Detection device for bladder cancer metabonomics markers

Abstract

The present invention discloses a bladder cancer metabonomics marker detection apparatus, and relates to the technical field of marker detection, the bladder cancer metabonomics marker detection apparatus comprises a detection apparatus main body and a detection test tube, the bottom of the detection apparatus main body is fixedly connected with a base, the inclined surface of the base is provided with an operation screen, and the detection test tube is used for placing a pretreated sample solution; the detection device body is fixedly connected to the rear half part of the upper surface of the base, and a first sliding groove is formed in the upper surface of the base. Under the action of the displacement mechanism, the sample droppers are driven to move towards the interiors of the detection test tubes to the positions close to the bottoms of the detection test tubes, and at the moment, under the action of the liquid inlet assembly, a pretreated sample solution is injected into each detection test tube through the splitter plate and the sample droppers at the same time; the sample subpackaging efficiency of the same sample during detection is improved, and the efficiency of the whole detection operation can be improved.

Description

Technical Field

[0001] This invention relates to the field of biomarker detection technology, and specifically to a device for detecting metabolomics biomarkers in bladder cancer. Background Technology

[0002] The detection of metabolomics biomarkers for bladder cancer is mainly based on the analysis of metabolites in bodily fluids such as urine and blood. High-throughput technologies are used to identify tumor-related metabolic abnormalities, aiding in early diagnosis, staging, and prognostic assessment. Urine, in particular, is of great significance in early bladder cancer screening and prognostic assessment because it comes into direct contact with bladder tumors, contains metabolites released by the tumor, and provides systemic metabolic information. Furthermore, urine samples are relatively easy to obtain and are non-invasive.

[0003] The procedure for testing urine samples is usually as follows: First, the patient collects a midstream urine sample using a sterile container as required. After receiving the sample, the operator quickly pre-processes it, then aliquots the pre-processed sample into multiple test tubes, and finally pushes the aliquoted test tubes into the testing device for final testing.

[0004] When the same sample needs to be tested multiple times, it means that the operator needs to perform multiple operations to pipette the pretreated sample into multiple test tubes, while ensuring that the amount of sample solution in each test tube is the same. This sample solution dispensing process is time-consuming and labor-intensive. Moreover, during the sample dispensing process, if the sample drop rate is too fast or the droplet volume is too large, it will collide with the test tube wall or liquid surface when it falls into the test tube. The kinetic energy of the droplet is converted into surface energy, causing the sample solution to splash and adhere to the test tube wall. This not only affects the final amount of sample solution in the test tube, but also some metabolites will adhere to the test tube wall, affecting the accuracy of subsequent test results. Therefore, this application proposes a detection device for bladder cancer metabolomics biomarkers to solve the above problems. Summary of the Invention

[0005] The present invention provides a device for detecting metabolomics biomarkers in bladder cancer, thereby addressing the problems mentioned in the background art.

[0006] To solve the above-mentioned technical problems, the technical solution adopted by the present invention is as follows: A detection device for metabolomics biomarkers in bladder cancer includes a detection device body and test tubes. A base is fixedly connected to the bottom of the detection device body, and an operation screen is installed on the inclined surface of the base. The test tubes are used to hold pretreated sample solutions. The detection device body is fixedly connected to the rear half of the upper surface of the base. A groove is formed on the upper surface of the base, and a test tube rack is movably connected to the inner wall of the groove. The test tube rack has multiple neatly arranged slots.

[0007] The test tube is movably connected to the slot. The test tube rack and the test tube can slide along the slide from the front half to the rear half of the upper surface of the base. After entering the main body of the testing device, the test tube is analyzed and tested.

[0008] It also includes multiple sample droppers, with a flow divider plate fixedly connected to the top of each sample dropper. A valve is installed at the connection between each sample dropper and the flow divider plate. The number of sample droppers is the same as the number of slots in the same row. The flow divider plate is equipped with a displacement mechanism for driving the sample droppers into the test tube near the bottom.

[0009] The flow divider plate is equipped with a liquid inlet component that guides the pretreated sample solution into the flow divider plate. The sample solution is then diverted by the flow divider plate to multiple sample droppers, which are then introduced into the test tubes respectively.

[0010] A further improvement of the technical solution of the present invention is that: the displacement mechanism includes a movable mounting block and a fixed mounting block respectively movably connected to both ends of the diverter plate; a drive screw and a guide rod are connected to the side of the fixed mounting block near the movable mounting block; a connecting horizontal plate is connected to one end of the drive screw and the guide rod that passes through the movable mounting block; a telescopic rod is fixedly connected to the bottom of both the fixed mounting block and the connecting horizontal plate; a base plate is fixedly connected to the bottom of the telescopic rod; two sliding grooves are opened on the upper surface of the base; the base plate is movably connected to the sliding grooves; and the base plate can slide back and forth along the sliding grooves.

[0011] A further improvement of the technical solution of the present invention is that: both ends of the driving screw three are movably connected to the fixed mounting block and the connecting horizontal plate, both ends of the guide rod are fixedly connected to the fixed mounting block and the connecting horizontal plate, the driving screw three is threadedly connected to the movable mounting block, and the guide rod is movably connected to the movable mounting block.

[0012] An adjusting slider is movably connected to one side of the test tube rack. A limiting slider is fixedly connected to the bottom of the adjusting slider. A second sliding groove is provided on the upper surface of the base. The adjusting slider can slide along the second sliding groove through the limiting slider.

[0013] A further improvement of the technical solution of the present invention is that: the liquid inlet assembly includes a liquid inlet pipe fixedly connected to the upper surface of the diverter plate, one end of the liquid inlet pipe is connected to a liquid pump, the liquid pump is fixedly connected to the fixed mounting block, and the output end of the liquid pump is connected to a connecting pipe.

[0014] A sample slot is provided on one side of the base, and a sample storage tank is movably connected inside the sample slot. One end of the connecting pipe is movably connected to the top of the sample storage tank.

[0015] A further improvement of the technical solution of the present invention is that: both the movable mounting block and the fixed mounting block are fixedly connected with mounting columns, and the two ends of the diverter plate are provided with mounting grooves, which are movably connected to the mounting columns.

[0016] A further improvement of the technical solution of the present invention is that: a buckle is fixedly connected to the fixed mounting block, the buckle is movably connected to the connecting pipe, the inner diameter of the buckle is greater than or equal to the outer diameter of the connecting pipe, the buckle is made of elastic material, the top of the buckle is open, and the opening distance is smaller than the outer diameter of the connecting pipe.

[0017] A further improvement of the technical solution of the present invention is that: an annular plate is fixedly connected to the outer surface of the bottom end of the sample dropper, the outer diameter of the annular plate is not greater than the inner diameter of the test tube, and multiple defoaming needles are fixedly connected to the annular plate, the multiple defoaming needles having different lengths and being staggered.

[0018] A further improvement of the technical solution of the present invention is that: each of the mounting posts is fitted with a spring on the outer surface of the bottom of the diverter plate, and one end of the spring is fixedly connected to the movable mounting block and the fixed mounting block.

[0019] Both ends of the diversion plate are fixedly connected to a lever, and both the movable mounting block and the fixed mounting block are movably connected to a rotating lever. When the rotating lever rotates, the protrusion of the rotating lever will drive the lever to slide down along the mounting column, which will drive the sample dropper, the diversion plate and the defoaming needle to slide down. The defoaming needle will puncture the air bubbles in the sample solution. At this time, the spring is in a compressed state.

[0020] A further improvement of the technical solution of the present invention is that: when the rotating block rotates to the point where its protrusion separates from the paddle, the paddle rebounds under the action of the compression spring. The rebound of the spring will cause the paddle to return to its original position and will not leave the range of the mounting post.

[0021] A further improvement to the technical solution of the present invention is that a handle is fixedly connected to one side of the sample storage tank.

[0022] Due to the adoption of the above technical solution, the technical progress achieved by this invention compared to the prior art is as follows: 1. This invention provides a detection device for metabolomics markers in bladder cancer. When the test tube rack is moved to the outermost position, multiple test tubes are sequentially inserted into the slots on the test tube rack along the sample dropper arrangement direction. Then, the displacement mechanism drives the sample droppers and the flow divider to move above the position of the first row directly opposite the test tubes. Then, the displacement mechanism continues to drive the sample droppers to move into the test tubes to a position closer to the bottom of the test tubes. At this time, through the action of the liquid inlet component, the pretreated sample solution is simultaneously injected into each test tube through the flow divider and multiple sample droppers, which speeds up the sample dispensing efficiency when performing the same test on the same sample and also improves the efficiency of the entire detection operation.

[0023] 2. This invention provides a detection device for metabolomics biomarkers in bladder cancer. Because the bottom of the sample dropper is very close to the bottom of the test tube, and as the sample solution is injected into the test tube through the sample dropper, the liquid level rises with the increase of the injected sample volume, and the sample dropper also rises slowly under the action of the displacement mechanism, the distance between the bottom opening of the sample dropper and the liquid level is also small. Therefore, there is basically no impact splashing during the injection of the sample solution, thus avoiding the problem of sample solution residues on the inner wall of the test tube, which would cause the sample solution or related components to adhere to the inner wall of the test tube and affect the accuracy of the detection results.

[0024] 3. This invention provides a detection device for metabolomics markers in bladder cancer. By using an antifoaming needle, bubbles floating on the liquid surface can be punctured, thereby minimizing the possibility of related negative problems caused by bubbles. Attached Figure Description

[0025] Figure 1 This is a schematic diagram of the structure of the present invention; Figure 2 This is a schematic diagram of the structure of the upper surface of the base of the present invention; Figure 3 This is a structural schematic diagram of the working process of the present invention; Figure 4 This is a schematic diagram of the sample dropper and displacement mechanism of the present invention; Figure 5 This is a schematic diagram of the structure on the mounting block of the present invention; Figure 6 This is a schematic diagram of the structure on the movable mounting block of the present invention; Figure 7 This is a schematic cross-sectional view of the flow divider in this invention. Figure 8 This is a schematic diagram of the structure of the annular plate and defoaming needle of the present invention; Figure 9 This is a schematic diagram of the structure of the sample storage tank of the present invention; Figure 10 This is a schematic diagram of the test tube rack of the present invention; Figure 11 This is a schematic diagram of the structure of the present invention for increasing the distance between the adjusting slider and the slide groove; Figure 12 This is a schematic diagram of the adjusting slider of the present invention.

[0026] In the diagram: 1. Main body of the detection device; 2. Base; 3. Operation panel; 4. Test tube rack; 5. Test tube; 6. Sample tank; 7. Slide 1; 8. Adjusting slider; 9. Drive screw 1; 10. Limiting slider; 11. Slide 2; 12. Drive screw 2; 13. Sample dropper; 14. Diverter plate; 15. Movable mounting block; 16. Fixed mounting block; 17. Drive screw 3; 18. Guide rod; 19. Connecting horizontal plate; 20. Telescopic rod; 21. Base plate; 22. Slide 3; 23. Drive screw 4; 24. Liquid inlet pipe; 25. Liquid pump; 26. Connecting pipe; 27. Buckle; 28. Sample storage tank; 29. ​​Paddle; 30. Mounting groove; 31. Mounting column; 32. Spring; 33. Rotating paddle; 34. Annular plate; 35. Defoaming needle. Detailed Implementation

[0027] The present invention will be further described in detail below with reference to embodiments: Example

[0028] like Figure 1-12 As shown, this invention provides a detection device for metabolomics biomarkers in bladder cancer, including a detection device body 1 and a test tube 5. The test tube 5 is used to hold a pretreated sample solution. The sample solutions mentioned in this application are all urine samples. The detection device body 1 is prior art. A base 2 is fixedly connected to the bottom of the detection device body 1. An operation screen 3 is installed on the inclined surface of the base 2. The operation screen 3 is prior art. The detection device body 1 is fixedly connected to the rear half of the upper surface of the base 2. The front half of the upper surface of the base 2 is used for sample injection. A groove 7 is opened on the upper surface of the base 2. A test tube rack 4 is movably connected in the inner wall of the groove 7. The test tube rack 4 has multiple neatly arranged slots. The diameter of the slots is larger than the outer diameter of the test tube 5, which facilitates the insertion or removal of the test tube 5. The test tube rack 4 is divided into two layers. The bottom layer has a semi-circular groove that matches the bottom of the test tube 5, which serves to support the test tube 5.

[0029] The test tube 5 is movably connected to the slot. The test tube rack 4 and the test tube 5 can slide along the slide groove 7 from the front half to the rear half of the upper surface of the base 2. After entering the body of the testing device 1, the test tube 5 is analyzed and tested. The inner wall of the slide groove 7 is movably connected to the drive screw 9. The drive screw 9 passes through the test tube rack 4 and is threadedly connected to the test tube rack 4. The rotation of the drive screw 9 can be driven manually or by a motor. When the drive screw 9 rotates, it can drive the test tube rack 4 to move along the slide groove 7 toward or away from the interior of the testing device body 1. Other methods can also be used to drive the test tube rack 4 to reciprocate along the slide groove 7, including but not limited to cylinders, electric telescopic rods, gear drives, etc.

[0030] It also includes multiple sample droppers 13, with a flow divider 14 fixedly connected to the top of each sample dropper 13. The flow divider 14 has a cavity within the connection range of the multiple sample droppers 13. The sample droppers 13 are conical with a small bottom aperture. A valve is installed at the connection between each sample dropper 13 and the flow divider 14. The valve is a prior art device. In this application, an electromagnetic valve or other valve for controlling the flow of liquid can be selected. The number of sample droppers 13 is the same as the number of slots in the same row. The outer diameter of the sample dropper 13 is smaller than the aperture of the test tube 5. The flow divider 14 is provided with a displacement mechanism for driving the sample dropper 13 into the test tube 5 near the bottom.

[0031] The flow divider 14 is equipped with a liquid inlet assembly that introduces the pretreated sample solution into the flow divider 14. The sample solution is split by the flow divider 14 into multiple sample droppers 13, which are then introduced into the test tubes 5 respectively.

[0032] Initially, the sample dropper 13, the flow divider 14, and the liquid inlet assembly are positioned close to the main body 1 of the detection device under the action of the displacement mechanism. At this time, the sample dropper 13 and the flow divider 14 are in a relatively high position, which does not affect the movement of the test tube rack 4 and the test tubes 5. When the test tube rack 4 moves to the outermost position, multiple test tubes 5 are inserted into the slots on the test tube rack 4 in sequence along the arrangement direction of the sample dropper 13. Then, the displacement mechanism drives the sample dropper 13 and the flow divider 14 to move above the position of the first row directly opposite the test tubes 5. Then, the displacement mechanism continues to drive the sample dropper 13 to move into the interior of the test tube 5 to a position closer to the bottom of the test tube 5. At this time, through the action of the liquid inlet assembly, the pretreated sample solution is simultaneously injected into each test tube 5 through the flow divider 14 and multiple sample droppers 13. This not only improves operational efficiency, but also, because the bottom of the sample dropper 13 is very close to the bottom of the inner cavity of the test tube 5, and as the sample solution is injected into the test tube 5 through the sample dropper 13, the liquid level rises with the increase of the injected sample volume, and the sample dropper 13 also rises slowly under the action of the displacement mechanism. This results in a small distance between the bottom opening of the sample dropper 13 and the liquid surface. Therefore, there is basically no impact splashing during the injection of the sample solution, thus avoiding the problem of sample solution residues on the inner wall of the test tube 5, which could cause sample solution or related components to adhere to the inner wall of the test tube 5 and affect the accuracy of the test results. At the same time, the valves connected to the liquid inlet assembly and the sample dropper 13 can be used to control the liquid inlet volume per unit time, ensuring the consistency of the added amount when adding sample solution to multiple test tubes 5 at the same time.

[0033] When the number of test tubes 5 in the last row of slots is less than the number of slots, the valve on the sample dropper 13 corresponding to the empty slot can be closed.

[0034] Furthermore, the displacement mechanism includes a movable mounting block 15 and a fixed mounting block 16 respectively movably connected to both ends of the diversion plate 14. A drive screw 17 and a guide rod 18 are connected to the side of the fixed mounting block 16 closest to the movable mounting block 15. A connecting horizontal plate 19 is connected to one end of the drive screw 17 and guide rod 18 that passes through the movable mounting block 15. Telescopic rods 20 are fixedly connected to the bottoms of both the fixed mounting block 16 and the connecting horizontal plate 19. The telescopic rods 20 are existing technology and can be driven pneumatically or electrically. Their function is to drive the connecting horizontal plate 19 and the fixed mounting block 16 to move up and down, thereby causing the sample dropper 13 and the diversion plate 14 to move up and down together. This allows the sample dropper 13 to penetrate deeper into the test tube 5, reducing potential splashing during sample solution injection. After the sample solution injection is complete, the telescopic rods 20 can be used to... The moving sample dropper 13 moves upward to the outside of the test tube 5. At this time, the test tube rack 4, carrying the test tube 5, slides along the slide groove 7 into the interior of the main body 1 of the detection device for subsequent testing. The bottom of the telescopic rod 20 is fixedly connected to the base plate 21. Two slide grooves 22 are opened on the upper surface of the base 2. The base plate 21 is movably connected to the slide grooves 22. The base plate 21 can slide back and forth along the slide grooves 22. The inner wall of the slide grooves 22 is movably connected to the drive screw 23. The drive screw 23 is threadedly connected to the base plate 21. The rotation of the drive screw 23 can be driven manually or by a motor. When the drive screw 23 rotates, it can drive the base plate 21 to move towards or away from the main body 1 of the detection device along the slide grooves 22. Other methods can also be used to drive the base plate 21 to move back and forth along the slide grooves 22, including but not limited to cylinders, electric telescopic rods, gear drives, etc.

[0035] In the initial stage, the base plate 21 slides along the slide groove 22 until the multiple sample droppers 13 are aligned with the multiple test tubes 5 in the first row. The telescopic rod 20 moves the sample droppers 13 down to a position close to the bottom of the test tubes 5. After the sample solution is injected, the telescopic rod 20 moves the sample droppers 13 up to a position away from the test tubes 5. At this time, the base plate 21 continues to slide along the slide groove 22, so that the multiple sample droppers 13 move to a position aligned with the multiple test tubes 5 in the second row. Then, the sample injection operation continues, and the above operation is repeated until the sample solution is injected into the last row of test tubes 5. Then, the telescopic rod 20 moves the sample droppers 13 up to the outside of the test tubes 5. Then, the base plate 21 moves along the slide groove 22 to a position closest to the main body 1 of the detection device. At this time, the test tube rack 4 can move the test tubes 5 along the slide groove 7 into the interior of the main body 1 of the detection device for subsequent detection.

[0036] Furthermore, both ends of the drive screw 17 are movably connected to the fixed mounting block 16 and the connecting horizontal plate 19, and both ends of the guide rod 18 are fixedly connected to the fixed mounting block 16 and the connecting horizontal plate 19. The drive screw 17 is threadedly connected to the movable mounting block 15, and the guide rod 18 is movably connected to the movable mounting block 15. A motor or other equipment and related accessories for driving the guide rod 18 to rotate are fixedly connected to the connecting horizontal plate 19. When the drive screw 17 rotates, the distance between the movable mounting block 15 and the fixed mounting block 16 can be adjusted to accommodate the diverter plate 14 of different sizes.

[0037] An adjusting slider 8 is movably connected to one side of the test tube rack 4. A limiting slider 10 is fixedly connected to the bottom of the adjusting slider 8. A second groove 11 is provided on the upper surface of the base 2. The adjusting slider 8 can slide along the second groove 11 through the limiting slider 10. A second drive screw 12 is movably connected to the inner wall of the second groove 11. The second drive screw 12 is threadedly connected to the limiting slider 10. The rotation of the second drive screw 12 can be driven manually or by a motor. When the second drive screw 12 rotates, it can drive the limiting slider 10 to move along the second groove 11. Other methods can also be used to drive the limiting slider 10 to reciprocate along the second groove 11, including but not limited to cylinders, electric telescopic rods, gear drives, etc.

[0038] Both slide rail 7 and adjusting slider 8 near the operation screen 3 are equipped with detachable baffles to limit the sliding range of test tube rack 4 towards the operation screen 3. When test tube rack 4 needs to be replaced, first remove the baffles on slide rail 7 and adjusting slider 8 near the operation screen 3, then remove test tube rack 4. Adjusting slider 8 slides along slide rail 11 via limiting slider 10, increasing the distance between adjusting slider 8 and slide rail 7. Next, screw one side of the replaced test tube rack 4 into the drive screw 9 in slide rail 7, allowing the other side of test tube rack 4 to slide into adjusting slider 8. Finally, [the process is repeated in the original text]. Once the removed baffle is installed, the number of single-row slots increases, which in turn increases the number of single-row test tubes 5. The guide rod 18 is rotated to move the movable mounting block 15 away from the fixed mounting block 16. A suitable size diversion plate 14 is selected so that the number of sample droppers 13 installed on the diversion plate 14 corresponds one-to-one with the number of single-row test tubes 5 on the replaced test tube rack 4. Thus, test tube racks 4 of different sizes can be selected according to different needs. Through the above structural settings, the device can cope with the detection under different conditions, expanding the applicability and utilization rate of the device.

[0039] The adjustment slider 8 is moved after the original test tube rack 4 is removed. Similarly, the movable mounting block 15 is moved after the flow divider 14 is removed from the movable mounting block 15 and the fixed mounting block 16.

[0040] Furthermore, the liquid inlet assembly includes a liquid inlet pipe 24 fixedly connected to the upper surface of the diverter plate 14. One end of the liquid inlet pipe 24 is connected to a liquid pump 25, which is fixedly connected to the fixed mounting block 16. The output end of the liquid pump 25 is connected to a connecting pipe 26. The liquid inlet pipe 24 and the liquid pump 25, as well as the liquid pump 25 and the connecting pipe 26, are connected by plug-in, threaded, or other detachable connection methods. The connecting pipe 26 is flexible and long enough to ensure that it is not affected when moving together under the action of the displacement mechanism.

[0041] A sample slot 6 is provided on one side of the base 2. A sample storage tank 28 is movably connected inside the sample slot 6. A handle is fixedly connected to one side of the sample storage tank 28 to facilitate the removal of the sample storage tank 28. One end of the connecting pipe 26 is movably connected to the top of the sample storage tank 28. The connection can be made by plugging, threading or other detachable connection methods.

[0042] When the sample solution needs to be tested, the sample storage tank 28 containing the pretreated sample solution is first placed into the sample tank 6. Then, the displacement mechanism moves multiple sample droppers 13 to directly above the first row of multiple test tubes 5. Then, the connecting pipe 26 is connected to the top of the sample storage tank 28. The displacement mechanism moves to send the sample droppers 13 into the test tubes 5 near the bottom of the inner cavity of the test tubes 5. Then, the liquid pump 25 is started to inject the sample solution in the sample storage tank 28 into the test tubes 5 through the connecting pipe 26, the liquid inlet pipe 24, the diverter plate 14, and the sample droppers 13. After the first row of multiple test tubes 5 is injected, the liquid pump 25 stops working. Then, the displacement mechanism and the liquid inlet assembly work together until all the test tubes 5 are injected with sample solution.

[0043] Furthermore, mounting posts 31 are fixedly connected to both the movable mounting block 15 and the fixed mounting block 16, and mounting grooves 30 are provided at both ends of the diverter plate 14, which are movably connected to the mounting posts 31.

[0044] Firstly, the purpose of the sample dropper 13 and the flow divider 14 is to introduce the sample solution. Since the sample solutions from different patients must not be cross-contaminated, in this application, the sample dropper 13, the flow divider 14, the sample storage tank 28, the inlet pipe 24 and the liquid pump 25, the connecting pipe 26 and the liquid pump 25, and the connecting pipe 26 and the sample storage tank 28 are all connected by a connection method that is easy to disassemble and assemble. Therefore, when one sample is tested and another sample needs to be replaced, the corresponding sample dropper 13, the flow divider 14, the inlet pipe 24, the connecting pipe 26, and the sample storage tank 28 all need to be replaced. The replacement of the sample dropper 13 and the flow divider 14 is facilitated by the insertion of the mounting groove 30 and the mounting column 31.

[0045] The ease of replacing sample dropper 13 and flow divider 14 is not limited to scenarios where different samples are being tested. It is also convenient to replace flow divider 14 and sample dropper 13 with those that can be matched when different sizes of test tube rack 4 are used.

[0046] For the testing of different samples, this application may also adopt other methods to process them, including but not limited to disassembling the used accessories for thorough cleaning and disinfection and reuse, so as to improve the efficiency of the device and avoid the problem of resource waste caused by using accessories such as sample dropper 13, flow divider 14, liquid inlet tube 24, connecting tube 26, and sample storage tank 28 as consumables.

[0047] Furthermore, a buckle 27 is fixedly connected to the fixed mounting block 16. The buckle 27 is movably connected to the connecting tube 26. The inner diameter of the buckle 27 is greater than or equal to the outer diameter of the connecting tube 26. The buckle 27 is made of elastic material. The top of the buckle 27 is open, and the gap between the openings is smaller than the outer diameter of the connecting tube 26. When no testing operation is being performed, one end of the connecting tube 26 can be snapped into the buckle 27. This not only avoids the connecting tube 26 from getting tangled, but also makes it easy to quickly find the end of the connecting tube 26 and quickly connect it to the sample storage tank 28.

[0048] Furthermore, an annular plate 34 is fixedly connected to the outer surface of the bottom end of the sample dropper 13. The outer diameter of the annular plate 34 is no greater than the inner diameter of the test tube 5. Multiple defoaming needles 35 are fixedly connected to the annular plate 34. The multiple defoaming needles 35 have different lengths and are staggered. During the sample solution injection process, air is easily trapped, which will form bubbles. Bubbles will cause the sample to separate or mix unevenly, affecting the uniformity of metabolite distribution. In addition, the oxygen in the bubbles may promote the degradation of easily oxidized metabolites such as polyunsaturated fatty acids and vitamin C in urine samples, changing their concentration. All of the above situations will affect the accuracy of marker detection. Therefore, in this application, after the sample injection is completed, a step can be added to move the sample dropper 13 and the defoaming needles 35 fixedly connected to the bottom up and down by the action of the telescopic rod 20. Through the action of the defoaming needles 35, the bubbles floating on the liquid surface can be punctured, thereby minimizing the possibility of related negative problems caused by bubbles.

[0049] Furthermore, each mounting post 31 is fitted with a spring 32 on the outer surface of the bottom of the diversion plate 14, and one end of the spring 32 is fixedly connected to the movable mounting block 15 and the fixed mounting block 16.

[0050] Both ends of the diversion plate 14 are fixedly connected to the levers 29. The movable mounting block 15 and the fixed mounting block 16 are both movably connected to the rotating levers 33. The movable mounting block 15 and the fixed mounting block 16 are both equipped with motors or other equipment and related accessories to drive the rotating levers 33 to rotate. When the rotating levers 33 rotate, the protrusion of the rotating levers 33 will drive the levers 29 to slide down along the mounting column 31, and drive the sample dropper 13, the diversion plate 14 and the defoaming needles 35 to slide down. The defoaming needles 35 puncture the air bubbles in the sample solution. At this time, the spring 32 is in a compressed state. The length of some defoaming needles 35 is longer than the length from the annular plate 34 to the outlet end of the sample dropper 13. When the sample dropper 13 moves to the lowest injection point, some of the longer defoaming needles 35 pierce into the sample, which will cause a certain degree of disturbance to the sample, causing the air bubbles suspended in the middle of the sample solution to float up under the action of liquid disturbance. The tips of most of the defoaming needles 35 are flush with the liquid surface, which makes it easy to puncture the air bubbles floating on the liquid surface.

[0051] Although the telescopic rod 20 allows for defoaming after the sample solution is injected, bubbles may still be generated during the injection of the sample solution from the sample dropper 13 into the test tube 5. These bubbles may already affect the sample solution. Furthermore, the up-and-down movement of the telescopic rod 20 causes the movable mounting block 15, fixed mounting block 16, liquid pump 25, drive screw 17, guide rod 18, and other structures to move up and down together, resulting in unnecessary work and energy waste. Therefore, the defoaming can be mitigated by rotating the lever 33, which rotates the protruding part of the lever 33. When the plate 29 comes into contact, the rotating block 33 continues to rotate. The protrusion of the rotating block 33 will press the plate 29, causing the diverter plate 14 to slide down a certain distance along the mounting post 31. At the same time, the spring 32 is compressed. After the rotating block 33 rotates until it separates from the plate 29, the sample dropper 13 and the diverter plate 14 will bounce back to their original positions under the action of the compressed spring 32. The change in this distance is small, which allows the sample dropper 13 and the defoaming needle 35 to move up and down within a small range. However, it will not cause the sample solution to splash when the sample is added due to the large upward movement of the sample dropper 13.

[0052] Since the defoaming needle 35 cannot completely cover the entire liquid surface, the sample dropper 13 and the defoaming needle 35 can be moved up and down within a small range to continuously defoam the liquid surface. At the same time, when a bubble is punctured, the tension formed on the surface of the bubble disappears, which will push other unpunctured bubbles to other positions. During the next or subsequent downward movement of the sample dropper 13 and the defoaming needle 35, the possibility of being punctured increases. With the above structure, theoretically, the generated bubbles can be eliminated as cleanly as possible, eliminating the negative effects of bubbles on the sample solution.

[0053] Furthermore, when the rotating block 33 rotates until its protrusion separates from the paddle 29, the paddle 29 rebounds under the action of the compression spring 32. The rebound action of the spring 32 will cause the paddle 29 to return to its original position and will not disengage from the mounting post 31.

Claims

1. A detection device for metabolomics biomarkers in bladder cancer, comprising a detection device body (1) and a test tube (5), wherein a base (2) is fixedly connected to the bottom of the detection device body (1), an operation screen (3) is mounted on the inclined surface of the base (2), and a pretreated sample solution is placed in the test tube (5), characterized in that: The main body (1) of the detection device is fixedly connected to the rear half of the upper surface of the base (2). A sliding groove (7) is provided on the upper surface of the base (2). A test tube rack (4) is movably connected in the inner wall of the sliding groove (7). A number of neatly arranged slots are provided on the test tube rack (4). The test tube (5) is movably connected to the slot. The test tube rack (4) and the test tube (5) can slide along the slide groove (7) from the front half to the rear half of the upper surface of the base (2) and enter the body (1) of the detection device to analyze and detect the test tube (5). It also includes multiple sample droppers (13), with a flow divider plate (14) fixedly connected to the top of each sample dropper (13). A valve is installed at the connection between each sample dropper (13) and the flow divider plate (14). The number of sample droppers (13) is the same as the number of slots in the same row. The flow divider plate (14) is provided with a displacement mechanism for driving the sample droppers (13) into the test tube (5) near the bottom. The flow divider (14) is provided with a liquid inlet assembly that introduces the pretreated sample solution into the flow divider (14). The sample solution is diverted through the flow divider (14) to multiple sample droppers (13) and introduced into the test tubes (5) respectively.

2. The detection device for bladder cancer metabolomics biomarkers according to claim 1, characterized in that: The displacement mechanism includes a movable mounting block (15) and a fixed mounting block (16) that are movably connected to both ends of the diverter plate (14). The fixed mounting block (16) is connected to a drive screw (17) and a guide rod (18) on the side near the movable mounting block (15). The drive screw (17) and the guide rod (18) are connected to a connecting horizontal plate (19) at one end of the movable mounting block (15). The bottom of the fixed mounting block (16) and the connecting horizontal plate (19) are both fixedly connected to a telescopic rod (20). The bottom of the telescopic rod (20) is fixedly connected to a base plate (21). Two sliding grooves (22) are opened on the upper surface of the base (2). The base plate (21) is movably connected to the sliding grooves (22). The base plate (21) can slide back and forth along the sliding grooves (22).

3. The detection device for bladder cancer metabolomics biomarkers according to claim 2, characterized in that: Both ends of the drive screw three (17) are movably connected to the fixed mounting block (16) and the connecting horizontal plate (19). Both ends of the guide rod (18) are fixedly connected to the fixed mounting block (16) and the connecting horizontal plate (19). The drive screw three (17) is threadedly connected to the movable mounting block (15), and the guide rod (18) is movably connected to the movable mounting block (15). The test tube rack (4) is movably connected to one side of an adjusting slider (8), and a limiting slider (10) is fixedly connected to the bottom of the adjusting slider (8). A second groove (11) is provided on the upper surface of the base (2). The adjusting slider (8) can slide along the second groove (11) through the limiting slider (10).

4. The detection device for bladder cancer metabolomics biomarkers according to claim 1, characterized in that: The liquid inlet assembly includes a liquid inlet pipe (24) fixedly connected to the upper surface of the diverter plate (14), one end of the liquid inlet pipe (24) is connected to a liquid pump (25), the liquid pump (25) is fixedly connected to the fixed mounting block (16), and the output end of the liquid pump (25) is connected to a connecting pipe (26). A sample slot (6) is provided on one side of the base (2), and a sample storage tank (28) is movably connected inside the sample slot (6). One end of the connecting pipe (26) is movably connected to the top of the sample storage tank (28).

5. The detection device for bladder cancer metabolomics biomarkers according to claim 2, characterized in that: Mounting posts (31) are fixedly connected to both the movable mounting block (15) and the fixed mounting block (16). Mounting slots (30) are provided at both ends of the diversion plate (14), and the mounting slots (30) are movably connected to the mounting posts (31).

6. The detection device for bladder cancer metabolomics biomarkers according to claim 2, characterized in that: The fixed mounting block (16) is fixedly connected with a buckle (27), which is movably connected to the connecting pipe (26). The inner diameter of the buckle (27) is greater than or equal to the outer diameter of the connecting pipe (26). The buckle (27) is made of elastic material. The top of the buckle (27) is open, and the gap between the openings is smaller than the outer diameter of the connecting pipe (26).

7. The detection device for bladder cancer metabolomics biomarkers according to claim 1, characterized in that: A ring plate (34) is fixedly connected to the outer surface of the bottom end of the sample dropper (13). The outer diameter of the ring plate (34) is not greater than the inner diameter of the test tube (5). Multiple defoaming needles (35) are fixedly connected to the ring plate (34). The multiple defoaming needles (35) have different lengths and are staggered.

8. The detection device for bladder cancer metabolomics biomarkers according to claim 7, characterized in that: Each of the mounting posts (31) is fitted with a spring (32) on the outer surface of the bottom of the diversion plate (14), and one end of the spring (32) is fixedly connected to the movable mounting block (15) and the fixed mounting block (16); Both ends of the diversion plate (14) are fixedly connected with a lever (29). The movable mounting block (15) and the fixed mounting block (16) are movably connected with a rotating lever (33). When the rotating lever (33) rotates, the protrusion of the rotating lever (33) will drive the lever (29) to slide down along the mounting column (31), which will drive the sample dropper (13), the diversion plate (14) and the defoaming needle (35) to slide down. The defoaming needle (35) will puncture the air bubbles in the sample solution. At this time, the spring (32) is in a compressed state.

9. The detection device for bladder cancer metabolomics biomarkers according to claim 8, characterized in that: When the rotating block (33) rotates until its protrusion separates from the paddle (29), the paddle (29) rebounds under the action of the compression spring (32). The rebound action of the spring (32) will cause the paddle (29) to return to its original position and will not leave the range of the mounting post (31).

10. A detection device for bladder cancer metabolomics biomarkers according to claim 5, characterized in that: A handle is fixedly connected to one side of the sample storage tank (28).

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