Composite microbial agent for rapid decomposition of livestock and poultry manure, and preparation method and application thereof

Abstract

The invention discloses a compound microbial agent for rapid decomposition of livestock and poultry manure and a preparation method and application thereof, and belongs to the technical field of microorganisms. The compound microbial agent is prepared from 30 to 40 parts of compound microbial fermentation liquor, 40 to 50 parts of organic carrier and conditioner, 5 to 8 parts of nutritional additive, 3 to 5 parts of mineral element-containing additive and 2 to 4 parts of accelerant, wherein the compound microbial fermentation liquor is prepared from geobacillus thermophilus, high-temperature-resistant heterobacillus and arthrobacter tropion. Three selected strains of the compound microbial agent have a remarkable synergistic effect, and geobacillus thermophilus and high-temperature-resistant heterobacillus lead to a high-temperature stage, so that various organic matters are quickly decomposed, and pathogenic bacteria and worm eggs are killed; nourishing arthrobacter dominates the medium and low temperature stage, thorough deodorization is achieved, and humification is completed. The three components cooperate to realize full-period coverage in the sheep manure composting process, the composting effect is good, the composted substances are loose and odorless, and the seed germination index is high.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of microbial technology, and particularly relates to a compound microbial agent for rapid composting of livestock and poultry manure, a preparation method and application thereof. BACKGROUND

[0002] With the rapid development of large-scale and intensive breeding industry, the amount of livestock and poultry manure is increasing rapidly. If not properly treated, it will not only cause serious environmental pollution such as water eutrophication, air odor, soil compaction, etc., but also lead to the spread of pathogenic bacteria and parasitic eggs, endangering human and animal health. The traditional composting method is one of the main ways to treat livestock and poultry manure, but it has problems such as long fermentation period, large nutrient loss, incomplete composting, heavy odor, etc.

[0003] The traditional composting process of manure relies on natural microorganisms, and has problems such as long fermentation period, slow temperature rise, and heavy odor. Microbial agent technology is an effective way to solve the above problems. By adding exogenous high-efficiency functional microorganisms, the decomposition of organic matter can be accelerated, the composting period can be shortened, the composting quality can be improved, and the odor can be eliminated. There are many related microbial agents in the prior art, but most of them have the shortcomings of single functional strain, poor environmental adaptability, especially insufficient high-temperature resistance, poor compound synergistic effect, etc., resulting in unstable effect in actual application, especially low treatment efficiency for fresh manure with high moisture content in cold seasons in the north.

[0004] Therefore, it is of great significance to develop a compound microbial agent with fast temperature rise, strong decomposition ability, significant deodorization effect, and applicability to various environmental conditions, for promoting the resource utilization of livestock and poultry manure. SUMMARY

[0005] The purpose of the present application is to provide a compound microbial agent for rapid composting of livestock and poultry manure, which has strong decomposition ability, significant deodorization effect, and good composting effect.

[0006] To achieve the above technical purpose, the technical solution adopted by the present application is: A compound microbial agent for rapid composting of livestock and poultry manure, comprising the following raw materials by weight: 30-40 parts of compound microbial fermentation liquor, 40-50 parts of organic carrier and conditioner, 5-8 parts of nutritional adjuvant, 3-5 parts of mineral element-containing adjuvant, and 2-4 parts of promoter.

[0007] Further, the complex microbial fermentation liquor comprises Geobacillus thermoleovorans, Aliibacillus thermotolerans, and Arthrobacter pascens. The Geobacillus thermoleovorans is purchased from the China Center for Type Culture Collection, with a strain number of CCTCC WB 20081274 and an original preservation date of December 16, 2007. The Aliibacillus thermotolerans is purchased from the China General Microbiological Culture Collection Center, with a strain number of CGMCC 1.15790 and an original preservation date of July 10, 2016. The Arthrobacter pascens is purchased from the China General Microbiological Culture Collection Center, with a strain number of CGMCC 1.6310 and an original preservation date of May 12, 2006.

[0008] Further, the preparation method of the complex microbial fermentation liquor is as follows: The Geobacillus thermoleovorans, the Aliibacillus thermotolerans, and the Arthrobacter pascens are activated and cultured separately, and fermentation liquors with a viable bacterial count of 1×10 9 CFU / ml are obtained. The three fermentation liquors are mixed in a volume ratio of 1:1:1 to obtain the complex microbial fermentation liquor.

[0009] Further, the organic carrier and the conditioner are bentonite and mushroom residue, and the mass ratio of the two is (1-2):1.

[0010] Further, the mushroom residue is sieved through a 20-mesh sieve, and the water content is adjusted to 30-40%.

[0011] Further, the nutritional aid comprises molasses and soybean meal, and the mass ratio of the two is (4-6):1.

[0012] Further, the soybean meal is crushed and sieved through a 60-mesh sieve.

[0013] Further, the mineral element-containing aid is superphosphate.

[0014] Further, the accelerator is humic acid and ferrous sulfate, and the mass ratio of the two is (2-4):1.

[0015] Further, the humic acid is sieved through a 100-mesh sieve.

[0016] A preparation method of a complex microbial agent for rapid composting of livestock and poultry manure comprises the following steps: (1) First, a complex microbial fermentation liquor is prepared, and then molasses is diluted with 50°C warm water, and then mixed uniformly with the complex microbial fermentation liquor to form a liquid mixture; (2) Put bentonite, mushroom residue, soybean meal, superphosphate, humic acid and ferrous sulfate into the mixing machine, and stir for 30-45 min, so that all dry materials are fully mixed and uniform; (3) Under the condition of continuous stirring of the mixing machine, the liquid mixture prepared in step (1) is uniformly sprayed on the dry materials of step (2), and stirring is performed until the liquid components are uniformly adsorbed by the dry materials, then the mixture is placed in a granulator for granulation, so that solid particles with a particle size of 3-5 mm are obtained, and low-temperature drying is performed to obtain the compound microbial inoculant.

[0017] The application also provides an application of the compound microbial inoculant for rapid composting of livestock and poultry manure, which is suitable for treating sheep manure and other livestock and poultry manure.

[0018] The thermophilic Bacillus can efficiently degrade cellulose and significantly accelerate the composting process. The thermophilic Bacillus can resist extreme high temperature up to 75℃, can quickly decompose macromolecular organic matters such as proteins and starches, and can generate a large amount of heat to rapidly heat the heap.

[0019] The high-temperature-resistant Bacillus heterozygous can assist the thermophilic Bacillus to efficiently degrade cellulose, hemicellulose and other difficult-to-decompose organic matters in the high-temperature period, and maintain the decomposition activity in the high-temperature period.

[0020] The trophoblast bacillus can also effectively decompose residual organic matter, and has strong deodorization ability, and can effectively degrade ammonia, hydrogen sulfide and other foul-smelling substances.

[0021] The humic acid and ferrous sulfate added in the application can catalyze and stabilize the formation of humus, the superphosphate can not only adjust the pH, but also can combine with nitrogen elements to reduce the loss of nitrogen in the form of ammonia, so as to improve the nutrient content and quality of the final composting product, so that the composting is completely realized and the fertilizer preservation effect is good.

[0022] The compound microbial inoculant has wide applicability, the functional strains in the inoculant are resistant to high temperature and poor nutrition, and the compounded carrier and conditioner can effectively adjust the air permeability of the manure, so that the inoculant is suitable for treating various livestock and poultry manure such as sheep manure, and can exhibit stable performance under different climate conditions.

[0023] Advantages The three selected functional strains have significant synergistic effect, the thermophilic Bacillus and the high-temperature-resistant Bacillus heterozygous dominate the high-temperature stage, quickly decompose various organic matters and kill pathogenic bacteria and insect eggs, and the trophoblast bacillus dominates the medium and low-temperature stage, completely deodorizes and completes humification. The three strains are synergistic, realize the full cycle coverage of the composting process, and the efficiency is much higher than that of single inoculant or unreasonable inoculant, the composting effect is good, the composting material is loose, and the seed germination index is high.

[0024] The compound microbial agent has the advantages of fast temperature rise, long high-temperature duration, effective killing of Escherichia coli and insect eggs, great reduction of pathogen transmission risk, harmless realization, effective shortening of composting period, efficient degradation of foul-smelling substances, and significant deodorization effect. DETAILED DESCRIPTION The technical solutions of the present application are further described below in combination with specific examples, but are not limited thereto.

[0025] Example 1 The compound microbial agent for rapid composting of livestock and poultry manure comprises the following raw materials in parts by weight: 30 parts of compound microbial fermentation liquor, 40 parts of organic carrier and conditioner, 5 parts of nutritional aid, 3 parts of mineral element-containing aid, and 2 parts of promoter.

[0026] The compound microbial fermentation liquor comprises Geobacillus thermoleovorans, Aliibacillus thermotolerans, and Arthrobacter pascens; the strain number of the Geobacillus thermoleovorans is CCTCC WB 20081274; the strain number of the Aliibacillus thermotolerans is CGMCC 1.15790; and the strain number of the Arthrobacter pascens is CGMCC 1.6310.

[0027] The preparation method of the compound microbial fermentation liquor is as follows: The Geobacillus thermoleovorans, the Aliibacillus thermotolerans, and the Arthrobacter pascens are respectively activated and subcultured to obtain fermentation liquors with a viable bacterial count of 1×10 9 CFU / ml, and the three kinds of fermentation liquors are mixed in a volume ratio of 1:1:1 to obtain the compound microbial fermentation liquor.

[0028] The organic carrier and conditioner are bentonite and mushroom residue, and the mass ratio of the two is 1:1.

[0029] The mushroom residue is sieved through a 20-mesh sieve, and the water content is adjusted to 30%.

[0030] The nutritional aid comprises molasses and soybean meal, and the mass ratio of the two is 4:1.

[0031] The soybean meal is crushed and sieved through a 60-mesh sieve.

[0032] The mineral element-containing aid is superphosphoric acid.

[0033] The promoter is humic acid and ferrous sulfate, and the mass ratio of the two is 2:1.

[0034] The humic acid is sieved through a 100-mesh sieve.

[0035] A preparation method of a complex microbial agent for rapid composting of livestock and poultry manure, comprising the following steps: (1) First, prepare a complex microbial fermentation broth, then dilute molasses with 50℃ warm water, and then mix the molasses with the complex microbial fermentation broth uniformly to form a liquid mixture; (2) Put bentonite, mushroom residue, soybean meal, superphosphate, humic acid and ferrous sulfate into a mixer, stir for 30 min, and mix all the dry materials uniformly; (3) Under the condition of continuous stirring of the mixer, uniformly spray the liquid mixture prepared in step (1) on the dry materials in step (2), stir until the liquid components are uniformly adsorbed by the dry materials, and then put them into a granulator for granulation to obtain solid particles with a particle size of 3-5 mm, and then dry at low temperature to obtain the complex microbial agent.

[0036] Example 2 A complex microbial agent for rapid composting of livestock and poultry manure, comprising the following raw materials by weight: complex microbial fermentation broth 35 parts, organic carrier and conditioner 45 parts, nutritional aid 7 parts, mineral element-containing aid 4 parts, and promoter 3 parts.

[0037] The complex microbial fermentation broth comprises Geobacillus thermoleovorans, Aliibacillus thermotolerans, and Arthrobacter pascens; the strain number of the Geobacillus thermoleovorans is CCTCC WB 20081274; the strain number of the Aliibacillus thermotolerans is CGMCC 1.15790; and the strain number of the Arthrobacter pascens is CGMCC 1.6310.

[0038] The preparation method of the complex microbial fermentation broth is as follows: The Geobacillus thermoleovorans, Aliibacillus thermotolerans, and Arthrobacter pascens are activated and cultured separately, and fermentation broths with a viable bacterial count of 1×10 9 CFU / ml are obtained, and the three fermentation broths are mixed in a volume ratio of 1:1:1 to obtain the complex microbial fermentation broth.

[0039] The organic carrier and conditioner are bentonite and mushroom residue, and the mass ratio of the two is 2:1.

[0040] The mushroom residue is passed through a 20-mesh sieve, and the moisture content is adjusted to 35%.

[0041] The nutritional aid comprises molasses and soybean meal, and the mass ratio of the two is 5:1.

[0042] The soybean meal is crushed through a 60-mesh screen.

[0043] The mineral element-containing auxiliary agent is calcium superphosphate.

[0044] The accelerant is humic acid and ferrous sulfate, and the mass ratio of the two is 3:1.

[0045] The humic acid is passed through a 100-mesh screen.

[0046] A preparation method of a compound microbial agent for rapid composting of livestock and poultry manure, comprising the following steps: (1) first preparing a compound microbial fermentation liquor, then diluting molasses with 50℃ warm water, and then mixing the molasses with the compound microbial fermentation liquor uniformly to form a liquid mixture; (2) putting bentonite, mushroom residue, soybean meal, calcium superphosphate, humic acid and ferrous sulfate into a mixer, stirring for 35 min, and fully mixing all the dry materials uniformly; (3) under the condition of continuous stirring of the mixer, uniformly spraying the liquid mixture prepared in step (1) on the dry materials in step (2), stirring until the liquid components are uniformly adsorbed by the dry materials, placing in a granulator for granulation, obtaining solid particles with a particle size of 3-5 mm, and low-temperature drying to obtain the compound microbial agent.

[0047] Example 3 A compound microbial agent for rapid composting of livestock and poultry manure, comprising the following raw materials by weight: compound microbial fermentation liquor 40 parts, organic carrier and conditioner 50 parts, nutrient auxiliary agent 8 parts, mineral element-containing auxiliary agent 5 parts, and accelerant 4 parts.

[0048] The compound microbial fermentation liquor comprises Geobacillus thermoleovorans, Aliibacillus thermotolerans and Arthrobacter pascens; the Geobacillus thermoleovorans strain has a strain number of CCTCC WB 20081274; the Aliibacillus thermotolerans strain has a strain number of CGMCC 1.15790; and the Arthrobacter pascens strain has a strain number of CGMCC 1.6310.

[0049] The preparation method of the compound microbial fermentation liquor is as follows: The Geobacillus thermoleovorans, Aliibacillus thermotolerans and Arthrobacter pascens are respectively activated and expanded in culture, and fermentation liquors with a viable bacterial count of 1×10 9 CFU / ml are obtained, and the three kinds of fermentation liquors are mixed in a volume ratio of 1:1:1 to obtain the compound microbial fermentation liquor.

[0050] The organic carrier and conditioning agent are bentonite and mushroom residue, and the mass ratio of the two is 1:1.

[0051] The mushroom residue is screened through a 20-mesh sieve, and the moisture content is adjusted to 40%.

[0052] The nutritional aid includes molasses and soybean meal, and the mass ratio of the two is 6:1.

[0053] The soybean meal is crushed through a 60-mesh sieve.

[0054] The mineral element-containing aid is superphosphoric acid.

[0055] The accelerator is humic acid and ferrous sulfate, and the mass ratio of the two is 4:1.

[0056] The humic acid is screened through a 100-mesh sieve.

[0057] A preparation method of a compound microbial agent for rapid composting of livestock and poultry manure, comprising the following steps: (1) First, prepare a compound microbial fermentation liquor, then dilute the molasses with 50°C warm water, and then mix the molasses with the compound microbial fermentation liquor uniformly to form a liquid mixture; (2) Put bentonite, mushroom residue, soybean meal, superphosphoric acid, humic acid and ferrous sulfate into a mixer, stir for 45 minutes, and mix all the dry materials uniformly; (3) Under the condition of continuous stirring of the mixer, uniformly spray the liquid mixture prepared in step (1) on the dry materials in step (2), stir until the liquid components are uniformly adsorbed by the dry materials, put them into a granulator for granulation, obtain solid particles with a particle size of 3-5mm, and dry at low temperature to obtain the compound microbial agent.

[0058] Comparative Example 1 In each comparative example, the volume dosage proportion of the fermentation liquor of Bacillus thermophilus, Bacillus acidophilus and Bacteroides capillosus in the preparation of the compound microbial fermentation liquor is changed, and the remaining raw materials and steps are the same as in Example 1.

[0059] Table 1: Fermentation liquor dosage composition of each comparative example Performance test The tested livestock and poultry manure is sheep manure.

[0060] The test has 13 treatment groups: adding the compound microbial agents prepared in Examples 1-3 and Comparative Examples 1-9 and natural composting (blank control). Mix the sheep manure with 0.5% of each treatment group of microbial agent uniformly, adjust the moisture content to 55%-60%, and stack in a strip pile for fermentation. Insert a thermometer to record the temperature, and record the appearance of the livestock and poultry manure after 5 days of composting. When the temperature rises above 60°C, turn the pile, and until the temperature stabilizes, the composting is completed.

[0061] After the end of the decomposition, 5-point mixed sampling method was used to collect samples, about 0.5 kg was collected at each point. Organic matter was determined by potassium dichromate volumetric method; total nitrogen was determined by Kjeldahl apparatus; total phosphorus was determined by molybdenum-antimony anti-colorimetric method; total potassium was determined by flame spectrophotometer; 3 replicates were taken each time during measurement; Seed germination index determination: 10 g of fresh sheep manure of each treatment group after decomposition was completed, distilled water was added according to the solid-liquid ratio of 1:10, each sample was placed in a 30℃, 180rpm shaking for 30min, 6000rpm centrifugation for 15min, 5ml supernatant was removed and placed in a culture dish with two layers of filter paper, then 20 cabbage seeds were evenly placed in a 25℃ incubator for 48h, distilled water was used as the control group, after 48h, the root length and seed germination number were measured, and the seed germination index was calculated according to the following formula: Seed germination index = (treatment group seed germination rate x treatment group root length) / (control group seed germination rate x control group root length) x 100%; Escherichia coli and roundworm egg mortality were detected according to the standard of NY / T 525-2021; The above results are shown in Tables 2 and 3.

[0062] Table 2 Statistical results of microbial inoculant for sheep manure decomposition of each treatment group As shown in Table 2, after the sheep manure was decomposed, the organic matter content of Examples 1-3 was low, the decomposition was more thorough, the properties were stable, and the relative content of nitrogen, phosphorus and potassium was improved to a certain extent. In addition, the seed germination index of Examples 1-3 after decomposition was as high as more than 89%, indicating that the degree of decomposition of sheep manure was high and non-toxic; and the Escherichia coli in sheep manure decomposed by the compound microbial inoculant of Examples 1-3 was not detected, and the mortality of roundworm eggs was higher, indicating that the safety of the product after decomposition was also higher.

[0063] Table 3 Appearance and time to maximum temperature during sheep manure composting process of different treatments As shown in Table 3, the compound microbial inoculant of Examples 1-3 can effectively improve the composting temperature, decompose quickly, have no odor, and have the best decomposition effect. The decomposition effect of Comparative Examples 1-9 with different microbial compositions all decreased to different degrees, especially the decomposition effect of Comparative Examples 4-9 decreased significantly after the synergistic effect of the three strains disappeared.

[0064] It should be noted that the above-mentioned embodiments only illustrate some but not all of the preferred ways for implementing the present application. Obviously, based on the above-mentioned embodiments of the present application, all other embodiments obtained by a person of ordinary skill in the art without creative work should belong to the protection scope of the present application.

Claims

1. A compound microbial inoculant for the rapid composting of livestock and poultry manure, characterized in that, The raw materials include the following parts by weight: 30-40 parts of compound microbial fermentation broth, 40-50 parts of organic carrier and conditioner, 5-8 parts of nutritional aid, 3-5 parts of mineral element aid, and 2-4 parts of promoter.

2. The compound microbial agent for rapid composting of livestock and poultry manure according to claim 1, characterized in that, The compound microbial fermentation broth includes *Bacillus thermophilus*, *Heterospora thermosus*, and *Arthrobacter trophoblastus*; the *Bacillus thermophilus* (… Geobacillus thermoleovorans The strain number is CCTCC WB 20081274, and the thermostable heterospores ( Aliibacillus thermotolerans The strain numbered CGMCC 1.15790, described as *Arthrobacter trophozoites* (… Arthrobacter pascens The strain number is CGMCC 1.6310.

3. The compound microbial agent for rapid composting of livestock and poultry manure according to claim 1, characterized in that, The preparation method of the aforementioned composite microbial fermentation broth is as follows: Thermophilic Bacillus, thermostable Heterobacterium, and Arthrotrophic Bacillus were activated and cultured separately to obtain viable counts of 1×10⁻⁶ cells each. 9 The fermentation broth has a concentration of CFU / ml. The three fermentation broths are mixed in a volume ratio of 1:1:1 to obtain a composite microbial fermentation broth.

4. The compound microbial agent for rapid composting of livestock and poultry manure according to claim 1, characterized in that, The organic carrier and conditioner are bentonite and mushroom residue, with a mass ratio of (1-2):

1.

5. The compound microbial agent for rapid composting of livestock and poultry manure according to claim 1, characterized in that, The nutritional aids include molasses and soybean meal in a mass ratio of (4-6):

1.

6. The compound microbial agent for rapid composting of livestock and poultry manure according to claim 1, characterized in that, The mineral-containing additive is superphosphate.

7. The compound microbial agent for rapid composting of livestock and poultry manure according to claim 1, characterized in that, The accelerator is humic acid and ferrous sulfate, with a mass ratio of (2-4):

1.

8. A method for preparing a compound microbial inoculant for rapid composting of livestock and poultry manure as described in any one of claims 1-7, characterized in that, Includes the following steps: (1) First, prepare the compound microbial fermentation broth, then dilute the molasses with 50°C warm water, and then mix it evenly with the compound microbial fermentation broth to form a liquid mixture; (2) Put bentonite, mushroom residue, soybean meal, superphosphate, humic acid and ferrous sulfate into a mixer and stir for 30-45 minutes until all dry materials are fully mixed. (3) While the mixer is continuously stirring, the liquid mixture prepared in step (1) is evenly sprayed onto the dry material in step (2), and stirred until the liquid components are evenly adsorbed by the dry material. The mixture is then placed in a granulator for granulation to obtain solid particles with a particle size of 3-5 mm. The composite microbial agent is then dried at low temperature.

9. The application of the compound microbial agent for rapid composting of livestock and poultry manure as described in any one of claims 1-7, characterized in that, The livestock and poultry manure mentioned is sheep manure.

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