Soothing and repairing composition containing plant exosome and application thereof

Abstract

The invention relates to the technical field of daily chemicals, in particular to a soothing and repairing composition containing plant exosomes and application of the soothing and repairing composition. The composition is prepared from centella asiatica exosome, beta-glucan, 5-fold ceramide and hydrogenated lecithin. According to the invention, the quinary ceramide and the hydrogenated lecithin are subjected to a reverse evaporation method to obtain the quinary ceramide coated with the nano-phospholipid, and various ceramides are synergistic, complementary and synergistic, so that the risk of recrystallization of the ceramide is reduced, the encapsulation efficiency is high, and the permeability is enhanced.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of daily chemical technology, in particular to a soothing and repairing composition containing plant exosomes and application thereof. BACKGROUND

[0002] Skin is the largest organ of the human body, which is both a barrier and a sensory organ, and is easily damaged by various external factors such as ultraviolet radiation, air pollution, mechanical damage, excessive cleaning, temperature and humidity changes, etc., thereby leading to impaired skin barrier function. After the barrier is damaged, the skin often shows increased water loss, increased sensitivity, erythema, pruritus, burning and dry desquamation, and other uncomfortable symptoms. In recent years, with the continuous increase of sensitive skin population, the development of mild, safe and soothing and repairing skin care products has become an important research direction in the field of cosmetics.

[0003] The current repairing skin care products are often represented by active ingredients such as ceramides, hyaluronic acid, beta-glucan, plant polysaccharides and asiaticoside extract, which can improve skin barrier function, increase stratum corneum water content and reduce inflammatory response to some extent. However, the existing technology still has the following shortcomings:

[0004] Single component or single mechanism: Most repairing compositions often rely on a single barrier component (such as ceramide or hyaluronic acid), which is difficult to meet the multiple needs of anti-inflammatory, antioxidant, barrier repair and soothing and calming.

[0005] Poor stability of ingredients: Some bioactive substances (such as plant extracts and polysaccharides) are easily degraded in the formula by temperature, pH, light and other factors, resulting in insufficient long-term stability.

[0006] Limited penetration and efficacy: hydrophilic ingredients are difficult to effectively penetrate the stratum corneum, and lipophilic ingredients have poor dispersibility in the system, resulting in low active utilization rate.

[0007] Insufficient application of exosomes: In recent years, studies have shown that plant-derived exosomes are rich in various small RNAs, proteins, polysaccharides and active metabolites, which can transport active molecules across the membrane by vesicle, and have the effects of regulating inflammatory response, promoting fibroblast proliferation and accelerating damage repair. However, the application of plant exosomes in cosmetics is still in its infancy, and related researches are mostly focused on separation and purification process, lacking of complex composition synergized with classic repairing ingredients.

[0008] In summary, the existing technology has not yet provided a multifunctional composition with barrier repair, soothing and calming, moisturizing and stable delivery. Especially in the use of plant exosomes, there is a lack of scientific compounding strategy with ceramide, hyaluronic acid, beta-glucan and other ingredients, which is difficult to form a stable and effective skin care system.

[0009] Therefore, it is urgent to develop an innovative composition containing plant exosomes and synergistically acting with various classic repair ingredients to solve the problems of poor stability, single efficacy and insufficient utilization in the prior art, so as to realize efficient soothing and repair of damaged skin barrier. SUMMARY

[0010] To solve the above technical problems, the present application provides a soothing and repairing composition containing plant exosomes, which comprises: Centella asiatica exosomes, beta-glucan, 5-fold ceramide and hydrogenated lecithin.

[0011] As an embodiment of the present application, the mass ratio of the Centella asiatica exosomes, beta-glucan, 5-fold ceramide and hydrogenated lecithin is (0.3-3):(0.01-0.06):(0.025-0.25):(0.125-1.25).

[0012] As an embodiment of the present application, the molecular weight of the beta-glucan is 1000-300000 Da.

[0013] As an embodiment of the present application, the 5-fold ceramide comprises: ceramide EOP, NS, NP, AS and AP.

[0014] As an embodiment of the present application, the mass ratio of the ceramide EOP, NS, NP, AS and AP is (10-20):(15-25):(30-40):(15-25):(10-20).

[0015] As an embodiment of the present application, the 5-fold ceramide is wrapped by nanoliposomes.

[0016] The present application provides a soothing and repairing serum, which comprises the above-mentioned composition and cosmetically acceptable carriers or adjuvants.

[0017] As an embodiment of the present application, the adjuvant comprises PEG / PPG-17 / 6 copolymer, dipropylene glycol and arginine.

[0018] As an embodiment of the present application, the composition is suitable for application in cosmetics for sensitive skin or post-medical art repair.

[0019] As an embodiment of the present application, the composition is applied in antioxidant skin care products.

[0020] By adopting the above technical solution, the present application has the following beneficial effects:

[0021] Centella asiatica exosomes

[0022] The snow is cold and named, its nature is cold, so named snow, pharmacopoeia records have heat-clearing and detoxifying, swelling and dampness. The effect.

[0023] The unique phospholipid layer structure of Centella asiatica exosome is safe and easy to absorb, with high bioavailability. Centella asiatica exosome plays an important role in self material metabolism, physiological function maintenance and immune defense. Centella asiatica exosome mainly mediates cell communication through three ways: exosome and target cell interact with each other in a paracrine manner, adhere to the target cell surface through receptor-ligand interaction, and then be endocytosed into the target cell or directly released into the target cell, thereby activating the target cell; exosome directly enters the target cell by endocytosis; exosome can directly contact target cells for membrane fusion, so that nucleic acids and proteins in exosome are non-selectively transferred to target cells, thereby causing target cell response.

[0024] Centella asiatica exosome is extracted from fresh whole plant of Centella asiatica, which is a kind of extracellular vesicle secreted by plant cells extracted by multiple purification separation technology, containing RNA, protein and active ingredients such as secondary metabolites of Centella asiatica, which can combine with receptor cells, participate in signal transduction, regulate related physiological functions, and has the effects of soothing, repairing, anti-inflammatory and antioxidant.

[0025] Beta-glucan: extracted from high-cold oats using green process, with molecular weight of about 1000-300000 Da, which has excellent bioavailability and biological activity, and has the following functions:

[0026] Stimulate the growth of immune system and fibroblasts, accelerate the movement of keratinocytes, promote wound healing, promote the synthesis of collagen and hyaluronic acid, and provide strong moisturizing effect.

[0027] Liposome-encapsulated 5 ceramides

[0028] Ceramide EOP: densely repair the natural sebum film of the skin, strengthen the defense function of the skin surface barrier. Form a sound skin protection function, reduce water evaporation and loss.

[0029] Ceramide NS: inhibits the secretion of active sebaceous glands, balances the water and oil of the skin, and enhances the self-protection function of the skin.

[0030] Ceramide NP: similar to the material structure of the stratum corneum of the skin, can quickly penetrate into the skin, and help the renewal of the natural protective layer of the skin.

[0031] Ceramide AS: can combine with water in the stratum corneum to form a network structure, which firmly locks water.

[0032] Ceramide AP: through skin scale shedding, helps keratin metabolism, normalizes the natural peeling process of the skin, and restores smooth and tender skin.

[0033] The application uses reverse evaporation method to obtain nano-phospholipid-encapsulated 5 ceramides by using 5 ceramides and hydrogenated lecithin, the various ceramides are synergistically complementary and efficient, the risk of ceramide recrystallization is reduced, the encapsulation rate is high, and the penetration performance is enhanced. BRIEF DESCRIPTION OF DRAWINGS

[0034] Figures 1 to 3 : Volunteer use of Example 3, 28-day effect comparison chart. DETAILED DESCRIPTION

[0035] The application will be further explained in conjunction with specific embodiments.

[0036] The application provides a soothing and repairing composition containing plant exosomes, which comprises: centella asiatica exosomes, beta-glucan, 5 ceramides, and hydrogenated lecithin.

[0037] The β-glucan was prepared as follows:

[0038] Step 1: Shell 100 parts of high-cold oat, sieve, and crush to D90 ≤ 300 µm (target fineness about 200-300 µm can improve the contact area but avoid excessive release of polyphenols affecting color).

[0039] Drying moisture control: raw material moisture content < 10% (beneficial to storage and pretreatment uniformity).

[0040] Step 2: Mix the powder with water to make a wet paste with a solid-liquid ratio of 1:10 (w / w), and then treat it under a pulsed electric field. The conditions are: electric field strength: 2.0 kV / cm, pulse width: 5 µs, pulse number / total treatment time: 2000 pulses, temperature rise control: the system temperature rises < 8 ℃ during the total treatment (controlled by jacket cooling).

[0041] Step 3: Transfer the PEF-treated wet paste into a constant-temperature stirred tank, and adjust the pH to 5.0-6.0 by adding lactic acid. At a temperature of 45 ± 2 ℃, add α-amylase: 0.01%, low-activity cellulase: 0.005%; enzyme reaction time: 3.0 hours; terminate the enzyme reaction: after enzyme treatment, raise the temperature to 75 ℃ for 10 minutes to inactivate the enzyme, and then cool to room temperature.

[0042] Step 4: Remove the residue by centrifugation: collect the supernatant;

[0043] Step 5: Use a cross-flow filtration system to cascade membrane filtration to accurately control the target molecular weight distribution of 1000-300000 Da.

[0044] Step 6: Concentration and desalting, concentration by low temperature vacuum (temperature < 45 °C, reduced pressure 50-200 mbar) to control solids at 10%.

[0045] Step 7: Freeze-drying: first freeze at -40 °C for 12-24 h, then gradually increase the temperature to 0 °C in a vacuum freeze-drier, total freeze-drying time 48 h, to obtain a loose powder.

[0046] The 5 ceramide, hydrogenated lecithin was prepared as follows:

[0047] 1) Oil phase preparation (solvent dissolution)

[0048] In a 250 mL jacketed flask, add ethyl acetate 5.0 mL, ethanol 5.0 mL, stir under nitrogen protection, bath temperature control 40-45 °C.

[0049] Add hydrogenated lecithin 1.20 g, 5 ceramides total 0.15 g, tocopherol 0.05 g in sequence; stir for 15-25 min until clear or slightly translucent;

[0050] 2) Internal phase preparation

[0051] Weigh deionized water 10.00 g, add glycerol 0.5 g, filter 0.45 μm, keep room temperature.

[0052] 3) W / O emulsification

[0053] • Place the oil phase on a magnetic stirrer, turn on the high-speed sheeter. Add the internal phase to the oil phase slowly in a dropwise manner at 8000-10000 rpm (dropwise time 5-8 min), while maintaining shearing for 2-3 min to form a uniform W / O emulsion (total emulsification time 8-12 min).

[0054] 4) Reverse evaporation to remove solvent, transfer the obtained W / O emulsion to a rotary evaporation flask, connect a high-efficiency condenser and a solvent recovery system. Set the bath temperature to 30-35 °C.

[0055] Stage 1: Reduce the pressure to ~300-400 mbar, rotate at 100-150 rpm for 5-10 min to remove most of the volatile components;

[0056] Stage 2: Gradually reduce the pressure to ~100-150 mbar and maintain for 10-20 min;

[0057] Stage 3: Rapidly reduce the pressure to 20-40 mbar and maintain for 2-3 min, then back to normal pressure for 1-2 min, repeat 3-5 times; that is.

[0058] The application will be described in detail below with specific examples.

[0059] The composition of the application is made into a repair serum, and the scheme is as follows:

[0060] Table 1

[0061]

[0062] Table 2

[0063]

[0064] Explanation:

[0065] Comparative Examples 1-3 are serums made by removing any component in Example 1 of the composition of the application;

[0066] Comparative Examples 4-6 are serums made by replacing the composition of the application with ingredients on the market that are very recognized for their anti-sensitivity or repair effects: bisabolol, panthenol, and purslane extract.

[0067] Comparative Example 7 is a repair serum of a large brand on the market that is currently popular and has good reputation;

[0068] Comparative Example 8 is a serum made by increasing the content of all components in the composition of the application to more than the highest value in the application.

[0069] Comparative Example 9 is a blank serum obtained by removing all components in the composition of the application.

[0070] In the above Examples 1-Comparative Example 9,

[0071] The β-glucan was prepared as follows:

[0072] Step 1: Shell 100 parts of high-cold oat, sieve, and crush to D90 ≤ 300 µm (target fineness about 200-300 µm can improve the contact area but avoid excessive release of polyphenols affecting color).

[0073] Drying moisture control: raw material moisture content < 10% (beneficial to storage and pretreatment uniformity).

[0074] Step 2: The powder is mixed with water to a wet paste, solid to liquid ratio 1:10 (w / w), followed by treatment under pulsed electric field. Conditions: electric field strength: 2.0 kV / cm, pulse width: 5 µs, number of pulses / total treatment time: 2000 pulses, temperature rise control: temperature of the system rises < 8 ℃ during the total treatment (controlled with a jacket cooling).

[0075] Step 3: The PEF treated wet paste is transferred to a thermostatic stirred tank, the pH is adjusted to 5.0-6.0 with lactic acid, at temperature: 45 ± 2 ℃, a-amylase: 0.01%, low activity cellulase: 0.005% are added; enzyme reaction time: 3.0 hours; terminate enzyme reaction: after enzyme treatment, the temperature is raised to 75 ℃ for 10 minutes to inactivate the enzyme, then cooled to room temperature.

[0076] Step 4: Residues are removed by centrifugation: the supernatant is collected;

[0077] Step 5: The cross-flow filtration system is used, the cascade membrane filtration is used to accurately control the target molecular weight distribution 1000-300000 Da.

[0078] Step 6: Concentration and desalination, by low-temperature vacuum concentration (temperature ≤ 45 ℃, reduced pressure 50-200 mbar) to control the solid content 10%.

[0079] Step 7: Freeze-drying: first frozen at -40 ℃ for 12-24 h, then gradually warmed to 0 ℃ in a vacuum freeze dryer, the total freeze-drying time is 48 h, to get loose powder.

[0080] The 5 ceramide, hydrogenated lecithin was prepared as follows:

[0081] 1) Oil phase preparation (solvent dissolution)

[0082] In a 250 mL jacketed flask, add ethyl acetate 5.0 mL, ethanol 5.0 mL, stir under nitrogen protection, bath temperature control 40-45 ℃.

[0083] Add hydrogenated lecithin 1.20 g, 5 ceramides 0.15 g (adjust the proportion according to the different embodiments described above) one by one, and tocopherol 0.05 g; stir for 15-25 min until clear or slightly transparent;

[0084] 2) Inner phase preparation

[0085] Weigh deionized water 10.00 g, add glycerol 0.5 g, filter 0.45 μm, keep room temperature.

[0086] 4) W / O emulsification

[0087] • Place oil phase on a magnetic stirrer with high speed shearing on. Slowly add internal phase to oil phase drop-wise at 8000-10000 rpm (drop-wise time 5-8 min) while maintaining shearing for 2-3 min to form a uniform W / O emulsion (total emulsification time 8-12 min).

[0088] 4) Reverse evaporation desolventization Transfer the resulting W / O emulsion to a rotary evaporation flask, connect a high efficiency condenser to a solvent recovery system. Set bath temperature at 30-35 °C.

[0089] Stage 1: Reduce pressure to ~300-400 mbar, rotate at 100-150 rpm for 5-10 min to remove most of the volatile components;

[0090] Stage 2: Gradually reduce pressure to ~100-150 mbar, maintain for 10-20 min;

[0091] Stage 3: Quickly reduce pressure to 20-40 mbar for 2-3 min, then back to atmospheric pressure for 1-2 min, repeat 3-5 times; done.

[0092] The preparation method of the serum is as follows:

[0093] S01 Quickly transfer the colloidal phase in the rotary evaporation flask prepared in 5 ceramide, hydrogenated lecithin to the reaction kettle, and slowly add the preheated main water phase 88.10 g (part of the formula table 1 component) at a temperature of 50-60 °C, stirring at low speed (300-600 rpm) while adding, continue to stir at 50-60 °C for 5-10 min after adding to complete the preliminary hydration;

[0094] S02 Weigh the ingredients 8 and 9 in the formula table into a dry container, stir and heat to 60-70 °C, and reserve after complete dissolution;

[0095] S03 Weigh the ingredients 10 and 11 in the formula table into the S02 container, stir and dissolve, and reserve after dissolution;

[0096] S04 Weigh part of the ingredients 1 and 2-7 in the formula table into the S03 container, stir and heat to 80-85 °C, homogenize the material to make it uniform, then add step 3, and stir uniformly after cooling;

[0097] S05 Reduce the temperature to below 40 °C, add S01 and the remaining ingredients, homogenize for 8-10 min until the material is mixed uniformly, and then the repair serum prepared from the composition of the application is obtained.

[0098] I. Efficacy verification

[0099] (I) Efficacy test

[0100] I. Test instrument:

[0101] 1. The present application uses Finland Delfin VapoMeter SWL5201 transdermal water loss measuring instrument, VapoMeter uses closed cavity measurement principle, so the measurement is not affected by indoor air flow. The measurement time is short, which ensures the minimization of blocking normal evaporation.

[0102] 2. Finland Delfin Antera 3D is used for photography, Antera 3D uses innovative optical method and complex mathematical algorithm to obtain three-dimensional image. In this way, data can be extracted from the image related to the three-dimensional shape of the skin, which will be able to quantify the treatment effect and monitor the change over time. For example, the texture, smoothness, and depth and width of fine lines and wrinkles of the skin are measured. With its multi-spectral analysis, Antera software can also measure pigmentation and redness, which will provide meaningful information about sun damage, striae, etc.

[0103] II. Experimental principle:

[0104] TEWL value, transepidermal water loss (Transepidermal water loss, TEWL) measures the change of water vapor pressure adjacent to the skin surface. The TEWL value represents the condition of water loss in the stratum corneum, which is an important standard for evaluating the barrier function of the stratum corneum. The lower the TEWL value of the skin, the better the barrier function of the skin, and vice versa. The value of TEWL of skin water loss can be used to evaluate the efficacy of moisturizing and repairing cosmetics, and can also be applied to allergic patch test, contact dermatitis, physical therapy, burn and monitoring of new tissue, and timely find out whether the protective function of the skin has been damaged. The unit of TEWL is: g / hm3

[0105] III. Test population:

[0106] 30 people in each group, a total of 9 groups, aged 18-60 years old volunteers meeting the experimental requirements.

[0107] IV. Test conditions:

[0108] In room temperature 21±1℃, relative humidity 50±10%, and real-time dynamic monitoring, the subjects sit for 30 minutes before testing the relevant data.

[0109] V. Experimental process:

[0110] The experiment is divided into 9 groups, the left face of the first to the third group uses the application embodiment 3, and the right face uses the comparative example 1-3; the left face of the fourth to the seventh group uses the application embodiment 1, and the right face uses the comparative example 4-7; the left face of the eighth group uses the application embodiment 3, and the right face uses the comparative example 8; the left face of the ninth group uses the application embodiment 2, and the right face uses the comparative example 9; twice a day in the morning and evening, rub on the face, continue to use for 4 weeks, record the TEWL value, then select a left and right cheek on each volunteer's face, take a picture with Antera 3D, record the average redness value RED, and archive.

[0111] Six, experimental results

[0112] Skin trans-epidermal water loss rate (TEWL), average redness value (RED) test

[0113] The test sets the TEWL of the subject measured at week 0 as TEWL0, and the TEWL at week 4 as TEWL4, the TEWL change value as △TEWL, △TEWL = TEWL4- TEWL0, △TEWL is negative, and the lower the value, the less the skin moisture loss after four weeks, that is, the better the skin barrier function repair, and the healthier the skin;

[0114] The RED at week 0 is set as RED0, and the RED at week 4 is set as RED4, the RED change value is △RED, △RED = RED4-RED0, △RED is negative, and the lower the value, the less the skin redness, that is, the less the inflammatory reaction of the basal skin, the more comfortable the skin, and the better the soothing and anti-allergic effect of the product.

[0115] Table 3 shows the average △TEWL value and △RED value of 30 people in each group, as follows:

[0116]

[0117] The experimental results show that:

[0118] From the data in the above table, it can be seen that:

[0119] The △TEWL value and the △RED value are embodiment 1> embodiment 2> embodiment 3, which shows that as the proportion of the composition of the application increases, the △TEWL and the △RED value will correspondingly decrease, that is, the skin moisture loss is less and less, which shows that the integrity of the skin barrier is better, that is, the repair effect of the serum is better; the skin redness is less and less, which shows that the inflammatory reaction of the skin is less and less, that is, the soothing effect of the product is better;

[0120] The values of △TEWL and △RED of Example 1 are less than the values of △TEWL and △RED of Comparative Example 4, Comparative Example 5 and Comparative Example 6, which indicates that the soothing and redness-reducing effects of the composition of the present application are weakened when the composition is replaced by the commonly used and effective anti-sensitivity ingredients and repair ingredients on the market.

[0121] The values of △TEWL and △RED of Example 3 are less than the values of △TEWL and △RED of Comparative Example 1, Comparative Example 2 and Comparative Example 3, which indicates that the soothing and redness-reducing effects of the composition of the present application are weakened when any component in the composition is removed.

[0122] The values of △TEWL and △RED of Example 3 are less than the values of △TEWL and △RED of Comparative Example 8, which indicates that the soothing and redness-reducing effects of the composition of the present application are weakened when the amount of the composition is increased, and the increase in the content of the components will cause skin intolerance, increase the burden on the skin, and the effect is much worse than the effect within the range of the composition of the present application.

[0123] The values of △TEWL and △RED of Example 1 are less than the values of △TEWL and △RED of Comparative Example 7, which indicates that the soothing and redness-reducing effects of the composition of the present application are better than those of the commercially available product.

[0124] The values of △TEWL and △RED of Example 2 are less than the values of △TEWL and △RED of Comparative Example 9, which indicates that the soothing and redness-reducing effects of the blank serum obtained by removing the composition of the present application are not ideal.

[0125] Test picture comparison

[0126] The facial redness images of a part of the volunteers using the composition of the present application before and after are compared, and details are shown in Figure 1 As can be seen from the figure, the soothing and redness-reducing effect of the composition of the present application is very excellent after 28 days of use in the volunteers with more serious facial redness.

[0127] (II) Safety test (human skin patch test)

[0128] 1. Number of test persons: 40 persons, 10 men and 30 women, aged 18-60.

[0129] 2. Health status: The subjects have healthy skin and no history of skin disease and allergy, and meet the voluntary selection criteria.

[0130] 3. Test method: select qualified spot tester, in closed spot test method, put about 0.020-02025g of the test product in the spot tester, and use special adhesive tape to cover on the back of the subject, 11 coating points for each subject, remove the test product after 24 hours, and observe the skin reaction at 0.5, 24 and 48 hours after removal, respectively, score according to the skin reaction grading standard in the Hygienic Standard for Cosmetics, calculate the average value, and record the results in Table 5.

[0131] Evaluation criteria are shown in Table 4 Human Skin Patch Test Standard Table

[0132] Degree of reaction Score scale Skin reaction - 0 Negative reaction ± 1 Suspect reaction: only weak erythema + 2 Weak positive reaction (erythema reaction): erythema, infiltration, edema, possibly papules ++ 3 Strong positive reaction (vesicle reaction): erythema, infiltration, edema, papules, vesicles; reaction can extend beyond the collection area +++ 4 Very strong positive reaction (coalescing vesicle reaction): marked erythema, severe infiltration, edema, coalescing vesicles; reaction can extend beyond the test area

[0133] Table 5 Skin patch test score results

[0134] Score scale Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Comparative Example 7 Comparative Example 8 Comparative Example 9 0.5（h） 0 0 0 0 0 0 1 0 0 1 1 0 24（h） 0 0 0 0 0 0 2 0 0 0 1 0 48（h） 0 0 0 0 0 0 2 0 0 0 2 0

[0135] From the above table, it can be seen that each component of the composition of the application is very safe and very friendly to the skin, and is mild and non-irritating.

[0136] Liposome preparation process used in the composition of the application for testing the stability of the final product

[0137] Experimental instruments: 101-1AB electric heating air drying oven, QC609B biological ultraviolet light yellowing resistance tester, QFH-080A high and low temperature (alternating) test box, refrigerator

[0138] Test product: ①, the repair serum ① made by Example 3 of the application

[0139] ②, 5 ceramides in Example 3 of the application are not wrapped with liposomes, but are simply dissolved in oil and put into the serum to make Comparative Example 10 repair serum ②.

[0140] 3. Experimental process: the repair serum made by Example 3 and Comparative Example 10 is placed in a 100ml container, and they are placed in the corresponding containers, respectively, accelerated test (40±2℃, -18±1℃) for 3 months, influence factor test (50±2℃) for 50 days, light test (illumination is 4500lx±500lx) for 10 days, freeze-thaw test (50±2℃ / -5±2℃) for 5 cycles, and the stability of the product is detected and observed; the test results are shown in Table 6 below.

[0141] Table 6

[0142]

[0143] The experimental data shows that: from the above table, the liposome encapsulation technology used in the composition of the present application can solve the solubility problem of ceramide, so that the multiple ceramides which are difficult to dissolve can exist stably in the system, and the problem of product stability is solved.

[0144] Finally, it should be noted that: the above examples are only used to illustrate the technical solutions of the present application, but not to limit them; although the present application has been described in detail with reference to the foregoing examples, those skilled in the art should understand that: it can still modify the technical solutions recorded in the foregoing examples, or make equivalent replacement for part or all of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present application.

Claims

1. A soothing repair composition comprising plant exosomes, characterized in that, The composition comprises: Centella asiatica exosome, beta-glucan, 5-fold ceramide, hydrogenated lecithin.

2. The plant exosome-containing soothing repair composition of claim 1, wherein, The mass ratio of the Centella asiatica exosome, beta-glucan, 5-fold ceramide and hydrogenated lecithin is (0.3-3):(0.01-0.06):(0.025-0.25):(0.125-1.25).

3. The plant exosome-containing soothing repair composition of claim 1, wherein, The beta-glucan has a molecular weight of 1000-300000 Da.

4. The plant exosome-containing soothing repair composition of claim 1, wherein, The 5-fold ceramide comprises: ceramide EOP, NS, NP, AS and AP.

5. Soothing repair composition containing plant exosomes according to any one of claims 1 to 4, characterized in that, The mass ratio of the ceramide EOP, NS, NP, AS and AP is: (10-20):(15-25):(30-40):(15-25):(10-20).

6. Soothing repair composition containing plant exosomes according to any one of claims 1 to 4, characterized in that, The 5-fold ceramide is wrapped by nano-liposome.

7. A soothing repair serum, characterized in that, The composition comprises: Centella asiatica exosome, beta-glucan, 5-fold ceramide, hydrogenated lecithin.

8. A soothing repair serum according to claim 7, characterized in that, The composition comprises: Centella asiatica exosome, beta-glucan, 5-fold ceramide, hydrogenated lecithin.

9. The composition according to any one of claims 1 to 6, characterized in that, The composition comprises: Centella asiatica exosome, beta-glucan, 5-fold ceramide, hydrogenated lecithin.

10. The composition according to any one of claims 1 to 6, characterized in that, The composition comprises: Centella asiatica exosome, beta-glucan, 5-fold ceramide, hydrogenated lecithin. The composition comprises: Centella asiatica exosome, beta-glucan, 5-fold ceramide, hydrogenated lecithin. The composition comprises: Centella asiatica exosome, beta-glucan, 5-fold ceramide, hydrogenated lecithin. The composition comprises: Centella asiatica exosome, beta-glucan, 5-fold ceramide, hydrogenated lecithin. The composition comprises: Centella asiatica exosome, beta-glucan, 5-fold ceramide, hydrogenated lecithin. The composition comprises: Centella asiatica exosome, beta-glucan, 5-fold ceramide, hydrogenated lecithin. The composition comprises: Centella asiatica exosome, beta-glucan, 5-fold ceramide, hydrogenated lecithin. The composition comprises: Centella asiatica exosome, beta-glucan, 5-fold ceramide, hydrogenated lecithin. The composition comprises: Centella asiatica exosome, beta-glucan, 5-fold ceramide, hydrogenated lecithin. The composition comprises: Centella asiatica exosome, beta-glucan, 5-fold ceramide, hydrogenated lecithin. The composition comprises: Centella asiatica exosome, beta-glucan, 5-fold ceramide, hydrogenated lecithin. The composition comprises: Centella asiatica exosome, beta-glucan, 5-fold ceramide, hydrogenated lecithin. The composition comprises: Centella asiatica exosome, beta-glucan, 5-fold ceramide, hydrogenated lecithin. The composition comprises: Cent

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