An ultrasonic cell disruptor

Abstract

This invention provides an ultrasonic cell disruptor, belonging to the field of cell disruption technology. The disruptor includes a working chamber, an ultrasonic probe, and a support within the working chamber. The support is mounted on the lower part of the ultrasonic probe, and a receiving opening is provided on the support, with a container slidably connected to the opening. This invention solves the problem in existing ultrasonic cell disruptors where, during cell disruption, the container holding the cell solution to be disrupted is placed directly below the ultrasonic probe. The vibrations generated during cell disruption easily cause displacement of the container, leading to a deviation between the cell solution and the focal area of ​​the ultrasonic probe, thus reducing the efficiency of the cavitation effect.

Description

Technical Field

[0001] This invention belongs to the field of cell disruption technology, specifically relating to an ultrasonic cell disruptor. Background Technology

[0002] An ultrasonic cell disruptor converts electrical energy into acoustic energy via a transducer. This energy is then transformed into dense small bubbles through a liquid medium. These small bubbles rapidly burst, thereby disrupting cells and other substances.

[0003] Existing ultrasonic cell disruptors place the container of cell fluid to be disrupted directly below the ultrasonic probe during cell disruption. The vibrations generated during cell disruption can easily cause displacement of the container, leading to a deviation between the cell fluid and the focal area of ​​the ultrasonic probe, thus reducing the efficiency of the cavitation effect. Summary of the Invention

[0004] This invention provides an ultrasonic cell disruptor, which aims to solve the problem that existing ultrasonic cell disruptors, when disrupting cells, place the container containing the cell fluid to be disrupted directly below the ultrasonic probe. The vibration generated during cell disruption can easily cause displacement of the container, leading to a deviation between the cell fluid and the focal area of ​​the ultrasonic probe, thus reducing the efficiency of the cavitation effect.

[0005] This invention provides an ultrasonic cell disruptor, comprising a cell disruptor with a pre-reserved working chamber, an ultrasonic probe mounted on the cell disruptor, a support mounted in the working chamber, the support being mounted on the lower part of the ultrasonic probe, and a pre-reserved receiving opening on the support, with a container slidably connected to the receiving opening.

[0006] Preferably, the holding component includes a holding block one, a holding block two is fitted to the lower part of the holding block one, elastic coverings are fitted to the outer walls of the holding block one and the holding block two, the elastic coverings are in close contact with the inner wall of the receiving opening, a linkage rod is slidably connected in the holding block one, a groove is reserved on the inner wall of the support, the head of the linkage rod is slidably connected to the groove in the support, an energy storage component one is fitted outside the linkage rod, a linkage block one is fitted outside the linkage rod, a connecting block is slidably connected to the upper part of the holding block one, a linkage block two is fixedly connected to the lower part of the connecting block, the inclined wall of the linkage block two is slidably connected to the inclined wall of the linkage block one, and a balance block is fitted to the outside of the holding component.

[0007] Preferably, a right-angled block is mounted on the outer wall of the first holding block, a protrusion is mounted on the outer wall of the second holding block, the protrusion being slidably connected to the right-angled block, and a protrusion being mounted on the outer wall of the right-angled block, the protrusion being slidably connected to the support.

[0008] Preferably, an energy storage component two is assembled between the first holding block and the second holding block, the upper part of the energy storage component two is fixedly connected to the lower wall of the first holding block, and the lower part of the energy storage component two is fixedly connected to the upper wall of the second holding block.

[0009] Preferably, a connecting block is fixedly connected to the upper wall of the second holding block, the connecting block is slidably connected to the first holding block, and the upper part of the connecting block extends out of the upper wall of the first holding block.

[0010] Preferably, the head of the linkage rod passes through the right-angle block, the part of the linkage rod extending out of the right-angle block slides with the support, and a rotating ball is screwed onto the head of the linkage rod.

[0011] Preferably, a ring cover is fitted on the outer wall surface of the linkage lever, the energy storage component is fixedly connected to the ring cover, and the part of the energy storage component away from the ring cover is fixedly connected to the inner wall surface of the holding block.

[0012] Preferably, a flange is fitted on the outer wall of the connecting block, the flange is slidably connected to the inner wall of the holding block, an outer cover is slidably connected to the upper part of the connecting block, a connecting piece is fitted on the side wall of the outer cover, and the connecting piece is in contact with the connecting block.

[0013] Preferably, the upper parts of the connecting block and the linking block are assembled on the upper part of the holding block, and the upper wall surface of the linking block is lower than the upper wall surface of the linking block.

[0014] Preferably, the support has a pre-reserved assembly cavity, the balance block slides with the inner wall of the assembly cavity, the balance block has a pre-reserved through-hole, a ring block slides in the assembly cavity, the ring block has a pre-reserved through-hole, two pairs of transmission lines are fixedly connected to the outer wall of the holding block, a transmission plate is assembled in the support, the transmission line contacts the transmission plate, the transmission line is fixedly connected to the side wall of the holding block, and the part of the transmission line away from the holding block is fixedly connected to the balance block.

[0015] The beneficial effects of this invention are:

[0016] 1. Before conducting cell disruption experiments, this invention involves adjusting the outer cover to facilitate the attachment of containers of varying sizes. The containers are placed on container block one, and the container is pressed down on the connecting block, causing container block two, along with the lower part of the elastic cover, to shift downwards. This causes the elastic cover to be pulled and narrowed, creating space between the elastic cover and the receiving opening. Then, the container is pressed down on the connecting block, causing the connecting block to shift downwards along with linkage block two. Through the sliding connection between the inclined walls of linkage block one and linkage block two, the linkage lever is retracted into container block one. The container reaches the lower wall of the receiving opening under the pressure of the container. The energy storage component one is shortened by the pressure, the balance block is pulled upwards, and the container is shifted downwards into the receiving opening.

[0017] 2. In this invention, when the container is removed, the balance block shifts downwards, pulling the container upwards to the upper part of the receiving opening. The energy storage element unfolds, with the head of the linkage lever extending out of the container block and into the support, causing the support to return to its original position. The container plug seals the upper opening of the receiving opening to prevent foreign matter and impurities from accumulating inside, ensuring smooth movement of the container within the support and facilitating the smooth progress of cell disruption experiments. After placing the container on the container, the lower wall of the container first contacts the connecting block. Pressing the connecting block downwards causes it to move upwards, promoting the smooth movement of the container. When the second block is displaced downwards, the length between the first and second holding blocks increases. Here, the second energy storage component expands, and the second holding block pulls the lower part of the elastic cover downwards, thus creating space between the elastic cover and the receiving opening. This provides movable space for the holding component, facilitating its smooth movement up and down within the receiving opening. When the bottle is removed, the holding component displaces upwards back to its original position, separating the bottle from the connecting block. The second energy storage component pulls the second holding block upwards, causing the elastic cover to return to its original position and sealing the space between the holding component and the receiving opening.

[0018] 3. In this invention, the container is placed on the holding component, and the holding component is pushed downwards. The transmission line is dragged to cause the balance block to shift upwards. When the container is removed, the balance block shifts downwards, and the holding component is pulled upwards via the transmission plate and transmission line, which facilitates the smooth lifting and lowering of the holding component. The installation of the balance block enhances the stability of the lifting and lowering of the holding component. The balance block is in contact with the inner wall of the assembly cavity, but does not abut or squeeze against it. During the stage when the container is placed on the holding component, the balance block shifts upwards, and the fluid originally located above the balance block in the assembly cavity will be guided to the lower part of the balance block. Through the installation of the through-hole, the fluid flow rate is limited to prevent the balance block from moving upwards too quickly, which enhances the stability of the downward shift of the container and helps protect the container and the cell fluid to be broken up contained in the container, preventing the cell fluid from spilling out.

[0019] 4. In this invention, the outer wall of the ring block does not adhere to the inner wall of the assembly cavity, leaving space between them to allow fluid to pass through. The installation of the ring block increases the mass of the balance block. During the downward displacement of the balance block, the fluid discharged upward through the first through-hole will exert an upward thrust on the wall of the ring block that does not have a second through-hole, preventing the ring block from blocking the first through-hole. When the balance block is displaced upward, the ring block presses against the balance block, blocking the first through-hole on the balance block. This prevents the fluid in the upper part of the balance block in the assembly cavity from flowing too quickly to the lower part of the balance block, thus protecting the activity of the container and the cell fluid contained in the container, preventing cell fluid from spilling out, and ensuring the smooth progress of the cell disruption experiment.

[0020] Other features and advantages of the invention will be set forth in the following description, and will be apparent in part from the description, or may be learned by practicing the invention. The objects and other advantages of the invention may be realized and obtained by means of the structures particularly pointed out in the description and the drawings. Attached Figure Description

[0021] The accompanying drawings are provided to further illustrate the invention and form part of the specification. They are used together with the embodiments of the invention to explain the invention and do not constitute a limitation thereof. In the drawings:

[0022] Figure 1 This is a structural diagram of the cell disruptor of the present invention;

[0023] Figure 2 This is a structural diagram of the working chamber of the present invention;

[0024] Figure 3 This is a structural diagram of the container of the present invention;

[0025] Figure 4 This is a structural diagram of the support of the present invention;

[0026] Figure 5 This is a structural diagram of the linkage lever of the present invention;

[0027] Figure 6 This is a structural diagram of the right-angled block of the present invention;

[0028] Figure 7 This is a structural diagram of the container block two of the present invention;

[0029] Figure 8 This is a structural diagram of the container block one of the present invention;

[0030] Figure 9 This is a structural diagram of the elastic coating of the present invention;

[0031] Figure 10 This is a structural diagram of the linkage block one of the present invention;

[0032] Figure 11 This is a structural diagram of the second linkage block of the present invention;

[0033] Figure 12 For the present invention Figure 4 M-region structure diagram;

[0034] Figure 13 This is a structural diagram of the balance block of the present invention.

[0035] Reference numerals: 100, Cell disruptor; 101, Working chamber; 102, Ultrasonic probe; 200, Support; 201, Assembly cavity; 202, Receiving port; 203, Ring block; 204, Through-hole two; 300, Transfer line; 301, Transfer plate; 302, Through-hole one; 303, Balance block; 400, Container; 401, Container block one; 500, Container block two; 501, Protrusion one; 502, Protrusion two; 503, Right-angle block; 504, Elastic covering; 505, Energy storage component two; 506, Connecting block; 600, Linkage lever; 601, Energy storage component one; 602, Ring cover; 603, Linkage block one; 604, Rotating ball; 700, Connecting block; 701, Linkage block two; 702, Flange; 703, Outer cover; 704, Connecting piece. Detailed Implementation

[0036] To make the objectives, technical solutions, and advantages of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. The same reference numerals in the drawings represent the same components. It should be noted that the described embodiments are only some, not all, of the embodiments of the present invention. All other embodiments obtained by those skilled in the art based on the described embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0037] Reference Figures 1-13 This invention provides an ultrasonic cell disruptor, comprising a cell disruptor 100, a working chamber 101 within the cell disruptor 100, an ultrasonic probe 102 mounted on the cell disruptor 100, a support 200 mounted in the working chamber 101, the support 200 being mounted on the lower part of the ultrasonic probe 102, a receiving opening 202 pre-formed on the support 200, a container 400 slidably connected to the receiving opening 202, and the container 400 being mounted on the upper part of the receiving opening 202. The container 400 includes a first container block 401, a second container block 500 is mounted on the lower part of the first container block 401, a right-angle block 503 is mounted on the outer wall of the first container block 401, a first protrusion 501 is mounted on the outer wall of the second container block 500, the first protrusion 501 is slidably connected to the right-angle block 503, a second protrusion 502 is mounted on the outer wall of the right-angle block 503, the second protrusion 502 is slidably connected to the support 200, which facilitates the smooth lifting and lowering of the container 400 in the receiving opening 202.

[0038] Elastic coverings 504 are fitted on the outer walls of holding block 1 401 and holding block 2 500. The elastic coverings 504 are in close contact with the inner wall of the receiving opening 202. A linkage rod 600 is slidably connected in holding block 1 401. The head of the linkage rod 600 passes through the right-angle block 503. The part of the linkage rod 600 that extends out of the right-angle block 503 is slidably connected to the support 200. A groove is reserved on the inner wall of the support 200. The head of the linkage rod 600 is slidably connected to the groove in the support 200. A rotating ball 604 is screwed onto the head of the linkage rod 600.

[0039] An energy storage component 601 is mounted on the outside of the linkage lever 600. A cover 602 is mounted on the outer wall of the linkage lever 600. The energy storage component 601 is fixedly connected to the cover 602. The part of the energy storage component 601 away from the cover 602 is fixedly connected to the inner wall of the holding block 401. A linkage block 603 is fixedly connected to the part of the linkage lever 600 away from the rotating ball 604. A connecting block 700 is slidably connected to the upper part of the holding block 401. A flange 702 is mounted on the outer wall of the connecting block 701. The flange 702 is connected to the holding block 401. The inner wall is slidably connected, and the upper part of the connecting block 700 is slidably connected to the outer cover 703. The side wall of the outer cover 703 is equipped with a connecting piece 704. The connecting piece 704 is in contact with the connecting block 700, which helps to prevent the outer cover 703 from sliding on the connecting block 700. The lower part of the connecting block 700 is fixedly connected to the second linkage block 701. The inclined wall of the second linkage block 701 is slidably connected to the inclined wall of the first linkage block 603. The outside of the container 400 is equipped with a balance block 303. Moving the outer cover 703 helps to fit containers of different sizes.

[0040] Place the container bottle on the first container block 401, and press the connecting block 506 downwards with the container bottle facing down. This causes the second container block 500, along with the lower part of the elastic covering 504, to shift downwards, causing the elastic covering 504 to be pulled and narrowed, thus creating space between the elastic covering 504 and the receiving opening 202. Then, press the connecting block 700 downwards with the container bottle facing down. The connecting block 700 causes the second linkage block 701 to shift downwards. Through the sliding contact between the first linkage block 603 and the inclined wall of the second linkage block 701, the linkage lever 600 is retracted into the first container block 401. The container 400 reaches the lower wall of the receiving opening 202 under the pressure of the container bottle. The first energy storage component 601 is shortened by the pressure, and the balance block 303 is pulled upwards. The container bottle shifts downwards and is positioned in the receiving opening 202.

[0041] When the container is removed, the balance block 303 shifts downwards, pulling the container 400 upwards to the upper part of the receiving opening 202. The energy storage component 601 extends out of the container block 401 with the head of the linkage lever 600 and extends into the support 200, causing the support 200 to return to its original position. The container 400 seals the upper opening of the receiving opening 202 to prevent foreign matter and impurities from accumulating in the receiving opening 202, ensuring that the container moves smoothly up and down in the support 200, which is conducive to the smooth progress of the cell disruption experiment.

[0042] An energy storage component 2 505 is assembled between holding block 1 401 and holding block 2 500. The upper part of the energy storage component 2 505 is fixedly connected to the lower wall of holding block 1 401, and the lower part of the energy storage component 2 505 is fixedly connected to the upper wall of holding block 2 500.

[0043] A connecting block 506 is fixedly connected to the upper wall of the second holding block 500. The connecting block 506 is slidably connected to the first holding block 401. The upper part of the connecting block 506 extends out of the upper wall of the first holding block 401. The upper parts of the connecting block 506 and the connecting block 700 are assembled on the upper part of the first holding block 401. The upper wall of the connecting block 700 is lower than the upper wall of the connecting block 506.

[0044] After the container bottle is placed on the holding component 400, the lower wall of the container bottle first adheres to the connecting block 506. Pushing the connecting block 506 downwards causes the second holding block 500 to shift downwards, increasing the length between the first holding block 401 and the second holding block 500. Here, the second energy storage component 505 expands, and the second holding block 500 pulls the lower part of the elastic covering 504 downwards, thereby causing the elastic covering 504 and the receiving opening 20 to... The space between the two compartments provides room for the container 400 to move freely, facilitating its smooth movement within the receiving opening 202. When the container is removed, the container 400 moves upwards to return to its original position, separating the container and the connecting block 506. The energy storage component 505 pulls the container block 500 upwards, causing the elastic covering 504 to return to its original position and seal the space between the container 400 and the receiving opening 202.

[0045] The support 200 has a pre-reserved assembly cavity 201. The balance block 303 slides in contact with the inner wall of the assembly cavity 201. Two pairs of transmission lines 300 are fixedly connected to the outer wall of the holding block 401. The support 200 is equipped with a transmission plate 301. The transmission line 300 contacts the transmission plate 301. The transmission line 300 is fixedly connected to the side wall of the holding block 401. The part of the transmission line 300 away from the holding block 401 is fixedly connected to the balance block 303. The balance block 303 is in the shape of a ring.

[0046] The container bottle is placed on the container 400. Pressing the container 400 downwards and dragging the transmission line 300 causes the balance block 303 to shift upwards. When the container bottle is removed, the balance block 303 shifts downwards and pulls the container 400 upwards via the transmission plate 301 and the transmission line 300, which facilitates the smooth lifting and lowering of the container 400.

[0047] The balance block 303 has a pre-reserved through-hole 302. The installation of the balance block 303 helps to enhance the stability of the lifting and lowering of the container 400. The balance block 303 is attached to the inner wall of the assembly cavity 201, but the balance block 303 does not abut against the inner wall of the assembly cavity 201. When the container is placed on the container 400, the balance block 303 moves upward. The fluid originally located above the balance block 303 in the assembly cavity 201 will be guided to the lower part of the balance block 303. Through the installation of the through-hole 302, the fluid flow rate is limited to prevent the balance block 303 from moving upward too fast. This helps to enhance the stability of the container moving downward and protects the container and the cell fluid to be broken up contained in the container to prevent the cell fluid from spilling out.

[0048] A ring block 203 is slidably mounted in the assembly cavity 201. A second through-hole 204 is pre-reserved in the ring block 203. The outer wall of the ring block 203 does not contact the inner wall of the assembly cavity 201, leaving space between them to allow fluid passage. The installation of the ring block 203 helps to increase the mass of the balance block 303. During the downward displacement phase of the balance block 303, the fluid discharged upward through the first through-hole 302 will exert pressure on the wall surface of the ring block 203 where the second through-hole 204 is not pre-reserved. An upward thrust is applied to prevent the ring block 203 from blocking the through-hole 302. When the balance block 303 shifts upward, the ring block 203 presses against the balance block 303, blocking the through-hole 302 on the balance block 303. This prevents the fluid in the assembly cavity 201 located above the balance block 303 from flowing too quickly to the lower part of the balance block 303, which helps protect the activity of the container and the cell fluid contained in the container, prevents the cell fluid from spilling out, and ensures the smooth progress of the cell disruption experiment.

[0049] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the present invention as claimed. The scope of protection of this invention is defined by the appended claims and their equivalents.

Claims

1. An ultrasonic cell disruptor, comprising a cell disruptor (100), a working chamber (101) is reserved in the cell disruptor (100), and an ultrasonic probe (102) is assembled on the cell disruptor (100), characterized in that, The work bin (101) is provided with a support (200), the support (200) is arranged at the lower part of the ultrasonic probe (102), the support (200) is provided with a containing opening (202), the containing opening (202) is slidably connected with a containing piece (400), the containing piece (400) comprises a containing block one (401), the lower part of the containing block one (401) is provided with a containing block two (500), the outer wall surface of the containing block one (401) and the containing block two (500) is provided with an elastic covering body (504), the elastic covering body (504) is tightly attached to the inner wall surface of the containing opening (202), the containing block one (401) is slidably connected with a linkage lever (600), the inner wall surface of the support (200) is provided with a groove, the head of the linkage lever (600) is slidably connected with the groove in the support (200), the linkage lever (600) is externally provided with an energy storage piece one (601), the linkage lever (600) is externally provided with a linkage block one (603), the upper part of the containing block one (401) is slidably connected with a connecting block (700), the lower part of the connecting block (700) is fixedly connected with a linkage block two (701), the inclined wall surface of the linkage block two (701) is slidably connected with the inclined wall surface of the linkage block one (603), the outer part of the containing piece (400) is provided with a balance block (303), the support (200) is provided with an assembly cavity (201), the balance block (303) is slidably connected with the inner wall surface of the assembly cavity (201), the balance block (303) is provided with a through opening one (302), the assembly cavity (201) is slidably connected with a ring block (203), the ring block (203) is provided with a through opening two (204), the outer wall surface of the containing block one (401) is fixedly connected with two pairs of transmission lines (300), the support (200) is provided with a transmission disc (301), the transmission lines (300) are in contact with the transmission disc (301), the transmission lines (300) are fixedly connected with the edge wall surface of the containing block one (401), the part of the transmission lines (300) away from the containing block one (401) is fixedly connected with the balance block (303).

2. An ultrasonic cell disruptor according to claim 1, wherein The outer wall surface of the containing block one (401) is provided with a right-angle block (503), the outer wall surface of the containing block two (500) is provided with a convex block one (501), the convex block one (501) is slidably connected with the right-angle block (503), the outer wall surface of the right-angle block (503) is provided with a convex block two (502), the convex block two (502) is slidably connected with the support (200).

3. An ultrasonic cell disruptor according to claim 1, wherein The containing block one (401) and the containing block two (500) are provided with an energy storage piece two (505), the upper part of the energy storage piece two (505) is fixedly connected with the lower wall surface of the containing block one (401), the lower part of the energy storage piece two (505) is fixedly connected with the upper wall surface of the containing block two (500).

4. An ultrasonic cell disruptor according to claim 1, wherein The upper wall surface of the second containing block (500) is fixedly connected with a connecting block (506), the connecting block (506) is slidably connected with the first containing block (401), and the upper part of the connecting block (506) extends out of the upper wall surface of the first containing block (401).

5. An ultrasonic cell disruptor according to claim 2, wherein The head of the linkage lever (600) penetrates through the right-angle block (503), the part of the linkage lever (600) extending out of the right-angle block (503) is slidably connected with the support (200), and the head of the linkage lever (600) is rotatably connected with a rotating ball (604).

6. An ultrasonic cell disruptor according to claim 1, wherein The outer wall surface of the linkage lever (600) is provided with a ring cover (602), the energy storage member (601) is fixedly connected with the ring cover (602), and the part of the energy storage member (601) away from the ring cover (602) is fixedly connected with the inner wall surface of the first containing block (401).

7. An ultrasonic cell disruptor according to claim 1, wherein The outer wall surface of the connecting block (700) is provided with a flange (702), the flange (702) is slidably connected with the inner wall surface of the first containing block (401), the upper part of the connecting block (700) is slidably connected with an outer cover (703), the side wall surface of the outer cover (703) is provided with a connecting piece (704), and the connecting piece (704) is attached to the connecting block (700).

8. An ultrasonic cell disruptor according to claim 4, wherein The upper parts of the connecting block (506) and the connecting block (700) are arranged on the upper part of the first containing block (401), and the upper wall surface of the connecting block (700) is lower than the upper wall surface of the connecting block (506).

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