Method for removing ammonia nitrogen in water body by coupling periphyton with manganese under aerobic condition

Abstract

The invention belongs to the technical field of methods for removing ammonia nitrogen in a water body, and particularly relates to a method for removing ammonia nitrogen in a water body by coupling periphyton with manganese under an aerobic condition, in the ammonia nitrogen removal process, the initial ammonia nitrogen concentration in the water body is 10mg / L, in view of the adsorption characteristic of periphyton, the ammonia nitrogen in part of the water body is adsorbed, and the ammonia nitrogen in part of the water body is removed. And finally, after the experiment is finished, the ammonia nitrogen content is stabilized at about 8.2 mg / L, and the removal rate is 18%. After the manganese coupling periphyton is put, the ammonia nitrogen content in the water body is rapidly reduced in the whole process and seems to have a reduction space, the concentration of the ammonia nitrogen in the water body is 3.72 mg / L after the test is finished, and the removal rate is 62.8%; compared with the prior art, the common periphyton is slow in removal speed and low in removal rate.

Description

Technical Field

[0001] This invention belongs to the technical field of methods for removing ammonia nitrogen from water bodies, and particularly relates to a method for removing ammonia nitrogen from water bodies by coupling manganese with periphyte organisms under aerobic conditions. Background Technology

[0002] Excessive accumulation of ammonia nitrogen in water bodies is one of the most significant problems currently facing water environment management. Ammonia nitrogen not only leads to eutrophication and ecological disasters such as cyanobacterial blooms, but also transforms into nitrite and nitrate through nitrification, posing a serious threat to the survival of aquatic organisms and accumulating in the food chain, thus affecting human health. Currently, mainstream ammonia nitrogen removal technologies include physical adsorption, chemical oxidation, and biological treatment. Among these, biological treatment is widely used in practical engineering due to its lower cost and environmental friendliness. However, traditional biological treatment technologies rely on the metabolic activities of specific functional microorganisms, making them susceptible to fluctuations in environmental factors such as dissolved oxygen, temperature, and pH. This results in unstable treatment efficiency and significant performance degradation under low-temperature conditions, making it difficult to meet the high-efficiency nitrogen removal requirements in complex aquatic environments.

[0003] Manganese, a common variable-valence metal element in nature, has oxides with strong redox activity and adsorption properties, and is gradually gaining attention in the field of water pollutant removal. While existing research attempts to combine manganese-based materials with biological treatment technologies to improve ammonia nitrogen removal, it primarily focuses on manganese reduction coupled with denitrification under anaerobic or anoxic conditions, with insufficient exploration of the synergistic mechanism between manganese and microorganisms in aerobic environments. Furthermore, traditional coupling systems often involve the direct addition of manganese salts or manganese oxide powders, which can easily lead to excessive manganese ion dissolution and secondary pollution risks. Additionally, the bonding stability between microorganisms and manganese-based materials is poor, making it difficult to form a sustained and efficient synergistic denitrification system, thus limiting the large-scale application of this technology in actual ammonia nitrogen treatment in water bodies. Summary of the Invention

[0004] The purpose of this invention is to address the aforementioned technical problems by providing a method for removing ammonia nitrogen from water bodies using perennial biological coupling with manganese under aerobic conditions.

[0005] In view of this, the present invention provides a method for removing ammonia nitrogen from water bodies by coupling manganese with periphyte organisms under aerobic conditions, comprising the following steps: Step 1: Using mature peritrichous organisms in conventional WC culture medium in the laboratory as the manganese coupling target, prepare manganese-peritrichous organism couplers; In step one, the mature peritrichous organisms are those that appear dark green in WC culture medium and are attached to a carrier or inner wall in the WC culture medium; the preparation process of the manganese-peritrichous coupling body is as follows: Gently peel off the mature pericarp using a sterilized silicone scraper. After removing the peeled pericarp from WC culture medium using a 100-mesh filter and draining the surface water, transfer it to manganese coupling culture medium for a 7-day manganese coupling process. Step 2: Transfer the manganese-coupled periwinkle to a water body containing ammonia nitrogen to carry out the ammonia nitrogen removal reaction, and regularly monitor the required indicators.

[0006] Preferably, the manganese-coupled culture medium in step one comprises: NaNO3 85-90 mg·L -1 CaCl2·2H2O 3.60-4.0 mg·L -1 MgSO4·7H2O 36-40 mg·L -1 NaHCO3 12-15 mg·L -1 NaSiO4·9H2O 28-30 mg·L -1 H3BO3 24-25 mg·L -1 MnCl2·4H2O 70-75 mg·L -1 Na2EDTA·2H2O 4-5 mg·L -1 ZnSO4·7H2O 22-25 mg·L -1 CuSO4·5H2O 2-3 mg·L -1 NaMoO4·2H2O 6-8 mg·L -1 Na3VO4 18-20 mg·L -1 VB 12 1 ml·L solution -1 1 ml / L of VB1 solution -1 Biotin solution 1 ml·L -1 The solvent is 1L of distilled water; VB 12 Solution composition: HEPES buffer 12 g·L -1 VB 12 0.135 g·L -1 ; Composition of VB1 solution: HEPES buffer 12 g·L -1 VB 12 0.335 g·L -1 ; Composition of biotin solution: HEPES buffer 12 g·L -1 Biotin 0.025 g·L -1 ; The three vitamin solutions were prepared in 200 mL of HEPES buffer, the pH was adjusted to 7.8, and the solutions were stored at low temperature and protected from light.

[0007] Preferably, in step one, the materials used to prepare the conventional WC culture medium are: NaNO3 85-90mg·L-1, CaCl2·2H2O 3.60-4.0mg·L -1 MgSO4·7H2O 36-40 mg·L -1 NaHCO3 12-15 mg·L -1 NaSiO4·9H2O 28-30 mg·L -1 H3BO3 24-25 mg·L -1 MnCl2·4H2O 180-200 μg·L -1 FeCl3·6H2O 3-4 mg·L -1 Na2EDTA·2H2O 4-5 mg·L -1 ZnSO4·7H2O 22-25 mg·L -1 CuSO4·5H2O 2-3 mg·L -1 NaMoO4·2H2O 6-8 mg·L -1 Na3VO4 18-20 mg·L -1 VB 12 1 ml·L solution -1 1 ml / L of VB1 solution -1 Biotin solution 1 ml·L -1 The solvent is 1L of distilled water; Composition of VB12 solution: HEPES buffer 12 g·L -1 VB 12 0.135 g·L -1 ; Composition of VB1 solution: HEPES buffer 12 g·L -1 VB 12 0.335 g·L -1 ; Composition of biotin solution: HEPES buffer 12 g·L -1 Biotin 0.025 g·L -1 ; The three vitamin solutions were prepared in 200 mL of HEPES buffer, the pH was adjusted to 7.8, and the solutions were stored at low temperature and protected from light.

[0008] Preferably, the manganese coupling reaction process described in step one is carried out in an intelligent light incubator with a light intensity of 10,000-12,000 LUX and a light / dark time of 12 / 12 h. The incubation temperature is 25±2℃. Seal the container opening with polyethylene film and poke 10-15 small holes. Replenish the culture system with manganese-coupled culture medium every 3 days to maintain the initial volume; After manganese coupling was completed, a portion of the pericarp sample was centrifuged, dried, and the valence state of manganese on the surface was detected.

[0009] Preferably, in step one, the centrifugation temperature is 25±1℃, the rotation speed is 7000-8000rpm, the time is 8-10min, and the drying temperature is 40℃.

[0010] Preferably, in step S2, a 100-mesh filter is used to transfer the manganese-coupled periphytes to a water body containing ammonia nitrogen.

[0011] Preferably, the solid-liquid ratio of the pericarp to the manganese coupling solution in step S1 is 25g:1L; the concentration of Mn(II) ions or the amount of pericarp added can be appropriately increased as needed, but the manganese saturation of the pericarp must be taken into account.

[0012] Preferably, in step S1, the concentration of added Mn(II) is appropriately adjusted based on the traditional culture method according to different types of periwinkle organisms, and the final manganese valence state formed in the coupling system is used as the basis for determining whether the coupling is successful.

[0013] Preferably, in step S2, the amount of manganese coupling dosage and the residence time of ammonia nitrogen in the system are reasonably determined based on the on-site water conditions and relevant standards.

[0014] The beneficial effects of this invention are: This method achieves efficient and stable removal of ammonia nitrogen under aerobic conditions by constructing a manganese-peripheral clump coupling system. On one hand, after manganese coupling, the peripheral clumps generate manganese oxides such as MnO and MnO2 on their surface. The redox activity of manganese synergizes with the biological metabolic functions of the peripheral clumps, significantly reducing the impact of ammonia nitrogen release from the peripheral clumps on the water body during the initial stage of ammonia nitrogen removal, and also significantly accelerating the ammonia nitrogen removal rate. Compared with ordinary peripheral clumps, the ammonia nitrogen removal process is faster and tends to stabilize in the later stages, effectively solving the problems of low efficiency and susceptibility to environmental interference in traditional biological treatment methods. On the other hand, the coupling system can maintain stable operation under natural aerobic conditions without the need for strict control or maintenance of specific anaerobic / anoxic environments. The dissolved oxygen in the water body always meets the requirements of an aerobic environment, simplifying the operation process, reducing engineering maintenance costs, and providing a more convenient technical path for ammonia nitrogen treatment in complex water environments.

[0015] Meanwhile, this method boasts excellent environmental safety and applicability. During the manganese coupling process, periphytes exhibit stable absorption and conversion capabilities for manganese ions, with the manganese removal rate consistently maintained between 86% and 93%. This significantly reduces the residual manganese concentration in the water, avoiding the adverse effects of excessive manganese accumulation on periphyte activity and aquatic ecology, thus solving the problem of secondary pollution easily caused by traditional manganese-based material coupling technologies. Furthermore, by adjusting the Mn(II) addition concentration, periphyte dosage, and ammonia nitrogen retention time in the water, the method can be adapted to different types of periphytes and on-site water conditions, further broadening the application scenarios of the technology and providing a flexible and feasible solution for ammonia nitrogen removal from water bodies under different pollution levels and environmental conditions.

[0016] In the ammonia nitrogen removal process of this invention, the initial ammonia nitrogen concentration in the water is 10 mg / L. Due to the adsorption characteristics of periwinkle, some of its own ammonia nitrogen will be released into the new environment, resulting in an ammonia nitrogen content in the water exceeding 10 mg / L. After the manganese-coupled periwinkle is added, the increase in ammonia nitrogen concentration in the water is relatively small, and the ammonia nitrogen content in the water decreases rapidly in the first four days, after which the rate of decrease tends to level off. In contrast, ordinary periwinkle not only significantly increases the ammonia nitrogen concentration in the water in the initial stage, but also has a slow removal rate, resulting in a significantly lower overall treatment efficiency. Attached Figure Description

[0017] Figure 1 -Elemental XPS image of the surface of the manganese-peripheral biocouple; Figure 2 - Changes in the concentration of ammonia nitrogen in water bodies; Figure 3 -DO concentration change graph; Figure 4 - Differences in the abundance of genes involved in ammonia nitrogen uptake; Figure 5 - The removal efficiency of basal organisms under different Mn concentrations. Detailed Implementation

[0018] The technical solutions of the embodiments of this application will be clearly described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this application. All other embodiments obtained by those skilled in the art based on the embodiments of this application are within the scope of protection of this application.

[0019] It should be noted that all directional and positional terms used in this invention, such as "up," "down," "left," "right," "front," "back," "vertical," "horizontal," "inner," "outer," "top," "lower," "lateral," "longitudinal," and "center," are only used to explain the relative positional relationships and connections between components in a specific state (as shown in the accompanying drawings). They are merely for the convenience of describing the invention and do not require the invention to be constructed and operated in a specific orientation; therefore, they should not be construed as limitations on the invention. Furthermore, descriptions involving "first," "second," etc., are for descriptive purposes only and should not be construed as indicating or implying their relative importance or implicitly specifying the number of technical features indicated.

[0020] In the description of this invention, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal communication between two components. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0021] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "illustrative embodiment," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0022] The technical solution used in the embodiments includes the following steps: Step S1: Using mature peritrichous organisms in conventional WC culture medium in the laboratory as the manganese coupling object, prepare manganese-peritrichous organism couplers.

[0023] Mature peritrichous organisms that appear dark green in the WC culture medium and are attached to the carrier or inner wall of the WC culture medium are gently peeled off with a sterilized silicone scraper. The peeled peritrichous organisms are then scooped out of the WC culture medium using a 100-mesh filter and drained of surface water. They are then transferred to manganese coupling culture medium for a 7-day manganese coupling process.

[0024] The manganese-coupled culture medium consists of: NaNO3 85-90 mg·L⁻¹ -1 CaCl2·2H2O 3.60-4.0 mg·L -1MgSO4·7H2O 36-40 mg·L -1 NaHCO3 12-15 mg·L -1 NaSiO4·9H2O 28-30 mg·L -1 H3BO3 24-25mg·L -1 MnCl2·4H2O 70-75 mg·L -1 Na2EDTA·2H2O 4-5 mg·L -1 ZnSO4·7H2O 22-25 mg·L -1 CuSO4·5H2O 2-3 mg·L -1 NaMoO4·2H2O 6-8 mg·L -1 Na3VO4 18-20 mg·L -1 VB 12 1 ml·L solution -1 1 ml / L of VB1 solution -1 Biotin solution 1 ml·L -1 The solvent is 1L of distilled water.

[0025] VB 12 Solution composition: HEPES buffer 12 g·L -1 VB 12 0.135 g·L -1 ; Composition of VB1 solution: HEPES buffer 12 g·L -1 VB 12 0.335 g·L -1 ; Composition of biotin solution: HEPES buffer 12 g·L -1 Biotin 0.025 g·L -1 ; The three vitamin solutions were prepared in 200 mL of HEPES buffer, the pH was adjusted to 7.8, and the solutions were stored at low temperature and protected from light.

[0026] The materials used to prepare the conventional WC culture medium are: NaNO3 85-90 mg·L⁻¹, CaCl₂·2H₂O 3.60-4.0 mg·L⁻¹. -1 MgSO4·7H2O 36-40 mg·L -1 NaHCO3 12-15 mg·L -1 NaSiO4·9H2O 28-30 mg·L -1 H3BO3 24-25 mg·L -1 MnCl2·4H2O 180-200μg·L-1 FeCl3·6H2O 3-4 mg·L -1 Na2EDTA·2H2O 4-5 mg·L -1 ZnSO4·7H2O 22-25 mg·L -1 CuSO4·5H2O 2-3 mg·L -1 NaMoO4·2H2O 6-8 mg·L -1 Na3VO4 18-20 mg·L -1 VB 12 1 ml·L solution -1 1 ml / L of VB1 solution -1 Biotin solution 1 ml·L -1 The solvent is 1L of distilled water.

[0027] Composition of VB12 solution: HEPES buffer 12 g·L -1 VB 12 0.135 g·L -1 ; Composition of VB1 solution: HEPES buffer 12 g·L -1 VB 12 0.335 g·L -1 ; Composition of biotin solution: HEPES buffer 12 g·L -1 Biotin 0.025 g·L -1 ; The three vitamin solutions were prepared in 200 mL of HEPES buffer, the pH was adjusted to 7.8, and the solutions were stored at low temperature and protected from light.

[0028] The entire manganese coupling reaction was carried out in an intelligent light-illuminated incubator with a light intensity of 10,000-12,000 LUX and a light / dark cycle of 12 / 12 h. The incubation temperature was 25±2℃. The container opening was sealed with a polyethylene film and 10-15 small holes were punched to prevent contamination of the sample by external microorganisms and to reduce losses of the culture medium through evaporation. The manganese coupling culture medium was replenished to the culture system every 3 days to maintain the initial volume. After manganese coupling was completed, a portion of the pericarp sample was centrifuged, dried, and the valence state of surface manganese was determined.

[0029] Step S2: Removal of ammonia nitrogen by manganese-peripheral clumps: The peripheral clumps that have been coupled with manganese are transferred to a water body containing ammonia nitrogen through a filter screen (100 mesh) to carry out the ammonia nitrogen removal reaction, and the required indicators are tested regularly.

[0030] In practice, in step S1, the concentration of added Mn(II) should be appropriately adjusted based on the traditional culture method according to different types of periphytes, and the final manganese valence state formed in the coupling system should be used as the basis for determining whether the coupling is successful.

[0031] In specific implementation, the solid-liquid ratio of the periwinkle to the manganese coupling solution in step S1 is 25 g: 1 L. The Mn(II) ion concentration or the amount of periwinkle added can be appropriately increased as needed to enhance the removal effect on ammonia nitrogen. However, since manganese is a heavy metal, the manganese saturation of the periwinkle should be considered when adjusting the concentration to avoid excessive accumulation that could adversely affect its activity and the aquatic ecosystem.

[0032] In specific implementation, in step S1, the centrifugation temperature is 25±1℃, the rotation speed is 7000-8000rpm, the time is 8-10min, and the drying temperature is 40℃.

[0033] In specific implementation, in step S2, the removal capacity of different types of periwinkle organisms for ammonia nitrogen varies in terms of maximum removable concentration and required reaction time. The amount of manganese coupling dosage and the residence time of ammonia nitrogen in the system should be reasonably determined in combination with the on-site water conditions and relevant standards to ensure treatment effect and stability.

[0034] Example 1: In Example 1, X-ray spectroscopy (XPS) analysis was performed on the surface manganese elements of the periphyte after manganese coupling treatment in step S1 above and ordinary periphyte without manganese coupling treatment to compare the presence state of manganese elements on the surface of the two organisms.

[0035] The obtained XPS plot was opened using Avantage software and the elements were labeled. Then, the two sets of data were merged and compared using Origin 2019b software. Figure 1 It was determined that the surface of periclump organisms coupled with manganese exhibits the formation of manganese oxides, namely MnO and MnO2.

[0036] Example 2: Example 2 compares the changes in ammonia nitrogen content in water during the removal of ammonia nitrogen from water by the manganese-peripheral co-coupled organism and ordinary peripheral organism in step S2 above.

[0037] During ammonia nitrogen removal, the initial ammonia nitrogen concentration in the water was 10 mg / L. Due to the adsorption characteristics of *Periplaneta americana*, some of the ammonia nitrogen in the water was adsorbed, causing the ammonia nitrogen content in the water to decrease rapidly and then stabilize. After the experiment, the concentration stabilized at around 8.2 mg / L, with a removal rate of 18%. After the addition of manganese-coupled *Periplaneta americana*, the ammonia nitrogen content in the water not only decreased rapidly throughout the process but also seemed to have room for further reduction. After the experiment, the ammonia nitrogen concentration in the water was 3.72 mg / L, with a removal rate of 62.8%. In contrast, ordinary *Periplaneta americana* not only had a slow removal speed but also a low removal rate. Figure 2 ).

[0038] Example 3: Example 3 compares the changes in dissolved oxygen (DO) content in water during the removal of ammonia nitrogen from water by the manganese-peripheral co-coupled organism and ordinary peripheral organism in step S2 above.

[0039] Dissolved organic matter (DO) in water is an important indicator for determining the redox state and metabolic type of microorganisms. Generally, a DO concentration ≥ 2.0 mg / L in water can be considered an aerobic environment. Both manganese-coupled and ordinary peritrichous organisms showed DO levels greater than 2.0 mg / L during ammonia nitrogen removal, indicating that the experimental conditions were aerobic, and no strict control or maintenance of specific anaerobic / anoxic conditions was implemented. Under these conditions, peritrichous organisms primarily relied on their own characteristics and related nitrogen transformation processes to remove ammonia nitrogen, while the manganese-coupled treatment group exhibited a higher removal rate and stability. This demonstrates that it can still fully leverage its synergistic adsorption and biotransformation advantages under natural aerobic conditions, providing a feasible basis for simplifying operating conditions and reducing engineering maintenance costs. Figure 3 ).

[0040] Example 4: Example 4 compares the changes in the abundance of nitrogen fixation genes in the manganese-peripheral plexiform coupler and the nitrogen metabolism pathway of ordinary peripheral plexiforms in step S2 above.

[0041] Among ammonia nitrogen absorption and utilization genes, NirB and GltD genes are two of the most representative functional genes, encoding the large subunit of nitrite reductase and the small subunit of glutamate synthase, respectively, directly involved in nitrogen reduction and assimilation processes. Experimental results showed that the abundance of NirB and GltD genes was significantly increased in manganese-coupled periwinkle organisms, indicating that the addition of manganese promoted the enrichment and enhanced activity of related nitrogen-cycling functional bacterial communities, thereby improving the microbial absorption and utilization capacity of ammonia nitrogen and accelerating its biotransformation and removal. In contrast, the abundance of these two genes was lower in ordinary periwinkle organisms, indicating relatively insufficient ammonia nitrogen absorption and utilization function, resulting in lower ammonia nitrogen removal efficiency. Figure 4 ).

[0042] Example 5: Example 5 involves the heavy metal manganese. It supplements the environmental safety verification experiment with different manganese concentrations after coupling with *Periplaneta americana*. The results are as follows: Figure 5 As shown in the figure, the manganese removal rate remained consistently between approximately 86% and 93%, indicating that *Cyclocarya paliurus* possesses a stable removal capacity for manganese concentrations at various levels. This result demonstrates that *Cyclocarya paliurus* can not only adapt to different manganese loading conditions but also significantly reduce the residual concentration of manganese in water bodies, thereby achieving the harmless treatment of heavy metal manganese and exhibiting good environmental safety and application potential.

[0043] The embodiments of this application have been described above with reference to the accompanying drawings. Unless otherwise specified, the embodiments and features in the embodiments of this application can be combined with each other. This application is not limited to the specific embodiments described above. The specific embodiments described above are merely illustrative and not restrictive. Those skilled in the art can make many other forms under the guidance of this application without departing from the spirit and scope of the claims, and all of these forms are within the protection scope of this application.

Claims

1. A method for removing ammonia nitrogen from water bodies by coupling manganese with periphyte organisms under aerobic conditions, characterized in that: Includes the following steps: Step 1: Using mature peritrichous organisms in conventional WC culture medium in the laboratory as the manganese coupling target, prepare manganese-peritrichous organism couplers; In step one, the mature peritrichous organisms are those that appear dark green in WC culture medium and are attached to a carrier or inner wall in the WC culture medium; the preparation process of the manganese-peritrichous coupling body is as follows: Gently peel off the mature pericarp using a sterilized silicone scraper. After removing the peeled pericarp from WC culture medium using a 100-mesh filter and draining the surface water, transfer it to manganese coupling culture medium for a 7-day manganese coupling process. Step 2: Transfer the manganese-coupled periwinkle to a water body containing ammonia nitrogen to carry out the ammonia nitrogen removal reaction, and regularly monitor the required indicators.

2. The method for removing ammonia nitrogen from water bodies under aerobic conditions using periphytocopherol coupled with manganese, as described in claim 1, is characterized in that: The components of the manganese coupling culture medium mentioned in step one are: NaNO3 85-90 mg·L -1 CaCl2·2H2O 3.60-4.0 mg·L -1 MgSO4·7H2O 36-40 mg·L -1 NaHCO3 12-15 mg·L -1 NaSiO4·9H2O 28-30 mg·L -1 H3BO3 24-25 mg·L -1 MnCl2·4H2O 70-75 mg·L -1 Na2EDTA·2H2O 4-5 mg·L -1 ZnSO4·7H2O 22-25 mg·L -1 CuSO4·5H2O 2-3 mg·L -1 NaMoO4·2H2O 6-8 mg·L -1 Na3VO4 18-20 mg·L -1 VB 12 1 ml·L solution -1 1 ml / L of VB1 solution -1 Biotin solution 1 ml·L -1 The solvent is 1L of distilled water; VB 12 Solution composition: HEPES buffer 12 g·L -1 VB 12 0.135 g·L -1 ; Composition of VB1 solution: HEPES buffer 12 g·L -1 VB 12 0.335 g·L -1 ; Composition of biotin solution: HEPES buffer 12 g·L -1 Biotin 0.025 g·L -1 ; The three vitamin solutions were prepared in 200 mL of HEPES buffer, the pH was adjusted to 7.8, and the solutions were stored at low temperature and protected from light.

3. The method for removing ammonia nitrogen from water bodies under aerobic conditions using periphytocopherol coupled with manganese, as described in claim 1, is characterized in that: In step one, the materials used to prepare the conventional WC culture medium are: NaNO3 85-90mg·L-1, CaCl2·2H2O 3.60-4.0mg·L -1 MgSO4·7H2O 36-40 mg·L -1 NaHCO3 12-15 mg·L -1 NaSiO4·9H2O 28-30 mg·L -1 H3BO3 24-25 mg·L -1 MnCl2·4H2O 180-200 μg·L -1 FeCl3·6H2O 3-4 mg·L -1 Na2EDTA·2H2O 4-5 mg·L -1 ZnSO4·7H2O 22-25 mg·L -1 CuSO4·5H2O 2-3 mg·L -1 NaMoO4·2H2O 6-8 mg·L -1 Na3VO4 18-20 mg·L -1 VB 12 1 ml·L solution -1 1 ml / L of VB1 solution -1 Biotin solution 1 ml·L -1 The solvent is 1L of distilled water; Composition of VB12 solution: HEPES buffer 12 g·L -1 VB 12 0.135 g·L -1 ; Composition of VB1 solution: HEPES buffer 12 g·L -1 VB 12 0.335 g·L -1 ; Composition of biotin solution: HEPES buffer 12 g·L -1 Biotin 0.025 g·L -1 ; The three vitamin solutions were prepared in 200 mL of HEPES buffer, the pH was adjusted to 7.8, and the solutions were stored at low temperature and protected from light.

4. The method for removing ammonia nitrogen from water bodies under aerobic conditions using periphytocopherol coupled with manganese, as described in claim 1, is characterized in that: The manganese coupling reaction process described in step one is carried out in an intelligent light incubator with a light intensity of 10,000-12,000 LUX and a light / dark time of 12 / 12 h. The incubation temperature is 25±2℃. Seal the container opening with polyethylene film and poke 10-15 small holes. Replenish the culture system with manganese-coupled culture medium every 3 days to maintain the initial volume; After manganese coupling was completed, a portion of the pericarp sample was centrifuged, dried, and the valence state of manganese on the surface was detected.

5. The method for removing ammonia nitrogen from water bodies under aerobic conditions using periphytocopherol coupled with manganese, as described in claim 4, is characterized in that: In step one, the centrifugation temperature is 25±1℃, the rotation speed is 7000-8000rpm, the time is 8-10min, and the drying temperature is 40℃.

6. The method for removing ammonia nitrogen from water bodies under aerobic conditions using periphytocopherol coupled with manganese, as described in claim 1, is characterized in that: In step S2, a 100-mesh filter is used to transfer the manganese-coupled periphytes to a water body containing ammonia nitrogen.

7. The method for removing ammonia nitrogen from water bodies under aerobic conditions using periphytocopherol coupled with manganese, as described in claim 1, is characterized in that: In step S1, the solid-liquid ratio of the pericarp to the manganese coupling solution is 25 g: 1 L. The concentration of Mn(II) ions or the amount of pericarp added can be appropriately increased as needed, but the manganese saturation of the pericarp must be taken into account.

8. The method for removing ammonia nitrogen from water bodies under aerobic conditions using periphytocopherol coupled with manganese, as described in claim 1, is characterized in that: In step S1, the concentration of Mn(II) is appropriately adjusted based on the traditional culture method according to different types of periwinkle organisms, and the final manganese valence state formed in the coupling system is used as the basis for determining whether the coupling is successful.

9. The method for removing ammonia nitrogen from water bodies under aerobic conditions using periphytocopherol coupled with manganese, as described in claim 1, is characterized in that: In step S2, the amount of manganese coupling dosage and the residence time of ammonia nitrogen in the system are reasonably determined based on the on-site water conditions and relevant standards.

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