Foliar fertilizer for inducing gray mold resistance of melon vegetables and preparation method of foliar fertilizer

Abstract

The invention discloses a foliar fertilizer for inducing gray mold resistance of melon vegetables and a preparation method of the foliar fertilizer, and belongs to the technical field of foliar fertilizer.The foliar fertilizer comprises three main components, namely S-allyl-L-cysteine and 2-piperidinecarboxylic acid. As an amino acid potassium salt, potassium glutamate not only provides necessary potassium element for plants, but also enhances the growth vigor and disease resistance of the plants by promoting nitrogen metabolism in the plants. S-allyl-L-cysteine is an amino acid derivative containing a sulfur element and has the effect of inducing activity of resistance-related enzymes in plants, so that the resistance of the plants to gray mold is improved. 2-piperidinecarboxylic acid is a bioactive substance and can stimulate plants to generate disease-resistant related compounds, enhance the structural integrity of plant cells and resist invasion of pathogens. The potassium element provided by the potassium glutamate can enhance the thickness and strength of plant cell walls, and the combination of physical and chemical defense lines enables plants to more effectively resist the attack of gray mold.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of foliage fertilizers, and particularly relates to a foliage fertilizer for inducing resistance of melon vegetables to botrytis and a preparation method thereof. BACKGROUND

[0002] With the rapid development of modern agriculture, the planting area and yield of melon vegetables are continuously increasing. However, botrytis, as one of the main diseases of melon vegetables, seriously affects the yield and quality of vegetables and brings huge economic losses to farmers. At present, the main methods for preventing and treating botrytis include chemical control and biological control. However, chemical control is easy to cause pesticide residues and environmental pollution, and biological control has limited effect and is difficult to meet the demand of large-scale production. As a kind of fertilizer directly applied to the leaves of plants, foliage fertilizer has the advantages of fast absorption and remarkable effect, and has been widely used in plant disease control in recent years.

[0003] However, the existing foliage fertilizer products mainly focus on providing nutrition and lack the function of resistance induction to specific diseases, especially in the prevention and treatment of botrytis, the effect is not ideal. SUMMARY

[0004] The purpose of the present application is to solve the above-mentioned problems, and to provide a foliage fertilizer for inducing resistance of melon vegetables to botrytis and a preparation method thereof.

[0005] The technical scheme adopted by the present application is as follows: a foliage fertilizer for inducing resistance of melon vegetables to botrytis, comprising: potassium glutamate 20-30 parts by weight; S-allyl-L-cysteine 10-15 parts by weight; 2-piperidinecarboxylic acid 5-10 parts by weight; glycine 5-10 parts by weight; seaweed extract 5-10 parts by weight; sodium silicate 3-5 parts by weight; tea tree oil 3-5 parts by weight; ginger extract 3-5 parts by weight; honeysuckle extract 3-5 parts by weight; pepper extract 2-4 parts by weight; wormwood extract 2-4 parts by weight; lemon grass oil 2-4 parts by weight; rosemary extract 2-4 parts by weight.

[0006] In a preferred embodiment, the method comprises the following steps:

[0007] S1: first, weigh the various raw materials of the formula, then slowly add potassium glutamate to an appropriate amount of water, stir while adding, until completely dissolved, form a uniform and transparent base solution. Ensure that the water temperature and stirring speed are appropriate to speed up the dissolution process;

[0008] S2: gradually add S-allyl-L-cysteine to the base solution, continue to stir until completely dissolved, observe the color and transparency of the solution, and ensure that there are no undissolved particles;

[0009] S3: Add 2-piperidinecarboxylic acid, stir well to ensure uniform distribution. Pay attention to the order of addition to avoid adverse reactions between ingredients;

[0010] S4: Add glycine, seaweed extract, and sodium silicate in sequence, and stir well after each addition to ensure uniform distribution of each ingredient. Use emulsifiers or high-speed stirrers to help uniform dispersion, forming a stable emulsion;

[0011] S5: Gently add tea tree oil, ginger extract, honeysuckle extract, pepper extract, wormwood extract, lemon grass oil, and rosemary extract, avoiding vigorous stirring to protect the activity of plant extracts. Ensure that the extracts are evenly distributed in the solution without obvious agglomeration;

[0012] S6: Measure the pH of the solution using a pH meter, and add an appropriate amount of pH adjuster as needed to adjust it to a range suitable for plant growth;

[0013] S7: Filter the mixed solution through filter paper or a filter to remove insoluble substances and impurities, ensuring the purity of the solution. Check the filtered solution to ensure there are no residual impurities;

[0014] S8: Test the stability of the filtered solution by simulating different storage conditions to observe whether the solution separates or deteriorates. Ensure that the product remains uniform and effective after long-term storage;

[0015] S9: Fill the prepared foliar fertilizer solution into clean, dry, sealed containers, and label them with the preparation date and expiration date. Store them in a cool, dry place, away from direct sunlight and high temperatures, for future use.

[0016] In a preferred embodiment, deionized water or distilled water is used in step S1 to avoid impurities affecting the dissolution effect. The water temperature and stirring speed are key parameters, and the water temperature should be controlled between 20-30°C to accelerate the dissolution process without causing decomposition of the ingredients. The stirring speed should be moderate to maintain the uniformity of the solution and avoid the generation of a large amount of bubbles. Stir continuously during the dissolution process for 10-15 minutes to ensure that the potassium glutamate is completely dissolved without visible particles.

[0017] In a preferred embodiment, in step S2, the S-allyl-L-cysteine powder is slowly sprinkled into the solution to avoid clumping caused by adding a large amount at once. Observe the color and transparency of the solution to ensure that there are no undissolved particles. This process may take 15-20 minutes, depending on the particle size and solubility of the powder. If the solution appears turbid or precipitates, extend the stirring time or heat appropriately to 30-35°C.

[0018] In a preferred embodiment, in step S3, the amount of 2-piperidinecarboxylic acid added should be gradually increased, and after each addition, stirring should be performed for 5-10 minutes to ensure complete dissolution and distribution. Attention should be paid to the order of addition to avoid adverse reactions with previous ingredients. The stirring speed should be kept constant to avoid splashing of the solution caused by excessive speed. The total stirring time for this step should not be less than 20 minutes to ensure that the 2-piperidinecarboxylic acid is fully incorporated into the solution.

[0019] In a preferred embodiment, in step S4, the interval between the addition of each ingredient should be 5-10 minutes to ensure that the previous ingredient is completely dissolved before adding the next one. An emulsifier or high-speed stirrer should be used to help uniform dispersion and form a stable emulsion. The amount of emulsifier added should be adjusted according to the viscosity and stability of the solution. The speed of the high-speed stirrer should be set to 3000-5000 revolutions per minute, and the stirring time should not be less than 30 minutes to ensure that all ingredients are uniformly distributed and form a stable emulsion.

[0020] In a preferred embodiment, in step S5, each plant extract should be slowly added to the solution to avoid vigorous stirring to protect the activity of the plant extract. The stirring speed should be slowed down to 100-200 revolutions per minute to prevent the emulsifier from destroying the structure of the plant extract. After each extract is added, stirring should be performed for 10-15 minutes to ensure that the extract is uniformly distributed in the solution without obvious agglomeration. The total time for this step may take 1-2 hours, depending on the type and quantity of the extract.

[0021] In a preferred embodiment, in step S6, the addition of the microbial agent should use a step-by-step dilution method, first mixing the microbial agent with a small amount of solution, and then gradually adding it to the main solution. The stirring speed should be very slow, not more than 50 revolutions per minute, to prevent damage to the bacterial cells. A pH meter should be used to measure the pH value of the solution, and appropriate pH adjusters such as citric acid or sodium hydroxide should be added as needed to adjust the pH to a range suitable for plant growth (usually 6.0-7.0). The pH adjustment should be completed before the addition of the microbial agent to avoid adverse effects on the bacterial cells.

[0022] In step S7, filter paper or filters with a pore size of 0.2-0.5 microns should be selected to remove insoluble substances and impurities. The filtration process should be carried out under sterile conditions to avoid contamination. The filtration speed should be controlled at a moderate speed to avoid clogging of the filter paper or splashing of the solution caused by excessive speed. The filtered solution should be checked for its transparency and purity to ensure that there are no residual impurities. If the filter paper is found to be clogged, a new filter paper should be replaced, and the filtration should continue until the solution is completely clear.

[0023] In a preferred embodiment, in step S8, the different storage conditions, light and vibration are simulated to observe whether the solution is layered or deteriorated. The stability test should last at least one month, during which the solution is checked once a week. Ensure that the product remains uniform and effective after long-term storage. If the solution is found to be layered, precipitated or changed in color, the formula or preparation process should be adjusted to improve the stability of the product.

[0024] In a preferred embodiment, in step S9, the filling process should be carried out under sterile conditions to avoid microbial contamination in the air. The foliar fertilizer solution is transferred to the container using a peristaltic pump or manually poured, avoiding contact between the solution and the container port to reduce the risk of contamination. After filling, the container is immediately sealed to ensure good sealing to prevent air from entering. Label with the preparation date and expiration date, the recommended effective period is 6-12 months. Store in a cool and dry place, avoid direct sunlight and high temperature, ready for use. Shake well before use, if there is a small amount of precipitation, it is a normal phenomenon, which does not affect the use effect. During storage, the container sealing and solution state should be checked regularly to ensure product quality.

[0025] In summary, due to the adoption of the above technical scheme, the application has the following advantages:

[0026] 1、In the present application, S-allyl-L-cysteine and 2-piperidine carboxylic acid are the three main components. Potassium glutamate, as a potassium salt of an amino acid, not only provides essential potassium elements for plants, but also enhances the growth potential and disease resistance of plants by promoting nitrogen metabolism in the plant body. S-allyl-L-cysteine is an amino acid derivative containing sulfur elements, which can induce the activity of resistance-related enzymes in the plant body, thereby improving the resistance of the plant to botrytis cinerea. 2-piperidine carboxylic acid is a bioactive substance that can stimulate the production of disease-resistant compounds in plants and enhance the structural integrity of plant cells to resist the invasion of pathogens. Potassium glutamate provides potassium elements that can enhance the thickness and strength of plant cell walls, providing the first physical defense line for plants; at the same time, potassium elements can also promote the synthesis and activity of disease-resistant enzymes in the plant body, complementing the action of S-allyl-L-cysteine. 2-piperidine carboxylic acid further strengthens the chemical defense line of plants by inducing the production of more disease-resistant compounds. The combination of physical and chemical defense lines enables plants to more effectively resist the invasion of botrytis cinerea.

[0027] 2、In the application, the formula effectively induces the resistance of melon vegetables to gray mold through the joint action of multiple mechanisms. In practical application, this foliar fertilizer not only can reduce the occurrence of diseases, but also can promote the healthy growth of plants, improve yield and quality. According to the actual use situation, farmers can make appropriate adjustments to the formula according to the local climate conditions and soil types to obtain the best use effect. In addition, since the components in the foliar fertilizer are all biologically friendly, it will not pollute the environment and meet the requirements of sustainable development of modern agriculture. Therefore, this foliar fertilizer has wide application prospect and popularization value in melon vegetable planting. BRIEF DESCRIPTION OF DRAWINGS

[0028] Figure 1 It is a flow principle schematic diagram of the application. DETAILED DESCRIPTION

[0029] In order to make the purpose, technical scheme and advantages of the application more clear, the application will be further described in detail below in combination with the drawings and examples. It should be understood that the specific examples described herein are only used to explain the application and not to limit the application.

[0030] Reference Figure 1 ,

[0031] Embodiment:

[0032] A foliar fertilizer for inducing resistance of melon vegetables to gray mold, comprising:

[0033] Potassium glutamate: 30 parts by weight; S-allyl-L-cysteine: 15 parts by weight; 2-piperidinecarboxylic acid: 10 parts by weight; glycine: 10 parts by weight; seaweed extract: 10 parts by weight; sodium silicate: 3 parts by weight; tea tree oil: 3 parts by weight; ginger extract: 5 parts by weight; honeysuckle extract: 3 parts by weight; pepper extract: 2 parts by weight; wormwood extract: 2 parts by weight; lemon grass oil: 2 parts by weight; rosemary extract: 2 parts by weight.

[0034] The method comprises the following steps:

[0035] S1: First, weigh the various raw materials of the formula, then slowly add potassium glutamate to the appropriate amount of water, stir while adding, until completely dissolved, form a uniform and transparent base solution. Ensure that the water temperature and stirring speed are appropriate to speed up the dissolution process;

[0036] S2: Gradually add S-allyl-L-cysteine to the base solution, continue to stir until completely dissolved, observe the color and transparency of the solution, ensure that there are no undissolved particles;

[0037] S3: Add 2-piperidinecarboxylic acid, stir well to ensure uniform distribution. Pay attention to the order of addition to avoid adverse reactions between components;

[0038] S4: Add glycine, seaweed extract, and sodium silicate one by one, and stir well after each addition to ensure uniform distribution of each ingredient. Use emulsifiers or high-speed stirrers to help evenly disperse, forming a stable emulsion;

[0039] S5: Gently add tea tree oil, ginger extract, honeysuckle extract, pepper extract, wormwood extract, lemon grass oil, and rosemary extract, avoiding vigorous stirring to protect the activity of plant extracts. Ensure that the extracts are evenly distributed in the solution without obvious agglomeration;

[0040] S6: Measure the pH of the solution using a pH meter, and add an appropriate amount of pH adjuster as needed to adjust it to a range suitable for plant growth;

[0041] S7: Filter the mixed solution through filter paper or a filter to remove insoluble substances and impurities, ensuring the purity of the solution. Check the filtered solution to ensure there are no residual impurities;

[0042] S8: Test the stability of the filtered solution by simulating different storage conditions to observe whether the solution separates or deteriorates. Ensure that the product remains uniform and effective after long-term storage;

[0043] S9: Fill the prepared foliar fertilizer solution into clean, dry, sealed containers, and label them with the preparation date and expiration date. Store them in a cool, dry place, away from direct sunlight and high temperatures, for future use.

[0044] In step S1, use deionized or distilled water to avoid impurities affecting the dissolution effect. The water temperature and stirring speed are key parameters, and the water temperature should be controlled between 20-30°C to accelerate the dissolution process without causing ingredient decomposition. The stirring speed should be moderate to maintain the uniformity of the solution and avoid generating a large amount of bubbles. Stir continuously for 10-15 minutes during the dissolution process to ensure that the potassium glutamate is completely dissolved without visible particles.

[0045] In step S2, slowly sprinkle the S-allyl-L-cysteine powder into the solution to avoid clumping caused by adding a large amount at once. Observe the color and transparency of the solution to ensure that there are no undissolved particles. This process may take 15-20 minutes, depending on the particle size and solubility of the powder. If the solution appears turbid or precipitates, extend the stirring time or heat it to 30-35°C as appropriate.

[0046] In step S3, the amount of 2-piperidinecarboxylic acid added should be gradually increased, and stirred for 5-10 minutes after each addition to ensure complete dissolution and distribution. Pay attention to the order of addition to avoid adverse reactions with previous ingredients. The stirring speed should be kept constant to avoid splashing the solution. The total stirring time for this step should not be less than 20 minutes to ensure that the 2-piperidinecarboxylic acid is completely incorporated into the solution.

[0047] In step S4, the interval between adding each ingredient should be 5-10 minutes to ensure that the previous ingredient is fully dissolved before adding the next. An emulsifier or high-speed stirrer can be used to help distribute evenly and form a stable emulsion. The amount of emulsifier added should be adjusted according to the viscosity and stability of the solution. The speed of the high-speed stirrer should be set to 3000-5000 revolutions per minute, and the stirring time should not be less than 30 minutes to ensure that all ingredients are evenly distributed and form a stable emulsion.

[0048] In step S5, each plant extract should be slowly added to the solution, avoiding vigorous stirring to protect the activity of the plant extract. The stirring speed should be slowed down to 100-200 revolutions per minute to prevent the emulsifier from damaging the structure of the plant extract. After each extract is added, stir for 10-15 minutes to ensure that the extract is evenly distributed in the solution without obvious agglomeration. The total time for this step may take 1-2 hours, depending on the type and quantity of the extract.

[0049] In step S6, the addition of the microbial agent should use a step-by-step dilution method, first mixing the microbial agent with a small amount of solution, and then gradually adding it to the main solution. The stirring speed should be very slow, not more than 50 revolutions per minute, to prevent damage to the bacterial cells. Use a pH meter to measure the pH value of the solution, and add an appropriate amount of pH adjuster such as citric acid or sodium hydroxide as needed to adjust it to the range suitable for plant growth (usually 6.0-7.0). pH adjustment should be completed before the addition of the microbial agent to avoid adverse effects on the bacterial cells.

[0050] In step S7, choose filter paper or filters with a pore size of 0.2-0.5 microns to remove insoluble substances and impurities. The filtration process should be carried out under sterile conditions to avoid contamination. The filtration speed should be controlled at a moderate speed to avoid too fast causing the filter paper to block or the solution to splash. The filtered solution should be checked for its transparency and purity to ensure that there are no residual impurities. If the filter paper is found to be blocked, replace it with a new one and continue filtering until the solution is completely clear.

[0051] In step S8, simulate different storage conditions such as temperature (4℃, 25℃, 40℃), light (dark, light) and vibration, and observe whether the solution is layered or deteriorated. The stability test should last at least one month, during which the solution should be checked once a week. Ensure that the product remains uniform and effective after long-term storage. If the solution is found to be layered, precipitated or changed in color, the formula or preparation process should be adjusted to improve the stability of the product.

[0052] In step S9, the filling process should be carried out under sterile conditions to avoid microbial contamination from the air. Use a peristaltic pump or manually pour the foliar fertilizer solution into the container, avoiding contact between the solution and the container opening as much as possible to reduce the risk of contamination. After filling, immediately seal the container to ensure good sealing to prevent air from entering. Label with the preparation date and expiration date, and the recommended effective period is 6-12 months. Store in a cool and dry place, avoid direct sunlight and high temperature, ready for use. Shake well before use, if there is a small amount of sediment, it is a normal phenomenon, does not affect the use effect. During storage, the container sealing and solution state should be checked regularly to ensure product quality.

[0053] Test Example: Evaluation of Foliar Fertilizer Effect on Inducing Resistance to Cucumber Gray Mold

[0054] I. Purpose of the test:

[0055] Evaluate the beneficial effect of the foliar fertilizer formula in this application on the resistance of cucumber to gray mold. Through comparative tests, analyze the influence of foliar fertilizer on plant growth and disease resistance.

[0056] II. Test materials:

[0057] Cucumber varieties: Choose common cucumber varieties susceptible to gray mold in the local area, such as cucumbers, watermelons, etc.

[0058] Foliar fertilizer: foliar fertilizer for inducing resistance to cucumber gray mold prepared according to the above formula.

[0059] Control group: Conventional fertilization management, no foliar fertilizer.

[0060] Gray mold spore suspension: used for inoculation, simulating natural infection conditions.

[0061] III. Test method:

[0062] Set up test groups and control groups, with three replicates in each group, a total of 6 plots.

[0063] After the cucumber grows to the seedling stage, the test group starts to spray foliar fertilizer, spraying every 7 days, a total of 3 times.

[0064] The control group is managed conventionally and does not spray foliar fertilizer.

[0065] 7 days after the last spraying of foliar fertilizer, inoculate the gray mold spore suspension to all plots.

[0066] After inoculation, observe and record the disease development, and count the disease index and plant growth indicators at 14 days and 28 days after inoculation.

[0067] IV. Data statistics and analysis:

[0068] The test data is recorded and counted by using the following table:

[0069] Disease index = ∑(number of diseased leaves at each level x relative value) / (total number of leaves surveyed x 9) x 100;

[0070]

[0071] V. Results and analysis

[0072] Disease index: The disease index of the test group was significantly lower than that of the control group at 14 days and 28 days after inoculation, indicating that the foliar fertilizer effectively induced the resistance of the plant to gray mold.

[0073] Plant growth index: The plant height of the test group was higher than that of the control group at 14 days and 28 days after inoculation, indicating that the foliar fertilizer promoted the growth of the plant.

[0074] From the above, it can be seen that in the present application, S-allyl-L-cysteine and 2-piperidine carboxylic acid are the three main components. Potassium glutamate, as an amino acid potassium salt, not only provides essential potassium elements for plants, but also enhances the growth potential and disease resistance of plants by promoting nitrogen metabolism in the plant body. S-allyl-L-cysteine is an amino acid derivative containing sulfur elements, which has the effect of inducing the activity of resistance-related enzymes in the plant body, thereby improving the resistance of the plant to gray mold. 2-piperidine carboxylic acid is a bioactive substance that can stimulate the production of disease-resistant compounds in plants and enhance the structural integrity of plant cells to resist the invasion of pathogens. Potassium elements provided by potassium glutamate can enhance the thickness and strength of plant cell walls, providing the first physical defense line for plants; at the same time, potassium elements can also promote the synthesis and activity of disease-resistant related enzymes in the plant body, complementing the action of S-allyl-L-cysteine. 2-piperidine carboxylic acid further strengthens the chemical defense line of the plant by inducing the production of more disease-resistant compounds. The combination of physical and chemical defense lines enables the plant to more effectively resist the invasion of gray mold.

[0075] In the present application, the formula effectively induces the resistance of melon vegetables to gray mold through the combined action of multiple mechanisms. In practical application, this foliar fertilizer not only reduces the occurrence of diseases, but also promotes the healthy growth of plants, improves yield and quality. According to the actual use situation, farmers can make appropriate adjustments to the formula according to the local climate conditions and soil types to obtain the best use effect. In addition, since the components in the foliar fertilizer are biologically friendly, they will not pollute the environment, meeting the requirements of modern agricultural sustainable development. Therefore, this foliar fertilizer has wide application prospects and promotional value in melon vegetable planting.

[0076] It is to be understood that the phrases such as first and second, and the like, refer to different entities or operations without necessarily requiring or implying any actual relationship or order between such entities or operations. Moreover, the terms "comprises" or "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but can include other elements not expressly listed or inherent to such process, method, article, or apparatus. An element proceeded by "comprises... a" does not, without more constraints, exclude the presence of additional identical elements in the process, method, article, or apparatus that comprises the element.

[0077] The description as set forth herein makes apparent to those skilled in the art the nature of the application, the manner of using it, and the best mode for making and using it. Various modifications and changes can be made to the embodiments described herein without departing from the spirit or scope of the application. Accordingly, it is intended that all such modifications and changes be included within the scope of the application as defined in the following claims.

Claims

1. A foliar fertilizer for inducing resistance to gray mold in cucurbit vegetables, characterized in that: include: Potassium glutamate: 20-30 parts by weight; S-allyl-L-cysteine: 10-15 parts by weight; 2-Piperidinic acid: 5-10 parts by weight; Glycine: 5-10 parts by weight; seaweed extract: 5-10 parts by weight; sodium silicate: 3-5 parts by weight; tea tree oil: 3-5 parts by weight; ginger extract: 3-5 parts by weight; honeysuckle extract: 3-5 parts by weight; Sichuan pepper extract: 2-4 parts by weight; mugwort extract: 2-4 parts by weight; lemongrass oil: 2-4 parts by weight; rosemary extract: 2-4 parts by weight.

2. The method for preparing a foliar fertilizer for inducing gray mold resistance in cucurbit vegetables as described in claim 1, characterized in that: The method includes the following steps: S1: First, weigh all the ingredients in the formula. Then, slowly add potassium glutamate to an appropriate amount of water while stirring until it is completely dissolved and forms a uniform and transparent base solution. Ensure that the water temperature and stirring speed are appropriate to accelerate the dissolution process. S2: Gradually add S-allyl-L-cysteine ​​to the base solution, stirring continuously until completely dissolved. Observe the color and transparency of the solution to ensure there are no undissolved particles. S3: Add 2-piperidinecarboxylic acid and stir thoroughly to ensure even distribution; pay attention to the order of addition to avoid adverse reactions between components; S4: Add glycine, seaweed extract and sodium silicate in sequence, stirring thoroughly after each addition to ensure even distribution of each ingredient; use an emulsifier or a high-speed mixer to help disperse evenly and form a stable emulsion. S5: Gently add tea tree oil, ginger extract, honeysuckle extract, Sichuan pepper extract, artemisia extract, lemongrass oil, and rosemary extract, avoiding vigorous stirring to protect the activity of the plant extracts; ensure that the extracts are evenly distributed in the solution without obvious agglomeration; S6: Use a pH meter to measure the pH value of the solution, and add an appropriate amount of pH adjuster as needed to adjust it to a range suitable for plant growth; S7: Filter the mixed solution through filter paper or a filter to remove insoluble matter and impurities, ensuring the purity of the solution; check the filtered solution to ensure there are no residual impurities; S8: Conduct stability tests on the filtered solution, simulating different storage conditions, and observe whether the solution separates into layers or deteriorates; ensure that the product remains homogeneous and effective after long-term storage; S9: Pour the prepared foliar fertilizer solution into a clean, dry, sealed container, affix a label indicating the preparation date and expiration date; store in a cool, dry place, avoiding direct sunlight and high temperatures, until ready for use.

3. The method for preparing a foliar fertilizer for inducing gray mold resistance in cucurbit vegetables as described in claim 1, characterized in that: In step S1, deionized water or distilled water is used to avoid impurities affecting the dissolution effect; water temperature and stirring speed are key parameters. The water temperature is controlled between 20-30℃ to accelerate the dissolution process without causing component decomposition; the stirring speed is moderate to maintain the homogeneity of the solution and avoid generating a large number of bubbles; the stirring is continued for 10-15 minutes during the dissolution process to ensure that the potassium glutamate is completely dissolved and there are no visible particles.

4. The method for preparing a foliar fertilizer for inducing gray mold resistance in cucurbit vegetables as described in claim 1, characterized in that: In step S2, S-allyl-L-cysteine ​​powder is slowly sprinkled into the solution to avoid clumping caused by adding a large amount at once; the color and transparency of the solution are observed to ensure that there are no undissolved particles. This process takes 15-20 minutes, depending on the particle size and solubility of the powder. If the solution becomes cloudy or precipitates, extend the stirring time or heat it to 30-35°C.

5. The method for preparing a foliar fertilizer for inducing gray mold resistance in cucurbit vegetables as described in claim 1, characterized in that: In step S3, the amount of 2-piperidinecarboxylic acid added is gradually increased, and the mixture is stirred for 5-10 minutes after each addition to ensure full dissolution and distribution. Attention should be paid to the order of addition to avoid adverse reactions with previous components. The stirring speed should be kept constant to avoid splashing of the solution due to excessive speed. The total stirring time in this step should not be less than 20 minutes to ensure that 2-piperidinecarboxylic acid is completely incorporated into the solution.

6. The method for preparing a foliar fertilizer for inducing gray mold resistance in cucurbit vegetables as described in claim 1, characterized in that: In step S4, each component is added at intervals of 5-10 minutes to ensure that the previous component is completely dissolved before adding the next one; an emulsifier or a high-speed mixer is used to help disperse the components evenly and form a stable emulsion; the amount of emulsifier added is adjusted according to the viscosity and stability of the solution; the speed of the high-speed mixer is set to 3000-5000 rpm, and the stirring time is not less than 30 minutes to ensure that all components are evenly distributed and form a stable emulsion.

7. The method for preparing a foliar fertilizer for inducing gray mold resistance in cucurbit vegetables as described in claim 1, characterized in that: In step S5, each plant extract is slowly added dropwise to the solution, avoiding vigorous stirring to protect the activity of the plant extract; the stirring speed is reduced to 100-200 rpm to prevent the emulsifier from damaging the structure of the plant extract; after each extract is added, the mixture is stirred for 10-15 minutes to ensure that the extract is evenly distributed in the solution without obvious agglomeration; the total time for this step is 1-2 hours, depending on the type and quantity of extract.

8. The method for preparing a foliar fertilizer for inducing gray mold resistance in cucurbit vegetables as described in claim 1, characterized in that: In step S6, the bacterial agent is added using a stepwise dilution method. First, the bacterial agent is mixed with a small amount of solution, and then gradually added to the main solution. The stirring speed is very slow, not exceeding 50 rpm, to prevent damage to the bacterial cells. The pH value of the solution is measured using a pH meter, and an appropriate amount of pH adjuster, such as citric acid or sodium hydroxide, is added as needed to adjust it to a range suitable for plant growth. pH adjustment is completed before the bacterial agent is added to avoid adverse effects on the bacterial cells. In step S7, filter paper or filter with a pore size of 0.2-0.5 micrometers is selected to remove insoluble matter and impurities; The filtration process is carried out under sterile conditions to avoid contamination; Keep the filtration speed at a medium speed to avoid clogging the filter paper or splashing of the solution due to excessive speed. The filtered solution is checked for transparency and purity to ensure there are no residual impurities; If the filter paper becomes clogged, replace it with a new one and continue filtering until the solution is completely clear.

9. The method for preparing a foliar fertilizer for inducing gray mold resistance in cucurbit vegetables as described in claim 1, characterized in that: In step S8, different storage conditions are simulated to observe whether the solution separates into layers or deteriorates; the stability test lasts for at least one month, during which the state of the solution is checked weekly. Ensure the product remains uniform and effective even after long-term storage; If the solution shows signs of stratification, precipitation, or color change, adjust the formulation or preparation process to improve product stability.

10. The method for preparing a foliar fertilizer for inducing gray mold resistance in cucurbit vegetables as described in claim 1, characterized in that: In step S9, the filling process is carried out under aseptic conditions to avoid microbial contamination from the air. The foliar fertilizer solution is transferred to the container using a peristaltic pump or by manual pouring, minimizing contact between the solution and the container opening to reduce the risk of contamination. After filling, the container is immediately sealed to ensure good airtightness and prevent air from entering. A label is affixed indicating the preparation date and expiration date, with a recommended shelf life of 6-12 months. Store in a cool, dry place, avoiding direct sunlight and high temperatures, until ready for use. Shake well before use; a small amount of sediment is normal and does not affect the effectiveness. During storage, regularly check the container's seal and the solution's condition to ensure product quality.

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