Artificial breeding anoplophora chinensis biological type dastarcus helophoroides and method applied to prevention and treatment of anoplophora chinensis

Abstract

The invention discloses an artificial breeding anoplophora chinensis biological type dastarcus helophoroides and a method for preventing and treating anoplophora chinensis, which comprises the following steps of: firstly, collecting wild anoplophora chinensis dastarcus adults in the field, feeding and inducing oviposition, storing the oviposition grains, taking out part of the oviposition grains to incubate the anoplophora chinensis biological type dastarcus helophoroides one-year-old larvae, and taking out part of the oviposition grains to incubate the anoplophora chinensis biological type dastarcus helophoroides; the other part of egg grains can be used for manufacturing egg cards for field release for preventing and treating anoplophora chinensis. Then, anoplophora chinensis larvae or barley pest pupae are utilized to breed first-instar larvae of anoplophora chinensis biological type dastarcus helophoroides, and adults are emerged and stored; and finally, part of imagoes are used for repeating the process, and large-scale artificial breeding of the biological dastarcus helophoroides of the anoplophora chinensis is achieved. The other part can be used for field release for preventing and treating anoplophora chinensis. According to the method, biological prevention and control are carried out by utilizing parasitic natural enemy anoplophora chinensis biological type dastarcus helophoroides, the anoplophora chinensis is finally killed through artificial mass propagation and release, and the quantity of pest populations can be controlled at a relatively low level when the anoplophora chinensis is continuously released.

Description

TECHNICAL FIELD

[0001] The present application belongs to the artificial mass breeding and application technology of natural enemy insects, and particularly relates to an artificial breeding of star beetle biological type of Scolytus multistriatus and a method for preventing and treating star beetle. BACKGROUND

[0002] The star beetle (Anoplophora chinensis (Forster)) is distributed in China from Jilin and Liaoning in the north, Gansu and Shaanxi in the west, Fujian and Taiwan in the east, and Guangdong in the south. It is also distributed in Japan, Korea, Myanmar and North Korea. It harms plants such as jute, poplar, willow, elm, locust, walnut, mulberry, red Chinese ash, sorbus, sycamore, acacia, plane tree, and citrus. The female adult carves grooves on the bark to lay eggs, and one female adult can lay about 30 eggs in one generation, and more than 70 eggs in many cases. The adult beetle has a lifespan of 40-50 days, and the flying distance is about 40 m. The newly hatched larvae bore into the tree from the egg-laying site, feed between the tree bark and the wood, form irregular flat tunnels, and the tunnels are filled with feces. After 20-30 days, the larvae start to feed on the wood, often bore upwards to form irregular tunnels, or bore downwards into the roots. There are 1-3 air holes in the tunnels, from which the larvae discharge feces similar to sawdust. The whole larval period lasts for 10 months, the tunnel is 20-60 cm long and 0.5-2.0 cm wide. The larvae harm the tree trunk 20 cm below the ground, accounting for 91.4%, and the tree trunk 20-100 cm above the ground, accounting for 6.3%. The larvae bore into the underground roots, accounting for 23%. The larvae have 6 instars. The mature larvae use wood chips and wood fibers to block the two ends of the tunnel and form a pupal chamber, and pupate in it. The star beetle seriously harms the forest with a stand age of 4-6 years, high canopy density, poor ventilation, poor forest hygiene, and dense weeds in the farmland shelterbelt and patch forest. The star beetle slightly harms the forest with a stand age of less than 2 years or more than 9 years. The main natural enemies of the star beetle are ants, which can invade the tunnels and eat the larvae or pupae. In Zhejiang, there is one species of egg parasitic wasp and one species of earwig that feeds on the star beetle larvae. There is also one species of parasitic peak and Scolytus multistriatus in the larval stage.

[0003] Although the artificial breeding technology of Scolytus multistriatus has been developed in China, Scolytus multistriatus is divided into different biological types. Although the genetic differences between different biological types are small, their living habits and host selection are significantly different. In recent years, we have found star beetle biological type Scolytus multistriatus in the star beetle tunnel in the wild, brought it back to the laboratory for artificial breeding, and used it for the prevention and treatment of star beetle in the wild, which has achieved good prevention and treatment effect. On the contrary, if other biological types of Scolytus multistriatus are mistakenly used to prevent and treat star beetle, it will inevitably lead to poor prevention and treatment effect.

[0004] There is no report on the Anoplophora chinensis bioform of Sclerotarum sp. It is known as the Anoplophora malasiaca bioform of Sclerotarum sp. Although the Anoplophora malasiaca is similar to the Anoplophora chinensis in external morphology, the Anoplophora malasiaca and the Anoplophora chinensis belong to two completely different species. In many places, the Anoplophora malasiaca bioform of Sclerotarum sp. is used to prevent and control the Anoplophora chinensis, which is unreasonable. SUMMARY

[0005] In view of the above problems, the present application provides an artificial breeding Anoplophora chinensis bioform of Sclerotarum sp. and a method for preventing and controlling the Anoplophora chinensis, which can effectively prevent and control the Anoplophora chinensis and is safe to the environment. The Anoplophora chinensis bioform of Sclerotarum sp. collected in the wild is bred in large quantities, and the artificial breeding technology of the Anoplophora chinensis bioform of Sclerotarum sp. is applied to the prevention and control of the Anoplophora chinensis.

[0006] The method for artificial breeding Anoplophora chinensis bioform of Sclerotarum sp. and applying to prevent and control the Anoplophora chinensis provided by the present application has the following steps:

[0007] Step 1: build a rearing box;

[0008] Step 2: collect about 20 wild Anoplophora chinensis Sclerotarum sp. adults in the wild and put them into the rearing box; further put the rearing box into an artificial climate room with a temperature of 25-26℃ and a relative humidity of about 50%; every 3 days, take out the kraft paper on the wood block to observe whether eggs are laid; if eggs are found, proceed to step 3.

[0009] Step 3: take out the kraft paper sheet with eggs and put it into a hatching box; then put it into a incubator, control the temperature at 25-26℃ and the relative humidity at about 50%, and hatch to obtain Anoplophora chinensis bioform of Sclerotarum sp. first instar larvae.

[0010] Step 4: use Anoplophora chinensis larvae / pupae to feed Anoplophora chinensis bioform of Sclerotarum sp. first instar larvae, or use alternative host mealworm pupae to feed Anoplophora chinensis bioform of Sclerotarum sp. first instar larvae, to obtain Anoplophora chinensis bioform of Sclerotarum sp. adults.

[0011] Step 5: put the Anoplophora chinensis bioform of Sclerotarum sp. adults into a storage box, and after 2 weeks of rearing at an indoor temperature, move them to an artificial climate room with a temperature of 5-8℃ and a relative humidity of about 50% for cold storage.

[0012] Step 6: take about 200 Anoplophora chinensis bioform of Sclerotarum sp. adults from step 5 that have been reared for 2 weeks, or cold storage adults, or a combination of both, and perform steps 1-5 to artificially breed and store them in large quantities indoors.

[0013] Based on the artificial breeding of the star beetle biological type of flower and the application of the method for preventing and treating the star beetle, a large number of star beetle biological type of flower egg grains and adults are reserved, so that the egg grains and adults reserved by the method are used for different environments, and two ways of egg card prevention and adult prevention are adopted, specifically as follows:

[0014] For the area with low trees or low-density trees, the star beetle biological type of flower egg card is released to prevent and treat the star beetle, the number of flower egg cards is determined according to the number of fecal orifices on the tree bark, and the number of the two is 1:1.

[0015] Firstly, fold the long rectangular kraft paper in the middle of the long side, and press the folding line.

[0016] Then, the egg-containing kraft paper stored in step 3 is cut into 1cm*1cm size, each with 50-80 eggs;

[0017] Further, the cut kraft paper is adhered to the inner side of the rectangular kraft paper by double-sided adhesive; then the kraft paper is folded along the crease, only the two opposite wide edges of the kraft paper are adhered, and then the wide edges are fixed by stapling to obtain the egg card.

[0018] Finally, the egg card crease side is upward, and the left and right edges of the egg card are fixed by stapling between the bark to make the egg card located in the range of 0-3 centimeters above the fecal orifice.

[0019] (2) For the forest area with high trees or high-density trees, star beetle biological type of flower adult is released to prevent and treat the star beetle.

[0020] Firstly, 10-20 adult insects in step 6 after being fed for two weeks, or adult insects stored in the refrigerator, or a combination of the two are placed in a 2ml centrifugal tube, the centrifugal tube cover is perforated for ventilation, and a three-folded kraft paper sheet is placed in the centrifugal tube.

[0021] Then, find the damaged tree trunk, insert a toothpick obliquely on the bark at a position about 1.7 meters from the tree trunk, open the centrifugal tube cover containing the star beetle biological type of flower adult, and insert the centrifugal tube upside down on the toothpick, so that the star beetle biological type of flower adult crawls from the centrifugal tube to the damaged tree.

[0022] The advantages of the present application are:

[0023] 1. The present application provides an effective natural enemy, i.e., starve beetle biological type of flower flannel mite, for preventing and treating a major forestry and garden pest, i.e., starve beetle. The present application discloses the discovery of the parasitic natural enemy insect, artificial feeding, artificial mass breeding method, biological control technology of releasing eggs and adult insects, and technology of long-term preservation without affecting egg hatching rate and adult egg-laying amount. The present application also discloses the time and releasing technology of releasing egg cards. The present application has remarkable prevention and control effect, and achieves effective and sustainable control of starve beetle without polluting the environment and protecting biological diversity.

[0024] 2. The present application uses the parasitic natural enemy, i.e., starve beetle biological type of flower flannel mite, to carry out biological control by artificially mass breeding and releasing starve beetle flower flannel mite. The natural enemy insect can parasitize and breed in nature, expand the population quantity, and ultimately eliminate starve beetle. Therefore, as long as the releasing is persisted for 3-5 years, the pest population quantity can be controlled at a low level in the later period, and the present application is an environment-friendly technology worthy of promotion. BRIEF DESCRIPTION OF DRAWINGS

[0025] Figure 1 The present application is a method flow chart for artificially breeding starve beetle biological type of flower flannel mite.

[0026] Figure 2 The present application is a structure schematic diagram of a breeding insect box.

[0027] Figure 3 The present application is a structure schematic diagram of a breeding insect wood block. DETAILED DESCRIPTION

[0028] The present application will be further described in detail below with reference to the accompanying drawings.

[0029] The present application is a method for artificially breeding starve beetle biological type of flower flannel mite and applying the method to prevent and treat starve beetle, which comprises a method for artificially breeding starve beetle biological type of flower flannel mite, and a method for preventing and treating starve beetle by using starve beetle biological type of flower flannel mite bred by the method.

[0030] The method for artificially breeding starve beetle biological type of flower flannel mite, as shown in Figure 1 , comprises the following steps:

[0031] Step 1: A breeding insect box is built in a laboratory, as shown in Figure 2 .

[0032] 1) A transparent plastic box with a size of 165mmx105mmx75mm is prepared as the breeding insect box.

[0033] 2) The inner wall of the bottom surface of the breeding insect box is coated with a purple lacquer.

[0034] 3) After the purple lacquer is dry, a hard mesh with a size of 2x2mm is laid on the inner bottom surface of the breeding insect box.

[0035] 4) Put an oviposition block on the gauze screen; the oviposition block is a smooth-surfaced wood block of 85 mm x 40 mm x 30 mm made of Ficus tree. Four layers of kraft paper are placed on the upper surface of the oviposition block, and the centers of the four layers of kraft paper are coincident. The sizes of the four layers of kraft paper from bottom to top are first to fourth layers, the first layer is 75 mm*35 mm, the second layer is 100 mm*50 mm, the third layer is 90 mm*40 mm, and the fourth layer is the same as the first layer, 75 mm*35 mm. A glass slide is placed on the top of the four layers of kraft paper, and the size of the glass slide is 75 mm*25 mm. The glass slide, the kraft paper, and the wood block are bundled together with rubber bands.

[0036] 5) Make a resting room for adult insects.

[0037] A piece of kraft paper with a size of 90 mm*40 mm is folded along the long axis of symmetry, and the two short edges are overlapped. A staple is fixed in the middle of the short edge with a stapler, and the two sides are open. The half-opened edge of the staple is half-opened, forming a U-shaped structure with the top connected together, which is fixed on the inner wall of the bottom surface for the larvae of the flower beetle to crawl in and rest.

[0038] 6) Place a water feeder and a feeding dish in the insect rearing box. The water feeder is a plastic bottle with a length of 44 mm and a diameter of 14 mm, with one end open and the opening plugged with a sponge. Two groups of water feeders are tied together with rubber bands. The feeding dish is a circular bottle cap with a height of 3 mm, which is used to hold the feed of the flower beetle.

[0039] The above-mentioned feed uses star beetle larvae, specifically: one live star beetle larva that has been pre-collected in the wild and stored in a 5-degree refrigerator is placed in a microwave oven and heated for 5 minutes, and then heated again for 5 minutes, making the star beetle larvae crisp and brittle, which can be broken into pieces; every 2 days, the above-mentioned method is used to make and supplement the feed in the feeding dish.

[0040] Step 2: About 20 star beetle bio-type flower beetles collected in the wild are placed in the insect rearing box built in step 1; further, the insect rearing box is placed in an artificial climate room with a temperature of 25-26℃ and a relative humidity of about 50%, and every 3 days, the kraft paper on the wood block is taken out to observe whether there is oviposition. If oviposition is found, the subsequent step 3 of hatching can be carried out, and the kraft paper and glass slide are re-bundled on the wood block according to the method of step 1 sub-step 4) and then placed in the insect rearing box, and step 2 is continued.

[0041] Step 3: Hatching star beetle bio-type flower beetle first instar larvae.

[0042] The cow leather paper piece with the eggs attached is taken out and put into a hatching box, and the box cover is covered; the hatching box is a plastic box with a ventilation hole and a box cover. Then it is put into a culture box, and the temperature is controlled at 25-26 DEG C, and the relative humidity is about 50%, and hatching is carried out, and the hatched star anise biological type Sirex noctilio first instar larvae can be obtained after about 10 days.

[0043] Step 4: breeding star anise biological type Sirex noctilio adults.

[0044] In the present application, two ways are provided for breeding star anise biological type Sirex noctilio first instar larvae, which are specifically as follows:

[0045] Method 1: star anise biological type Sirex noctilio first instar larvae are bred by using star anise larvae (pupae).

[0046] The star anise larvae (pupae) collected from the wild are taken out, a fine hair brush is used, the tip of the fine hair brush is first dipped in distilled water, and the star anise biological type Sirex noctilio first instar larvae 6-9 are connected to the live star anise larvae; if the star anise pupae are connected, 2-3 Sirex noctilio first instar larvae are less connected, and the specific number is determined according to the size of the host. Then the star anise larvae (pupae) connected with the Sirex noctilio first instar larvae are put into a 2ml finger-shaped tube (the tube cover is removed), the finger-shaped tube is tightly plugged with sterilized absorbent cotton, and the purpose of tightly plugging the sterilized absorbent cotton is to prevent the star anise larvae and the Sirex noctilio first instar larvae from crawling out, and to maintain the ventilation inside the finger-shaped tube. The finger-shaped tube is vertically placed in a plastic box, and the plastic box is placed in an artificial climate room with a temperature of 25-26 DEG C and a relative humidity of about 50%. The star anise biological type Sirex noctilio adults can be eclosed after about 30 days.

[0047] Method 2: star anise biological type Sirex noctilio first instar larvae are bred by using substitute host maize worm pupae.

[0048] A 50ml straight cross cover centrifuge tube is used, a 2mm diameter hole is first drilled in the center of the cross cover for ventilation. Then the mature maize worm larvae are put into a centrifuge tube one by one, the cover is buckled, and the plastic basket is put into an artificial climate room with a temperature of 25-26 DEG C and a relative humidity of about 50%, and the maize worms pupate successively after about 2 weeks. The maize worm pupae are not resistant to storage, and the single maize worm pupae should be placed in a refrigerator at about 10 DEG C together with the centrifuge tube, and can be stored for 2 weeks, and can still be used as a substitute host. If the maize worm pupae are picked out from the centrifuge tube and stored in a refrigerator at about 10 DEG C, the body part will become black after 4-5 days of storage, that is, it cannot be used.

[0049] The maize worm pupae (preferably the maize worm pupae that have just pupated for 1-2 days) that have pupated for 1-2 days are taken, and the star anise biological type Sirex noctilio adults can be eclosed by the method of method 1. When the Sirex noctilio first instar larvae are inoculated on the maize worm pupae host, the Sirex noctilio first instar larvae are placed under the legs or head of the maize worm pupae, and the parasitic effect is the best.

[0050] Step 5: Storage of the reared adult insects.

[0051] The newly emerged adult insects can eat their own cocoon as a nutritional supplement, and the flower moth adult insects can be released from the finger tube into the storage box after 40-50 days, and water and food are provided in time.

[0052] The storage box is a transparent plastic box with a lid, with a size of 280mm x 190mm x 180mm, and the inner wall of the bottom surface is coated with shellac paint; after the shellac paint is dry, a layer of hard gauze with the same size as the bottom surface of the box is laid on the bottom surface of the box, with a mesh size of 2x2mm, as an adult insect storage box, which can hold 3000 flower moth adult insects. Five small holes with a diameter of about 1mm are punched on the corners and center of the lid of the adult insect storage box for ventilation. Two insect rearing wood blocks are placed in the storage box, as shown in Figure 3 The insect rearing wood block is a rectangular camphor wood block; a large size groove is opened in the middle of the top surface of the wood block as a resting space for the flower moth during the day. A channel is opened on the front side of the top surface of the wood block, which penetrates through the front side of the wood block and the large size groove, as a channel for the flower moth to enter and exit the resting space; at the same time, a through hole is opened on the front side of the wood block below the channel, which communicates with the large size groove. Four groups of water bottles are also placed inside the storage box to provide water for the flower moth; the water bottles are small plastic bottles with a length of 44mm and a diameter of 14mm, with one end open, the opening is plugged with a sponge, two bottles are tied together as a group with a rubber band to provide water for the flower moth; at the same time, a small piece of filter paper is placed in the storage box for feeding. Since the cost of the borer larvae is high and difficult to collect, the feed used here is artificial feed, which can be:

[0053] Feed 1: yellow mealworm pupa powder

[0054] A large number of yellow mealworm pupae are collected, and those collected every day are frozen in a refrigerator at minus 20 degrees Celsius. When needed, they are taken out and dried in an oven, then ground into powder with a pulverizer, and stored at minus 20 degrees Celsius.

[0055] Feed 2: camphor tree bark.

[0056] The camphor tree bark is collected in the wild, disinfected with ultraviolet light for 2-3 hours, then ground into powder with a pulverizer, and stored at minus 20 degrees Celsius. The raw material 3 is a mixture of various vitamins and mineral components in powder form, stored at minus 20 degrees Celsius. Mix the three raw materials evenly and store them in a sealed box at minus 20 degrees Celsius.

[0057] The newly emerged adult insects are placed in the adult insect storage box and raised in a room for about 2 weeks, then stored in a refrigerator. The cold storage conditions are: temperature 5-8℃, relative humidity 50% in an artificial climate room.

[0058] Step 6: about 200 adult Sirex biotype spalangopanchladii beetles, which are reared for 2 weeks in step 5, or stored in a refrigerator, or both, are used for indoor artificial mass breeding.

[0059] A, the insect rearing box is built according to the method in step 1, and a transparent plastic box with a cover is used in the process, with the size being 280mm*190mm*180mm, and because the number of adult beetles is large, more (6) egg-laying wood blocks and more groups of water drinkers are arranged. At the same time, the feeding dish is replaced by filter paper to place more feed.

[0060] B, about 200 adult Sirex biotype spalangopanchladii beetles are put into the insect rearing box built in sub-step A; further, the insect rearing box is put into an artificial climate room with a temperature of 25-26℃ and a relative humidity of about 50%. The kraft paper on the egg-laying wood block is taken out every 3 days to observe whether eggs are laid. If eggs are found, the subsequent sub-step C of egg storage can be carried out, and the kraft paper and glass sheet are bound on the wood block according to the method in step 1, and then put into the insect rearing box, and sub-step B is continued.

[0061] C, the 4 kraft papers on the wood block are taken out, and the kraft paper with eggs is put into a plastic box, and a water bottle is placed in the plastic box for moisture retention. Then, the kraft paper with eggs is first put into a 15-degree refrigerator for 1-2 days, and then put into an 8-degree refrigerator for storage, and can be stored for about 60 days, which is used for subsequent breeding of adult beetles or release.

[0062] D, the kraft paper with spalangopanchladii eggs is taken out from the 8-degree refrigerator, and about 60 kraft papers are put into a plastic incubation box, 4 holes are drilled around the cover of the incubation box as air holes, and the incubation box is placed in an artificial climate room with a temperature of 25-26℃ and a relative humidity of about 50%, and waits for hatching. Whether the larvae are hatched is observed after 3-5 days, and then observed every day.

[0063] E, the hatched larvae are taken out, and the first instar larvae of Sirex biotype spalangopanchladii are mass bred by using the method 2 in step 5.

[0064] F, the adult beetles are bred according to the method in step 5 and stored, and then can be used for breeding adult beetles or directly released.

[0065] After the Sirex biotype spalangopanchladii beetles are mass bred by the above method in the application, the Sirex beetles are prevented and controlled by the following methods, including egg card prevention and control and adult beetle prevention and control.

[0066] (1) For the areas with lower tree height or lower tree density, the egg card of Sirex biotype spalangopanchladii beetles is used to prevent and control the Sirex beetles.

[0067] At present in our country south Jiangsu, Jiangxi, Zhejiang, Hubei, Beijing, Shanghai, etc. City, star borer mainly harm willow, sycamore trees, etc. According to the number of excrement hole on the surface of willow, sycamore tree trunk to determine the number of release egg card of flower velvet beetle, the number of both is 1∶1.

[0068] The way of making egg card is:

[0069] First, cut the long 10 cm, 5 cm wide rectangular cowhide paper, fold from the middle of the long side (5 cm) to the circumference edge, and press the fold line.

[0070] Then take out the egg-containing cowhide paper stored in step 6 sub step C, cut it into 1 cm*1 cm size, and each has about 50-80 eggs on it;

[0071] Further, the cut cowhide paper is pasted on the inside of the rectangular cowhide paper by double-sided adhesive.

[0072] Finally, fold the cowhide paper along the crease, only make the two opposite wide edges of the cowhide paper fit, and then fix the wide edges by stapling.

[0073] When releasing in the field, fold the egg card crease side up, and fix the left and right edges of the egg card with the tree bark by stapling, so that the egg card is located within 0-3 cm above the excrement hole. In this way, the egg card can be firmly attached to the tree, and rainwater can be prevented from entering in the short term to prevent ants from crawling in and eating the eggs. And through the rough surface of the cowhide paper, newly hatched small larvae can attach to it, crawl out of the paper, and find the pests on the tree.

[0074] In the above egg card release method, when a borer larva bores into the trunk and causes damage, there is only one excrement hole on the surface of the tree, which is obvious and easy to find. However, star borer larvae that harm sycamore trees are different. A star borer larva can see a line of irregular direction of excrement hole on the outer surface of sycamore tree trunk. This is caused by a star borer larva, and it does not need to staple an egg card in each small excrement hole. Instead, it only needs to find the direction of the excrement hole line and find the largest one at one end of the excrement hole, and then staple the egg piece above it.

[0075] (2) For forest areas with lower trees or lower tree density, release star borer bio-type flower velvet beetle adults to control star borer.

[0076] When star borer excrement holes are found on taller trees, the height of the tree makes it impossible for humans to staple egg cards near the excrement holes, so star borer bio-type flower velvet beetle adults are directly released for control.

[0077] First, put 10-20 adult insects, which have been reared for two weeks in step 6 sub-step F, or stored in a refrigerator, or both, into a 2ml centrifuge tube, which is covered with a hole for ventilation, and put a piece of three-folded cow leather paper into the tube.

[0078] Subsequently, find the victim tree trunk, and at a position about 1.7 meters from the trunk, insert a toothpick into the bark obliquely, open the centrifuge tube cover containing the adult insects of the biological type of the star beetle flower flannel beetle, and insert the tube upside down on the toothpick so that the toothpick is inside the tube, and then the star beetle flower flannel beetle adult insects can crawl from the centrifuge tube to the victim wood.

[0079] As for the way of releasing adult insects, the release time is preferably after the afternoon, and the release should not be done on a rainy day. After releasing the natural enemies, the area should not be sprayed with pesticides for a short period of time. And after the release is completed, make good test marks to avoid artificial damage to the egg cards or the adult insect release tube, and affect the control effect.

Claims

1. A method for artificially breeding the longhorn beetle biotype *Aegilops spp.*, characterized in that: The specific steps are as follows: Step 1: Set up the insect rearing box; Step 2: Collect 20 wild-caught adult longhorn beetle flower velvet beetles and place them in a rearing box; further place the rearing box in an artificial climate chamber with a temperature of 25-26℃ and a relative humidity of 50%, and remove the kraft paper on the wooden block every 3 days to observe whether eggs have been laid; if eggs are found, proceed to Step 3. Step 3: Remove the kraft paper with the eggs attached and place it in the incubation box; then place it in an incubator, control the temperature at 25-26℃ and the relative humidity at 50%, and hatch to obtain the first instar larvae of the longhorn beetle biotype flower velvet beetle. Step 4: Use longhorn beetle larvae / pupae to feed first-instar longhorn beetle biotype flower velvet beetle larvae, or use supernatant mealworm pupae to feed first-instar longhorn beetle biotype flower velvet beetle larvae to obtain longhorn beetle biotype flower velvet beetle adults. Step 5: Place the adult longhorn beetle biotype flower velvet beetle into a storage box, provide water and feed, and raise it at an indoor temperature for 2 weeks. Then, move it to an artificial climate chamber with a temperature of 5-8℃ and a relative humidity of 50% for cold storage. Step 6: Take 200 adult beetles of the longhorn beetle biotype, which have been raised for 2 weeks in Step 5, or have been refrigerated, or a combination of both, and return to Steps 1-5 for large-scale indoor artificial breeding and storage. In step 6 above, during the execution of steps 1-5, the following characteristics are also present: A. During step 3, place the eggs in a box and put a water bottle in the box to keep them moist; then store them in a 15-degree refrigerator for 1-2 days, and then store them in an 8-degree refrigerator; subsequently, take out 60 pieces of kraft paper with the flower-like parasitic eggs from the 8-degree refrigerator for the next steps. B. In step 4, only the pupae of the alternative host, the giant mealworm, are used to feed the first instar larvae of the Asian longhorn beetle, the flower-like parasitic beetle.

2. A method for controlling the Asian longhorn beetle by artificially breeding the biotype of the flower-like parasitic beetle according to claim 1, characterized in that: This includes two methods: egg control and adult control. (1) For areas with low-growing or low-density trees, release the longhorn beetle biotype flower velvet egg card to control the longhorn beetle. The number of flower velvet egg cards to be released is determined according to the number of frass holes on the trunk surface, and the ratio of the two is 1:

1. First, fold a rectangular piece of kraft paper in half along the middle of its long side and press to create a fold line; Then take out the kraft paper containing eggs stored in step 3, cut it into 1cm*1cm pieces, each with 50-80 eggs; Next, the cut kraft paper is glued to the inside of the rectangular kraft paper using double-sided tape; then the kraft paper is folded along the creases, so that only the two opposite wide edges of the kraft paper are touching, and then the wide edges are fastened together with staples to obtain the egg card. Finally, with the folded side of the egg card facing upwards, fix the egg card to the bark by staples along the left and right edges, so that the egg card is positioned within 0-3 cm above the manure outlet. (2) For forest areas with tall trees or high tree density, release adult Asian longhorn beetle biotypes to control Asian longhorn beetles; First, place 10-20 adult insects (either those raised for two weeks in step 6, those stored in cold storage, or a combination of both) into a 2ml centrifuge tube. Make a hole in the cap of the centrifuge tube for ventilation and place a three-fold piece of kraft paper inside the centrifuge tube. Subsequently, the affected tree trunk was located, and a toothpick was inserted obliquely into the bark at a position 1.7 meters from the trunk. The cap of the centrifuge tube containing the adult flower velvet beetle of the Asian longhorn beetle was opened and inserted upside down into the toothpick, causing the adult flower velvet beetle to crawl from the centrifuge tube onto the affected tree.

3. The method for artificially breeding the longhorn beetle biotype *Aegilops spp.* as described in claim 1, characterized in that: The insect rearing box is a transparent box with dimensions of 165mm×105mm×75mm. The inner wall of the bottom of the box is coated with shellac paint and covered with a rigid mesh with a mesh size of 2×2mm. The mesh is used to place insect rearing wood blocks and adult insect resting areas. A water dispenser and a feed dish are also provided inside the box.

4. The method for artificially breeding the longhorn beetle biotype *Aegilops spp.* as described in claim 3, characterized in that: The insect-rearing wooden block is a smooth-surfaced egg-laying block with dimensions of 85mm×40mm×30mm. Four layers of kraft paper are stacked on the upper surface of the egg-laying wooden block from bottom to top, with dimensions of 75mm*35mm, 100mm*50mm, 90mm*40mm, and 75mm*35mm respectively. A glass slide with dimensions of 75mm*25mm is placed on top of the four layers of kraft paper, and the glass slide, kraft paper, and wooden block are bound and fixed with rubber bands.

5. The method for artificially breeding the longhorn beetle biotype *Aegilops spp.* as described in claim 3, characterized in that: The adult insect resting room is a 90mm*40mm piece of kraft paper. After being folded in half along the long side axis of symmetry, the corresponding short sides are fixed in the middle to form a U-shaped structure with the tops connected together.

6. The method for artificially breeding the longhorn beetle biotype *Aegilops spp.* as described in claim 1, characterized in that: In step 2, star longhorn beetle larvae are used as feed. A live star longhorn beetle larva, which was collected in the wild and stored in a 5-degree refrigerator, is placed in a microwave oven and heated for 5 minutes, and then heated for another 5 minutes to make the star longhorn beetle larvae crispy and broken into pieces. The above method is used to prepare the feed every 2 days and supplemented in the rearing dish.

7. The method for artificially breeding the longhorn beetle biotype *Aegilops spp.* as described in claim 1, characterized in that: In step 5, the feed in the storage box is artificial feed; the artificial feed is mealworm powder that has been dried and ground into powder; or sycamore tree powder that has been disinfected by ultraviolet light for 2-3 hours and then ground into powder; or nutritional powder that is a mixture of vitamins and minerals ground into powder.

8. The method for artificially breeding the longhorn beetle biotype *Aegilops spp.* as described in claim 1, characterized in that: In step 5, the storage box is made of transparent material, measuring 280mm×190mm×180mm. The inner wall of the bottom surface of the box is coated with shellac paint. After the shellac paint dries, a layer of hard mesh with the same size as the bottom surface of the box is laid on the bottom surface of the box, with a mesh size of 2×2mm. A small hole is made at each of the four corners and the center of the storage box lid for ventilation. Two insect-rearing wooden blocks are placed inside the storage box. The insect-rearing wooden blocks are rectangular paulownia wood blocks. A large groove is opened in the center of the top surface of the wooden block, which serves as a resting space for the flower beetle during the day. A channel is opened on the front side of the top surface of the wooden block, connecting the front side of the wooden block to the large groove, which serves as a passage for the flower beetle to enter and exit the resting space. At the same time, a through hole is opened on the front side of the wooden block below the channel, which connects to the large groove.

9. The method for artificially breeding the *Acer truncatum* biotype as described in claim 1, characterized in that: During the rearing of first-instar larvae of the longhorn beetle biotype *Pteris vittata* using substitute host *Mealworm* pupae: Using 50ml straight-mouth cross-shaped centrifuge tubes, first drill a hole in the center of the cross-shaped cap for ventilation; then place a mature mealworm larva into a centrifuge tube individually, seal the cap, place the tube in a plastic basket, and put the basket in an artificial climate chamber at a temperature of 25-26℃ and a relative humidity of 50%. The mealworms will pupate successively over two weeks; carefully preserve the single mealworm pupa along with the centrifuge tube in a 10-degree refrigerator; when inoculating the mealworm pupa with a first-instar larva of the longhorn beetle biotype *Pterocarya stenoptera*, the inoculation site should be below the legs or on the head of the mealworm pupa.

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