Composition for relieving physical fatigue as well as preparation method and application thereof

Abstract

The invention provides a composition for relieving physical fatigue as well as a preparation method and application thereof, and relates to the technical field of medicines and foods. The composition mainly comprises the following components in parts by weight: 10-25 parts of soybean peptide, 20-50 parts of whey protein powder, 10-25 parts of sheep milk powder, 2-10 parts of leucine, 0.5-4 parts of acerola cherry powder, 0.1-2 parts of B-enriched yeast, 2-8 parts of fructo-oligosaccharide and 3-10 parts of red date powder. According to the composition disclosed by the invention, all the components have a synergistic effect, so that not only can energy and nutrient substances consumed by exercise be quickly supplemented and fatigue be relieved, but also muscle synthesis can be promoted, exercise performance can be enhanced, and comprehensive recovery after exercise can be effectively promoted.

Description

TECHNICAL FIELD

[0001] The present application relates to the fields of medicine and food technology, in particular to a composition for relieving physical fatigue and a preparation method and application thereof. BACKGROUND

[0002] With the improvement of people's living standards and the change of concept, national fitness gradually leads to a craze, and exercise has become an important way for people to pursue physical health, enhance self-confidence, relax mood and enhance social vitality. However, during the exercise process, the continuous excitement of the brain, the accumulation of muscle metabolites and the consumption of glycogen lead to fatigue, muscle soreness and even micro-injury after exercise. How to effectively recover physical strength, relieve fatigue, repair muscle tissue and reduce discomfort after exercise has become the focus of attention of the exercise population.

[0003] Muscle fatigue refers to the phenomenon of decrease in maximum force and contraction speed generated by muscle contraction, mainly manifested as physical fatigue, reduced work efficiency, inattention, slow movement, reduced coordination and flexibility, etc. The causes of muscle fatigue can be explained by the following three theories: central theory believes that the relevant regions of the brain are continuously excited, thereby producing inhibitory protection and inhibiting the spread of related factors in the brain, leading to the generation of muscle fatigue; metabolite accumulation theory believes that the body continuously produces and accumulates metabolites such as lactic acid during exercise, which produces a toxic effect, thereby causing fatigue; energy theory believes that continuous exercise will consume the energy substances such as muscle glycogen in the body, hindering the energy supply of muscles, brain and other tissues and organs, causing people to feel tired and fatigued.

[0004] Reasonable rest is an important way to eliminate muscle fatigue. In order to adapt to the fast-paced modern life, people pursue a faster and more effective fatigue elimination method while improving exercise capacity. Although there are some products on the market for post-exercise physical recovery, most of the products only stimulate the central nervous system through ingredients such as caffeine and taurine to achieve rapid relief of fatigue, and the efficacy is single, which cannot meet the comprehensive recovery needs of the exercise population. For example, a certain brand of functional beverage, the main raw materials of which are taurine, gluconic acid, nicotinamide, etc., can quickly relieve physical fatigue by supplementing water and electrolytes and improving body excitability. Such products can only improve the fatigue state in a short time, and cannot continuously relieve muscle soreness and improve exercise capacity. In addition, for the exercise population, it is necessary to use raw materials that can easily excite people and have the possibility of addiction with caution. SUMMARY

[0005] Therefore, the embodiments of the present application provide a composition for relieving physical fatigue and a preparation method and application thereof. The composition has a synergistic effect between the components, can not only quickly supplement the energy and nutrients consumed during exercise and relieve fatigue, but also promote muscle synthesis, enhance exercise performance, and effectively promote comprehensive recovery after exercise.

[0006] The embodiment of the present application provides the following technical scheme: a composition for relieving physical fatigue, main components in the composition are composed of the following components in parts by weight: 10-25 parts of soybean peptide, 20-50 parts of whey protein powder, 10-25 parts of sheep milk powder, 2-10 parts of leucine, 0.5-4 parts of needle leaf cherry powder, 0.1-2 parts of B-rich yeast, 2-8 parts of fructooligosaccharide and 3-10 parts of red date powder; wherein the whey protein powder, the soybean peptide and the sheep milk powder are used in a ratio of 2:1:1, and the whey protein powder and the leucine are added in a ratio of (3-8):1.

[0007] The present application provides a composition for relieving physical fatigue, which is mainly composed of soybean peptide, concentrated whey protein powder, sheep milk powder, leucine, needle leaf cherry powder, B-rich yeast, fructooligosaccharide and red date powder, and can more comprehensively and effectively relieve physical fatigue after exercise, and safely and continuously enhance exercise performance through scientific proportioning.

[0008] The high-quality animal and plant proteins, polypeptides and amino acids necessary for muscle protein synthesis are rapidly and continuously provided through the synergistic effect of the soybean peptide, the concentrated whey protein powder, the sheep milk powder and the leucine, the muscle consumption is rapidly supplemented, the physical fatigue after exercise is relieved, the muscle damage is reduced, the muscle synthesis raw materials are continuously provided, and the exercise performance is enhanced.

[0009] The B-rich yeast powder provides natural whole B vitamins, promotes protein synthesis and inhibits protein decomposition at the same time, increases muscle synthesis, the needle leaf cherry powder provides natural Vc, reduces muscle pain, inhibits the inflammatory response of the body, reduces muscle damage caused by exercise, and improves the fatigue symptoms after exercise.

[0010] The addition of fructooligosaccharide improves the intestinal tract and prevents constipation.

[0011] The red date powder is selected for seasoning, effectively improves the inherent bitter taste of the soybean peptide, and the red date has a certain blood-nourishing and tranquilizing effect, and helps to relieve the mental stress state after fatigue.

[0012] The addition ratio of the whey protein powder, the soybean peptide and the sheep milk powder in the embodiment is about 2:1:1, and the animal protein and the plant protein are combined, so that the nutritional complementation and the advantage superposition are realized.

[0013] According to one embodiment of the present application, main components in the composition are composed of the following components in parts by weight: 15-20 parts of soybean peptide, 30-40 parts of whey protein powder, 15-20 parts of sheep milk powder, 5-10 parts of leucine, 1-3.5 parts of needle leaf cherry powder, 1-2 parts of B-rich yeast, 5-8 parts of fructooligosaccharide and 5-10 parts of red date powder.

[0014] According to one embodiment of the present application, the main components in the composition are composed of the following components by weight parts: 20 parts of soybean peptide, 40 parts of whey protein powder, 20 parts of sheep milk powder, 5 parts of leucine, 2 parts of pinus koraiensis powder, 1 part of B-rich yeast, 6 parts of fructo-oligosaccharide, and 6 parts of red date powder.

[0015] According to one embodiment of the present application, the main components in the composition are composed of the following components by weight parts: 20 parts of soybean peptide, 40 parts of whey protein powder, 20 parts of sheep milk powder, 5 parts of leucine, 2 parts of pinus koraiensis powder, 1 part of B-rich yeast, 6 parts of fructo-oligosaccharide, and 6 parts of red date powder.

[0016] According to one embodiment of the present application, the main components in the composition are composed of the following components by weight parts: 20 parts of soybean peptide, 40 parts of whey protein powder, 20 parts of sheep milk powder, 5 parts of leucine, 2 parts of pinus koraiensis powder, 1 part of B-rich yeast, 6 parts of fructo-oligosaccharide, and 6 parts of red date powder.

[0017] According to one embodiment of the present application, the composition further comprises auxiliary materials, which include any one or more of radix codonopsis, angelica sinensis and astragalus membranaceus.

[0018] According to one embodiment of the present application, the whey protein powder is concentrated whey protein powder or separated whey protein powder.

[0019] The present application further provides a preparation method of the composition for relieving physical fatigue, which comprises: uniformly mixing 10-25 parts of soybean peptide, 20-50 parts of whey protein powder, 10-25 parts of sheep milk powder, 2-10 parts of leucine, 0.5-4 parts of pinus koraiensis powder, 0.1-2 parts of B-rich yeast, 2-8 parts of fructo-oligosaccharide, and 3-10 parts of red date powder by weight parts to obtain the composition.

[0020] The present application further provides an application of the composition as described above in the preparation of food, medicine or health food for relieving physical fatigue.

[0021] The composition of the present application has a significant function of relieving physical fatigue. The functions of the components are as follows: (1) Soybean peptide: It is the hydrolyzate of high-quality plant protein soybean protein. Soybean peptide contains 18 kinds of amino acids, including 8 kinds of essential amino acids. Except for methionine as a limiting amino acid, other amino acids are more than or close to the recommended standard of the World Health Organization. A large number of studies have shown that many amino acids are absorbed in the form of small peptides (oligopeptides composed of 2-6 amino acids). The digestion end product of protein in the digestive tract is mostly oligopeptide rather than free amino acid. Small peptides can enter the body circulation through the intestinal mucosa cells completely. Soybean peptide has good essential amino acid balance, and its nutritional value is equivalent to that of animal-derived oligopeptide. Soybean peptide can also destroy antigenic determinants and reduce human allergic reactions. Small molecule peptides are more easily absorbed by the human body. The gastric digestibility of soybean peptide powder is 81.61%, the intestinal digestibility is 87.49%, and the absorption peak reaches the top at 30 min, which is more suitable for people in a stressful state.

[0022] (2) Concentrated whey protein powder: Studies have shown that during the entire gastric digestion process, whey protein (WP) forms more soluble aggregates in the stomach. This makes the gastric emptying rate faster, and the protein is more easily hydrolyzed in the intestine, thereby promoting protein absorption, so it is known as “fast protein”. The gastric emptying time of WP is about 150 min, and the digestibility is 91.7%. As a kind of “fast protein”, the intake of WP will lead to faster protein digestion and amino acid absorption kinetics, which has special effects on stimulating muscle protein synthesis and promoting postprandial insulin release. The content of leucine in WP is higher, so the concentration of leucine in the blood after a meal rises faster. Leucine is an important regulator of postprandial muscle protein metabolism, which will increase the synthesis rate of muscle protein in the body. When WP supplementation is combined with resistance exercise, it will further stimulate the synthesis of muscle protein in muscle fibers.

[0023] The concentrated whey protein powder WPC 80 selected in the application adopts a medium-low temperature spray drying process and has the following advantages: no addition of lecithin or any other auxiliary materials and additives, pure and correct milk aroma; single cheese whey, single production plant, high batch stability; good powder flowability and water solubility are achieved by adopting an agglomeration process. Concentrated whey protein powder has the characteristics of high protein, easy absorption and high bioavailability, contains various essential amino acids, minerals, vitamins and active substances required by the human body, and plays an important role in human body metabolism balance, immunity, disease prevention and treatment, and is an important raw material for producing infant formula milk powder, sports nutrition supplements, and nutritional products for the middle-aged and the elderly.

[0024] (3) Sheep milk powder: Its protein, lactoferrin and calcium content can reach twice that of cow's milk, rich in multiple vitamins and minerals, more than 10 kinds of essential amino acids for the human body; And its milk fat molecular particle size is smaller, the probability of combining with lipase is greater (6% smaller than goat milk, 27.5% smaller than cow milk); The proportion of short-chain and medium-chain fatty acids is higher, which is easy to be hydrolyzed by lipase, and the digestion and absorption rate is higher; The time through the gastrointestinal system is faster, reducing symptoms such as bloating. Sheep milk is a natural full-A2 type dairy product, which does not contain α s1 -casein, which is not easy to cause gastrointestinal cramps and abdominal distension and other gastrointestinal discomfort.

[0025] In addition, sheep milk is rich in medium-chain TAG (containing at least 2 medium-chain fatty acids, 24.1%) and medium-chain fatty acids (14.5%-27.6%). Medium-chain TAG is a fast energy source, which can be digested and released in the stomach, and medium-chain fatty acids can be transported to the liver through the portal vein and rapidly decomposed for energy supply. High-pressure homogenization and spray drying are the most common dairy processing technologies, which can affect the particle size, interface composition and properties of fat globules. Changes in these physicochemical properties may affect its intestinal digestibility, thereby affecting milk fat bioavailability, absorption and postprandial metabolism.

[0026] (4) Leucine: an amino acid with significant effects on stimulating protein synthesis and slowing down protein decomposition. For athletes, leucine can effectively inhibit protein decomposition in the body, and supplementing 150 mg / kg of leucine can also reduce skeletal muscle damage and pain.

[0027] (5) Acerola cherry powder: rich in vitamin C, which plays an important role in enhancing immunity, promoting protein synthesis, and protecting cells from oxidative damage. Exercise causes excessive production of reactive oxygen species (ROS) in muscle tissue, causing oxidative-antioxidant system disorder, forming muscle oxidative stress, and vitamin C helps to remove ROS, regulate the expression of muscle tissue-related proteins, reduce oxidative stress and inflammatory response, and promote muscle tissue repair and regeneration. In addition, acerola cherry also contains vitamin A, vitamin E, calcium, iron, potassium and other nutrients.

[0028] (6) B-rich yeast powder: cultured from natural yeast, with good flavor, complete types of B vitamins, high content and high bioavailability, which is the necessary coenzyme for various biochemical reactions in the human body. For example, vitamin B6 is involved in the metabolism of all amino acids and protein synthesis, and is the only coenzyme for various amino acid metabolism, which plays an important role in the process of glycogen decomposition, protein and lipid metabolism in the human body.

[0029] (7) Fructooligosaccharides: a high-quality dietary fiber that can improve the intestinal environment and improve nutrient absorption capacity, and also has certain antioxidant effects.

[0030] Compared with the prior art, the composition of the present application has the following advantages: (1) Rapidly providing raw materials for muscle protein synthesis: The soybean peptides, concentrated whey protein powder, sheep milk powder and leucine in the present application jointly provide high-quality animal and plant proteins, polypeptides and amino acids necessary for muscle protein synthesis. During gastric digestion, whey protein forms a large number of soluble aggregates in the stomach, which accelerates the gastric emptying rate and makes the protein more easily enter the intestine for hydrolysis, thereby promoting protein absorption, and thus whey protein is honored as "fast protein". Its intake leads to faster protein digestion and amino acid absorption kinetics, and has special effects on stimulating muscle protein synthesis of muscle fibers and promoting postprandial insulin release. Soybean peptides are hydrolyzates of soybean protein, have small molecules and higher absorption and utilization rates, can quickly pass through the intestinal mucosa into the blood, and improve the utilization rate of nutrients such as protein and minerals, and also have some unique physiological activities. The above ingredients jointly act to rapidly replenish muscle consumption, relieve post-exercise fatigue, continuously provide raw materials for muscle synthesis, and enhance exercise performance.

[0031] (2) Synergistic effect to promote muscle protein synthesis: Leucine is an important regulator of postprandial muscle protein metabolism, which increases the muscle protein synthesis rate of the body. Vitamin B6 is an important coenzyme in the amino acid metabolic pathway, which is involved in the metabolism of all amino acids and protein synthesis. The supplementation of other proteins and polypeptides increases the level of free amino acids in the blood, which promotes protein synthesis and inhibits protein decomposition.

[0032] (3) Reducing muscle damage: Centrifugal exercise can cause structural muscle damage and pain, and muscle fibers respond to mechanical damage by releasing pro-inflammatory cytokines. Timely supplementation of antioxidants after high-intensity centrifugal exercise can effectively restore muscle function and relieve muscle soreness. In addition, the supplementation of protein can increase the proliferation of satellite cells after the recovery of centrifugal exercise. The concentrated whey protein powder and vitamin C provided by the needle cherry in the present application jointly act to effectively reduce muscle damage caused by exercise, inhibit the inflammatory response of the body, reduce the degree of muscle soreness, and the effect of the combination of the two is superior to the supplementation of protein alone.

[0033] (4) Regulating gastrointestinal function: The proportion of short-chain and medium-chain fatty acids in sheep milk powder is higher, which is easily hydrolyzed by lipase, has higher digestion and absorption rates, and passes through the gastrointestinal system faster, which can effectively reduce symptoms such as bloating. A large amount of protein intake can easily cause a burden on the gastrointestinal tract, and oligofructose as a high-quality dietary fiber can promote gastrointestinal peristalsis and enhance the function of nutrient absorption.

[0034] (5) high safety, excellent flavor, simple process: all components of the application are of natural origin. Red jujube powder and needle leaf cherry powder are selected for joint seasoning, effectively improving the inherent bitter taste of soybean peptide, and red jujube powder is rich in various trace elements and also has the effect of nourishing blood and calming the nerves, which helps to relieve the mental stress after fatigue. The preparation method of the application is simple and easy to operate, and is suitable for large-scale industrial production. DETAILED DESCRIPTION

[0035] The embodiments of the application are described in detail below.

[0036] The embodiments of the application are described below by specific examples, and those skilled in the art can easily understand other advantages and effects of the application from the disclosure. Obviously, the described embodiments are only a part of the embodiments of the application, not all. The application can also be implemented or applied by other different specific embodiments, and various modifications or changes can be made to the details in the specification without departing from the spirit of the application. It should be noted that the following embodiments and features in the embodiments can be combined with each other without conflict. Based on the embodiments in the application, all other embodiments obtained by those skilled in the art without creative labor are within the scope of protection of the application.

[0037] In the following embodiments of the application, the concentrated whey protein powder is purchased from Yilin International Trade (Guangzhou) Co., Ltd., produced in Australia, and the brand is SDA (Saputo); the soybean peptide is purchased from Zhejiang Kangyuan, produced in China; the sheep milk powder is purchased from Yilin International Trade (Guangzhou) Co., Ltd., produced in New Zealand, and the brand is Spring Sheep (Chunmian); the leucine is purchased from Dalian Yinao, produced in China; the needle leaf cherry powder is purchased from Yilin International Trade (Guangzhou) Co., Ltd., produced in Brazil, and the brand is Shuanglun; the B-rich yeast powder is purchased from Yilin International Trade (Guangzhou) Co., Ltd., produced in Germany, and the brand is BASF; the fructooligosaccharide is purchased from Quantum High-tech, produced in China; and the red jujube powder is purchased from Haixiangni, produced in China.

[0038] Embodiment 1 Soybean peptide 200 g, concentrated whey protein powder 400 g, sheep milk powder 200 g, leucine 60 g, needle leaf cherry powder 20 g, B-rich yeast 10 g, fructooligosaccharide 60 g, and red jujube powder 80 g are weighed and uniformly mixed, packaged, and 1000 g of composition finished product is obtained.

[0039] Embodiment 2 Take 160 g of soybean peptide, 320 g of concentrated whey protein powder, 160 g of sheep milk powder, 100 g of leucine, 30 g of needle cherry powder, 20 g of B-rich yeast, 80 g of fructooligosaccharide, and 100 g of red jujube powder, mix them evenly, package, and obtain 1000 g of composition finished product.

[0040] Example 3 Take 200 g of soybean peptide, 400 g of concentrated whey protein powder, 200 g of sheep milk powder, 50 g of leucine, 20 g of needle cherry powder, 10 g of B-rich yeast, 60 g of fructooligosaccharide, and 60 g of red jujube powder, mix them evenly, package, and obtain 1000 g of composition finished product.

[0041] Preferably, in the embodiments of the present application, the addition ratio of whey protein powder, soybean peptide and sheep milk powder is about 2:1:1, which has both animal protein and plant protein, realizes nutritional complementation and advantage superposition. The rationality of the protein ratio in the present formula is evaluated according to the amino acid score. The results are shown in Table 1.

[0042] Table 1 Amino acid score table

[0043] As can be seen from Table 1, through compounding, the amino acid score (AAS) of the mixed protein is slightly lower than that of concentrated whey protein powder and sheep milk powder, but is much higher than that of soybean peptide. Soybean peptide provides high-quality plant protein in the present formula, and peptides are more easily absorbed by the human body, have unique physiological activity, and can achieve a higher amino acid score after compounding. The amino acid score ratio coefficient (SRCAA) of the mixed protein is significantly improved compared with single protein, which can play a complementary role in protein, and the nutritional value of the mixed protein is significantly enhanced. If the compounding ratio of concentrated whey protein powder or sheep milk powder is increased, the SRCAA score decreases, which is presumably due to the fact that the content of individual amino acids is too high or too low, resulting in a large degree of dispersion of the amino acid composition. Moreover, the amino acid composition after compounding is closer to the WHO recommended mode and is more easily absorbed by the human body.

[0044] Taking into account the amino acid score, the amino acid score ratio coefficient, the content of essential amino acids per gram of protein, and the digestion and absorption rate, the concentrated whey protein powder: soybean peptide: sheep milk powder is preferably compounded at a ratio of 2:1:1. Leucine is an important regulator of postprandial muscle protein metabolism, which increases the body's muscle protein synthesis rate, and leucine is additionally added in the present formula.

[0045] The composition prepared in the above manner is taken as the research object, and according to the method for evaluating the function of relieving physical fatigue in the Health Food Function Evaluation Method (2023 Edition), the effects of the composition on the weight-loaded swimming time of mice, blood urea, liver glycogen content, and blood lactic acid curve area are investigated.

[0046] Experiment 1 1.1 Experimental conditions and methods (1) Experimental animals SPF level KM mice were purchased from Sibeifu (Beijing) Biotechnology Co., Ltd. Production license number: SCXK (Jing) 2019-0010, issued by Beijing Municipal Science and Technology Commission. This unit's experimental animal use license number: SYXK (Dian) K2022-0002, issued by Kunming Municipal Science and Technology Bureau.

[0047] (2) Reagents Urea nitrogen assay kit, blood lactic acid assay kit, glycogen assay kit, item numbers: C013-2-1, A019-1-1, A043-1-1, batch numbers: 20240131, all produced by Nanjing Jiancheng Biological Engineering Institute; isoflurane, produced by Lunan Bet Pharmaceutical Co., Ltd., batch number: 64150702; pure water, provided by the Pharmacological Laboratory of Yunnan Institute of Pharmaceutical Research ELGA LA613 ultrapure water machine.

[0048] (3) Instruments JJ2000 electronic balance, Changshu Shuangjie Testing Instrument Factory; Practum224 SQP electronic analytical balance, Sartorius Scientific Instruments (Beijing) Co., Ltd.; Multiskan GO full-wavelength enzyme marker, Thermo Fisher Scientific Company; 3K15 desktop refrigerated high-speed centrifuge, Germany Sigma Company; ELGA LA613 ultrapure water machine, UK ELGA LabWater / VWS (UK) Ltd.

[0049] (4) Animal grouping Take 48 SPF KM mice, all male, and divide them into 4 groups according to body weight, namely the solvent control group, the composition 4.17, 8.33, 16.67 g / kg dose groups of Example 3 of the present application, each group of 12 mice. The composition of the present application is administered by gavage with the corresponding dose sample every day, and the solvent control group is given the same volume of pure water, 1 time / d, for 30 consecutive days. The animals in each group are weighed twice a week.

[0050] (5) Weighted swimming experiment After the last gavage administration of the test sample for 30 min, the mice with a 5% body weight lead bar at the root of the tail were placed in the swimming box for swimming. The water depth is not less than 30 cm, and the water temperature is (25±1.0)℃. The time from the start of swimming to death of the mouse, i.e. the weighted swimming time of the mouse, was recorded.

[0051] (6) Serum urea determination experiment After the last intragastric administration of the test sample 30 min, the mice were not loaded swimming in water with temperature of (30±1.0)℃ for 90 min, and after resting for 60 min, the animals were anesthetized, 1 mL of blood was collected from inferior vena cava using heparin anticoagulation tube, and the plasma was separated by centrifugation at 3000 r / min for 15 min. The blood urea content in the plasma was detected according to the method of urea nitrogen determination kit instruction.

[0052] (7) Hepatic glycogen determination experiment After the last intragastric administration of the test sample 30 min, the animals were sacrificed, the liver was rinsed with normal saline and then dried with filter paper, 75 mg of liver was accurately weighed, and the glycogen content in the liver was detected according to the method of glycogen determination kit instruction.

[0053] (8) Blood lactic acid determination experiment After the last intragastric administration of the test sample 30 min, 50 μL of blood was collected from the tail tip of each group of animals, and then the animals were not loaded swimming in water with temperature of (30±1.0)℃ for 10 min, 50 μL of blood was immediately collected, and 50 μL of blood was collected again after resting for 20 min, and the blood lactic acid (LD) content at the three time points was detected according to the method of blood lactic acid determination kit instruction. The area under the blood lactic acid curve was calculated according to the following formula: Area under the blood lactic acid curve =

[0054] (9) Statistical method The experimental results are represented by , and the data are analyzed by SPSS 22.0 statistical software. After normality test and variance homogeneity test, the data meet the requirements of normal distribution and variance homogeneity, and one-way analysis of variance is performed, and the differences between groups are compared by LSD method, and the test level is α=0.05. For non-normal or non-homogeneous data, appropriate variable transformation is performed, and after meeting the requirements of normality and variance homogeneity, the transformed data are used for statistics; if the variable transformation still does not meet the requirements of normality or variance homogeneity, the rank sum test is used for statistics.

[0055] (10) Result determination Weight-loaded swimming experiment: the weight-loaded swimming time of any test sample group is significantly longer than that of the solvent control group, and the difference is significant, which can be determined as positive result.

[0056] Blood urea determination: the blood urea of any test sample group is lower than that of the solvent control group, and the difference is significant, which can be determined as positive result.

[0057] Hepatic glycogen determination: the hepatic glycogen content of any test sample group is significantly higher than that of the solvent control group, and the difference is significant, which can be determined as positive result.

[0058] Blood lactate assay: The area under the blood lactate curve at three time points is used for judgment. If the area under the curve of any test sample group is smaller than that of the solvent control group, and the difference is statistically significant, the experimental result can be judged as positive.

[0059] If the weighted swimming test result is positive, and any two of the three biochemical indicators—blood lactate, serum urea, and liver glycogen—are positive, it can be determined that the test sample has the effect of relieving physical fatigue.

[0060] 1.2 Experimental Results (1) Results of the weighted swimming experiment Compared with the solvent control group, after continuous gavage administration of the test sample for 30 days, the weight-bearing swimming time of mice in the 4.17 g / kg dose group of the composition of the present invention was significantly increased. P <0.05); the composition of the present invention had no significant effect on the body weight of mice at any dosage group ( P >0.05), the results are shown in Table 2. The mouse weight-bearing swimming test result of the composition of the present invention in this experiment was determined to be positive.

[0061] Table 2. Effects of the composition of the present invention on the weight-bearing swimming time of mice ( (n=12)

[0062] Note: Compared with the solvent control group. *P <0.05.

[0063] (2) Serum urea test results Compared with the solvent control group, after continuous gavage administration of the test sample for 30 days, the serum urea content in mice in the 16.67 g / kg dose group of the composition of the present invention was significantly increased. P <0.01%, and the other dosage groups had no significant effect on the serum urea content of mice. P >0.05); the composition of the present invention had no significant effect on the body weight of mice at any dosage group ( P >0.05), the results are shown in Table 3. The result of this experiment regarding the effect of the composition of the present invention on serum urea was negative.

[0064] Table 3 Effects of the composition of the present invention on serum urea levels in mice ( (n=12)

[0065] Note: Compared with the solvent control group. **P <0.01.

[0066] (3) Results of liver glycogen measurement Compared with the solvent control group, the liver glycogen content of the mice in the 4.17 g / kg and 8.33 g / kg dose groups of the composition of the present application was significantly increased after continuous intragastrical administration of the test sample for 30 days P <0.05 or P <0.01); the composition of the present application had no significant effect on the body weight of the mice in each dose group P >0.05), and the results are shown in Table 4. The effect of the composition of the present application on liver glycogen in this experiment was determined to be positive.

[0067] Table 4 Effect of the composition of the present application on the liver glycogen content of mice , n = 12

[0068] Note: compared with the solvent control group, *P <0.05, **P <0.01.

[0069] (4) Blood lactic acid determination results Compared with the solvent control group, the area under the curve of blood lactic acid of the mice in the 16.67 g / kg dose group of the composition of the present application was significantly reduced after continuous intragastrical administration of the test sample for 30 days P <0.01); the composition of the present application had no significant effect on the body weight of the mice in each dose group P >0.05), and the results are shown in Table 5. The effect of the composition of the present application on blood lactic acid in this experiment was determined to be positive.

[0070] Table 5 Effect of the composition of the present application on the area under the curve of blood lactic acid of mice , n = 12

[0071] Note: compared with the solvent control group, **P <0.01.

[0072] According to the result determination standard, the mouse weight-loaded swimming experiment result of the composition of the present application was positive, and the effect on the two biochemical indicators of liver glycogen and blood lactic acid was positive, so it can be determined that the composition of the present application is applied to food, medicine or health food, and animal experiments have proved that it has a good effect on relieving physical fatigue.

[0073] Experiment 2 2.1 Experimental materials and grouping Experimental animals: 150 healthy male ICR mice, weighing 18-22 g, were randomly divided into 10 groups, 15 mice in each group.

[0074] A, blank control group: intragastrical administration of equal volume of normal saline.

[0075] B. Leucine-free group: Intragastrically administered with the basic composition without leucine (whey protein powder, soybean peptide, sheep milk powder were mixed at a ratio of 2:1:1, containing all ingredients except leucine).

[0076] C. Whey protein-free group: Intragastrically administered with the basic composition without whey protein (soybean peptide, sheep milk powder were adjusted in proportion, containing all ingredients except whey protein).

[0077] D. Soybean peptide-free group: Intragastrically administered with the basic composition without soybean peptide (whey protein powder, sheep milk powder were adjusted in proportion, containing all ingredients except soybean peptide).

[0078] E. Leucine monomer group: Intragastrically administered with an equal amount of leucine as the complete formula group.

[0079] F. Leucine first formula group: Intragastrically administered with a mixture of whey protein powder and leucine (whey protein powder: leucine = 5:1).

[0080] G. Leucine second formula group: Intragastrically administered with a mixture of soybean peptide and leucine (soybean peptide: leucine = 5:1).

[0081] H. Leucine third formula group: Intragastrically administered with a mixture of sheep milk powder and leucine (sheep milk powder: leucine = 5:1).

[0082] I. Complete formula group: Intragastrically administered with the complete composition containing leucine (whey protein powder: leucine = 5:1).

[0083] J. Positive control group: Existing anti-fatigue health care product on the market (main ingredient is taurine and B vitamins).

[0084] Dose: All test substances were administered to mice at 10 times the recommended human dose (converted according to body surface area), once a day by intragastric administration, for 28 consecutive days.

[0085] The formula composition of the above complete formula group: whey protein powder: 32.5 parts, soybean peptide: 16.25 parts, sheep milk powder: 16.25 parts, leucine: 6.5 parts, other ingredients (acerola powder, B-rich yeast, fructooligosaccharide, red jujube powder) were added in proportion.

[0086] 2.2 Experimental method After the last administration for 30 minutes, the mice were subjected to the forced swimming test.

[0087] Exhaustive swimming time: The time from the start of swimming to the mouse's head being submerged in water for 10 seconds and unable to float to the surface was recorded as a direct anti-fatigue ability indicator.

[0088] Post-exercise recovery indicators: Another batch of mice were subjected to quantitative load swimming (5% body weight, swimming for 60 minutes), and blood and muscle tissue samples were collected at 0, 30 and 60 minutes after exercise.

[0089] Blood lactate (BLA): Blood lactate level and clearance rate after exercise are the core indicators for evaluating fatigue recovery.

[0090] Blood urea nitrogen (BUN): Reflects the degree of protein catabolism and fatigue condition during exercise.

[0091] Liver glycogen (LG): An important energy reserve for exercise tolerance.

[0092] 2.3 Simulation experiment results and analysis Table 6: Comparison of exhaustive swimming time of mice in each group (n=10)

[0093] By comparison, the mice in the complete formula group had the strongest exercise tolerance, and the exhaustive swimming time was significantly longer than that of the other groups. The removal of any key ingredient (leucine, whey protein, soy peptide) significantly reduced the endurance effect, among which the absence of leucine had the greatest impact on endurance, and the addition of leucine improved endurance by nearly 50%, proving the key role of leucine in improving extreme exercise capacity. The effect of leucine and single protein source complex group was significantly better than that of leucine monomer group and the corresponding missing group, which proved that leucine and any protein source selected by the present application could produce clear synergistic effect. Among them, the whey protein and leucine complex had the best effect, highlighting the synergistic effect of whey protein as a "fast protein" and leucine in rapidly promoting synthesis and improving endurance.

[0094] Table 7: Blood lactate (BLA) clearance of mice in each group after quantitative load exercise (mmol / L, n=10)

[0095] By comparison, there was no significant difference in blood lactic acid level at the end of exercise, indicating that the exercise load was consistent. During the recovery period, the blood lactic acid clearance rate of the complete formula group was much faster than that of the other groups. At 30 minutes and 60 minutes after exercise, the blood lactic acid levels were significantly lower than those of the key component missing group, the monomer group and the positive control group. After removing whey protein or soy peptide, the blood lactic acid clearance capacity decreased significantly, indicating that the rapid absorption of whey protein and the sustained energy supply of soy peptide are crucial to lactic acid metabolism. The component of removing leucine decreased most significantly, which indicated that the composition added with leucine could greatly accelerate lactic acid metabolism and quickly eliminate fatigue. The lactic acid clearance capacity of the leucine and single protein source compound group was significantly enhanced, and the effect was obviously better than that of the monomer group and the corresponding missing group. Among them, the clearance rate of whey protein + leucine was the fastest, and the effect was close to that of the positive control group, indicating that whey protein has a unique advantage in promoting lactic acid metabolism.

[0096] Table 8: Comparison of biochemical indicators of mice in each group at 60 minutes after exercise , n=10)

[0097] By comparison, the blood urea nitrogen level of the complete formula group was the lowest, indicating that the formula combination can effectively inhibit protein decomposition during exercise, reduce the production of nitrogen-containing waste, and reduce fatigue from the source. The liver glycogen reserve of the complete formula group was the highest, and the liver glycogen reserve decreased after removing whey protein or soy peptide, indicating that the two have a synergistic effect in energy supplementation. The blood urea nitrogen increased significantly after removing leucine, indicating that leucine plays a key role in inhibiting protein decomposition; the liver glycogen data indicates that leucine and the carbohydrates (fructooligosaccharides) in the formula synergize to promote the rapid recovery of energy reserves after exercise, providing energy security for subsequent activities. Leucine and single protein source compound group showed a significant synergistic effect in reducing blood urea nitrogen and increasing liver glycogen, and the effect was much better than that of the leucine monomer group. Among them, whey protein + leucine had the most outstanding effect, indicating that the combination has a strong synergistic effect in promoting anabolism and energy recovery. Therefore, the physical fatigue relieving composition of the present application has excellent anti-fatigue and recovery promoting effect, can significantly prolong the exhaustive swimming time of mice, accelerate blood lactic acid clearance, reduce protein decomposition, and increase energy reserves. Leucine plays a key role in the formula, and by compounding with whey protein powder in a scientific ratio, the performance of the basic formula is significantly improved. In addition, leucine, as a branched chain amino acid, can not only be directly used by muscles as energy to reduce protein decomposition, but more importantly, it can efficiently activate the mTOR signaling pathway to directly promote muscle protein synthesis, thereby accelerating the repair and regeneration of muscle fibers after exercise. This forms a multi-target, synergistic effect with the rapid absorption of amino acids provided by whey protein powder, the sustained release of amino acids provided by soy peptide and sheep milk powder, and the energy and antioxidant support provided by fructooligosaccharides and needle cherry powder.

[0098] The above experiment shows that the specific ratio of "whey protein: soybean peptide: sheep milk powder = 2:1:1", scientific addition of leucine and other auxiliary ingredients (needle cherry powder, B-rich yeast, etc.), the multi-component and multi-target nutritional support system constructed by the application, the comprehensive effect is incomparable to single or binary combination, and the comprehensive synergistic effect of "1+1+1>3" is realized.

[0099] The above is only a specific embodiment of the present application, but the protection scope of the present application is not limited to this. Any person skilled in the art can easily think of changes or replacements within the technical range disclosed in the present application, which should be covered within the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the protection scope of the claims.

Claims

1. A composition for relieving physical fatigue, characterized in that, The main components of the composition are composed of the following components in parts by weight: The ingredients are: 10-25 parts soybean peptide, 20-50 parts whey protein powder, 10-25 parts sheep milk powder, 2-10 parts leucine, 0.5-4 parts acerola cherry powder, 0.1-2 parts B-enriched yeast, 2-8 parts fructooligosaccharides, and 3-10 parts jujube powder. The whey protein powder, soybean peptide, and sheep milk powder are mixed in a ratio of 2:1:1, and the whey protein powder and leucine are added in a ratio of (3-8):

1.

2. The composition for relieving physical fatigue according to claim 1, characterized in that, The main components of the composition are composed of the following components in parts by weight: Soybean peptides 15-20 parts, whey protein powder 30-40 parts, sheep milk powder 15-20 parts, leucine 5-10 parts, acerola cherry powder 1-3.5 parts, B-rich yeast 1-2 parts, fructooligosaccharides 5-8 parts, jujube powder 5-10 parts.

3. The composition for relieving physical fatigue according to claim 1, characterized in that, The main components of the composition are composed of the following components in parts by weight: 20 parts soybean peptides, 40 parts whey protein powder, 20 parts sheep milk powder, 5 parts leucine, 2 parts acerola cherry powder, 1 part B-rich yeast, 6 parts fructooligosaccharides, and 6 parts jujube powder.

4. The composition for relieving physical fatigue according to claim 1, characterized in that, The main components of the composition are composed of the following components in parts by weight: 20 parts soybean peptides, 40 parts concentrated whey protein powder, 20 parts sheep milk powder, 6 parts leucine, 2 parts acerola cherry powder, 1 part B-rich yeast, 6 parts fructooligosaccharides, and 8 parts jujube powder.

5. The composition for relieving physical fatigue according to claim 1, characterized in that, The main components of the composition are composed of the following components in parts by weight: 16 parts soybean peptides, 32 parts concentrated whey protein powder, 16 parts sheep milk powder, 10 parts leucine, 3 parts acerola cherry powder, 2 parts B-rich yeast, 8 parts fructooligosaccharides, and 10 parts jujube powder.

6. The composition for relieving physical fatigue according to claim 1, characterized in that, The composition also includes excipients, which include one or more of Codonopsis pilosula, Angelica sinensis, and Astragalus membranaceus.

7. The composition for relieving physical fatigue according to claim 1, characterized in that, The whey protein powder used is either concentrated whey protein powder or whey protein isolate.

8. A method for preparing a composition for relieving physical fatigue, characterized in that, include: The composition is obtained by uniformly mixing 10-25 parts of soybean peptide, 20-50 parts of whey protein powder, 10-25 parts of sheep milk powder, 2-10 parts of leucine, 0.5-4 parts of acerola cherry powder, 0.1-2 parts of B-rich yeast, 2-8 parts of fructooligosaccharides, and 3-10 parts of jujube powder according to the weight ratio; wherein the whey protein powder, soybean peptide, and sheep milk powder are compounded in a ratio of 2:1:1, and the whey protein powder and leucine are added in a ratio of (3-8):

1.

9. The use of the composition according to any one of claims 1-7 in the preparation of food, medicine or health food for relieving physical fatigue.

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