Liquid chromatographic analysis system

Abstract

The invention discloses a liquid chromatographic analysis system. The liquid chromatography system includes: a center cleavage flow path configured to preliminarily divide a sample; the secondary cutting flow paths are arranged in parallel; the path switching assembly is connected with the central cutting flow path and the at least two secondary cutting flow paths; and the control unit is in communication connection with the path switching assembly and is used for controlling the path switching assembly so as to guide the at least two types of target cutting fractions divided by the central cutting flow path into different secondary cutting flow paths in a parallel or alternate manner. Through the mode, the liquid chromatographic analysis system provided by the invention improves the analysis speed and the overall flux of a plurality of target cutting components in a complex sample, combines the advantages of a center cutting technology, and improves the separation efficiency through the primary separation of the center cutting flow path and the fine separation of each secondary cutting flow path. The separation degree and the detection accuracy of a target analyte in a complex sample matrix are effectively improved, and a cutting path can be flexibly selected.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of sample analysis equipment, in particular to a liquid chromatography analysis system. BACKGROUND

[0002] Liquid chromatography (LC) is a widely used separation and analysis technique in the fields of chemistry, biology, medicine, environment, etc. Traditional liquid chromatography systems usually adopt a single-pump single-column or double-pump single-column (such as gradient elution) mode. For complex sample matrices, the separation degree of a single chromatographic column is often insufficient to effectively separate all target analytes, especially when the target and interfering substances have similar retention behaviors.

[0003] Heart-Cutting technology is one of the effective means to solve this problem. It usually uses a switching valve to cut the fraction (containing target analytes or complex components that need further separation) within a certain time period from the first chromatographic column (pre-column or main column) into the second chromatographic column (analysis column or secondary column) with different selectivity for further separation, while other components are directly discharged or enter the detector. This technology can significantly improve the separation degree and detection sensitivity of specific target substances in complex samples.

[0004] However, traditional Heart-Cutting liquid chromatography systems usually have a tandem structure, i.e., the main path and the cutting path share part of the flow path or the detector, which may result in: prolonged analysis cycle, additional time is needed for the separation and detection of the cut components on the second column, while the main path may be in a waiting state; limited throughput, for samples that require multiple cuts or contain multiple complex matrix interferences, the efficiency of tandem Heart-Cutting is not high; insufficient flexibility, it is difficult to simultaneously optimize the independent separation of multiple target cutting fractions with different properties.

[0005] To improve the analysis speed, ultra-high performance liquid chromatography (UHPLC) achieves rapid separation by using smaller particle size stationary phases and higher operating pressures, but for extremely complex sample matrices, a single UHPLC column may still face challenges. Although full two-dimensional liquid chromatography (LCxLC) can provide a large peak capacity, the instrument is complex, the analysis time is long, and the data processing is cumbersome, and not all analysis tasks require such a high amount of information. SUMMARY

[0006] The present application mainly provides a liquid chromatography analysis system to solve the problems of low analysis efficiency and insufficient throughput of liquid chromatography systems when separating complex sample matrices.

[0007] To address the aforementioned technical problems, this application provides a liquid chromatography analysis system. The liquid chromatography analysis system includes: a central cutting flow path configured for preliminary sample fractionation; at least two secondary cutting flow paths connected in parallel; a path switching component connecting the central cutting flow path and the at least two secondary cutting flow paths; and a control unit communicatively connected to the path switching component for controlling the path switching component to introduce at least two types of target fractions separated by the central cutting flow path into different secondary cutting flow paths in a parallel or alternating manner.

[0008] In some embodiments, the control unit controls the path switching component to import the initially segmented target cut fraction into the corresponding secondary cut flow path based on a preset time window.

[0009] In some embodiments, the path switching component includes at least one switching valve for controlled introduction of each of the initially separated target cut fractions into different secondary cut paths.

[0010] In some embodiments, the liquid chromatography analysis system further includes a main path detector and a waste collector; The path switching component includes a first switching valve and a second switching valve connected to each other. The first switching valve is connected to the central cutting flow path, the main path detector and the waste liquid collector, and the second switching valve is connected to each of the secondary cutting flow paths. The first switching valve is used to transport the target cut fraction output from the central cutting flow path to the second switching valve, and to transport the non-target cut fraction output from the central cutting flow path to the main detector or the waste liquid collector; the second switching valve then distributes the received target cut fraction to the corresponding secondary cutting flow path.

[0011] In some embodiments, the first switching valve is a six-way switching valve or a ten-way switching valve, and the second switching valve is a four-way switching valve or a six-way switching valve.

[0012] In some embodiments, the secondary cutting flow path includes a secondary chromatographic column and a secondary detector. The secondary chromatographic column is used to further separate the allocated target cutting fraction, and the secondary detector is used to detect the components separated by the secondary chromatographic column and generate an analytical signal. The secondary chromatographic columns in each of the secondary cutting flow paths are of different types.

[0013] In some embodiments, the secondary detector is one of an ultraviolet detector, a mass spectrometer, or a fluorescence detector.

[0014] In some embodiments, the secondary cutting flow path further includes a secondary mobile phase delivery unit, which is used to provide a corresponding mobile phase to the secondary chromatographic column and to independently control the mobile phase composition, flow rate, or gradient elution program.

[0015] In some embodiments, the central cutting flow path includes a central chromatographic column and a central mobile phase delivery unit, wherein the central chromatographic column is used for preliminary separation of the sample, and the central mobile phase delivery unit is used to provide a mobile phase to the central chromatographic column; wherein the fraction output from the central chromatographic column is selected for target cutting via the path switching component.

[0016] In some embodiments, the central cutting flow path further includes an autosampler disposed between the central chromatographic column and the central mobile phase delivery unit, the autosampler being used to automatically aspirate and inject the sample to be analyzed into the central chromatographic column.

[0017] The beneficial effects of this application are as follows: Unlike existing technologies, this application discloses a liquid chromatography analysis system. Through the synergistic effect of a central cutting flow path, a path switching component, and multiple parallel secondary cutting flow paths, the liquid chromatography analysis system avoids the waiting time associated with traditional tandem central cutting, significantly improving the analysis speed and overall throughput for multiple target cleaved components in complex samples. Simultaneously, it combines the advantages of central cutting technology, effectively improving the separation degree and detection accuracy of target analytes in complex sample matrices through preliminary separation in the central cutting flow path and fine separation in each secondary cutting flow path. This liquid chromatography analysis system can flexibly select whether to perform central cutting, the cutting time window, and which secondary cutting flow path to guide the target cleaved fraction according to the complexity of the sample and the properties of the target analyte. The secondary chromatographic column and mobile phase conditions for different secondary cutting flow paths can be independently optimized, adapting to the separation requirements of various target analytes. Attached Figure Description

[0018] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort, wherein: Figure 1 This is a schematic diagram of the structure of an embodiment of the liquid chromatography analysis system provided in this application; Figure 2 yes Figure 1 A schematic diagram of the central cut flow path of the liquid chromatography analysis system shown; Figure 3 yesFigure 1 A schematic diagram of the connection structure between the central cutting flow path and the path switching component of the liquid chromatography analysis system shown. Figure 4 yes Figure 1 The diagram shows the connection structure of each secondary cutting flow path and path switching component of the liquid chromatography analysis system. Detailed Implementation

[0019] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of this application, and not all of the embodiments. Based on the embodiments of this application, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of this application.

[0020] The terms "first," "second," and "third" used in the embodiments of this application are for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, a feature defined as "first," "second," or "third" may explicitly or implicitly include at least one of that feature. In the description of this application, "multiple" means at least two, such as two, three, etc., unless otherwise explicitly specified. Furthermore, the terms "comprising" and "having," and any variations thereof, are intended to cover non-exclusive inclusion. For example, a process, method, system, product, or device that includes a series of steps or units is not limited to the listed steps or units, but may optionally include steps or units not listed, or may optionally include other steps or units inherent to these processes, methods, products, or devices.

[0021] In this document, the term "embodiment" means that a particular feature, structure, or characteristic described in connection with an embodiment may be included in at least one embodiment of this application. The appearance of this phrase in various places throughout the specification does not necessarily refer to the same embodiment, nor is it a mutually exclusive, independent, or alternative embodiment. It will be explicitly and implicitly understood by those skilled in the art that the embodiments described herein can be combined with other embodiments.

[0022] This application provides a liquid chromatography analysis system 100, see reference. Figure 1 , Figure 1 This is a schematic diagram of the liquid chromatography analysis system provided in this application.

[0023] The liquid chromatography analysis system 100 can be widely used in fields such as drug analysis, environmental monitoring, and food safety testing, and is especially suitable for the rapid detection of trace targets in complex multi-component mixtures.

[0024] The liquid chromatography analysis system 100 includes a central cutting flow path 10, a path switching component 20, at least two secondary cutting flow paths 30, and a control unit 40. The central cutting flow path 10 is configured to perform preliminary fractionation of the sample. The secondary cutting flow paths 30 are connected in parallel. The path switching component 20 connects the central cutting flow path 10 and the at least two secondary cutting flow paths 30. The control unit 40 is communicatively connected to the path switching component 20 and is used to control the path switching component 20 to introduce at least two types of target cut fractions separated by the central cutting flow path 10 into different secondary cutting flow paths 30 in a parallel or alternating manner.

[0025] The control unit 40 can be a host computer or an embedded control system, which uses a control program to precisely control the path switching component 20, thereby ensuring the accurate switching of the target cut fraction from the central cutting flow path 10 to the corresponding secondary cutting flow path 30.

[0026] The control unit 40 also works with the central cutting flow path 10 and each secondary cutting flow path 30 to control the central cutting flow path 10 and each secondary cutting flow path 30 to complete the gradient elution process.

[0027] The central cutting flow path 10 is configured to perform preliminary fractionation of the sample, such as separating a complex mixture sample into several relatively simple fractions, so that subsequent secondary cutting flow paths 30 can perform further fine separation. The central cutting flow path 10 can use mobile phases of different polarities to achieve preliminary chromatographic separation based on the chemical characteristics of the sample.

[0028] Each secondary cutting flow path 30 can select different chromatographic columns and elution conditions according to its respective separation task, thereby achieving highly selective separation of target analytes in the target cutting fraction.

[0029] With precise control of the path switching component 20, different target cut fractions can be switched to the corresponding secondary cut flow path 30 as needed, so that multiple target cut fractions can be separated and analyzed in parallel or alternately on different paths, avoiding the waiting time of traditional tandem center cut, and significantly improving the analysis speed and overall throughput of multiple target cut components in complex samples.

[0030] The liquid chromatography analysis system 100 provided in this application combines the advantages of center cutting technology. Through the initial separation of the sample by the center cutting flow path 10 and the fine separation by each secondary cutting flow path 30, it effectively improves the separation degree and detection accuracy of target analytes in complex samples.

[0031] In addition, the liquid chromatography analysis system 100 has good scalability and can be adapted to more complex sample analysis needs by increasing the number of secondary cutting flow paths 30.

[0032] SeeFigure 2 , Figure 2 yes Figure 1 The diagram shows the structure of the central cutting flow path of the liquid chromatography analysis system. In this embodiment, the central cutting flow path 10 includes a central chromatographic column 11 and a central mobile phase delivery unit 12. The central chromatographic column 11 is used for preliminary separation of the sample, and the central mobile phase delivery unit 12 is used to provide the mobile phase to the central chromatographic column 11. The fraction output from the central chromatographic column 11 is selected for target cutting via the path switching component 20.

[0033] The central mobile phase delivery unit 12 is located upstream of the central chromatographic column 11 and is used to deliver a suitable mobile phase to the central chromatographic column 11 to regulate the separation behavior of the sample in the central chromatographic column 11. The control unit 40 is electrically connected to the central mobile phase delivery unit 12 to control its operating parameters, including the mobile phase flow rate and composition ratio, thereby achieving dynamic adjustment of the separation effect of the central chromatographic column 11. The control unit 40 can set the elution program for controlling the operation of the central mobile phase delivery unit 12 according to the sample characteristics and separation requirements, so that the central chromatographic column 11 achieves the best separation effect.

[0034] For example, a gradient elution method can be used to gradually change the polarity or intensity of the mobile phase, so that different components in the sample are eluted sequentially in the central chromatographic column 11, thereby achieving preliminary fractionation of various target fractions. During the gradient elution process, the control unit 40 can monitor the separation status of the central chromatographic column 11 in real time and dynamically adjust the elution program according to the detection signal to ensure that various target fractions are cut under optimal conditions.

[0035] The outlet end of the central chromatographic column 11 is connected to the path switching component 20, which can automatically switch the flow path at a preset time point or according to the detection signal to guide the target cut fraction to the corresponding secondary cut flow path 30.

[0036] The central chromatographic column 11 can be selected based on the characteristics of the sample, such as a reversed-phase column, a normal-phase column, or an ion-exchange column, to match the separation requirements of the target cut fraction in the sample.

[0037] The number of central chromatographic columns 11 is at least one. When there are multiple central chromatographic columns 11, they can be connected in parallel or in series to achieve simultaneous or gradient separation of different types of target cut fractions in the sample. The types of multiple central chromatographic columns 11 can be different. By combining central chromatographic columns 11 with different separation mechanisms, such as using a reversed-phase column and an ion-exchange column in series, efficient preliminary separation of multiple types of target cut fractions in complex samples can be achieved. In addition, multiple central chromatographic columns 11 can be configured with independent central mobile phase delivery units 12 to achieve differentiated control of mobile phase composition and flow rate, thereby optimizing the separation conditions of various target cut fractions in the central chromatographic column 11.

[0038] The central mobile phase delivery unit 12 may include at least one high-pressure pump and a corresponding solvent selection valve. The high-pressure pump provides a stable and precise mobile phase flow rate, while the solvent selection valve is used to prepare different types or proportions of mobile phase to meet the separation requirements of different samples. The flow rate delivered by the central mobile phase delivery unit 12 can be precisely adjusted within the range of 0.001 to 10 mL / min to meet the analytical needs of samples with different complexities.

[0039] Furthermore, the central cutting flow path 10 also includes an autosampler 13, which is disposed between the central chromatographic column 11 and the central mobile phase delivery unit 12. The autosampler 13 is disposed upstream of the central chromatographic column 11 and is used to automatically aspirate and inject the sample to be analyzed into the central chromatographic column 11. The central mobile phase delivery unit 12 is located upstream of the autosampler 13 and is used to provide the mobile phase to carry the sample into the central chromatographic column 11 for preliminary separation.

[0040] The autosampler 13 has high injection accuracy and can precisely control the injection volume, ensuring the consistency and accuracy of sample injection, thereby improving the repeatability and reliability of the separation results of the central chromatographic column 11.

[0041] The autosampler 13 also supports continuous injection of multiple batches of samples, improving sample processing efficiency and making it suitable for high-throughput analysis scenarios. Furthermore, the autosampler 13 has a wide injection volume adjustment range, typically adjustable precisely between 0.1 and 100 μL, to meet the analytical needs of samples with different concentrations. During injection, the autosampler 13 works in conjunction with the central mobile phase delivery unit 12 to ensure that the sample is thoroughly mixed with the mobile phase before entering the central chromatographic column 11, further improving separation efficiency. After sample injection, the control unit 40 can efficiently flush the autosampler 13 and flow path via an automatic cleaning program to avoid cross-contamination.

[0042] The control unit 40 controls the path switching component 20 based on a preset time window to import the initially segmented target cutting fraction into the corresponding secondary cutting flow path 30.

[0043] Under the precise timing control of the control unit 40, the path switching component 20 can achieve a millisecond-level switching response, thereby ensuring that the target cut fraction enters the corresponding secondary cutting flow path 30 at the optimal time.

[0044] The preset time window is based on the retention time and separation characteristics of the sample components in the central chromatographic column 11. By precisely controlling the cutting time window, the target fraction can be efficiently and accurately separated.

[0045] The path switching component 20 includes at least one switching valve for controlled introduction of the initially separated target cut fractions into different secondary cut flow paths 30.

[0046] The path switching component 20 is connected to the output end of the central chromatographic column 11. For example, the path switching component 20 includes a switching valve, which includes multiple flow path ports, wherein at least one flow path port is connected to the output end of the corresponding central chromatographic column 11. For example, the output ends of multiple central chromatographic columns 11 connected in parallel are respectively connected to the corresponding flow path ports; wherein at least two flow path ports are respectively connected to different secondary cutting flow paths 30. Under the control of the control unit 40, the switching valve switches the connection state between each flow path port to achieve directional splitting of different target cutting fractions.

[0047] See Figure 3 , Figure 3 yes Figure 1 The diagram shows the connection structure of the central cutting flow path and the path switching component of the liquid chromatography analysis system.

[0048] In this embodiment, the liquid chromatography analysis system 100 further includes a main path detector 14 and a waste liquid collector 15; the path switching component 20 includes a first switching valve 21 and a second switching valve 22 connected to each other. The first switching valve 21 is connected to the central cutting flow path 10, the main path detector 14 and the waste liquid collector 15, and the second switching valve is connected to each secondary cutting flow path; wherein, the first switching valve 21 is used to transport the target cutting fraction output from the central cutting flow path 10 to the second switching valve 22, and to transport the non-target cutting fraction output from the central cutting flow path 10 to the main detector 14 or the waste liquid collector 15; the second switching valve 22 distributes the received target cutting fraction to the corresponding secondary cutting flow path 30.

[0049] The first switching valve 21 can be used to transport the non-target cut fraction output from the central cut flow path 10 to the main path detector 14 for online detection, or to guide the waste liquid collector 15 for waste treatment, thereby achieving effective diversion and management of the non-target fraction.

[0050] The multiple output terminals of the second switching valve 22 are respectively connected to the input terminals of each secondary cutting flow path 30. The first switching valve 21 is also used to transport the target cutting fraction output from the central cutting flow path 10 to the input terminal of the second switching valve 22, and the second switching valve 22 accurately distributes various target cutting fractions to the corresponding secondary cutting flow paths 30 according to the control signal of the preset time window.

[0051] Through the coordinated operation of the first switching valve 21 and the second switching valve 22, the liquid chromatography analysis system 100 can achieve efficient and accurate target fraction capture and path switching during the analysis of complex samples, thereby improving the analysis speed and overall throughput of multiple target components in complex samples.

[0052] The first switching valve 21 is a six-way switching valve or a ten-way switching valve, and the second switching valve 22 is a four-way switching valve or a six-way switching valve.

[0053] For example, each secondary cutting flow path 30 can be equipped with an independent secondary infusion pump. The output end of the secondary infusion pump can be connected to a flow path port of the second switching valve 22. The second switching valve 22 controls the on / off state between the corresponding secondary infusion pump and the corresponding secondary cutting flow path 30, thereby realizing independent and precise pressure or flow rate control of the mobile phase in different secondary cutting flow paths 30, thereby optimizing the separation and transport conditions of the target fraction in each secondary cutting flow path 30.

[0054] For example, the first switching valve 21 is a six-way switching valve, whose six flow ports are respectively connected to the output end of the central chromatographic column 11, the output end of the central mobile phase delivery unit 12, the input end of the autosampler 13, the main path detector 14, the waste liquid collector 15, and the input end of the second switching valve 22. The first switching valve 21 can control the flow path from the central mobile phase delivery unit 12 to the autosampler 13, control the flow path between the central chromatographic column 11 and the main path detector 14 or the waste liquid collector 15, and control the flow path between the central chromatographic column 11 and the second switching valve 22, thereby achieving precise guidance and path selection of the mobile phase and the cut fraction in the central cutting flow path 10.

[0055] Alternatively, if the first switching valve 21 is a ten-way switching valve, then multiple high-pressure pumps in the central mobile phase delivery unit 12 can be connected to different flow path ports of the first switching valve 21 to achieve independent control and switching of different mobile phase components; or, multiple path detectors 14 or multiple waste liquid collectors 15 can be connected to different flow path ports of the first switching valve 21 to achieve flexible processing of different detection requirements or waste liquid classification.

[0056] See also Figure 3 and Figure 4 ,Figure 4 yes Figure 1 The diagram shows the connection structure of each secondary cutting flow path and path switching component of the liquid chromatography analysis system.

[0057] When the second switching valve 22 is a four-way switching valve, its four flow path ports are respectively connected to the output end of the first switching valve 21, the input ends of the two secondary cutting flow paths 30 and the waste liquid collector 34. The second switching valve 22 can guide the target fraction to the corresponding secondary cutting flow path 30 or guide the waste liquid to the waste liquid collector 15 by controlling the connection state between each flow path port.

[0058] When the second switching valve 22 is a six-way switching valve, the secondary infusion pumps equipped in each secondary cutting flow path 30 are respectively connected to different flow path ports of the second switching valve 22.

[0059] In this embodiment, the secondary cutting flow path 30 includes a secondary chromatographic column 31 and a secondary detector 32. The secondary chromatographic column 31 is used to further separate the allocated target cutting fraction, and the secondary detector 32 is used to detect the components separated by the secondary chromatographic column 31 and generate an analytical signal. The secondary chromatographic column 31 in each secondary cutting flow path 3 is of a different type.

[0060] For example, in one of the secondary cutting flow paths 30, the secondary chromatographic column 31 is a phenylhexyl chromatographic column, and the corresponding secondary detector 32 is an ultraviolet detector; in the other secondary cutting flow path 30, the secondary chromatographic column 31 is an amino chromatographic column, and the corresponding secondary detector 32 is a fluorescence detector, thereby achieving targeted separation and detection of target fractions with different chemical properties.

[0061] In each secondary cutting flow path 30, the types of secondary chromatographic columns 31 and secondary detectors 32 can be configured according to the chemical characteristics of the target fraction to be analyzed, so as to meet the analytical needs of compounds with different polarities, molecular weights or functional groups.

[0062] The secondary chromatographic column 31 can be flexibly selected according to the properties of the target analyte, such as using columns with different packing types or different pore sizes to achieve the best separation effect for a specific analyte. The secondary detector 32 can be one of an ultraviolet detector, a mass spectrometer, or a fluorescence detector.

[0063] The mobile phase of the secondary cutting flow path 30 can be the mobile phase provided by the central mobile phase delivery unit 12, or a secondary mobile phase delivery unit 32 can be set up independently to achieve a dedicated mobile phase supply to the secondary chromatographic column 31, further improving the separation effect and detection sensitivity.

[0064] In this embodiment, the secondary cutting flow path 30 further includes a secondary mobile phase delivery unit 33, which is used to provide a corresponding mobile phase to the secondary chromatographic column 31. The secondary mobile phase delivery unit 33 can be used to independently control the mobile phase composition, flow rate or gradient elution program.

[0065] By setting up an independent secondary mobile phase delivery unit 33, the separation conditions of the secondary chromatographic column 31 can be flexibly adjusted to meet the needs of fine separation of complex components.

[0066] The control unit 40 is electrically connected to the first switching valve 21, the second switching valve 22, the main path detector 14, the secondary detector 32, the central mobile phase delivery unit, the secondary mobile phase delivery unit, and the autosampler 13, and is used to coordinate the working timing and parameter settings of the various components of the system. Through a preset control program, the control unit 400 can achieve precise control of the central cutting flow path 10, the path switching component 20, and multiple parallel secondary cutting flow paths 30, so that different types of target cutting fractions are introduced into different secondary cutting flow paths 30 in parallel or alternately, thereby achieving efficient and high-sensitivity analysis of multiple target compounds in complex samples. The control unit 40 also collects the detection signals from the main path detector 14 and each secondary detector 32, and performs data integration and analysis to generate comprehensive analytical results.

[0067] Through the synergistic effect of the central cutting flow path 10, the path switching component 20, and multiple parallel secondary cutting flow paths 30, the liquid chromatography analysis system 100 avoids the waiting time associated with traditional tandem central cutting, significantly improving the analysis speed and overall throughput for multiple target cut components in complex samples. Combining the advantages of central cutting technology, the system effectively improves the separation degree and detection accuracy of target analytes in complex sample matrices through preliminary separation by the central column 11 and fine separation by the secondary columns 31. The liquid chromatography analysis system 100 can flexibly select whether to perform central cutting, the cutting time window, and which secondary cutting flow path 30 to direct the target cut fraction according to the complexity of the sample and the properties of the target analytes. The secondary columns 31 and mobile phase conditions of different secondary cutting flow paths 30 can be independently optimized to adapt to the separation requirements of various target analytes. Furthermore, the main path detector 14 can detect non-target cut fractions, and the secondary detectors 32 of each secondary cutting flow path 30 can detect different cut components, achieving comprehensive analysis of components with different properties in the sample.

[0068] The above descriptions are merely embodiments of this application and do not limit the patent scope of this application. Any equivalent structural or procedural transformations made based on the description and drawings of this application, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of this application. The technical solutions in the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of this application, not all of them. All other embodiments obtained by those skilled in the art based on the embodiments of this application without creative effort are within the protection scope of this application.

Claims

1. A liquid chromatography analysis system, characterized in that, The liquid chromatography analysis system includes: The central cutting flow path is configured to perform initial segmentation of the sample; At least two secondary cutting flow paths are provided, and each of the secondary cutting flow paths is connected in parallel. A path switching component connects the central cutting flow path and the at least two secondary cutting flow paths; The control unit, which is communicatively connected to the path switching component, is used to control the path switching component to introduce at least two types of target cut fractions separated by the central cutting flow path into different secondary cutting flow paths in a parallel or alternating manner.

2. The liquid chromatography analysis system according to claim 1, characterized in that, The control unit controls the path switching component to import the initially segmented target cut fraction into the corresponding secondary cut flow path based on a preset time window.

3. The liquid chromatography analysis system according to claim 1, characterized in that, The path switching component includes at least one switching valve, which is used to controllably guide each of the initially separated target cut fractions into different secondary cut flow paths.

4. The liquid chromatography analysis system according to claim 3, characterized in that, The liquid chromatography analysis system also includes a main path detector and a waste liquid collector; The path switching component includes a first switching valve and a second switching valve connected to each other. The first switching valve is connected to the central cutting flow path, the main path detector and the waste liquid collector, and the second switching valve is connected to each of the secondary cutting flow paths. The first switching valve is used to transport the target cut fraction output from the central cutting flow path to the second switching valve, and to transport the non-target cut fraction output from the central cutting flow path to the main detector or the waste liquid collector; the second switching valve then distributes the received target cut fraction to the corresponding secondary cutting flow path.

5. The liquid chromatography analysis system according to claim 4, characterized in that, The first switching valve is a six-way switching valve or a ten-way switching valve, and the second switching valve is a four-way switching valve or a six-way switching valve.

6. The liquid chromatography analysis system according to claim 1, characterized in that, The secondary cutting flow path includes a secondary chromatographic column and a secondary detector. The secondary chromatographic column is used to further separate the allocated target cutting fraction, and the secondary detector is used to detect the components separated by the secondary chromatographic column and generate an analytical signal. The secondary chromatographic columns in each of the secondary cutting flow paths are of different types.

7. The liquid chromatography analysis system according to claim 6, characterized in that, The secondary detector is one of an ultraviolet detector, a mass spectrometer, or a fluorescence detector.

8. The liquid chromatography analysis system according to claim 6, characterized in that, The secondary cutting flow path also includes a secondary mobile phase delivery unit, which is used to provide the corresponding mobile phase to the secondary chromatographic column and to independently control the mobile phase composition, flow rate, or gradient elution program.

9. The liquid chromatography analysis system according to claim 1, characterized in that, The central cutting flow path includes a central chromatographic column and a central mobile phase delivery unit. The central chromatographic column is used for preliminary separation of the sample, and the central mobile phase delivery unit is used to provide the mobile phase to the central chromatographic column. The fraction output from the central chromatographic column is selected for target cutting via the path switching component.

10. The liquid chromatography analysis system according to claim 9, characterized in that, The central cutting flow path also includes an autosampler, which is disposed between the central chromatographic column and the central mobile phase delivery unit. The autosampler is used to automatically aspirate and inject the sample to be analyzed into the central chromatographic column.

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