality indicators meet the requirements, and the stability is good. At the same time, in this embodiment, the flavor substances (orchid extract ④ prepared by condensation collection method) that may be discharged during the preparation of dry flowers are used for perfume modulation, realizing efficient utilization of orchid resources, improving its economic value, conforming to the green and sustainable development concept, and having good industrial application prospect and market competitiveness.

[0195] The above is only a preferred embodiment of the present application, and does not limit the present application in any form. Any simple modification or equivalent change made according to the technical essence of the present application to the above embodiment falls within the protection scope of the present application.

Claims

1. The application of an extract of Epiphyllum oxypetalum in the preparation of perfume, characterized in that: The epiphyllum extract is composed of epiphyllum extract ①, epiphyllum extract ②, epiphyllum extract ③, and epiphyllum extract ④. The Epiphyllum extract ① is an extract obtained by low-temperature ethanol extraction, and contains geraniol ≥ 62.5%, benzyl alcohol ≥ 9.8%, and linalool ≥ 1.06% in Epiphyllum extract ①. The Epiphyllum extract ② is an extract obtained by supercritical CO2 extraction. The Epiphyllum extract ② contains geraniol ≥75%, methyl salicylate ≥4.3%, citral ≥2.23%, and benzyl alcohol ≥10%. The Epiphyllum extract ③ is an extract obtained by oil extraction, and contains geraniol ≥ 66.5%, ocimene ≥ 6.35%, farnesene ≥ 2.3%, and pinene ≥ 1.25%. The Epiphyllum extract ④ is an extract obtained by steam distillation, and contains geraniol ≥ 58%, methyl salicylate ≥ 4.3%, citral ≥ 2.23%, benzyl alcohol ≥ 10%, and benzyl salicylate ≥ 1.22%. The mass fraction ratio of Epiphyllum extract ①: Epiphyllum extract ②: Epiphyllum extract ③: Epiphyllum extract ④ is 10~25:70~85:2~8:0.5~3; The epiphyllum extract is mixed with perfume-acceptable excipients, including at least one of solvents, stabilizers, and fragrance enhancers, and then the mixture is homogeneous to make a perfume.

2. The application according to claim 1, characterized in that, The steps for obtaining Epiphyllum extract① by low-temperature ethanol extraction are as follows: a1. Raw material harvesting: Select epiphyllum flowers in full bloom and harvest the petals between 10 pm and 2 am. Use a sterilized sharp tool to cut the petals 1-2 cm away from the plant. After harvesting, place them in a single layer in a clean and soft container, avoiding rainy days or nights with high humidity. b1. Immediately after harvesting, transfer the flowers to the processing workshop, remove diseased and damaged perianth pieces, and then preserve them with liquid nitrogen or dry ice. After quickly rinsing the perianth pieces with deionized water, absorb the moisture and cut them into small square pieces of 0.5±0.8cm. c1. Mix Epiphyllum oxypetalum and food-grade anhydrous ethanol at a mass-to-volume ratio of 1:6 to 1:9, put them into a brown glass bottle with a sealed cap, and extract at 4 to 8°C for 24 to 28 hours. d1. After extraction, filter the residue through a 300-mesh sieve. The filtrate is then rotary evaporated at 35~40℃ and -0.09~-0.1MPa until it becomes viscous. After dilution, filter the filtrate through a 0.22~0.28μm microporous membrane and collect the filtrate, which is the Epiphyllum extract①.

3. The application according to claim 1, characterized in that, The steps for obtaining Epiphyllum extract ② by supercritical CO2 extraction are as follows: a2. Raw material harvesting: Select epiphyllum flowers in full bloom and harvest the petals between 10 pm and 2 am. Use a sterilized sharp tool to cut the petals 1-2 cm away from the plant. After harvesting, place them in a single layer in a clean and soft container, avoiding rainy days or nights with high humidity. b2. Immediately after harvesting, transfer the flowers to the processing workshop, remove diseased and damaged perianth pieces, and then preserve them with liquid nitrogen or dry ice. After vacuum freeze-drying the perianth pieces at -50℃, pulverize them into 80-mesh powder. c2. The Epiphyllum powder in step b2 is extracted using supercritical CO2. The extraction parameters are set as follows: extraction pressure 30~35MPa, extraction temperature 35~39℃, CO2 flow rate 20~24L / h, and extraction time 2~2.5h. d2. Then, a two-stage separation method is used to remove impurities. The parameters for the first-stage separation are pressure 10~13MPa and temperature 40~43℃, and the parameters for the second-stage separation are pressure 5MPa and temperature 30~34℃. The separated extract is collected, which is the epiphyllum extract ②.

4. The application according to claim 1, characterized in that, The steps for obtaining Epiphyllum extract ③ by oil extraction are as follows: a3. Raw material harvesting: Select epiphyllum flowers in full bloom and harvest the tepals from 10 pm to 2 am. Use a sterilized sharp tool to cut the flower stalk 1-2 cm away from the plant. After harvesting, place them in a single layer in a clean and soft container, avoiding rainy days or nights with high humidity. b3. Immediately after harvesting, transfer the flowers to the processing workshop, remove diseased and damaged perianth pieces, and then preserve them with liquid nitrogen or dry ice. Wash and drain the perianth pieces and tear them into thin strips. c3. Use odorless liquid paraffin as a carrier, mix it with petals and carrier at a mass ratio of 1:4~5, put it into a sealed glass jar, and let it stand in a cool, dark place at 10~15℃ for 7 days for extraction. d3. After extraction, filter through a 200-mesh sieve to remove petal residue and collect the extract, which is the epiphyllum extract ③.

5. The application according to claim 1, characterized in that, The steps for obtaining Epiphyllum extract ④ by steam distillation are as follows: a4. Raw material harvesting: Select epiphyllum flowers in full bloom and harvest the tepals from 10 pm to 2 am. Use a sterilized sharp tool to cut the flower stalk 1-2 cm away from the plant. After harvesting, place them in a single layer in a clean and soft container, avoiding rainy days or nights with high humidity. b4. Immediately after harvesting, transfer the flowers to the processing workshop, remove any diseased or damaged perianth pieces, and then preserve them with liquid nitrogen or dry ice. Place the perianth pieces in a drying oven. c4. Dry the perianth pieces at 35~42℃ with forced air. Connect a condenser to the exhaust vent of the drying oven. After drying for 1~2 hours, start collecting the volatile flavor substances. Every 30~35 minutes, use a sterile pipette to transfer the collected extracts to a sterile container. d4. After collecting the extract, filter it immediately to remove impurities, then filter it through a 0.22~0.28μm microporous membrane, and then concentrate it under reduced pressure at 40~45℃ to obtain the concentrated solution, which is the epiphyllum extract ④.

6. The application according to claim 1, characterized in that: The sensory indicators of the Epiphyllum extract are as follows: clear and transparent in appearance, free of suspended matter and sediment, colorless or slightly yellow in color, and with the unique fragrance of Epiphyllum without any off-odor.

7. The application according to claim 1, characterized in that: The epiphyllum extract is stored according to any of the following methods: a) Add 0.02~0.03wt% vitamin E or rosemary extract, seal, and freeze at -18℃; b) Store in a dark, sealed glass bottle at a temperature below 20°C in a cool, dry place.

Images