Extraction equipment and extraction method of marine microalgae xanthophyll

Abstract

The invention provides marine microalgae xanthophyll extraction equipment which comprises a barrel body, a fixing seat is fixed to the side of the barrel body, a fixing column is welded to the top of the fixing seat, a fixing block is movably installed at the top of the fixing column, a cover plate is fixed to one side of the fixing block, a fixing ring box and a driving motor are fixed to the cover plate, and the fixing ring box and the driving motor are fixed to the side of the barrel body. A driving motor is installed on the cover plate, a stirring assembly is installed on the driving motor, the stirring assembly is movably installed in the barrel body, a fixing pipe is fixed to the inner wall of the cover plate, and a spraying base is fixed to the fixing pipe. During use, the rotating column drives the stirring column and the transmission seat to rotate, when the transmission seat rotates, the gears are matched to drive the stirring frame to rotate, the stirring frame rotates to realize up-down stirring, and the stirring column rotates to complete left-right stirring, so that rapid stirring in different directions is completed; in the later period, the stirring frame rotates to push the movable seat to reciprocate up and down, so that the filtering plate is shaken and filtered, and the filtering efficiency is improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of lutein extraction equipment, in particular to a marine microalgae lutein extraction equipment and method. BACKGROUND

[0002] Marine microalgae are rich in lutein, so lutein can be extracted from microalgae. During extraction, a stirring device is needed to break the cell wall for extraction. However, the existing extraction equipment cannot achieve rapid cell wall breaking, and after the cell wall breaking is completed, it cannot achieve shaking filtration, and cannot achieve separation of impurities and liquid.

[0003] According to the patent document with the present disclosure number CN222900272U, a lutein extraction device is disclosed, which includes a stirring barrel, an input pipe is connected through the upper surface of the stirring barrel, an input port is arranged at the end of the input pipe away from the stirring barrel, an input port is arranged on the surface of the input pipe, a stirring assembly is fixedly connected to the upper surface of the stirring barrel, a liquid discharge pipe one is connected through the lower surface of the stirring barrel, a water stop valve is fixedly connected to the other end of the liquid discharge pipe one, a liquid discharge pipe two is fixedly connected inside the left side of the water stop valve, and a filter assembly is connected through the other end of the liquid discharge pipe two. When in use, the spring pushes the upper pressing plate upward, thereby driving the stirring rod upward, and the stirring blade moves upward, so that the stirring blade moves up and down while stirring, greatly shortening the mixing time of plant raw materials and organic solvents, and the lutein can be quickly dissolved into the organic solvent. For the above technical solution, the scheme realizes up and down stirring, but the stirring direction is single, and cannot provide bidirectional stirring while extruding and shaking the filter assembly to accelerate filtration, which is inconvenient in use. SUMMARY

[0004] In view of the deficiencies of the prior art, the present application aims to provide a marine microalgae lutein extraction equipment and method to solve the problems raised in the background art. The present application has a novel structure. In use, the rotating column drives the stirring column and the transmission seat to rotate. The transmission seat rotates and the gears cooperate to drive the stirring frame to rotate. The stirring frame rotates to achieve up and down stirring. The stirring column rotates to complete left and right stirring, thereby completing rapid stirring in different directions. In the later stage, the rotation of the stirring frame drives the movable seat to move up and down reciprocally to achieve shaking filtration of the filter plate, thereby accelerating the filtration efficiency.

[0005] In order to achieve the above object, the present application is realized by the following technical scheme: A marine microalgae lutein extraction equipment, comprising a barrel, a fixed seat is fixed on the side of the barrel, a fixed column is welded on the top of the fixed seat, a fixed block is movably installed on the top of the fixed column, a cover plate is fixed on one side of the fixed block, a fixed ring box and a driving motor are respectively fixed on the cover plate, a stirring assembly is installed on the driving motor, the stirring assembly is movably installed in the barrel, a fixed tube is fixed on the inner wall of the cover plate, a spray seat is fixed on the fixed tube, a connecting pipe is fixed on the spray seat, a ring pipe is fixed between the connecting pipes, and a feed pipe is fixed on the ring pipe.

[0006] Further, the bottom of the spray seat is provided with an opening, an ultrasonic generator is fixedly installed in the inside of the fixed tube, a connecting seat is fixed on the side of the barrel, and a support frame is fixedly installed on the connecting seat.

[0007] Further, the stirring assembly comprises a rotating column, one end of the rotating column is fixed on the driving motor, a stirring column is welded on the rotating column, and a transmission seat is fixed on one end of the rotating column.

[0008] Further, a stirring frame is movably installed in the inside of the transmission seat, a support column is movably installed at the bottom of the transmission seat, a driving bevel gear is fixed on one end of the support column, a driven gear is rotatably installed on the side of the driving bevel gear, and the driven gear is fixed on one end of the stirring frame.

[0009] Further, a lifting mechanism is fixed at the bottom of the barrel, a telescopic column is installed on the lifting mechanism, a positioning column is movably installed in the inside of the telescopic column, and a movable seat is fixed on one end of the positioning column.

[0010] Further, a buffer seat is fixed on the movable seat, a filter plate and a cross rod are fixed between the buffer seat and the movable seat, a support mechanism and a limiting column are fixed on one end of the positioning column, and one end of the support mechanism is fixed in the inside of the telescopic column.

[0011] Further, a discharge port is formed at the bottom of the barrel, a discharge pipe is fixed on one end of the discharge port, a reinforcing rod is welded on the inner wall of the discharge port, and one end of the reinforcing rod is fixed on one end of the support column.

[0012] A marine microalgae lutein extraction method using the equipment of claim 1, the method comprising the following steps:

[0013] Step one: raw material preparation;

[0014] Step two: wall breaking and crushing, composite enzyme is used for enzyme wall breaking, low-osmotic buffer solution is used to swell the cell contents, and ultrasonic wave is used for auxiliary crushing to improve the wall breaking effect;

[0015] Step three: optimization of extraction, using ethanol as a carrier, using uniform design to optimize the extraction conditions, using HPLC and HPLC-MS to detect and identify the content of lutein in the extract, and calculate the extraction rate;

[0016] Step four: water washing collection;

[0017] Step five: crystallization extraction, collecting lutein components, and finally using recrystallization technology for refining to obtain lutein pure product, detecting the content and calculating the extraction rate.

[0018] Further, in the step four, the lutein is separated and purified by column chromatography, the total pigment in the broken Chlorella cells is extracted by acetone, then the lutein is separated from chlorophyll and other carotenoids by reverse phase chromatography using polystyrene-divinylbenzene type reversed phase resin, the solvent ratio of n-hexane-methanol-water elution system is determined, and gradient elution is carried out.

[0019] Further, in the step one, the chlorophyll fluorescence imaging system is used to quickly and high-throughput screen microalgae with strong chlorophyll fluorescence, the preliminary screened microalgae are cultured in large scale, the content of lutein in the algal cells is accurately analyzed by high performance liquid chromatography, and finally the high-quality microalgae with high lutein content are screened.

[0020] The beneficial effects of the present application are:

[0021] 1. In use, the stirring column and the transmission seat are rotated by rotating the column, the stirring frame is rotated by the cooperation of the gears when the transmission seat rotates, the up-down stirring is realized by the rotation of the stirring frame, and the left-right stirring is realized by the rotation of the stirring column, so that the rapid stirring in different directions is realized, and the shaking and filtering of the filter plate are realized by the reciprocating movement of the movable seat driven by the rotation of the stirring frame in the later stage, so that the filtering efficiency is improved.

[0022] 2. In the present application, the liquid material is sprayed to the surface of the fixed tube through the opening when feeding, the liquid material slides into the fixed tube, the ultrasonic generator in the fixed tube contacts the liquid to break the cell wall during the downward falling of the liquid, the stirring is accelerated after the cell wall is broken to improve the extraction efficiency, and the additives are added through the spraying seat in the later stage, so that the additives flow to different positions through the opening to facilitate the rapid mixing in the later stage.

[0023] 3. In the present application, the movable seat is moved up and down by the lifting mechanism, the contact and cooperation of the buffer seat and the stirring frame are controlled by the up-down movement of the movable seat, the movable seat reciprocates by the supporting mechanism under the pushing of the stirring frame, the filter plate reciprocates and impacts the liquid, the impurities on the surface of the filter plate are moved upward under the extrusion of the liquid, so that the blockage is reduced by the shaking of the filter plate. BRIEF DESCRIPTION OF DRAWINGS

[0024] Figure 1 This is a schematic diagram of a method for extracting lutein from marine microalgae according to the present invention;

[0025] Figure 2 This is a schematic diagram of the structure of a marine microalgae lutein extraction device according to the present invention;

[0026] Figure 3 This is a schematic diagram of the structure of the extraction device for lutein from marine microalgae according to the present invention after the cover is opened;

[0027] Figure 4 This is a schematic diagram of the stirring assembly of a marine microalgae lutein extraction device according to the present invention;

[0028] Figure 5 This is a schematic diagram of the support mechanism of a marine microalgae lutein extraction device according to the present invention;

[0029] Figure 6 This is a schematic diagram of the spray base of a marine microalgae lutein extraction device according to the present invention;

[0030] Figure 7 This is a schematic diagram of the opening structure of a marine microalgae lutein extraction device according to the present invention;

[0031] Figure 8 This is a side view cross-sectional structural diagram of the transmission seat of the extraction device for lutein from marine microalgae according to the present invention;

[0032] Figure 9 This is a partial structural diagram of the barrel of a marine microalgae lutein extraction device according to the present invention;

[0033] In the diagram: 1. Barrel body; 2. Fixed seat; 3. Fixed column; 4. Fixed block; 5. Cover plate; 6. Fixed ring box; 7. Drive motor; 8. Feed pipe; 9. Connecting seat; 10. Discharge pipe; 11. Support frame; 12. Fixed pipe; 13. Mixing assembly; 14. Rotating column; 15. Mixing column; 16. Transmission seat; 17. Mixing frame; 18. Support column; 19. Movable seat; 20. Buffer seat; 21. Filter plate; 22. Positioning column; 23. Lifting mechanism; 24. Telescopic column; 25. Limiting column; 26. Support mechanism; 27. Ring pipe; 28. Connecting pipe; 29. ​​Spray seat; 30. Opening; 31. Ultrasonic generator; 32. Driven gear; 33. Driving bevel gear; 34. Reinforcing rod; 35. Discharge port. Detailed Implementation

[0034] To make the technical means, creative features, objectives and effects of this invention easier to understand, the invention will be further described below in conjunction with specific embodiments.

[0035] Please see Figures 1 to 9This invention provides a technical solution: an extraction device for lutein from marine microalgae, comprising a barrel 1, a fixing seat 2 fixed to the side of the barrel 1, a fixing column 3 welded to the top of the fixing seat 2, a fixing block 4 movably mounted on the top of the fixing column 3, a cover plate 5 fixed to one side of the fixing block 4, a fixing ring box 6 and a drive motor 7 respectively fixed on the cover plate 5, a stirring assembly 13 mounted on the drive motor 7, the stirring assembly 13 movably mounted inside the barrel 1, a fixing pipe 12 fixed to the inner wall of the cover plate 5, a spray seat 29 fixed to the fixing pipe 12, and a fixing ring box 6 and a drive motor 7 fixed to the spray seat 29. There is a connecting pipe 28, and a ring pipe 27 is fixed between the connecting pipes 28. A feed pipe 8 is fixed on the ring pipe 27. An opening 30 is opened at the bottom of the spray seat 29. An ultrasonic generator 31 is fixedly installed inside the fixed pipe 12. A connecting seat 9 is fixed on the side of the barrel 1. A support frame 11 is fixedly installed on the connecting seat 9. During the process of the liquid falling downward, the ultrasonic generator 31 inside the fixed pipe 12 comes into contact with the liquid to break the cell wall. After breaking the cell wall, stirring accelerates the extraction efficiency. In addition, additives are added later through the spray seat 29. The additives flow through the opening 30 to different positions to facilitate rapid mixing later.

[0036] In this embodiment, the stirring assembly 13 includes a rotating column 14, one end of which is fixed to a drive motor 7. A stirring column 15 is welded onto the rotating column 14. A transmission seat 16 is fixed to one end of the rotating column 14. A stirring frame 17 is movably installed inside the transmission seat 16. A support column 18 is movably installed at the bottom of the transmission seat 16. A driving bevel gear 33 is fixed to one end of the support column 18. A driven gear 32 is rotatably installed on the side of the driving bevel gear 33. The driven gear 32 is fixed to one end of the stirring frame 17. A lifting mechanism 23 is fixed to the bottom of the barrel 1. A telescopic column 24 is installed on the lifting mechanism 23. A positioning column 22 is movably installed inside the telescopic column 24. A movable seat 19 is fixed to one end of the positioning column 22. The lifting mechanism 23 is a linear reciprocating electric push rod. The lifting mechanism 23 pushes the movable seat 19 to move up and down inside the barrel 1 through the telescopic column 24 and the positioning column 22.

[0037] In this embodiment, a buffer seat 20 is fixed on the movable seat 19. A filter plate 21 and a crossbar are fixed between the buffer seat 20 and the movable seat 19. A support mechanism 26 and a limiting post 25 are fixed to one end of the positioning post 22. One end of the support mechanism 26 is fixed inside the telescopic post 24. A discharge port 35 is opened at the bottom of the barrel 1. A discharge pipe 10 is fixed to one end of the discharge port 35. A reinforcing rod 34 is welded to the inner wall of the discharge port 35. One end of the reinforcing rod 34 is fixed to one end of the support post 18. The support mechanism 26 is a support spring. The support mechanism 26 contracts when the movable seat 19 is pressed downward in the later stage. After contraction, a rebound force is generated. The rebound force pushes the movable seat 19 to return to its original position, thereby facilitating the reciprocating movement of the movable seat 19.

[0038] A method for extracting lutein from marine microalgae using the device described in claim 1, the method comprising the following steps:

[0039] Step 1: Raw material preparation;

[0040] Step 2: Cell wall breaking and pulverization. Enzymatic cell wall breaking is performed using a compound enzyme, followed by the use of a hypotonic buffer solution to swell the cell contents, and then ultrasonic waves are used to assist in the breaking process to improve the cell wall breaking effect.

[0041] Step 3: Optimize extraction. Use ethanol as a carrier and optimize extraction conditions using uniform design. Detect and identify the lutein content in the extract using HPLC and HPLC-MS, and calculate the extraction rate.

[0042] Step 4: Wash and collect with water;

[0043] Step 5: Crystallization extraction. Collect the lutein components and finally refine them using recrystallization technology to obtain pure lutein. Detect the content and calculate the extraction rate.

[0044] In this embodiment, in step four, column chromatography is used to separate and purify lutein. Acetone is used to extract the total pigments from the disrupted Chlorella cells. Then, polystyrene-divinylbenzene reversed-phase resin is used for reversed-phase chromatography to separate lutein from chlorophyll and other carotenoids. The solvent ratio of the hexane-methanol-water elution system is determined, and gradient elution is performed. In step one, a chlorophyll fluorescence imaging system is used to rapidly and efficiently screen microalgae with strong chlorophyll fluorescence. The preliminarily screened microalgae are then cultured on a large scale, and the lutein content in the algal cells is accurately analyzed using high-performance liquid chromatography. Finally, high-quality microalgae with high lutein content are screened.

[0045] Working principle: During use, the liquid raw material enters the interior of the ring pipe 27 through the feed pipe 8. The liquid raw material inside the ring pipe 27 enters the interior of the spray seat 29 through the connecting pipe 28. The liquid raw material inside the spray seat 29 falls downward through the opening 30. The liquid moves downward on the surface of the fixed pipe 12. The ultrasonic generator 31 is activated to generate ultrasonic waves, which break down the cell walls in the liquid. The broken liquid raw material enters the interior of the tank 1. The drive motor 7 is activated to drive the rotating column 14 to rotate. The rotation of the rotating column 14 causes the stirring column 15 to stir the liquid raw material left and right. At the same time as the rotating column 14 rotates, the transmission seat 16 rotates synchronously. When the transmission seat 16 rotates, the driven gear 32 on the stirring frame 17 rotates on the driving bevel gear 33. The stirring frame 17 rotates up and down under the rotation of the driven gear 32. The mixing column 15 works in conjunction with the stirring column to achieve multi-directional stirring of the liquid while accelerating the wall breaking through the liquid collision. During the wall breaking process, additives are added later through the spray seat 29. The additives flow through the opening 30 to different positions to facilitate rapid mixing later. After the wall breaking is completed, the lifting mechanism 23 is activated to push the telescopic column 24 and the positioning column 22 upward. The positioning column 22 pushes the movable seat 19 upward. After the movable seat 19 moves upward, the stirring frame 17 rotates downward and contacts the buffer seat 20. Under the push of the stirring frame 17, the buffer seat 20 pushes the movable seat 19 downward. The movable seat 19 shakes up and down, causing the impurities on the surface of the filter plate 21 to shake up and down and be discharged from the filter holes, thereby effectively avoiding clogging. During filtration, the discharge pipe 10 is opened, and the liquid inside the tank 1 is discharged and collected after being filtered through the filter plate 21. After the liquid raw material is discharged outward, it is further purified and extracted later.

[0046] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. It will be apparent to those skilled in the art that the invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other specific forms without departing from its spirit or essential characteristics. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, all variations falling within the meaning and scope of equivalents of the claims are intended to be included within the scope of the invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

[0047] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. An extraction device for lutein from marine microalgae, comprising a barrel (1), characterized in that: A fixed seat (2) is fixed on the side of the barrel (1). A fixed column (3) is welded to the top of the fixed seat (2). A fixed block (4) is movably installed on the top of the fixed column (3). A cover plate (5) is fixed on one side of the fixed block (4). A fixed ring box (6) and a drive motor (7) are fixed on the cover plate (5). A stirring assembly (13) is installed on the drive motor (7). The stirring assembly (13) is movably installed inside the barrel (1). A fixed pipe (12) is fixed on the inner wall of the cover plate (5). A spray seat (29) is fixed on the fixed pipe (12). A connecting pipe (28) is fixed on the spray seat (29). A ring pipe (27) is fixed between the connecting pipes (28). A feed pipe (8) is fixed on the ring pipe (27).

2. The extraction device for lutein from marine microalgae according to claim 1, characterized in that: The bottom of the spray seat (29) has an opening (30), an ultrasonic generator (31) is fixedly installed inside the fixed tube (12), a connecting seat (9) is fixed on the side of the barrel (1), and a support frame (11) is fixedly installed on the connecting seat (9).

3. The extraction device for lutein from marine microalgae according to claim 1, characterized in that: The stirring assembly (13) includes a rotating column (14), one end of which is fixed to a drive motor (7), a stirring column (15) is welded onto the rotating column (14), and a transmission seat (16) is fixed to one end of the rotating column (14).

4. The extraction device for lutein from marine microalgae according to claim 3, characterized in that: A stirring rack (17) is movably installed inside the transmission base (16). A support column (18) is movably installed at the bottom of the transmission base (16). A driving bevel gear (33) is fixed at one end of the support column (18). A driven gear (32) is rotatably installed on the side of the driving bevel gear (33). The driven gear (32) is fixed at one end of the stirring rack (17).

5. The extraction device for lutein from marine microalgae according to claim 1, characterized in that: The bottom of the barrel (1) is fixed with a lifting mechanism (23), and a telescopic column (24) is installed on the lifting mechanism (23). A positioning column (22) is movably installed inside the telescopic column (24), and a movable seat (19) is fixed at one end of the positioning column (22).

6. The extraction device for lutein from marine microalgae according to claim 5, characterized in that: A buffer seat (20) is fixed on the movable seat (19). A filter plate (21) and a crossbar are fixed between the buffer seat (20) and the movable seat (19). A support mechanism (26) and a limiting column (25) are fixed at one end of the positioning column (22). One end of the support mechanism (26) is fixed inside the telescopic column (24).

7. The extraction device for lutein from marine microalgae according to claim 1, characterized in that: The bottom of the barrel (1) has a discharge port (35), one end of which is fixed with a discharge pipe (10), and a reinforcing rod (34) is welded on the inner wall of the discharge port (35). One end of the reinforcing rod (34) is fixed to one end of the support column (18).

8. A method for extracting lutein from marine microalgae using the apparatus described in claim 1, characterized in that: The method includes the following steps: Step 1: Raw material preparation; Step 2: Cell wall breaking and pulverization. Enzymatic cell wall breaking is performed using a compound enzyme, followed by the use of a hypotonic buffer solution to swell the cell contents, and then ultrasonic waves are used to assist in the breaking process to improve the cell wall breaking effect. Step 3: Optimize extraction. Use ethanol as a carrier and optimize extraction conditions using uniform design. Detect and identify the lutein content in the extract using HPLC and HPLC-MS, and calculate the extraction rate. Step 4: Wash and collect with water; Step 5: Crystallization extraction. Collect the lutein components and finally refine them using recrystallization technology to obtain pure lutein. Detect the content and calculate the extraction rate.

9. The method for extracting lutein from marine microalgae according to claim 8, characterized in that: In step four, column chromatography is used to separate and purify lutein. Acetone is used to extract the total pigments from the broken-cell walls of Chlorella cells. Then, polystyrene-divinylbenzene type reversed-phase resin is used for reversed-phase chromatography to separate lutein from chlorophyll and other carotenoids. The solvent ratio of the hexane-methanol-water elution system is determined, and gradient elution is performed.

10. The method for extracting lutein from marine microalgae according to claim 8, characterized in that: In step one, a chlorophyll fluorescence imaging system is used to rapidly and efficiently screen microalgae with strong chlorophyll fluorescence. The microalgae to be identified in the preliminary screening are then cultured in a larger scale, and the lutein content in the algal cells is accurately analyzed using high-performance liquid chromatography. Finally, high-quality microalgae with high lutein content are screened out.

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