Cryopreservation box
By setting up flow channels inside the cryopreservation box to allow the flow of cryogenic materials, the problem of temperature rise at the bottom of the test tube during stem cell transportation was solved, ensuring the low-temperature state of stem cells and protecting cell viability.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-14
- Publication Date
- 2026-03-27
AI Technical Summary
During stem cell transport, current technology has failed to effectively maintain continuous contact between stem cells at the bottom of the test tube and dry ice, leading to increased temperature and affecting cell activity and quality.
Design a cryopreservation box with internal baffles that divide the shell into multiple layers. The baffles have notches to form flow channels, allowing low-temperature cooling materials such as dry ice to flow within the channels and ensuring continuous contact with the bottom of the cryopreservation tube.
It achieves full circulation of the cryopreservation material within the cryopreservation box, ensuring that stem cells remain at a low temperature throughout the transportation process, preventing temperature rise and protecting cell viability.
Smart Images
Figure CN224045985U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to the field of frozen transportation, specifically, a cryopreservation box. BACKGROUND
[0002] At present, stem cells are one of the research focuses in the biological field, and stem cells have extremely broad application prospects in the fields of cell repair, developmental biology, pharmacology and the like of life science.
[0003] In the stem cell transportation process, the stem cells need to be kept in a low-temperature state at all times, and currently, a method of placing the test tube containing the stem cells and dry ice in the same bag or box is usually adopted, wherein the stem cells are usually located at the bottom of the test tube, but neither the bag scheme nor the box scheme is provided with a flow channel, if the dry ice at the bottom of the test tube sublimates, the dry ice at other positions is difficult to flow to the position, which will cause the temperature of the stem cells to rise, the stem cells to be damaged, and the activity of the stem cells to be affected, thereby affecting the quality of the stem cells. CONTENT OF THE UTILITY MODEL
[0004] In view of the defects in the prior art, the utility model aims to provide a cryopreservation box.
[0005] According to the cryopreservation box provided by the utility model, the baffle is arranged in the shell body, the shell body is divided into different layers from bottom to top, the baffle is provided with a notch, the notch is used for connecting the different layers to form a flow channel, and the low-temperature cooling object is placed in the flow channel and can flow in the flow channel.
[0006] The shell body and the baffle are both provided with cryopreservation tube placing holes, and the cryopreservation tube placing holes on the shell body correspond to the cryopreservation tube placing holes on the baffle one by one.
[0007] Preferably, the number of baffles is one or more, and the multiple baffles divide the shell body into multiple layers from bottom to top.
[0008] Preferably, the cryopreservation tube placing hole is a circular hole, a square hole or a polygonal hole.
[0009] Preferably, the baffle is further provided with a through hole, and the through hole is used for connecting the different layers in the shell body.
[0010] Preferably, the diameter of the cryopreservation tube placing hole is greater than the diameter of the cryopreservation tube.
[0011] Preferably, the low-temperature cooling object is granular dry ice, and the granular dry ice can flow in the flow channel.
[0012] Preferably, the cryopreservation box is a cryopreservation box for accommodating stem cell cryopreservation tubes.
[0013] Preferably, the stem cells are located at the bottom of the cryopreservation tube, and the cryocooler is in continuous contact with the bottom of the cryopreservation tube.
[0014] Preferably, when the cryopreservation box is tilted, flipped or inverted due to external force and then returns to its upright position, the cryogenic coolant can flow along the flow channel to the bottom of the cryopreservation tube.
[0015] Preferably, it also includes an outer shell, which is a box with openings on the left and right sides, and the outer shell is installed inside the box by a pull-out method.
[0016] Compared with the prior art, the present invention has the following beneficial effects:
[0017] This invention designs the interior of the shell into different layers, which, together with the notches, form a flow channel, allowing the low-temperature cooling material and cold air inside the shell to flow and circulate fully. Even after the dry ice in contact with the bottom of the tube sublimates, the dry ice in other locations can still flow to this point, ensuring the continuous low-temperature state of the stem cells. Attached Figure Description
[0018] Other features, objects, and advantages of this invention will become more apparent from the following detailed description of non-limiting embodiments with reference to the accompanying drawings:
[0019] Figure 1 This is a schematic diagram of the structure of this utility model;
[0020] Figure 2 for Figure 1 A structural schematic diagram of the bottom view;
[0021] Figure 3 for Figure 2 A structural schematic diagram of the side view;
[0022] Figure 4 for Figure 1 A top view structural diagram;
[0023] Figure 5 for Figure 4 A structural schematic diagram of the side view;
[0024] Figure 6 This is a schematic diagram of the structure of this utility model in use.
[0025] The diagram shows:
[0026] Detailed Implementation
[0027] The utility model is described in detail below in combination with specific embodiments. The following embodiments will help the person skilled in the art to further understand the utility model, but do not limit the utility model in any form. It should be pointed out that, for those skilled in the art, without departing from the concept of the utility model, a number of changes and improvements can be made. These all belong to the protection scope of the utility model.
[0028] The utility model provides a kind of cryopreservation box, specifically, a kind of cryopreservation box for containing stem cell cryopreservation tube, stem cell is located at the bottom of cryopreservation tube, cryopreservation box can be made of plastic.
[0029] As Figures 1-6 Indicated, the cryopreservation box includes shell 1 and baffle 3, the baffle 3 is arranged in the shell 1 interior, the shell 1 interior is divided into different layers from bottom to top;The baffle 3 is provided with notch 4;The notch 4 is used to communicate different layers, forms flow channel 5;Low-temperature cooling object is placed in flow channel 5, can flow in flow channel;The shell 1 and baffle 3 are all provided with cryopreservation tube placement hole 2, the cryopreservation tube placement hole 2 on the shell 1 and the cryopreservation tube placement hole 2 on the baffle 3 correspond one by one.In one preferred embodiment, the low-temperature cooling object is granular dry ice, the granular dry ice can flow in flow channel 5, since dry ice can flow in flow channel, there is always dry ice to flow to the bottom of cryopreservation tube, that is, dry ice can continuously contact the bottom of cryopreservation tube.
[0030] The number of the baffle 3 is 1 or more, and multiple baffles 3 divide the shell 1 interior into multiple layers from bottom to top.
[0031] The cryopreservation tube placement hole 2 is a circular hole, a square hole or a polygonal hole or other shapes, and is used to accommodate the cryopreservation tube 6. In one preferred embodiment, the size of the cryopreservation tube placement hole 2 matches the size of the cryopreservation tube 6. In another preferred embodiment, the diameter of the cryopreservation tube placement hole 2 is greater than the diameter of the cryopreservation tube 6. In this preferred embodiment, the diameter of the cryopreservation tube placement hole 2 is only slightly larger than the diameter of the cryopreservation tube 6, which can ensure stable accommodation of the cryopreservation tube 6 while allowing dry ice and cold air to pass through.
[0032] In one preferred embodiment, the baffle 3 is further provided with a through hole, which is used to communicate different layers in the shell 1. The through hole allows dry ice and cold air to pass through different layers.
[0033] The cryopreservation tube placement hole 2 on the shell 1 is multiple, for example, the number of the cryopreservation tube placement hole 2 on the shell 1 is 8, and the 8 cryopreservation tube placement holes 2 are divided into 2 groups, which are arranged parallel to each other. In use, the operator can place less than the number of cryopreservation tube placement holes 2 cryopreservation tubes 6 into the cryopreservation box. At this time, the extra cryopreservation tube placement holes 2 can also allow dry ice and cold air to pass through different layers.
[0034] In a preferred embodiment, the cryo box further comprises a separate cover and a bottom plate, the cover is detachably closed on the top of the shell 1, and the bottom plate is located at the bottom of the shell 1 for supporting the cryo tube 6, and dry ice can also be placed on the bottom plate. In another preferred embodiment, the cryo box further comprises a shell, the shell is a box with openings on the left and right sides, and the shell 1 is installed in the shell by pulling.
[0035] The working principle of the utility model is as follows: when stem cells need to be transported over a long distance, the operator first loads the stem cells into a test tube (i.e. the cryo tube 6), then inserts the cryo tube 6 into the shell by using the cryo tube placement hole 2, then places low-temperature cooling substances (such as dry ice) in the flow channel 5, and finally installs the separate cover and the bottom plate or installs the shell 1 into the shell. During transportation, even if the dry ice in contact with the bottom of the test tube sublimates, the cold energy of the dry ice in other parts can still circulate through the flow channel 5 to the part where the stem cells are located (i.e. the bottom of the test tube), ensuring that the stem cells are in a low-temperature state at all times. In addition, due to the provision of the flow channel, when the cryo box is tilted, turned over or inverted due to external force and then restored to face upwards, the low-temperature cooling substances can still flow along the flow channel 5 to the bottom of the cryo tube, ensuring the low temperature of the stem cells at the bottom of the test tube.
[0036] In the description of the present application, it should be understood that the terms "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present application.
[0037] The specific embodiments of the utility model have been described above. It should be understood that the utility model is not limited to the above specific embodiments, and those skilled in the art can make various changes or modifications within the scope of the claims, which does not affect the essential content of the utility model. The embodiments of the present application and the features in the embodiments can be arbitrarily combined with each other without conflict.
Claims
1. A cryo-cassette, characterized in that, The application relates to a low-temperature cooling device for stem cell cryopreservation, which comprises a shell (1) and a baffle (3) arranged inside the shell (1) and separating the inside of the shell (1) into different layers from bottom to top; the baffle (3) is provided with a notch (4); the notch (4) is used for connecting the different layers and forming a flow channel (5); low-temperature cooling objects are placed in the flow channel (5) and can flow in the flow channel. The shell (1) and the baffle (3) are both provided with cryopreservation tube placing holes (2), and the cryopreservation tube placing holes (2) on the shell (1) correspond to the cryopreservation tube placing holes (2) on the baffle (3) one by one.
2. The cryobox of claim 1, wherein, The number of the baffles (3) is one or more, and the multiple baffles (3) separate the inside of the shell (1) into multiple layers from bottom to top.
3. The cryobox of claim 1, wherein, The cryopreservation tube placing holes (2) are circular holes, square holes or polygonal holes.
4. The cryobox of claim 1, wherein, The baffle (3) is further provided with a through hole, and the through hole is used for connecting different layers in the shell (1).
5. The cryobox of claim 1, wherein, The diameter of the cryopreservation tube placing hole (2) is greater than the diameter of a cryopreservation tube (6).
6. The cryobox of claim 1, wherein, The low-temperature cooling object is granular dry ice, and the granular dry ice can flow in the flow channel (5).
7. The cryobox of claim 6, wherein, The cryopreservation box is a cryopreservation box for containing stem cell cryopreservation tubes.
8. The cryobox of claim 7, wherein, The stem cells are located at the bottom of the cryopreservation tube, and the low-temperature cooling object continuously contacts the bottom of the cryopreservation tube.
9. The cryobox of claim 8, wherein, When the cryopreservation box is tilted, turned over or inverted due to external force and then returns to the normal position, the low-temperature cooling object can flow along the flow channel (5) to the bottom of the cryopreservation tube.
10. The cryobox of claim 1, wherein, The application further comprises a shell, which is a box body with openings on the left and right sides, and the shell is installed in the shell in a pulling manner.