Incubator for cell culture

Abstract

The invention discloses a culture box for cell culture, and relates to the technical field of cell culture, the culture box comprises a culture box, an observation window, a box cover, a support frame, a culture dish, a placing plate and an oscillation unit, the oscillation unit comprises a driving assembly, a clamping assembly and an adjusting assembly; a closed-loop wave groove is formed in the inner side wall of the incubator and is used for guiding the placing plate to vibrate up and down in a wave shape; the placing plate is of a disc structure, is fixed above the driving assembly through a spherical hinge, is positioned in the culture box and is used for placing a culture dish; a guide rod is fixed on the placing plate, is of an elastic telescopic structure and is used for being matched with the wave groove, guiding the placing plate to move along a preset track and carrying out three-dimensional autorotation oscillation under multiple cooperation of autorotation driven by the driving assembly and angle adjustment of the adjusting assembly; the technical effects that oscillation modes are diversified, cell deposition and uneven distribution are avoided, the cell metabolism consistency is improved, and the cell survival rate is improved can be achieved.

Description

Technical Field

[0001] This invention relates to the field of cell culture technology, and more particularly to an incubator for cell culture. Background Technology

[0002] Cell incubators are key equipment in cell culture experiments, typically providing stable and suitable environmental conditions for cell growth, including parameters such as temperature, humidity, and gas composition (e.g., carbon dioxide concentration). Stable conditions are the foundation for normal cell growth, proliferation, and metabolism. Fluctuations in any parameter can affect the physiological state of cells, leading to inaccurate experimental results or even experimental failure. The performance of the incubator is directly related to the reliability and reproducibility of experiments, making it an indispensable tool for advancing research in related fields.

[0003] In cell culture, existing cell culture bags typically employ a two-dimensional elliptical oscillation trajectory, moving in all directions. This oscillation pattern is singular, and the continuous unidirectional oscillation creates a unidirectional driving force within the bag, leading to nutrient gradients. Cells at the center of the vortex are thrown towards the bag wall and deposit, causing abnormally high cell density at the edges. This results in localized overload, failing to meet the requirements for uniform culture of large-scale suspension cells and ultimately leading to cell clumping. Simultaneously, nutrients circulate only unidirectionally along the vortex path, resulting in a single flow path. This can cause edge-deposited cells to suffer from hypoxia or nutrient deficiency, hindering proliferation or even causing apoptosis, disrupting cellular metabolic consistency. Furthermore, continuous repetitive shear stress, through accumulated mechanical stress, can damage cell membrane integrity, reduce cell viability, and consequently affect overall culture efficiency. Summary of the Invention

[0004] This application provides an incubator for cell culture, which solves the technical problems of single oscillation mode, uneven cell deposition and distribution, reduced cell metabolic consistency and cell survival rate in the prior art, and achieves the technical effects of multiple oscillation modes, avoiding uneven cell deposition and distribution, improving cell metabolic consistency and cell survival rate.

[0005] This application provides a cell culture incubator, including an incubator, an observation window, a lid, a support frame, a culture dish, a placement plate, and an oscillation unit, wherein the oscillation unit includes a driving component, a clamping component, and an adjustment component; The inner wall of the incubator is provided with a closed-loop wave groove to guide the placement plate to vibrate up and down in a wave-like manner. The placement plate is a disc structure, fixed above the drive assembly and located inside the incubator by a ball joint, and is used to place the culture dish; a guide rod is fixed on the placement plate, the guide rod is an elastic telescopic structure, used to cooperate with the wave groove to guide the placement plate to move along a predetermined trajectory, and to perform three-dimensional rotation oscillation under the multiple cooperation of the drive assembly driving rotation and the adjustment assembly adjusting angle.

[0006] Furthermore, the drive assembly includes a motor, a drive column, and a connecting rod; The motor is fixed to the bottom of the incubator, and its output end is connected to the drive column through a coupling to drive the drive column to rotate. There are two sets of connecting rods, one end of which is fixed to the left and right sides of the drive column, and the other end is fixed to the bottom of the placement plate to transmit power to the placement plate.

[0007] Furthermore, the connecting rod includes connecting rod one and connecting rod two; One end of the connecting rod is connected to the bottom of the placement plate, and the other end is hinged to the connecting rod. The connecting rod is slidably connected in the drive column. The rotation of the drive column drives the connecting rod to rotate, thereby driving the placement plate to rotate and move up and down.

[0008] Furthermore, the clamping assembly is provided in multiple sets, evenly arranged along the circumference of the placement plate, for clamping and fixing the culture dish, including a fixing plate, clamping plate, pull rod and spring; The fixing plate is fixed on the placement plate; the clamping plate is slidably connected to the inside of the fixing plate by a pull rod, and the clamping plate and the fixing plate are connected by a spring, which is used to clamp and fix the culture dish circumferentially by the elastic force of the spring.

[0009] Furthermore, the hinge seat at the top of the connecting rod is connected to the bottom of the placement plate via a ball joint.

[0010] Furthermore, the adjustment assembly is provided in two sets, which are fixed to the front and rear sides of the drive column respectively, for adjusting the tilt angle of the placement plate, including rollers and pull ropes; The roller is fixed to the drive column and is driven to rotate by the motor inside it; one end of the pull rope is wound around the roller and the other end is connected to the placement plate. The roller winds up the pull rope, thereby adjusting the tilt angle of the placement plate to achieve three-dimensional oscillation.

[0011] Furthermore, the inner wall of the incubator is embedded with multiple protrusions and concave blocks. The protrusions are upward-convex arc-shaped structures, and the concave blocks are U-shaped structures. The two are located below the crests and troughs of the wave groove, respectively, and correspond one-to-one with them.

[0012] Furthermore, both the protrusions and concave blocks are hollow bladder structures, and their bottoms are made of hard material. Both are divided into three spaces, namely air chamber one, air chamber two, air chamber three and chamber one, chamber two, chamber three.

[0013] Furthermore, air chamber one, air chamber two, and air chamber three are connected to an external air pump through independent pipes, which are used to change the volume of the protrusions and concave blocks by inflating or deflating, thereby changing the curvature of the wave crests and troughs of the wave groove, and thus changing the degree of oscillation during the shaking.

[0014] Furthermore, the longitudinal expansion heights of air chamber 1, air chamber 2, air chamber 3, chamber 1, chamber 2, and chamber 3 are all the same in the fully expanded state; when the curvature at the trough is the largest, there is no gas in chamber 3 and chamber 2 and they are in a close fit; when the curvature at the crest is the largest, air chamber 1, air chamber 2, and air chamber 3 are all in a fully expanded state.

[0015] One or more technical solutions provided in this application have at least the following technical effects or advantages: By designing a unique wave-shaped groove, the liquid in the cell culture dish generates a more complex multidirectional flow. Driven by the drive component, the placement plate and culture dish move along a specific trajectory, performing three-dimensional oscillation. This allows for more comprehensive mixing of cells and culture medium, promoting uniform distribution of nutrients and effective removal of metabolic waste. Through the coordinated work of the vibration component, drive component, and regulation component, comprehensive dynamic control of the cell culture environment is achieved. This effectively solves the technical problems of existing technologies, such as single oscillation mode, uneven cell deposition and distribution, reduced cell metabolic consistency, and reduced cell survival rate. It achieves the technical effects of multiple oscillation modes, avoidance of uneven cell deposition and distribution, improved cell metabolic consistency, and increased cell survival rate. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the overall structure of a cell culture incubator according to the present invention.

[0017] Figure 2 This is a partial three-dimensional cross-sectional view of an incubator for cell culture according to the present invention.

[0018] Figure 3 This is a longitudinal full sectional view of the dust removal component of a cell culture incubator according to the present invention.

[0019] Figure 4 This is a three-dimensional structural diagram of the driving component and clamping component of a cell culture incubator according to the present invention.

[0020] Figure 5 This is a schematic diagram showing the state of the placement plate of a cell culture incubator of the present invention when it is rotated to the highest point of the spiral groove and tilted longitudinally.

[0021] Figure 6This is a schematic diagram of the state of the placement plate of a cell culture incubator according to the present invention when it is rotated to the highest point of the spiral groove and swings back and forth under the action of the adjustment component.

[0022] Figure 7 This is a three-dimensional cross-sectional view of the culture chamber and spiral groove of a cell culture incubator according to the present invention.

[0023] Figure 8 This is a cross-sectional view of the protrusion of a cell culture incubator according to the present invention.

[0024] Figure 9 This is a cross-sectional view of the recessed block of a cell culture incubator according to the present invention.

[0025] In the diagram: 100, incubator; 101, observation window; 102, lid; 103, support frame; 104, petri dish; 105, wave groove; 110, placement plate; 111, guide rod; 120, drive assembly; 121, motor one; 122, drive column; 130, clamping assembly; 131, fixing plate; 132, clamping plate; 133, pull rod; 134, spring; 140, connecting rod; 141, connecting rod one; 142, connecting rod two; 150, adjusting assembly; 151, roller; 152, pull rope; 160, protrusion; 161, air chamber one; 162, air chamber two; 163, air chamber three; 170, concave block; 171, chamber one; 172, chamber two; 173, chamber three. Detailed Implementation

[0026] To facilitate understanding of the present invention, a more complete description of this application will be given below with reference to the accompanying drawings, which illustrate preferred embodiments of the invention. However, the invention can be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided to enable a more thorough and complete understanding of the disclosure of the present invention.

[0027] It should be noted that the terms "vertical," "horizontal," "up," "down," "left," "right," and similar expressions used in this article are for illustrative purposes only and do not represent the only possible implementation.

[0028] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains; the terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to limit the invention; the term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0029] Please see Figure 1This is a schematic diagram of the overall structure of a cell culture incubator according to the present invention. The cell culture incubator 100 of this application, by setting a unique wave groove 105, enables the liquid in the cell culture dish 104 to generate more complex multidirectional flow. Under the drive of the drive component 120, the placement plate 110 and the culture dish 104 can move along a specific trajectory, performing three-dimensional oscillation motion. This allows for more comprehensive mixing of cells and culture medium, promoting uniform distribution of nutrients and effective removal of metabolic waste. Through the coordinated work of the vibration component, drive component 120, and adjustment component 150, comprehensive dynamic control of the cell culture environment is achieved. This results in the technical effects of multiple oscillation modes, avoidance of cell deposition and uneven distribution, improved cell metabolic consistency, and increased cell survival rate.

[0030] Example 1: As Figures 1 to 6 As shown, this application discloses a cell culture incubator, which includes an incubator 100, an observation window 101, a lid 102, a support frame 103, a culture dish 104, a placement plate 110, and an oscillation unit. The oscillation unit includes a drive assembly 120, a clamping assembly 130, and an adjustment assembly 150. The inner wall of the incubator 100 is provided with a closed-loop wave groove 105, which is used to guide the placement plate 110 to vibrate up and down in a wave-like manner. The placement plate 110 is a disc structure, which is fixed above the drive assembly 120 and located inside the incubator 100 by a ball joint, and is used to place the culture dish 104. A guide rod 111 is fixed on the placement plate 110. The guide rod 111 is an elastic telescopic structure, which is used to cooperate with the wave groove 105 to guide the placement plate 110 to move along a predetermined trajectory, and to perform three-dimensional rotation oscillation under the multiple cooperation of the drive assembly 120 driving the rotation and the adjustment assembly 150 adjusting the angle.

[0031] The drive assembly 120 includes a motor 121, a drive column 122, and a connecting rod 140. The motor 121 is fixed at the bottom of the incubator 100, and its output end is connected to the drive column 122 through a coupling to drive the drive column 122 to rotate; the connecting rod 140 is provided in two sets, one end of which is fixed to the left and right sides of the drive column 122 respectively, and the other end is fixed to the bottom of the placement plate 110 to transmit power to the placement plate 110.

[0032] The connecting rod 140 includes a first connecting rod 141 and a second connecting rod 142; One end of the first connecting rod 141 is connected to the bottom of the placement plate 110, and the other end is hinged to the second connecting rod 142; the second connecting rod 142 is slidably connected in the drive column 122, and the rotation of the drive column 122 drives the connecting rod 140 to rotate, thereby driving the placement plate 110 to rotate and move up and down.

[0033] The clamping assembly 130 is provided in multiple sets and is evenly arranged around the circumference of the placement plate 110 for clamping and fixing the culture dish 104, including a fixing plate 131, a clamping plate 132, a pull rod 133 and a spring 134. The fixing plate 131 is fixed on the placement plate 110; the clamping plate 132 is slidably connected to the inner side of the fixing plate 131 by the pull rod 133, and the clamping plate 132 and the fixing plate 131 are connected by a spring 134, which is used to clamp and fix the culture dish 104 in the circumferential direction by the elastic force of the spring 134.

[0034] The hinge seat at the top of the connecting rod 141 is connected to the bottom of the placement plate 110 by a ball joint.

[0035] The adjustment assembly 150 is provided in two sets, which are respectively fixed on the front and rear sides of the drive column 122 and are used to adjust the tilt angle of the placement plate 110, including the roller 151 and the pull rope 152. The roller 151 is fixed on the drive column 122 and is driven to rotate by the internal motor. One end of the pull rope 152 is wound around the roller 151, and the other end is connected to the placement plate 110. The pull rope 152 is wound up by the roller 151, thereby adjusting the tilt angle of the placement plate 110 to achieve three-dimensional oscillation.

[0036] By setting a unique wave groove 105 and employing a wave-shaped three-dimensional oscillation trajectory and multi-directional compound motion (rotation + up-and-down wave movement), the liquid in the cell culture dish 104 generates a more complex multi-directional flow, rather than the unidirectional vortex under the traditional elliptical trajectory. This effectively disrupts the central low-pressure zone, preventing nutrients from forming a unidirectional circulation in the culture medium. The multi-directional flow promotes thorough mixing of the culture medium in three-dimensional space, allowing nutrients and metabolic waste to quickly diffuse throughout the entire culture system, thereby achieving uniform distribution of nutrients throughout the bag and eliminating nutrient gradients. At the same time, the fluid shear force generated by the wave-shaped three-dimensional oscillation trajectory is asymmetrical and its direction is constantly changing, which keeps the cells in a continuous suspension state, effectively preventing cells from depositing at the bottom or edge of the culture dish 104 due to gravity. This is effectively applied to the large-scale culture of suspension cells, preventing cell deposition, ensuring uniform distribution of cells in the culture medium, and improving cell culture efficiency.

[0037] By changing the connection method between connecting rod 141 and placement plate 110 (to a ball joint) and setting two sets of adjustment components 150, the tilt angle of culture dish 104 is adjusted with the line connecting the two sets of connecting rods 141 and the bottom hinge position of placement plate 110 as the axis. At the same time, drive component 120 drives placement plate 110 to rotate, and wave groove 105 guides placement plate 110 to oscillate up and down. The coordinated action of multiple components ultimately realizes three-dimensional oscillation of culture dish 104. Compared with two-dimensional elliptical oscillation, three-dimensional oscillation can more thoroughly mix the culture medium, allowing cells to be subjected to more uniform hydrodynamic action in three-dimensional space, further optimizing the cell culture environment.

[0038] Furthermore, guided by the wave-shaped three-dimensional oscillation trajectory, the shear force is applied intermittently and in multiple directions through the ball joint drive mechanism combined with the wave track. This effectively avoids the cell membrane being subjected to continuous, unidirectional mechanical stress impact, reduces the probability of cell membrane damage, thereby reducing cell rupture and death, and improving cell survival rate.

[0039] In actual operation, the steps of this embodiment are as follows: First, the culture dish 104 is placed on the placement plate 110, and the pull rod 133 is manually pulled to move the clamping plate 132, thereby clamping and fixing the culture dish 104 circumferentially; then, the motor 121 is started, driving the drive column 122 to rotate, which in turn drives the connecting rod 140 to make the placement plate 110 start to rotate. Under the guidance of the guide rod 111 and the wave groove 105, the placement plate 110 moves up and down along the wave groove 105, realizing a self-rotating up and down oscillating vibration with the drive column 122 as the rotation axis; finally, the internal motors of the two rollers 151 are started, respectively driving the corresponding rollers 151 to wind up and release the pull rope 152, adjusting the tilt angle of the placement plate 110, and realizing three-dimensional oscillation during the self-rotating up and down oscillation of the placement plate 110, which improves the oscillation effect of the cells in the cell culture dish 104, ensures that the cells can be evenly located in the cell culture dish 104, avoids cell deposition and uneven distribution, and improves the cell culture effect.

[0040] The technical solutions described in the embodiments of this application have at least the following technical effects or advantages: By designing a wave-shaped three-dimensional oscillation trajectory, it is possible to break unidirectional vortices, eliminate nutrient gradients, prevent cell deposition, reduce the probability of cell membrane damage, and achieve three-dimensional oscillation, thereby improving cell culture efficiency and cell quality. This effectively solves the technical problems of existing technologies, such as single oscillation modes, uneven cell deposition and distribution, reduced cell metabolic consistency, and decreased cell viability. It achieves the technical effects of multiple oscillation modes, avoidance of uneven cell deposition and distribution, improved cell metabolic consistency, and increased cell viability.

[0041] Example 2: To improve the oscillation effect, meet the culture requirements of different cells, and enhance the flexibility of the device, this application proposes the following technical solution to address the aforementioned technical problems: like Figures 5 to 9 As shown, the inner wall of the incubator 100 is embedded with a plurality of protrusions 160 and concave blocks 170. The protrusions 160 are upward-convex arc-shaped structures, and the concave blocks 170 are concave-shaped structures. The two are located below the crests and troughs of the wave groove 105, respectively, and correspond to each other.

[0042] Both the protrusion 160 and the concave block 170 are hollow bladder structures, and their bottoms are made of hard material. Both are divided into three spaces, namely air chamber 161, air chamber 2 162, air chamber 3 163 and chamber 171, chamber 2 172, chamber 3 173.

[0043] The air chambers 161, 162, and 163 are connected to the external air pumps via independent pipes, and are used to change the volume of the protrusions 160 and concave blocks 170 by inflating or deflating, thereby changing the curvature of the wave crests and troughs of the wave groove 105, and thus changing the degree of oscillation during the shaking.

[0044] When fully expanded, the longitudinal expansion heights of air chamber 161, air chamber 2 162, air chamber 3 163, chamber 171, chamber 2 172, and chamber 3 173 are all the same. When the curvature at the trough is the largest, there is no gas in chamber 3 173 and chamber 2 172 and they are in a close-fitting state. When the curvature at the crest is the largest, air chamber 161, air chamber 2 162, and air chamber 3 163 are all in a fully expanded state.

[0045] Since different types of cells (such as stem cells, tumor cells, and nerve cells) have different sensitivities and requirements to mechanical stimulation, the curvature of the wave crests and troughs of the wave groove 105 can be dynamically controlled by inflating and deflating, thereby adjusting the steepness or gentleness of the movement trajectory of the wave groove 105 and precisely controlling the degree of oscillation. This not only simulates the mechanical conditions in different tissue environments and meets the needs of cells with different culture requirements, but also finds the most suitable oscillation conditions for cell growth. This is beneficial for optimizing subsequent cell culture processes, improving cell culture efficiency and product quality, and meeting diverse culture needs.

[0046] When the curvature of the wave crests and troughs of the wave groove 105 increases, the movement trajectory of the wave groove 105 becomes steeper, the oscillation amplitude and speed of the culture dish 104 increase, and the liquid flow becomes more vigorous. This helps to promote full contact between cells and the culture medium, break the concentration gradient, and make nutrients and oxygen more evenly distributed. Vigorous flow can quickly dilute and remove waste products generated by cell metabolism, avoiding the accumulation of toxins. When the curvature decreases, the movement trajectory of the culture dish 104 becomes smoother, and the oscillation is gentler. This is suitable for the culture of shear-sensitive cells (such as neurons and pancreatic islet cells) and can avoid damage caused by excessive oscillation.

[0047] The simulated scenario in this embodiment is as follows: The initial waveform of wave groove 105 can be approximated as a sine function. ,in, This represents the amplitude. Initially, the peaks and troughs have the same amplitude but opposite directions. The initial amplitude of the peak is set. The initial amplitude of the trough (considering direction) To facilitate comparison of changes in curvature, the comparison is primarily based on the absolute value of the amplitude, i.e. , Set the amplitude for each inflation or deflation operation of the air chamber or cavity. absolute change .

[0048] The following two adjustment states are examples: (1) When the curvature at the crest increases, the air chamber 162 and the air chamber 163 are filled with air in sequence. At this time, the amplitude of the sine function at the crest increases. The absolute value increases; Initial state: The waveform is crest curvature corresponds ; After inflating air chamber 2.162: the amplitude increases to The waveform becomes crest curvature corresponds ; After inflating the air chamber 3163 again: the amplitude further increases to The waveform becomes crest curvature corresponds As the amplitude gradually increases, the amplitude at the peak shows a progressively increasing trend.

[0049] (2) When the curvature at the trough increases, the air extraction operation is performed on chamber 3 173 and chamber 2 172 in sequence, so that the absolute value of the amplitude of the sine function at the trough increases (the absolute value of the trough increases). In the initial state, chambers 171, 172, and 173 are all fully inflated. At this time, the amplitude at the trough is... The waveform is trough curvature corresponds ; After evacuating chamber 3173: the amplitude increases to The waveform becomes trough curvature corresponds ; After evacuating chamber 2 (172), the amplitude further increases to The waveform becomes trough curvature corresponds As the amplitude gradually increases, the amplitude at the trough shows a progressively increasing trend.

[0050] In this embodiment, by adjusting the size of the protrusion 160, the numerical value of the wave crest can be changed, that is, the amplitude A of the sine function can be changed. In the initial state, the first air chamber 161 is fully inflated, while the second air chamber 162 and the third air chamber 163 are empty and in a close fit. When adjusting the size of the wave crest of the wave groove 105, it is only necessary to inflate the second air chamber 162 and the third air chamber 163 in sequence, so that the amplitude A of the corresponding sine function increases, thereby increasing the curvature at the wave crest of the wave groove 105 and making its waveform more curved. Conversely, it is only necessary to evacuate the third air chamber 163 and the second air chamber 162 in sequence, so that the curvature at the wave crest of the wave groove 105 decreases and its waveform becomes smoother.

[0051] By adjusting the size of the concave block 170, the absolute value of the trough can be changed, that is, the amplitude A of the sine function can be changed. In the initial state, chambers 171, 172, and 173 are all fully inflated, with the curvature and bending degree being the smallest, and the waveform being the smoothest. To increase the absolute value of the trough of the wave groove 105, it is only necessary to perform air extraction operations on chambers 173 and 172 in sequence, so that the amplitude A of the corresponding sine function increases, thereby increasing the curvature at the trough of the wave groove 105 and making its waveform more curved. When the curvature at the trough is the largest, there is no gas in chambers 173 and 172 and they are in a close fit.

[0052] In the initial stage, a gentle trajectory (low curvature) can promote cell attachment, while switching to a steep trajectory (high curvature) in the later stage can enhance differentiation stimulation. During oscillation, the curvature can be quantitatively adjusted by gas filling and gas emptying operations to ensure that the oscillation trajectory is consistent in each experiment, reduce human error, and improve culture efficiency.

[0053] Appropriate oscillation helps cells adhere and grow evenly at the bottom of the culture dish 104, preventing cell aggregation and clumping, forming a healthy monolayer of cells, improving the uniformity and efficiency of cell culture, and by optimizing the flow of culture medium and shear force distribution, it helps reduce differences between cells, making the cell population more consistent in terms of growth rate, metabolic activity, etc., thus improving the quality of cell products.

[0054] The above description is merely a preferred embodiment of the present invention and is not intended to limit the invention. For those skilled in the art, the present invention can have various modifications and variations. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A cell culture incubator (100), comprising an incubator (100), an observation window (101), a lid (102), a support frame (103), a culture dish (104), a placement plate (110), and an oscillation unit, characterized in that, The oscillation unit includes a driving component (120), a clamping component (130), and an adjustment component (150). The inner wall of the incubator (100) is provided with a closed-loop wave groove, which is used to guide the placement plate (110) to vibrate up and down in a wave shape; The placement plate (110) is a disc structure, which is fixed above the drive assembly (120) and located inside the incubator (100) by ball joint, and is used to place the culture dish (104); a guide rod (111) is fixed on the placement plate (110), which is an elastic telescopic structure, used to cooperate with the wave groove to guide the placement plate (110) to move along a predetermined trajectory, and to perform three-dimensional rotation oscillation under the multiple cooperation of the drive assembly (120) driving rotation and the adjustment assembly (150) adjusting angle.

2. The cell culture incubator (100) as described in claim 1, characterized in that, The drive assembly (120) includes a motor (121), a drive column (122), and a connecting rod (140). The motor (121) is fixed at the bottom of the incubator (100), and its output end is connected to the drive column (122) through a coupling to drive the drive column (122) to rotate; the connecting rod (140) is provided in two sets, one end of which is fixed to the left and right sides of the drive column (122) respectively, and the other end is fixed to the bottom of the placement plate (110) to transmit power to the placement plate (110).

3. The cell culture incubator (100) as described in claim 2, characterized in that, The connecting rod (140) includes connecting rod one (141) and connecting rod two (142). One end of the first connecting rod (141) is connected to the bottom of the placement plate (110), and the other end is hinged to the second connecting rod (142). The second connecting rod (142) is slidably connected in the drive column (122). The rotation of the drive column (122) drives the connecting rod (140) to rotate, thereby driving the placement plate (110) to rotate and move up and down.

4. The cell culture incubator (100) as described in claim 1, characterized in that, The clamping assembly (130) is provided in multiple sets and is evenly arranged around the circumference of the placement plate (110) for clamping and fixing the culture dish (104), including a fixing plate (131), a clamping plate (132), a pull rod (133) and a spring (134). The fixing plate (131) is fixed on the placement plate (110); the clamping plate (132) is slidably connected to the inside of the fixing plate (131) by the pull rod (133), and the clamping plate (132) and the fixing plate (131) are connected by a spring (134) for circumferentially clamping and fixing the culture dish (104) by the elastic force of the spring (134).

5. The cell culture incubator (100) as described in claim 3, characterized in that, The hinge seat at the top of the connecting rod (141) is connected to the bottom of the placement plate (110) by a ball joint.

6. The cell culture incubator (100) as described in claim 5, characterized in that, The adjustment assembly (150) is provided in two sets, which are fixed on the front and rear sides of the drive column (122) respectively, for adjusting the tilt angle of the placement plate (110), including roller (151) and pull rope (152). The roller (151) is fixed on the drive column (122) and driven to rotate by the internal motor; one end of the pull rope (152) is wrapped around the roller (151) and the other end is connected to the placement plate (110). The pull rope (152) is wound up by the roller (151), thereby adjusting the tilt angle of the placement plate (110) to achieve three-dimensional oscillation.

7. The cell culture incubator (100) as described in claim 1, characterized in that, The inner wall of the incubator (100) is embedded with a number of protrusions (160) and concave blocks (170). The protrusions (160) are upward-convex arc-shaped structures, and the concave blocks (170) are concave-shaped structures. The two are located below the crests and troughs of the wave groove, respectively, and correspond to each other.

8. The cell culture incubator (100) as described in claim 7, characterized in that, Both the protrusion (160) and the concave block (170) are hollow bladder structures, and their bottoms are made of hard material. Both are divided into three spaces, including air chamber one (161), air chamber two (162), air chamber three (163) and chamber one (171), chamber two (172), and chamber three (173).

9. The cell culture incubator (100) as described in claim 8, characterized in that, The air chambers 1 (161), 2 (162), and 3 (163) are connected to an external air pump via independent pipes. They are used to change the volume of the protrusions (160) and concave blocks (170) by inflating or deflating, thereby changing the curvature of the wave crests and troughs of the wave groove, and thus changing the degree of oscillation during the oscillation.

10. The cell culture incubator (100) as described in claim 9, characterized in that, The air chambers 1 (161), 2 (162), 3 (163), 1 (171), 2 (172), and 3 (173) all expand to the same height in the fully expanded state. When the curvature at the trough is the largest, there is no gas in 3 (173) and 2 (172) and they are in a close-fitting state. When the curvature at the crest is the largest, air chambers 1 (161), 2 (162), and 3 (163) are all in a fully expanded state.

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