Traditional Chinese medicine composition capable of regulating activity of pepsin and pancreatic lipase and preparation method thereof

Abstract

The invention discloses a traditional Chinese medicine composition capable of regulating activity of pepsin and pancreatic lipase and a preparation method thereof. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 1-2 parts of mint, 2-4 parts of galangal, 2-4 parts of liquorice, 3-6 parts of bran-fried fructus aurantii and 7-9 parts of Chinese dates. The traditional Chinese medicine composition has the effect of bidirectionally regulating the activity of digestive enzymes, on one hand, the activity of pepsin can be remarkably enhanced, and decomposition and digestion of protein in food are promoted; on the other hand, the activity of pancreatic lipase can be effectively inhibited, and decomposition and absorption of dietary fat in intestinal tracts are reduced.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of traditional Chinese medicine compositions, and particularly relates to a traditional Chinese medicine composition capable of adjusting the activities of pepsin and pancreatic lipase and a preparation method thereof. BACKGROUND

[0002] Pepsin is an important digestive protease secreted by the gastric mucosa of the stomach, and its main function is to decompose proteins in food into small peptide fragments. It is one of the key enzymes in the human protein digestion process. Pepsin is mainly used in the fields of pharmaceuticals, industry, food and feed, etc. Among them, the pharmaceutical industry is one of the main application fields of pepsin, especially in the production and research and development process of drugs. In different regions, the application fields of pepsin are also different. For example, in Europe, pepsin is widely used in the fields of inspection and quarantine, medicine and health supplements, etc.; while in developing countries, the downstream applications are mainly concentrated in the field of drugs.

[0003] Pancreatic lipase is a key digestive enzyme secreted by the pancreas, which is mainly responsible for breaking down fats (triglycerides) in food into smaller molecules (such as glycerol and fatty acids) for the body to absorb and utilize. It is one of the core enzymes of fat digestion and metabolism, and is widely distributed in the small intestine and pancreatic juice. In the application of inhibitors, Orlistat irreversibly binds to the active center of pancreatic lipase, inhibiting the absorption of 30% of dietary fat, with global annual sales exceeding 1.5 billion US dollars; natural inhibitors such as green tea polyphenols (EGCG) and mulberry leaf alkaloids have a growth rate of 20% / year in the Asian health product market due to their higher safety. SUMMARY

[0004] As one of the purposes of the present application, the present application provides a traditional Chinese medicine composition capable of adjusting the activities of pepsin and pancreatic lipase. The composition aims to optimize digestive function and assist in regulating fat intake, helping to more efficiently digest proteins (by promoting pepsin) and improve indigestion problems such as bloating and belching; at the same time, it mildly limits the absorption of part of the fat (by inhibiting pancreatic lipase), providing natural support for healthy weight management plans.

[0005] A traditional Chinese medicine composition capable of adjusting the activities of pepsin and pancreatic lipase, which is composed of the following raw materials in parts by weight: 1-2 parts of mint, 2-4 parts of high ginger, 2-4 parts of licorice, 3-6 parts of fried Schisandra chinensis and 7-9 parts of jujube.

[0006] Rhubarb is a processed product of traditional Chinese medicine, which is made from the dried immature fruits of Citrus aurantium and its cultivated varieties. It tastes bitter, pungent and sour, and is cold in nature, belonging to the meridians of the spleen and stomach. Rhubarb contains flavonoids (hesperidin, naringin), volatile oil (d-limonene) and alkaloids (synephrine), and has the effects of regulating gastrointestinal motility, anti-ulcer, anti-thrombosis, etc.

[0007] High-quality ginger is the dried rhizome of Alpinia officinarum, mainly produced in Guangdong and Hainan. It is pungent and hot, and belongs to the spleen and stomach channels. It is a classic medicine for warming the center and dispelling cold. It contains high-quality ginger, quercetin, and volatile oil (1,8-cineole), and has antibacterial, anticoagulant, and anti-ulcer effects.

[0008] Glycyrrhiza uralensis, a perennial herb of the Leguminosae family, is known as "national old" because of its unique sweet root. It is one of the most widely used traditional Chinese medicines. Its roots and rhizomes are used as medicine, with a neutral taste and a sweet flavor, and are associated with the heart, lung, spleen and stomach channels. It has the effects of tonifying the spleen and qi, clearing heat and detoxifying, relieving cough and phlegm, relieving pain and regulating various drugs, and is widely used to treat spleen and stomach weakness, cough and phlegm, sore throat and relieve drug toxicity.

[0009] Mentha canadensis L. is a perennial herb of the Lamiaceae family, known as "natural cooling agent" because of its unique cooling aroma and wide medicinal value. Its stems are straight and four-sided, its leaves are opposite, ovate-lanceolate or elliptical, with serrated edges, its flowers are light purple, and its fruits are small, oval-shaped nuts. It is cool and pungent, and belongs to the lung and liver channels. It has the effects of dispersing wind-heat, clearing the eyes, benefiting the throat, and relieving measles, and is widely used to treat wind-heat cold, headache, sore throat, measles, and liver stagnation.

[0010] Ziziphus jujuba Mill. is a deciduous small tree or shrub of the family Rhamnaceae, native to China and widely cultivated in Asia, Europe and the Americas. It is sweet and warm in nature, and belongs to the spleen and stomach channels. It is believed in traditional Chinese medicine to have the effects of tonifying the middle and replenishing qi, nourishing blood and calming the mind, and strengthening the spleen and stomach, and can regulate drug properties and relieve spleen and stomach weakness, blood deficiency, insomnia and other symptoms.

[0011] Preferably, the traditional Chinese medicine composition consists of the following raw materials by weight: 1.3-1.8 parts of mint, 2.5-3.8 parts of high-quality ginger, 2.5-3.8 parts of licorice, 3.5-5.5 parts of rhubarb and 7.5-9 parts of jujube.

[0012] As another inventive purpose of the present application, the present application also provides a preparation method of the traditional Chinese medicine composition, comprising the following steps:

[0013] (1) Extraction: 1-2 parts of mint, 2-4 parts of hokkaido ginger, 2-4 parts of licorice, 3-6 parts of Poria cocos and 7-9 parts of jujube are crushed, 10 times of 75% ethanol solution is added, and ultrasonic extraction is carried out for 2-3 times at 480-500W, 80-90min each time, and then cooled to room temperature, filtered, and the filtrate is combined;

[0014] (2) Concentration and drying: the filtered extract is concentrated and dried under reduced pressure and vacuum;

[0015] (3) Preparation and purification: the above extract is dissolved with 10 times of 75% ethanol, adsorbed on an AB-8 resin column for 2 hours, eluted with 10 times of ultrapure water for 1 hour, 10 times of 35% ethanol for 1 hour, 10 times of 75% ethanol for 1 hour, and 95% ethanol for 1 hour, and the eluate is collected;

[0016] (4) Concentration and drying: the collected 75% ethanol and ultrapure water eluate is concentrated and dried under reduced pressure and vacuum.

[0017] Preferably, the drying temperature in steps (2) and (4) is 45-50℃.

[0018] Compared with the prior art, the present application has the following advantages:

[0019] 1. Simple process and small side effects: compared with other digestion-promoting products, the present application uses a combination of natural products without the need for synthesis and structural modification, reducing the occurrence of side effects in the human body. At the same time, no additional auxiliary drugs are needed, maintaining the pure natural origin of the ingredients.

[0020] 2. Bidirectional regulation: providing differentiated digestive support, strongly promoting pepsin activity to solve protein digestion problems; selectively inhibiting pancreatic lipase to reduce the metabolic burden caused by high-fat diets. Helping the digestive system to more evenly process different nutrients. DETAILED DESCRIPTION

[0021] The present application will be further described below through examples.

[0022] The traditional Chinese medicine composition prepared by the examples and comparative examples is subjected to the following steps based on high-throughput screening of pancreatic lipase lipid-lowering:

[0023] Based on 4-methylumbelliferyl oleate (4-MUO) in the pig pancreatic lipase enzyme (PPL) under the action of defatting 4-methylumbelliferone (4-MU), using microplate reader to determine the dynamic fluorescence (λem=450nm) value of 4-MU at 320nm excitation wavelength, according to the fluorescence emission intensity to determine the inhibition ability of medicinal materials on enzyme.

[0024] 1. Solution preparation:

[0025] (1) Trichloroacetic acid solution (0.4 mol / L): accurately weigh 65.4 g of trichloroacetic acid, add deionized water to 1000 mL.

[0026] (2) Sodium carbonate solution (0.4 mol / L): accurately weigh 42.4 g of anhydrous sodium carbonate, add deionized water to dissolve, and then dilute to 1000 mL.

[0027] (3) Sodium hydroxide solution (0.1 mol / L): accurately weigh 0.4 g of sodium hydroxide, add deionized water to 100 mL.

[0028] (4) Hydrochloric acid solution (0.1 mol / L): take 8.33 mL of concentrated hydrochloric acid (12 mol / L) in a volumetric flask, add deionized water to 100 mL.

[0029] (5) Pepsin solution (stored at 4℃): accurately weigh 100 mg of pepsin, add 0.1 mol / L HCl solution to 10 mL.

[0030] (6) 2% Casein substrate buffer (stored at 4℃): accurately weigh 2 g of casein, add 40 mL of 0.1 mol / L sodium hydroxide, heat to dissolve in a water bath (40℃), and then dilute to 100 mL with deionized water.

[0031] (7) Tyrosine standard solution (stored at 4℃): accurately weigh 100 mg of tyrosine, add 0.1 M HCl solution to completely dissolve, then add deionized water to 100 mL to obtain a 1 mg / mL tyrosine stock solution. Before use, dilute with deionized water to 10, 20, 40, 80, 160, and 200 μg / mL for 6 gradients.

[0032] (8) Folin phenol solution purchased from MacLin P824172, concentration is 1N.

[0033] (9) Tris-HCl buffer solution: 500 mL of 0.1 mol / L Tris solution and 292 mL of 0.1 mol / L HCl were mixed, and then diluted to 1000 mL with ultrapure water to obtain a 0.05M Tris-HCl stock solution. Then 250 mL of 0.05M Tris-HCl stock solution was taken, 8.766 g of NaCl and 144.3 mg of CaCl2 were added, and then dissolved, and then diluted to 1 000 mL with ultrapure water.

[0034] (10) Pancreatic lipase (PPL) (30 U / mL): The pancreatic lipase powder was dissolved in the Tris-HCl buffer solution, and then filtered with a 0.45 μm microporous membrane, and the filtrate was collected.

[0035] (11) 4-MUO solution (0.1 mmol / L): 1 mg of 4-MUO was dissolved in 22.7 mL of DMSO solution.

[0036] 2. Experimental operation and calculation

[0037] (1) Pepsin digestion detection method:

[0038] Set up blank group BLK, sample group SPL, sample control group SPLC and positive control group STD

[0039] BLK / SPL: Take 80 μL of blank (water) and sample in a 1.5 mL centrifuge tube, add 80 μL of casein solution and 80 μL of pepsin solution, mix, and then incubate at 40°C for 20 min, then add 160 μL of trichloroacetic acid solution and mix, and then centrifuge for 5 min, take 80 μL of supernatant and add to a 96-well plate, then add 20 μL of folin phenol solution and 100 μL of sodium carbonate solution, incubate at 40°C for 20 min, and then read the absorbance value A at 680 nm BLK / A SPL . Each group sets 3 parallel samples.

[0040] SPLC: Take 80 μL of centrifuged sample in a 96-well plate, add 20 μL of ultrapure water and 100 μL of sodium carbonate solution, incubate at 40°C for 20 min, and then read the absorbance value A at 680 nm SPLC . Each group sets 3 parallel samples.

[0041] STD: Take 80 μL of 10, 20, 40, 80, 160, 200 μM tyrosine standard solution in a 96-well plate, add 20 μL of folin phenol solution and 100 μL of sodium carbonate solution, incubate at 40°C for 20 min, and then read the absorbance value A at 680 nm STD . Each group sets 3 parallel samples.

[0042] The 96-well plate was placed in an ELISA reader for detection, and the procedure is shown in Table 1:

[0043] Table 1

[0044]

[0045] Pepsin activity (u) = A SPL *K*N*0.2 / 20

[0046] A SPL 1 is the sample absorbance; K is the absorbance constant (calculated from the standard curve, the amount of tyrosine (μg) when the absorbance is 1 is the absorbance constant K value); N is the dilution factor (10 in this experiment); 0.2 is the reaction volume of 0.2 mL; 20 is the reaction time of 20 min.

[0047] (2) Method for detecting pancreatic lipase to lower lipid levels:

[0048] The reaction wells were set as follows: control well (BLK), blank control well (EMP), sample well (SPL), sample control well (SPLC), and standard well (STD). 150 μL of 12.5 mmol / L Tris-HCl buffer (pH 8.0), 10 μL of sample solution, and 30 μL of 30 U / mL pancreatic lipase were added to a clear 96-well plate. The plate was incubated at 37°C for 10 min in a microplate reader, followed by the rapid addition of 10 μL of 0.1 mmol / L 4-MUO solution. Measurements were then started immediately (see table for specific well loading details). Fluorescence intensity was recorded using the microplate reader software. The initial fluorescence intensity was recorded as f0, and subsequent measurements were taken every minute, with the intensities recorded as f1, f2, f3…f71. The net area under the fluorescence curve for the pancreatic lipase inhibitor was calculated by subtracting the integrated area under the sample control curve from the integrated area under the fluorescence curve for the pancreatic lipase inhibitor. AUC The net area of ​​the blank curve (Net BLK) is obtained by subtracting the area under the blank control fluorescence curve from the area under the blank fluorescence curve. AUC The net area of ​​the blank curve (Net STD) is obtained by subtracting the area under the fluorescence curve of the standard from the area under the fluorescence curve of the blank control. AUC ).

[0049] The corresponding sample addition wells are shown in Table 2:

[0050] Table 2

[0051] Tris-HCl (pH 8.0) PPL 4-MUO SPL Orlistat Loading concentration 12.5 mmol / L 30 U / mL 0.1 mmol / L nmol / L V 150 30 10 10 10 BLK 150+10 30 10 0 0 Emp 150+40 0 10 0 0 SPL / STD 150 30 10 10 0 SPLC 150+30 0 10 10 0

[0052] The plate reading program for the ELISA reader is shown in Table 3:

[0053] Table 3

[0054]

[0055] Net BLK AUC =AUC Blk -AUC Emp

[0056] Net SPL AUC =AUC SPL -AUC SPLC

[0057] The inhibition rate of the sample (%) = (Net BLK) AUC -Net SPL AUC ) / Net BLK AUC *100%

[0058] Example 1

[0059] This embodiment provides a method for preparing a traditional Chinese medicine composition with modifiable pepsin and pancreatic lipase activity, comprising the following steps:

[0060] (1) Extraction: The following components in parts by weight were pulverized: peppermint 1 part, galangal 2 parts, licorice 2 parts, stir-fried bitter orange peel 6 parts, and jujube 9 parts. 10 times the volume of 75% ethanol solution was added, and the mixture was ultrasonically extracted twice at 480W for 80 minutes each time. After extraction, the mixture was cooled to room temperature, filtered, and the filtrates were combined. Concentration and drying: The collected fraction was concentrated and dried under reduced pressure at 45℃.

[0061] (2) Preparation and purification: Dissolve the above extract in 10 times 75% ethanol, adsorb on AB-8 resin column for 2 hours, elute with 10 times ultrapure water for 0.5 hours, elute with 10 times 35% ethanol for 0.5 hours, elute with 10 times 75% ethanol for 1 hour, elute with 95% ethanol for 1 hour, and collect the eluent.

[0062] (3) Sample preparation: After the above-collected 75% ethanol and ultrapure water eluent is concentrated and dried under vacuum, it is dissolved in 10 times the amount of 75% ethanol.

[0063] Pepsin digestive function test: See above for specific operation and calculation. The pepsin activity promotion rate of this combination was 113.6%.

[0064] Pancreatic lipase lipid-lowering function test: See above for specific operation and calculation. The pancreatic lipase activity inhibition rate of this combination was 51.7%.

[0065] Example 2

[0066] This embodiment provides a method for preparing a traditional Chinese medicine composition with modifiable pepsin and pancreatic lipase activity, comprising the following steps:

[0067] (1) Extraction: The following components by weight were pulverized: peppermint 1.5 parts, galangal 2.5 parts, licorice 2.5 parts, stir-fried bitter orange peel 4.5 parts, and jujube 9 parts. 10 times the volume of 75% ethanol solution was added, and the mixture was ultrasonically extracted twice at 480W for 80 minutes each time. After extraction, the mixture was cooled to room temperature, filtered, and the filtrates were combined. Concentration and drying: The collected fraction was concentrated and dried under reduced pressure at 45℃.

[0068] (2) Preparation and purification: Dissolve the above extract in 10 times 75% ethanol, adsorb on AB-8 resin column for 2 hours, elute with 10 times ultrapure water for 0.5 hours, elute with 10 times 35% ethanol for 0.5 hours, elute with 10 times 75% ethanol for 1 hour, elute with 95% ethanol for 1 hour, and collect the eluent.

[0069] (3) Sample preparation: After the above-collected 75% ethanol and ultrapure water eluent is concentrated and dried under vacuum, it is dissolved in 10 times the amount of 75% ethanol.

[0070] Pepsin digestive function test: See above for specific operation and calculation. The pepsin activity promotion rate of this combination was 109.3%.

[0071] Pancreatic lipase lipid-lowering function test: See above for specific operation and calculation. The pancreatic lipase activity inhibition rate of this combination was 55.2%.

[0072] Example 3

[0073] This embodiment provides a method for preparing a traditional Chinese medicine composition with modifiable pepsin and pancreatic lipase activity, comprising the following steps:

[0074] (1) Extraction: The following components in parts by weight were pulverized: peppermint 2 parts, galangal 4 parts, licorice 4 parts, stir-fried bitter orange peel 3 parts, and jujube 7 parts. 10 times the volume of 75% ethanol solution was added, and the mixture was ultrasonically extracted twice at 480W for 80 minutes each time. After extraction, the mixture was cooled to room temperature, filtered, and the filtrates were combined. Concentration and drying: The collected fraction was concentrated and dried under reduced pressure at 45℃.

[0075] (2) Preparation and purification: Dissolve the above extract in 10 times 75% ethanol, adsorb on AB-8 resin column for 2 hours, elute with 10 times ultrapure water for 0.5 hours, elute with 10 times 35% ethanol for 0.5 hours, elute with 10 times 75% ethanol for 1 hour, elute with 95% ethanol for 1 hour, and collect the eluent.

[0076] (3) Sample preparation: After the above-collected 75% ethanol and ultrapure water eluent is concentrated and dried under vacuum, it is dissolved in 10 times the amount of 75% ethanol.

[0077] Pepsin digestive function test: See above for specific operation and calculation. The pepsin activity promotion rate of this combination was 114.7%.

[0078] Pancreatic lipase lipid-lowering function test: see above for specific operation and calculation. The pancreatic lipase activity inhibition rate of this combination is 65%.

[0079] Example 4

[0080] This embodiment provides a method for preparing a traditional Chinese medicine composition with modifiable pepsin and pancreatic lipase activity, comprising the following steps:

[0081] (1) Extraction: The following components by weight were pulverized: 2 parts peppermint, 4 parts galangal, 4 parts licorice, 6 parts stir-fried bitter orange peel, and 9 parts jujube. 10 times the volume of 75% ethanol solution was added, and the mixture was ultrasonically extracted twice at 480W for 80 minutes each time. After extraction, the mixture was cooled to room temperature, filtered, and the filtrates were combined. Concentration and drying: The collected fraction was concentrated and dried under reduced pressure at 45℃.

[0082] (2) Preparation and purification: Dissolve the above extract in 10 times 75% ethanol, adsorb on AB-8 resin column for 2 hours, elute with 10 times ultrapure water for 0.5 hours, elute with 10 times 35% ethanol for 0.5 hours, elute with 10 times 75% ethanol for 1 hour, elute with 95% ethanol for 1 hour, and collect the eluent.

[0083] (3) Sample preparation: After the above-collected 75% ethanol and ultrapure water eluent is concentrated and dried under vacuum, it is dissolved in 10 times the amount of 75% ethanol.

[0084] Pepsin digestive function test: See above for specific operation and calculation. The pepsin activity promotion rate of this combination is 120.3%.

[0085] Pancreatic lipase lipid-lowering function test: See above for specific operation and calculation. The pancreatic lipase activity inhibition rate of this combination was 50.9%.

[0086] Example 5

[0087] This embodiment provides a method for preparing a traditional Chinese medicine composition with modifiable pepsin and pancreatic lipase activity, comprising the following steps:

[0088] (1) Extraction: The following components in parts by weight were pulverized: peppermint 1 part, galangal 2 parts, licorice 2 parts, stir-fried bitter orange peel 3 parts, and jujube 7 parts. 10 times the volume of 75% ethanol solution was added, and the mixture was ultrasonically extracted twice at 480W for 80 minutes each time. After extraction, the mixture was cooled to room temperature, filtered, and the filtrates were combined. Concentration and drying: The collected fraction was concentrated and dried under reduced pressure at 45℃.

[0089] (2) Preparation and purification: Dissolve the above extract in 10 times 75% ethanol, adsorb on AB-8 resin column for 2 hours, elute with 10 times ultrapure water for 0.5 hours, elute with 10 times 35% ethanol for 0.5 hours, elute with 10 times 75% ethanol for 1 hour, elute with 95% ethanol for 1 hour, and collect the eluent.

[0090] (3) Sample preparation: After the above-collected 75% ethanol and ultrapure water eluent is concentrated and dried under vacuum, it is dissolved in 10 times the amount of 75% ethanol.

[0091] Pepsin digestive function test: See above for specific operation and calculation. The pepsin activity promotion rate of this combination was 106.6%.

[0092] Pancreatic lipase lipid-lowering function test: See above for specific operation and calculation. The pancreatic lipase activity inhibition rate of this combination was 51.7%.

[0093] Comparative Example 1

[0094] This comparative example provides a method for preparing a traditional Chinese medicine composition with modifiable pepsin and pancreatic lipase activity, comprising the following steps:

[0095] (1) Extraction: The following components by weight were pulverized: 2 parts peppermint, 4 parts galangal, 4 parts licorice, and 9 parts jujube. 10 times the volume of 75% ethanol solution was added, and the mixture was ultrasonically extracted twice at 480W for 80 minutes each time. After extraction, the mixture was cooled to room temperature, filtered, and the filtrates were combined. Concentration and drying: The collected fraction was concentrated and dried under reduced pressure at 45℃.

[0096] (2) Preparation and purification: Dissolve the above extract in 10 times 75% ethanol, adsorb on AB-8 resin column for 2 hours, elute with 10 times ultrapure water for 0.5 hours, elute with 10 times 35% ethanol for 0.5 hours, elute with 10 times 75% ethanol for 1 hour, elute with 95% ethanol for 1 hour, and collect the eluent.

[0097] (3) Sample preparation: After the above-collected 75% ethanol and ultrapure water eluent is concentrated and dried under vacuum, it is dissolved in 10 times the amount of 75% ethanol.

[0098] Pepsin digestive function test: See above for specific operation and calculation. The pepsin activity promotion rate of this combination is 100.3%.

[0099] Pancreatic lipase lipid-lowering function test: See above for specific operation and calculation. The pancreatic lipase activity inhibition rate of this combination was 38.9%.

[0100] Compared with Example 4, in Comparative Example 1, the pepsin activity promotion rate decreased from 120.3% to 100.3% and the pancreatic lipase activity inhibition rate decreased from 50.9% to 38.9% after the absence of stir-fried Citrus aurantium.

[0101] Comparative Example 2

[0102] This comparative example provides a method for preparing a traditional Chinese medicine composition with modifiable pepsin and pancreatic lipase activity, comprising the following steps:

[0103] (1) Extraction: The following components by weight were pulverized: 1 part peppermint, 2 parts galangal, 2 parts licorice, and 6 parts stir-fried bitter orange peel. 10 times the volume of 75% ethanol solution was added, and the mixture was ultrasonically extracted twice at 480W for 80 minutes each time. After extraction, the mixture was cooled to room temperature, filtered, and the filtrates were combined. Concentration and drying: The collected fraction was concentrated and dried under reduced pressure at 45℃.

[0104] (2) Preparation and purification: Dissolve the above extract in 10 times 75% ethanol, adsorb on AB-8 resin column for 2 hours, elute with 10 times ultrapure water for 0.5 hours, elute with 10 times 35% ethanol for 0.5 hours, elute with 10 times 75% ethanol for 1 hour, elute with 95% ethanol for 1 hour, and collect the eluent.

[0105] (3) Sample preparation: After the above-collected 75% ethanol and ultrapure water eluent is concentrated and dried under vacuum, it is dissolved in 10 times the amount of 75% ethanol.

[0106] Pepsin digestive function test: See above for specific operation and calculation. The pepsin activity promotion rate of this combination was 104.6%.

[0107] Pancreatic lipase lipid-lowering function test: See above for specific operation and calculation. The inhibition rate of pancreatic lipase activity of this combination was 28.1%.

[0108] Compared to Example 1, in Comparative Example 2, the pepsin activity promotion rate decreased from 113.6% to 104.6% after the absence of jujube. The pancreatic lipase activity inhibition rate decreased from 51.7% to 28.1%.

[0109] Comparative Example 3

[0110] This comparative example provides a method for preparing a traditional Chinese medicine composition with modifiable pepsin and pancreatic lipase activity, comprising the following steps:

[0111] (1) Extraction: The following components by weight were pulverized: 4 parts Alpinia officinarum, 4 parts Glycyrrhiza uralensis, and 3 parts Citrus aurantium stir-fried with wheat bran. 10 times the volume of 75% ethanol solution was added, and the mixture was ultrasonically extracted twice at 480W for 80 minutes each time. After extraction, the mixture was cooled to room temperature, filtered, and the filtrates were combined. Concentration and drying: The collected fraction was concentrated and dried under reduced pressure at 45℃.

[0112] (2) Preparation and purification: Dissolve the above extract in 10 times 75% ethanol, adsorb on AB-8 resin column for 2 hours, elute with 10 times ultrapure water for 0.5 hours, elute with 10 times 35% ethanol for 0.5 hours, elute with 10 times 75% ethanol for 1 hour, elute with 95% ethanol for 1 hour, and collect the eluent.

[0113] (3) Sample preparation: After the above-collected 75% ethanol and ultrapure water eluent is concentrated and dried under vacuum, it is dissolved in 10 times the amount of 75% ethanol.

[0114] Pepsin digestive function test: See above for specific operation and calculation. The pepsin activity promotion rate of this combination was 74.8%.

[0115] Pancreatic lipase lipid-lowering function test: See above for specific operation and calculation. The inhibition rate of pancreatic lipase activity of this combination was 36.5%.

[0116] Compared to Example 3, in Comparative Example 3, the pepsin activity promotion rate decreased from 114.7% to 74.8% after the removal of peppermint and jujube herbs. The pancreatic lipase activity inhibition rate decreased from 65% to 36.5%.

[0117] Comparative Example 4

[0118] This comparative example provides a method for preparing a traditional Chinese medicine composition with modifiable pepsin and pancreatic lipase activity, comprising the following steps:

[0119] (1) Extraction: The following components by weight were pulverized: 4 parts licorice, 6 parts stir-fried immature bitter orange peel, and 9 parts jujube. 10 times the volume of 75% ethanol solution was added, and the mixture was ultrasonically extracted twice at 480W for 80 minutes each time. After extraction, the mixture was cooled to room temperature, filtered, and the filtrates were combined. Concentration and drying: The collected fraction was concentrated and dried under vacuum at a temperature of 45℃.

[0120] (2) Preparation and purification: Dissolve the above extract in 10 times 75% ethanol, adsorb on AB-8 resin column for 2 hours, elute with 10 times ultrapure water for 0.5 hours, elute with 10 times 35% ethanol for 0.5 hours, elute with 10 times 75% ethanol for 1 hour, elute with 95% ethanol for 1 hour, and collect the eluent.

[0121] (3) Sample preparation: After the above-collected 75% ethanol and ultrapure water eluent is concentrated and dried under vacuum, it is dissolved in 10 times the amount of 75% ethanol.

[0122] Pepsin digestive function test: See above for specific operation and calculation. The pepsin activity promotion rate of this combination is 93%.

[0123] Pancreatic lipase lipid-lowering function test: See above for specific operation and calculation. The inhibition rate of pancreatic lipase activity of this combination was 20.5%.

[0124] Compared to Example 4, the pepsin activity promotion rate of Comparative Example 4 decreased from 120.3% to 93% after the absence of peppermint and galangal. The pancreatic lipase activity inhibition rate decreased from 50.9% to 20.5%.

[0125] Comparative Example 5

[0126] This comparative example provides a method for preparing a traditional Chinese medicine composition with modifiable pepsin and pancreatic lipase activity, comprising the following steps:

[0127] (1) Extraction: Weigh 10 portions of each of the 5 medicinal materials and pulverize them. Add 10 times the volume of 75% ethanol solution and extract twice with ultrasound at 450W for 80 minutes each time. After extraction, cool to room temperature, filter, and combine the filtrates. Concentration and drying: Concentrate and dry the collected fractions under reduced pressure at 45℃.

[0128] (2) Preparation and purification: Dissolve the above extract in 10 times 75% ethanol, adsorb on AB-8 resin column for 2 hours, elute with 10 times ultrapure water for 1 hour, elute with 10 times 35% ethanol for 1 hour, elute with 10 times 75% ethanol for 1 hour, elute with 95% ethanol for 2 hours, and collect the eluent.

[0129] (3) Sample preparation: After the above-collected 75% ethanol and ultrapure water eluent is concentrated and dried under vacuum, it is dissolved in 10 times the amount of 75% ethanol (to ensure that the concentration of each sample of the same composition is 0.1 g / mL).

[0130] Detection of pepsin-induced digestion and pancreatic lipase-induced lipid-lowering function:

[0131] Peppermint: Pepsin promotion rate: 36.56%, pancreatic lipase inhibition rate: 19.99%.

[0132] Alpinia galanga: Pepsin promotion rate: 79.62%, pancreatic lipase inhibition rate: 22.55%.

[0133] Licorice: Pepsin promotion rate: 20.53%, pancreatic lipase inhibition rate: 28.37%.

[0134] Fried Citrus aurantium: Pepsin promotion rate: 85.08%, Pancreatic lipase inhibition rate: 12.67%.

[0135] Jujube: Pepsin promotion rate: 99.05%, pancreatic lipase inhibition rate: 34.6%.

[0136] Comparative Example 7

[0137] This comparative example provides a method for preparing a traditional Chinese medicine composition with modifiable pepsin and pancreatic lipase activity, comprising the following steps:

[0138] (1) Extraction: The following components in parts by weight were pulverized: peppermint 1 part, galangal 2 parts, licorice 2 parts, stir-fried bitter orange peel 6 parts, and jujube 9 parts. 10 times the volume of 75% ethanol solution was added, and the mixture was ultrasonically extracted twice at 480W for 80 minutes each time. After extraction, the mixture was cooled to room temperature, filtered, and the filtrates were combined. Concentration and drying: The collected fraction was concentrated and dried under reduced pressure at 45℃.

[0139] (2) Preparation and purification: Dissolve the above extract in 10 times 75% ethanol, adsorb on AB-8 resin column for 2 hours, elute with 10 times ultrapure water for 1 hour, elute with 10 times 35% ethanol for 1 hour, elute with 10 times 75% ethanol for 1 hour, elute with 95% ethanol for 2 hours, and collect the eluent.

[0140] (3) Sample preparation: After the above-collected 35% ethanol and 75% ethanol eluents are concentrated and dried under vacuum, they are dissolved in 10 times the amount of 75% ethanol.

[0141] Pepsin digestive function test: See above for specific operation and calculation. The pepsin activity promotion rate of this combination was 79.8%.

[0142] Pancreatic lipase lipid-lowering function test: See above for specific operation and calculation. The inhibition rate of pancreatic lipase activity of this combination was 24.7%.

[0143] Compared to Example 1, after the sample preparation steps of Comparative Example 7 were changed to combine 35% and 75% components, the pepsin activity promotion rate decreased from 113.6% to 79.8%. The pancreatic lipase activity inhibition rate decreased from 51.7% to 24.7%.

[0144] Comparative Example 8

[0145] This comparative example provides a method for preparing a traditional Chinese medicine composition with modifiable pepsin and pancreatic lipase activity, comprising the following steps:

[0146] (1) Extraction: The following components in parts by weight were pulverized: peppermint 1 part, galangal 2 parts, licorice 2 parts, stir-fried bitter orange peel 6 parts, and jujube 9 parts. 10 times the volume of 75% ethanol solution was added, and the mixture was ultrasonically extracted twice at 480W for 80 minutes each time. After extraction, the mixture was cooled to room temperature, filtered, and the filtrates were combined. Concentration and drying: The collected fraction was concentrated and dried under reduced pressure at 45℃.

[0147] (2) Preparation and purification: Dissolve the above extract in 10 times 75% ethanol, adsorb on AB-8 resin column for 2 hours, elute with 10 times ultrapure water for 1 hour, elute with 10 times 35% ethanol for 1 hour, elute with 10 times 75% ethanol for 1 hour, elute with 95% ethanol for 2 hours, and collect the eluent.

[0148] (3) Sample preparation: After the above-collected 35% ethanol and ultrapure water eluent is concentrated and dried under vacuum, it is dissolved in 10 times the amount of 75% ethanol.

[0149] Pepsin digestive function test: See above for specific operation and calculation. The pepsin activity promotion rate of this combination was 66.4%.

[0150] Pancreatic lipase lipid-lowering function test: See above for specific operation and calculation. The inhibition rate of pancreatic lipase activity of this combination was 23.4%.

[0151] Compared to Example 1, after the sample preparation steps of Comparative Example 8 were changed to include 35% and ultrapure water components, the pepsin activity promotion rate decreased from 113.6% to 66.4%. The pancreatic lipase activity inhibition rate decreased from 51.7% to 23.4%.

[0152] Comparative Example 9

[0153] This comparative example provides a method for preparing a traditional Chinese medicine composition with modifiable pepsin and pancreatic lipase activity, comprising the following steps:

[0154] (1) Extraction: The following components in parts by weight were pulverized: peppermint 1 part, galangal 2 parts, licorice 2 parts, stir-fried bitter orange peel 6 parts, and jujube 9 parts. 10 times the volume of 75% ethanol solution was added, and the mixture was ultrasonically extracted twice at 480W for 80 minutes each time. After extraction, the mixture was cooled to room temperature, filtered, and the filtrates were combined. Concentration and drying: The collected fraction was concentrated and dried under reduced pressure at 45℃.

[0155] (2) Preparation and purification: Dissolve the above extract in 10 times 75% ethanol, adsorb on AB-8 resin column for 2 hours, elute with 10 times ultrapure water for 1 hour, elute with 10 times 35% ethanol for 1 hour, elute with 10 times 75% ethanol for 1 hour, elute with 95% ethanol for 2 hours, and collect the eluent.

[0156] (3) Sample preparation: After the ultrapure water eluent collected above is concentrated and dried under vacuum, it is dissolved in 10 times the amount of 75% ethanol.

[0157] Pepsin digestive function test: See above for specific operation and calculation. The pepsin activity promotion rate of this combination was 43.4%.

[0158] Pancreatic lipase lipid-lowering function test: See above for specific operation and calculation. The inhibition rate of pancreatic lipase activity of this combination was 19.7%.

[0159] Compared to Example 1, after the sample preparation steps of Comparative Example 9 were changed to use only water, the pepsin activity promotion rate decreased from 113.6% to 43.4%. The pancreatic lipase activity inhibition rate decreased from 51.7% to 19.7%.

[0160] Comparative Example 10

[0161] This comparative example provides a method for preparing a traditional Chinese medicine composition with modifiable pepsin and pancreatic lipase activity, comprising the following steps:

[0162] (1) Extraction: The following components in parts by weight were pulverized: peppermint 1 part, galangal 2 parts, licorice 2 parts, stir-fried bitter orange peel 6 parts, and jujube 9 parts. 10 times the volume of 75% ethanol solution was added, and the mixture was ultrasonically extracted twice at 480W for 80 minutes each time. After extraction, the mixture was cooled to room temperature, filtered, and the filtrates were combined. Concentration and drying: The collected fraction was concentrated and dried under reduced pressure at 45℃.

[0163] (2) Preparation and purification: Dissolve the above extract in 10 times 75% ethanol, adsorb on AB-8 resin column for 2 hours, elute with 10 times ultrapure water for 1 hour, elute with 10 times 35% ethanol for 1 hour, elute with 10 times 75% ethanol for 1 hour, elute with 95% ethanol for 2 hours, and collect the eluent.

[0164] (3) Sample preparation: After the above-collected 75% ethanol eluent is concentrated and dried under vacuum, it is dissolved in 10 times the amount of 75% ethanol.

[0165] Pepsin digestive function test: See above for specific operation and calculation. The pepsin activity promotion rate of this combination was 61.9%.

[0166] Pancreatic lipase lipid-lowering function test: See above for specific operation and calculation. The inhibition rate of pancreatic lipase activity of this combination was 23.2%.

[0167] Compared to Example 1, after the sample preparation steps of Comparative Example 10 were changed to a single 75% component, the pepsin activity promotion rate decreased from 113.6% to 561.9%. The pancreatic lipase activity inhibition rate decreased from 51.7% to 23.2%.

[0168] Comparative Example 11

[0169] This comparative example provides a method for preparing a traditional Chinese medicine composition with modifiable pepsin and pancreatic lipase activity, comprising the following steps:

[0170] (1) Extraction: The following components by weight were pulverized: 3 parts peppermint, 1 part galangal, 1 part licorice, 7 parts stir-fried bitter orange peel, and 4 parts jujube. Ten times the volume of 75% ethanol solution was added, and the mixture was ultrasonically extracted twice at 480W for 80 minutes each time. After extraction, the mixture was cooled to room temperature, filtered, and the filtrates were combined. Concentration and drying: The collected fraction was concentrated and dried under reduced pressure at 45℃.

[0171] (2) Preparation and purification: Dissolve the above extract in 10 times 75% ethanol, adsorb on AB-8 resin column for 2 hours, elute with 10 times ultrapure water for 1 hour, elute with 10 times 35% ethanol for 1 hour, elute with 10 times 75% ethanol for 1 hour, elute with 95% ethanol for 2 hours, and collect the eluent.

[0172] (3) Sample preparation: After the above-collected 35% ethanol eluent is concentrated and dried under vacuum, it is dissolved in 10 times the amount of 75% ethanol.

[0173] Pepsin digestive function test: See above for specific operation and calculation. The pepsin activity promotion rate of this combination was 95.6%.

[0174] Pancreatic lipase lipid-lowering function test: see above for specific operation and calculation. The inhibition rate of pancreatic lipase activity of this combination was 33.4%.

[0175] Compared to Example 1, the sample in Comparative Example 11 was not within the scope of the combination of the present invention, and the pepsin activity promotion rate decreased from 113.6% to 95.6%. The pancreatic lipase activity inhibition rate decreased from 51.7% to 33.4%.

[0176] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A traditional Chinese medicine composition having the ability to regulate the activity of pepsin and pancreatic lipase, characterized in that, The ingredients are composed of the following ingredients by weight: 1-2 parts peppermint, 2-4 parts galangal, 2-4 parts licorice, 3-6 parts stir-fried bitter orange peel, and 7-9 parts jujube.

2. The traditional Chinese medicine composition according to claim 1, characterized in that, The traditional Chinese medicine composition consists of the following raw materials in parts by weight: 1.3-1.8 parts of peppermint, 2.5-3.8 parts of galangal, 2.5-3.8 parts of licorice, 3.5-5.5 parts of stir-fried immature bitter orange peel, and 7.5-9 parts of jujube.

3. A method for preparing the traditional Chinese medicine composition according to claim 1 or 2, characterized in that, Includes the following steps: (1) Extraction: By weight, 1-2 parts of peppermint, 2-4 parts of galangal, 2-4 parts of licorice, 3-6 parts of stir-fried bitter orange peel and 7-9 parts of jujube were crushed and added with 10 times 75% ethanol solution. The mixture was ultrasonically extracted 2-3 times at 480-500W for 80-90 minutes each time. After extraction, the mixture was cooled to room temperature, filtered, and the filtrates were combined. (2) Concentration and drying: The filtered extract is concentrated and dried under reduced pressure and vacuum. (3) Preparation and purification: Dissolve the above extract in 10 times 75% ethanol, adsorb on AB-8 resin column for 2 hours, elute with 10 times ultrapure water for 1 hour, elute with 10 times 35% ethanol for 1 hour, elute with 10 times 75% ethanol for 1 hour, elute with 95% ethanol for 1 hour, and collect the eluent. (4) Concentration and drying: The 75% ethanol and ultrapure water eluent collected above is concentrated and dried under reduced pressure and vacuum to obtain the final product.

4. The method according to claim 3, characterized in that, The drying temperature in steps (2) and (4) is 45-50℃.

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