Multi-layer exosome cryopreservation device

Abstract

The utility model discloses a multi-layer type exosome cryopreservation device which comprises a tank type shell and a nozzle sleeve arranged at the top end of the tank type shell, a tank nozzle is fixed in the nozzle sleeve, an inner container is arranged in the center of the tank type shell, and the bottom end of the inner container is fixedly connected with the inner wall of the bottom end of the tank type shell through a plurality of heat insulation fixing supports. The multiple heat insulation fixing supports can enable the inner container to be fixed in the tank type shell in a suspended mode, the novel lifting and protection device is additionally arranged outside the tank type shell, the lifting and protection device can achieve the protection effect through the multiple arc-shaped metal protection plates surrounding the tank type shell, and during the transportation period of the multi-layer exosome cryopreservation device, the multiple arc-shaped metal protection plates surround the tank type shell. A reliable anti-collision barrier can be formed, the risk that the device is damaged due to collision is greatly reduced, the safety of the device is effectively protected, a heat insulation pad on the inner wall of the device can remarkably reduce interference of external temperature, a stable low-temperature environment in the device can be maintained, and the cryopreservation effect of exosomes is guaranteed.

Description

Technical Field

[0001] This invention belongs to the technical field of biological sample storage, specifically relating to a multilayer exosome cryopreservation device. Background Technology

[0002] Exosomes, as important mediators of intercellular communication, have shown great application potential in many biomedical fields such as disease diagnosis, treatment, and drug development. Multilayer exosome cryopreservation devices are crucial for the long-term stable preservation of exosomes, effectively maintaining their bioactivity and integrity, and ensuring their effectiveness in subsequent research and applications.

[0003] However, existing multilayer exosome cryopreservation devices face numerous problems in practical use. Particularly during transportation and handling, the outer shell of these devices lacks effective protection. Due to the complex and variable transportation environment, the devices are inevitably subject to impacts or compression from external objects. Under such circumstances, the outer shell is easily deformed and damaged. Once deformed, the outer shell not only compromises the overall structural integrity of the device but also severely affects its sealing performance. Decreased sealing performance allows external heat to infiltrate, disrupting the originally stable low-temperature environment inside the device, thus affecting the appropriate temperature conditions required for exosome cryopreservation, reducing the cryopreservation effect, and even potentially causing the exosome samples to deteriorate and become ineffective. Furthermore, current cryopreservation devices are insufficient in adapting to different placement scenarios and usage requirements. In different experimental environments or storage locations, the height of the device cannot be flexibly adjusted, making it difficult to ensure stable and convenient placement, causing numerous inconveniences in actual operation. Utility Model Content

[0004] The purpose of this invention is to provide a multi-layer exosome cryopreservation device to solve the problems mentioned in the background art, such as the lack of effective protection of the canister shell during transportation and handling, easy deformation due to impact or compression, affecting the sealing and heat preservation effect, and the inability to flexibly adjust the height of the device, making it difficult to adapt to different scenarios and affecting the stability of placement and ease of operation.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a multi-layer exosome cryopreservation device, comprising a sealing cap, a canister-type outer shell, and a nozzle sleeve disposed at the top of the canister-type outer shell. A canister spout is fixed inside the nozzle sleeve. An inner liner is disposed inside the center of the canister-type outer shell. The bottom end of the inner liner is fixedly connected to the inner wall of the bottom end of the canister-type outer shell by multiple heat-insulating fixing brackets. The multiple heat-insulating fixing brackets allow the inner liner to be suspended and fixed in the canister-type outer shell. A neck tube is connected between the top end of the opening of the inner liner and the bottom end of the canister spout. A vacuum valve is also disposed on the upper side of the center of the right end of the canister-type outer shell, and the vacuum valve is connected to the inside of the canister-type outer shell. A lifting and protective device is disposed on the outside of the canister-type outer shell.

[0006] Preferably, the lifting and protective device includes an arc-shaped metal protective plate, a heat insulation pad, a vertical metal bracket, and a metal fixing ring. The outer circular walls at both ends of the tank-type shell are fitted with metal fixing rings. Four vertical metal brackets are equidistantly connected to the annular outer walls of the two metal fixing rings. An arc-shaped metal protective plate is connected between each of the four vertical metal brackets. The multiple arc-shaped metal protective plates and the four vertical metal brackets form a circular protective structure on the outside of the tank-type shell. The inner walls of the multiple arc-shaped metal protective plates are fixed with heat insulation pads.

[0007] Preferably, the outer walls of the four vertical metal supports are all arc-shaped, and the arc-shaped outer wall of the arc-shaped metal protective plate is flush with the arc-shaped outer wall of the vertical metal support. The arc-shaped metal protective plate and the vertical metal support are fixedly connected by welding. The arc-shaped metal protective plate, the vertical metal support and the metal fixing ring are all made of aluminum alloy.

[0008] Preferably, the lifting and protective device further includes a mounting base, an adjusting nut, and a lifting screw. Each of the four vertical metal supports has a lifting screw hole inside, with the opening of the lifting screw hole facing downwards. A lifting screw is threaded into the lifting screw hole, and an adjusting nut is sleeved and fixed to the bottom end of the lifting screw. The mounting base is welded to the bottom end of the lifting screw. The lifting screw can move up and down in the lifting screw hole by rotating clockwise and counterclockwise through the action of the thread.

[0009] Preferably, the outer walls of the can-shaped shell are welded with handling handles on the sloping outer walls at both ends of the top, the inner wall of the can-shaped shell is provided with an insulating inner pad, and the insulating inner pad is provided with multiple layers, and the outer wall of the inner liner is also provided with an absorbent.

[0010] Preferably, the inner walls of both the left and right ends of the nozzle are provided with rod fixing grooves, the top of the rod fixing grooves are open, and a sealing plug is provided at the center of the inner wall of the top of the sealing cap. The sealing plug can be completely inserted into the nozzle, and the sealing plug and the nozzle are interference fit.

[0011] Preferably, the inner liner is provided with a multi-layer cryopreservation rack, which is composed of a cryopreservation tray, an anti-detachment ring, an integrated bracket, a fixed top plate, a connecting rod, and a horizontal hanger. A connecting rod is welded to the center of the bottom end of the horizontal hanger. The left and right ends of the horizontal hanger can be inserted into two hanger fixing slots from top to bottom, respectively. The bottom end of the connecting rod passes through the neck tube and extends into the inner liner. A fixed top plate is fixed to the bottom end of the connecting rod, and the fixed top plate is located on the upper side of the center inside the inner liner.

[0012] Preferably, integrated brackets are welded to the bottom of both the left and right ends of the fixed top plate, and multiple cryopreservation trays are equidistantly connected between the two integrated brackets. The multiple cryopreservation trays are arranged equidistantly from top to bottom, and anti-detachment retaining rings are fixed to the outer wall of the top of each of the multiple cryopreservation trays.

[0013] Preferably, the diameter of the fixed top plate is smaller than the diameter of the neck tube and the nozzle. After the horizontal rod is horizontally fixed inside the rod fixing groove, multiple frozen storage trays are vertically suspended in the inner liner. After the horizontal rod is gripped, the multiple frozen storage trays can be lifted upwards through the connecting rod and the fixed top plate.

[0014] Compared with the prior art, this utility model provides a multi-layer exosome cryopreservation device, which has the following beneficial effects:

[0015] This invention incorporates a novel lifting and protective device on the exterior of the canister shell. This device, consisting of multiple arc-shaped metal protective plates surrounding the canister shell, provides protection and forms a reliable anti-collision barrier during transport of the multi-layer exosome cryopreservation device. This significantly reduces the risk of damage from collisions and effectively protects the device. The heat-insulating pad on the inner wall significantly reduces external temperature interference, helping to maintain a stable low-temperature environment inside the device and ensuring the cryopreservation effect of exosomes. Furthermore, the arc-shaped metal protective plates, vertical metal brackets, and metal fixing rings made of aluminum alloy combine lightweight and high strength, reducing the overall weight of the device while ensuring structural stability. Moreover, by rotating the adjusting nut, the lifting screw can be moved up and down in the lifting screw hole, allowing for flexible adjustment of the device height to better adapt to different placement scenarios and usage needs. This significantly improves the convenience and stability of the device, comprehensively enhancing the practicality and protective performance of the multi-layer exosome cryopreservation device. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the external three-dimensional structure of a multi-layer exosome cryopreservation device according to the present invention.

[0017] Figure 2 This is a cross-sectional three-dimensional structural diagram of a multi-layer exosome cryopreservation device according to the present invention.

[0018] Figure 3 This is a schematic diagram of the three-dimensional structure of the multi-layer cryopreservation rack of this utility model.

[0019] Figure 4 This is a three-dimensional structural diagram of the lifting and protective device of this utility model.

[0020] Figure 5 This is a partial disassembly diagram of the lifting and protective device of this utility model.

[0021] In the diagram: 1. Lifting and protective device; 2. Tank shell; 3. Spout; 4. Can spout; 5. Sealing cap; 6. Vacuum valve; 7. Insulated fixing bracket; 8. Inner liner; 9. Multi-layer cryogenic rack; 10. Insulating inner pad; 11. Neck tube; 12. Hanger fixing groove; 13. Sealing plug; 14. Cryogenic placement tray; 15. Anti-detachment retaining ring; 16. Integrated bracket; 17. Fixed top plate; 18. Connecting rod; 19. Horizontal hanger; 20. Placement base; 21. Adjusting nut; 22. Lifting screw; 23. Arc-shaped metal protective plate; 24. Insulating pad; 25. Vertical metal bracket; 26. Metal fixing ring. Detailed Implementation

[0022] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0023] This utility model provides, for example Figure 1-5 The multi-layer exosome cryopreservation device shown includes a sealing cap 5, a canister shell 2, and a nozzle 3 located at the top of the canister shell 2. A nozzle 4 is fixed inside the nozzle 3. An inner liner 8 is located inside the center of the canister shell 2. The bottom end of the inner liner 8 is fixedly connected to the inner wall of the bottom end of the canister shell 2 by multiple heat-insulating fixing brackets 7. These brackets 7 allow the inner liner 8 to be suspended and fixed within the canister shell 2. The canister shell 2 serves as an integral frame, with the inner liner 8 suspended and fixed within it by the multiple heat-insulating fixing brackets 7. These brackets ensure the stability of the inner liner 8 and effectively prevent heat transfer between the canister shell 2 and the inner liner 8, reducing the impact of external temperature on the cryopreservation environment inside the inner liner 8. A neck tube 11 connects the top opening of the inner liner 8 to the bottom end of the nozzle 4. A true neck tube is also provided on the upper side of the center of the right end of the canister shell 2. Vacuum valve 6 is connected to the interior of the canister shell 2. Vacuum valve 6 can create a vacuum inside the device. Combined with multi-layered heat-insulating pads 10, this greatly reduces heat conduction, further maintains a low-temperature stable cryopreservation environment inside the inner liner 8, reduces energy loss, and ensures that exosomes are preserved at a suitable temperature. The outer walls of the left and right ends of the top of the canister shell 2 are welded with handling handles. The handling handles facilitate the movement of the device by the operator, ensuring the convenience and safety of operation when transferring between different locations. The inner wall of the canister shell 2 is provided with heat-insulating pads 10, and the heat-insulating pads 10 are multi-layered. The outer wall of the inner liner 8 is also provided with an adsorbent. The adsorbent can adsorb moisture, impurities, and other substances that may be present in the inner liner 8, which are detrimental to the preservation of exosomes, improve the purity of the cryopreservation environment, extend the shelf life of exosomes, and ensure their quality.

[0024] like Figure 1 , Figure 2 and Figure 3 As shown, the inner walls of both ends of the nozzle 4 are provided with rod fixing grooves 12, and the top of the rod fixing grooves 12 is open. A sealing plug 13 is provided at the center of the inner wall of the top of the sealing cap 5. The sealing plug 13 can be completely inserted into the nozzle 4, and the sealing plug 13 and the nozzle 4 are interference fit. After the sealing plug 13 is completely inserted into the nozzle 4, it can effectively prevent external air, moisture and dust from entering the device, maintain the clean environment inside the inner liner 8, and avoid contamination of exosomes. The inner liner 8 is provided with multiple layers of cryopreservation racks 9, which are used for cryopreservation. The plate 14, anti-detachment retaining ring 15, integrated bracket 16, fixed top plate 17, connecting rod 18, and horizontal hanger 19 together constitute the structure. A connecting rod 18 is welded to the center of the bottom end of the horizontal hanger 19. The left and right ends of the horizontal hanger 19 can be inserted into two hanger fixing slots 12 from top to bottom, respectively. The bottom end of the connecting rod 18 passes through the neck tube 11 and extends into the inner liner 8. A fixed top plate 17 is fixed to the bottom end of the connecting rod 18, and the fixed top plate 17 is located on the upper side of the center inside the inner liner 8. Integrated brackets 16 are welded to the bottom of both the left and right ends of the fixed top plate 17. Multiple cryogenic storage trays 14 are equidistantly connected between the support brackets 16. These trays 14 are arranged equidistantly from top to bottom. Each tray 14 has an anti-detachment retaining ring 15 fixed to its top outer wall. The diameter of the fixed top plate 17 is smaller than the diameter of the neck tube 11 and the can spout 4. After the horizontal lifting rod 19 is horizontally fixed inside the lifting rod fixing groove 12, the multiple cryogenic storage trays 14 are vertically suspended in the inner liner 8. When the horizontal lifting rod 19 is gripped, the multiple cryogenic storage trays 14 can be lifted upwards via the connecting rod 18 and the fixed top plate 17. This multi-layer cryogenic rack 9 design... The horizontal rod 19 is fixed by inserting both ends into the rod fixing groove 12 on the inner wall of the nozzle 4. The connecting rod 18 connects the horizontal rod 19 to the fixed top plate 17, so that the cryopreservation tray 14 is vertically suspended in the inner liner 8. Multiple cryopreservation trays 14 are arranged at equal intervals, which increases the cryopreservation space of exosomes and improves the storage efficiency of the device. The anti-detachment ring 15 can prevent the exosome sample container from accidentally slipping during the cryopreservation process, ensuring the stability and safety of cryopreservation. Holding the horizontal rod 19 can easily lift the entire cryopreservation tray 14 assembly, which is convenient for sample loading and unloading operations.

[0025] like Figure 1 , Figure 4 and Figure 5As shown, a lifting and protective device 1 is installed on the outside of the tank shell 2. The lifting and protective device 1 includes an arc-shaped metal protective plate 23, a heat insulation pad 24, vertical metal supports 25, and metal fixing rings 26. Metal fixing rings 26 are fitted and fixed to the outer circular walls at both the top and bottom ends of the tank shell 2. Four vertical metal supports 25 are equidistantly connected to the annular outer walls of two metal fixing rings 26. Arc-shaped metal protective plates 23 are connected between the four vertical metal supports 25. The multiple arc-shaped metal protective plates 23 and the four vertical metal supports 25 form a circular protective structure on the outside of the tank shell 2. The metal fixing rings 26 are fitted onto the circular outer walls at the top and bottom ends of the tank shell 2, providing a stable installation base for the entire lifting and protective device 1. The four vertical metal supports 25 are equidistantly connected to the two metal fixing rings 26. A frame structure is formed on the annular outer wall, with arc-shaped metal protective plates 23 connected between vertical metal supports 25, surrounding the outside of the tank shell 2 to form a complete circular protective layer. This structural design can buffer and block external collisions and impacts in all directions, protecting the tank shell 2 and its important internal components. Especially during the handling and transportation of the device, it effectively reduces the risk of damage to the device due to accidental collisions. The inner walls of multiple arc-shaped metal protective plates 23 are fixed with heat insulation pads 24. These heat insulation pads 24 can effectively prevent the entry of external heat and the loss of internal low temperature. By reducing heat transfer, a stable low temperature environment is maintained inside the tank shell 2, ensuring the appropriate temperature conditions required for exosome cryopreservation, further improving the cryopreservation effect and extending the storage period of exosomes.

[0026] like Figure 1 , Figure 4 and Figure 5As shown, the outer walls of the four vertical metal supports 25 are all curved, and the curved outer wall of the curved metal protective plate 23 is flush with the curved outer wall of the vertical metal supports 25. The curved metal protective plate 23 and the vertical metal supports 25 are fixedly connected by welding. The curved metal protective plate 23, the vertical metal supports 25 and the metal fixing ring 26 are all made of aluminum alloy. Aluminum alloy has the advantages of light weight, high strength and corrosion resistance. Light weight can reduce the weight of the entire device, making it easy to handle and operate. High strength can ensure that the protective device can maintain the structural integrity when subjected to external impact, effectively playing a protective role. Corrosion resistance can extend the service life of the device and reduce the risk of structural damage caused by corrosion. The lifting and protective device 1 also includes a mounting base 20, an adjusting nut 21 and a lifting screw 22. The interior of the four vertical metal supports 25 is... The device is equipped with a lifting screw hole with its opening facing downwards. A lifting screw 22 is threaded into the lifting screw hole, and an adjusting nut 21 is sleeved and fixed to the bottom end of the lifting screw 22. A mounting base 20 is welded to the bottom end of the lifting screw 22. The lifting screw 22 can move up and down in the lifting screw hole by rotating clockwise and counterclockwise through the action of the thread. When it is necessary to adjust the height of the device, the adjusting nut 21 is rotated clockwise and counterclockwise. Since the adjusting nut 21 is fixed to the bottom end of the lifting screw 22, the lifting screw 22 will move up and down along the lifting screw hole under the action of the thread when the adjusting nut 21 is rotated. The mounting base 20 welded to the bottom end of the lifting screw 22 will change its height as the lifting screw 22 moves, thereby realizing the adjustment of the height of the entire device. This design allows the cryopreservation device to adapt to different placement planes and usage scenarios, ensuring the stability and convenience of the device placement.

[0027] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A multi-layer exosome cryopreservation device, comprising a sealing cap (5), a canister shell (2), and a nozzle sleeve (3) disposed at the top of the canister shell (2), wherein a canister spout (4) is fixed inside the nozzle sleeve (3), and an inner liner (8) is disposed inside the center of the canister shell (2), wherein the bottom end of the inner liner (8) is fixedly connected to the inner wall of the bottom end of the canister shell (2) by a plurality of heat-insulating fixing brackets (7), wherein the plurality of heat-insulating fixing brackets (7) can suspend and fix the inner liner (8) in the canister shell (2), wherein a neck tube (11) is connected between the top end of the opening of the inner liner (8) and the bottom end of the canister spout (4), and a vacuum valve (6) is also disposed on the upper side of the center of the right end of the canister shell (2), and the vacuum valve (6) is connected to the inside of the canister shell (2), characterized in that: The outer shell (2) of the tank is provided with a lifting and protective device (1); The lifting and protective device (1) includes an arc-shaped metal protective plate (23), a heat insulation pad (24), a vertical metal bracket (25), and a metal fixing ring (26). The outer circular walls at both ends of the tank shell (2) are fitted with metal fixing rings (26). The outer rings of the two metal fixing rings (26) are equidistantly connected to four vertical metal brackets (25). The arc-shaped metal protective plate (23) is connected between the four vertical metal brackets (25). The multiple arc-shaped metal protective plates (23) and the four vertical metal brackets (25) form a circular protective structure on the outside of the tank shell (2). The inner walls of the multiple arc-shaped metal protective plates (23) are fixed with heat insulation pads (24).

2. The multilayer exosome cryopreservation device according to claim 1, characterized in that: The outer walls of the four vertical metal supports (25) are all arc-shaped, and the arc-shaped outer wall of the arc-shaped metal protective plate (23) is flush with the arc-shaped outer wall of the vertical metal support (25). The arc-shaped metal protective plate (23) and the vertical metal support (25) are fixedly connected by welding. The arc-shaped metal protective plate (23), the vertical metal support (25) and the metal fixing ring (26) are all made of aluminum alloy.

3. The multilayer exosome cryopreservation device according to claim 2, characterized in that: The lifting and protective device (1) also includes a mounting base (20), an adjusting nut (21), and a lifting screw (22). Each of the four vertical metal brackets (25) has a lifting screw hole inside, and the opening of the lifting screw hole faces downward. The lifting screw (22) is threaded into the lifting screw hole. The adjusting nut (21) is sleeved and fixed on the outside of the bottom end of the lifting screw (22). The mounting base (20) is welded to the bottom end of the lifting screw (22). The lifting screw (22) can move up and down in the lifting screw hole by rotating clockwise and counterclockwise through the action of the thread.

4. The multilayer exosome cryopreservation device according to claim 1, characterized in that: The outer walls of the can-type shell (2) are welded with handling handles on the sloping outer walls at the top left and right ends. The inner wall of the can-type shell (2) is provided with an insulating inner pad (10), and the insulating inner pad (10) is provided with multiple layers. The outer wall of the inner liner (8) is also provided with an absorbent.

5. The multilayer exosome cryopreservation device according to claim 1, characterized in that: The inner walls of both the left and right ends of the nozzle (4) are provided with rod fixing grooves (12). The top of the rod fixing groove (12) is open. A sealing plug (13) is provided at the center of the inner wall of the top of the sealing cover (5). The sealing plug (13) can be completely inserted into the nozzle (4), and the sealing plug (13) and the nozzle (4) are interference fit.

6. The multilayer exosome cryopreservation device according to claim 5, characterized in that: The inner liner (8) is provided with a multi-layer cryopreservation rack (9). The multi-layer cryopreservation rack (9) is composed of a cryopreservation placement tray (14), an anti-detachment ring (15), an integrated bracket (16), a fixed top plate (17), a connecting rod (18), and a horizontal hanger (19). A connecting rod (18) is welded to the center of the bottom end of the horizontal hanger (19). The left and right ends of the horizontal hanger (19) can be inserted into two hanger fixing slots (12) from top to bottom respectively. The bottom end of the connecting rod (18) passes through the neck tube (11) and extends into the inner liner (8). A fixed top plate (17) is fixed to the bottom end of the connecting rod (18), and the fixed top plate (17) is located on the upper side of the center inside the inner liner (8).

7. The multilayer exosome cryopreservation device according to claim 6, characterized in that: The bottom of the left and right ends of the fixed top plate (17) is welded with an integrated bracket (16). Multiple cryopreservation trays (14) are connected at equal intervals between the two integrated brackets (16). The multiple cryopreservation trays (14) are arranged at equal intervals from top to bottom. Anti-detachment rings (15) are fixed on the outer wall of the top of the multiple cryopreservation trays (14).

8. A multilayer exosome cryopreservation device according to claim 7, characterized in that: The diameter of the fixed top plate (17) is smaller than the diameter of the neck tube (11) and the nozzle (4). After the horizontal rod (19) is horizontally fixed inside the rod fixing groove (12), multiple frozen storage trays (14) are vertically suspended in the inner liner (8). After the horizontal rod (19) is held, multiple frozen storage trays (14) can be lifted upward through the connecting rod (18) and the fixed top plate (17).

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