Mild multi-effect anti-acne enzyme composition for teenagers and application thereof

Abstract

The invention provides a mild multi-effect anti-acne enzyme composition for teenagers and application of the mild multi-effect anti-acne enzyme composition. The composition comprises a fig extract, a roselle extract and keratinase. The composition provided by the invention is safe and non-irritant in components, the fig extract and the keratinase play a cutin renewal role of biological enzyme, and the roselle extract fundamentally promotes the generation of skin renewal related enzymes and assists in accelerating the decomposition of keratosuppository. The three components are synergistically compatible, pores are dredged, the skin problems such as teenager closed acne and blackhead are solved, and efficient and mild acne resistance is achieved.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to a mild, multi-effect anti-acne enzyme composition for adolescents and its application. Background Technology

[0002] Due to hormonal changes, adolescents in puberty are prone to increased sebum secretion and active keratinocyte proliferation, leading to abnormal keratinization of the hair follicle sebaceous gland duct. This results in keratin buildup clogging pores, causing frequent and recurring acne and other skin problems. Therefore, unclogging pores and promoting keratin renewal are crucial for adolescents' acne control.

[0003] CN118766783A discloses an acne-repairing composition containing collagen, comprising an acne-repairing component, a phosphate, and collagen; the acne-repairing component is selected from at least one of azelaic acid, salicylic acid, glycolic acid, lactobionic acid, mandelic acid, and lactic acid; the phosphate is selected from at least one of disodium hydrogen phosphate, sodium dihydrogen phosphate, dipotassium hydrogen phosphate, and potassium dihydrogen phosphate. This composition effectively balances acne-repairing efficacy with gentleness.

[0004] CN114569513A discloses an acne-removing and antibacterial skincare product, which comprises the following components by weight percentage: 25-40% active extract, 2-5% pine needle extract, 0.04-0.48% citric acid-modified mixture, 5-10% α-lipoic acid, 2-8% orange leaf essential oil, 0.6-1.2% papain, 5-10% moisturizer, 0.2-0.8% preservative, 1-6% acne-removing and antibacterial extract, and the balance being water. This skincare product has good whitening, brightening, and moisturizing effects, and can effectively penetrate the stratum corneum to act on the skin, fading acne scars and reducing skin inflammation.

[0005] Currently, the main exfoliating ingredients on the market include acids and enzymes. The most common acids are alpha-hydroxy acids (AHAs) and beta-hydroxy acids (Salicylic acid), which primarily work by dissolving the connections between keratinocytes to promote exfoliation. However, teenagers have thinner skin and less skincare experience, and improper use of acids can easily lead to problems such as a thinner stratum corneum, dryness, flaking, and a damaged skin barrier, turning their skin into sensitive skin. Enzymes break down keratin through biocatalytic reactions, exfoliating layer by layer and promoting skin renewal. Their effects are similar to acids, but the exfoliation process is controllable and relatively gentle. While common enzymes are gentle, they are slower to take effect and have weaker efficacy, making them insufficient to meet the needs of teenagers with frequent acne breakouts who require highly effective acne treatment.

[0006] Therefore, there is an urgent need to develop a mild and highly effective anti-acne enzyme composition suitable for adolescent skin, avoiding the irritation of acidic ingredients to the skin, and promoting keratin renewal more effectively and gently than traditional bio-enzymes, making it more suitable for adolescents with thin skin. Summary of the Invention

[0007] To address the shortcomings of existing technologies, the present invention aims to provide a mild and multi-effect anti-acne enzyme composition for adolescents and its application. The composition of the present invention is mild and non-irritating, can accelerate skin keratin renewal, effectively unclog pores, and improve skin problems such as closed comedones and blackheads in adolescents.

[0008] To achieve this objective, the present invention adopts the following technical solution:

[0009] In a first aspect, the present invention provides a mild and multi-effect anti-acne enzyme composition for adolescents, the composition comprising fig extract, hibiscus extract and keratinase.

[0010] Fig extract is rich in fig protease, a cysteine ​​protease, which is gentle, has low allergenicity, and high exfoliation efficiency. Furthermore, the natural active ingredients in fig extract (such as flavonoids, polyphenols, polysaccharides, and vitamins) synergistically work with fig protease to further reduce oxidative damage, soothe sensitive skin, promote skin barrier repair, and help inhibit bacteria. Roselle extract can promote skin renewal-related enzymatic reactions, accelerate cell metabolism, and improve keratin buildup from the root. Keratinase, a serine protease, can hydrolyze keratin, accelerate the breakdown of keratin plugs, and unclog pores.

[0011] The composition provided by this invention is safe and non-irritating. Fig extract and keratinase exert the keratin renewal effect of biological enzymes, while hibiscus extract promotes the production of skin renewal-related enzymes from the root, helping to accelerate the decomposition of keratin plugs. The three components work synergistically to unclog pores, improve skin problems such as closed comedones and blackheads in teenagers, and achieve highly effective and gentle anti-acne treatment.

[0012] Preferably, the composition comprises, by weight, 0.1-20 parts of fig extract, 0.1-5 parts of hibiscus extract, and 0.1-1 parts of keratinase.

[0013] The weight parts of the fig extract can be 0.1 parts, 0.2 parts, 0.5 parts, 0.7 parts, 1 part, 3 parts, 5 parts, 8 parts, 10 parts, 13 parts, 15 parts, 18 parts, or 20 parts, etc.

[0014] The weight parts of the hibiscus extract can be 0.1 parts, 0.2 parts, 0.5 parts, 0.7 parts, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, or 5 parts, etc.

[0015] The weight fraction of the keratinase can be 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9 or 1 part, etc.

[0016] Other specific point values ​​within the range of the above values ​​can be selected, and will not be elaborated on here.

[0017] Preferably, the composition further includes a thiol activator.

[0018] Preferably, the composition further comprises 0.1-2 parts by weight of a thiol activator.

[0019] The weight parts of the thiol activator can be 0.1 parts, 0.2 parts, 0.5 parts, 0.7 parts, 1 part, 1.3 parts, 1.5 parts, 1.8 parts, or 2 parts, etc.

[0020] Other specific point values ​​within the range of the above values ​​can be selected, and will not be elaborated on here.

[0021] Preferably, the thiol activator includes any one or a combination of at least two of glutathione, ergothioneine, or acetylcysteine.

[0022] In this invention, the thiol activator can open the disulfide bonds of skin keratin. The cleavage of the disulfide bonds changes the conformation of keratin, exposing more protease action sites, promoting the exfoliation effect of fig extract and keratinase, indirectly affecting the skin renewal enzymatic reaction of hibiscus extract, and enhancing the anti-acne efficacy of the enzyme composition.

[0023] Preferably, the thiol activator includes glutathione, ergothioneine, and acetylcysteine.

[0024] In this invention, glutathione, ergothioneine, and acetylcysteine ​​act as thiol activators and have a synergistic effect. The combination of the three can better accelerate the enzymatic reaction and enhance the anti-acne efficacy.

[0025] Preferably, the mass ratio of glutathione, ergothioneine and acetylcysteine ​​is (1-10):(1-10):(1-10).

[0026] The specific point values ​​in the first (1-10) can be 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10, etc.

[0027] The specific point values ​​in the second (1-10) can be 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10, etc.

[0028] The specific point values ​​in the third (1-10) can be 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10, etc.

[0029] Other specific point values ​​within the range of the above values ​​can be selected, and will not be elaborated on here.

[0030] Preferably, the composition further includes a reducing agent.

[0031] Preferably, the composition further comprises 0.1-2 parts by weight of reducing agent.

[0032] The reducing agent can be present in parts by weight of 0.1, 0.2, 0.5, 0.7, 1, 1.3, 1.5, 1.8, or 2 parts, etc.

[0033] Other specific point values ​​within the range of the above values ​​can be selected, and will not be elaborated on here.

[0034] Preferably, the reducing agent includes any one or a combination of at least two of sodium sulfite, thiotaurine, or sodium metabisulfite.

[0035] In this invention, the reducing agent can keep the enzyme active sites in keratinase and fig extract in a reduced state, improve enzyme activity, accelerate the metabolism and renewal of skin keratin, effectively remove dead skin cells, and have a better anti-acne effect.

[0036] In this invention, the thiol activator and reducing agent work synergistically to enhance the keratin renewal rate of the composition, thereby improving the problem of keratin buildup in adolescent skin from the root cause, allowing sebum to be discharged smoothly, and leaving the skin refreshed and clean.

[0037] In a second aspect, the present invention provides the use of the composition as described in the first aspect in the preparation of cosmetics.

[0038] Preferably, the cosmetic includes any one of toner, lotion, cream, serum, spray, mask, sunscreen, shampoo, or shower gel.

[0039] Preferably, the composition has a mass percentage content of 0.1-10% in the cosmetic, for example, it can be 0.1%, 0.2%, 0.5%, 0.7%, 1%, 3%, 5%, 8% or 10%, etc.

[0040] Other specific point values ​​within the range of the above values ​​can be selected, and will not be elaborated on here.

[0041] Preferably, the cosmetic also includes any one or a combination of at least two of the following: antioxidants, emollients, moisturizers, emulsifiers, thickeners, solubilizers, chelating agents, pH adjusters, solvents, preservatives, or fragrances.

[0042] Thirdly, the present invention provides a mild and multi-effect anti-acne lotion for teenagers, wherein the anti-acne lotion comprises, by weight percentage, 0.1-10% of the composition described in the first aspect, 0.1-3% of the efficacy adjuvant, 5-15% of the moisturizer, 0.1-1% of the antioxidant, 0.01-0.1% of the chelating agent, 0.05-0.4% of the thickener and 60-95% of the solvent.

[0043] The mass percentage of the composition can be 0.1%, 0.2%, 0.5%, 0.7%, 1%, 1.3%, 1.5%, 1.8%, 2%, 3%, 5%, 8%, or 10%, etc.

[0044] The mass percentage of the efficacy adjuvant can be 0.1%, 0.2%, 0.5%, 0.7%, 1%, 1.3%, 1.5%, 1.8%, 2%, 2.5%, or 3%, etc.

[0045] The mass percentage of the humectant can be 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, or 15%, etc.

[0046] The antioxidant content by mass percentage can be 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, or 1%, etc.

[0047] The mass percentage of the chelating agent can be 0.01%, 0.02%, 0.03%, 0.04%, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, or 0.1%, etc.

[0048] The mass percentage of the thickener can be 0.05%, 0.07%, 0.1%, 0.13%, 0.15%, 0.17%, 0.2%, 0.25%, 0.3%, 0.35%, or 0.4%, etc.

[0049] The mass percentage of the solvent can be 60%, 62%, 65%, 67%, 70%, 73%, 75%, 78%, 80%, 85%, 90%, or 95%, etc.

[0050] Other specific point values ​​within the range of the above values ​​can be selected, and will not be elaborated on here.

[0051] The anti-acne solution provided by this invention has safe and non-irritating ingredients. Fig extract and keratinase exert the keratin renewal effect of biological enzymes, while hibiscus extract promotes the production of skin renewal-related enzymes from the root, helping to accelerate the decomposition of keratin plugs. The three components work synergistically to unclog pores and improve skin problems such as closed comedones and blackheads in teenagers, achieving highly effective and gentle anti-acne treatment.

[0052] Preferably, the efficacy adjuvant includes any one or a combination of at least two of the following: rosewood seed extract, kudzu root extract, or pomegranate peel extract.

[0053] Preferably, the efficacy adjuvants include rosewood seed extract, kudzu root extract, and pomegranate peel extract.

[0054] In this invention, the extracts of redwood seeds, kudzu root, and pomegranate peel have a synergistic effect, which can synergistically enhance the anti-acne efficacy of the enzyme composition.

[0055] Preferably, the mass ratio of the rosewood seed extract, kudzu root extract and pomegranate peel extract is (1-10):(1-10):(1-10).

[0056] The specific point values ​​in the first (1-10) can be 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10, etc.

[0057] The specific point values ​​in the second (1-10) can be 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10, etc.

[0058] The specific point values ​​in the third (1-10) can be 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10, etc.

[0059] Other specific point values ​​within the range of the above values ​​can be selected, and will not be elaborated on here.

[0060] Preferably, the moisturizer comprises any one or a combination of at least two of butylene glycol, glycerin, 1,2-hexanediol, propylene glycol, 1,2-pentanediol, or sodium hyaluronate.

[0061] Preferably, the antioxidant comprises any one or a combination of at least two of p-hydroxyacetophenone, tocopheryl acetate, or retinyl palmitate.

[0062] Preferably, the chelating agent comprises disodium EDTA and / or tetrasodium EDTA.

[0063] Preferably, the thickener comprises any one or a combination of at least two of the following: ammonium acryloyldimethyl taurate / VP copolymer, acrylate / C10-30 alkanol acrylate crosspolymer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, carbomer, xanthan gum, sodium polyacrylate, or hydroxyethyl cellulose.

[0064] Preferably, the solvent comprises a polyol and / or water.

[0065] Preferably, the polyol includes any one or a combination of at least two of 1,2-hexanediol, 1,2-pentanediol, ethylene glycol, propylene glycol, butanediol, glycerol, polyethylene glycol, or sorbitol.

[0066] Preferably, the fig extract, hibiscus extract, rosewood seed extract, kudzu root extract, or pomegranate peel extract is prepared by a method comprising the following steps:

[0067] The plant materials and extraction reagents are mixed and subjected to high-pressure pulse treatment to separate the solid and liquid, and the liquid is collected to obtain the extract; the plant materials include any one of fig, roselle, redwood seed, kudzu root or pomegranate peel.

[0068] The preparation of plant extracts involved in this invention includes, but is not limited to, the methods described above. Extracts obtained from the aforementioned plants using methods commonly used in the art are all applicable to this invention. Furthermore, extracts prepared from the flowers, fruits, leaves, or roots of the aforementioned plants are all applicable to this invention and exert anti-acne effects in the formulations of this invention.

[0069] Preferably, the mass ratio of the plant material to the extraction reagent is 1:(5-15).

[0070] The specific point values ​​in (5-15) can be 5, 6, 7, 8, 9, 10, 11, 12, 13, 14 or 15, etc.

[0071] Other specific point values ​​within the range of the above values ​​can be selected, and will not be elaborated on here.

[0072] Preferably, the extraction reagent comprises a polyol and water.

[0073] Preferably, the polyol comprises any one or a combination of at least two of glycerol, 1,3-propanediol, 1,2-hexanediol, 1,2-pentanediol, 1,3-butanediol, ethanol, or methanol.

[0074] Preferably, the volume percentage of the polyol in the extraction reagent is 10-70%, for example, it can be 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 60% or 70%, etc.

[0075] Preferably, the electric field strength of the high-voltage pulse is 20-50kV / m, for example, it can be 20kV / m, 25kV / m, 30kV / m, 35kV / m, 40kV / m, 45kV / m or 50kV / m, etc.

[0076] Preferably, the number of pulses is 50-100, for example, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95 or 100.

[0077] Other specific point values ​​within the range of the above values ​​can be selected, and will not be elaborated on here.

[0078] Preferably, the collected liquid further includes a concentration and drying step.

[0079] Fourthly, the present invention provides a method for preparing the anti-acne solution as described in the third aspect, the method comprising:

[0080] (1) The chelating agent, thickener and solvent are mixed and homogenized to obtain phase A;

[0081] The humectant and antioxidant were mixed and homogenized, and then subjected to heating, heat preservation, and cooling in sequence to obtain phase B;

[0082] (2) Add phase B, the composition as described in the first aspect, and the efficacy adjuvant to phase A, homogenize, and obtain the anti-acne water.

[0083] Preferably, the homogenization method is stirring.

[0084] Preferably, the stirring speed is 50-60 rpm, for example, it can be 50 rpm, 51 rpm, 52 rpm, 53 rpm, 54 rpm, 55 rpm, 56 rpm, 57 rpm, 58 rpm, 59 rpm or 60 rpm, etc.

[0085] Preferably, the heating process includes raising the temperature to 80-85°C, for example, 80°C, 80.5°C, 81°C, 81.5°C, 82°C, 82.5°C, 83°C, 83.5°C, 84°C, 84.5°C, or 85°C.

[0086] Preferably, the heat preservation temperature is 80-85℃, for example, it can be 80℃, 80.5℃, 81℃, 81.5℃, 82℃, 82.5℃, 83℃, 83.5℃, 84℃, 84.5℃ or 85℃, etc.; the time is 8-12min, for example, it can be 8min, 8.5min, 9min, 9.5min, 10min, 10.5min, 11min, 11.5min or 12min, etc.

[0087] Preferably, the cooling process includes reducing the temperature to 20-50°C, for example, 20°C, 25°C, 30°C, 35°C, 40°C, 45°C, or 50°C.

[0088] Other specific point values ​​within the range of the above values ​​can be selected, and will not be elaborated on here.

[0089] Compared with the prior art, the present invention has the following beneficial effects:

[0090] The composition and anti-acne water ingredients provided by this invention are safe and non-irritating. Fig extract and keratinase exert the keratin renewal effect of biological enzymes, while hibiscus extract promotes the production of skin renewal-related enzymes from the root, helping to accelerate the decomposition of keratin plugs. The three components work synergistically to unclog pores and improve skin problems such as closed comedones and blackheads in teenagers, achieving highly effective and gentle anti-acne treatment. Detailed Implementation

[0091] To further illustrate the technical means and effects of the present invention, the following describes the technical solution of the present invention in conjunction with preferred embodiments of the present invention. However, the present invention is not limited to the scope of the embodiments.

[0092] Where specific techniques or conditions are not specified in the examples, they shall be performed in accordance with the techniques or conditions described in the literature in this field, or in accordance with the product instructions. Reagents or instruments whose manufacturers are not specified are all conventional products that can be purchased through legitimate channels.

[0093] The sources of materials used in the following specific embodiments are as follows:

[0094] Keratinase was purchased from ICBIO (Phyto-Keratinase); papain was purchased from Uniproma (Protesse G66); bromelain was purchased from Spec-Chem Industry (SpecPure BML120). Ammonium acryloyldimethyl taurate / VP copolymer was purchased from Clariant Chemicals (Aristoflex AVC); acrylate / C10-30 alkanol acrylate crosspolymer was purchased from Tinci Materials (SALTEX A10); hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer was purchased from SEPINOV EMT 10.

[0095] Preparation Example 1

[0096] This preparation example provides a fig extract, which is prepared by a method comprising the following steps:

[0097] Figs and 40% ethanol aqueous solution were mixed at a mass ratio of 1:10, and the mixture was subjected to high-voltage pulse treatment at an electric field strength of 35 kV / m for 80 cycles. The mixture was then filtered, the liquid was collected, concentrated, and dried to obtain fig extract.

[0098] Preparation Example 2

[0099] This preparation example provides a roselle extract, which is prepared by a method comprising the following steps:

[0100] Roselle and 70% methanol aqueous solution were mixed at a mass ratio of 1:5, and the mixture was subjected to high-voltage pulse treatment 100 times with an electric field strength of 20 kV / m. The mixture was then filtered, the liquid was collected, concentrated and dried to obtain roselle extract.

[0101] Preparation Example 3

[0102] This preparation example provides a rosewood seed extract, which is prepared by a method comprising the following steps:

[0103] Rosewood seeds and a 10% 1,3-propanediol aqueous solution were mixed at a mass ratio of 1:15. The mixture was subjected to a high-voltage pulse treatment with an electric field strength of 50 kV / m for 50 cycles. The mixture was then filtered, the liquid was collected, concentrated, and dried to obtain the rosewood seed extract.

[0104] Preparation Example 4

[0105] This preparation example provides a kudzu root extract, which differs from Preparation Example 1 only in that figs are replaced with kudzu root.

[0106] Preparation Example 5

[0107] This preparation example provides a pomegranate peel extract, which differs from Preparation Example 1 only in that figs are replaced with pomegranate peel.

[0108] Example 1

[0109] This embodiment provides an anti-acne enzyme composition, which comprises the following components in parts by weight:

[0110] The mixture consists of 10 parts of fig extract from Preparation Example 1, 4 parts of hibiscus extract from Preparation Example 2, 0.5 parts of keratinase, 0.3 parts of glutathione, 0.3 parts of ergothioneine, 0.4 parts of acetylcysteine, and 1 part of sodium sulfite. The preparation method involves simply mixing the above ingredients thoroughly.

[0111] Example 2

[0112] This embodiment provides an anti-acne enzyme composition, which comprises the following components in parts by weight:

[0113] The mixture consisted of 15 parts of fig extract from Preparation Example 1, 0.1 parts of hibiscus extract from Preparation Example 2, 0.1 parts of keratinase, 0.1 parts of glutathione, 0.2 parts of ergothioneine, 0.1 parts of acetylcysteine, and 0.1 parts of sodium sulfite. The preparation method involved simply mixing the above ingredients thoroughly.

[0114] Example 3

[0115] This embodiment provides an anti-acne enzyme composition, which comprises the following components in parts by weight:

[0116] The mixture consists of 5 parts fig extract from Preparation Example 1, 5 parts hibiscus extract from Preparation Example 2, 1 part keratinase, 0.7 parts glutathione, 0.6 parts ergothioneine, 0.7 parts acetylcysteine, and 2 parts sodium sulfite. The preparation method involves simply mixing the above ingredients thoroughly.

[0117] Example 4

[0118] This embodiment provides an anti-acne enzyme composition, which differs from Example 1 only in that: glutathione is not added, and its reduced amount is proportionally allocated to ergothioneine and acetylcysteine, while the remaining raw materials remain unchanged.

[0119] Example 5

[0120] This embodiment provides an anti-acne enzyme composition, which differs from Example 1 only in that: ergothioneine is not added, and its reduced amount is proportionally allocated to glutathione and acetylcysteine, while the remaining raw materials remain unchanged.

[0121] Example 6

[0122] This embodiment provides an anti-acne enzyme composition, which differs from Example 1 only in that: acetylcysteine ​​is not added, and its reduced amount is proportionally allocated to glutathione and ergothioneine, while the remaining raw materials remain unchanged.

[0123] Example 7

[0124] This embodiment provides an anti-acne enzyme composition, which differs from Example 1 only in that: glutathione, ergothioneine and acetylcysteine ​​are not added, their reduced amounts are allocated to sodium sulfite in proportion, and the remaining raw materials remain unchanged.

[0125] Example 8

[0126] This embodiment provides an anti-acne enzyme composition, which differs from Example 1 only in that: sodium sulfite is not added, and its reduced amount is proportionally allocated to glutathione, ergothioneine and acetylcysteine, while the remaining raw materials remain unchanged.

[0127] Example 9

[0128] This embodiment provides an anti-acne enzyme composition, which differs from Example 1 only in that sodium sulfite is replaced with an equal amount of thiotaurine, while the other raw materials remain unchanged.

[0129] Comparative Example 1

[0130] This comparative example provides an anti-acne enzyme composition that differs from Example 1 only in that: fig extract is not added, and its reduced amount is allocated proportionally to hibiscus extract and keratinase, while the remaining ingredients remain unchanged.

[0131] Comparative Example 2

[0132] This comparative example provides an anti-acne enzyme composition that differs from Example 1 only in that: hibiscus extract is not added, its reduced amount is allocated proportionally to fig extract and keratinase, and the remaining ingredients remain unchanged.

[0133] Comparative Example 3

[0134] This comparative example provides an anti-acne enzyme composition that differs from Example 1 only in that: no keratinase is added, and its reduced amount is allocated proportionally to fig extract and hibiscus extract, while the remaining ingredients remain unchanged.

[0135] Comparative Example 4

[0136] This comparative example provides an anti-acne enzyme composition, which differs from Example 1 only in that keratinase is replaced with an equal amount of papain, while all other ingredients remain unchanged.

[0137] Comparative Example 5

[0138] This comparative example provides an anti-acne enzyme composition, which differs from Example 1 only in that keratinase is replaced with an equal amount of bromelain, while all other ingredients remain unchanged.

[0139] Application Example 1

[0140] This application example provides an anti-acne solution, which comprises the following components by weight percentage:

[0141] Table 1

[0142]

[0143] Its preparation method is as follows:

[0144] (1) The chelating agent, thickener and solvent are mixed and stirred evenly at 55 rpm to obtain phase A; the humectant and antioxidant are mixed and stirred evenly at 50 rpm, first heated to 82℃, held for 10 min, and then cooled to 35℃ to obtain phase B;

[0145] (2) Add phase B, anti-acne enzyme composition and efficacy adjuvant to phase A, and stir evenly at 60 rpm to obtain the anti-acne water.

[0146] Application Example 2

[0147] This application example provides an anti-acne solution, which comprises the following components by weight percentage:

[0148] Table 2

[0149]

[0150] Its preparation method is as follows:

[0151] (1) The chelating agent, thickener and solvent are mixed and stirred evenly at 50 rpm to obtain phase A; the humectant and antioxidant are mixed and stirred evenly at 60 rpm, first heated to 80℃, held for 12 min, and then cooled to 20℃ to obtain phase B;

[0152] (2) Add phase B, anti-acne enzyme composition and efficacy adjuvant to phase A, and stir evenly at 55 rpm to obtain the anti-acne water.

[0153] Application Example 3

[0154] This application example provides an anti-acne solution, which comprises the following components by weight percentage:

[0155] Table 3

[0156]

[0157] Its preparation method is as follows:

[0158] (1) The chelating agent, thickener and solvent are mixed and stirred evenly at 60 rpm to obtain phase A; the humectant and antioxidant are mixed and stirred evenly at 55 rpm, first heated to 85℃, held for 8 min, and then cooled to 50℃ to obtain phase B;

[0159] (2) Add phase B, anti-acne enzyme composition and efficacy adjuvant to phase A, and stir evenly at 50 rpm to obtain the anti-acne water.

[0160] Application Example 4-9

[0161] This application example provides an anti-acne solution, which differs from application example 1 only in that the anti-acne enzyme composition provided in example 1 is replaced with an equal amount of the anti-acne enzyme composition provided in examples 4-9, while all other raw materials remain unchanged.

[0162] Application Example 10

[0163] This application example provides an anti-acne lotion, which differs from Application Example 1 only in that: no redwood seed extract is added, and its reduced amount is proportionally allocated to kudzu root extract and pomegranate peel extract, while the remaining ingredients remain unchanged.

[0164] Application Example 11

[0165] This application example provides an anti-acne lotion, which differs from Application Example 1 only in that: kudzu root extract is not added, and its reduced amount is proportionally allocated to redwood seed extract and pomegranate peel extract, while the remaining ingredients remain unchanged.

[0166] Application Example 12

[0167] This application example provides an anti-acne lotion, which differs from Application Example 1 only in that: pomegranate peel extract is not added, and its reduced amount is proportionally allocated to redwood seed extract and kudzu root extract, while the remaining ingredients remain unchanged.

[0168] Compare and contrast examples 1-5

[0169] This comparative application example provides an anti-acne solution, which differs from application example 1 only in that the anti-acne enzyme composition provided in example 1 is replaced with an equal amount of the anti-acne enzyme composition provided in comparative examples 1-5, while all other raw materials remain unchanged.

[0170] Test Example 1

[0171] Add 1g of the anti-acne enzyme composition provided in Examples 1-9 or Comparative Examples 1-3 to a test tube, followed by 1mL of 0.035% (w / v) EDTA solution. Mix well and incubate at 37°C for 10min. Then add 2mL of 1.0% (w / v) casein solution, mix immediately, and incubate at 37°C for 20min. After the reaction is complete, add 3mL of 6.5% (w / v) trichloroacetic acid solution, mix well, and let stand for 10min. Then centrifuge at 10000rpm for 5min, collect the supernatant in a new test tube, and measure the absorbance at 275nm. All solutions were prepared using PBS buffer as the solvent. Higher absorbance indicates stronger enzyme activity and better hydrolysis of keratin plugs.

[0172] The test results are shown in Table 4. The synergistic combination of fig extract, hibiscus extract, and keratinase in this invention exhibits high enzyme activity, accelerating the decomposition of keratin plugs and effectively removing dead skin cells. The thiol activator exposes more protease action sites, while the reducing agent maintains the enzyme activity sites in a highly reactive state, accelerating skin cell metabolism and renewal, and improving skin problems in adolescents.

[0173] Table 4

[0174] Test sample OD value Example 1 2.389 Example 2 2.471 Example 3 2.286 Example 4 2.229 Example 5 2.010 Example 6 1.988 Example 7 1.426 Example 8 1.922 Example 9 1.956 Comparative Example 1 1.026 Comparative Example 2 1.335 Comparative Example 3 1.242

[0175] Test Example 2

[0176] The subjects were 180 healthy men or women aged 18-40, divided into 10 groups. 0.1 mL of 10% (w / v) dihydroxyacetone was applied to the inside of the subjects' arms (lower and upper arms) on a 1.5×1.5 cm gauze pad. After 24 hours, the gauze was removed. 48 hours later, the skin was discolored to brown. The ITA° value of the discolored area was measured using a colorimeter. The anti-acne lotion provided in Examples 1-12 or Comparative Examples 1-5 was evenly applied to the discolored area twice daily, morning and evening, for 10 consecutive days. The ITA° value of the discolored area was measured again using a colorimeter. A blank control group was established where no anti-acne lotion or any skincare products were applied. The higher the ITA° value, the lighter the skin tone; the lower the ITA° value, the darker the skin tone. Calculate the rate of change of ITA° value before and after using the anti-acne lotion to evaluate its effect on promoting skin cell turnover. ITA° value change rate (%) = ((ITA° value after use - ITA° value before use) / ITA° value before use) × 100. The higher the rate of change of ITA° value, the faster the skin cell turnover.

[0177] The test results are shown in Table 5. In this invention, fig extract, hibiscus extract, and keratinase synergistically accelerate skin keratin renewal, improve the problem of skin keratin buildup, unclog pores, and allow sebum to be discharged smoothly, leaving the skin clean and refreshed. Thiol activators and reducing agents assist in enhancing the efficiency of the enzymatic reaction, accelerating the metabolism and renewal of skin keratin. Rosewood seed extract, kudzu root extract, and pomegranate peel extract have a synergistic effect, synergistically increasing the keratin renewal rate of the enzyme composition.

[0178] Table 5

[0179] Test sample ITA° value change rate (%) Application Example 1 198.65 Application Example 2 186.43 Application Example 3 216.58 Application Example 4 169.44 Application Example 5 172.35 Application Example 6 162.76 Application Example 7 146.96 Application Example 8 155.38 Application Example 9 160.64 Application Example 10 162.58 Application Example 11 157.82 Application Example 12 164.57 Comparative Application Example 1 126.35 Comparative Application Example 2 129.71 Comparative Application Example 3 133.85 Comparative Application Example 4 136.92 Comparative Application Example 5 122.76 Blank control 86.84

[0180] Test Example 3

[0181] The subjects were 180 healthy men or women aged 18-40, divided into 10 groups. All participants had a certain number of acne spots on their facial skin, but these could not be nodules or cysts that were difficult to improve with clinical formulations. The participants used the acne-fighting lotion provided in Examples 1-12 or the control examples 1-5, twice daily, morning and evening, without using any other cosmetics. A blank control group was formed where no acne-fighting lotion or any skincare products were applied. Before use and after 10 days of continuous use, laboratory researchers counted the acne spots on the participants' faces and calculated the change in acne number: change in acne number = number of acne spots after use - number of acne spots before use. Skin images were taken using a standard image capture device, and the red area α-value under polarized light was analyzed. A smaller α-value indicates milder acne inflammation. The α-value change rate was calculated as: α-value change rate (%) = ((α-value after use - α-value before use) / α-value before use) × 100. A smaller α-value change rate indicates better acne treatment effect.

[0182] The test results are shown in Table 6. In this invention, fig extract, hibiscus extract, and keratinase synergistically improve the problem of skin keratin buildup, unclog pores, and have excellent acne-removing effects. Thiol activators and reducing agents assist in improving the efficiency of the enzymatic reaction, accelerating the metabolism and renewal of skin keratin, and enhancing the acne-removing effect. Rosewood seed extract, kudzu root extract, and pomegranate peel extract have a synergistic effect, synergistically increasing the keratin renewal rate of the enzyme composition and enhancing the acne-removing effect.

[0183] Table 6

[0184] Test sample Change in the number of pimples Rate of change of value a (%) Application Example 1 -9 -21.34 Application Example 2 -8 -19.46 Application Example 3 -11 -21.68 Application Example 4 -6 -16.76 Application Example 5 -7 -16.88 Application Example 6 -6 -15.43 Application Example 7 -4 -12.75 Application Example 8 -5 -13.61 Application Example 9 -5 -15.48 Application Example 10 -6 -16.38 Application Example 11 -6 -15.62 Application Example 12 -7 -16.85 Comparative Application Example 1 -3 -8.46 Comparative Application Example 2 -3 -8.79 Comparative Application Example 3 -2 -7.57 Comparative Application Example 4 -3 -9.15 Comparative Application Example 5 -2 -7.22 Blank control -1 -4.63

[0185] Test Example 4

[0186] Take chicken embryos at 10 days of gestation, mark the location of the air cell, peel off the eggshell, add 0.9% (w / v) sodium chloride solution to completely moisten the eggshell membrane, and carefully peel off the eggshell membrane to expose the vascular membrane rich in capillaries, i.e., CAM. After photographing and recording the initial state of the CAM, cover a polytetrafluoroethylene resin ring with a layer of flat plastic wrap; use a pipette to transfer 40 μL of the anti-acne solution provided in Examples 1-3, spread it evenly on the ring, and then cover it on the CAM membrane, allowing the test substance to directly contact the CAM. Then, place the chicken embryo in an environment with a temperature of 37°C and a relative humidity of 60%. After 30 minutes, remove the test chicken embryo, remove the resin ring, photograph and record the test area, and observe the degree of vascular damage under a microscope. Score according to the degree of vascular damage. Each sample is tested in triplicate, and the result is the average of all scores.

[0187] The evaluation criteria are as follows: (1) No reaction is observed, CAM is normal, and the vascular effect is no reaction, at which point the score is 0; (2) No blood flow in the blood vessels, the blood vessels are clear, and the vascular effect is ghost vessels, at which point the score is 1; (3) Congestion, or increased blood flow in the small blood vessels of CAM, and the vascular effect is capillary damage, at which point the score is 2; (4) There are 1-10 countable small bleeding points in the ring, and the vascular effect is very slight bleeding, at which point the score is 3; (5) There are more than 10 countable small bleeding points in the ring, or the small bleeding points are uncountable and cover less than 1 / 8 of the area in the ring, and the vascular effect is slight bleeding, at which point the score is 4. (6) The area covered by bleeding points in the ring is 1 / 8-1 / 4 or there are no bleeding points in the ring but the area covered by redness is less than 1 / 4, and the vascular effect is very mild bleeding, and the score is 5; (7) The area covered by bleeding points in the ring is 1 / 4-1 / 2 or there are no bleeding points in the ring but the area covered by redness is 1 / 4-1 / 2, and the vascular effect is mild bleeding, and the score is 6; (8) There are a large number of bleeding points in the ring or the bleeding range in a small area reaches 1 / 2-3 / 4, and the vascular effect is moderate bleeding, and the score is 7; (9) The area of ​​bleeding points in the ring is >3 / 4, and scabs may form, and the vascular effect is severe bleeding, and the score is 8.

[0188] Test results show that the anti-acne solution provided by this invention scored 0-1 in the cosmetic eye irritation test for chicken embryo chorioallantoic membrane vessels, indicating that it is non-irritating, has safe ingredients, and is gentle on acne, making it more suitable for teenagers with thin skin.

[0189] This invention illustrates a mild, multi-effect anti-acne enzyme composition for adolescents and its application through the above embodiments. However, this invention is not limited to the above embodiments, meaning that this invention does not necessarily rely on the above embodiments for implementation. Those skilled in the art should understand that any improvements to this invention, equivalent substitutions of the raw materials in the product, addition of auxiliary components, and selection of specific methods, all fall within the protection and disclosure scope of this invention.

[0190] The preferred embodiments of the present invention have been described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the scope of the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the protection scope of the present invention.

[0191] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not describe the various possible combinations separately.

Claims

1. A mild multi-purpose anti-acne enzyme composition for teenagers, characterized in that, The composition comprises fig extract, roselle extract and keratinase.

2. The composition of claim 1, wherein, The composition comprises, in parts by weight, 0.1-20 parts of fig extract, 0.1-5 parts of roselle extract and 0.1-1 part of keratinase.

3. The composition of claim 1, wherein, The composition further comprises a sulfhydryl activator; the sulfhydryl activator comprises any one or a combination of at least two of glutathione, ergothioneine or acetylcysteine; The composition further comprises a reducing agent; the reducing agent comprises any one or a combination of at least two of sodium sulfite, taurine or sodium metabisulfite.

4. The composition of claim 3, wherein, The composition further comprises, in parts by weight, 0.1-2 parts of sulfhydryl activator; the composition further comprises, in parts by weight, 0.1-2 parts of reducing agent; The sulfhydryl activator comprises glutathione, ergothioneine and acetylcysteine.

5. Use of the composition according to any one of claims 1-4 in the preparation of a cosmetic product.

6. Use according to claim 5, characterized in that, The cosmetic product comprises any one or a combination of at least two of cosmetic water, emulsion, cream, essence, spray, mask, sunscreen, shampoo or body wash; The composition is present in the cosmetic product in an amount of 0.1-10% by mass; The cosmetic product further comprises any one or a combination of at least two of antioxidant, emollient, humectant, emulsifier, thickener, solubilizer, chelating agent, pH adjuster, solvent, preservative or fragrance.

7. A mild multi- effect anti-acne water for teenagers, characterized in that, The anti-acne water comprises, in percentage by mass, 0.1-10% of the composition according to any one of claims 1-4, 0.1-3% of efficacy aid, 5-15% of humectant, 0.1-1% of antioxidant, 0.01-0.1% of chelating agent, 0.05-0.4% of thickener and 60-95% of solvent.

8. The anti-acne water of claim 7, wherein The efficacy aid comprises any one or a combination of at least two of Pterocarpus marsupium extract, Pueraria mirifica extract or Punica granatum peel extract; The humectant comprises any one or a combination of at least two of butylene glycol, glycerin, 1,2-hexanediol, propylene glycol, 1,2-pentanediol or sodium hyaluronate; The antioxidant comprises any one or a combination of at least two of p-hydroxyacetophenone, tocopheryl acetate or retinol palmitate; The chelating agent comprises disodium EDTA and / or tetrasodium EDTA; The thickener comprises any one or a combination of at least two of acryloyldimethyltaurine ammonium / VP copolymer, acrylate / C10-30 alkyl acrylate crosspolymer, hydroxyethyl acrylate / sodium acryloyldimethyltaurate copolymer, carbomer, xanthan gum, sodium polyacrylate or hydroxyethyl cellulose; The solvent comprises polyhydric alcohol and / or water; The polyhydric alcohol comprises any one or a combination of at least two of 1,2-hexanediol, 1,2-pentanediol, ethylene glycol, propylene glycol, butylene glycol, glycerin, polyethylene glycol or sorbitol.

9. The method of preparing an anti-acne water according to claim 7 or 8, characterized in that, The method comprises: (1) mixing homogeneously the chelating agent, thickener and solvent to obtain phase A; mixing homogeneously the humectant and antioxidant, and sequentially subjecting to heating, holding and cooling to obtain phase B; (2) adding phase B, the composition according to any one of claims 1-4 and the efficacy aid to phase A, and homogenizing to obtain the anti-acne water.

10. The method of claim 9, wherein, The homogenization is by stirring. The stirring speed is 50-60 rpm; The heating process includes increasing the temperature to 80-85℃; The holding temperature is 80-85℃, and the holding time is 8-12 min; The cooling process includes decreasing the temperature to 20-50℃.

Images