Snake-reading wine capable of inhibiting cancer cell growth and preparation method of snake-reading wine

Abstract

The invention discloses a snake read wine capable of inhibiting cancer cell growth and a preparation method thereof, the snake read wine is prepared by taking white spirit as a main material, adding an agkistrodon halys extract and a zaocys dhumnade extract as auxiliary materials, and performing pretreatment, soaking, clarification, filtration, blending, storage and packaging. The method is simple and suitable for industrialization and large-scale production, zaocys dhumnade and agkistrodon halys are used as main raw materials, the effect of inhibiting various cancer cells is achieved, and good cancer prevention and resisting effects are achieved after long-term drinking.

Description

Technical Field

[0001] This invention relates to the field of snake venom wine production technology, specifically to snake venom wine with the function of inhibiting cancer cell growth and its preparation method. Background Technology

[0002] The global incidence rate of malignant tumors is 148.82 per 100,000 people. In my country, the incidence rate is 211.78 per 100,000 people, with a higher rate in urban areas and a higher prevalence among men. Among men, lung cancer accounts for 28.41%, stomach cancer 15.52%, esophageal cancer 10.39%, colorectal cancer 6.58%, and liver cancer 17.33%, ranking as the top five. Among women, lung cancer accounts for 23.32%, stomach cancer 11.77%, liver cancer 10.98%, colorectal cancer 8.30%, and breast cancer 7.26%, ranking as the top five. For both men and women, the majority of cases occur between the ages of 40 and 80. Cancer has become the leading cause of death.

[0003] Cancer is one of the three major diseases threatening human health, and there is still no effective treatment. Scientists from various countries are exploring snake venom research as a new field to conquer this fortress. The Snake Venom Research Laboratory of China Medical University is attempting to find effective components that can inhibit tumor growth from the venom of vipers from Snake Island in Dalian, Liaoning Province. They conducted a comparative experiment on the tumor inhibition of the original venom and isolated venom of the Snake Island viper. Nine different concentrations of snake venom all showed varying degrees of inhibitory effects on mouse sarcomas, with some tumor inhibition rates as high as 87.1%.

[0004] Viper has anti-inflammatory, analgesic, and antithrombotic effects. It possesses anti-inflammatory properties; its active ingredients can inhibit the production and release of inflammatory factors, thereby alleviating local symptoms such as redness, swelling, heat, and pain. Viper extract contains various alkaloids, which have analgesic effects and can relieve pain caused by inflammation or tissue damage. Certain components in viper can promote blood circulation, reduce blood viscosity, decrease platelet aggregation and fibrinogen deposition, thus preventing thrombosis.

[0005] Black-striped snake (Zaocys dhumnades) is a common traditional Chinese medicine with effects such as dispelling wind and unblocking meridians, relieving spasms, and attacking toxins. It can treat diseases such as rheumatoid arthritis and tetanus. Black-striped snake can also relieve convulsions caused by high fever, tetanus, and eclampsia. It also has a certain detoxifying effect and can be used to treat sores and boils.

[0006] Currently, although there are many products made from rat snake and viper, the overall quality is low and their application in anti-cancer applications is limited. Efforts need to be strengthened to increase the added value of viper and rat snake and broaden the scope of the industry. Summary of the Invention

[0007] To address the problems existing in the prior art, this invention provides a snake wine that inhibits the growth of cancer cells and its preparation method. This product has a good inhibitory effect on a variety of cancer cells, and the preparation method is simple and suitable for industrialization and large-scale production.

[0008] To achieve the above invention, the implementation scheme is as follows: The product is made primarily from baijiu (Chinese liquor), with viper extract and black-striped snake extract added as auxiliary ingredients. It is prepared through pretreatment, soaking, clarification, filtration, blending, storage, and packaging.

[0009] Preferably, the alcohol content of the liquor is between 40 and 70 degrees.

[0010] Preferably, the amount of viper extract added is 1%-20% of the weight of the liquor.

[0011] Preferably, the amount of black snake extract added is 1-25% of the weight of the liquor.

[0012] The method for preparing the Zaocys dhumnades extract is as follows: Take the black-tailed snake, remove the head, scales and ash, soak it thoroughly in a measured amount of yellow rice wine, take it out while it is still wet, remove the skin and bones, cut it into sections, dry it, and sift out the debris; for every 100 kg of black-tailed snake, use 20 kg of yellow rice wine.

[0013] The method for preparing the viper extract is as follows: Take a viper, remove its internal organs, and then dry or roast it until it is finely powdered.

[0014] The invention is characterized by its simple method, suitability for industrialization and large-scale production, and the use of black-striped snake and viper as the main raw materials. It has an inhibitory effect on various cancer cells, and long-term consumption has a good anti-cancer effect. Detailed Implementation

[0015] To better understand the key points of the present invention, the invention will now be further described in detail through specific embodiments. However, the following embodiments should not be construed as further limitations on the present invention. Example 1

[0016] The product is made primarily from baijiu (Chinese liquor), with viper extract and black-striped snake extract added as auxiliary ingredients. It is prepared through pretreatment, soaking, clarification, filtration, blending, storage, and packaging.

[0017] Preferably, the alcohol content of the liquor is 41 degrees.

[0018] Preferably, the amount of viper extract added is 10% of the weight of the liquor.

[0019] Preferably, the amount of black snake extract added is 24% of the weight of the liquor.

[0020] The method for preparing the Zaocys dhumnades extract is as follows: Take the black-tailed snake, remove the head, scales and ash, soak it thoroughly in a measured amount of yellow rice wine, take it out while it is still wet, remove the skin and bones, cut it into sections, dry it, and sift out the debris; for every 100 kg of black-tailed snake, use 20 kg of yellow rice wine.

[0021] The method for preparing the viper extract is as follows: Take a viper, remove its internal organs, and then dry or roast it until it is finely powdered. Example 2

[0022] The product is made primarily from baijiu (Chinese liquor), with viper extract and black-striped snake extract added as auxiliary ingredients. It is prepared through pretreatment, soaking, clarification, filtration, blending, storage, and packaging.

[0023] Preferably, the alcohol content of the liquor is 55%.

[0024] Preferably, the amount of viper extract added is 18% of the weight of the liquor.

[0025] Preferably, the amount of black snake extract added is 2% of the weight of the liquor.

[0026] The method for preparing the Zaocys dhumnades extract is as follows: Take the black-tailed snake, remove the head, scales and ash, soak it thoroughly in a measured amount of yellow rice wine, take it out while it is still wet, remove the skin and bones, cut it into sections, dry it, and sift out the debris; for every 100 kg of black-tailed snake, use 20 kg of yellow rice wine.

[0027] The method for preparing the viper extract is as follows: Take a viper, remove its internal organs, and then dry or roast it until it is finely powdered. Example 3

[0028] The product is made primarily from baijiu (Chinese liquor), with viper extract and black-striped snake extract added as auxiliary ingredients. It is prepared through pretreatment, soaking, clarification, filtration, blending, storage, and packaging.

[0029] Preferably, the alcohol content of the liquor is 68 degrees.

[0030] Preferably, the amount of viper extract added is 2% of the weight of the liquor.

[0031] Preferably, the amount of black snake extract added is 12% of the weight of the liquor.

[0032] The method for preparing the Zaocys dhumnades extract is as follows: Take the black-tailed snake, remove the head, scales and ash, soak it thoroughly in a measured amount of yellow rice wine, take it out while it is still wet, remove the skin and bones, cut it into sections, dry it, and sift out the debris; for every 100 kg of black-tailed snake, use 20 kg of yellow rice wine.

[0033] The method for preparing the viper extract is as follows: Take a viper, remove its internal organs, and then dry or roast it until it is finely powdered.

[0034] Tumor suppression test: Log-phase adherent breast cancer MCF-7 cells and liver cancer HepG2 cells were selected, digested with trypsin, and then prepared into a cell suspension of 5000 cells / ml in RPMI 1640 medium containing 10% fetal bovine serum. The cells were seeded into 96-well plates at 100 μl per well and cultured at 37°C with 5% CO2 until the cells formed a monolayer covering the bottom of the well.

[0035] The experimental group received fresh culture medium containing different concentrations of the sample from the examples, while the control group received culture medium containing an equal volume of solvent. Each group had 3–5 parallel wells and was incubated at 37°C with 5% CO2 for 4–5 days. The supernatant was discarded, and 100 μl of freshly prepared serum-free culture medium containing 0.2 mg / ml MTT was added to each well. Incubation continued at 37°C for 4 hours. The supernatant was carefully discarded, and 100 μl of DMSO was added. After mixing with a micro-ultrasonic oscillator, the optical density was measured on a microplate reader at a test wavelength of 570 nm and a reference wavelength of 450 nm.

[0036] The inhibition rate of the drug on tumor cell growth is calculated using the following formula: Tumor cell growth inhibition rate % = (1 - OD experiment / OD control) × 100% A dose-response curve can be obtained by plotting the tumor cell growth inhibition rate at different concentrations of the same sample, from which the half-maximal killing concentration (IC50) of the sample can be determined.

[0037] As can be seen from the above, the product of the present invention has a significant tumor-suppressing effect.

[0038] The above description is merely a preferred embodiment of the present invention and is not intended to limit the invention in any way. Any person skilled in the art can make many possible variations and modifications to the technical solutions of the present invention, or modify them into equivalent embodiments, without departing from the scope of the present invention. Therefore, any simple modifications, equivalent changes, and alterations made to the above embodiments based on the technical essence of the present invention, without departing from the scope of the present invention, should fall within the protection scope of the present invention.

Claims

1. A method for preparing snake wine that inhibits cancer cell growth, characterized in that: The product is made primarily from baijiu (Chinese liquor), with viper extract and black-striped snake extract added as auxiliary ingredients. It is prepared through pretreatment, soaking, clarification, filtration, blending, storage, and packaging.

2. The method for preparing snake wine that inhibits cancer cell growth according to claim 1, characterized in that: The alcohol content of the liquor is 40-70 degrees.

3. The method for preparing snake wine that inhibits cancer cell growth according to claim 2, characterized in that: The amount of viper extract added is 1%-20% of the weight of the liquor.

4. The method for preparing snake wine that inhibits cancer cell growth according to claim 3, characterized in that: The amount of the black snake extract added is 1-25% of the weight of the liquor.

5. The method for preparing snake wine that inhibits cancer cell growth according to claim 4, characterized in that: The preparation method of the black-tailed snake extract is as follows: take black-tailed snake, remove the head, scales and ash, soak it in a certain amount of rice wine, take it out while it is still wet to remove the skin and bones, cut it into sections, dry it, and sieve out the debris; for every 100 kg of black-tailed snake, use 20 kg of rice wine.

6. The method for preparing snake wine that inhibits cancer cell growth according to claim 5, characterized in that: The method for preparing the viper extract is as follows: take a viper, remove its internal organs, dry or roast it, and grind it into a fine powder.