Traditional Chinese medicine composition capable of reducing blood fat and eliminating carotid plaque

Abstract

The invention relates to the technical field of traditional Chinese medicines, and provides a traditional Chinese medicine composition capable of reducing blood fat and eliminating carotid plaques. Comprising the following components in parts by weight: 15-20 parts of radix puerariae, 3-6 parts of natto powder, 8-10 parts of red yeast rice, 2-4 parts of allium macrostemon, 2-4 parts of earthworm protein, 5-8 parts of peach kernels, 2-6 parts of perilla seeds, 1-2 parts of soybean peptide powder, 12-15 parts of a grape seed extract, 8-10 parts of a celery seed extract, 1-2 parts of selenium-enriched triticale peptide, 2-3 parts of saffron and 2-4 parts of turmeric. According to the technical scheme, the problem that a traditional Chinese medicine composition in the related technology is poor in blood fat reducing effect is solved.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of traditional Chinese medicine, in particular, to a traditional Chinese medicine composition for lowering blood lipid and eliminating carotid plaque. BACKGROUND

[0002] Due to the improvement of life quality, hyperlipidemia gradually shows a trend of youth, and has seriously affected the quality of people's life. Most of the hyperlipidemia patients also have hypertension symptoms, which easily leads to the occurrence of cardiovascular diseases such as carotid plaque. If it cannot be prevented and treated early, the consequences are very serious.

[0003] At present, the commonly used drug for lowering blood lipid in clinic is statin, which is a cholesterol biosynthesis enzyme inhibitor. It is the most widely used lipid-regulating drug in clinical application, and is the main drug for treating hypercholesterolemia at present. However, this kind of chemical synthetic drug usually has side effects, which can cause abdominal distension, diarrhea and other gastrointestinal discomfort, headache, and long-term use of chemical drugs may also cause damage to liver, kidney and other organs. Therefore, a safe, effective and less side effect drug for lowering blood lipid is urgently needed. Traditional Chinese medicine has a long history and unique advantages in the field of prevention and treatment of dyslipidemia. However, the existing traditional Chinese medicine composition for lowering blood lipid has insufficient effect, therefore, a more efficient traditional Chinese medicine composition for lowering blood lipid is urgently needed. SUMMARY

[0004] The present application provides a traditional Chinese medicine composition for lowering blood lipid and eliminating carotid plaque, which solves the problem of poor blood lipid-lowering effect of traditional Chinese medicine composition in the related art.

[0005] The technical scheme of the present application is as follows: The present application provides a traditional Chinese medicine composition for lowering blood lipid and eliminating carotid plaque, which includes the following components by weight: 15-20 parts of radix puerariae, 3-6 parts of natto powder, 8-10 parts of red yeast rice, 2-4 parts of allium macrostemon bunge, 2-4 parts of earthworm protein, 5-8 parts of persicae semen, 2-6 parts of perilla frutescens, 1-2 parts of soybean peptide powder, 12-15 parts of grape seed extract, 8-10 parts of celery seed extract, 1-2 parts of selenium-enriched wheat peptide, 2-3 parts of safflower, and 2-4 parts of curcuma longa. The preparation method of the grape seed extract includes the following steps: S1, drying and crushing grape seeds, adding a eutectic solvent, ultrasonic treatment, and centrifugation to obtain a crude grape seed extract; S2, drying the crude grape seed extract after resin purification to obtain the grape seed extract; The eutectic solvent includes a hydrogen bond acceptor and a hydrogen bond donor; The hydrogen bond acceptor is betaine, and the hydrogen bond donor is 1,2-propanediol and a sugar alcohol compound; The sugar alcohol compound includes xylitol.

[0006] Grape seed contains proanthocyanidins, and the structure of proanthocyanidins in grape seed is condensed by flavan-3-ol structural units, and the monomers mainly include epicatechin gallate, catechin, catechin gallate and epicatechin, etc. Proanthocyanidins have very important functions, but in the current proanthocyanidin extraction process, there are problems of low extraction rate and low purity of proanthocyanidins, which will affect the play of the hypolipidemic effect in the application process.

[0007] In the application, betaine, 1,2-propanediol and sugar alcohol compounds are used as the eutectic solvent, so that the extraction rate and purity of grape seed extract can be improved.

[0008] As a further technical solution, the mass ratio of the hydrogen bond acceptor and the hydrogen bond donor is 1:2-4.

[0009] As a further technical solution, the mass ratio of the 1,2-propanediol and the sugar alcohol compound is 1:1-3.

[0010] As a further technical solution, the sugar alcohol compound includes maltitol.

[0011] As a further technical solution, the resin purification includes primary purification and secondary purification in sequence. The primary purification is neutral macroporous adsorption resin purification, and the secondary purification is anion macroporous adsorption resin purification.

[0012] As a further technical solution, the type of the neutral macroporous adsorption resin is LSA-8, and the type of the anion macroporous adsorption resin is D301G.

[0013] In the application, the use of LSA-8 type macroporous adsorption resin and D301G type macroporous adsorption resin for purifying the crude grape seed extract can further improve the extraction rate and purity of the grape seed extract.

[0014] As a further technical solution, the mass-volume ratio of the grape seed and the eutectic solvent is 1g:30-40mL.

[0015] As a further technical solution, the preparation method of the eutectic solvent includes the following steps: mixing betaine, 1,2-propanediol and sugar alcohol compounds, then adding water and stirring to obtain the eutectic solvent.

[0016] As a further technical solution, the mass ratio of the betaine and water is 1:2-3. Preferably, the mass ratio of the betaine and water is 1:2.

[0017] As a further technical solution, the stirring temperature is 60-70℃. Preferably, the temperature of the stirring is 70 DEG C.

[0018] As a further technical solution, the ultrasonic time is 60-80 min; the temperature is 50-60 DEG C.

[0019] As a further technical solution, the centrifugal speed is 7000-8000 r / min, and the time is 30-40 min.

[0020] The application further provides a traditional Chinese medicine decoction piece and a preparation method thereof.

[0021] The working principle and beneficial effects of the application are as follows: In the application, the components of the traditional Chinese medicine composition are optimized, the blood lipid-lowering effect of the traditional Chinese medicine composition is improved, and the composition has the effect of eliminating carotid plaque. Pueraria, red yeast rice, natto powder, Chinese onion, grape seed extract and celery seed extract have the effect of lowering blood lipid; turmeric, peach kernel and saffron have the effects of activating blood and resolving stasis and resisting thrombus; earthworm protein can soften blood vessels and improve serum lipid; perilla seed can reduce the content of triglyceride and cholesterol in blood and regulate blood lipid; it also has the effect of resisting inflammation and can reduce vascular inflammatory response; soybean peptide powder can reduce the absorption of cholesterol; selenium in selenium-rich black wheat peptide has the effect of resisting oxidation; and black wheat peptide can regulate various components of the body metabolism, so that the medicinal properties are coordinated, the effects of lowering blood lipid and eliminating carotid plaque are achieved. DETAILED DESCRIPTION

[0022] The technical solutions in the embodiments of the application will be clearly and completely described in combination with the embodiments of the application. Obviously, the described embodiments are only part of the embodiments of the application, rather than all the embodiments of the application. Based on the embodiments in the application, all other embodiments obtained by those skilled in the art without creative labor also fall within the protection scope of the application.

[0023] In the following examples and comparative examples, the purity of celery seed extract is 80%, and the manufacturer is Chengdu Pusai Biotechnology Co., Ltd.

[0024] Example 1 A traditional Chinese medicine composition for lowering blood lipid and eliminating carotid plaque comprises the following components in parts by weight: pueraria 15 parts, natto powder 3 parts, red yeast rice 8 parts, Chinese onion 2 parts, earthworm protein 2 parts, peach kernel 5 parts, perilla seed 2 parts, soybean peptide powder 1 part, grape seed extract 12 parts, celery seed extract 8 parts, selenium-rich black wheat peptide 1 part, saffron 2 parts and turmeric 2 parts. A method for preparing a deep eutectic solvent, comprising the following steps: mixing 10g betaine, 10g 1,2-propanediol and 10g xylitol, then adding 20g water, stirring in a water bath at 70℃, and obtaining a deep eutectic solvent; A method for preparing grape seed extract, comprising the following steps: S1, 10g of dried and crushed grape seeds are added to 300mL of deep eutectic solvent, then ultrasonic treatment is performed at 50℃ for 80min, centrifugation is performed at 7000r / min for 40min, and a crude grape seed extract is obtained; S2, the crude grape seed extract is purified by LSA-8 type macroporous adsorption resin, and then dried to obtain grape seed extract.

[0025] Example 2 A traditional Chinese medicine composition for lowering blood lipids and eliminating carotid plaque, comprising the following components in parts by weight: 20 parts of radix puerariae, 6 parts of natto powder, 10 parts of red yeast rice, 4 parts of allium macrostemon, 4 parts of earthworm protein, 8 parts of persicae semen, 6 parts of perilla frutescens, 2 parts of soybean peptide powder, 15 parts of grape seed extract, 10 parts of apium graveolus seed extract, 2 parts of selenium-enriched wheat peptide, 3 parts of saffron, and 4 parts of curcuma longa; A method for preparing a deep eutectic solvent, comprising the following steps: mixing 10g betaine, 10g 1,2-propanediol and 30g xylitol, then adding 20g water, stirring in a water bath at 70℃, and obtaining a deep eutectic solvent; A method for preparing grape seed extract, comprising the following steps: S1, 10g of dried and crushed grape seeds are added to 400mL of deep eutectic solvent, then ultrasonic treatment is performed at 60℃ for 60min, centrifugation is performed at 8000r / min for 30min, and a crude grape seed extract is obtained; S2, the crude grape seed extract is purified by LSA-8 type macroporous adsorption resin, and then dried to obtain grape seed extract.

[0026] Example 3 A traditional Chinese medicine composition for lowering blood lipids and eliminating carotid plaque, comprising the following components in parts by weight: 18 parts of radix puerariae, 5 parts of natto powder, 9 parts of red yeast rice, 3 parts of allium macrostemon, 3 parts of earthworm protein, 6 parts of persicae semen, 4 parts of perilla frutescens, 1.5 parts of soybean peptide powder, 13 parts of grape seed extract, 9 parts of apium graveolus seed extract, 1.5 parts of selenium-enriched wheat peptide, 2.5 parts of saffron, and 3 parts of curcuma longa; A method for preparing a deep eutectic solvent, comprising the following steps: mixing 10g betaine, 10g 1,2-propanediol and 20g xylitol, then adding 20g water, stirring in a water bath at 70℃, and obtaining a deep eutectic solvent; A method for preparing grape seed extract, comprising the following steps: S1. Dry and pulverize 10g of grape seeds, add 350mL of eutectic solvent, sonicate at 55℃ for 70min, and centrifuge at 7500r / min for 35min to obtain crude grape seed extract. S2. Crude grape seed extract was purified by LSA-8 macroporous adsorption resin and then dried to obtain grape seed extract.

[0027] Example 4 The only difference between this embodiment and Example 3 is that xylitol is replaced with maltitol when preparing the eutectic solvent.

[0028] Example 5 The only difference between this embodiment and Embodiment 3 is that xylitol is replaced with sorbitol when preparing the eutectic solvent.

[0029] Example 6 The only difference between this embodiment and Example 3 is that xylitol is replaced with lactitol when preparing the eutectic solvent.

[0030] Example 7 The only difference between this embodiment and embodiment 4 is that in step S2, the crude grape seed extract is purified by D301G macroporous adsorption resin and then dried to obtain the grape seed extract.

[0031] Example 8 The only difference between this embodiment and embodiment 4 is that in step S2, the crude grape seed extract is first purified by LSA-8 macroporous adsorption resin, then purified by D301G macroporous adsorption resin, and dried to obtain the grape seed extract.

[0032] Example 9 The only difference between this embodiment and embodiment 4 is that in step S2, the crude grape seed extract is first purified by D301 macroporous adsorption resin, then purified by D301G macroporous adsorption resin, and dried to obtain the grape seed extract.

[0033] Example 10 The only difference between this embodiment and embodiment 4 is that in step S2, the crude grape seed extract is first purified by LSA-8 macroporous adsorption resin, then purified by NKA-9 macroporous adsorption resin, and dried to obtain the grape seed extract.

[0034] Comparative Example 1 The only difference between this comparative example and Example 3 is that the preparation of the eutectic solvent includes the following steps: 10g of betaine and 30g of 1,2-propanediol are mixed and then 20g of water is added. After stirring in a water bath at 70°C, the eutectic solvent is obtained.

[0035] Comparative Example 2 The only difference between this comparative example and Example 3 is that the preparation of the eutectic solvent includes the following steps: 10g betaine and 30g xylitol are mixed and then 20g of water is added. After stirring in a water bath at 70°C, the eutectic solvent is obtained.

[0036] The proanthocyanidin content in grape seed extracts from Examples 1-10 and Comparative Examples 1-2 was measured, and the extraction rate and purity were calculated. Extraction rate = mass of proanthocyanidins in grape seed extract / mass of grape seeds × 100%. Purity is expressed as polyphenol content. The results are shown in Table 1 below.

[0037] Table 1. Results of Grape Seed Extract Determination

[0038] By comparing the data from Example 3 and Comparative Examples 1-2, it was found that Example 3, by using 1,2-propanediol and xylitol as hydrogen bond donors and betaine as a eutectic solvent, significantly improved the extraction rate and purity of the grape seed extract compared to Comparative Examples 1-2. This indicates that using betaine, 1,2-propanediol, and xylitol as eutectic solvents can significantly improve the extraction rate and purity of grape seed extract.

[0039] By comparing the data from Examples 3 and 4-6, it was found that Example 4, by using 1,2-propanediol and maltitol as hydrogen bond donors and betaine as a eutectic solvent, further improved the extraction rate and purity of the grape seed extract compared to Examples 3 and 5-6. This indicates that using betaine, 1,2-propanediol, and maltitol as eutectic solvents can further improve the extraction rate and purity of the grape seed extract.

[0040] By comparing the data from Examples 4 and 7-10, the purity of the grape seed extract obtained in Example 8 by using LSA-8 macroporous adsorption resin and D301G macroporous adsorption resin in combination was higher than that in Examples 4, 7, and 9-10. This indicates that the extraction rate and purity of grape seed extract can be further improved by using LSA-8 macroporous adsorption resin and D301G macroporous adsorption resin in combination.

[0041] Experiment Example 1: Human trial of blood glucose-lowering drugs 1.1 Preparation method of therapeutic drugs By weight, 78.5 parts of the traditional Chinese medicine composition obtained in Example 8 were pulverized and mixed, and then 15 parts of sorbitol, 20 parts of starch and 4 parts of magnesium stearate were added and mixed evenly. Ethanol with a volume fraction of 85% was added to granulate, dry and compress into tablets to obtain traditional Chinese medicine slices (0.6g per tablet).

[0042] 1.2 Trial Results Subjects of the trial: 10 patients with dyslipidemia, numbered sequentially as Subject-1, Subject-2, ... Subject-10; Trial administration method: Each subject should take 5 tablets twice a day, morning and evening; blood lipid levels of each subject before and after 30 days of trial administration should be recorded. The normal range for blood lipid levels is 3.6~6.5, the normal range for TG is 0~1.71, the normal range for HDL is 0.9~2.0, and the normal range for LDL is 2.06~3.1. The specific trial results are shown in Table 2 below.

[0043] Table 2 Trial Results

[0044] According to Table 2, most patients with dyslipidemia showed a decreasing trend in blood lipid indicators TC, TG, and LDL after taking the traditional Chinese medicine decoction pieces prepared in Example 8 of this application for 30 days. Therefore, the traditional Chinese medicine composition in this invention has a certain blood lipid-lowering effect.

[0045] Experiment Example 2: Human trial to eliminate carotid artery plaque 2.1 Trial Results 1. Mr. Zhao, before taking the medicine, had a 4.2×1.4mm plaque on his right carotid artery and a 6.5×2.3mm plaque on his left carotid artery on July 16, 2024, with a media thickness of 1.0mm; after taking the Chinese herbal medicine, the plaques on both carotid arteries disappeared completely on November 20, 2024, and the media thickness returned to normal. 2. Mr. Zhang, 64 years old, before taking the medicine, on June 12, 2021, had bilateral carotid artery atherosclerosis with plaque formation. The plaque in the left carotid artery was 12.4×3.0mm, and the plaque in the right carotid artery was 5.1×2.0mm. After taking the traditional Chinese medicine decoction, on August 29, 2021, the blood flow in both vertebral arteries was normal, the blood flow in the right carotid artery was normal, and the plaque disappeared. 3. Mr. Wang, 63 years old, before taking the medicine, on April 25, had thickened carotid arteries on both sides, with plaque formation in the left carotid artery and decreased elasticity in both carotid arteries; after taking the Chinese herbal medicine, on May 29, the condition of both carotid arteries was good. 4. Ms. Lu, 60 years old, before taking the medicine, on April 18, 2021, had a 0.3×0.16cm plaque in her right carotid artery; after taking the Chinese herbal medicine, on September 11, 2021, there were no abnormalities in both carotid arteries, and the plaque in her right carotid artery disappeared. According to the trial results, the traditional Chinese medicine composition in this invention has a certain effect in eliminating carotid artery plaques.

[0046] The above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A traditional Chinese medicine composition that lowers blood lipids and eliminates carotid artery plaques, characterized in that, The product comprises the following components in parts by weight: 15-20 parts kudzu root, 3-6 parts natto powder, 8-10 parts red yeast rice, 2-4 parts allium macrostemon, 2-4 parts earthworm protein, 5-8 parts peach kernel, 2-6 parts perilla seed, 1-2 parts soybean peptide powder, 12-15 parts grape seed extract, 8-10 parts celery seed extract, 1-2 parts selenium-enriched black wheat peptide, 2-3 parts saffron, and 2-4 parts turmeric; the preparation method of the grape seed extract includes the following steps: S1. Dry and pulverize grape seeds, add eutectic solvent, sonicate, and centrifuge to obtain crude grape seed extract. S2. The crude grape seed extract is purified by resin and then dried to obtain grape seed extract. The eutectic solvent includes hydrogen bond acceptors and hydrogen bond donors; The hydrogen bond acceptor is betaine; the hydrogen bond donor is 1,2-propanediol and sugar alcohols. The sugar alcohols include xylitol.

2. The traditional Chinese medicine composition according to claim 1, which has the functions of lowering blood lipids and eliminating carotid artery plaques, is characterized in that, The mass ratio of the hydrogen bond acceptor to the hydrogen bond donor is 1:2~4.

3. The traditional Chinese medicine composition according to claim 1, which has the functions of lowering blood lipids and eliminating carotid artery plaques, is characterized in that, The mass ratio of the 1,2-propanediol to the sugar alcohol compound is 1:1~3.

4. The traditional Chinese medicine composition according to claim 1, which has the functions of lowering blood lipids and eliminating carotid artery plaques, is characterized in that, The sugar alcohols include maltitol.

5. The traditional Chinese medicine composition according to claim 1, which has the functions of lowering blood lipids and eliminating carotid artery plaques, is characterized in that, The resin purification process includes primary purification and secondary purification. The primary purification is performed using a neutral macroporous adsorption resin; the secondary purification is performed using an anion macroporous adsorption resin.

6. The traditional Chinese medicine composition according to claim 5, which has the functions of lowering blood lipids and eliminating carotid artery plaques, is characterized in that, The neutral macroporous adsorption resin is model LSA-8; the anion macroporous adsorption resin is model D301G.

7. The traditional Chinese medicine composition according to claim 1, which has the functions of lowering blood lipids and eliminating carotid artery plaques, is characterized in that, The mass-to-volume ratio of grape seeds to eutectic solvent is 1g:30~40mL.

8. The traditional Chinese medicine composition according to claim 1, which has the functions of lowering blood lipids and eliminating carotid artery plaques, is characterized in that, The method for preparing the eutectic solvent includes the following steps: mixing betaine, 1,2-propanediol and sugar alcohol compounds, adding water and stirring to obtain the eutectic solvent.

9. A traditional Chinese medicine composition according to claim 1, characterized in that, The ultrasound duration is 60-80 minutes; the temperature is 50-60°C.

10. A traditional Chinese medicine composition according to claim 1, characterized in that, The centrifugation speed is 7000~8000 r / min, and the time is 30~40 min.

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