Cryopreservation method of in-vitro skin

Abstract

The in-vitro skin cryopreservation method comprises the following steps that S1, a certain amount of UW liquid is added into a base, and the liquid level is flush with a supporting plate 2; s2, the positions of a plurality of first clamping mechanisms are adjusted, and the in-vitro skin is placed on a supporting plate and makes contact with the first clamping mechanisms; s3, the positions of a plurality of second clamping mechanisms are adjusted, a top cover is aligned with the connection mechanism and pressed down, and the second clamping mechanisms and the first clamping mechanism clamp the edge of the in-vitro skin; s4, the adjusting mechanism is rotated, so that the first clamping mechanism and the second clamping mechanism slightly pull the in-vitro skin to extend towards the periphery; and S5, preserving the in-vitro skin in a sterile environment at the temperature of 4 DEG C or below.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of medical devices, in particular to a method for cryopreservation of ex vivo skin. BACKGROUND

[0002] Skin is the largest organ of the human body, and patients with difficult-to-heal wounds have various skin breakdown problems, which require local skin debridement and skin graft surgery. Many patients with difficult-to-heal wounds have a shortage of autologous skin, and fresh allogeneic skin is often difficult to obtain immediately, so ex vivo autologous skin is the first choice for skin grafting for such patients.

[0003] Currently, in clinical treatment, large hospitals store ex vivo skin removed during surgery in hospital refrigerators. This preservation method can easily cause the ex vivo skin to fold and atrophy during storage and transportation, affecting subsequent skin grafting operations, and therefore a method for cryopreservation of ex vivo skin is needed to solve the above problems. SUMMARY

[0004] The summary section of the present application is used to introduce the concept in a brief form, which will be described in detail in the specific embodiments section. The summary section of the present application is not intended to identify key or essential features of the claimed technical solution, nor is it intended to limit the scope of the claimed technical solution.

[0005] To solve the technical problems mentioned in the background section, some embodiments of the present application provide a method for cryopreservation of ex vivo skin, comprising the following steps: S1: adding a certain amount of UW solution to the base, the liquid level being flush with the support plate 2; S2: adjusting the position of the plurality of first clamping mechanisms, placing the ex vivo skin on the support plate in contact with the first clamping mechanisms; S3: adjusting the position of the plurality of second clamping mechanisms, aligning the top cover with the docking mechanism and pressing down, the second clamping mechanisms and the first clamping mechanisms clamping the edges of the ex vivo skin; S4: rotating the adjusting mechanism to slightly stretch the ex vivo skin outward by the first clamping mechanisms and the second clamping mechanisms; S5: storing the ex vivo skin in a sterile environment below 4℃; The first clamping mechanism comprises a first sliding rod fixed on the base, a second sliding rod fixed on the base, a sliding seat slidingly arranged on the first sliding rod and the second sliding rod, a first clamping plate fixed on the sliding seat, and a plurality of first protrusions fixed on the first clamping plate; The second clamping mechanism comprises a first connecting rod, a first adjusting ring rotatably arranged on the first connecting rod, a second connecting rod slidably arranged in the first connecting rod and engaged with the first adjusting ring, a first sliding groove fixedly arranged on the second connecting rod, a sliding block slidably arranged on the first sliding groove, an extension rod rotatably arranged on the sliding block, a second clamping plate fixedly arranged on one end of the extension rod, and a plurality of second protrusions fixedly arranged on the second clamping plate.

[0006] Specifically, the adapter mechanism comprises a first protrusion fixedly arranged on the base, a first sealing ring fixedly arranged on the first protrusion, a plurality of second protrusions fixedly arranged on the top cover, a second sealing ring fixedly arranged on the top cover, a plurality of buckle assemblies arranged on the base and the second protrusions, a plurality of pressing assemblies arranged on the base and the top cover, and a traction assembly arranged on the sliding seat and the second connecting rod.

[0007] Specifically, the buckle assembly comprises a second sliding groove fixedly arranged on the base, a buckle slidably arranged in the second sliding groove, a plurality of first springs having one end fixedly arranged on the buckle and the other end fixedly arranged on the base, and a plurality of clamping grooves fixedly arranged on the second protrusions and abutting against the buckle.

[0008] Specifically, the pressing assembly comprises a third sliding groove fixedly arranged on the base, a first sliding plate slidably arranged in the third sliding groove and fixedly connected with the support plate, a second spring having one end fixedly arranged on the support plate and the other end fixedly arranged on the base, and a first adapter fork fixedly arranged on the top cover and capable of contacting the first sliding plate.

[0009] Specifically, the traction assembly comprises a traction column fixedly arranged on the sliding seat and a second adapter fork rotatably arranged on one end of the second connecting rod and capable of contacting the traction column.

[0010] Specifically, the adjusting mechanism comprises an adjusting column rotatably arranged on the top cover, a plurality of grooves fixedly arranged on the adjusting column, and a second adjusting ring sleeved on the adjusting column and engaged with the adjusting column; the other end of the first connecting rod is rotatably arranged on the second adjusting ring.

[0011] The present application has the following advantages: (1) The height difference between the first clamping plate and the first protrusion can make the UW liquid infiltrate the isolated skin, so that the clamped part can also contact the UW liquid, thereby preventing the isolated skin from atrophy and necrosis.

[0012] (2) After the top cover is buckled, the first adapter fork pushes the first sliding plate to move downward, thereby making the support plate move downward and the second spring be compressed; the UW liquid level being flush with the support plate can prevent the isolated skin from moving due to liquid buoyancy when the top cover is not buckled, thereby causing the first clamping plate and the second clamping plate to fail to clamp the isolated skin; after the top cover is buckled, the support plate moves downward, so that the part of the isolated skin originally in contact with the support plate can also fully contact the UW liquid, thereby preventing partial necrosis of the isolated skin.

[0013] (3) After the top cover is buckled, the second connecting fork is in contact with the traction column, the first clamping plate and the second clamping plate clamp the excised skin, in order to prevent the excised skin from shrinking, the second adjusting column can be rotated, because the second connecting fork is limited by the traction column, the second adjusting ring cannot be rotated, so the adjusting column can drive the second adjusting ring to move downward, thereby making each second connecting fork push the sliding seat to move outward, thereby driving the first clamping plate and the second clamping plate to pull the excised skin to move outward, a small amount of tension is applied, avoiding the excised skin from shrinking.

[0014] (4) There is no cleaning dead angle between each part, the first connecting rod and the second connecting rod can be completely disassembled, most parts are in sliding contact or can be disassembled, and it is not easy to leave UW liquid, so it is convenient to clean and reuse. BRIEF DESCRIPTION OF DRAWINGS

[0015] The accompanying drawings, which form a part of this application, are included to provide a further understanding of the application and are incorporated in and constitute a part of this application. The drawings illustrate embodiments of the present application and, together with the description, serve to explain the principles of the present application.

[0016] In addition, throughout the drawings, same or similar reference numerals are used to represent same or similar elements. It should be understood that the drawings are schematic, and the elements and elements are not necessarily drawn according to the proportion.

[0017] In the drawings: Figure 1 is a structural schematic diagram of the present application; Figure 2 is a top view of the present application; Figure 3 is Figure 2 is a line section view of A-A in the figure; Figure 4 is Figure 3 is a local enlarged view at B in the figure; Figure 5 is Figure 3 is a local enlarged view at C in the figure; Figure 6 is Figure 3 is a local enlarged view at D in the figure; Figure 7 is Figure 2 is a line section view of E-E in the figure. DETAILED DESCRIPTION

[0018] Embodiments of the present disclosure will be described in more detail below with reference to the accompanying drawings. Although some embodiments of the present disclosure are shown in the drawings, it should be understood that the present disclosure can be implemented in various forms and should not be construed as being limited to the embodiments set forth herein. Rather, these embodiments are provided so that the present disclosure will be more thoroughly and completely understood. It should be understood that the drawings and embodiments of the present disclosure are only for illustrative purposes and are not intended to limit the scope of protection of the present disclosure.

[0019] In addition, it should be further noted that only parts related to the present application are shown in the drawings for ease of description. The embodiments in the present disclosure and the features in the embodiments can be combined with each other without conflict.

[0020] It should be noted that the concepts of "first", "second", etc. mentioned in the present disclosure are only used to distinguish different devices, modules or units, and are not used to limit the order or interdependence of the functions performed by these devices, modules or units.

[0021] It should be noted that the adjectives "one" and "multiple" mentioned in the present disclosure are illustrative and not limiting, and those skilled in the art should understand that unless otherwise explicitly stated in the context, it should be understood as "one or more".

[0022] The present disclosure will be described in detail below with reference to the accompanying drawings and in conjunction with the embodiments.

[0023] Referring to Figures 1-7 The method for cryopreservation of ex vivo skin according to the present application comprises the following steps: S1: A certain amount of UW solution is added to the base 1, and the liquid level is flush with the support plate 2; S2: Adjust the position of the plurality of first clamping mechanisms 3, and place the ex vivo skin on the support plate in contact with the first clamping mechanisms; S3: Adjust the position of the plurality of second clamping mechanisms 4, and press the top cover 7 against the docking mechanism 5, and the second clamping mechanisms and the first clamping mechanisms clamp the edges of the ex vivo skin; S4: Rotate the adjusting mechanism 6 to slightly stretch the ex vivo skin around the first clamping mechanisms and the second clamping mechanisms; S5: Store the ex vivo skin in a sterile environment below 4°C; The first clamping mechanism comprises a first sliding rod 31 fixed on the base, a second sliding rod 32 fixed on the base, a sliding seat 33 sliding on the first sliding rod and the second sliding rod, a first clamping plate 34 fixed on the sliding seat, and a plurality of first protrusions 35 fixed on the first clamping plate; the first sliding rod is provided with a scale, so that the moving distance of the sliding seat can be observed; the first protrusions on the first clamping plate are in contact with the isolated skin and cooperate with the second protrusions on the second clamping plate to clamp the isolated skin; the height difference between the first clamping plate and the first protrusions can enable the UW liquid to infiltrate the isolated skin, so that the clamped part can also be in contact with the UW liquid, thereby preventing the isolated skin from atrophy and necrosis; the UW liquid is a prior art; The second clamping mechanism comprises a first connecting rod 41, a first adjusting ring 42 rotatably arranged on the first connecting rod, a second connecting rod 43 slidingly arranged in the first connecting rod and engaged with the first adjusting ring, a first sliding groove 44 fixed on the second connecting rod, a sliding block 45 slidingly arranged on the first sliding groove, an extension rod 46 rotatably arranged on the sliding block, a second clamping plate 47 fixed on one end of the extension rod, and a plurality of second protrusions 48 fixed on the second clamping plate; the first connecting rod is provided with a protrusion, and the second connecting rod is provided with a groove matched with the protrusion for limiting the rotation of the second connecting rod; by rotating the first adjusting ring, the length of the second connecting rod extending out can be adjusted, which cooperates with the sliding seat to adapt to isolated skin of different sizes; the surface of the second connecting rod is provided with a thread engaged with the first adjusting ring; the surface of the second connecting rod is provided with a scale for conveniently observing the length of the extension.

[0024] Specifically, the connection mechanism comprises a first boss 51 fixed on the base, a first sealing ring 52 fixed on the first boss, a plurality of second bosses 53 fixed on the top cover, a second sealing ring 54 fixed on the top cover, a plurality of buckle assemblies 55 arranged on the base and the second bosses, a plurality of pressing assemblies 56 arranged on the base and the top cover, and a traction assembly 57 arranged on the sliding seat and the second connecting rod; the bottom of the second boss is provided with an inclined surface for facilitating insertion into the buckle assembly; the side surface of the second boss is provided with a groove.

[0025] Specifically, the buckle assembly comprises a second sliding groove 551 fixed on the base, a buckle 552 slidingly arranged on the second sliding groove, a plurality of first springs 553 fixed on one end of the buckle and fixed on the other end of the base, and a plurality of clamping grooves 554 fixed on the second boss and matched with the buckle; the top of the buckle is provided with an inclined surface for facilitating insertion of the second boss; the side surface of the buckle is provided with a plurality of protrusions which can be engaged with the groove on the side surface of the second boss to fix the top cover.

[0026] Specifically, the pressing assembly includes a third sliding groove 561 fixed on the base, a first sliding plate 562 slidingly arranged in the third sliding groove and fixedly connected with the support plate, a second spring 563 having one end fixed on the support plate and the other end fixed on the base, and a first connecting fork 564 fixed on the top cover and capable of contacting the first sliding plate. In the initial state, the first sliding plate is located at the top end of the third sliding groove, and the support plate is slightly higher than the first clamping plate. After the top cover is buckled, the first connecting fork pushes the first sliding plate to move downward, thereby moving the support plate downward, and the second spring is compressed. The UW liquid level is flush with the support plate, which can prevent the excised skin from moving due to liquid buoyancy when the top cover is not buckled, thereby causing the first clamping plate and the second clamping plate to fail to clamp the excised skin. After the top cover is buckled, the support plate moves downward, so that the part of the excised skin originally in contact with the support plate can also fully contact the UW liquid, preventing partial necrosis of the excised skin.

[0027] Specifically, the traction assembly includes a traction column 572 fixed on the sliding seat, and a second connecting fork 573 rotatably arranged at one end of the second connecting rod and capable of contacting the traction column.

[0028] Specifically, the adjusting mechanism includes an adjusting column 61 rotatably arranged on the top cover, a plurality of grooves 62 fixed on the adjusting column, and a second adjusting ring 63 sleeved on the adjusting column and engaged with the adjusting column. The other end of the first connecting rod is rotatably arranged on the second adjusting ring. The lower section of the adjusting column is provided with a thread, and the second adjusting ring is engaged with the adjusting column. The first connecting rod is damped when rotating on the adjusting ring and will not rotate due to its own weight. After the top cover is buckled, the second connecting fork contacts the traction column, and the first clamping plate and the second clamping plate clamp the excised skin. In order to prevent the excised skin from shrinking, the second adjusting column can be rotated. Since the second connecting fork is limited by the traction column, the second adjusting ring cannot be rotated, so the adjusting column can move the second adjusting ring downward, thereby causing each second connecting fork to push the sliding seat to move outward, and thereby causing the first clamping plate and the second clamping plate to pull the excised skin to move outward, thereby exerting a small amount of tension to prevent the excised skin from shrinking.

[0029] The above description is only some of the preferred embodiments of the present disclosure and an explanation of the technical principles applied. Those skilled in the art should understand that the scope of the application involved in the embodiments of the present disclosure is not limited to the technical solutions formed by the specific combinations of the above technical features, and should also cover other technical solutions formed by any combination of the above technical features or equivalent features without departing from the above inventive concept. For example, the above features can be replaced with each other to form technical solutions with similar functions disclosed in the embodiments of the present disclosure (but not limited to).

Claims

1. A method for cryopreservation of ex vivo skin, characterized in that, Includes the following steps: S1: Add a certain amount of UW liquid to the base, and the liquid level should be flush with the support plate; S2: Adjust the position of the multiple first clamping mechanisms, place the excised skin on the support plate, and make it contact with the first clamping mechanism; S3: Adjust the position of multiple second clamping mechanisms, align the top cover with the connecting mechanism and press it down, the second clamping mechanism and the first clamping mechanism clamp the edge of the detached skin; S4: Rotation adjustment mechanism, which slightly pulls the detached skin outwards using the first clamping mechanism and the second clamping mechanism; S5: Store the excised skin in a sterile environment below 4°C; The first clamping mechanism includes a first slide rod fixedly mounted on the base, a second slide rod fixedly mounted on the base, a sliding seat slidably mounted on the first slide rod and the second slide rod, a first clamping plate fixedly mounted on the sliding seat, and a plurality of first protrusions fixedly mounted on the first clamping plate; The second clamping mechanism includes a first connecting rod, a first adjusting ring rotatably disposed on the first connecting rod, a second connecting rod slidably disposed within the first connecting rod and meshing with the first adjusting ring, a first sliding groove fixedly disposed on the second connecting rod, a slider slidably disposed on the first sliding groove, a telescopic rod rotatably disposed on the slider, a second clamping plate fixedly disposed at one end of the telescopic rod, and a plurality of second protrusions fixedly disposed on the second clamping plate.

2. The method for cryopreservation of ex vivo skin according to claim 1, characterized in that: The connecting mechanism includes a first boss fixed on the base, a first sealing ring fixed on the first boss, multiple second bosses fixed on the top cover, a second sealing ring fixed on the top cover, multiple buckle assemblies on the base and the second bosses, multiple pressing assemblies on the base and the top cover, and a traction assembly on the sliding seat and the second connecting rod.

3. The method for cryopreservation of ex vivo skin according to claim 2, characterized in that: The buckle assembly includes a second slide groove fixedly disposed on the base, a buckle slidably disposed on the second slide groove, a plurality of first springs with one end fixedly disposed on the buckle and the other end fixedly disposed on the base, and a plurality of buckle slots fixedly disposed on the second protrusion and fitting with the buckle.

4. The method for cryopreservation of ex vivo skin according to claim 1, characterized in that: The pressing assembly includes a third slide groove fixedly mounted on the base, a first slide plate slidably mounted in the third slide groove and fixedly connected to the support plate, a second spring fixed at one end to the support plate and at the other end to the base, and a first connecting fork fixedly mounted on the top cover and capable of contacting the first slide plate.

5. The method for cryopreservation of ex vivo skin according to claim 2, characterized in that: The traction assembly includes a traction column fixedly mounted on a sliding seat and a second connecting fork rotatably mounted at one end of the second connecting rod and capable of contacting the traction column.

6. A method for cryopreservation of ex vivo skin according to claim 2, characterized in that: The adjustment mechanism includes an adjustment column rotatably mounted on the top cover, multiple grooves fixedly mounted on the adjustment column, and a second adjustment ring sleeved on and meshing with the adjustment column; the other end of the first connecting rod is rotatably mounted on the second adjustment ring.

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