Method for improving salt resistance of common vetch by utilizing piperidine acid
By adding piperidine acid to arrowhead pea seeds, the problem of insufficient germination ability under salt stress was solved, and efficient germination of seeds in saline soil was achieved, which improved land use efficiency and reduced improvement costs.
Patent Information
- Application Number
- CN202511364613.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-09-23
- Publication Date
- 2025-12-16
AI Technical Summary
Arrowhead pea seeds have a weak germination ability under salt stress, which limits their application in saline soils and affects the effective use of land and the sustainability of crop production.
Piperidine acid was used to treat arrow pea seeds. By adding piperidinic acid to salt solutions of different concentrations, the salt tolerance of the seeds was improved. The specific steps included covering the seeds with sterile filter paper in petri dishes to check germination, and recording the seed germination and growth status.
It significantly improved the germination rate and shortened the germination time of arrowhead pea under salt stress, improved the utilization efficiency of saline soil, and reduced the improvement cost.
Smart Images
Figure CN121128556A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of crop production technology, and in particular to a method for improving the salt tolerance of arrowhead pea using piperidine acid. Background Technology
[0002] Salt-alkali stress is a serious environmental problem that significantly and adversely affects plant growth, development, and yield, thereby threatening the effective use of land and the sustainability of crop production. Of the world's 1.5 billion hectares of arable land, nearly 20%-30% suffer from varying degrees of soil salinization. Whether plants can germinate in saline soil is the first key issue they face, and the utilization and improvement of saline soil is of great significance.
[0003] To resist salt stress, plants undergo complex physiological changes, accumulating a large number of stress-response metabolites. These substances can alleviate salt stress and effectively improve the salt tolerance of plants. In production applications, screening key substances through metabolomics for exogenous application has significant application value. Previous salt tolerance experiments have found that piperidine acid accumulates significantly during plant salt stress responses and plays an important role, possessing the potential to improve plant salt tolerance. Furthermore, it has not been reported in the salt-tolerant germination of *Vaccaria buergeriana* seeds.
[0004] *Vicia sativa*, belonging to the genus *Vicia* in the family Fabaceae, is characterized by its short growth cycle and high forage yield, making it an important green manure crop that can improve soil fertility and restore the environment. While *Vicia sativa* plants exhibit some salt stress tolerance, their seed germination is significantly limited by salt stress concentration. This is one of the main reasons why many crops have poor survival ability under natural saline-alkali stress environments. This invention uses *Vicia sativa*, a plant with relatively weak salt tolerance and germination ability, as experimental material to test its seed salt tolerance and germination. The experimental results are expected to provide an effective approach to solving the problem of high-salt seed germination in plants, which is of significant research importance for restoring coastal saline-alkali land, increasing land area, and developing saline-alkali land agriculture. Summary of the Invention
[0005] The purpose of this invention is to address the shortcomings of existing technologies by proposing a method for improving the salt tolerance of arrowhead pea using piperidine acid. This method is simple and feasible, uses inexpensive raw materials, and has a rapid effect, effectively improving the germination rate of arrowhead pea under salt stress and shortening the germination time.
[0006] To achieve the above objectives, the present invention adopts the following technical solution:
[0007] A method for improving the salt tolerance of arrowhead pea using piperidine acid includes the following steps:
[0008] Step 1: Prepare two treatment solutions, one containing piperidine acid and the other without, using 156 mM NaCl and 200 mM NaCl solutions respectively;
[0009] Step 2: Select plump and high-quality arrowhead pea seeds, wash them with clean water, and cultivate them under suitable conditions;
[0010] Step 3: Cover the petri dish with sterile filter paper to check the germination. Use salt treatment as a control to compare the germination of arrow peas after applying different concentrations of piperidine acid. The culture conditions are the same as in Step 2.
[0011] Preferably, the salt treatment liquid in step one is divided into two types: one with piperidine acid applied and one without piperidine acid applied.
[0012] Preferably, in step one, a piperidine acid solution is prepared using NaCl solution, and the concentration of piperidine acid is 0.005-0.2 mM. Here, the preferred concentrations of piperidine acid are 0.005 mM, 0.01 mM, 0.05 mM, 0.1 mM and 0.2 mM, respectively.
[0013] Preferably, in step two, the growing conditions for arrowhead peas are: a day / night temperature cycle of 27°C / 25°C, 70% relative humidity, and a light / dark cycle of 16 hours of light / 8 hours of darkness; the treatment solution is changed daily.
[0014] Preferably, in step three, the detection of seed salt tolerance germination ability is carried out as follows: record the number of seeds germinating in each treatment group every day, observe the growth status, and take photos.
[0015] Compared with the prior art, the present invention has the following beneficial effects:
[0016] This invention applies piperidine acid to salt stress solution, which can effectively improve the germination rate of arrowhead pea seeds under salt stress and shorten the germination time, thereby improving the germination ability of arrowhead pea under salt stress, improving the effective utilization of saline soil, and reducing the cost of saline soil improvement. Attached Figure Description
[0017] Figure 1 This is a graph showing the effect of different concentrations of piperidine acid on the germination rate of arrowhead pea under 156 mM NaCl stress.
[0018] Figure 2 The phenotypic diagram shows the effect of different concentrations of piperidine acid on the germination of arrowhead pea under 156 mM NaCl stress according to the present invention.
[0019] Figure 3 This is a graph showing the effect of different concentrations of piperidine acid on the germination rate of arrowhead pea under 200 mM NaCl stress.
[0020] Figure 4 The phenotypic diagram shows the effect of different concentrations of piperidine acid on the germination of arrowhead pea under 200 mM NaCl stress according to the present invention.
[0021] In this context, 156S represents treatment with 156 mM NaCl, 0.005P represents the application of 0.005 mM piperidine acid, 0.01P represents the application of 0.01 mM piperidine acid, 0.05P represents the application of 0.05 mM piperidine acid, and 0.1P represents the application of 0.1 mM piperidine acid. 200S represents treatment with 200 mM NaCl, 0.01P represents the application of 0.01 mM piperidine acid, 0.1P represents the application of 0.1 mM piperidine acid, and 0.2P represents the application of 0.2 mM piperidine acid. Detailed Implementation
[0022] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings, so that those skilled in the art can better understand the advantages and features of the present invention, thereby making a clearer definition of the scope of protection of the present invention. The embodiments described in this invention are only some embodiments of the present invention, not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.
[0023] The seed material used in the examples in this specification is arrowhead pea. Piperidic acid was provided by Shanghai Maclean Biochemical Technology Co., Ltd., with a content >97%. NaCl is a conventional chemical reagent.
[0024] Example 1:
[0025] A method for improving the salt tolerance of arrowhead pea using piperidine acid includes the following steps:
[0026] Step 1: Select 50 plump and high-quality arrowhead pea seeds, wash them twice with clean water, and use them for experimental culture. Culture conditions: 27°C / 25°C day and night temperature cycle, 70% relative humidity, and 16 hours light / 8 hours dark light cycle; change the treatment solution daily.
[0027] The treatment solutions in step two are divided into two types: salt treatment and exogenous application of piperidine acid treatment solution. The salt treatment solution is a 156 mM NaCl solution, labeled as 156 S; the piperidine acid treatment solution is a piperidine acid solution prepared from the 156 mM NaCl solution, with piperidine acid concentrations of 0.005 mM, 0.01 mM, 0.05 mM and 0.1 mM, labeled as 156S+0.005P, 156S+0.01P, 156S+0.05P and 156S+0.1P.
[0028] Step 3: Check the germination and growth of seeds under different treatment solutions in Step 2. Record the number of germinated seeds, calculate the seed germination rate, and take photos every time the treatment solution is changed.
[0029] Effect:
[0030] Applying piperidine acid can significantly increase the germination rate of arrowhead pea under 156 mM salt stress. The germination rate of the 156S+0.1P group was 80%, which was 1.6 times that of the 156S group. Applying piperidine acid can also advance the germination of plants.
[0031] Example 2:
[0032] The salt treatment concentration was 200 mM NaCl solution, and the piperidine acid solution was prepared from 200 mM NaCl solution at concentrations of 0.01 mM, 0.1 mM, and 0.2 mM, labeled as 200 S, 200 S+0.01 P, 200 S+0.1 P, and 200 S+0.2 P, respectively. The rest was the same as in Example 1.
[0033] Effect:
[0034] Applying piperidine acid significantly increased the germination rate and shortened the germination time of arrowhead pea under 200 mM salt stress. The germination rate of the 0.2 mM piperidine acid treatment group was as high as 64%, which was 2.9 times that of the 200 mM group.
[0035] The above embodiments are combined with the appendix Figure 1 , 2 As shown in sections 3 and 4, treatment with 0.005–0.1 mM piperidinic acid can increase the germination rate of *Vaccaria buergeriana* under 156 mM NaCl stress by up to 1.6 times, and treatment with 0.01–0.2 mM piperidinic acid can increase the germination rate of *Vaccaria buergeriana* under 200 mM NaCl stress by up to 2.9 times. Both treatments significantly shorten the germination time of *Vaccaria buergeriana* under salt stress. No obvious abnormalities were observed after the addition of piperidinic acid compared with normally germinating seeds, indicating that this treatment method is safe and has no toxic effects on plants.
[0036] In summary, this invention can significantly improve the germination rate of seeds under salt stress. The treatment method of this invention is simple, has low raw material cost, and is fast-acting. It can be used to improve the germination of seeds under salt stress and has great potential for development and application in saline-alkali land.
[0037] The descriptions and practices disclosed in this invention are readily apparent and understandable to those skilled in the art, and various modifications and refinements can be made without departing from the principles of this invention. Therefore, any modifications or improvements made without departing from the spirit of this invention should also be considered within the scope of protection of this invention.
Claims
1. A method for improving the salt tolerance of arrowhead pea using piperidine acid, characterized in that, Includes the following steps: Step 1: Prepare two treatment solutions, one containing piperidine acid and the other without, using 156 mM NaCl and 200 mM NaCl solutions respectively; Step 2: Select plump and high-quality arrowhead pea seeds, wash them with clean water, and cultivate them under suitable conditions; Step 3: Cover the petri dish with sterile filter paper to check the germination. Use salt treatment as a control to compare the germination of arrow peas after applying different concentrations of piperidine acid. The culture conditions are the same as in Step 2.
2. The method for improving the salt tolerance of arrowhead pea using piperidine acid according to claim 1, characterized in that, In step one, the salt treatment liquid used is divided into two types: one with piperidine acid added and one without piperidine acid added.
3. The method for improving the salt tolerance of arrowhead pea using piperidine acid according to claim 1, characterized in that, In step one, piperidine acid solution is prepared using NaCl solution, and the concentration of piperidine acid is 0.005-0.2 mM.
4. The method for improving the salt tolerance of arrowhead pea using piperidine acid according to claim 1, characterized in that, In step two, the growing conditions for arrowhead peas are: a day-night temperature cycle of 27°C / 25°C, 70% relative humidity, and a light / dark cycle of 16 hours of light / 8 hours of darkness; the treatment solution is changed daily.
5. A method for improving the salt tolerance of arrowhead pea using piperidine acid according to claim 1, characterized in that, In step three, the detection of seed salt tolerance germination ability is carried out as follows: record the number of seeds germinating in each treatment group every day, observe the growth status, and take photos.