Anti-aging preparation with combination of human exosome and plant exosome as well as preparation method and application of anti-aging preparation

Abstract

The invention relates to the technical field of anti-aging preparations, and particularly discloses an anti-aging preparation of a human exosome combined with a plant exosome as well as a preparation method and application of the anti-aging preparation. The invention provides an anti-aging preparation combining a human exosome and a plant exosome. The anti-aging preparation specifically comprises the following components in parts by weight: 8-12 parts of the human exosome, 3-7 parts of the plant exosome and 0.5-1.5 parts of collagen, the preparation method of the plant exosome comprises the following steps: taking beet root tubers and mesembryanthemum crystallinum in a weight ratio of (0.5-3): (7-11) as raw materials, crushing the raw materials, adding a buffer solution, and then sequentially carrying out wall breaking treatment, gradient centrifugation to take supernate, dilution, deep filtration, concentration and purification to obtain the plant exosome. According to the technical scheme provided by the invention, the prepared anti-aging preparation has relatively strong oxidation resistance, saccharification resistance and stability, and has a relatively strong anti-aging effect when being used in skin care products.

Description

Technical Field

[0001] This application relates to the technical field of anti-aging agents, specifically to an anti-aging agent combining human exosomes and plant exosomes, its preparation method, and its application. Background Technology

[0002] Research on anti-aging agents is of profound necessity and urgency. Anti-aging agents can help people maintain physiological functions, cognitive abilities, and independent living abilities for a longer period of time in old age, reduce the duration of disability and dementia caused by age-related diseases, and thus significantly alleviate individual suffering, family care burden, and social medical expenditures.

[0003] Current anti-aging agents mainly rely on single-component drugs, but their efficacy is generally limited, with low antioxidant and anti-inflammatory effects, resulting in limited therapeutic effects. Summary of the Invention

[0004] To address the aforementioned technical problems, this application provides an anti-aging preparation of human exosomes combined with plant exosomes, its preparation method, and its application.

[0005] In one aspect, this application provides an anti-aging preparation combining human exosomes and plant exosomes, specifically comprising the following components in parts by weight: 8-12 parts human exosomes, 3-7 parts plant exosomes, and 0.5-1.5 parts collagen; The method for preparing plant exosomes is as follows: using beet root and ice plant in a weight ratio of 0.5-3:7-11 as raw materials, crush them and add buffer solution, then successively perform cell wall disruption treatment, gradient centrifugation to obtain supernatant, dilution and deep filtration, concentration and purification to obtain the product.

[0006] Preferably, the anti-aging preparation specifically comprises the following components in parts by weight: 9-11 parts human exosomes, 4-6 parts plant exosomes, and 0.7-1.2 parts collagen.

[0007] Using the technical solution provided in this application, human exosomes, plant exosomes, and collagen are used as raw materials, and the amount of each combined raw material is controlled so that the raw materials work together and have a synergistic effect. The resulting anti-aging preparation has strong antioxidant properties, anti-glycation properties, and stability; and it has a strong anti-aging effect when used in skin care products.

[0008] Human exosomes are rich in bioactive substances from their cells, including mRNA and proteins (such as enzymes, cytokines, and growth factors). These carried growth factors and signaling molecules have good antioxidant and anti-glycation effects. Specific miRNAs and proteins carried by exosomes can upregulate the skin cells' own antioxidant pathways and enhance the activity of intracellular antioxidant enzymes, thereby effectively reducing oxidative stress damage and protecting cell membranes, proteins, and DNA. Human exosomes can also regulate immune responses, reduce chronic low-grade inflammation of the skin, and help maintain a healthy skin microenvironment.

[0009] Beetroot exosomes, rich in betaine, various phenolic compounds, and vitamin C / E precursors, possess a strong ability to directly scavenge free radicals. They can serve as exogenous antioxidants and effectively capture glycation intermediates, combating free radical and glycation damage. Ice plant exosomes are rich in unique osmotic protectants (such as inositol and proline), flavonoids, polyphenols, and plant polysaccharides, exhibiting excellent antioxidant capabilities. Their abundant osmotic protectants help maintain cellular osmotic pressure balance, protect cell structure, and possess powerful water-locking and moisturizing properties. As natural nanovesicles, plant exosomes synergistically enhance the endogenous system activated by human exosomes, demonstrating excellent moisturizing and protein stability protection capabilities. They can more effectively penetrate the skin barrier, especially the stratum corneum, to deliver these abundant bioactive molecules to the deeper layers of the skin to exert their effects.

[0010] Collagen's key structural support and barrier repair can form a breathable film on the skin surface, reducing transepidermal water loss and providing immediate and significant moisturizing effects.

[0011] Therefore, the anti-aging formulation prepared in this application utilizes human exosomes to simulate and enhance intercellular regenerative signal transduction, plant exosomes (beetroot and ice plant) to provide powerful exogenous antioxidant, anti-glycation, and bioactive substance delivery, and collagen for barrier repair, immediate hydration, and auxiliary signal transduction. These three core ingredients work synergistically and mutually promote each other along three key anti-aging pathways: clearing damaging factors, actively repairing and rebuilding, and strengthening the defense barrier, thereby achieving significant anti-aging effects.

[0012] Preferably, the preparation method of the human exosomes is as follows: human umbilical cord mesenchymal stem cells are cultured in DMEM / F12 medium containing 10% fetal bovine serum and grown to the logarithmic growth phase; the human umbilical cord mesenchymal stem cells grown to the logarithmic growth phase are seeded in DMEM / F12 medium containing poloxamer 407 at a concentration of 3-10 ng / mL and 10% fetal bovine serum and cultured for proliferation for 48-72 h to obtain proliferated human umbilical cord mesenchymal stem cells; the original medium is aspirated, and then seeded in serum-free DMEM / F12 medium and cultured for 48-72 h, and the cell culture supernatant is collected; the cell culture supernatant is subjected to gradient centrifugation to obtain human umbilical cord mesenchymal stem cell exosomes.

[0013] In one specific implementation plan, during the proliferation culture process, the concentration of poloxamer 407 in the culture medium can be 3 ng / mL, 4 ng / mL, 5 ng / mL, 7 ng / mL, or 10 ng / mL.

[0014] Experimental analysis shows that, in the preparation method of human exosomes, this application selects to seed human umbilical cord mesenchymal stem cells grown to the logarithmic phase in DMEM / F12 medium containing poloxamer 407 at a concentration of 3-10 ng / mL and 10% fetal bovine serum for proliferation culture, which can further improve the performance of anti-aging agents.

[0015] Preferably, the specific steps of the gradient centrifugation are as follows: the cell culture supernatant is centrifuged once at 300-500g for 8-12 min to remove dead cells; then the supernatant after the first centrifugation is centrifuged a second time at 3000-5000g for 10-20 min to remove cell debris; then the supernatant after the second centrifugation is centrifuged a third time at 8000-12000g for 20-30 min to remove larger cell debris vesicles and apoptotic bodies; then the supernatant after the third centrifugation is centrifuged a fourth time at 60000-100000g for 40-60 min, the precipitate is collected, and the crude exosomes obtained are washed with PBS buffer and centrifuged a fifth time at 60000-100000g for 40-60 min, the precipitate is collected, and the purified human umbilical cord mesenchymal stem cell exosomes are obtained.

[0016] Preferably, the method for preparing the plant exosomes is as follows: using beetroot and ice plant in a weight ratio of 1-2:8-10 as raw materials, crushing them and adding buffer solution, then sequentially undergoing cell wall disruption treatment, gradient centrifugation to obtain the supernatant, dilution and deep filtration, and concentration and purification to obtain the product.

[0017] In one specific implementation method, the weight ratio of beetroot and ice plant in the preparation method of plant exosomes can be 0.5:7, 0.5:8, 0.5:9, 0.5:10, 0.5:11, 1:7, 1:8, 1:9, 1:10, 1:11, 1.5:7, 1.5:8, 1.5:9, 1.5:10, 1.5:11, 2:7, 2:8, 2:9, 2:10, 2:11, 3:7, 3:8, 3:9, 3:10, or 3:11.

[0018] Experimental analysis shows that, in the preparation method of plant exosomes, the use of beetroot and ice plant in the above weight ratio as raw materials can further improve the performance of anti-aging agents.

[0019] Preferably, the preparation method of the plant exosomes is as follows: using beet tubers and ice plant as raw materials, after grinding, PBS buffer is added at a solid-liquid ratio of 1:80-120, and then the cells are subjected to cell wall disruption treatment; the cells are centrifuged at 7000-10000 rpm for 40-80 min, and the supernatant is collected; the supernatant is diluted with 10-15 times its weight of PBS buffer, and then subjected to deep filtration using a tangential flow deep filtration membrane to concentrate and purify the product.

[0020] Preferably, the collagen is selected from one or more of rose collagen, bovine collagen peptides, and fish collagen tripeptides. Secondly, this application provides a method for preparing the above-mentioned anti-aging agent, the specific steps of which are: weighing the corresponding weights of human exosomes, plant exosomes and collagen respectively, mixing them evenly, and thus obtaining the anti-aging agent.

[0021] Thirdly, this application provides the application of the above-mentioned anti-aging agents in the preparation of anti-aging skin care products.

[0022] Fourthly, this application provides an anti-aging skincare product, including the aforementioned anti-aging preparation; the anti-aging skincare product comprises the following components in parts by weight: 50-60 parts water, 3-10 parts glycerin, 0.5-2 parts VC ethyl ether, and 0.5-1.5 parts anti-aging preparation.

[0023] In summary, the technical solution of this application has the following effects: Using the technical solution provided in this application, human exosomes, plant exosomes, and collagen are used as raw materials, and the amount of each combined raw material is controlled so that the raw materials work together and have a synergistic effect. The resulting anti-aging preparation has strong antioxidant and anti-glycation properties, and excellent stability.

[0024] The anti-aging preparation prepared in this application is used to prepare anti-aging skin care products, which can significantly increase the skin's moisture content, reduce skin wrinkles, and thus have a strong anti-aging effect. Detailed Implementation

[0025] The present application will be further described in detail below with reference to embodiments, comparative examples and performance test results. These embodiments should not be construed as limiting the scope of protection claimed in this application. Example

[0026] Examples 1-5 Examples 1-5 provide an anti-aging formulation combining human exosomes and plant exosomes, and their preparation methods.

[0027] The difference in the above embodiments is that the dosage of each component in the anti-aging preparation is different, as shown in Table 1.

[0028] The specific preparation method of the anti-aging agent in the above embodiments is shown below.

[0029] (1) Preparation of human umbilical cord mesenchymal stem cell exosomes Human umbilical cord mesenchymal stem cells (HMCs) were cultured in DMEM / F12 medium containing 10% fetal bovine serum (FBS) and incubated at 37°C in a 5% CO2 incubator until they reached the logarithmic growth phase. The HMCs in the logarithmic growth phase were then seeded into DMEM / F12 medium containing 5 ng / mL poloxamer 407 and 10% FBS and cultured at 37°C in a 5% CO2 incubator for 48 h to obtain proliferated HMCs. The original medium was removed, and the HMCs were then seeded into serum-free DMEM / F12 medium and cultured for 72 h. The cell culture supernatant was collected and subjected to gradient centrifugation to obtain HMC exosomes.

[0030] The specific steps of gradient centrifugation are as follows: At 4°C, the cell culture supernatant is centrifuged once at 400g for 10 min to remove dead cells; then, the supernatant after the first centrifugation is centrifuged a second time at 4000g for 15 min to remove cell debris; then, the supernatant after the second centrifugation is centrifuged a third time at 9000g for 25 min to remove larger cell debris vesicles and apoptotic bodies; then, the supernatant after the third centrifugation is centrifuged a fourth time at 80000g for 50 min, and the precipitate is collected. The crude exosomes obtained are washed with PBS buffer and then centrifuged a fifth time at 80000g for 50 min, and the precipitate is collected to obtain purified human umbilical cord mesenchymal stem cell exosomes.

[0031] (2) Preparation of plant exosomes Fresh beetroots and fresh ice plant were washed three times with purified water. The beetroots and ice plant were then mixed at a weight ratio of 1.5:9 and pulverized to a particle size ≤1mm. The pulverized material was transferred to a high-speed blender, and PBS buffer was added at a solid-liquid ratio of 1:100 for cell disruption. Gradient centrifugation was performed (the specific steps of gradient centrifugation are: centrifuge the cell culture supernatant at 400g for 5 min at 4℃; then centrifuge the supernatant at 2000g for 10 min; then centrifuge the supernatant at 6000g for 20 min; then centrifuge the supernatant at 10000g for 30 min, and collect the supernatant). The supernatant was diluted with 12 times its weight of PBS buffer, tangentially filtered through a depth filter membrane, concentrated, and purified. The resulting suspension is the plant exosome.

[0032] (3) Preparation of anti-aging agents According to Table 1, weigh out the corresponding weights of human exosomes, plant exosomes, and collagen (rose collagen, purchased from Xinyang Mufan Biotechnology Co., Ltd.), mix them evenly, and the anti-aging preparation is obtained.

[0033] Table 1. Dosage of each component in the anti-aging preparations of Examples 1-5 and Comparative Examples 1-2 Examples 6-9 Examples 6-9 provide an anti-aging formulation combining human exosomes and plant exosomes, and their preparation methods.

[0034] The difference between the above embodiments and Embodiment 1 is that the preparation methods of human exosomes are different, as detailed below.

[0035] In Example 6: DMEM / F12 medium containing 10% fetal bovine serum was used instead of DMEM / F12 medium containing 5 ng / mL poloxamer 407 and 10% fetal bovine serum.

[0036] In Example 7: DMEM / F12 medium containing 5 ng / mL poloxamer 188 and 10% fetal bovine serum was used instead of DMEM / F12 medium containing 5 ng / mL poloxamer 407 and 10% fetal bovine serum.

[0037] In Example 8: DMEM / F12 medium containing 5 ng / mL poloxamer 407 and 10% fetal bovine serum was used instead of DMEM / F12 medium containing 5 ng / mL poloxamer 407 and 10% fetal bovine serum.

[0038] In Example 9, DMEM / F12 medium containing 5 ng / mL poloxamer 407 and 10% fetal bovine serum was used instead of DMEM / F12 medium containing 5 ng / mL poloxamer 407 and 10% fetal bovine serum.

[0039] All other process parameters in the above embodiments are the same as those in Embodiment 1.

[0040] Examples 10-11 Examples 10-11 provide an anti-aging formulation combining human exosomes and plant exosomes, and their preparation methods.

[0041] The difference between the above embodiments and Embodiment 1 is that the preparation methods of plant exosomes are different, as detailed below.

[0042] In Example 10: In the method for preparing plant exosomes, the weight ratio of beetroot and ice plant is 3:7.

[0043] In Example 11: In the method for preparing plant exosomes, the weight ratio of beetroot and ice plant is 1:10.

[0044] All other process parameters in the above embodiments are the same as those in Embodiment 1.

[0045] Comparative Example Comparative Examples 1-2 Comparative Examples 1 and 2 respectively provide an anti-aging preparation of human exosomes combined with plant exosomes and its preparation method.

[0046] The difference between the above comparative example and Example 1 is that the amount of each component in the anti-aging preparation is different, as shown in Table 1.

[0047] All other process parameters in the above comparative examples are the same as those in Example 1.

[0048] Comparative Examples 3-5 Comparative Examples 3-5 provide an anti-aging formulation combining human exosomes and plant exosomes, and their preparation methods, respectively.

[0049] The differences between the above comparative examples and the embodiments are as follows.

[0050] In Comparative Example 3: In the method for preparing plant exosomes, an equal amount of white peony root was used to replace beetroot.

[0051] In Comparative Example 4: In the method for preparing plant exosomes, an equal amount of aloe vera was used to replace ice plant.

[0052] In Comparative Example 5: In the method for preparing plant exosomes: the weight ratio of beet root and ice plant was 9:1.5.

[0053] All other process parameters in the above comparative examples are the same as those in Example 1.

[0054] Performance testing (1) Antioxidant properties of anti-aging agents The antioxidant properties of anti-aging agents are evaluated by detecting the scavenging rate of DPPH free radicals by the anti-aging agents prepared in the examples or comparative examples.

[0055] The specific testing methods are as follows: The anti-aging agents prepared in the examples or comparative examples were prepared with deionized water to form a sample solution with a concentration of 0.1 g / L. 1 mL of the sample solution was placed in a test tube, and 1 mL of 0.2 mmol / L DPPH free radical anhydrous ethanol solution was added. After mixing, the mixture was reacted at room temperature in the dark for 30 min, and the absorbance value was measured at 517 nm.

[0056] Use 1 mL of deionized water instead of 1 mL of sample solution as the control group; use 1 mL of anhydrous ethanol instead of 1 mL of 0.2 mmol / L DPPH free radical solution as the blank group.

[0057] The DPPH radical scavenging rate is calculated using the following formula: DPPH radical scavenging rate (%) = [1 - (sample absorbance value - blank group absorbance value) / control group absorbance value)] × 100%.

[0058] (2) Anti-glycation properties of anti-aging agents The bovine serum albumin-glucose glycosylation reaction system was used to simulate non-enzymatic glycosylation reactions in the human body. The inhibitory effect of anti-aging agents on non-enzymatic glycosylation was tested to evaluate the anti-glycation performance of anti-aging agents.

[0059] The specific testing methods are as follows: The anti-aging agents prepared in the examples or comparative examples were formulated into a sample solution with a concentration of 0.1 g / L using deionized water.

[0060] Prepare 10 mL each of a 15 mg / mL bovine serum albumin solution and a 0.3 mol / L glucose solution (diluent is PBS buffer solution containing 1% NaN3, concentration 0.20 mol / L, pH 7.2). Mix the two solutions thoroughly and use as the reaction solution. Then add 10 mL of the anti-aging agent sample solution and incubate at 37°C in the dark for 7 days. Then use a fluorescence microplate reader to measure the fluorescence value RFU at the absorption wavelength of 370 nm / excitation wavelength of 440 nm. Calculate the inhibition rate of non-enzymatic glycosylation according to the following formula: Inhibition rate (%) = [1 - (RFU test sample group - RFU blank group) / RFU negative control group] × 100%.

[0061] Meanwhile, 10 mL of 1 wt% aminoguanidine sulfate was used as a positive control group instead of the anti-aging agent sample solution, and 10 mL of PBS buffer solution containing 1% NaN3, with a concentration of 0.20 mol / L and a pH of 7.4 was used as a negative control group instead of the anti-aging agent sample solution; 20 mL of PBS buffer solution containing 1% NaN3, with a concentration of 0.20 mol / L and a pH of 7.4 was used as a blank group instead of the reaction solution.

[0062] (3) Stability of anti-aging agents The anti-aging formulations prepared in the examples or comparative examples were stored at 4°C for 180 days, and then their antioxidant and anti-glycation properties were tested to evaluate their stability. Stability was calculated using the following formulas: Antioxidant stability (%) = (Antioxidant value of the sample after storage for a period of time) / (Antioxidant value of the original sample) × 100%; Anti-glycation stability (%) = (Anti-glycation value of the sample after storage for a period of time) / (Anti-glycation value of the original sample) × 100%.

[0063] Table 2 Performance test results of the formulations in the examples and comparative examples. As can be seen from the test results in Table 2 above, by using the technical solution provided in this application, human exosomes, plant exosomes and collagen as raw materials, and controlling the amount of each combined raw material, the raw materials can work together and have a synergistic effect. The resulting anti-aging preparation has strong antioxidant and anti-glycation properties, and excellent stability.

[0064] By comparing the test results of Examples 1-5 and Comparative Examples 1-2, it can be seen that the amount of each raw material component has a significant impact on the performance of the product. The anti-aging preparation prepared by this application using 8-12 parts of human exosomes, 3-7 parts of plant exosomes, and 0.5-1.5 parts of collagen has strong antioxidant properties, anti-glycation properties, and stability.

[0065] By comparing the test results of Examples 1 and 6-9, it can be seen that in the preparation method of human exosomes, this application selects to seed human umbilical cord mesenchymal stem cells grown to the logarithmic phase in DMEM / F12 medium containing poloxamer 407 at a concentration of 3-10 ng / mL and 10% fetal bovine serum for proliferation culture, which further improves the performance of anti-aging agents.

[0066] By comparing the test results of Examples 1, 10-11, and Comparative Examples 3-5, it can be seen that in Comparative Example 3, the method for preparing plant exosomes used an equal amount of white peony root instead of beetroot; in Comparative Example 4, the method used an equal amount of aloe vera instead of icewort; and in Comparative Example 5, the weight ratio of beetroot to icewort was 9:1.5, resulting in anti-aging agents with poor performance. In contrast, the anti-aging agent prepared in this application using beetroot and icewort in a weight ratio of 0.5-3:7-11 exhibits strong antioxidant and anti-glycation properties.

[0067] (3) Skin moisture content and anti-wrinkle performance test Application Examples 1-11 Application Examples 1-11 each provide an anti-aging skincare product.

[0068] The anti-aging agents used in Examples 1-11 are derived from Examples 1-11, respectively.

[0069] The preparation method of the anti-aging skin care product is as follows: 55g of water, 7g of glycerin, 1g of VC ethyl ether, and 1g of anti-aging agent are mixed together.

[0070] Testing Method: Female volunteers aged 40-60 years were randomly selected and randomly divided into 13 groups of 5 people each. Before the experiment, skin hydration and wrinkle condition were measured using a skin analyzer. Eleven groups of female volunteers applied the anti-aging skincare products provided in Examples 1-11 above to their faces twice a day, morning and evening, after washing their faces, applying 3g of the anti-aging skincare product each time. The 12th group of volunteers applied the same amount of drinking water and the same amount of anti-aging skincare product without added anti-aging agents (prepared by mixing 55g of water, 7g of glycerin, and 1g of VC ethyl ether). After 28 days, skin hydration and wrinkle condition were measured using a skin analyzer.

[0071] During the testing period, volunteers must not use cosmetics or health products with similar ingredients and functions to the anti-aging skincare products described in this application, and must voluntarily sign an informed consent form.

[0072] Test results are shown in Table 3.

[0073] Table 3 Results of Skin Moisture Content and Anti-wrinkle Performance Testing As shown in Table 2 above, the anti-aging preparation prepared in this application, when used in anti-aging skincare products, resulted in a 64.1-97.1% increase in skin moisture and a 33.2-48.6% reduction in wrinkles after four weeks of application. These results indicate that the anti-aging preparation prepared in this application can significantly increase skin moisture content and reduce wrinkles, thus exhibiting a strong anti-aging effect.

[0074] Although the present invention has been described in detail above with general descriptions and specific embodiments, modifications or improvements can be made to it, which will be obvious to those skilled in the art. Therefore, all such modifications or improvements made without departing from the spirit of the present invention fall within the scope of protection claimed by the present invention.

Claims

1. An anti-aging preparation of human-derived exosome in combination with plant exosome, characterized in that, Specifically includes the following weight parts of components: 8-12 parts of human-derived exosomes, 3-7 parts of plant exosomes, 0.5-1.5 parts of collagen; The preparation method of the plant exosome is as follows: taking beet root and ice leaf as raw materials in a weight ratio of 0.5-3:7-11, crushing, then adding buffer solution, and then sequentially performing cell wall breaking treatment, gradient centrifugation to obtain supernatant, dilution, deep filtration, and concentration and purification.

2. The anti-aging preparation according to claim 1, characterized in that, Specifically includes the following weight parts of components: 9-11 parts of human-derived exosomes, 4-6 parts of plant exosomes, and 0.7-1.2 parts of collagen.

3. The anti-aging preparation according to claim 1, characterized in that, The preparation method of the human-derived exosome is as follows: culturing human umbilical cord mesenchymal stem cells in DMEM / F12 culture medium containing 10% fetal bovine serum, growing to the logarithmic phase; inoculating the human umbilical cord mesenchymal stem cells grown to the logarithmic phase into DMEM / F12 culture medium containing 3-10 ng / mL of poloxamer 407 and 10% fetal bovine serum, and performing proliferation culture for 48-72 h to obtain proliferated human umbilical cord mesenchymal stem cells; aspirating the original culture medium, and then inoculating into serum-free DMEM / F12 culture medium; after 48-72 h of culture, collecting the cell culture supernatant; performing gradient centrifugation on the cell culture supernatant to obtain human umbilical cord mesenchymal stem cell exosomes.

4. Anti-aging preparation according to claim 3, characterized in that The specific steps of the gradient centrifugation are as follows: performing primary centrifugation on the cell culture supernatant at 300-500 g for 8-12 min to remove dead cells; Then performing secondary centrifugation on the supernatant after the primary centrifugation at 3000-5000 g for 10-20 min to remove cell fragments; Then performing tertiary centrifugation on the supernatant after the secondary centrifugation at 8000-12000 g for 20-30 min to remove larger vesicles and apoptotic bodies; Then performing quaternary centrifugation on the supernatant after the tertiary centrifugation at 60000-100000 g for 40-60 min to collect the precipitate, and performing quinary centrifugation on the obtained crude exosomes using PBS buffer at 60000-100000 g for 40-60 min to collect the precipitate, thereby obtaining purified human umbilical cord mesenchymal stem cell exosomes, and obtaining the human umbilical cord mesenchymal stem cell exosomes.

5. The anti-aging preparation according to claim 1, characterized in that, The preparation method of the plant exosome is as follows: taking beet root and ice leaf as raw materials in a weight ratio of 1-2:8-10, crushing, then adding buffer solution, and then sequentially performing cell wall breaking treatment, gradient centrifugation to obtain supernatant, dilution, deep filtration, and concentration and purification.

6. The anti-aging preparation according to claim 1, characterized in that, The preparation method of the plant exosome is as follows: taking beet root and ice leaf as raw materials, grinding, adding PBS buffer solution in a solid-liquid ratio of 1:80-120, then sequentially performing cell wall breaking treatment, gradient centrifugation at 7000-10000 rpm for 40-80 min to obtain supernatant, diluting by adding 10-15 times of PBS buffer solution, performing deep filtration by tangential flow depth filtration membrane, and concentrating and purifying.

7. The anti-aging preparation according to claim 1, characterized in that, The collagen is selected from one or more of rose collagen, bovine collagen peptide, and fish collagen tripeptide.

8. Process for the preparation of an anti-aging preparation according to any one of claims 1-7, characterized in that, Specific steps are: respectively take the corresponding weight of human exosome, plant exosome, collagen, mix evenly, anti-aging preparation is obtained.

9. Use of the anti-aging preparation according to any one of claims 1-7 in the preparation of an anti-aging skin care product.

10. An anti-aging skin care product, characterized by, The anti-aging skin care product comprises the anti-aging preparation according to any one of claims 1-7; the anti-aging skin care product comprises the following components by weight: water 50-60 parts, glycerol 3-10 parts, VC ethyl ether 0.5-2 parts, anti-aging preparation 0.5-1.5 parts.

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