Small-molecule organic liquid feed raw material and production method thereof

Abstract

The invention provides a micromolecular organic liquid feed raw material and a production method thereof, and belongs to the technical field of organic waste recycling. According to the method, the prominent problems of pathogenic bacteria, viruses, worm egg pathogen microorganisms and the like of liquid feed raw materials, which cannot be solved by a fermentation method, can be completely eliminated, and the liquid feed can be stored for a long time without deterioration. Through continuous processes such as an activation reaction, a fermentation reaction, a biochemical reaction and a shearing reaction, feed raw material nutrient substances are subjected to micromolecularization, protein is converted into micromolecular amino acid peptides, crude fibers are converted into saccharides, fat is converted into fatty acid, glycerin and other nutrient substances which are more convenient for animals to quickly absorb, and the use amount of complete feed can be directly reduced. The feed cost is greatly reduced.

Description

Technical Field

[0001] This invention relates to the field of waste resource utilization technology, and in particular to a small molecule organic liquid feed ingredient and its production method. Background Technology

[0002] With global population growth and the development of intensive agriculture, how to achieve efficient and high-value utilization of agricultural by-products and organic waste has become an important issue in the field of resources and environment. The core of the concept of "resource utilization" lies in transforming materials that might otherwise be discarded or used inefficiently into new products or energy with economic value through physical, chemical, or biological methods, thereby forming a circular economy model that reduces environmental burden while creating new economic benefits.

[0003] In aquaculture and livestock farming, feed costs account for a significant proportion of total production costs. Developing new feed resources and reducing feed costs are key directions for the industry's sustainable development. Liquid feed products, as an important form of feed, are widely used in aquaculture due to their advantages such as good palatability, ease of mixing and adding nutrients, and suitability for pipeline transportation and automated feeding. These products typically refer to fluid feeds with low solid content, formed by mixing, fermenting, enzymatic hydrolysis, or other processing of various feed ingredients (such as molasses, oils, residues, and trace elements). They not only provide moisture and basic nutrients but also often serve as an excellent energy source and carrier of functional components.

[0004] Existing technologies for producing liquid feed often involve steps such as crushing, conditioning, fermentation, and sterilization. However, these traditional methods still present numerous challenges in terms of processing efficiency, nutrient retention and degradation, energy consumption control, and the continuity and stability of the production process. For example, how to achieve efficient and uniform release and conversion of nutrients from complex raw materials, how to precisely control key parameters in the process to ensure consistent product quality, and how to design low-energy, intensive process flows remain areas for continuous exploration and improvement by those skilled in the art. Summary of the Invention

[0005] The purpose of this invention is to provide a small molecule organic liquid feed raw material and its production method, which not only realizes the resource utilization of waste, but also enables the produced product to have excellent performance.

[0006] To achieve the above-mentioned objectives, the present invention provides the following technical solution: This invention provides a method for producing small molecule liquid biological feed ingredients, comprising the following steps: S1. The biomass raw material is subjected to impurity removal and activation pulping treatment to obtain activated pulp; S2. The activated slurry is subjected to heating and fermentation treatment to obtain fermented slurry; S3. The fermented slurry is subjected to a biochemical reaction treatment to obtain a biochemical slurry; S4. The biochemical slurry is subjected to a closed-loop pressure cyclic shear reaction treatment, and filtered to obtain a small molecule filtrate and residual solids. The small molecule filtrate is the small molecule liquid biological feed raw material.

[0007] Preferably, the biomass raw materials include one or more of the following: crop straw, forage, grain chaff, brewing lees, soy sauce residue, vinegar residue, sugar residue, fruit residue, soybean residue, rapeseed, peanut, cottonseed, oil residue, kitchen waste, livestock and poultry manure, aquatic product processing by-products, fish and shrimp waste, fly larvae and insects, algae, food processing waste, fruit and vegetable processing residues, forestry processing by-products, and traditional Chinese medicine residues.

[0008] Preferably, in S1, the activated pulping treatment is a mechanical pulping treatment; The rotation speed of the mechanical pulping process is 100~1200 rpm; The mechanical pulping process takes 30-60 minutes. In S1, during the mechanical pulping process, the dry matter concentration of the liquid is controlled to be 10%~30%.

[0009] Preferably, in S2, the heating and fermentation process includes: First stage of preheating: The activated slurry is heated to 65~75℃ and kept at that temperature for 20~40 minutes; The second stage is cooling and fermentation: the pretreated material is cooled to 30~35℃ and a fermentation agent is added to carry out the fermentation reaction.

[0010] Preferably, the fermentation agent includes Bacillus licheniformis preparation and Saccharomyces cerevisiae powder; The fermentation reaction is carried out at a temperature of 30~35℃; The fermentation reaction takes 30 to 120 minutes.

[0011] Preferably, in S3, the biochemical reaction treatment is a catalytic hydrolysis reaction carried out under acidic conditions; The pH value of the catalytic hydrolysis reaction is 2.5~4.0; The temperature of the catalytic hydrolysis reaction is 80~140℃; The catalytic hydrolysis reaction takes 2 to 6 hours.

[0012] Preferably, in S4, the closed-loop shear reaction filtration process is implemented in a closed loop consisting of a high-speed shear disperser and a filter; The operating pressure of the closed-loop circuit is 0.4~1MPa; The high-shear disperser operates at a speed of 3000~12000 rpm; The filter has a filtration precision of 100~300 mesh; In S4, the solids obtained from filtration are returned to the biochemical reaction treatment step in S3 to participate in the reaction again or undergo environmental protection treatment. The production method further includes step S5: concentrating the small molecule filtrate obtained in S4 to obtain a concentrated liquid with a solid content of 25% to 50%.

[0013] Preferably, sensors are set up in one or more of steps S1, S2, S3, and S4 for real-time monitoring, and the processing parameters of the corresponding steps are adjusted according to the monitoring results; The monitoring includes monitoring one or more of the following: slurry moisture content, particle size, amount of prepared materials added, pH value, temperature, pressure, shear force, rotation speed, filtration rate, energy consumption, weight, and time. Preferably, a pressure buffer tank is provided in the biochemical reaction treatment of S3 and / or the closed-loop reaction treatment of S4 to regulate the pressure fluctuations generated during the reaction process. And / or, steps S1, S2, S3, and S4 are connected by one or more of the following methods: pipes, pumps, valves, and screw conveyors, to achieve continuous production; And / or, a built-in and / or external heating pump is installed in the slurry container, wherein the heater is one or a combination of several of the following: a resistance heater, an electromagnetic heater, or an external heat pump heating device, for energy-saving heating of the material.

[0014] The present invention also provides a small molecule liquid biological feed ingredient produced by the above-described production method.

[0015] The beneficial effects of this invention are: This invention provides a small-molecule liquid biological feed and its production method, belonging to the field of waste resource utilization technology. This invention not only completely eliminates the prominent problems of bacteria, viruses, and insect eggs in liquid feed raw materials that cannot be solved by fermentation methods, but also allows the liquid feed to be stored for a longer period without spoilage. Through continuous processes such as activation, fermentation, biochemical reactions, and shearing reactions, the nutrients in the feed raw materials are broken down into small molecules, proteins are transformed into small-molecule amino acids and peptides, crude fiber into carbohydrates, and fats into fatty acids and glycerols. This facilitates the rapid absorption of nutrients by animals and directly reduces the amount of complete feed required, significantly lowering feed costs.

[0016] This invention also employs energy-saving methods and reaction technologies such as segmented and stepped heating and pressurization, gradient stirring / pump circulation stirring, staged heat recovery circulation, and saturated water phase change, so that the temperature of the initial raw materials does not drop during the biochemical reaction process, ensuring the stability, balance, continuity and fullness of the biochemical reaction.

[0017] The method for producing small-molecule liquid biological feed of the present invention is also applicable to the production of aquatic feed or feed additives, and agricultural, livestock and poultry feed or feed additives from biomass such as soybeans, peanuts, fruits, vegetables, straw, as well as marine miscellaneous fish, discarded fish and shrimp, black slugs, fly larvae and insects. Attached Figure Description

[0018] Figure 1 A schematic diagram of the basic process for producing small molecule liquid biological feed; Figure 2 This is a schematic diagram of the overall architecture of the complete set of devices adapted to the method of the present invention. Detailed Implementation

[0019] This invention provides a method for producing small molecule liquid biological feed, comprising the following steps: S1. The biomass raw material is subjected to impurity removal and activation pulping treatment to obtain activated pulp; S2. The activated slurry is subjected to heating and fermentation treatment to obtain fermented slurry; S3. The fermented slurry is subjected to a biochemical reaction treatment to obtain a biochemical slurry; S4. The biochemical slurry is subjected to closed-loop shear reaction filtration treatment to separate small molecule filtrate and residual solids. The small molecule filtrate is the small molecule liquid biological feed.

[0020] Preferably, the biomass raw materials include one or more of the following: livestock and poultry manure, kitchen waste, crop straw, grain husks, brewing lees, soy sauce residue, vinegar residue, sugar residue, soybean residue, aquatic product processing by-products, algae, food processing waste, fruit and vegetable processing residues, traditional Chinese medicine residues, and forestry processing by-products.

[0021] In this invention, the poultry manure includes one or more of the following: chicken manure, duck manure, goose manure, pigeon manure, quail manure, etc. In this invention, the kitchen waste includes one or more of the following: food waste, kitchen scraps, food residues, and waste cooking oil. In this invention, the crop straw is one or more of the following: rice straw, wheat straw, corn straw, sorghum straw, cotton straw, etc. In this invention, the grain husks include one or more of the following: rice husks, wheat bran, rice bran, peanut shells, sunflower seed shells, and bean pods. In this invention, the brewing residue includes one or more of the following: beer residue, baijiu residue, wine residue, and huangjiu residue. In this invention, the soy sauce residue includes one or more of the following: soybean soy sauce residue, defatted soybean meal soy sauce residue, etc. In this invention, the vinegar residue is one or more of, such as rice vinegar residue, wheat bran vinegar residue, etc. In this invention, the sugar residue includes one or more of sugarcane bagasse, beet pulp, molasses, etc. In this invention, the soybean residue includes one or more of the following: tofu residue, soybean milk residue, soybean meal, etc. In this invention, the aquatic product processing by-products include one or more of the following: fish scales, fish viscera, fish heads, fish bones, shrimp heads, shrimp shells, crab shells, shellfish viscera and shells; In this invention, the algae include one or more of Spirulina, Chlorella, Scenedesmus, and large seaweed; In this invention, the food processing waste includes one or more of the following: monosodium glutamate bacterial protein, citric acid residue, yeast residue, breadcrumbs, and expired pastries. In this invention, the fruit and vegetable processing residue includes one or more of the following: fruit peel, fruit pit, fruit pomace, vegetable root, stem and leaf residue, and juice processing residue. In this invention, the medicinal residue is, for example, the residue extracted from plant-based medicinal materials or medicinal and edible crops; In this invention, the forestry processing by-products include one or more of sawdust, wood chips, bark, bamboo powder, bamboo shavings, etc.

[0022] Preferably, in S1, the activated pulping treatment is a mechanical pulping treatment; The rotation speed of the mechanical pulping process is 100~500 rpm; The mechanical pulping process takes 10-60 minutes. In S1, during the mechanical pulping process, the dry matter concentration of the liquid is controlled to be 5% to 15%.

[0023] Preferably, in S2, the heating and fermentation process includes: The first stage is pre-treatment by heating: the activated slurry is heated to 65-75°C and kept at this temperature for 20-40 minutes; the second stage is cooling and fermentation: the pre-treated material is cooled to 30-35°C, and a fermentation agent is added to initiate the fermentation reaction. Preferably, the fermentation agent includes Bacillus licheniformis preparation and Saccharomyces cerevisiae powder; the fermentation reaction temperature is 30-35°C; and the fermentation reaction time is 3-6 hours.

[0024] Preferably, in S3, the biochemical reaction treatment is a catalytic hydrolysis reaction carried out under acidic conditions; the pH value of the catalytic hydrolysis reaction is 2.5~4.0; the temperature of the catalytic hydrolysis reaction is 80~90℃; and the time of the catalytic hydrolysis reaction is 2~6 hours.

[0025] Preferably, in S4, the closed-loop shear reaction filtration process is implemented in a closed loop consisting of a high-shear disperser and a dynamic membrane filter; the working pressure of the closed loop is 0.4~0.8 MPa; the rotation speed of the high-shear disperser is 2000~3000 rpm; and the filtration accuracy of the dynamic membrane filter is 100~300 mesh. In step S4, when the concentration of residual solids in the closed loop reaches 20% to 30%, some of the residual solids are discharged; 40% to 60% of the discharged residual solids are returned to the biochemical reaction treatment step in step S3 to participate in the reaction again; the production method also includes step S5: concentrating the small molecule filtrate obtained in step S4 to obtain a concentrated liquid with a solid content of 30% to 50%.

[0026] Preferably, sensors are installed in one or more steps S1, S2, S3, and S4 for real-time monitoring, and the processing parameters of the corresponding steps are adjusted according to the monitoring results; the monitoring includes monitoring one or more of the following: slurry concentration, particle size, temperature, pH value, and residual solid content; preferably, a pressure buffer tank is installed in the biochemical reaction treatment of S3 and / or the closed-loop reaction treatment of S4 to regulate the pressure fluctuations generated during the reaction; and / or, the steps S1, S2, S3, and S4 are connected by one or more of the following methods: pipelines, pumps, valves, and screw conveyors to achieve continuous production; and / or, a heater is built into the slurry container, which is one of the following: a resistance heater, an electromagnetic heater, or a heat pump heating device, for directly heating the material.

[0027] This invention also provides a small-molecule liquid biological feed produced by the above-described production method. In this invention, the small-molecule liquid biological feed can be mixed with conventional feed for feeding, further improving the quality of animal products.

[0028] The technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0029] Example 1 Combination Figure 1 The diagram shows a basic flow chart of a method for producing small molecule liquid biological feed. The method provided in this embodiment includes the following steps: 1. The received biomass raw materials are activated and pulped to obtain activated pulp; When the biomass raw material is poultry manure such as chicken manure, the coarser stone powder and shell powder in the chicken manure are separated and removed by the impurity removal crushing unit and the impurity removal separator. The crushing unit uses a fine crusher to obtain relatively pure poultry manure, which is then sent to a double spiral mixing pulper. The dry matter concentration of the liquid is controlled at about 10%, and mechanical pulping is carried out at 300 rpm for 20 minutes at room temperature. The staggered spiral blades in the pulper shear, knead and loosen the material, destroy the fiber bundle structure, and fully activate the material to obtain activated pulp. When the biomass raw material is kitchen waste, a separation machine is mainly used for impurity removal and crushing. This mainly removes plastics, disposable chopsticks, and other uncertain impurities from the kitchen waste. The crushing unit uses a coarse crusher and a fine crusher to obtain a relatively pure kitchen waste slurry. This slurry is then fed into a hydraulic pulper with a fly knife and a bottom knife. The dry matter concentration of the slurry is controlled at about 10%. The slurry is mechanically pulped at 200 rpm for 30 minutes at room temperature. During the pulping process, an appropriate amount of room temperature water can be added according to the fluidity of the slurry. The intense hydraulic shearing and friction generated by the fly knife and the bottom knife fully crushes, mixes, and activates the organic components, resulting in an activated slurry.

[0030] 2. The activated slurry is transferred to the batching and heating fermentation tank and circulated and stirred to ensure uniform heating, balanced fermentation reaction, and homogeneous mixing, which is more conducive to subsequent biochemical reactions. This step employs a two-stage temperature-controlled fermentation process: The first stage is preheating: After the activated slurry is pumped into the batching and heating fermentation tank, 75°C hot water is first introduced to heat the jacket / coil, uniformly raising the temperature of the material in the tank to 70°C at a rate of 2°C per minute, and maintaining this temperature for 30 minutes. This process can effectively inactivate miscellaneous bacteria and reduce competition from harmful microorganisms.

[0031] The second stage is cooling fermentation: After pretreatment, the system switches to cooling water circulation, reducing the material temperature to 32°C at a rate of 1.5°C per minute. Once the temperature stabilizes at 32°C, a compound fermentation agent is added through a sterile feeding port. This agent is a mixture of 0.1% Bacillus licheniformis preparation and 0.1% Saccharomyces cerevisiae powder.

[0032] Fermentation process parameter control: Chicken manure slurry: Maintain a temperature of 32±0.5℃ and a relative humidity of 75%, stir at a speed of 40 revolutions per minute, and ferment for 5 hours.

[0033] Kitchen waste slurry: Maintain a temperature of 32±0.5℃ and a relative humidity of 65%, stir at a speed of 30 revolutions per minute, and ferment for 4 hours.

[0034] 3. The fermented slurry is subjected to a biochemical reaction to obtain a biochemical slurry; The fermented slurry was pumped into a tubular preheater, where it was uniformly preheated to 85°C using indirect steam heating. The preheated slurry then entered a biochemical reactor equipped with an online pH monitoring and automatic control system. A 20% dilute sulfuric acid solution was slowly added to the reactor using a metering pump to precisely adjust the pH of the slurry to 3.0. The reaction temperature was maintained at 85°C, and the catalytic hydrolysis reaction was carried out under these acidic, high-temperature conditions. The stirring speed was maintained at 50 revolutions per minute throughout the reaction, with a total reaction time of 4 hours. After the reaction, a completely liquefied biochemical slurry was obtained.

[0035] 4. The biochemical slurry is subjected to closed-loop shear reaction filtration to separate the generated small molecule filtrate and residual solids.

[0036] The slurry, after completing the biochemical reaction, is pumped to a closed-loop shear filtration system at a flow rate of 5 cubic meters per hour using a screw pump. This system consists of a high-shear disperser (2900 rpm, 45 kW) and a dynamic membrane filter (200 mesh). The slurry circulates within the loop at a working pressure of 0.6 MPa. The high-shear disperser mechanically shears and breaks down residual solids in the slurry, promoting the further release of smaller molecules.

[0037] During the circulation process, the dynamic membrane filter continuously separates small molecule filtrate (solid content ≤3%), which is collected in a temporary storage tank for subsequent concentration. Residual solids that fail to pass through the filter (mainly lignin, incompletely decomposed cellulose, etc.) remain in the loop and participate in the cyclic shearing.

[0038] When the concentration of residual solids in the loop reaches 25%, a portion of the residue is discharged through the automatic control system: 60% is returned to the biochemical reactor to participate in the reaction again, and 40% is transported to an environmental treatment system (such as incineration or composting). The entire closed-loop shear reaction filtration process continues for 3 hours until more than 90% of the convertible substances are separated and extracted.

[0039] During a certain operation, under continuous pumping conditions, the activated slurry was pumped to the fermentation and heating treatment tank at a flow rate of 4 cubic meters per hour, and kept at 55°C for 5 hours with stirring to ensure that the activated slurry was uniformly mixed and heated for fermentation reaction.

[0040] The fermented slurry, after being uniformly mixed and heated, is continuously pumped to a biochemical reaction tank. After the reaction, it is transported to a closed-loop shear filtration circuit for cyclic shear reaction. Mechanical shearing is performed through a high-shear disperser, while solid-liquid separation occurs in a dynamic membrane filter, separating small molecule filtrate and residual solids. The small molecule filtrate is sent to a vacuum concentration system to be concentrated to a solid content of 40% before being packaged. The residual solids are recycled or treated in an environmentally friendly manner according to the above proportions.

[0041] This step employs a novel circulating shear filtration loop. The shear force greatly promotes the contact reaction of various nutrients in the slurry, improving biochemical reaction efficiency and small molecule yield. Furthermore, the fully liquefied biochemical slurry does not leak energy during cyclic shearing under pressure, resulting in higher and more complete reaction efficiency. Generally, in the process of treating one batch of food waste, for example, 3 tons of food waste, the total processing time is 6 hours. The system can operate continuously within these 6 hours until completion without downtime. This is not only due to the thorough degradation of the slurry in the circulating reaction mechanism but also to the intensive mixing, heating, reaction, and filtration processes. The compact equipment reduces material transfer, lowers energy consumption, and reduces space requirements.

[0042] Example 2 Based on the process of Example 1, this example provides the following improvements: sensors are added to the activation pulping, fermentation reaction, biochemical reaction and closed-loop reaction stages for real-time monitoring; The real-time monitoring results are uploaded to the host computer, and adjustments are made to the activation pulping, fermentation reaction, biochemical reaction, and closed-loop reaction stages according to the feedback instructions from the host computer.

[0043] Figure 2 The schematic diagram of the overall architecture of the complete set of devices adapted to the method of the present invention is shown. Sensors are added to the raw material storage tank, the sorting and impurity removal pulverizing subsystem, the mixing unit biochemical accelerator tank, the raw material pre-processing subsystem, the heating and exchange subsystem, the biochemical reaction subsystem, the circulating shear closed circulation system, and the auxiliary material subsystem, respectively. These sensors are electrically or communicatively connected to the intelligent control system of the host computer to realize real-time monitoring of the above subsystems or tanks, so as to control the preset reaction logic to adjust the above reactions.

[0044] For example, the particle size of the received biomass raw materials can affect the uniformity of the primary bio-slurry and the efficiency of the biochemical reaction. Therefore, the particle size can be adjusted by activation and pulverization based on the real-time particle size transmitted by the sensor. Also, if the proportion of incompletely degraded residual solids exceeds 20%, the shearing force or shearing intensity in the closed-loop shearing system can be adjusted to reduce the proportion of incompletely degraded residual solids to below a preset 1%. The real-time monitoring employed in this embodiment significantly improves the intelligent control of the overall equipment.

[0045] During a particular operation, the concentration of the slurry is monitored during the activation and pulping process to ensure it remains within a preset range. In this invention, excessively high slurry concentration can cause adhesion, hindering the overall process, while excessively low concentration results in higher energy consumption during the subsequent concentration of the small molecule filtrate. Therefore, by monitoring the slurry concentration, overall energy consumption is stabilized and continuous production is maintained while maximizing the output of the small molecule filtrate.

[0046] Example 3 Based on the process of Example 1, this example provides the following improvements: The closed-loop shear filtration system includes a pressurization subsystem, two-stage or multi-stage cyclic shearing, concentration, metering and dispensing, and a biochemical reaction subsystem electrically connected to heating equipment, stirring equipment, circulating pump equipment, and pressurization equipment. The cyclic shearing includes secondary batching (including auxiliary materials and incompletely degraded residual solids, etc.), cyclic shearing grinding, centrifugal filtration, and stabilizing dispersion equipment.

[0047] Example 4 Based on the process of Example 1, this example provides the following improvements: In this embodiment, a pressure buffer tank is provided in the biochemical reaction stage and the closed-loop reaction stage to regulate the pressure fluctuations generated in the biochemical hydrolysis reaction stage and the closed-loop reaction stage.

[0048] Example 5 Based on the process of Example 1, this example provides the following improvements: The activation and pulping stage, the heating and fermentation stage, the biochemical reaction stage, and the closed-loop reaction stage are connected by pipelines to achieve continuous production.

[0049] Example 6 Based on the process of Example 1, this example provides the following improvements: Pumps connect the activation pulping stage, heating fermentation stage, biochemical reaction stage, and closed-loop reaction stage to achieve continuous production.

[0050] Example 7 Based on the process of Example 1, this example provides the following improvements: The activation and pulping stage, the heating and fermentation stage, the biochemical reaction stage, and the closed-loop reaction stage are connected by valves to achieve continuous production.

[0051] Example 8 Based on the process of Example 1, this example provides the following improvements: The activation pulping stage, heating fermentation stage, biochemical reaction stage, and closed-loop reaction stage are connected by screw conveyors to achieve continuous production.

[0052] Example 9 Based on the process of Example 1, this example provides the following improvements: The activation and pulping stage, the heating and fermentation stage, the biochemical reaction stage, and the closed-loop reaction stage are connected by a circulating pump to achieve continuous production.

[0053] Example 10 Based on the process of Example 1, this example provides the following improvements: A resistance heater is built into the slurry container to achieve direct, rapid, and precise heating of the material.

[0054] Example 11 Based on the process of Example 1, this example provides the following improvements: An electromagnetic heater is built into the slurry container to achieve direct, rapid, and precise heating of the material.

[0055] Example 12 Based on the process of Example 1, this example provides the following improvements: A heat pump heating device is built into the slurry container to achieve direct, rapid, and precise heating of the material.

[0056] Example 13 Based on the process of Example 1, this example provides the following improvements: An integrated solenoid valve is installed at the outlet of the slurry pump to adjust the slurry outlet rate via a host computer.

[0057] Example 14 Based on the process of Example 1, this example provides the following improvements: A solenoid valve is integrated into the slurry circulation pipeline to adjust the slurry outlet rate via a host computer.

[0058] Example 15 Based on the process of Example 1, this example provides the following improvements: After impurity removal, the coarsely selected biomass raw materials are transported to the activated impact pulper via a screw conveyor.

[0059] Experimental Example Based on the scheme in Example 1, the following performance testing experiments of small molecule liquid feed ingredients were conducted: This experiment aims to evaluate the safety, production efficiency, environmental friendliness, and palatability of the small-molecule liquid feed ingredients produced by this invention through direct testing and animal feeding applications. Specifically, it includes verifying the removal efficiency of pathogens and toxins from biomass raw materials (chicken manure, waste fish and shrimp) after activation, pulping, fermentation, and biochemical reactions; recording the production process duration and ingredient flexibility; monitoring gas emissions during production; and evaluating product palatability through animal preference experiments.

[0060] II. Experimental Materials and Methods (a) Experimental materials Small molecule liquid feed ingredients: Chicken manure-based liquid feed (preparation method as in Example 1, crude protein 24.68%, small molecule protein peptides 76%, crude fat 4.57%, small molecule fatty acids 23.26%, glycerol 1.31%, crude fiber 2.13%).

[0061] Waste fish and shrimp-based liquid feed (preparation method as in Example 1, crude protein 28.76%, small molecule protein peptides 76%, crude fat 6.28%, small molecule fatty acids 5.12%, glycerol 1.10%, crude fiber 3.46%).

[0062] Sixty healthy weaned piglets (Duroc-Landrace-Large White crossbred) with an initial weight of (8.50±0.75) kg were randomly divided into three groups of 20 piglets each.

[0063] Testing reagents and instruments: Microbiological testing: culture medium for total bacterial count, Escherichia coli, and Salmonella; ELISA kit for mycotoxins (aflatoxin B1, vomitoxin).

[0064] Gas detection: Portable multi-gas analyzer (can detect NH3, H2S, CH4, CO2).

[0065] Palatability test: dual-trough feeding device.

[0066] (II) Experimental Design 1. Safety testing (direct testing) (1) Detection of microbial and toxin residues Sample pretreatment: Take 500 g each of chicken manure-based and waste fish and shrimp-based liquid feed and aseptically dispense them into 3 parallel samples.

[0067] Microbiological testing: The tests were conducted according to the current standard methods for detecting total bacterial count, Escherichia coli, and Salmonella.

[0068] Toxin detection: Aflatoxin B1 (AFB1) and vomitoxin (DON) were detected by ELISA, and the procedure was performed according to the kit instructions.

[0069] (2) Records of production time and ingredient flexibility Record the total production time and temperature control deviation for three consecutive batches (each batch processing 3 tons of raw materials).

[0070] (3) Gas emission detection During the production process, gas samples were collected from the exhaust ports of the batching heating fermentation tank, biochemical reaction vessel, and circulating shear filtration system to detect the concentrations of NH3, H2S, CH4, and CO2.

[0071] 2. Feeding Application Experiment Group design: Control group basal diet (corn-soybean meal type); Experimental group 1: basal diet + 5% chicken manure-based liquid feed; Experimental group 2: basal diet + 5% waste fish and shrimp-based liquid feed.

[0072] The feeding and management followed the original plan of the conventional feeding site, with a total experimental period of 28 days. Feed and water were provided freely, and daily feed intake was recorded. On the 7th day of the experiment, a dual-trough preference test (basal diet vs. diet containing 5% liquid feed) was conducted, and feed intake within 1 hour was recorded.

[0073] III. Experimental Results (a) Safety test results Table 1. Results of microbial and toxin detection (n=3)

[0074] Table 2 Production Duration Record (n=3 batches)

[0075] Table 3. Gas emission test results (ppm, n=3)

[0076] (II) Feeding Application Results Table 4. Feed intake preference test of piglets (1-hour feed intake, g, n=20)

[0077] Table 5. Weight and feed intake of piglets at 28 days of age (n=20)

[0078] The results above show that microbial and toxin detection indicated that no pathogenic microorganisms were detected in either liquid feed, and toxin residues were far below national standards (Table 1), proving that the process of this invention can effectively inactivate pathogens and degrade toxins. The total production time of this invention can be controlled within 6 hours (Table 2), with high temperature control accuracy and no impact on equipment stability from feed ratio adjustments, indicating that the production system is efficient and flexible. Gas emission detection showed extremely low concentrations of odorous gases such as NH3 and H2S (Table 3), meeting green production requirements. Preference experiments showed that the liquid feed significantly improved feed intake (Table 4), and the final weight and feed conversion ratio of piglets in the feeding experimental group were better than those in the control group (Table 5), proving that the product is easily digestible and absorbable. The small-molecule liquid feed produced by this invention has high raw material safety, high production efficiency and environmental friendliness, and good palatability, possessing good application value.

[0079] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A method for producing a small-molecule liquid biological feed ingredient, characterized in that, Includes the following steps: S1. The biomass raw material is subjected to impurity removal and activation pulping treatment to obtain activated pulp; S2. The activated slurry is subjected to heating and fermentation treatment to obtain fermented slurry; S3. The fermented slurry is subjected to a biochemical reaction treatment to obtain a biochemical slurry; S4. The biochemical slurry is subjected to a closed-loop pressure cyclic shear reaction treatment, and filtered to obtain a small molecule filtrate and residual solids. The small molecule filtrate is the small molecule liquid biological feed raw material.

2. The production method according to claim 1, characterized in that, The biomass raw materials include one or more of the following: crop straw, forage, grain chaff, brewing lees, soy sauce residue, vinegar residue, sugar residue, fruit residue, soybean residue, rapeseed, peanut, cottonseed, oil residue, kitchen waste, livestock and poultry manure, aquatic product processing by-products, fish and shrimp waste, fly larvae and insects, algae, food processing waste, fruit and vegetable processing residues, forestry processing by-products, and traditional Chinese medicine residues.

3. The production method according to claim 1, characterized in that, In S1, the activated pulping treatment is a mechanical pulping treatment; The rotation speed of the mechanical pulping process is 100~1200 rpm; The mechanical pulping process takes 30-60 minutes. In S1, during the mechanical pulping process, the dry matter concentration of the liquid is controlled to be 10%~30%.

4. The production method according to claim 1, characterized in that, In S2, the heating and fermentation process includes: First stage of preheating: The activated slurry is heated to 65~75℃ and kept at that temperature for 20~40 minutes; The second stage is cooling and fermentation: the pretreated material is cooled to 30~35℃ and a fermentation agent is added to carry out the fermentation reaction.

5. The production method according to claim 4, characterized in that, The fermentation agent includes Bacillus licheniformis preparation and Saccharomyces cerevisiae powder; The fermentation reaction is carried out at a temperature of 30~35℃; The fermentation reaction takes 30 to 120 minutes.

6. The production method according to claim 1, characterized in that, In S3, the biochemical reaction process is a catalytic hydrolysis reaction carried out under acidic conditions; The pH value of the catalytic hydrolysis reaction is 2.5~4.0; The temperature of the catalytic hydrolysis reaction is 80~140℃; The catalytic hydrolysis reaction takes 2 to 6 hours.

7. The production method according to claim 1, characterized in that, In S4, the closed-loop shear reaction filtration process is achieved in a closed loop consisting of a high-speed shear disperser and a filter; The operating pressure of the closed-loop circuit is 0.4~1MPa; The high-shear disperser operates at a speed of 3000~12000 rpm; The filter has a filtration precision of 100~300 mesh; In S4, the solids obtained from filtration are returned to the biochemical reaction treatment step in S3 to participate in the reaction again or to undergo environmental protection treatment. The production method further includes step S5: concentrating the small molecule filtrate obtained in S4 to obtain a concentrated liquid with a solid content of 25% to 50%.

8. The production method according to claim 1, characterized in that, In one or more steps S1, S2, S3, and S4, sensors are set up for real-time monitoring, and the processing parameters of the corresponding steps are adjusted according to the monitoring results. The monitoring includes monitoring one or more of the following: slurry moisture content, particle size, amount of prepared materials added, pH value, temperature, pressure, shear force, rotation speed, filtration rate, energy consumption, weight, and time.

9. The production method according to claim 1, characterized in that, A pressure buffer tank is installed in the biochemical reaction treatment of S3 and / or the closed-loop reaction treatment of S4 to regulate the pressure fluctuations generated during the reaction process. And / or, steps S1, S2, S3, and S4 are connected by one or more of the following methods: pipes, pumps, valves, and screw conveyors, to achieve continuous production; And / or, a built-in and / or external heating pump is installed in the slurry container, wherein the heater is one or a combination of several of the following: a resistance heater, an electromagnetic heater, or an external heat pump heating device, for energy-saving heating of the material.

10. Small molecule liquid biological feed raw material produced by the production method according to any one of claims 1 to 9.

Images