Culture dish and use method thereof

Abstract

The invention relates to the technical field of culture equipment, in particular to a culture dish and a using method.The culture dish comprises a culture dish body, the culture dish body comprises a culture dish cover and a culture dish bottom, a culture area is arranged in the culture dish bottom, and an auxiliary module is detachably installed on the culture dish body; at least one auxiliary dish plug is installed on the circumferential side of the auxiliary module, at least one culture dish plug is arranged on the circumferential side of the culture dish body, an overflow groove is formed in the contact face of the top of the culture dish bottom and the culture dish cover, and the bottom of the culture dish bottom is of a concave surface structure. Meanwhile, the invention discloses a use method based on the culture dish, by adopting the culture dish and the use method, free combination can be carried out according to needs, the culture dish can meet the needs of culture with different characteristics, and the convenience is improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of culture equipment, in particular to a culture dish and a use method thereof. BACKGROUND

[0002] As a core carrier for biological sample experiments, the selection of the type and structure of the culture dish directly affects the accuracy and reliability of the experimental results. Different biological samples have significant differences in metabolic methods, growth environment requirements, morphological characteristics and experimental purposes, so it is necessary to match the characteristics of the culture dish and equip different types and structures of the culture dish, which causes inconvenience to the operators. SUMMARY

[0003] The purpose of the present application is to provide a culture dish and a use method thereof, which solve the above technical problems.

[0004] To achieve the above purpose, the present application provides a culture dish, which comprises a culture dish body, the culture dish body comprises a culture dish cover and a culture dish bottom, a culture area is arranged in the culture dish bottom, the culture dish body is detachably installed with an auxiliary module, at least one auxiliary dish plug is installed on the circumferential side of the auxiliary module, at least one culture dish plug is arranged on the circumferential side of the culture dish body, an overflow groove is arranged on the contact surface between the top of the culture dish bottom and the culture dish cover, and the bottom of the culture dish bottom is a concave structure.

[0005] Preferably, the culture dish bottom is provided with a culture dish mounting hole on the circumferential side, an annular groove A is arranged in the culture dish mounting hole, a culture dish plug is arranged in the culture dish mounting hole, and an annular protrusion A matched with the annular groove A is arranged on the circumferential side of the culture dish plug, so as to realize the interference fit installation of the culture dish plug in the culture dish mounting hole. A positioning dish plug mounting hole matched with the convex skirt of the culture dish plug is arranged on the circumferential side of the culture dish bottom at a position opposite to the culture dish mounting hole.

[0006] Preferably, the culture dish plug comprises a culture dish plug plug, a culture dish one-way valve plug and a culture dish gas permeable membrane plug. The culture dish bottom is a sealed culture dish bottom or a gas permeable culture dish bottom, the gas permeable culture dish bottom is provided with a gas permeable through hole between the two culture dish mounting holes on the circumferential side, and a culture dish gas permeable membrane is arranged on the inner side of the culture area at a position opposite to the gas permeable through hole, the culture dish gas permeable membrane is a hydrophobic microporous filter membrane. The culture dish plug plug is a solid structure, and the culture dish plug plug is made of polycarbonate, polypropylene, polystyrene or acrylonitrile-butadiene-styrene copolymer material. The petri dish one-way valve plug is hollow, made of silica gel or rubber, and has a strip-shaped notch or opening at one end facing the culture area for one-way flow of medium, which is in a sealed state under no medium pressure and in an open state under medium pressure. The petri dish gas-permeable membrane plug is hollow, made of polycarbonate, polypropylene, polystyrene or acrylonitrile-butadiene-styrene copolymer, and has a plug gas-permeable membrane installed at one end opposite the culture area for gas exchange, which is a hydrophobic microporous filter membrane.

[0007] Preferably, the sealed petri dish is combined with the petri dish plug to be used for fully sealed culture, combined with the petri dish one-way valve plug for sealed culture under liquid change, combined with the petri dish gas-permeable membrane plug for sealed culture under the first anaerobic flux gas exchange, and combined with the petri dish plug, the petri dish one-way valve plug and the petri dish gas-permeable membrane plug for sealed culture under the second anaerobic flux gas exchange. Preferably, the petri dish bottom is combined with the petri dish plug for gas-permeable sealed culture, combined with the petri dish one-way valve plug for sealed culture under liquid change, combined with the petri dish gas-permeable membrane plug for sealed culture under the first aerobic flux gas exchange, and combined with the petri dish plug, the petri dish one-way valve plug and the petri dish gas-permeable membrane plug for sealed culture under the second aerobic flux gas exchange.

[0008] Preferably, the petri dish mounting table for connecting the moving parts is further included, the petri dish bottom is provided with an inclined angle clamping groove A, the petri dish mounting table is provided with an inclined angle clamping groove B matched with the inclined angle clamping groove A, the petri dish mounting table is provided with a positioning boss, and the petri dish bottom is provided with a bottom positioning groove matched with the positioning boss.

[0009] Preferably, the auxiliary module includes an auxiliary sealing cover, an auxiliary bottom and an auxiliary plug, the auxiliary bottom is provided with a storage unit made of foamed material for storing water or carbon dioxide, the storage unit is placed in a storage limiting groove of the auxiliary bottom, the auxiliary sealing cover is provided at the bottom with an inclined angle clamping groove C and a direction limiting protrusion, the auxiliary bottom is provided at the top with an inclined angle clamping groove D and a direction limiting groove, the inclined angle clamping groove C and the inclined angle clamping groove D are in interference fit, both the inclined angle clamping groove C and the inclined angle clamping groove D are polygonal, and the direction limiting protrusion and the direction limiting groove are in clearance fit to realize clamping of the auxiliary sealing cover and the auxiliary bottom.

[0010] Preferably, the circumferential side of the auxiliary bottom is provided with an auxiliary mounting hole, which is a through hole; the auxiliary mounting hole is symmetrically provided with a trapezoidal positioning hole for limiting the installation direction and position of the auxiliary dish plug; The outer side of the auxiliary dish plug is provided with an annular protrusion B, which is matched with the annular groove A in the dish mounting hole; the circumferential side of the auxiliary dish plug is symmetrically provided with a trapezoidal positioning structure, which is clearance-fitted with the trapezoidal positioning hole; the edge of the trapezoidal positioning structure is provided with anti-skid lines; The auxiliary dish plug includes an auxiliary module one-way valve dish plug and an auxiliary module gas permeable membrane dish plug. The auxiliary module gas permeable membrane dish plug is provided with an auxiliary gas permeable membrane on the opposite side of the culture area, which covers the gas exchange port; the circumferential side of the auxiliary module gas permeable membrane dish plug is provided with an air inlet; the outer side of the auxiliary module gas permeable membrane dish plug is provided with an air outlet, which has a smaller diameter than the gas exchange port. The inner side of the auxiliary sealing cover is provided with circumferentially distributed positioning teeth, which are oppositely arranged with the central axis reference of the auxiliary mounting hole; the top of the culture dish bottom is provided with a top positioning groove, which is oppositely arranged with the central axis reference of the culture dish mounting hole; the top positioning groove is correspondingly arranged with the positioning teeth. The upper end surface of the auxiliary sealing cover is concave and provided with circumferentially distributed drainage port groups; each group of drainage ports includes two mirror-image arranged drainage ports A and B, which are provided with a separation structure; the drainage ports A and B are through holes with an angle of 135° with the upper end surface of the auxiliary sealing cover. The bottom of the auxiliary bottom is provided with an inclined angle clamping groove E, which is interference-fitted with an inclined angle clamping groove F of the auxiliary mounting table; both the inclined angle clamping groove E and the inclined angle clamping groove F are three-section structures. The auxiliary bottom is provided with an inclined angle clamping groove G, which is interference-fitted with an inclined angle clamping groove A.

[0011] Preferably, the culture dish cover and the culture dish bottom are threadedly connected; the culture dish cover is provided with at least one pair of clamping protrusions matched with the U-shaped clamping grooves of the cover opener; the U-shaped clamping grooves of the cover opener are arranged on the clamping protrusions to rotate the culture dish cover.

[0012] Preferably, the culture dish cover and the culture dish bottom are interference-fitted; the embedded part of the culture dish cover has a larger size than the inner side of the culture area; the top of the culture dish bottom is provided with a shrinkable reinforcing rib.

[0013] Based on the use method of the above culture dish, the specific steps are as follows: Step S1: selecting the auxiliary dish plug and the culture dish plug according to the characteristics of the culture; Step S2: open the culture dish cover, add culture medium until the liquid surface of the culture medium is flush with the lowest surface of the overflow groove; Step S3: transplant the culture sample to the culture area, and install the culture dish cover; Step S4: place the culture dish in the culture instrument for culture.

[0014] Therefore, the culture dish and the use method have the beneficial effects that the culture dish body and the auxiliary module are combined according to actual needs, and different types of auxiliary dish plugs and culture dish plugs are determined according to the ventilation needs, so that various combinations are realized to adapt to the needs of different culture environments.

[0015] The technical solutions of the present application will be further described in detail below with the help of the drawings and examples. BRIEF DESCRIPTION OF DRAWINGS

[0016] Figure 1 It is a structure diagram of the culture dish of the present application; Figure 2 It is an exploded view of the culture dish body of the present application; Figure 3 It is a sectional view of the culture dish installation table connected to the culture dish of the present application; Figure 4 It is a structure diagram of the bottom of the breathable culture dish; Figure 5 It is a structure diagram of the plug of the culture dish; Figure 6 It is a structure diagram of the one-way valve plug of the culture dish; Figure 7 It is a sectional view of the breathable film plug of the culture dish; Figure 8 It is an exploded view of the auxiliary module; Figure 9 It is a sectional view of the combination of the culture dish body and the auxiliary module; Figure 10 It is an assembly diagram of the auxiliary installation table; Figure 11 It is a structure diagram of the auxiliary sealing cover; Figure 12 It is a structure diagram of the auxiliary bottom; Figure 13 It is a structure diagram of the auxiliary installation table; Figure 14 It is a structure diagram of the one-way valve plug of the auxiliary module; Figure 15 It is a sectional view of the breathable film plug of the auxiliary module; Figure 16 It is a sectional view of the breathable film plug of the auxiliary module; Figure 17 It is a structure diagram of Example 2; Figure 18 schematic diagram of gas flow and liquid flow.

[0017] Reference signs 1, culture dish body; 11, culture dish cover; 111, card convex; 112, embedded part; 12, culture dish bottom; 121, breathable through hole; 122, culture dish breathable film; 123, bevel card slot A; 124, bottom positioning groove; 125, shrinkage reinforcing rib; 126, overflow groove; 127, top positioning groove; 13, culture area; 14, culture dish mounting hole; 141, annular groove A; 15, positioning dish plug mounting hole; 2, culture dish plug; 21, annular convex A; 22, convex skirt; 23, culture dish plug plug; 24, culture dish one-way valve plug; 241, strip-shaped cutout; 25, culture dish breathable film plug; 251, plug breathable film; 3, auxiliary tool; 31, cover opener; 311, U-shaped card slot; 32, drainage plug; 33, sealing plug; 4, auxiliary plug; 41, annular convex B; 42, trapezoidal positioning structure; 421, anti-skid pattern; 43, auxiliary module one-way valve plug; 44, auxiliary module breathable film plug; 441, auxiliary breathable film; 442, air exchange port; 443, air inlet; 444, air outlet hole; 5, auxiliary module; 51, auxiliary sealing cover; 511, bevel card slot C; 512, direction limiting convex; 513, positioning tooth; 514, drainage port A; 515, drainage port B; 516, separation structure; 517, flow guide groove; 52, auxiliary bottom; 521, storage limiting groove; 522, bevel card slot D; 523, direction limiting groove; 524, auxiliary mounting hole; 5241, trapezoidal positioning hole; 525, bevel card slot E; 526, bevel card slot G; 53, storage unit; 6, culture dish mounting table; 61, bevel card slot B; 62, positioning boss; 7, auxiliary mounting table; 71, bevel card slot F; 8, inner air duct. DETAILED DESCRIPTION

[0018] In the description of this invention, it should be noted that the terms "upper," "lower," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship commonly used when the product is in use. They are used only for the convenience of describing the invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the invention. In the description of this invention, it should also be noted that, unless otherwise explicitly specified and limited, the terms "set," "install," and "connect" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal communication between two components. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0019] The embodiments of the present invention will now be described in detail with reference to the accompanying drawings.

[0020] Example 1 like Figure 1 As shown, a petri dish includes a petri dish body 1, which includes a petri dish lid 11 and a petri dish bottom 12. A culture zone 13 is provided inside the petri dish bottom 12. The petri dish lid 11 and the petri dish bottom 12 are threaded together.

[0021] The auxiliary module 5 has a polygonal structure on the outside. In this embodiment, the auxiliary module 5 is a 12-sided prism structure. At least one culture dish plug 2 is provided on the circumference side of the culture dish. Three culture dish mounting holes 14 are opened on the circumference side of the bottom 12 of the culture dish. The culture dish mounting holes 14 are distributed at 120° intervals with the culture area 13 as the center, that is, they are distributed in a circle.

[0022] like Figures 2-3As shown, the culture dish mounting hole 14 is provided with an annular groove A141, the culture dish mounting hole 14 is provided with a culture dish plug 2, the circumferential side of the culture dish plug 2 is provided with an annular protrusion A21 matched with the annular groove A141, for realizing the interference fit installation of the culture dish plug 2 in the culture dish mounting hole 14, realizing the clamping of the culture dish plug 2 in the culture dish mounting hole 14 without loosening, and at the same time playing a sealing and leakage prevention role. The culture dish plug mounting hole and the culture dish plug 2 are both conical structures, when the annular protrusion A21 is arranged in the annular groove A141, it can ensure that the culture dish plug is in full contact with the inner wall of the culture dish mounting hole 14, and plays a sealing and leakage prevention role. The circumferential side of the culture dish bottom 12 is provided with a positioning plug mounting hole 15 at a position opposite to the culture dish mounting hole 14, the positioning plug mounting hole 15 is matched with the convex skirt 22 of the culture dish plug 2, and can assist in the clearance fit installation of the convex skirt 22 of the culture dish plug 2, preventing the culture dish plug 2 from being installed out of position. The culture dish plug 2 and the culture dish mounting hole 14 support repeated plugging and unplugging while still maintaining the sealing and leakage prevention effect.

[0023] The culture dish body 1 is detachably mounted with an auxiliary module 5, at least one auxiliary plug 4 is mounted on the circumferential side of the auxiliary module 5, and the top of the culture dish bottom 12 is provided with an overflow groove 126 on the contact surface of the culture dish cover 11. When the culture dish cover 11 is installed into the culture dish bottom 12 filled with liquid, the overflow groove 126 can temporarily store the liquid overflowing after the culture dish bottom 12 is installed with the culture dish cover 11, while improving the sealing effect.

[0024] The bottom of the culture dish bottom 12 is a concave structure, when the culture dish is placed horizontally, the sample contained in the culture area 13 can naturally gather at the lowest part of the concave structure, and the center point of the culture area 13 is the lowest position of the concave surface, solving the problem of adsorption of the culture sample on the inner wall of the culture area 13.

[0025] The culture dish plug 2 includes a culture dish plug 23, a culture dish one-way valve plug 24, and a culture dish gas permeable membrane plug 25, and the culture dish bottom 12 is a sealed culture dish bottom 12 or a gas permeable culture dish bottom 12, as shown. Figure 4 The culture dish plug 2 and the culture dish bottom 12 can be combined and adjusted according to actual needs.

[0026] As shown, Figure 5 The culture dish plug 23 is a solid structure, and the culture dish plug 23 is made of polycarbonate, polypropylene, polystyrene or acrylonitrile-butadiene-styrene copolymer material.

[0027] As shown, Figure 6As shown, the petri dish one-way valve plug 24 is a hollow structure, and the petri dish one-way valve plug 24 is made of silica gel or rubber. The end of the one-way valve plug facing the culture area 13 is provided with a strip-shaped cutout 241 or an opening, which has a one-way valve function. When there is no medium pressure, the strip-shaped cutout 241 or the opening is in a sealed state, and when subjected to medium pressure, it is in an open state, allowing fluid medium or hard pipeline to pass through the strip-shaped cutout 241 or the opening in a single direction from the B port (the end facing the outside) to the A port (the end provided with the strip-shaped cutout 241). It can prevent the fluid medium in the culture area 13 from being output from the A port to the B port. If a pipeline is inserted into the B port of the petri dish one-way valve plug 24 in transition fit or interference fit with the petri dish one-way valve plug 24 for fluid input, or a pipeline is inserted into the A port of the petri dish one-way valve plug 24 in transition fit or interference fit with the petri dish one-way valve plug 24 for fluid output, the petri dish body 1 can be placed in any direction, and the pipeline can be used for inputting or outputting fluid, which can play a sealing and leakage prevention role.

[0028] As shown in Figure 7 The petri dish gas-permeable membrane plug 25 is a hollow structure, and the petri dish gas-permeable membrane plug 25 is made of polycarbonate, polypropylene, polystyrene, or acrylonitrile-butadiene-styrene copolymer. The petri dish gas-permeable membrane plug 25 is installed with a plug gas-permeable membrane 251 at the end opposite to the culture area 13 for gas exchange. The plug gas-permeable membrane is a hydrophobic microporous filter membrane, and the hydrophobic microporous filter membrane is made of PTFE polytetrafluoroethylene or PVDF polyvinylidene fluoride filter membrane with a pore size of 0.22 µm or 0.45 µm. The petri dish body 1 is placed vertically on the horizontal plane with the petri dish mounting hole 14 facing upward, and the petri dish gas-permeable membrane plug 25 in the upward direction is removed to support the culture area 13 for liquid exchange operation.

[0029] The sealed petri dish is used in combination with the petri dish plug plug 23 for fully sealed culture. When performing liquid exchange operation, the petri dish body 1 is placed vertically on the horizontal plane with the petri dish mounting hole 14 facing upward, and the petri dish plug plug 23 in the upward direction is removed to support the culture area 13 for liquid exchange operation. It is applied to anaerobic microbial culture and can withstand high-frequency vibration without leakage, and is suitable for long-distance sample transfer.

[0030] The sealed petri dish is used in combination with the petri dish one-way valve plug 24 for sealed culture in a sealed state (without disassembling the petri dish plug 2 and the petri dish cover 11). It can effectively prevent the culture medium and the culture sample from being exposed to the operating environment, and can effectively reduce and prevent the leakage of pathogens when applied to high-pathogenicity microbial culture, thereby protecting personnel and the environment.

[0031] The sealing culture dish is used in combination with the culture dish gas-permeable film plug 25 for sealed culture with first anaerobic flux aeration, which can realize free exchange of low-flux gas (such as oxygen, carbon dioxide and water vapor), and is suitable for culture of facultative anaerobic microorganisms.

[0032] The culture dish plug plug 23, the culture dish one-way valve plug 24 and the culture dish gas-permeable film plug 25 are used in combination with the sealing culture dish (one of each of the culture dish plug plug 23, the culture dish one-way valve plug 24 and the culture dish gas-permeable film plug 25 is used), for sealed culture with liquid exchange in the second anaerobic flux aeration state, and three kinds of culture dish plugs 2 are used simultaneously in combination with a plurality of culture dish gas-permeable film plugs 25, the gas-permeable flux is lower, and the culture with lower aeration requirement is suitable, and the liquid exchange is convenient and fast.

[0033] The gas-permeable culture dish bottom 12 is provided with a gas-permeable through hole 121 between the two culture dish mounting holes 14 on the circumferential side, and the culture dish gas-permeable film 122 is arranged at the position opposite to the gas-permeable through hole 121 inside the culture area 13, and is installed on the inner circular side wall of the culture area 13 with the center of the culture area 13 as the circumferential center with an interval of 120°, and covers the gas-permeable through hole 121. It is used for free exchange of gas (such as oxygen, carbon dioxide and water vapor), while effectively blocking the entry and exit of liquid (culture medium) and microorganisms. The culture dish gas-permeable film 122 is a hydrophobic microporous filter film, and the hydrophobic microporous filter film adopts a PTFE polytetrafluoroethylene or PVDF polyvinylidene fluoride material filter film with a pore size of 0.22µm or 0.45µm.

[0034] The gas-permeable culture dish bottom 12 is used in combination with the culture dish plug plug 23 for gas-permeable sealed culture; it can realize the purpose of sealed culture, and is suitable for culture of aerobic microorganisms, such as aerobic bacteria, fungi, mammalian cells and embryonic stem cells; it can tolerate low-frequency vibration without leakage, and is suitable for long-distance transfer of samples.

[0035] The gas-permeable culture dish bottom 12 is used in combination with the culture dish one-way valve plug 24 for sealed culture with liquid exchange in a gas-permeable state; it can realize the purpose of sealed culture, and is suitable for culture of aerobic microorganisms, such as aerobic bacteria, fungi, mammalian cells and embryonic stem cells; it can realize liquid addition (culture medium) and liquid exchange without disassembling the culture dish plug 2 and the culture dish cover 11, and can effectively prevent the culture medium and the culture sample from being exposed to the operating environment, and can effectively reduce and prevent leakage of pathogens when used for culture of highly pathogenic microorganisms, thereby protecting personnel and the environment.

[0036] The air-permeable culture dish bottom 12 is used in combination with the culture dish air-permeable film plug 25 to seal the culture dish in a sealed state for first aerobic flux aeration; it can realize free exchange of large flux gas (such as oxygen, carbon dioxide and water vapor), while effectively blocking the entry and exit of liquid (culture medium) and microorganisms, and is suitable for aerobic microbial culture, such as aerobic bacteria, fungi, mammalian cells and embryonic stem cell culture.

[0037] The culture dish plug 23, the culture dish one-way valve plug 24 and the culture dish air-permeable film plug 25 are used in combination with the air-permeable culture dish bottom 12 to seal the culture dish in a sealed state for liquid exchange in a second aerobic flux aeration state, and the second aerobic flux is lower than the first aerobic flux.

[0038] It also includes a culture dish mounting table 6 for connecting moving parts, in Figure 3 The culture dish bottom 12 is provided with an inclined angle clamping groove A123 at the bottom, the culture dish mounting table 6 is provided with an inclined angle clamping groove B61 matched with the inclined angle clamping groove A123, the inclined angle clamping groove A123 and the inclined angle clamping groove B61 realize the buckle type installation of the culture dish body 1 and the culture dish mounting table 6, support repeated plugging and fixing effect, the culture dish mounting table 6 is provided with a positioning boss 62, and the culture dish bottom 12 is provided with a bottom positioning groove 124 matched with the positioning boss 62, which can cooperate with the boss of the culture dish mounting table 6 to realize installation positioning. The culture dish body 1 can move with the culture dish mounting table 6 (such as three-axis shaking motion, two-axis shaking motion and rotary motion, etc.), which is suitable for dynamic motion and suspension culture, such as stem cell culture, organoid culture and cell tissue culture.

[0039] As shown in Figure 8 The auxiliary module 5 includes an auxiliary sealing cover 51, an auxiliary bottom 52 and an auxiliary plug 4, and the auxiliary bottom 52 is provided with a storage unit 53.

[0040] The storage unit 53 is a foamed material (sponge or wood pulp cotton, etc.), which is used for storing water or carbon dioxide (carbon dioxide is dissolved in water to form carbonic acid); the storage unit 53 is placed in the storage limiting groove 521 of the auxiliary bottom 52 and is between the adjacent two auxiliary mounting holes 524, and three storage units 53 are arranged.

[0041] As shown in Figures 9-10As shown, the bottom of the auxiliary sealing cover 51 is provided with an inclined angle clamping groove C511 and a direction limiting protrusion 512, and the top of the auxiliary bottom 52 is provided with an inclined angle clamping groove D522 and a direction limiting recess 523. The inclined angle clamping groove C511 and the inclined angle clamping groove D522 are in interference fit, and both of them are polygons (12-gon). The 12-gon edges are the assembly position limiting points of the auxiliary sealing cover 51 and the auxiliary bottom 52, which can ensure that the auxiliary sealing cover 51 and the auxiliary bottom 52 are not deviated during assembly. The direction limiting protrusion 512 and the direction limiting recess 523 are in clearance fit. The direction limiting protrusion 512 is arranged opposite to the axis of the auxiliary mounting hole 524, which can effectively limit the installation of the auxiliary sealing cover 51 on the auxiliary bottom 52 according to the fixed design installation position, and is arranged opposite to the auxiliary mounting hole 524 to realize the clamping of the auxiliary sealing cover 51 and the auxiliary bottom 52 without loosening and shaking.

[0042] The circumferential side of the auxiliary bottom 52 is provided with three auxiliary mounting holes 524, which are uniformly distributed around the center point of the auxiliary bottom 52, and the auxiliary mounting hole 524 is arranged on the edge of the 12-gon. The auxiliary mounting hole 524 is a through hole; and the auxiliary mounting hole 524 is symmetrically provided with a trapezoidal positioning hole 5241 for limiting the installation direction and position of the auxiliary dish plug 4. The outer side of the auxiliary dish plug 4 is provided with an annular protrusion B41 which is matched with the annular recess A141 in the culture dish mounting hole 14, so that the auxiliary dish plug 4 can be clamped into the culture mounting hole, and the sealing and leakage prevention effects are achieved without loosening. The auxiliary dish plug 4 is designed in a conical structure to improve the sealing performance and prevent leakage. The circumferential side of the auxiliary dish plug 4 is symmetrically provided with a trapezoidal positioning structure 42 which is in clearance fit with the trapezoidal positioning hole 5241 to limit the installation position and direction (0 and 180°) of the auxiliary dish plug 4. The edge of the trapezoidal positioning structure 42 is provided with an anti-skid pattern 421 to facilitate the embedding of the installation gap of the tool (such as a one-word screwdriver with a head width less than 4.0mm). The tool can be embedded in the installation gap between the trapezoidal positioning hole 5241 and the trapezoidal positioning structure 42. The anti-skid pattern 421 improves the friction provided by the contact of the tool end with the trapezoidal positioning structure 42. The tool can take out the auxiliary dish plug 4 from the culture mounting hole and the auxiliary mounting hole 524 through the principle of lever. The auxiliary dish plug 4 and the culture dish plug 2 support repeated plugging and unplugging while still maintaining the sealing and leakage prevention effects.

[0043] The auxiliary dish plug 4 includes an auxiliary module one-way valve dish plug 43 and an auxiliary module gas permeable membrane dish plug 44. Figure 14 、 Figure 15 and Figure 16As shown, the auxiliary module petri dish one-way valve plug 43 is similar in structure to the petri dish one-way valve plug 24, and the auxiliary module petri dish one-way valve plug 43 does not have a convex skirt 22, enabling one-way medium input. The auxiliary module gas-permeable membrane petri dish plug 44 is similar in structure to the petri dish gas-permeable membrane petri dish plug 25, and also does not have a convex skirt 22. The auxiliary module gas-permeable membrane petri dish plug 44 is provided with an auxiliary gas-permeable membrane 441 on the opposite side of the culture zone 13. The auxiliary gas-permeable membrane 441 covers a gas exchange port 442. The auxiliary module gas-permeable membrane petri dish plug 44 is provided with an air inlet 443 on the circumferential side. The outer side of the auxiliary module gas-permeable membrane petri dish plug 44 is provided with an air outlet hole 444, which has a smaller diameter than the gas exchange hole. The auxiliary gas-permeable membrane 441 enables free exchange of gases (such as oxygen, carbon dioxide, and water vapor), while effectively blocking the entry and exit of liquids (culture medium) and microorganisms.

[0044] The inner side of the auxiliary sealing cover 51 is provided with three positioning teeth 513 uniformly distributed in the circumference. The positioning teeth 513 are arranged opposite the central axis reference of the auxiliary mounting hole 524. The top of the petri dish bottom 12 is provided with three top positioning grooves 127, which are arranged opposite the central axis reference of the petri dish mounting hole 14. The top positioning grooves 127 correspond to the positioning teeth 513 and are installed through a transition fit. The positioning teeth 513 and the top positioning grooves 127 can indicate the positions of the petri dish mounting hole 14 and the auxiliary mounting hole 524, enabling the petri dish mounting hole 14 and the auxiliary mounting hole 524 to be installed in position, ensuring that the petri dish mounting hole 14 and the auxiliary mounting hole 524 are on the same central axis.

[0045] The upper end surface of the auxiliary sealing cover 51 is concave and provided with a circumferentially distributed drainage port group. When liquid substances are injected through the drainage port group, the concave surface of the auxiliary sealing cover 51 can effectively temporarily store the liquid substances that overflow the drainage port group during the injection process, effectively preventing the injected liquid substances from overflowing the auxiliary sealing cover 51. Each drainage port group includes two mirror-image drainage ports A 514 and B 515, which can allow gaseous and liquid substances to enter. The drainage port A 514 and the drainage port B 515 are provided with a partition structure 516, which is not through. The drainage port A 514 and the drainage port B 515 are through holes at an angle of 135° with the upper end surface of the auxiliary sealing cover 51. The auxiliary sealing cover 51 is designed with three drainage port groups, which are spaced apart at an angle of 120° with the center of the auxiliary sealing cover 51 as the reference, i.e., uniformly distributed, and the drainage port A 514 and the drainage port B 515 are arranged at an angle of 90°.

[0046] When the auxiliary sealing cover 51 is in irregular motion (non-rotational motion), airflow can enter the lower end surface of the auxiliary sealing cover 51 through the drainage port A 514 and the drainage port B 515.

[0047] When the auxiliary sealing cover 51 is rotated with the upper end surface as the reference surface, the process is as follows: When the auxiliary sealing cover 51 is in clockwise rotation, the airflow enters through the drainage port B515 on the upper end face of the auxiliary sealing cover 51 and is divided into two streams of airflow by the separation structure 516, the first stream of airflow overflowing from the upper end face of the auxiliary sealing cover 51 through the drainage port A514, and the second stream of airflow overflowing from the lower end face of the auxiliary sealing cover 51 through the drainage port B515.

[0048] When the auxiliary sealing cover 51 is in counterclockwise rotation, the airflow enters through the drainage port A514 on the upper end face of the auxiliary sealing cover 51 and is divided into two streams of airflow by the separation structure 516, the first stream of airflow overflowing from the upper end face of the auxiliary sealing cover 51 through the drainage port B515, and the second stream of airflow overflowing from the lower end face of the auxiliary sealing cover 51 through the drainage port A514; the drainage port structure design can ensure that the airflow can only flow into the lower end face of the auxiliary sealing cover 51 from the upper end face of the auxiliary sealing cover 51.

[0049] The direction limiting protrusion 512 is arranged opposite to the symmetry axes of the drainage ports A514 and B515, so that the drainage ports A514 and B515 are symmetrically installed with the auxiliary mounting hole 524 axis.

[0050] As shown in Figure 18 , when in motion, the storage unit 53 will approach the inner air duct 8 and the flow suppression inlet, so as to ensure that the gas (black arrow direction) can only enter from the flow suppression inlet → pass through the storage unit 53 → enter the inner air duct 8 (the gap between the culture dish bottom 12 and the auxiliary module 5) → enter the culture area 13 through the air inlet 443 or the air permeable through hole 121, so that the gas entering from the flow suppression inlet can be fully mixed with the water vapor in the storage unit 53 before entering the culture area 13.

[0051] When liquid is injected from the drainage ports A514 and B515 into the storage unit 53 (gray arrow direction), the liquid flows into the storage unit 53 through the flow guide groove 517, and the excess liquid can overflow to the storage limiting groove 521, which can effectively prevent the liquid from overflowing to the inner air duct 8 when the culture dish is placed flat.

[0052] As shown in Figure 11 , Figure 12 and Figure 13 , the auxiliary bottom 52 is provided with an inclined angle clamping groove E525 at the bottom, and the inclined angle clamping groove E525 is interference fit with the inclined angle clamping groove F71 of the auxiliary mounting table 7. The inclined angle clamping groove E525 and the inclined angle clamping groove F71 are both uniformly three-section structures and achieve buckle type installation, supporting repeated plugging and fixing, and can assist the inclined angle clamping groove E525 to be installed on the inclined angle clamping groove F71 at a predetermined position, realizing the fixing and installation positioning function of the auxiliary module 5.

[0053] The auxiliary base 52 is provided with an angled slot G526, which is interference-fitted with the angled slot A123 to realize the installation connection between the auxiliary module 5 and the culture dish. The angled slot G526 and the angled slot A123 are installed in an upside-down manner and do not support repeated insertion and removal.

[0054] It also includes an auxiliary tool 3 for opening and closing the petri dish lid 11. The auxiliary tool 3 includes a lid opener 31, a drainage plug 32, and a sealing plug 33. The lid opener 31 is a plate structure with U-shaped slots 311 at both ends. The petri dish lid 11 is provided with three pairs (six) of protrusions 111 that are adapted to the U-shaped slots 311. The three pairs (six) of protrusions 111 are evenly distributed around the circumference. The U-shaped slots 311 of the lid opener 31 are fitted with the protrusions 111 with a clearance. The lid opener 31 assists the operator in rotating the petri dish lid 11 to unscrew or tighten the petri dish lid 11 and install it on the bottom 12 of the petri dish.

[0055] Example 2 like Figure 17 As shown, the petri dish lid 11 and the petri dish bottom 12 are interference-fitted. Both the lid 11 and bottom are made of a shrinkable material, which ensures a tight seal. The embedded portion 112 of the lid 11 is larger than the inner dimension of the culture area 13. This interference fit effectively guarantees the sealing performance of the petri dish bottom 12 and lid 11. A shrinkable reinforcing rib 125 is provided on the top of the bottom 12. The shrinkable reinforcing rib 125 ensures a tight fit between the mounting surfaces of the bottom 12 and lid 11, thus guaranteeing a tight seal.

[0056] The method of using a culture dish is characterized by the following specific steps: Step S1: Select auxiliary stopper 4 and culture dish stopper 2 according to the characteristics of the culture; Step S2: Open the petri dish lid 11, add culture medium until the culture medium liquid level is flush with the lowest surface of the overflow tank 126; Step S3: Transfer the cultured sample to the culture area 13 and install the culture dish lid 11; Step S4: Place the petri dish in a culture instrument for incubation.

[0057] When the culture dish body 1, auxiliary module 5, and auxiliary module vented membrane plug 44 are used together, gas exchange with the external environment can only occur through the drainage port group and the vent holes 444 of the auxiliary module vented membrane plug 44. This enables free exchange of gases (such as oxygen, carbon dioxide, and water vapor) between the culture zone 13 and the outside environment, while effectively blocking the entry and exit of liquid (culture medium) and microorganisms in the culture zone 13.

[0058] When the combined culture dish is used in a non-movement state, gases (such as oxygen, carbon dioxide, and water vapor) enter the inside of the auxiliary module 5 through the drainage port group and the gas outlet hole 444 of the auxiliary module gas-permeable film dish plug 44 by the diffusion effect of the gases, the gases in the inside of the auxiliary module 5 enter the gas exchange hole through the gas inlet hole, and the culture area 13 exchanges gases through the auxiliary gas-permeable film 441 of the auxiliary module gas-permeable film dish plug 44, when the combined culture dish is used in a movement state (such as a rotating movement), the gases (such as oxygen, carbon dioxide, and water vapor) enter the auxiliary module 5 through the drainage port group, and the auxiliary module 5 forms a positive pressure, the gas outlet hole 444 of the gas-permeable film dish plug has a smaller diameter than the gas exchange hole, the gases enter the auxiliary module gas-permeable film dish plug 44 through the gas inlet hole, and a part of the gas flow forms a swirling gas flow in the gas exchange hole and then flows out from the gas outlet hole 444. The combined application can realize free exchange of gases between the culture area 13 and the inside and outside of the auxiliary module 5 in a static culture environment and a dynamic culture environment.

[0059] When the culture dish body 1, the auxiliary module 5, the auxiliary module gas-permeable film dish plug 44, the drainage plug 32, and the sealing plug 33 are combined for use, the drainage plug 32 is assembled on the drainage port group of the auxiliary sealing cover 51, and the sealing plug 33 is assembled on the gas outlet hole 444 of the auxiliary module gas-permeable film dish plug 44, so that the combined culture dish is in a fully sealed state. Water is supplied to the storage unit 53 through the drainage port group of the auxiliary sealing cover 51, and input gases (such as carbon dioxide gas with a concentration required for culture) are supplied to the storage unit 53 through the gas outlet hole 444, and after the supply is completed, the combined culture dish is sealed by using the drainage plug 32 and the sealing plug 33. Free exchange of gases between the culture area 13 and the inside of the auxiliary module 5 can be realized, which is suitable for maintaining a high-humidity and PH culture environment for a longer time when the sample is transferred for a long time, and is suitable for culture of facultative anaerobic microorganisms, such as culture of Escherichia coli, Saccharomyces cerevisiae, and Staphylococcus aureus.

[0060] When the culture dish body 1, the auxiliary module 5, and the auxiliary module one-way valve dish plug 43 are combined for use, free exchange of gases (such as oxygen, carbon dioxide, and water vapor) between the culture area 13 and the inside of the auxiliary module 5 can be realized, which is suitable for culture of aerobic microorganisms, such as culture of aerobic bacteria, fungi, mammalian cells, and embryonic stem cells. The auxiliary module one-way valve dish plug 43 and the culture dish cover can be realized without being disassembled, liquid (culture medium) addition and liquid (culture medium) replacement operations of the culture area 13 can be realized, and the culture medium and the culture sample can be effectively prevented from being exposed to the operating environment, which can effectively reduce and prevent leakage of pathogens when applied to culture of highly pathogenic microorganisms, and can protect personnel and the environment.

[0061] When the culture dish body 1, the auxiliary module 5, the auxiliary module one-way valve dish plug 43 and the auxiliary module gas permeable membrane dish plug 44 are used in combination, two auxiliary module one-way valve dish plugs 43 and one auxiliary module gas permeable membrane dish plug 44, or one auxiliary module one-way valve dish plug 43 and two auxiliary module gas permeable membrane dish plugs 44 are used, and the adjustment of the auxiliary dish plug 4 is made according to the actual situation.

[0062] When the culture dish body 1, the auxiliary module 5 and the auxiliary installation table 7 are used in combination, the culture dish body 1 is positioned and installed on the auxiliary module 5, and the culture dish body 1 has the functions of fixation and installation positioning while relying on the functions of fixation and installation positioning of the auxiliary module 5, and is suitable for dynamic motion and suspension culture, such as stem cell culture, organoid culture and cell tissue culture.

[0063] Finally, it should be noted that: the above examples are only used to illustrate the technical solutions of the present application rather than limiting them, and although the present application has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present application can still be modified or replaced by equivalents, and these modifications or equivalent replacements cannot make the modified technical solutions deviate from the spirit and scope of the technical solutions of the present application.

Claims

1. A petri dish comprising a petri dish body comprising a petri dish lid and a petri dish base, the petri dish base having a culture area disposed therein, characterised in that: The culture dish body is detachably installed with an auxiliary module, at least one auxiliary dish plug is installed at the circumferential side of the auxiliary module, at least one culture dish plug is arranged at the circumferential side of the culture dish body, the contact surface between the top of the culture dish bottom and the culture dish cover is provided with an overflow groove, and the bottom of the culture dish bottom is a concave structure.

2. A petri dish according to claim 1, wherein: The culture dish bottom is provided with a culture dish mounting hole at the circumferential side, an annular groove A is arranged in the culture dish mounting hole, a culture dish plug is arranged in the culture dish mounting hole, and an annular protrusion A matched with the annular groove A is arranged at the circumferential side of the culture dish plug, so as to realize the interference fit of the culture dish plug in the culture dish mounting hole. A positioning dish plug mounting hole is arranged at the circumferential side of the culture dish bottom and opposite to the culture dish mounting hole, and the positioning dish plug mounting hole is matched with the convex skirt of the culture dish plug.

3. The petri dish of claim 1, wherein: The culture dish plug comprises a culture dish plug head, a culture dish one-way valve plug and a culture dish gas permeable membrane plug. The culture dish bottom is a sealed culture dish bottom or a gas permeable culture dish bottom, the gas permeable culture dish bottom is provided with a gas permeable through hole between the two culture dish mounting holes at the circumferential side, and a culture dish gas permeable membrane is arranged at the position opposite to the gas permeable through hole in the culture area. The culture dish plug head is a solid structure, and the culture dish plug head is made of polycarbonate, polypropylene, polystyrene or acrylonitrile-butadiene-styrene copolymer material. The culture dish one-way valve plug is a hollow structure, and the culture dish one-way valve plug is made of silica gel or rubber material, one end of the one-way valve plug facing the culture area is provided with a strip-shaped notch or opening for one-way flow of medium, and the strip-shaped notch or opening is in a sealed state when there is no medium pressure and is in an open state when subjected to medium pressure. The culture dish gas permeable membrane plug is a hollow structure, and the culture dish gas permeable membrane plug is made of polycarbonate, polypropylene, polystyrene or acrylonitrile-butadiene-styrene copolymer material, a plug gas permeable membrane is arranged at the position opposite to the culture area of the culture dish gas permeable membrane plug for gas exchange, and the plug gas permeable membrane is a hydrophobic microporous filter membrane.

4. A petri dish according to claim 3, wherein: The sealed culture dish is used in combination with the culture dish plug head for fully sealed culture, the sealed culture dish is used in combination with the culture dish one-way valve plug for sealed culture under liquid exchange in a sealed state, the sealed culture dish is used in combination with the culture dish gas permeable membrane plug for sealed culture under first anaerobic flux gas exchange, and the culture dish plug head, the culture dish one-way valve plug and the culture dish gas permeable membrane plug are used in combination with the sealed culture dish for sealed culture under liquid exchange in a second anaerobic flux gas exchange state. The gas permeable culture dish bottom is used in combination with the culture dish plug head for gas permeable sealed culture, the gas permeable culture dish bottom is used in combination with the culture dish one-way valve plug for sealed culture under liquid exchange in a gas permeable state, the gas permeable culture dish bottom is used in combination with the culture dish gas permeable membrane plug for sealed culture under first aerobic flux gas exchange in a sealed state, and the culture dish plug head, the culture dish one-way valve plug and the culture dish gas permeable membrane plug are used in combination with the gas permeable culture dish bottom for sealed culture under liquid exchange in a second aerobic flux gas exchange state.

5. The petri dish of claim 1, wherein: The culture dish mounting table for connecting the moving parts is provided with an inclined angle clamping groove A at the bottom of the culture dish bottom, and the culture dish mounting table is provided with an inclined angle clamping groove B matched with the inclined angle clamping groove A.

6. The petri dish of claim 1, wherein: The auxiliary module includes an auxiliary sealing cover, an auxiliary bottom and an auxiliary dish plug, the auxiliary bottom is provided with a storage unit, the storage unit is a foamed material, and is used for storing water or carbon dioxide; the storage unit is placed in the storage limiting groove of the auxiliary bottom; the bottom of the auxiliary sealing cover is provided with an inclined angle clamping groove C and a direction limiting protrusion, the top of the auxiliary bottom is provided with an inclined angle clamping groove D and a direction limiting groove, the inclined angle clamping groove C and the inclined angle clamping groove D are in interference fit, the inclined angle clamping groove C and the inclined angle clamping groove D are both polygonal, the direction limiting protrusion and the direction limiting groove are in clearance fit, and the auxiliary sealing cover and the auxiliary bottom are clamped.

7. A petri dish according to claim 6, wherein: The circumference side of the auxiliary bottom is provided with an auxiliary mounting hole, and the auxiliary mounting hole is a through hole; the auxiliary mounting hole is provided with a trapezoidal positioning hole in symmetry, and is used for limiting the installation direction and position of the auxiliary dish plug; The outer side of the auxiliary dish plug is provided with an annular protrusion B matched with an annular groove A in the culture dish mounting hole, the circumference side of the auxiliary dish plug is provided with a trapezoidal positioning structure in symmetry, the trapezoidal positioning structure is in clearance fit with the trapezoidal positioning hole, and the edge of the trapezoidal positioning structure is provided with an anti-skid pattern; The auxiliary dish plug includes an auxiliary module one-way valve dish plug and an auxiliary module breathable membrane dish plug; The auxiliary module breathable membrane dish plug is provided with an auxiliary breathable membrane opposite to the culture area, the auxiliary breathable membrane covers the air exchange port, the circumference side of the auxiliary module breathable membrane dish plug is provided with an air inlet, and the outer side of the auxiliary module breathable membrane dish plug is provided with an air outlet hole, the diameter of the air outlet hole is smaller than that of the air exchange hole; The inner side of the auxiliary sealing cover is provided with a circumferentially distributed positioning tooth, the positioning tooth is opposite to the central axis reference of the auxiliary mounting hole, the top of the culture dish bottom is provided with a top positioning groove opposite to the central axis reference of the culture dish mounting hole, and the top positioning groove is correspondingly provided with the positioning tooth; The upper end surface of the auxiliary sealing cover is provided with an inner concave surface and circumferentially distributed drainage port groups, each drainage port group includes two mirror image arranged drainage ports A and B, and a separation structure is arranged between the drainage port A and the drainage port B; the drainage port A and the drainage port B are through holes with an angle of 135° with the upper end surface of the auxiliary sealing cover; The bottom of the auxiliary bottom is provided with an inclined angle clamping groove E, and the inclined angle clamping groove E is in interference fit with an inclined angle clamping groove F of the auxiliary mounting table; the inclined angle clamping groove E and the inclined angle clamping groove F are both three-section structures; The auxiliary bottom is provided with an inclined angle clamping groove G in interference fit with the inclined angle clamping groove A.

8. The petri dish of claim 1, wherein: The culture dish cover and the culture dish bottom are in interference fit, the embedded part of the culture dish cover is larger than the inner side size of the culture area, and the top of the culture dish bottom is provided with a shrinkable reinforcing rib.

9. The petri dish of claim 1, wherein: The specific steps are as follows:

10. Use of a petri dish according to any one of claims 1-9, characterized in that, ​ Step S1: Selecting the auxiliary dish plug and the dish plug according to the characteristics of the culture; Step S2: Opening the dish cover, adding the culture medium until the liquid surface of the culture medium is flush with the lowest surface of the overflow groove; Step S3: Transplanting the culture sample to the culture area and installing the dish cover; Step S4: Placing the dish in the culture instrument for culture.

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