Dandelion polysaccharide with high hypoglycemic activity as well as preparation method and application thereof

By extracting, separating, and purifying the whole plant of dandelion, a dandelion polysaccharide DPH2c with high hypoglycemic activity was prepared, which solved the problem of insufficient purification of dandelion polysaccharides and achieved significant antioxidant and hypoglycemic effects, which can be applied to the preparation of hypoglycemic drugs and functional foods.

CN121108376APending Publication Date: 2025-12-12CHANGZHOU UNIV
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Patent Information

Application Number
CN202511243527.1
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-09-02
Publication Date
2025-12-12

AI Technical Summary

Technical Problem

The current purification level of dandelion polysaccharides is insufficient, and the complexity and uncertainty of their composition limit the in-depth development and application of their medicinal value. Furthermore, existing drugs have problems such as adverse reactions or high costs when treating diabetes.

Method used

A relatively homogeneous polysaccharide DPH2c with high hypoglycemic activity was extracted and purified from the whole plant of dandelion using hot water extraction, fractional alcohol precipitation, deproteinization, DEAE-52 ion exchange column chromatography and Sephadex G-200 column chromatography. It was screened for antioxidant, α-amylase and α-glucosidase inhibitory activities and used to prepare hypoglycemic drugs.

Benefits of technology

A dandelion polysaccharide DPH2c with good antioxidant capacity and inhibitory activity against α-amylase and α-glucosidase was prepared, which significantly improved insulin resistance and reduced blood glucose levels. It can be applied to the preparation of antioxidant and hypoglycemic drugs and functional foods.

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Abstract

The invention discloses dandelion polysaccharide with high hypoglycemic activity as well as a preparation method and application thereof. The preparation method comprises the following steps: drying, crushing and sieving whole dandelion plants, degreasing and decolorizing, extracting and concentrating the obtained pretreated powder by hot water, carrying out graded alcohol precipitation to obtain crude dandelion polysaccharide, screening the crude dandelion polysaccharide with optimal activity by virtue of oxidation resistance and in-vitro blood sugar reduction, deproteinizing, and separating and purifying by virtue of DEAE-52 and Sephadex G-200 to obtain relatively uniform dandelion polysaccharide. The dandelion polysaccharide can remarkably inhibit the activity of alpha-amylase and alpha-glucosidase, improve the glucose consumption capacity of IR-HepG2 cells (an insulin resistance model) and effectively reduce fasting blood glucose of type 2 diabetes mellitus mice, can be used for developing and preparing hypoglycemic products, and meanwhile, can be used for preparing hypoglycemic products. The fine structure characteristics of the dandelion polysaccharide with the hypoglycemic activity are further analyzed, and reference and data support are provided for development and application of dandelion and polysaccharide.
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