Propagation method of onychostoma macrolepis

Abstract

The invention discloses a breeding method of onychostoma macrolepis. The breeding method comprises the following steps: S1, selecting parent fishes; s2, the selected parent fishes are fed into a breeding pond to be cultured, and feed with crude protein larger than or equal to 35% is fed; s3, preparing oxytocin to inject the female parent fish and the male parent fish in the breeding season; s4, artificial insemination: mixing eggs with semen, standing for 1 minute, and adding clear water for activation; s5, fertilized eggs are disinfected and then put into an incubator to be hatched in running water; and S6, cultivating larvae, and feeding artemia nauplii and microparticle compound feed. Compared with the prior art, the breeding method has the advantages that operation and use are convenient, and large-scale production and use can be achieved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of breeding of Onychostoma lini, and particularly relates to a breeding method of Onychostoma lini. BACKGROUND

[0002] Onychostoma lini belongs to the Cyprinidae and is a kind of fish with high economic value and ecological value in China.

[0003] Onychostoma lini is a kind of freshwater fish with important economic value and ecological value, which is favored by consumers in the market, and has high edible and medicinal value due to its delicious and nutritious meat.

[0004] In recent years, with the continuous development and protection of China's fishery resources, the research and breeding of Cyprinidae have attracted widespread attention.

[0005] At present, the artificial breeding technology of Onychostoma lini has not formed a systematic, perfect and mature technical system. In the actual breeding process, there are problems such as uneven quality of parent fish, unstable spawning effect, low fertilized egg hatching rate, and low survival rate of larvae, which seriously restrict the development of the artificial breeding industry of Onychostoma lini. SUMMARY

[0006] The technical problem to be solved by the present application is to overcome the above technical defects, and to provide a breeding method of Onychostoma lini which is convenient to operate and use and can be used for large-scale production.

[0007] To solve the above technical problems, the technical scheme provided by the present application is as follows: a breeding method of Onychostoma lini, comprising the following steps:

[0008] S1: selecting parent fish;

[0009] S2: sending the selected parent fish to a breeding pond for culture and feeding with feed with crude protein ≥ 35%;

[0010] S3: injecting oxytocin to female parent fish and male parent fish during the breeding season;

[0011] S4: artificial insemination, mixing the eggs with the semen, standing for 1 minute, and adding clean water to activate;

[0012] S5: disinfecting the fertilized eggs and placing them in a hatcher for flowing water hatching;

[0013] S6: cultivating larvae and feeding with artemia nauplii and microparticle compound feed.

[0014] Preferably, the female fish selected in S1 is more than 3 years old and weighs ≥ 250 grams;

[0015] The male fish selected is more than 2 years old and weighs ≥ 150 grams.

[0016] Preferably, the abdomen of the female fish in S1 is swollen and soft, and light yellow free eggs flow out under light pressure;

[0017] The snout and anal fin of the male fish have white beads, and the dorsal fin and anal fin are bright and colorful.

[0018] Preferably, the feeding amount in S2 is 3% of the body weight of the fish, the water temperature in the breeding tank is 20-22℃, the dissolved oxygen is ≥5mg / L, and the pH value is 7.5-8.0.

[0019] Preferably, in S3, the oxytocin is mixed and injected at a ratio of 800-1000IU / kg of chorionic gonadotropin and 3mg / kg of diethylstilbestrol, and the injection dose of the male fish is halved.

[0020] Preferably, in S3, disposable dorsal fin base injection is used, and the effective time is 12 hours.

[0021] Preferably, in S5, the fertilized eggs are disinfected with povidone-iodine solution, and the hatching is carried out in a hatcher with water temperature of 21℃ and dissolved oxygen ≥6mg / L, and the hatching accumulated temperature is 84-105℃.

[0022] Preferably, in S5, the light intensity of the hatcher is 0.80-2.24W / m 2 , and the length of the hatched fry is 5-6mm, and the body color is light yellow.

[0023] Preferably, in S6, the fry is fed 8-9 days after membrane rupture, wherein the crude protein of the microparticle compound feed is ≥50%, and the particle size is ≤0.5mm.

[0024] Clostridium butyricum and multi-vitamin micro-ecological preparation are also added in the bait.

[0025] Compared with the prior art, the present application has the following advantages:

[0026] By accurately selecting the parent fish, the reproductive performance and health status of the parent fish are ensured, and by adjusting the ratio and injection method of oxytocin, the oxytocin effect is improved, so that the parent fish can lay eggs and ejaculate sperm on time and smoothly, thereby improving the reproductive efficiency;

[0027] In the process of artificial insemination, the standing time and activation method are accurately controlled, the sperm-egg combination rate is improved, Clostridium butyricum and multi-vitamin micro-ecological preparation are added to regulate the intestinal flora of the fry and enhance its immunity, thereby promoting the growth and development of the fry and significantly improving the survival rate of the fry;

[0028] The breeding method of the present application is standardized and controllable, and can provide technical support for the large-scale artificial breeding of the multi-scale white carp. BRIEF DESCRIPTION OF DRAWINGS

[0029] Figure 1 It is a flow chart of a breeding method of multi-scale white carp. DETAILED DESCRIPTION

[0030] The application will be further described in detail below with reference to the drawings.

[0031] With reference to the drawings, Figure 1 A breeding method of multi-scale white carp, comprising the following steps: S1: selecting parent fish; S2: sending the selected parent fish into a breeding pond for culture, and feeding the parent fish with feed with crude protein ≥ 35%; S3: injecting oxytocin to female parent fish and male parent fish in the breeding season; S4: artificial insemination, mixing eggs with semen, and then standing for 1 minute and adding clean water to activate; S5: disinfecting the fertilized eggs and then placing them into a flow water incubator for incubation; and S6: cultivating fry, and feeding the fry with artemia nauplii and microparticle compound feed.

[0032] In S1, the female fish selected are more than 3 years old and weigh ≥ 250 grams, and the male fish selected are more than 2 years old and weigh ≥ 150 grams; in S1, the abdomen of the female fish is swollen and soft, and light yellow free eggs flow out when being pressed; the snout and anal fin of the male fish are provided with white beads, and the dorsal fin and anal fin are bright and colorful.

[0033] In S2, the feeding amount is 3% of the weight of the fish, the water temperature of the breeding pond is 20-22℃, the dissolved oxygen is ≥ 5 mg / L, and the pH value is 7.5-8.0; in S3, the oxytocin is mixed and injected at a ratio of chorionic gonadotropin 800-1000 IU / kg and diethylstilbestrol 3 mg / kg, wherein the injection dose of the male fish is halved, and one-time dorsal fin base injection is used, and the effective time is 12 hours.

[0034] In S5, the fertilized eggs are disinfected with povidone-iodine solution, and the flow water incubation is carried out in an incubator with water temperature 21℃ and dissolved oxygen ≥ 6 mg / L, the incubation accumulated temperature is 84-105℃, the light intensity of the incubator is 0.80-2.24 W / m 2 , and the full length of the hatched fry is 5-6 mm, and the body color is light yellow.

[0035] In S6, the fry is fed 8-9 days after hatching, wherein the crude protein of the microparticle compound feed is ≥ 50%, and the particle size is ≤ 0.5 mm.

[0036] It also includes adding clostridium butyricum and multi-vitamin micro-ecological preparation in the bait.

[0037] In the specific implementation of the present application, before the breeding season, the population of Pseudobagrus vachelli is screened. According to the standard that the female fish is more than 3 years old, the body weight is greater than or equal to 250 grams, the abdomen is swollen and soft, and the light yellow free eggs flow out when being pressed, the male fish is more than 2 years old, the body weight is greater than or equal to 150 grams, the snout and the anal fin have white beads, and the dorsal fin and the anal fin are bright and colorful, the healthy parent fish is selected, and the selected parent fish is put into the breeding pond. The area of the breeding pond is reasonably determined according to the number of parent fish. The feed with the crude protein content of greater than or equal to 35% is fed to the fish at a rate of 3% of the body weight of the fish. At the same time, the water temperature, dissolved oxygen and pH value of the breeding pond are monitored in real time by using water quality monitoring equipment. The water temperature is controlled at 20-22℃, the dissolved oxygen is greater than or equal to 5mg / L, and the pH value is 7.5-8.0 by using heating or cooling equipment, oxygenation equipment and adding acid-base regulator.

[0038] In the breeding season, the oxytocin is configured according to the body weight and gender of the parent fish. The chorionic gonadotropin 800-1000IU / kg and diethylstilbestrol 3mg / kg are uniformly mixed to prepare an oxytocin solution. The disposable syringe is used to inject the parent fish at the base of the dorsal fin. The female fish is injected according to the calculated dose, and the male fish is injected at half the dose. After injection, the parent fish is placed in a quiet environment, and the effect time is 12 hours;

[0039] After the effect time ends, the behavior of the female parent fish is closely observed. When the female fish shows signs of spawning, it is quickly fished out, the fish body is wiped dry with a dry towel, the abdomen is lightly pressed, and the eggs are squeezed into a clean container. At the same time, the male fish is fished out, the sperm is squeezed out in the same way, the sperm is quickly poured into the container containing the eggs, the eggs and the sperm are fully mixed by gently stirring with a feather, then the mixture is left for 1 minute, and finally an appropriate amount of clean water is added to activate it.

[0040] The activated fertilized eggs are disinfected with povidone-iodine solution for 5-10 minutes. After disinfection, the fertilized eggs are evenly spread on the hatching net in the incubator. The water temperature in the incubator is maintained at 21℃, and the dissolved oxygen is greater than or equal to 6mg / L in a flowing water environment. The incubation accumulated temperature is controlled at 84-105℃ by adjusting the water flow rate and using heating equipment.

[0041] At the same time, the light intensity of the incubator is adjusted to 0.80-2.24W / m 2 After the larvae break the membrane, they are not fed for the first 7 days to allow them to absorb their yolk sacs. From the 8th to the 9th day after breaking the membrane, they are fed 3-4 times a day with Artemia nauplii and microparticle compound feed. When feeding the microparticle compound feed, Clostridium butyricum and multi-vitamin micro-ecological preparation are added to the feed at a certain ratio, stirred evenly and then fed. The breeding pond is regularly cleaned and watered to keep the water clean and provide a good environment for the growth of the larvae.

[0042] The contents not described in detail in the specification belong to the prior art known to those skilled in the art.

[0043] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this invention, "a plurality of" means two or more, unless otherwise explicitly specified.

[0044] The present invention and its embodiments have been described above. This description is not restrictive, and the accompanying drawings are only one embodiment of the present invention; the actual structure is not limited thereto. In conclusion, if those skilled in the art are inspired by this description and design similar structures and embodiments without departing from the spirit of the invention, such designs should fall within the protection scope of the present invention.

Claims

1. A method for breeding the multiscaled white carp, characterized in that: Includes the following steps: S1: Select parent fish; S2: Selected parent fish are transferred to a rearing pond for culture and fed with feed containing ≥35% crude protein; S3: During the breeding season, prepare oxytocin and inject it into both female and male broodstock. S4: Artificial insemination, mix the eggs and semen, let stand for 1 minute, then add water to activate; S5: After disinfecting the fertilized eggs, place them in an incubator for running water incubation; S6: Raise fry and feed them Artemia nauplii and microparticle feed.

2. The method for breeding the multiscaled white carp according to claim 1, characterized in that: In S1, female fish are selected that are 3 years or older and weigh ≥250 grams. Male fish should be 2 years or older and weigh ≥150 grams.

3. The method for breeding the multiscaled white carp according to claim 2, characterized in that: In S1, the female fish has a swollen and soft abdomen, and when gently pressed, pale yellow free eggs flow out. The male has white tubercles on its snout and anal fin, and its dorsal and anal fins are brightly colored for breeding.

4. The method for breeding the multiscaled white carp according to claim 1, characterized in that: In S2, the feeding amount is 3% of the fish's body weight, the water temperature in the rearing pond is 20-22℃, dissolved oxygen is ≥5mg / L, and pH value is 7.5-8.

0.

5. The method for breeding the multiscaled white carp according to claim 1, characterized in that: The oxytocin ratio in S3 is 800-1000 IU / kg of human chorionic gonadotropin and 3 mg / kg of dioxin, which are mixed and injected into the fish, with the injection dose for male fish reduced by half.

6. The method for breeding the multiscaled white carp according to claim 5, characterized in that: The S3 method uses a single-use injection at the base of the dorsal fin, with an effect time of 12 hours.

7. The method for breeding the multiscaled white carp according to claim 1, characterized in that: The S5 fertilized eggs were disinfected with povidone-iodine solution and incubated in an incubator with running water at a water temperature of 21°C and dissolved oxygen ≥6mg / L, with an accumulated incubation temperature of 84~105°C.

8. The method for breeding the multiscaled white carp according to claim 7, characterized in that: The light intensity of the incubator in S5 is 0.80–2.24 W / m². 2 The hatched fish fry are 5-6 mm in length and have a pale yellow body color.

9. The method for breeding the multiscaled white carp according to claim 1, characterized in that: The S6 feed is given to juvenile fish 8-9 days after hatching, and the crude protein content of the microparticle compound feed is ≥50% and the particle size is ≤0.5mm. This also includes adding Clostridium butyricum and multi-dimensional microecological preparations to the feed.

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