Preparation method of ganoderma lucidum mycelium hair nourishing emulsion
By fermenting Ganoderma lucidum mycelium and seafood mushroom mycelium, and using soybean processing by-products to prepare Ganoderma lucidum mycelium hair nourishing milk, the problems of side effects of chemical components and low utilization rate of by-products are solved, achieving safe and effective hair nourishing and hair growth effects and high-value utilization of resources.
Patent Information
- Application Number
- CN202511411370.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-09-29
- Publication Date
- 2025-12-12
AI Technical Summary
Existing hair growth and health products mostly rely on chemically synthesized ingredients, which have side effects, and the utilization rate of soybean processing by-products is low, resulting in insufficient economic added value.
Ganoderma lucidum mycelium and seafood mushroom mycelium are fermented, and soybean residue, a by-product of soybean processing, is used as a culture medium to prepare Ganoderma lucidum mycelium hair nourishing milk through microbial fermentation. Active ingredients are extracted by combining ethanol and glycerin, and cosmetic-grade raw materials are added to prepare the hair nourishing milk.
We provide safe, efficient, and green hair growth products that promote blood circulation in hair follicles, have anti-inflammatory and antioxidant effects, solve the problem of low utilization rate of soybean processing by-products, and realize the high-value utilization of agricultural solid waste.
Smart Images
Figure CN121102071A_ABST
Abstract
Description
Technical Field
[0001] This invention belongs to the field of microbial fermentation in the field of biotechnology, and specifically relates to a method for preparing Ganoderma lucidum mycelium hair nourishing milk. Background Technology
[0002] In recent years, with increasing life stress and environmental factors, hair loss has become increasingly common, leading to a significant increase in consumer demand for natural and safe hair growth products. Currently, the development of hair growth products is gradually shifting from chemically synthesized ingredients to plant-derived active ingredients, aiming to reduce side effects and improve user compliance. Commonly used plants for hair growth include Polygonum multiflorum, Platycladus orientalis leaves, ginger, sesame seeds, and rosemary. Their active ingredients, such as flavonoids, saponins, polysaccharides, and volatile oils, are believed to promote blood circulation in hair follicles, have anti-inflammatory, antioxidant, and nourishing effects on hair follicles.
[0003] The preparation of safe, efficient, and environmentally friendly hair growth emulsions using microbial ferments or their extracts as raw materials has attracted widespread attention. For example, fermentation products from red quinoa and shiitake mushrooms can produce abundant polysaccharides, amino acids, small molecule peptides, vitamins, and organic acids, which have excellent antioxidant, anti-inflammatory, and moisturizing properties. They can effectively improve the scalp microenvironment, reduce inflammation around hair follicles, and create healthy conditions for hair growth.
[0004] In traditional Chinese medicine, Ganoderma lucidum (Reishi mushroom) has long been recognized as a precious and valuable herb, possessing physiological functions such as protecting against damage from radiotherapy and chemotherapy, sedation, antidepression, protection against cerebral ischemia, regulation of blood lipids, blood pressure, and blood sugar, and liver and kidney protection. These functional activities of Ganoderma lucidum are related to its various nutrients and active substances, such as proteins, amino acids, polysaccharides, polypeptides, triterpenes, taurine, adenosine, alkaloids, and volatile oils. Among these, the two most important active substances are triterpenes and polysaccharides. Triterpenes and polysaccharides also have hair-growth-promoting effects. Triterpenes can improve microcirculation, stimulate the dilation of capillaries in the dermal papilla, increase blood flow, and deliver more oxygen and nutrients to the hair follicles; inhibit inflammatory factors in the scalp, alleviating hair loss caused by inflammation around the hair follicles; and prolong the hair follicle growth phase and activate hair follicles in the resting phase by regulating key signaling pathways such as Wnt / β-catenin and TGF-β. The hair growth and health benefits of polysaccharides are mainly reflected in the following aspects: forming a moisturizing film on the scalp to reduce moisture loss; scavenging free radicals and protecting hair follicle cells from oxidative stress damage; serving as an energy source for beneficial microorganisms in the skin and maintaining the health of the scalp's microecology; providing support for rapidly dividing hair follicle cells; and promoting the proliferation of dermal papilla cells and enhancing their activity, thereby supporting hair shaft growth.
[0005] Soybean dregs and soybean meal are byproducts of soybean processing. Soybean dregs are the residue filtered out after making tofu or other soy products, while soybean meal is the solid waste after soybean oil extraction. As byproducts of soybean processing, their utilization rate and economic added value are both low. Producing functional active substances through microbial fermentation is one of the effective ways to utilize soybean processing byproducts to a higher value. Summary of the Invention
[0006] To address the aforementioned problems, this invention provides a method for preparing Ganoderma lucidum mycelium hair growth lotion. One objective is to provide Ganoderma lucidum mycelium hair growth lotion and its preparation method, offering a safe, efficient, and green hair growth product to reduce the side effects of using chemical shampoos. Another objective is to solve the problems of poor utilization rate and low economic added value of soybean processing by-products.
[0007] A method for preparing a Ganoderma lucidum mycelium hair growth lotion includes the following steps:
[0008] I. Preparation of Ganoderma lucidum fermentation seed liquid:
[0009] Ganoderma lucidum inoculum is inoculated into Ganoderma lucidum seed liquid culture medium and cultured for a period of time to obtain Ganoderma lucidum fermented seed liquid;
[0010] The Ganoderma strain mentioned in step one is Ganoderma lucidum;
[0011] II. Preparation of seafood mushroom mycelium mixture:
[0012] Seafood mushroom spawn is inoculated into PDA liquid fermentation medium and cultured for a period of time to obtain seafood mushroom fermentation seed liquid; seafood mushroom fermentation seed liquid is inoculated into seafood mushroom solid fermentation medium, and the mixture of medium and mycelium obtained after solid fermentation is seafood mushroom mycelium mixture.
[0013] III. Preparation of Ganoderma lucidum solid-state fermentation culture medium:
[0014] The solid fermentation culture medium for Ganoderma lucidum has a water content of 48-55% and includes, by weight: 50-100 parts soybean residue, 35-50 parts seafood mushroom mycelium mixture, and 0.1-0.3 parts vitamin B12;
[0015] IV. Inoculation and fermentation of Ganoderma lucidum seed liquid:
[0016] The Ganoderma lucidum fermentation seed liquid was inoculated into the Ganoderma lucidum solid fermentation medium, stirred and mixed evenly, cultured for a period of time, crushed, and sieved to obtain Ganoderma lucidum fermentation mycelium powder.
[0017] V. Extraction of active ingredients from Ganoderma lucidum fermentation mycelium:
[0018] First, the fermented mycelium powder of Ganoderma lucidum was ultrasonically extracted in an ethanol-water solution, and the solid and liquid were separated to obtain extract A and the residue after extraction. The residue after extraction was stirred and extracted in an glycerol-water solution, and the solid and liquid were separated to obtain extract B. Extract A and extract B were combined and concentrated by vacuum distillation to obtain extract of Ganoderma lucidum active ingredients.
[0019] VI. Preparation of Ganoderma lucidum mycelium hair nourishing emulsion:
[0020] ① Weigh out 85-88 parts of deionized water, 5-8 parts of Ganoderma lucidum active ingredient extract, 1-2 parts of cetearyl alcohol, 1-2 parts of behenyltrimethylammonium methyl sulfate, 1-1.5 parts of glycerin, 0.5-0.8 parts of phenoxyethanol and 0.1 parts of plant essential oil by weight;
[0021] ② Under stirring conditions, the weighed raw materials are mixed to obtain Ganoderma lucidum mycelium hair nourishing milk.
[0022] The Ganoderma lucidum strains and seafood mushroom strains used in this invention are both commercially available products.
[0023] The method of using the Ganoderma lucidum mycelium hair nourishing lotion in this invention is as follows: First, wash your hair thoroughly with a suitable shampoo, then rinse it thoroughly with warm water. Next, use a dry towel to absorb most of the water, leaving the hair slightly damp. Apply the Ganoderma lucidum mycelium hair nourishing lotion evenly to the scalp or hair roots, and gently massage the scalp with your fingers for 5-10 minutes to facilitate the absorption of active substances. After use, air dry your hair or use a paper towel to absorb the moisture.
[0024] The ingredients in the Ganoderma lucidum mycelium hair nourishing lotion prepared by this invention are all food-grade or cosmetic-grade.
[0025] Advantages of this invention:
[0026] I. This invention first utilizes soybeans and their processing by-products as the main raw material for fermentation. The fermentation raw materials are natural, readily available, inexpensive, and easy to process. The fermentation conditions are mild and efficient, and the fermentation process has the advantages of producing no waste and being environmentally friendly. The Ganoderma lucidum active ingredient extract obtained after fermentation has the characteristics of promoting blood circulation in hair follicles, anti-inflammatory, antioxidant, and nourishing hair follicles. The Ganoderma lucidum mycelium hair nourishing milk has the effects of nourishing and protecting hair, and promoting hair growth.
[0027] Second, the fermentation method and preparation method provided by this invention offer an effective way to utilize agricultural solid waste for high value and enrich Ganoderma lucidum products, with very broad application prospects. Attached Figure Description
[0028] Figure 1 A digital photograph of the Ganoderma lucidum mycelium hair growth lotion prepared in Example 1;
[0029] Figure 2The Ganoderma lucidum mycelium hair growth lotion prepared in Example 1 is shown in the bactericidal curve of Propionibacterium acnes at the MIC concentration.
[0030] Figure 3 The image shows the bactericidal curve of the Ganoderma lucidum mycelium hair growth lotion prepared in Example 1 against Staphylococcus aureus according to the MIC concentration. Detailed Implementation
[0031] Specific Implementation Method 1: This implementation method provides a method for preparing Ganoderma lucidum mycelium hair nourishing lotion, comprising the following steps:
[0032] I. Preparation of Ganoderma lucidum fermentation seed liquid:
[0033] Ganoderma lucidum inoculum is inoculated into Ganoderma lucidum seed liquid culture medium and cultured for a period of time to obtain Ganoderma lucidum fermented seed liquid;
[0034] The Ganoderma strain mentioned in step one is Ganoderma lucidum;
[0035] II. Preparation of seafood mushroom mycelium mixture:
[0036] Seafood mushroom spawn is inoculated into PDA liquid fermentation medium and cultured for a period of time to obtain seafood mushroom fermentation seed liquid; seafood mushroom fermentation seed liquid is inoculated into seafood mushroom solid fermentation medium, and the mixture of medium and mycelium obtained after solid fermentation is seafood mushroom mycelium mixture.
[0037] III. Preparation of Ganoderma lucidum solid-state fermentation culture medium:
[0038] The solid fermentation culture medium for Ganoderma lucidum has a water content of 48-55% and includes, by weight: 50-100 parts soybean residue, 35-50 parts seafood mushroom mycelium mixture, and 0.1-0.3 parts vitamin B12;
[0039] IV. Inoculation and fermentation of Ganoderma lucidum seed liquid:
[0040] The Ganoderma lucidum fermentation seed liquid was inoculated into the Ganoderma lucidum solid fermentation medium, stirred and mixed evenly, cultured for a period of time, crushed, and sieved to obtain Ganoderma lucidum fermentation mycelium powder.
[0041] V. Extraction of active ingredients from Ganoderma lucidum fermentation mycelium:
[0042] First, the fermented mycelium powder of Ganoderma lucidum was ultrasonically extracted in an ethanol-water solution, and the solid and liquid were separated to obtain extract A and the residue after extraction. The residue after extraction was stirred and extracted in an glycerol-water solution, and the solid and liquid were separated to obtain extract B. Extract A and extract B were combined and concentrated by vacuum distillation to obtain extract of Ganoderma lucidum active ingredients.
[0043] VI. Preparation of Ganoderma lucidum mycelium hair nourishing emulsion:
[0044] ① Weigh out 85-88 parts of deionized water, 5-8 parts of Ganoderma lucidum active ingredient extract, 1-2 parts of cetearyl alcohol, 1-2 parts of behenyltrimethylammonium methyl sulfate, 1-1.5 parts of glycerin, 0.5-0.8 parts of phenoxyethanol and 0.1 parts of plant essential oil by weight;
[0045] ② Under stirring conditions, the weighed raw materials are mixed to obtain Ganoderma lucidum mycelium hair nourishing milk.
[0046] Specific Implementation Method Two: This implementation method differs from Specific Implementation Method One in that the preparation method of the Ganoderma lucidum seed liquid culture medium in step one is as follows: Soybean milk with a concentration of 12-25 g / L is sterilized at 121°C for 20 minutes and then cooled to room temperature to obtain the Ganoderma lucidum seed liquid culture medium. The soybean milk is prepared by mixing soybean powder (processed from soybeans through soaking, grinding, and spray drying) with water to obtain the 12-25 g / L soybean milk, or by processing soybeans through soaking, grinding with water, and filtering to obtain the 12-25 g / L soybean milk. Other steps are the same as in Specific Implementation Method One.
[0047] Specific Implementation Method Three: This implementation method differs from Specific Implementation Method One or Two in that: In step one, the Ganoderma lucidum spores are inoculated into the Ganoderma lucidum seed culture medium and cultured at 24-28℃ and 150-200 rpm for 5-7 days. After the culture is completed, sterile glass beads are added, and the mixture is shaken at 200 rpm for 20 minutes to break up the mycelial balls, thus obtaining the Ganoderma lucidum fermentation seed culture. The other steps are the same as in Specific Implementation Method One or Two.
[0048] Specific Implementation Method Four: This implementation method differs from Specific Implementation Methods One to Three in that: the preparation method of the seafood mushroom solid-state fermentation culture medium in step two is as follows: ① Mix soybean hulls, soybean meal, crushed corn cobs, rice bran, and potato peel residue in a weight ratio of 1:1:(0.2-0.3):0.1:0.1, adjust the moisture content to 55-68%, and use a bag-type fermentation method, filling each bag with 800-1000g, sterilizing, and cooling to room temperature to obtain the seafood mushroom solid-state fermentation culture medium; the crushed corn cobs are the cobs of corn or fresh corn after threshing, obtained by crushing the middle part remaining after threshing corn or fresh corn in a clean environment, with a moisture content of 11-13%. Other steps are the same as in Specific Implementation Methods One to Three.
[0049] Soybean (scientific name: Glycine max (Linn.) Merr.), commonly known as soybean, is the seed of soybean, an annual herbaceous plant belonging to the genus Glycine in the family Leguminosae.
[0050] The soybean skin mentioned in this embodiment is the outer seed coat of soybean.
[0051] The soybean meal described in this embodiment is a byproduct obtained after extracting oil from soybeans.
[0052] The rice bran described in this embodiment is fresh rice bran produced during the rice processing process from brown rice to rice.
[0053] The potato peel residue described in this embodiment is the waste peel residue generated during the peeling process in potato processing.
[0054] In this embodiment, the corn cobs are selected to remove impurities and moldy or deteriorated individuals, and then crushed using a straw crusher to obtain the corn cob fragments.
[0055] Specific Implementation Method Five: This implementation method differs from Specific Implementation Methods One to Four in that: in step two, the *Mushroom confermentation* fermentation seed liquid is inoculated into the *Mushroom confermentation* solid fermentation medium, and then cultured for 6-10 days under conditions of darkness, temperature of 24-30℃, and air humidity of 70-80%, to obtain a *Mushroom confermentation* mycelium mixture; the inoculation amount of the *Mushroom confermentation* fermentation seed liquid accounts for 5-10% of the weight of the *Mushroom confermentation* solid fermentation medium. Other steps are the same as in Specific Implementation Methods One to Four.
[0056] Preferably, in step two of this embodiment, the seafood mushroom fermentation seed liquid is inoculated into the seafood mushroom solid fermentation medium, and then cultured for 8-10 days under conditions of darkness, temperature of 26-28℃, and air humidity of 73-76% to obtain a seafood mushroom mycelium mixture.
[0057] Specific Implementation Method Six: This implementation method differs from Specific Implementation Methods One to Five in that: the preparation method of the seafood mushroom fermentation seed liquid in step two is as follows: Seafood mushroom spawn is inoculated into PDA liquid fermentation medium and cultured at 23-26℃ and 150-200 rpm for 5-7 days. After culture, sterile glass beads are added, and the mixture is shaken at 200 rpm for 20 minutes to break up the mycelial balls, thus obtaining the seafood mushroom fermentation seed liquid. The preparation method of the PDA liquid fermentation medium is as follows: 200g of peeled potatoes are weighed, 1000mL of water is added, and the mixture is boiled for 30 minutes. After filtration, 20g of glucose is added to make up the total volume to 1000mL, and the mixture is sterilized. Other steps are the same as in Specific Implementation Methods One to Five.
[0058] Specific Implementation Method Seven: This implementation method differs from Specific Implementation Methods One to Six in that: the preparation method of the Ganoderma lucidum solid-state fermentation culture medium in step three is as follows: 0.1-0.3 parts of vitamin B12 are dissolved in 10 times its weight of water, and stirred until homogeneous to obtain a vitamin B12 solution; 35-50 parts of seafood mushroom mycelium mixture and 50-100 parts of soybean residue are thoroughly stirred until homogeneous, then the vitamin B12 solution is added, stirred until homogeneous, and the moisture content is adjusted to 48-55%, then sterilized to obtain the Ganoderma lucidum solid-state fermentation culture medium; the soybean residue is the residue filtered after making tofu or other soy products from soybeans, which is then dried; the vitamin B12 is food grade. Other steps are the same as in Specific Implementation Methods One to Six.
[0059] Specific Implementation Method Eight: This implementation method differs from Specific Implementation Methods One to Seven in that: in step four, the Ganoderma lucidum fermentation seed liquid inoculated accounts for 5-10% of the weight of the Ganoderma lucidum solid fermentation culture medium; in step four, the Ganoderma lucidum fermentation seed liquid is inoculated into the Ganoderma lucidum solid fermentation culture medium, stirred and mixed evenly, and cultured in the dark for 6-7 days at 22-28℃ and air humidity of 60-70%. The dark conditions are then changed to indoor diffused light, and the culture continues for another 8-15 days. The obtained fermentation culture is then dried, pulverized, and passed through a 40-mesh sieve to obtain Ganoderma lucidum fermentation mycelium; the drying process is freeze-drying or low-temperature blow-drying, with the low-temperature blow-drying temperature not exceeding 45℃. Other steps are the same as in Specific Implementation Methods One to Seven.
[0060] Specific Implementation Method Nine: This implementation method differs from Specific Implementation Methods One to Eight in that: In step five, the Ganoderma lucidum fermented mycelium powder is added to 15-30 times its weight in a 75%-90% ethanol aqueous solution, thoroughly mixed, and then ultrasonically extracted for 15-25 minutes at 120-180W and 45-65℃. Solid-liquid separation is then performed to obtain extract A and the residue after extraction. The residue is added to 10-20 times its weight in a 25%-35% glycerol aqueous solution, stirred thoroughly, and extracted at 45-65℃ and 200-300rpm for 45-60 minutes. Solid-liquid separation is then performed to obtain extract B. Extracts A and B are combined and concentrated under reduced pressure at 35-55℃ to 40-50% of the initial weight, yielding the Ganoderma lucidum active ingredient extract. Other steps are the same as in Specific Implementation Methods One to Eight.
[0061] Specific Implementation Method Ten: This implementation method differs from Specific Implementation Methods One to Nine in that: the plant essential oil mentioned in step Six① is at least one of lavender essential oil, sandalwood essential oil, bergamot essential oil, and rosemary essential oil; in step Six②: under stirring conditions of 200-500 rpm, 1-1.5 parts of glycerin and 0.1 parts of plant essential oil are mixed evenly, then 1-2 parts of cetearyl alcohol are added and mixed evenly, then 0.5-0.8 parts of phenoxyethanol are added and mixed evenly, then 1-2 parts of behenyltrimethylammonium methyl sulfate are added, and finally 5-8 parts of Ganoderma lucidum active ingredient extract and 85-88 parts of deionized water are added, homogenized, and packaged to obtain Ganoderma lucidum mycelium hair nourishing lotion. Other steps are the same as in Specific Implementation Methods One to Nine.
[0062] The beneficial effects of the present invention are verified using the following embodiments:
[0063] Example 1: A method for preparing a Ganoderma lucidum mycelium hair growth lotion, comprising the following steps:
[0064] I. Preparation of Ganoderma lucidum fermentation seed liquid:
[0065] A piece of Ganoderma lucidum slant culture stored at 4℃ was inoculated into Ganoderma lucidum seed culture medium and cultured at 28℃ and 200rpm for 5 days. After the culture was completed, 8 sterile glass beads were added to the Erlenmeyer flask and shaken at 200rpm for 20 minutes to break up the mycelial balls, thus obtaining Ganoderma lucidum fermentation seed culture.
[0066] The Ganoderma strain mentioned in step one is Ganoderma lucidum;
[0067] The preparation method of Ganoderma lucidum seed liquid culture medium in step one is as follows: use commercially available soybean powder and water to prepare soybean milk with a concentration of 12g / L, natural pH, put 30mL into a 250mL Erlenmeyer flask, sterilize at 121℃ for 20min and then cool to room temperature to obtain Ganoderma lucidum seed liquid culture medium.
[0068] The commercially available soybean powder contains only soybean powder and no other substances.
[0069] II. Preparation of seafood mushroom mycelium mixture:
[0070] A piece of *Varicaria slant* spawn stored at 4℃ was inoculated into PDA liquid fermentation medium and cultured at 26℃ and 200 rpm for 5 days. After culture, 8 sterile glass beads were added, and the mixture was shaken at 200 rpm for 20 minutes to break up the mycelial balls, obtaining *Varicaria slant* fermentation seed liquid. The *Varicaria slant* fermentation seed liquid was inoculated into *Varicaria slant* solid fermentation medium and cultured for 6 days in the dark at 30℃ and 70% humidity to obtain *Varicaria slant* mycelial mixture. The inoculation amount of the *Varicaria slant* fermentation seed liquid accounted for 10% of the weight of the *Varicaria slant* solid fermentation medium.
[0071] The seafood mushroom strain mentioned in step two is scientifically named [Hypsizygus marmoreus (Peck) HEBigelow], also known as jade mushroom, spotted jade mushroom, or true pheasant mushroom;
[0072] The preparation method of PDA liquid fermentation medium in step two is as follows: weigh 200g of peeled potatoes, add 1000mL of water, boil for 30min, filter and take the filtrate, add 20g of glucose to make up the total volume to 1000mL, and sterilize to obtain the medium.
[0073] The preparation method of the seafood mushroom solid-state fermentation medium in step two is as follows: Soybean hulls, soybean meal, crushed corn cobs, rice bran, and potato peel residue are mixed in a weight ratio of 1:1:0.3:0.1:0.1, and the moisture content is adjusted to 55%. Fermentation is carried out using a bag-type fermentation method, with each bag filled with 1000g of the mixture. The mixture is then sterilized and cooled to room temperature to obtain the seafood mushroom solid-state fermentation medium. The crushed corn cobs are the cobs remaining after threshing corn or fresh corn in a clean environment, and are obtained by crushing the middle part of the corn or fresh corn after harvesting. The moisture content is 13%.
[0074] III. Preparation of Ganoderma lucidum solid-state fermentation culture medium:
[0075] The Ganoderma lucidum solid-state fermentation culture medium has a water content of 48% and comprises, by weight: 100 parts soybean residue, 50 parts seafood mushroom mycelium mixture, and 0.3 parts vitamin B12; the soybean residue is the residue filtered after making tofu or other soy products from soybeans, which is then dried; the vitamin B12 is food grade; the preparation method is as follows: dissolve 0.3 parts vitamin B12 in 10 times its weight of water and stir until homogeneous to obtain a vitamin B12 solution; thoroughly stir and mix 50 parts seafood mushroom mycelium mixture and 100 parts soybean residue, then add the vitamin B12 solution and stir until homogeneous, adjust the water content to 48%, allow the pH to remain at a natural level, sterilize, and obtain the Ganoderma lucidum solid-state fermentation culture medium;
[0076] IV. Inoculation and fermentation of Ganoderma lucidum seed liquid:
[0077] The Ganoderma lucidum fermentation seed liquid was inoculated into the Ganoderma lucidum solid fermentation medium, stirred and mixed evenly, and cultured in the dark for 6 days at 28℃ and 60% humidity. The dark conditions were then changed to indoor diffused light and cultured for another 15 days. The obtained fermentation culture was then dried, pulverized and passed through a 40-mesh sieve to obtain Ganoderma lucidum fermented mycelium powder. The drying process was freeze-drying.
[0078] In step four, the inoculation of Ganoderma lucidum fermentation seed liquid accounts for 10% of the weight of Ganoderma lucidum solid fermentation culture medium;
[0079] V. Extraction of active ingredients from Ganoderma lucidum fermentation mycelium:
[0080] One part by weight of Ganoderma lucidum fermented mycelium powder was added to 15 times its weight of an 85% ethanol aqueous solution, and the mixture was thoroughly mixed. Then, the mixture was ultrasonically extracted for 15 minutes at 180W and 45℃. Solid-liquid separation was performed to obtain extract A and the residue after extraction. The residue after extraction was added to 12 times its weight of a 25% glycerol aqueous solution, and the mixture was stirred and extracted for 60 minutes at 45℃ and 200rpm. Solid-liquid separation was performed to obtain extract B. Extracts A and B were combined and concentrated under reduced pressure at 45℃ to 50% of the initial weight to obtain the Ganoderma lucidum active ingredient extract.
[0081] VI. Preparation of Ganoderma lucidum mycelium hair nourishing emulsion:
[0082] ① Weigh out 85 parts deionized water, 8 parts Ganoderma lucidum active ingredient extract, 2 parts cetearyl alcohol, 2 parts behenyltrimethylammonium methyl sulfate, 1 part glycerin, 0.5 parts phenoxyethanol and 0.1 parts plant essential oil by weight;
[0083] The plant essential oil mentioned in step six① is rosemary essential oil;
[0084] ② Under stirring conditions of 200 rpm, mix 1 part glycerin and 0.1 part plant essential oil evenly, then add 2 parts cetearyl alcohol and mix evenly, then add 0.5 parts phenoxyethanol and mix evenly, then add 2 parts behenyltrimethylammonium methyl sulfate, and finally add 8 parts Ganoderma lucidum active ingredient extract and 85 parts deionized water, homogenize, and package to obtain Ganoderma lucidum mycelium hair nourishing milk.
[0085] Cetearyl alcohol, behenyltrimethylammonium methyl sulfate, phenoxyethanol, glycerin, and rosemary essential oil are all food grade.
[0086] The method of using Ganoderma lucidum mycelium hair nourishing lotion is as follows: First, wash your hair with shampoo, then use a dry towel to absorb most of the water, leaving your hair slightly damp. Apply the Ganoderma lucidum mycelium hair nourishing lotion evenly to the scalp or hair roots, and gently massage the scalp with your fingers for 5 minutes to facilitate the absorption of active substances. After use, let your hair air dry.
[0087] Example 2: A method for preparing a Ganoderma lucidum mycelium hair growth lotion, comprising the following steps:
[0088] I. Preparation of Ganoderma lucidum fermentation seed liquid:
[0089] A piece of Ganoderma lucidum slant culture stored at 4℃ was inoculated into Ganoderma lucidum seed culture medium and cultured at 24℃ and 150rpm for 7 days. After the culture was completed, 8 sterile glass beads were added to the Erlenmeyer flask and shaken at 200rpm for 20 minutes to break up the mycelial balls, thus obtaining Ganoderma lucidum fermentation seed culture.
[0090] The Ganoderma strain mentioned in step one is Ganoderma lucidum;
[0091] The preparation method of Ganoderma lucidum seed liquid culture medium in step one is as follows: Soybean powder processed by soybeans through processes including but not limited to soaking, grinding and spray drying is used to prepare soybean milk with a concentration of 25g / L, natural pH, 30mL in a 250mL Erlenmeyer flask, sterilized at 121℃ for 20min and then cooled to room temperature to obtain Ganoderma lucidum seed liquid culture medium.
[0092] II. Preparation of seafood mushroom mycelium mixture:
[0093] A piece of *Varicaria slant* spawn stored at 4℃ was inoculated into PDA liquid fermentation medium and cultured at 23℃ and 150 rpm for 7 days. After culture, 8 sterile glass beads were added, and the mixture was shaken at 200 rpm for 20 minutes to break up the mycelial balls, obtaining *Varicaria slant* fermentation seed liquid. The *Varicaria slant* fermentation seed liquid was inoculated into *Varicaria slant* solid fermentation medium and cultured for 7 days in the dark at 25℃ and 75% humidity to obtain *Varicaria slant* mycelial mixture. The inoculation amount of the *Varicaria slant* fermentation seed liquid accounted for 5% of the weight of the *Varicaria slant* solid fermentation medium.
[0094] The seafood mushroom strain mentioned in step two is scientifically named [Hypsizygus marmoreus (Peck) HEBigelow], also known as jade mushroom, spotted jade mushroom, or true pheasant mushroom;
[0095] The preparation method of PDA liquid fermentation medium in step two is as follows: weigh 200g of peeled potatoes, add 1000mL of water, boil for 30min, filter and take the filtrate, add 20g of glucose to make up the total volume to 1000mL, and sterilize to obtain the medium.
[0096] The preparation method of the seafood mushroom solid-state fermentation medium in step two is as follows: Soybean hulls, soybean meal, crushed corn cobs, rice bran, and potato peel residue are mixed in a weight ratio of 1:1:0.2:0.1:0.1, and the moisture content is adjusted to 55%. Fermentation is carried out using a bag-type fermentation method, with each bag filled with 800g of the mixture. The mixture is then sterilized and cooled to room temperature to obtain the seafood mushroom solid-state fermentation medium. The crushed corn cobs are the cobs remaining after threshing corn or fresh corn in a clean environment, and are obtained by crushing the middle part of the corn or fresh corn after harvesting. The moisture content is 11%.
[0097] III. Preparation of Ganoderma lucidum solid-state fermentation culture medium:
[0098] The Ganoderma lucidum solid-state fermentation culture medium has a water content of 50% and comprises, by weight: 50 parts soybean residue, 35 parts seafood mushroom mycelium mixture, and 0.1 parts vitamin B12; the soybean residue is the residue filtered after making tofu or other soy products from soybeans, which is then dried; the vitamin B12 is food grade; the preparation method is as follows: dissolve 0.1 parts vitamin B12 in 10 times its weight of water, stir and mix evenly to obtain a vitamin B12 solution; thoroughly stir and mix 35 parts seafood mushroom mycelium mixture and 50 parts soybean residue, then add the vitamin B12 solution and stir evenly, adjust the water content to 50%, allow the pH to remain natural, sterilize, and obtain the Ganoderma lucidum solid-state fermentation culture medium;
[0099] IV. Inoculation and fermentation of Ganoderma lucidum seed liquid:
[0100] The Ganoderma lucidum fermentation seed liquid was inoculated into the Ganoderma lucidum solid fermentation medium, stirred and mixed evenly, and cultured in the dark for 7 days at 24℃ and 70% humidity. The dark conditions were then changed to indoor diffused light and cultured for another 14 days. The obtained fermentation culture was then dried, pulverized and passed through a 40-mesh sieve to obtain Ganoderma lucidum fermentation mycelium powder. The drying process was carried out by blowing air at 45℃.
[0101] In step four, the inoculation of Ganoderma lucidum fermentation seed liquid accounts for 8% of the weight of the Ganoderma lucidum solid fermentation culture medium;
[0102] V. Extraction of active ingredients from Ganoderma lucidum fermentation mycelium:
[0103] One part by weight of Ganoderma lucidum fermented mycelium powder was added to 30 parts by weight of an 85% ethanol aqueous solution, and the mixture was thoroughly mixed. Then, the mixture was ultrasonically extracted at 180W and 65℃ for 25 minutes. Solid-liquid separation was performed to obtain extract A and the residue after extraction. The residue after extraction was added to 20 parts by weight of a 35% glycerol aqueous solution, and the mixture was stirred and extracted at 55℃ and 300rpm for 45 minutes. Solid-liquid separation was performed to obtain extract B. Extracts A and B were combined and concentrated under reduced pressure at 55℃ to 40% of the initial weight to obtain the Ganoderma lucidum active ingredient extract.
[0104] VI. Preparation of Ganoderma lucidum mycelium hair nourishing emulsion:
[0105] ① Weigh out 88 parts deionized water, 5 parts Ganoderma lucidum active ingredient extract, 1 part cetearyl alcohol, 1 part behenyltrimethylammonium methyl sulfate, 1 part glycerin, 0.5 parts phenoxyethanol and 0.1 parts plant essential oil by weight;
[0106] The plant essential oil mentioned in step six① is lavender essential oil;
[0107] ② Under stirring conditions of 500 rpm, mix 1 part glycerin and 0.1 part plant essential oil evenly, then add 1 part cetearyl alcohol and mix evenly, then add 0.5 part phenoxyethanol and mix evenly, then add 1 part behenyltrimethylammonium methyl sulfate, and finally add 5 parts Ganoderma lucidum active ingredient extract and 88 parts deionized water, homogenize, and package to obtain Ganoderma lucidum mycelium hair nourishing milk.
[0108] Cetearyl alcohol, behenyltrimethylammonium methyl sulfate, phenoxyethanol, glycerin, and lavender essential oil are all food grade.
[0109] Homogenization was performed using standard methods.
[0110] The method of using Ganoderma lucidum mycelium hair nourishing lotion is as follows: First, wash your hair with shampoo, then use a dry towel to absorb most of the water, leaving your hair slightly damp. Apply the Ganoderma lucidum mycelium hair nourishing lotion evenly to the scalp or hair roots, and gently massage the scalp with your fingers for 5 minutes to facilitate the absorption of active substances. After use, let your hair air dry.
[0111] Example 3: A method for preparing a Ganoderma lucidum mycelium hair growth lotion, comprising the following steps:
[0112] I. Preparation of Ganoderma lucidum fermentation seed liquid:
[0113] A piece of Ganoderma lucidum slant culture stored at 4℃ was inoculated into Ganoderma lucidum seed culture medium and cultured at 24℃ and 180rpm for 6 days. After the culture was completed, 8 sterile glass beads were added to the Erlenmeyer flask and shaken at 200rpm for 20 minutes to break up the mycelial balls, thus obtaining Ganoderma lucidum fermentation seed culture.
[0114] The Ganoderma strain mentioned in step one is Ganoderma lucidum;
[0115] The preparation method of Ganoderma lucidum seed liquid culture medium in step one is as follows: soybeans are processed through processes including but not limited to soaking, grinding with water, and filtering to obtain soybean milk with a concentration of 20g / L, natural pH, 30mL in a 250mL Erlenmeyer flask, sterilized at 121℃ for 20min and then cooled to room temperature to obtain Ganoderma lucidum seed liquid culture medium.
[0116] II. Preparation of seafood mushroom mycelium mixture:
[0117] A piece of *Varicaria slant* spawn stored at 4℃ was inoculated into PDA liquid fermentation medium and cultured at 25℃ and 180 rpm for 5 days. After culture, 8 sterile glass beads were added, and the mixture was shaken at 200 rpm for 20 minutes to break up the mycelial balls, obtaining *Varicaria slant* fermentation seed liquid. The *Varicaria slant* fermentation seed liquid was inoculated into *Varicaria slant* solid fermentation medium and cultured for 8 days in the dark at 26℃ and 75% humidity to obtain *Varicaria slant* mycelial mixture. The inoculation amount of the *Varicaria slant* fermentation seed liquid accounted for 8% of the weight of the *Varicaria slant* solid fermentation medium.
[0118] The seafood mushroom strain mentioned in step two is scientifically named [Hypsizygus marmoreus (Peck) HEBigelow], also known as jade mushroom, spotted jade mushroom, or true pheasant mushroom;
[0119] The preparation method of PDA liquid fermentation medium in step two is as follows: weigh 200g of peeled potatoes, add 1000mL of water, boil for 30min, filter and take the filtrate, add 20g of glucose to make up the total volume to 1000mL, and sterilize to obtain the medium.
[0120] The preparation method of the seafood mushroom solid-state fermentation medium in step two is as follows: Soybean hulls, soybean meal, crushed corn cobs, rice bran, and potato peel residue are mixed in a weight ratio of 1:1:0.25:0.1:0.1, and the moisture content is adjusted to 60%. Fermentation is carried out using a bag-type fermentation method, with each bag filled with 900g of the mixture. The mixture is then sterilized and cooled to room temperature to obtain the seafood mushroom solid-state fermentation medium. The crushed corn cobs are the cobs remaining after threshing corn or fresh corn in a clean environment, and are obtained by crushing the middle part of the corn or fresh corn after harvesting. The moisture content is 12%.
[0121] III. Preparation of Ganoderma lucidum solid-state fermentation culture medium:
[0122] The Ganoderma lucidum solid-state fermentation medium has a water content of 52% and comprises, by weight: 80 parts soybean residue, 35 parts seafood mushroom mycelium mixture, and 0.2 parts vitamin B12; the soybean residue is the residue filtered after making tofu or other soy products from soybeans, which is then dried; the vitamin B12 is food grade; the preparation method is as follows: dissolve 0.2 parts vitamin B12 in 10 times its weight of water, stir and mix evenly to obtain a vitamin B12 solution; thoroughly stir and mix 35 parts seafood mushroom mycelium mixture and 80 parts soybean residue, then add the vitamin B12 solution and stir evenly, adjust the water content to 52%, allow the pH to remain natural, sterilize, and obtain the Ganoderma lucidum solid-state fermentation medium;
[0123] IV. Inoculation and fermentation of Ganoderma lucidum seed liquid:
[0124] The Ganoderma lucidum fermentation seed liquid was inoculated into the Ganoderma lucidum solid fermentation medium, stirred and mixed evenly, and cultured in the dark for 7 days at 25℃ and 65% humidity. The dark conditions were then changed to indoor diffused light and cultured for another 13 days. The obtained fermentation culture was then dried, pulverized and passed through a 40-mesh sieve to obtain Ganoderma lucidum fermented mycelium powder. The drying process was freeze-drying.
[0125] In step four, the inoculation of Ganoderma lucidum fermentation seed liquid accounts for 8% of the weight of the Ganoderma lucidum solid fermentation culture medium;
[0126] V. Extraction of active ingredients from Ganoderma lucidum fermentation mycelium:
[0127] One part by weight of Ganoderma lucidum fermented mycelium powder was added to 20 parts by weight of an 80% ethanol aqueous solution, and the mixture was thoroughly mixed. Then, the mixture was ultrasonically extracted at 165W and 50℃ for 20 minutes. Solid-liquid separation was performed to obtain extract A and the residue after extraction. The residue after extraction was added to 15 parts by weight of a 30% glycerol aqueous solution, and the mixture was stirred and extracted at 50℃ and 200rpm for 50 minutes. Solid-liquid separation was performed to obtain extract B. Extracts A and B were combined and concentrated by vacuum distillation at 45℃ to 50% of the initial weight to obtain the Ganoderma lucidum active ingredient extract.
[0128] VI. Preparation of Ganoderma lucidum mycelium hair nourishing emulsion:
[0129] ① Weigh out 86 parts deionized water, 7 parts Ganoderma lucidum active ingredient extract, 2 parts cetearyl alcohol, 2 parts behenyltrimethylammonium methyl sulfate, 1 part glycerin, 0.6 parts phenoxyethanol and 0.1 parts plant essential oil by weight;
[0130] The plant essential oil mentioned in step six① is sandalwood essential oil;
[0131] ② Under stirring conditions of 500 rpm, mix 1 part glycerin and 0.1 part plant essential oil evenly, then add 2 parts cetearyl alcohol and mix evenly, then add 0.6 parts phenoxyethanol and mix evenly, then add 2 parts behenyltrimethylammonium methyl sulfate, and finally add 7 parts Ganoderma lucidum active ingredient extract and 86 parts deionized water, homogenize, and package to obtain Ganoderma lucidum mycelium hair nourishing milk.
[0132] The method of using Ganoderma lucidum mycelium hair nourishing lotion is as follows: First, wash your hair with shampoo, then use a dry towel to absorb most of the water, leaving your hair slightly damp. Apply the Ganoderma lucidum mycelium hair nourishing lotion evenly to the scalp or hair roots, and gently massage the scalp with your fingers for 7 minutes to facilitate the absorption of active substances. After use, let your hair air dry.
[0133] Hair growth lotion antibacterial effect test:
[0134] The sample tested was the Ganoderma lucidum mycelium hair growth lotion prepared in Example 1. The prepared Ganoderma lucidum mycelium hair growth lotion was as follows: Figure 1 As shown; the tested bacterial species were Propionibacterium acnes and Staphylococcus aureus, both of which are major bacteria that cause dermatitis, folliculitis and other problems. The minimum inhibitory concentration (MIC) and bactericidal curve of Ganoderma lucidum mycelium hair growth lotion against the two strains were determined.
[0135] MIC determination: The MIC test was conducted using a two-fold serial dilution method. The tested concentrations of the Ganoderma lucidum mycelium hair growth emulsion prepared in Example 1 were 8.64, 6.48, 5.40, 4.32, 3.24, 2.70, 2.16, 1.62, 1.35, and 1.08 mg / mL. After incubation at 37 ℃ for 24 hours, colonies were observed. The plate with no colonies formed in the highest dilution was the MIC of the Ganoderma lucidum mycelium hair growth emulsion. Based on the MIC test, the plates were incubated for another 24 hours at 37 ℃. The plate with no colonies formed in the highest dilution was the minimum bactericidal concentration (MBC) of the Ganoderma lucidum mycelium hair growth emulsion.
[0136] Bactericidal curve determination: The concentration of the two bacterial cultures, which had reached the logarithmic growth phase, was adjusted to 1×10⁻⁶ using their respective fresh liquid culture media. 6 CFU / mL, add the same volume of 1 / 4MIC, 1 / 2MIC, 1MIC, and 2MIC Ganoderma lucidum mycelium hair growth milk, with PBS as the control group, and culture in a shaker at 37°C for 8 hours. Take samples every 1 hour to determine the number of surviving bacteria using the plate method.
[0137] Staphylococcus aureus was cultured on LB medium at 37°C; Propionibacterium acnes was cultured on modified GAM medium at 37°C anaerobic. After sterilization of the modified GAM medium, 1 mL of sterile 0.1% vitamin K1 solution and 2 mg of sterile heme chloride were added to every 200 mL of the medium and mixed well; 2.5 g of agar was added to every 1000 mL of solid medium.
[0138] The MIC and MBC values of the Ganoderma lucidum mycelium hair growth lotion prepared in Example 1 are shown in Table 1.
[0139] Table 1. MIC and MBC of Ganoderma lucidum mycelium hair growth lotion
[0140]
[0141] Figure 2 The Ganoderma lucidum mycelium hair growth lotion prepared in Example 1 is shown in the bactericidal curve of Propionibacterium acnes at the MIC concentration.
[0142] Figure 3 The image shows the bactericidal curve of the Ganoderma lucidum mycelium hair growth lotion prepared in Example 1 against Staphylococcus aureus according to the MIC concentration.
[0143] The results show that the Ganoderma lucidum mycelium hair care lotion prepared in this application has a good antibacterial effect. With the extension of time, the Ganoderma lucidum mycelium hair care lotion showed a rapid bactericidal effect within 0-3 hours. After 3-4 hours, both bacteria achieved a high bactericidal effect at concentrations of 1MIC and 2MIC. When the concentration was lower than 1 MIC, it also had a good bactericidal effect.
[0144] The above description is only a partial embodiment of the present invention and is not intended to limit the present invention. The scope of patent protection of the present invention shall be determined by the claims. Similarly, any equivalent structural changes made based on the content of the present invention's specification shall also be included within the scope of protection of the present invention.
Claims
1. A method for preparing a Ganoderma lucidum mycelium hair growth lotion, characterized in that, Includes the following steps: I. Preparation of Ganoderma lucidum fermentation seed liquid: Ganoderma lucidum inoculum is inoculated into Ganoderma lucidum seed liquid culture medium and cultured for a period of time to obtain Ganoderma lucidum fermented seed liquid; The Ganoderma strain mentioned in step one is Ganoderma lucidum; II. Preparation of seafood mushroom mycelium mixture: Seafood mushroom spawn is inoculated into PDA liquid fermentation medium and cultured for a period of time to obtain seafood mushroom fermentation seed liquid; seafood mushroom fermentation seed liquid is inoculated into seafood mushroom solid fermentation medium, and the mixture of medium and mycelium obtained after solid fermentation is seafood mushroom mycelium mixture. III. Preparation of Ganoderma lucidum solid-state fermentation culture medium: The solid fermentation culture medium for Ganoderma lucidum has a water content of 48-55% and includes, by weight: 50-100 parts soybean residue, 35-50 parts seafood mushroom mycelium mixture, and 0.1-0.3 parts vitamin B12; IV. Inoculation and fermentation of Ganoderma lucidum seed liquid: The Ganoderma lucidum fermentation seed liquid was inoculated into the Ganoderma lucidum solid fermentation medium, stirred and mixed evenly, cultured for a period of time, crushed, and sieved to obtain Ganoderma lucidum fermentation mycelium powder. V. Extraction of active ingredients from Ganoderma lucidum fermentation mycelium: First, the fermented mycelium powder of Ganoderma lucidum was ultrasonically extracted in an ethanol-water solution, and the solid and liquid were separated to obtain extract A and the residue after extraction. The residue after extraction was stirred and extracted in an glycerol-water solution, and the solid and liquid were separated to obtain extract B. Extract A and extract B were combined and concentrated by vacuum distillation to obtain extract of Ganoderma lucidum active ingredients. VI. Preparation of Ganoderma lucidum mycelium hair nourishing emulsion: ① Weigh out 85-88 parts of deionized water, 5-8 parts of Ganoderma lucidum active ingredient extract, 1-2 parts of cetearyl alcohol, 1-2 parts of behenyltrimethylammonium methyl sulfate, 1-1.5 parts of glycerin, 0.5-0.8 parts of phenoxyethanol and 0.1 parts of plant essential oil by weight; ② Under stirring conditions, the weighed raw materials are mixed to obtain Ganoderma lucidum mycelium hair nourishing milk.
2. The method for preparing a Ganoderma lucidum mycelium hair nourishing emulsion according to claim 1, characterized in that, The preparation method of the Ganoderma lucidum seed liquid culture medium in step one is as follows: sterilize soybean milk with a concentration of 12-25 g / L at 121℃ for 20 min and then cool it to room temperature to obtain the Ganoderma lucidum seed liquid culture medium; the soybean milk is prepared by mixing soybean powder (made from soybeans through soaking, grinding, and spray drying) with water to obtain the soybean milk with a concentration of 12-25 g / L, or by processing soybeans through soaking, grinding with water, and filtering to obtain the soybean milk with a concentration of 12-25 g / L.
3. The method for preparing a Ganoderma lucidum mycelium hair nourishing emulsion according to claim 1, characterized in that, In step one, the Ganoderma lucidum spawn is inoculated into the Ganoderma lucidum seed culture medium and cultured at 24-28℃ and 150-200 rpm for 5-7 days. After the culture is completed, sterile glass beads are added and the mixture is shaken at 200 rpm for 20 minutes to break up the spawn, thus obtaining the Ganoderma lucidum fermentation seed culture.
4. The method for preparing a Ganoderma lucidum mycelium hair growth lotion according to claim 1, characterized in that, The preparation method of the seafood mushroom solid fermentation culture medium in step two is as follows: ① Mix soybean hulls, soybean meal, corn cob crumbs, rice bran, and potato peel residue in a weight ratio of 1:1:(0.2-0.3):0.1:0.1, adjust the moisture content to 55-68%, and use a bag fermentation method, filling each bag with 800-1000g, sterilizing, and cooling to room temperature to obtain the seafood mushroom solid fermentation culture medium; the corn cob crumbs are the cob of corn or fresh corn after threshing, which is the middle part left after threshing corn or fresh corn in a clean environment, and is obtained by crushing, with a moisture content of 11-13%.
5. The method for preparing a Ganoderma lucidum mycelium hair growth lotion according to claim 1, characterized in that, In step two, the seafood mushroom fermentation seed liquid is inoculated into the seafood mushroom solid fermentation medium, and then cultured for 6-10 days under conditions of darkness, temperature of 24-30℃, and air humidity of 70-80% to obtain a seafood mushroom mycelium mixture; the inoculation amount of the seafood mushroom fermentation seed liquid accounts for 5-10% of the weight of the seafood mushroom solid fermentation medium.
6. The method for preparing a Ganoderma lucidum mycelium hair nourishing emulsion according to claim 1, characterized in that, The preparation method of the seafood mushroom fermentation seed liquid in step two is as follows: inoculate the seafood mushroom spawn into PDA liquid fermentation medium, and culture it at 23-26℃ and 150-200rpm for 5-7 days. After the culture is completed, add sterile glass beads and shake at 200rpm for 20 minutes to break up the mycelial balls to obtain the seafood mushroom fermentation seed liquid. The preparation method of the PDA liquid fermentation medium is as follows: weigh 200g of peeled potatoes, add 1000mL of water, boil for 30 minutes, filter and take the filtrate, add 20g of glucose to make up the total volume to 1000mL, and sterilize to obtain the liquid.
7. The method for preparing a Ganoderma lucidum mycelium hair nourishing emulsion according to claim 1, characterized in that, The preparation method of the Ganoderma lucidum solid-state fermentation culture medium in step three is as follows: Dissolve 0.1-0.3 parts of vitamin B12 in 10 times its weight of water, stir and mix evenly to obtain a vitamin B12 solution; thoroughly mix 35-50 parts of seafood mushroom mycelium mixture and 50-100 parts of soybean residue, then add the vitamin B12 solution, stir evenly, adjust the moisture content to 48-55%, sterilize, and obtain the Ganoderma lucidum solid-state fermentation culture medium; the soybean residue is the residue filtered after making tofu or other soy products from soybeans, which is obtained by drying; the vitamin B12 is food grade.
8. The method for preparing a Ganoderma lucidum mycelium hair growth lotion according to claim 1, characterized in that, In step four, the Ganoderma lucidum fermentation seed liquid is inoculated into the Ganoderma lucidum solid fermentation medium at a weight of 5-10%. In step four, the Ganoderma lucidum fermentation seed liquid is inoculated into the Ganoderma lucidum solid fermentation medium, stirred and mixed evenly, and cultured in the dark for 6-7 days at 22-28℃ and 60-70% humidity. The dark conditions are then changed to indoor diffused light and cultured for another 8-15 days. The obtained fermentation culture is then dried, pulverized and passed through a 40-mesh sieve to obtain Ganoderma lucidum fermentation mycelium. The drying process is freeze-drying or low-temperature blow-drying, and the low-temperature blow-drying temperature does not exceed 45℃.
9. The method for preparing a Ganoderma lucidum mycelium hair nourishing emulsion according to claim 1, characterized in that, In step five, the fermented Ganoderma lucidum mycelium powder is added to 15-30 times its weight in a 75%-90% ethanol aqueous solution, mixed thoroughly, and then ultrasonically extracted for 15-25 minutes at 120-180W and 45-65℃. Solid-liquid separation is performed to obtain extract A and the residue after extraction. The residue after extraction is added to 10-20 times its weight in a 25%-35% glycerol aqueous solution, stirred thoroughly, and extracted by stirring at 45-65℃ and 200-300rpm for 45-60 minutes. Solid-liquid separation is performed to obtain extract B. Extracts A and B are combined and concentrated by vacuum distillation at 35-55℃ to 40-50% of the initial weight to obtain the Ganoderma lucidum active ingredient extract.
10. The method for preparing a Ganoderma lucidum mycelium hair growth lotion according to claim 1, characterized in that, The plant essential oil mentioned in step six① is at least one of lavender essential oil, sandalwood essential oil, bergamot essential oil, and rosemary essential oil; in step six②: under stirring conditions of 200-500 rpm, 1-1.5 parts of glycerin and 0.1 parts of plant essential oil are mixed evenly, then 1-2 parts of cetearyl alcohol are added and mixed evenly, then 0.5-0.8 parts of phenoxyethanol are added and mixed evenly, then 1-2 parts of behenyltrimethylammonium methyl sulfate are added, and finally 5-8 parts of Ganoderma lucidum active ingredient extract and 85-88 parts of deionized water are added, homogenized, and packaged to obtain Ganoderma lucidum mycelium hair nourishing lotion.