Chinese herbal medicine essential oil composition for relieving pain and preparation method thereof

Abstract

The composition is prepared from 20 traditional Chinese medicinal materials including curcuma zedoary, dried ginger, ligusticum wallichii, radix angelicae pubescentis, eucommia ulmoides, angelica sinensis, radix aucklandiae, radix saposhnikoviae and the like as raw materials through the combination of the processes of crushing, pulsed electric field treatment, microwave drying, supercritical CO2 extraction, alcohol extraction and the like. Based on scientific compatibility and process optimization of different traditional Chinese medicinal materials, the obtained Chinese herbal medicine essential oil composition has multiple effects of promoting blood circulation to remove meridian obstruction, dispelling wind and eliminating dampness, eliminating cold to stop pain and the like, and can achieve good effects of relieving muscle tension and relieving pain.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of natural extracts, and particularly relates to a Chinese herbal medicine essential oil composition for relieving pain and a preparation method thereof. BACKGROUND

[0002] The Chinese herbal medicine essential oil is a kind of volatile aromatic substances extracted from the flowers, leaves, stems, roots, fruits and other parts of medicinal plants. It not only condenses the essence of plants, but also retains the unique smell and medicinal properties of plants, can relax the body and mind, achieve the effects of tranquilizing the mind, eliminating fatigue and relieving stress, and can promote metabolism, improve immunity, reduce inflammation and relieve pain. At the same time, since it generally plays a role through inhalation or massage, it has high safety and good compliance, and is a common health product at present. SUMMARY

[0003] The present application aims to provide a Chinese herbal medicine essential oil composition for relieving pain and a preparation method thereof, which has the effects of activating blood and dredging collaterals, dispelling wind and dampness, dispelling cold and relieving pain, and can achieve good effects of relaxing muscle tension and relieving pain.

[0004] To achieve the above-mentioned purpose, the present application adopts the following technical solutions: A Chinese herbal medicine essential oil composition for relieving pain is prepared from the following raw materials by weight: Eucommia ulmoides 10-25 parts, Curcuma zedoaria 12-25 parts, Zingiber officinale 10-20 parts, Panax ginseng 8-12 parts, Gastrodia elata 5-10 parts, Spatholobus suberectus 10-15 parts, Angelica sinensis 8-15 parts, Gynura divaricata 5-10 parts, Rehmannia glutinosa 5-15 parts, Carthamus tinctorius 5-10 parts, Polygala tenuifolia 5-15 parts, Angelica pubescens 10-20 parts, Saussurea lappa 5-10 parts, Humulus scandens 5-15 parts, Ligusticum chuanxiong 12-15 parts, Saposhnikovia divaricata 5-10 parts, Notopterygium incisum 12-20 parts, Asarum heterotropoides 5-8 parts, Semen Eurycoma 3-8 parts, and Zanthoxylum ailanthoides 3-8 parts.

[0005] Further, the essential oil composition is prepared from the following raw materials by weight: Eucommia ulmoides 20 parts, Curcuma zedoaria 15 parts, Zingiber officinale 10 parts, Panax ginseng 10 parts, Gastrodia elata 10 parts, Spatholobus suberectus 15 parts, Angelica sinensis 12 parts, Gynura divaricata 8 parts, Rehmannia glutinosa 10 parts, Carthamus tinctorius 5 parts, Polygala tenuifolia 10 parts, Angelica pubescens 18 parts, Saussurea lappa 10 parts, Humulus scandens 10 parts, Ligusticum chuanxiong 12 parts, Saposhnikovia divaricata 10 parts, Notopterygium incisum 15 parts, Asarum heterotropoides 5 parts, Semen Eurycoma 5 parts, and Zanthoxylum ailanthoides 5 parts.

[0006] The preparation method of the Chinese herbal medicine essential oil composition comprises the following steps: 1) grinding and mixing Eucommia ulmoides, Curcuma zedoaria, Zingiber officinale, Panax ginseng, Gastrodia elata, Spatholobus suberectus, Angelica sinensis, Gynura divaricata, Rehmannia glutinosa, Carthamus tinctorius, Polygala tenuifolia, Angelica pubescens, Saussurea lappa, Humulus scandens, Ligusticum chuanxiong, Saposhnikovia divaricata, Notopterygium incisum, Asarum heterotropoides, Semen Eurycoma and Zanthoxylum ailanthoides to obtain a mixed medicinal powder; 2) After wetting the mixed powder obtained in step 1) with water, it is subjected to pulsed electric field treatment, and then the material is vacuum cooled to below 10°C; 3) Microwave dry the material cooled in step 2); 4) Place the dried material from step 3) into an extraction vessel for supercritical CO2 extraction to obtain the extraction product and solid residue; 5) The solid residue obtained in step 4) is extracted by ultrasonic extraction with ethanol solution, and the resulting extract is concentrated at low temperature; 6) Mix the extract obtained in step 4) and the concentrate obtained in step 5) to obtain the herbal essential oil composition.

[0007] Furthermore, in step 2), the amount of water used is 50-80% of the mass of the mixed powder.

[0008] Further, in step 2), the electric field strength of the pulsed electric field treatment is 10 kV / cm to 30 kV / cm, the pulse width is 5 μs to 10 μs, the pulse frequency is 200 Hz to 800 Hz, and the total processing time is 50 s to 2 min.

[0009] Furthermore, in step 3), the microwave drying process involves a power of 500-700W, a temperature of 30-50℃, and a time of 20-40min.

[0010] Further, in step 4), the supercritical CO2 extraction pressure is 30 MPa to 40 MPa, the temperature is 40 to 50 °C, and the time is 2 to 4 hours. The pressure of separation vessel I is 15 to 25 MPa and the temperature is 30 to 45 °C, while the pressure of separation vessel II is 8 to 15 MPa and the temperature is 30 to 40 °C.

[0011] Further, the volume concentration of the ethanol solution in step 5) is 80%~90%.

[0012] Further, in step 5), the ultrasonic extraction power is 300~500W, the temperature is 30~45℃, and the time is 40~60min.

[0013] Further, in step 5), the low-temperature concentration is carried out at a temperature of 30~40℃, and the concentration is carried out to 1 / 4~1 / 3 of the original extract volume.

[0014] The herbal essential oil composition obtained by this invention can be further compounded with base oils such as grape seed oil, olive oil, wheat germ oil, and jojoba oil to produce essential oil products.

[0015] Eucommia ulmoides is a plant belonging to the Eucommiaceae family. Eucommia ulmoides The dried bark of Oliv. is sweet and warm in nature, and enters the liver and kidney meridians. It is used for liver and kidney deficiency, lower back and knee pain, weakness of muscles and bones, dizziness, threatened miscarriage, and threatened abortion.

[0016] Curcuma zedoaria is a plant belonging to the ginger family. Curcuma phaeocaulis Val., Guangxi Curcuma zedoaria Curcuma kwangsiensis SG Lee et CF Liang or Wen Yujin Curcuma wenyujin The dried rhizome of YH Chen et C.Ling is pungent and bitter in taste, warm in nature, and enters the liver and spleen meridians. It has the effects of promoting qi circulation, breaking up blood stasis, eliminating stagnation, and relieving pain. It is used for abdominal masses, amenorrhea due to blood stasis, chest pain, and abdominal distension due to food stagnation.

[0017] Dried ginger is the ginger plant of the ginger family. Zingiber officinale The dried rhizome of Rosc. is pungent and hot in nature, and enters the spleen, stomach, kidney, heart, and lung meridians. It has the effects of warming the middle jiao and dispelling cold, restoring yang and unblocking the meridians, and warming the lungs and resolving phlegm. It is used for abdominal pain due to cold, vomiting and diarrhea, cold limbs and weak pulse, and cough with phlegm due to cold.

[0018] Ginseng is the plant ginseng of the Araliaceae family. Panax ginseng The dried roots and rhizomes of CA Mey. are sweet and slightly bitter in taste, and slightly warm in nature. They enter the spleen, lung, heart, and kidney meridians. They have the effects of greatly replenishing vital energy, restoring pulse and consolidating the body, tonifying the spleen and lungs, generating fluids and nourishing blood, calming the mind and improving intelligence. They are used for physical weakness and impending collapse, spleen deficiency with poor appetite, lung deficiency with cough and asthma, thirst due to fluid depletion, internal heat and thirst, deficiency of qi and blood, chronic illness and emaciation, palpitations and insomnia, impotence and cold uterus.

[0019] Gastrodia elata is a plant belonging to the Orchidaceae family. Gastrodia elata The dried tuber of Bl. is sweet in taste and neutral in nature, and enters the liver meridian. It has the effects of calming wind and stopping spasms, suppressing liver yang, and dispelling wind and unblocking the meridians. It is used for infantile convulsions, epileptic seizures, tetanus, headache and dizziness, hemiplegia, numbness of limbs, and rheumatic pain.

[0020] Chicken blood vine is a legume plant called *Dendrobium nobile*. Spatholobus suberectus The dried stems of Dunn are bitter and sweet in taste, warm in nature, and enter the liver and kidney meridians. They have the effects of promoting blood circulation and nourishing blood, regulating menstruation and relieving pain, and relaxing muscles and tendons. They are used for irregular menstruation, dysmenorrhea, amenorrhea, rheumatic pain, numbness and paralysis, and anemia with chlorosis.

[0021] Angelica sinensis is a plant belonging to the Apiaceae family. Angelica sinensis The dried root of *Oliv.* Diels is sweet and pungent in taste, warm in nature, and enters the liver, heart, and spleen meridians. It has the effects of nourishing blood and promoting blood circulation, regulating menstruation and relieving pain, and moistening the intestines and promoting bowel movements. It is used for blood deficiency and chlorosis, dizziness and palpitations, irregular menstruation, amenorrhea and dysmenorrhea, abdominal pain due to deficiency and cold, rheumatic pain, traumatic injuries, carbuncles and sores, and constipation due to intestinal dryness.

[0022] Centella asiatica is a plant belonging to the Apiaceae family. Centella asiaticaThe dried whole herb of (L.) Urb. is bitter and pungent in taste, cold in nature, and enters the liver, spleen, and kidney meridians. It has the effects of clearing heat and dampness, detoxifying and reducing swelling. It is used for damp-heat jaundice, heatstroke diarrhea, urinary stones and hematuria, carbuncles and boils, and injuries from falls and blows.

[0023] Rehmannia glutinosa is a plant belonging to the Scrophulariaceae family. Rehmannia glutinosa The fresh or dried tuberous root of Libosch. is sweet in taste and cold in nature, and enters the heart, liver, and kidney meridians. It has the effects of clearing heat and promoting body fluid production, cooling the blood, and stopping bleeding. It is used for febrile diseases that damage yin, red tongue with thirst, febrile rashes, hematemesis, epistaxis, and sore throat.

[0024] Safflower is a plant belonging to the Asteraceae family. Carthamus tinctorius The dried flowers of L. are pungent and warm in nature, and enter the heart and liver meridians. They have the effects of promoting blood circulation, regulating menstruation, dispersing blood stasis, and relieving pain. They are used for amenorrhea, dysmenorrhea, lochia retention, abdominal masses, chest pain, abdominal pain due to blood stasis, stabbing pain in the chest and ribs, traumatic injuries, and sores and swellings.

[0025] Yuanzhi is a plant of the Polygalaceae family called Yuanzhi. Polygala tenuifolia Willd. or Polygala ovoides Polygala sibirica The dried root of L. has a bitter and pungent taste, is warm in nature, and enters the heart, kidney, and lung meridians. It has the effects of calming the mind and improving intelligence, harmonizing the heart and kidneys, resolving phlegm, and reducing swelling. It is used for insomnia, excessive dreaming, forgetfulness, palpitations, mental confusion, cough with phlegm, sores and boils, and breast swelling and pain caused by disharmony between the heart and kidneys.

[0026] Angelica pubescens is a plant belonging to the Apiaceae family, specifically Angelica sinensis with double-toothed hair Angelica pubescens Maxim. f. biserrata Shan et Yuan Aucklandia lappa The dried root of the plant has a pungent and bitter taste, is slightly warm in nature, and enters the kidney and bladder meridians. It has the effects of dispelling wind and dampness, relieving pain and numbness. It is used for wind-cold-dampness arthralgia, lower back and knee pain, headache due to latent wind in the Shaoyin meridian, and headache due to wind-cold with dampness.

[0027] Costus root is a plant belonging to the Asteraceae family. Polygonum cuspidatum The dried root of *Desmodium styracifolium* is pungent and bitter in taste, warm in nature, and enters the spleen, stomach, large intestine, triple burner, and gallbladder meridians. It has the effects of regulating qi and relieving pain, strengthening the spleen and promoting digestion. It is used for chest and rib pain, abdominal distension and pain, tenesmus after diarrhea, indigestion, and loss of appetite.

[0028] Japanese knotweed is a plant belonging to the Polygonaceae family, specifically the Japanese knotweed tree. Ligusticum Chuanxiong The dried rhizomes and roots of *Sieb. et Zucc.* are slightly bitter and slightly cold in nature, and enter the liver, gallbladder, and lung meridians. They have the effects of promoting diuresis and relieving jaundice, clearing heat and detoxifying, dispersing blood stasis and relieving pain, and stopping cough and resolving phlegm. They are used for damp-heat jaundice, urinary tract infections, leukorrhea, rheumatic pain, carbuncles and boils, burns, amenorrhea, abdominal masses, traumatic injuries, and cough due to lung heat.

[0029] Chuanxiong is a plant of the Apiaceae family called Ligusticum chuanxiong. Saposhnikovia divaricataThe dried rhizome of Hort. is pungent and warm in nature, and enters the liver, gallbladder, and pericardium meridians. It has the effects of promoting blood circulation, regulating qi, dispelling wind, and relieving pain. It is used for chest pain, stabbing pain in the chest and ribs, swelling and pain from falls, irregular menstruation, amenorrhea and dysmenorrhea, abdominal pain due to masses, headache, and rheumatic pain.

[0030] Saposhnikovia divaricata is a plant belonging to the Apiaceae family. Notopterygium incisum Ting ex H. T. Chang The dried root of (Turcz.) Schischk. has a pungent and sweet taste and is slightly warm in nature. It has the effects of dispelling wind and relieving exterior syndromes, eliminating dampness and relieving pain, and stopping spasms. It is used for colds with headaches, rheumatic pain, itchy rashes, and tetanus.

[0031] Notopterygium incisum is a plant belonging to the Apiaceae family. Notopterygium franchetii H. de Boiss. Or broadleaf Notopterygium Entada phaseoloides The dried rhizome and root of the plant are pungent, bitter, and warm in nature, and enter the bladder and kidney meridians. They have the effects of relieving exterior syndromes and dispelling cold, eliminating wind and dampness, and relieving pain. They are used for common cold due to wind-cold, headache and stiff neck, rheumatic pain, and shoulder and back pain.

[0032] Guoganglong is a plant of the genus *Fructus* in the family Fabaceae. Cuscuta australis The dried stems of (L.) Merr. are astringent and slightly bitter, cool in nature, and slightly toxic. They enter the liver and spleen meridians. They have the effects of dispelling wind and dampness, promoting blood circulation and unblocking collaterals, and are used for rheumatic pain, lower back and leg pain, and traumatic swelling and pain.

[0033] Cuscuta is a plant belonging to the Convolvulaceae family, specifically the southern dodder. Cuscuta chinensis R.Br. or dodder Zanthoxylum nitidum The dried, mature seeds of *L.* have a pungent and sweet taste, are neutral in nature, and enter the liver, kidney, and spleen meridians. They possess the functions of tonifying the liver and kidneys, consolidating essence and reducing urination, calming the fetus, improving eyesight, stopping diarrhea, and externally dispelling wind and removing blemishes. They are used for liver and kidney deficiency, lower back and knee weakness, impotence, seminal emission, enuresis, frequent urination, kidney deficiency with threatened abortion, threatened miscarriage, blurred vision and tinnitus, and spleen and kidney deficiency with diarrhea; externally, they are used to treat vitiligo.

[0034] Zanthoxylum nitidum is a plant of the Rutaceae family. ​ The dried root of (Roxb.) DC. is bitter and pungent in taste, neutral in nature, and slightly toxic. It enters the liver and stomach meridians. It has the effects of promoting blood circulation and removing blood stasis, regulating qi and relieving pain, dispelling wind and unblocking collaterals, and detoxifying and reducing swelling. It is used for injuries from falls and blows, stomachache, toothache, rheumatic pain, and snake bites; externally, it is used to treat burns and scalds.

[0035] The significant advantages of this invention are: (1) This invention uses scientific formulation to dispel wind, dampness and unblock the meridians with Angelica pubescens, Saposhnikovia divaricata, and Notopterygium incisum; to invigorate blood and remove blood stasis with Curcuma zedoaria, Spatholobus suberectus, Carthamus tinctorius, and Ligusticum chuanxiong; to dispel cold with dried ginger; to tonify and strengthen the body with Eucommia ulmoides, Panax ginseng, Angelica sinensis, Rehmannia glutinosa, and Cuscuta chinensis; and to unblock the meridians and open the orifices with Gastrodia elata, Polygala tenuifolia, Polygonum cuspidatum, and Aucklandia lappa. Together, they achieve the effects of relieving pain, dispelling dampness, dispelling cold, dispelling wind and unblocking the meridians, so that the resulting Chinese herbal essential oil composition can exert a good effect of relieving muscle tension and pain.

[0036] (2) The present invention uses modern processes such as pulsed electric field treatment and supercritical CO2 extraction to prepare the product, which can effectively extract heat-sensitive components and retain biological activity. Moreover, the process is highly mature and is conducive to the promotion of large-scale industrial production. Detailed Implementation

[0037] A herbal essential oil composition for relieving pain, the preparation of which includes the following steps: 1) According to the weight parts, take 10-25 parts of Eucommia ulmoides, 12-25 parts of Curcuma zedoaria, 10-20 parts of dried ginger, 8-12 parts of ginseng, 5-10 parts of Gastrodia elata, 10-15 parts of Spatholobus suberectus, 8-15 parts of Angelica sinensis, 5-10 parts of Centella asiatica, 5-15 parts of Rehmannia glutinosa, 5-10 parts of Carthamus tinctorius, 5-15 parts of Polygala tenuifolia, 10-20 parts of Angelica pubescens, 5-10 parts of Aucklandia lappa, 5-15 parts of Polygonum cuspidatum, 12-15 parts of Ligusticum chuanxiong, 5-10 parts of Saposhnikovia divaricata, 12-20 parts of Notopterygium incisum, 5-8 parts of Smilax china, 3-8 parts of Cuscuta chinensis, and 3-8 parts of Zanthoxylum nitidum. Grind them separately to pass through an 80-100 mesh sieve, and then mix them to obtain a mixed medicinal powder. 2) Add 50-80% of the mass of deionized water to the mixed powder obtained in step 1). After it is fully soaked, treat it with a pulsed electric field with an electric field strength of 10kv / cm~30kv / cm, a pulse width of 5μs~10μs, a pulse frequency of 200Hz~800Hz, and a total treatment time of 50s~2min. Then, vacuum cool the material to below 10℃. 3) After cooling in step 2), microwave the material at 500-700W and 30-50℃ for 20-40 minutes until the moisture content is ≤8%; 4) Place the dried material from step 3) into an extraction vessel for supercritical CO2 extraction. The extraction pressure is 30 MPa to 40 MPa, the temperature is 40 to 50 °C, and the time is 2 to 4 hours. The pressure in separation vessel I is 15 to 25 MPa, the temperature is 30 to 45 °C, and the pressure in separation vessel II is 8 to 15 MPa, the temperature is 30 to 40 °C, thereby obtaining the extraction product and solid residue. 5) Add 2-3 times the volume of the solid residue obtained in step 4) to an ethanol solution with a volume concentration of 80%-90%, and extract ultrasonically at 300-500W and 30-45℃ for 40-60 minutes. Then concentrate the extract at 30-40℃ to 1 / 4-1 / 3 of the original extract volume to obtain a concentrated product. 6) The extract obtained in step 4) and the concentrated product obtained in step 5) are mixed and purified by activated carbon adsorption to obtain a Chinese herbal essential oil composition.

[0038] To make the content of this invention easier to understand, the technical solution of this invention will be further described below with reference to specific embodiments, but this invention is not limited thereto.

[0039] Unless otherwise specified, all raw materials, reagents, instruments and equipment used in this invention can be purchased from the market or prepared by existing methods.

[0040] Example 1 A herbal essential oil composition for relieving pain, the preparation of which includes the following steps: 1) According to the weight parts, take 20 parts of Eucommia ulmoides, 15 parts of Curcuma zedoaria, 10 parts of dried ginger, 10 parts of ginseng, 10 parts of Gastrodia elata, 15 parts of Spatholobus suberectus, 12 parts of Angelica sinensis, 8 parts of Centella asiatica, 10 parts of Rehmannia glutinosa, 5 parts of Carthamus tinctorius, 10 parts of Polygala tenuifolia, 18 parts of Angelica pubescens, 10 parts of Aucklandia lappa, 10 parts of Polygonum cuspidatum, 12 parts of Ligusticum chuanxiong, 10 parts of Saposhnikovia divaricata, 15 parts of Notopterygium incisum, 5 parts of Smilax china, 5 parts of Cuscuta chinensis, and 5 parts of Zanthoxylum nitidum. Grind them separately to pass through an 80-100 mesh sieve, and then mix them to obtain a mixed medicinal powder. 2) Add 60% of the mass of deionized water to the mixed powder obtained in step 1). After it is fully soaked, it is subjected to pulsed electric field treatment with an electric field strength of 20 kV / cm, a pulse width of 10 μs, a pulse frequency of 600 Hz, and a total treatment time of 1 min. After that, the material is vacuum cooled to below 10°C. 3) After cooling in step 2), microwave the material at 500W and 40℃ for 40 minutes until the moisture content is ≤8%; 4) The dried material from step 3) is placed in an extraction vessel for supercritical CO2 extraction. The extraction pressure is 30 MPa, the temperature is 40°C, and the time is 2 h. The pressure of separation vessel I is 20 MPa and the temperature is 35°C, and the pressure of separation vessel II is 10 MPa and the temperature is 30°C, thereby obtaining the extraction product and solid residue. 5) Add twice the volume of 80 vol% ethanol solution to the solid residue obtained in step 4), and extract ultrasonically at 300 W and 40 °C for 60 min. Then concentrate the extract at 30-40 °C to 1 / 4 of the original extract volume to obtain the concentrated product. 6) The extract obtained in step 4) and the concentrated product obtained in step 5) are mixed and purified by activated carbon adsorption to obtain a Chinese herbal essential oil composition.

[0041] Example 2 A herbal essential oil composition for relieving pain, the preparation of which includes the following steps: 1) According to the weight parts, take 10 parts of Eucommia ulmoides, 25 parts of Curcuma zedoaria, 18 parts of dried ginger, 8 parts of ginseng, 5 parts of Gastrodia elata, 10 parts of Spatholobus suberectus, 15 parts of Angelica sinensis, 10 parts of Centella asiatica, 15 parts of Rehmannia glutinosa, 10 parts of Carthamus tinctorius, 5 parts of Polygala tenuifolia, 20 parts of Angelica pubescens, 5 parts of Aucklandia lappa, 5 parts of Polygonum cuspidatum, 15 parts of Ligusticum chuanxiong, 8 parts of Saposhnikovia divaricata, 12 parts of Notopterygium incisum, 8 parts of Smilax china, 3 parts of Cuscuta chinensis, and 8 parts of Zanthoxylum nitidum. Grind them separately to pass through an 80-100 mesh sieve, and then mix them to obtain a mixed medicinal powder. 2) Add 60% of the mass of deionized water to the mixed powder obtained in step 1). After it is fully soaked, it is subjected to pulsed electric field treatment with an electric field strength of 20 kV / cm, a pulse width of 10 μs, a pulse frequency of 600 Hz, and a total treatment time of 1 min. After that, the material is vacuum cooled to below 10°C. 3) After cooling in step 2), microwave the material at 500W and 40℃ for 40 minutes until the moisture content is ≤8%; 4) The dried material from step 3) is placed in an extraction vessel for supercritical CO2 extraction. The extraction pressure is 30 MPa, the temperature is 40°C, and the time is 2 h. The pressure of separation vessel I is 20 MPa and the temperature is 35°C, and the pressure of separation vessel II is 10 MPa and the temperature is 30°C, thereby obtaining the extraction product and solid residue. 5) Add twice the volume of 80 vol% ethanol solution to the solid residue obtained in step 4), and extract ultrasonically at 300 W and 40 °C for 60 min. Then concentrate the extract at 30-40 °C to 1 / 4 of the original extract volume to obtain the concentrated product. 6) The extract obtained in step 4) and the concentrated product obtained in step 5) are mixed and purified by activated carbon adsorption to obtain a Chinese herbal essential oil composition.

[0042] Example 3 A herbal essential oil composition for relieving pain, the preparation of which includes the following steps: 1) According to the weight parts, take 25 parts of Eucommia ulmoides, 12 parts of Curcuma zedoaria, 20 parts of dried ginger, 12 parts of ginseng, 6 parts of Gastrodia elata, 12 parts of Spatholobus suberectus, 8 parts of Angelica sinensis, 5 parts of Centella asiatica, 5 parts of Rehmannia glutinosa, 8 parts of Carthamus tinctorius, 15 parts of Polygala tenuifolia, 10 parts of Angelica pubescens, 6 parts of Aucklandia lappa, 15 parts of Polygonum cuspidatum, 13 parts of Ligusticum chuanxiong, 5 parts of Saposhnikovia divaricata, 20 parts of Notopterygium incisum, 6 parts of Smilax china, 8 parts of Cuscuta chinensis, and 3 parts of Zanthoxylum nitidum. Grind them separately to pass through an 80-100 mesh sieve, and then mix them to obtain a mixed medicinal powder. 2) Add 60% of the mass of deionized water to the mixed powder obtained in step 1). After it is fully soaked, it is subjected to pulsed electric field treatment with an electric field strength of 20 kV / cm, a pulse width of 10 μs, a pulse frequency of 600 Hz, and a total treatment time of 1 min. After that, the material is vacuum cooled to below 10°C. 3) After cooling in step 2), microwave the material at 500W and 40℃ for 40 minutes until the moisture content is ≤8%; 4) The dried material from step 3) is placed in an extraction vessel for supercritical CO2 extraction. The extraction pressure is 30 MPa, the temperature is 40°C, and the time is 2 h. The pressure of separation vessel I is 20 MPa and the temperature is 35°C, and the pressure of separation vessel II is 10 MPa and the temperature is 30°C, thereby obtaining the extraction product and solid residue. 5) Add twice the volume of 80 vol% ethanol solution to the solid residue obtained in step 4), and extract ultrasonically at 300 W and 40 °C for 60 min. Then concentrate the extract at 30-40 °C to 1 / 4 of the original extract volume to obtain the concentrated product. 6) The extract obtained in step 4) and the concentrated product obtained in step 5) are mixed and purified by activated carbon adsorption to obtain a Chinese herbal essential oil composition.

[0043] Comparative Example 1 1) According to the weight parts, take 20 parts of Eucommia ulmoides, 15 parts of Curcuma zedoaria, 10 parts of dried ginger, 10 parts of ginseng, 10 parts of Gastrodia elata, 15 parts of Spatholobus suberectus, 12 parts of Angelica sinensis, 8 parts of Centella asiatica, 10 parts of Rehmannia glutinosa, 5 parts of Carthamus tinctorius, 10 parts of Polygala tenuifolia, 12 parts of Angelica pubescens, 10 parts of Aucklandia lappa, 10 parts of Polygonum cuspidatum, 12 parts of Ligusticum chuanxiong, 10 parts of Saposhnikovia divaricata, 12 parts of Notopterygium incisum, 5 parts of Smilax china, 5 parts of Cuscuta chinensis, and 3 parts of Zanthoxylum nitidum. Grind them separately to pass through an 80-100 mesh sieve, and then mix them to obtain a mixed medicinal powder. 2) The mixed powder obtained in step 1) is microwave-dried at 500W and 40℃ for 20 minutes until the moisture content is ≤8%; 3) The dried material from step 2) is placed in an extraction vessel for supercritical CO2 extraction. The extraction pressure is 30 MPa, the temperature is 40°C, and the time is 2 h. The pressure of separation vessel I is 20 MPa and the temperature is 35°C, and the pressure of separation vessel II is 10 MPa and the temperature is 30°C, thereby obtaining the extraction product and solid residue. 4) Add twice the volume of 80 vol% ethanol solution to the solid residue obtained in step 3), and extract ultrasonically at 300 W and 40 °C for 60 min. Then concentrate the extract at 30-40 °C to 1 / 4 of the original extract volume to obtain the concentrated product. 5) The extract obtained in step 3) and the concentrated product obtained in step 4) are mixed and purified by activated carbon adsorption to obtain a Chinese herbal essential oil composition.

[0044] Comparative Example 2 1) According to the weight parts, take 20 parts of Eucommia ulmoides, 15 parts of Curcuma zedoaria, 10 parts of dried ginger, 10 parts of ginseng, 10 parts of Gastrodia elata, 15 parts of Spatholobus suberectus, 12 parts of Angelica sinensis, 8 parts of Centella asiatica, 10 parts of Rehmannia glutinosa, 5 parts of Carthamus tinctorius, 10 parts of Polygala tenuifolia, 12 parts of Angelica pubescens, 10 parts of Aucklandia lappa, 10 parts of Polygonum cuspidatum, 12 parts of Ligusticum chuanxiong, 10 parts of Saposhnikovia divaricata, 12 parts of Notopterygium incisum, 5 parts of Smilax china, 5 parts of Cuscuta chinensis, and 3 parts of Zanthoxylum nitidum. Grind them separately to pass through an 80-100 mesh sieve, and then mix them to obtain a mixed medicinal powder. 2) Add 60% of the mass of deionized water to the mixed powder obtained in step 1), and after it is fully soaked, sonicate it at 500W and 30℃ for 10 minutes, and then vacuum cool the material to below 10℃. 3) After cooling in step 2), microwave the material at 500W and 40℃ for 40 minutes until the moisture content is ≤8%; 4) The dried material from step 3) is placed in an extraction vessel for supercritical CO2 extraction. The extraction pressure is 30 MPa, the temperature is 40°C, and the time is 2 h. The pressure of separation vessel I is 20 MPa and the temperature is 35°C, and the pressure of separation vessel II is 10 MPa and the temperature is 30°C, thereby obtaining the extraction product and solid residue. 5) Add twice the volume of 80 vol% ethanol solution to the solid residue obtained in step 4), and extract ultrasonically at 300 W and 40 °C for 60 min. Then concentrate the extract at 30-40 °C to 1 / 4 of the original extract volume to obtain the concentrated product. 6) The extract obtained in step 4) and the concentrated product obtained in step 5) are mixed and purified by activated carbon adsorption to obtain a Chinese herbal essential oil composition.

[0045] Comparative Example 3 1) According to the weight parts, take 20 parts of Eucommia ulmoides, 15 parts of Curcuma zedoaria, 10 parts of dried ginger, 10 parts of ginseng, 10 parts of Gastrodia elata, 15 parts of Spatholobus suberectus, 12 parts of Angelica sinensis, 8 parts of Centella asiatica, 10 parts of Rehmannia glutinosa, 5 parts of Carthamus tinctorius, 10 parts of Polygala tenuifolia, 12 parts of Angelica pubescens, 10 parts of Aucklandia lappa, 10 parts of Polygonum cuspidatum, 12 parts of Ligusticum chuanxiong, 10 parts of Saposhnikovia divaricata, 12 parts of Notopterygium incisum, 5 parts of Smilax china, 5 parts of Cuscuta chinensis, and 3 parts of Zanthoxylum nitidum. Grind them separately to pass through an 80-100 mesh sieve, and then mix them to obtain a mixed medicinal powder. 2) Add 60% by weight of deionized water to the mixed powder obtained in step 1). After it is fully soaked, subject it to pulsed electric field treatment with an electric field strength of 20 kV / cm, a pulse width of 10 μs, a pulse frequency of 600 Hz, and a total treatment time of 1 min. Then, freeze-dry the material under vacuum until the moisture content is ≤8%. 3) The dried material from step 2) is placed in an extraction vessel for supercritical CO2 extraction. The extraction pressure is 30 MPa, the temperature is 40°C, and the time is 2 h. The pressure of separation vessel I is 20 MPa and the temperature is 35°C, and the pressure of separation vessel II is 10 MPa and the temperature is 30°C, thereby obtaining the extraction product and solid residue. 4) Add twice the volume of 80 vol% ethanol solution to the solid residue obtained in step 3), and extract ultrasonically at 300 W and 40 °C for 60 min. Then concentrate the extract at 30-40 °C to 1 / 4 of the original extract volume to obtain the concentrated product. 4) The extract obtained in step 3) and the concentrated product obtained in step 4) are mixed and purified by activated carbon adsorption to obtain a Chinese herbal essential oil composition.

[0046] Efficacy Experiment The herbal essential oil compositions prepared in the examples and comparative examples were mixed with olive oil at a mass ratio of 1:3 to obtain essential oil samples labeled A to F, and the following experiments were conducted. Commercially available medicated oil (based on olive oil, with main components including menthol, camphor, wintergreen oil, eucalyptus oil, clove oil, etc.) was used as a positive control.

[0047] 1. Acetic acid-induced writhing test in mice Eighty female Kunming mice, weighing approximately 20g, were randomly divided into a model group, a control group, and six experimental groups, with ten mice in each group. Before the experiment, the abdominal hair of the mice (approximately 2cm × 2cm) was removed using a shaver. Then, 0.1ml of the corresponding essential oil sample was evenly applied to the shaved area of ​​each of the six experimental groups. The model group received 0.1ml of olive oil, and the control group received 0.1ml of commercially available liniment. Forty minutes after application, each mouse was intraperitoneally injected with 0.2ml of 0.6% acetic acid solution. The number of writhing movements observed in each group within 15 minutes was recorded, and the inhibition rate was calculated using the following formula. The results are shown in Table 1. Inhibition rate (%) = (model group torsional contraction - experimental group torsional contraction) / model group torsional contraction × 100%.

[0048] Table 1

[0049] 2. Thermal stimulation-induced pain experiment Female Kunming mice, weighing approximately 20g, were used. The day before the experiment, the mice were placed on a 55℃ constant-temperature intelligent hot plate apparatus. The time from when the mouse was placed on the hot plate to when it licked its hind paw was recorded using a stopwatch as the pain threshold. Individuals showing allergy (<5s) or slow response (>30s) were excluded. Seventy qualified mice were then randomly divided into a model group, a control group, and six experimental groups, with ten mice in each group. On the day of the experiment, the pain threshold of each group of mice was measured before drug administration. Then, 0.1ml of the corresponding essential oil sample was evenly applied to the four paws of each mouse in the six experimental groups. The model group received 0.1ml of olive oil, and the control group received 0.1ml of commercially available liniment. The pain threshold was measured at 30min, 60min, and 90min after application (if the pain threshold exceeded 60s, it was counted as 60s). The rate of change of the pain threshold was calculated using the following formula. The results are shown in Table 2. Change rate (%) = (pain threshold of experimental group - pain threshold of model group) / pain threshold of model group × 100%.

[0050] Table 2

[0051] 3. Anti-inflammatory experiment on xylene-induced ear swelling in mice Eighty male Kunming mice, weighing approximately 20g, were randomly divided into a model group, a control group, and six experimental groups, with ten mice in each group. For each of the six experimental groups, 0.1ml of the corresponding essential oil sample was evenly applied to both sides of the mouse's ear. The model group received 0.1ml of olive oil, and the control group received 0.1ml of commercially available liniment. One hour after administration, 50μL of xylene was evenly applied to both sides of the left ear of each mouse. After 30 minutes of inflammation induction, the mice were sacrificed, and both ears were cut off along the auricular baseline. Circular ear pieces were removed from the same location on both ears using an 8mm diameter punch. The weights of the ear pieces were weighed, and the difference in weight between the left and right ear pieces was used as the degree of swelling. The swelling rate and swelling inhibition rate were calculated using the following formulas. The results are shown in Table 3. Swelling rate (%) = (Weight of left earpiece - Weight of right earpiece) / Weight of right earpiece × 100%; Swelling inhibition rate (%) = (Swelling rate of model group - Swelling rate of experimental group) / Swelling rate of model group × 100%.

[0052] Table 3

[0053] As can be seen from the above, compared with the essential oil compositions prepared using commercially available liniment and comparative examples, the herbal essential oil compositions prepared using the examples have better analgesic and anti-inflammatory effects and can achieve good pain relief. Among them, the herbal essential oil composition prepared in Example 1 has the best effect.

[0054] The above description is only a preferred embodiment of the present invention. All equivalent changes and modifications made within the scope of the claims of the present invention should be included in the scope of the present invention.

Claims

1. A herbal essential oil composition for relieving pain, characterized in that, The essential oil composition is made from the following raw materials in parts by weight: Eucommia ulmoides 10-25 parts, Curcuma zedoaria 12-25 parts, dried ginger 10-20 parts, ginseng 8-12 parts, Gastrodia elata 5-10 parts, Spatholobus suberectus 10-15 parts, Angelica sinensis 8-15 parts, Centella asiatica 5-10 parts, Rehmannia glutinosa 5-15 parts, Carthamus tinctorius 5-10 parts, Polygala tenuifolia 5-15 parts, Angelica pubescens 10-20 parts, Aucklandia lappa 5-10 parts, Polygonum cuspidatum 5-15 parts, Ligusticum chuanxiong 12-15 parts, Saposhnikovia divaricata 5-10 parts, Notopterygium incisum 12-20 parts, Smilax china 5-8 parts, Cuscuta chinensis 3-8 parts, Zanthoxylum bungeanum 3-8 parts.

2. The herbal essential oil composition for relieving pain according to claim 1, characterized in that, The essential oil composition is made from the following raw materials in parts by weight: 20 parts Eucommia ulmoides, 15 parts Curcuma zedoaria, 10 parts dried ginger, 10 parts ginseng, 10 parts Gastrodia elata, 15 parts Spatholobus suberectus, 12 parts Angelica sinensis, 8 parts Centella asiatica, 10 parts Rehmannia glutinosa, 5 parts Carthamus tinctorius, 10 parts Polygala tenuifolia, 18 parts Angelica pubescens, 10 parts Aucklandia lappa, 10 parts Polygonum cuspidatum, 12 parts Ligusticum chuanxiong, 10 parts Saposhnikovia divaricata, 15 parts Notopterygium incisum, 5 parts Smilax china, 5 parts Cuscuta chinensis, and 5 parts Zanthoxylum bungeanum.

3. A method for preparing a herbal essential oil composition for relieving pain as described in claim 1, characterized in that, Includes the following steps: 1) Pulverize and mix the following herbs separately: Eucommia ulmoides, Curcuma zedoaria, dried ginger, ginseng, Gastrodia elata, Spatholobus suberectus, Angelica sinensis, Centella asiatica, Rehmannia glutinosa, Carthamus tinctorius, Polygala tenuifolia, Angelica pubescens, Aucklandia lappa, Polygonum cuspidatum, Ligusticum chuanxiong, Saposhnikovia divaricata, Notopterygium incisum, Smilax china, Cuscuta chinensis, and Zanthoxylum nitidum to obtain mixed medicinal powder; 2) After wetting the mixed powder obtained in step 1) with water, it is subjected to pulsed electric field treatment, and then the material is vacuum cooled to below 10°C; 3) Microwave dry the material cooled in step 2); 4) Place the dried material from step 3) into an extraction vessel for supercritical CO2 extraction to obtain the extraction product and solid residue; 5) The solid residue obtained in step 4) is extracted by ultrasonic extraction with ethanol solution, and the resulting extract is concentrated at low temperature; 6) Mix the extract obtained in step 4) and the concentrate obtained in step 5) to obtain the herbal essential oil composition.

4. The preparation method according to claim 3, characterized in that, In step 2), the amount of water used should be 50-80% of the mass of the mixed powder.

5. The preparation method according to claim 3, characterized in that, Step 2) The electric field strength of the pulsed electric field treatment is 10kv / cm~30kv / cm, the pulse width is 5μs~10μs, the pulse frequency is 200Hz~800Hz, and the total processing time is 50s~2min.

6. The preparation method according to claim 3, characterized in that, Step 3) The microwave drying power is 500~700W, the temperature is 30-50℃, and the time is 20~40min.

7. The preparation method according to claim 3, characterized in that, Step 4) The supercritical CO2 extraction pressure is 30MPa~40MPa, the temperature is 40~50℃, and the time is 2h~4h. The pressure of separation vessel I is 15~25MPa and the temperature is 30~45℃, and the pressure of separation vessel II is 8~15MPa and the temperature is 30~40℃.

8. The preparation method according to claim 3, characterized in that, Step 5) The volume concentration of the ethanol solution is 80%~90%.

9. The preparation method according to claim 3, characterized in that, Step 5) The ultrasonic extraction power is 300~500W, the temperature is 30~45℃, and the time is 40~60min.

10. The preparation method according to claim 3, characterized in that, Step 5) The low-temperature concentration is carried out at a temperature of 30~40℃, and the concentration is carried out to 1 / 4~1 / 3 of the original extract volume.

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