Compound herbal multifunctional health-preserving fragrant moxibustion and preparation method and application thereof

Abstract

The invention provides compound herbal multifunctional health-preserving fragrant moxibustion as well as a preparation method and application thereof, and relates to the technical field of traditional Chinese medicines. The multifunctional health-preserving incense comprises rose, honeysuckle, dried orange peel, chrysanthemum, sweet-scented osmanthus, radix angelicae, codonopsis pilosula, fructus forsythiae, tuber fleeceflower stem, apocynum venetum, mint, agilawood, sandalwood, frankincense, medlar, red dates, astragalus membranaceus, elecampane and benzoin. The radix astragali and the radix codonopsis serve as monarch drugs, the fructus lycii, the red dates, the roses, the sweet-scented osmanthus and the frankincense serve as ministerial drugs, the honeysuckle flowers, the fructus forsythiae, the chrysanthemum flowers, the dried orange peel, the radix aucklandiae, the radix angelicae, the mint, the tuber fleeceflower stems, the apocynum venetum and the agilawood serve as adjuvant drugs, and the sandalwood and the All the medicines are combined to achieve the effects of benefiting qi, nourishing blood, promoting blood circulation to remove blood stasis, calming the mind, soothing the nerves and assisting in relieving upper respiratory tract inflammation. According to the invention, a proper extraction process is selected, effective active ingredients are reserved and generated, the combustion odor is reduced, and meanwhile, the comprehensive effects of regulating qi and blood, relieving inflammation and relieving emotion are realized through the dual effects of meridian stimulation and drug effect volatilization.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of traditional Chinese medicine, in particular to a compound herbal multifunctional health-care moxa and a preparation method and application thereof. BACKGROUND

[0002] With the acceleration of modern life pace, the number of sub-healthy population is increasing, chronic fatigue, blood deficiency, decreased immunity and upper respiratory inflammation and other problems are increasingly common. Traditional Chinese medicine theory believes that blood disorders and unsmooth meridians are the root of many diseases, and tonifying qi and blood, promoting blood circulation to remove blood stasis and regulating visceral function are the key to improving health status. Traditional moxibustion therapy such as moxibustion has significant effects on warming meridians, dispelling cold, supporting yang and preventing loss of essence, but it has large smoke and irritating odor, which may cause respiratory discomfort, and the function of traditional moxibustion is relatively single, which is difficult to meet the comprehensive needs of modern people for health care, treatment and relaxation of body and mind.

[0003] At present, the moxa products on the market mostly use single wormwood or other herbal raw materials, which have limited efficacy and may produce irritating odor during combustion, affecting the use experience. In addition, the auxiliary treatment products for upper respiratory inflammation (such as chronic pharyngitis, rhinitis, etc.) are mainly oral or external spray.

[0004] Therefore, it is of important application value to develop a compound herbal moxa product with pleasant odor, tonifying qi and blood, promoting blood circulation to remove blood stasis, calming the mind and relieving upper respiratory inflammation. SUMMARY

[0005] The present application aims to provide a compound herbal multifunctional health-care moxa and a preparation method and application thereof, and the health-care moxa provided by the present application has the effects of tonifying qi and blood, promoting blood circulation to remove blood stasis, calming the mind and relieving upper respiratory inflammation.

[0006] In order to achieve the above-mentioned application purposes, the present application provides the following technical solutions: The present application provides a compound herbal multifunctional health-care moxa, which comprises the following raw materials by weight: rose 8-12 parts, honeysuckle 4-7 parts, dried tangerine or orange peel 5-10 parts, chrysanthemum 5-10 parts, osmanthus 3-5 parts, white peony root 5-10 parts, radix codonopsis 12-15 parts, forsythia 5-10 parts, moonseed 4-7 parts, roxburgh rose 3-5 parts, mint 3-5 parts, agarwood 3-5 parts, sandalwood 5-10 parts, frankincense 10-15 parts, medlar 4-6 parts, red date 10-15 parts, milkvetch 15-20 parts, costus 5-10 parts and styrax 1-3 parts.

[0007] The application further provides a preparation method of the compound herbal multifunctional health-care moxa, comprising: crushing and mixing rose, honeysuckle, dried tangerine or orange peel and chrysanthemum powder to obtain a moxa base; mixing osmanthus, baizhi, mint, agarwood, sandalwood and costus, inoculating a compound microbial inoculum for anaerobic fermentation to obtain a fermentation product; mixing scutellaria baicalensis, milkweed, goji, jujube and astragalus, and extracting by high-voltage pulsed electric field to obtain a high-voltage pulsed electric field extract; mixing forsythia, moonseed, apocynum venetum and styrax, and extracting by an ethanol solution under ultrasonic extraction to obtain an ethanol extract; and mixing the moxa base, the fermentation product, the high-voltage pulsed electric field extract and the ethanol extract to obtain the compound herbal multifunctional health-care moxa.

[0008] Preferably, the compound microbial inoculum is composed of Pichia fermentans and Lactobacillus rhamnosus.

[0009] More preferably, the number of Pichia fermentans in the compound microbial inoculum is (3-6) x 10 9 CFU / mL, and the number of Lactobacillus rhamnosus is (5-8) x 10 9 CFU / mL.

[0010] More preferably, the anaerobic fermentation is performed at a temperature of 28-34 DEG C, a pH value of 6.2-6.8 and for 24-36 h.

[0011] Preferably, the high-voltage pulsed electric field has an electric field intensity of 20-25 kV / cm, a pulse frequency of 100-200 Hz, a pulse width of 35-45 mu s, a temperature of 25-35 DEG C and a time of 2-4 min.

[0012] Preferably, the ethanol solution has a volume fraction of 65%-75%.

[0013] Preferably, the ultrasonic extraction is performed at a power of 400-500 W, a frequency of 25-30 kHz, a temperature of 50-60 DEG C, a number of times of 1-3 times and a time of 20-40 min.

[0014] The application further provides application of the compound herbal multifunctional health-care moxa in preparation of a product for tonifying qi and nourishing blood, promoting blood circulation to remove blood stasis and calming the mind.

[0015] The application further provides application of the compound herbal multifunctional health-care moxa in preparation of a product for assisting in relieving upper respiratory tract inflammation.

[0016] Compared with the prior art, the application has the following beneficial effects: The application provides a compound herbal multifunctional health-care incense, which comprises rose, honeysuckle, dried tangerine or orange peel, chrysanthemum, osmanthus, baizhi, radix codonopsis, forsythia, moonflower, apocynum venetum, mint, agarwood, sandalwood, frankincense, medlar, red date, radix astragali, costus and benzoin.

[0017] The application selects suitable extraction processes according to the characteristics of the herbs, retains and produces effective active ingredients, and reduces the burning odor. The incense has the characteristics of cool and sweet front notes, flower and honey fragrance middle notes, and lingering tail notes, so that the user is more likely to accept it. Meanwhile, through the double effects of meridian stimulation and drug efficacy volatilization, the comprehensive effects of qi and blood regulation, inflammation relief and mood relaxation are realized. The compound herbal multifunctional health-care incense is expected to become an upgraded substitute for traditional moxibustion therapy and meet the needs of modern people for green health care and convenient therapy. DETAILED DESCRIPTION

[0018] The application provides a compound herbal multifunctional health-care moxibustion, which comprises the following raw materials in parts by weight: rose 8-12 parts, honeysuckle 4-7 parts, dried tangerine or orange peel 5-10 parts, chrysanthemum 5-10 parts, osmanthus 3-5 parts, baizhi 5-10 parts, radix codonopsis 12-15 parts, forsythia 5-10 parts, moonflower 4-7 parts, apocynum venetum 3-5 parts, mint 3-5 parts, agarwood 3-5 parts, sandalwood 5-10 parts, frankincense 10-15 parts, medlar 4-6 parts, red date 10-15 parts, radix astragali 15-20 parts, costus 5-10 parts and benzoin 1-3 parts.

[0019] In the application, radix astragali and radix codonopsis are the monarch drugs for tonifying qi and blood, medlar, red date, rose, osmanthus and frankincense are the minister drugs for activating blood and resolving stasis, and the minister drugs assist the monarch drugs to enhance the blood-tonifying effect, honeysuckle, forsythia, chrysanthemum, dried tangerine or orange peel, costus, baizhi, mint, moonflower, apocynum venetum and agarwood are the auxiliary drugs for clearing heat and resolving toxins, treating upper respiratory tract inflammation, restraining the warm and dry nature of the tonifying drugs, regulating the spleen and stomach, nourishing the heart and calming the mind, and benzoin guides the nature of the drugs, harmonizes the drugs and enhances the fragrance. The application forms a formula system with multiple components, and the combination of the drugs has the effects of tonifying qi and blood, activating blood and resolving stasis, calming the mind, and assisting in relieving upper respiratory tract inflammation.

[0020] The present invention also includes excipients, which preferably include cassava starch, gum arabic, sodium carboxymethyl cellulose, glycerin, microcrystalline cellulose, and tourmaline powder. Cassava starch provides the main adhesive, ensuring the moxibustion strip maintains its structural integrity after extrusion and burns synergistically with the moxibustion matrix, maintaining stable smoldering. Gum arabic enhances the adhesiveness of cassava starch, improves plasticity during extrusion, and reduces the combustion rate. Glycerin, when used together, reduces irritating smoke during combustion. The combination of sodium carboxymethyl cellulose and microcrystalline cellulose forms a porous structure, promoting the release of active ingredients. Tourmaline powder releases far-infrared radiation during combustion, enhancing the transdermal absorption of the active ingredients.

[0021] This invention also provides a method for preparing the above-mentioned compound herbal multifunctional health-preserving aromatherapy moxibustion, comprising: pulverizing and mixing rose, honeysuckle, tangerine peel and chrysanthemum to obtain an aromatherapy moxibustion matrix; mixing osmanthus, angelica, mint, agarwood, sandalwood and costus root, inoculating with a compound bacterial agent for anaerobic fermentation to obtain a fermented product; mixing codonopsis, frankincense, wolfberry, red dates and astragalus, and extracting by a high-voltage pulsed electric field to obtain a high-voltage pulsed electric field extract; mixing forsythia, polygonum multiflorum, apocynum venetum and benzoin, and extracting by ultrasonic extraction with an ethanol solution to obtain an ethanol extract; and mixing the aromatherapy moxibustion matrix, fermented product, high-voltage pulsed electric field extract and ethanol extract to obtain the compound herbal multifunctional health-preserving aromatherapy moxibustion.

[0022] The composite microbial agent of this invention preferably consists of *Pichia fibiensis* and *Lactobacillus rhamnosus*. *Pichia fibiensis* is strain C1.8, purchased from the China Center for Type Culture Collection (CCTCCNO: M2017780), and disclosed in patent CN115466686A. *Lactobacillus rhamnosus* is strain RSF-1, purchased from the China General Microbiological Culture Collection Center (CGMCC NO. 23907), and disclosed in patent CN114231456A. ​​The preferred number of *Pichia fibiensis* in the composite microbial agent is (3-6) × 10⁻⁶. 9 CFU / mL, more preferably 5×10⁻⁶ 9 The optimal bacterial count of *Lactobacillus rhamnosus* is (5-8) × 10⁻⁶ CFU / mL. 9 CFU / mL, more preferably 7×10⁻⁶ 9The anaerobic fermentation temperature is preferably 28-34℃, more preferably 30℃, the pH value is preferably 6.2-6.8, more preferably 6.5, and the time is preferably 24-36h, more preferably 30h. This invention uses a compound microbial agent for anaerobic fermentation of aromatic raw materials such as osmanthus, angelica, peppermint, agarwood, sandalwood, and costus root. This process decomposes macromolecular components (such as cellulose and volatile oil precursors) in the medicinal materials, transforming them into more easily absorbed small molecules (such as flavonoid aglycones and organic acids). Simultaneously, it may enhance the stability of aromatic components, significantly increase the aroma content in the fermentation products, reduce combustion odors when burning the incense and moxibustion products, and achieve a cool and sweet initial note, a floral and honey middle note, and a lingering fragrance base note, making it more acceptable to users. It also has the effects of replenishing qi and blood, promoting blood circulation, removing blood stasis, calming the mind, and soothing the nerves.

[0023] The electric field strength of the high-voltage pulsed electric field described in this invention is preferably 20-25 kV / cm, more preferably 23 kV / cm; the pulse frequency is preferably 100-200 Hz, more preferably 150 Hz; the pulse width is preferably 35-45 μs, more preferably 40 μs; the temperature is preferably 25-35℃, more preferably 30℃; and the time is preferably 2-4 min, more preferably 3 min. This invention uses a high-voltage pulsed electric field to extract Codonopsis pilosula, frankincense, wolfberry, jujube, and Astragalus membranaceus. This method can destroy cell structures at low temperatures, efficiently retain heat-sensitive components (such as polysaccharides, saponins, and amino acids), avoid the destruction of beneficial components by high temperatures, and promote the enrichment of effective components.

[0024] The volume fraction of the ethanol solution described in this invention is preferably 65%-75%, more preferably 70%; the power of the ultrasonic extraction is preferably 400-500W, more preferably 450W; the frequency is preferably 25-30kHz, more preferably 28kHz; the temperature is preferably 50-60℃, more preferably 55℃; the number of extractions is preferably 1-3 times, more preferably 2 times; and the time is preferably 20-40min, more preferably 30min. This invention uses ethanol ultrasonic extraction to extract Forsythia suspensa, Polygonum multiflorum, Apocynum venetum, and Benzoin, which can effectively dissolve fat-soluble components (such as forsythosides, anthraquinones, volatile oils, etc.). Ultrasonic oscillation can accelerate cell disruption, improve extraction efficiency, and enrich components with sedative and anti-inflammatory effects.

[0025] This invention also provides an application of the above-mentioned compound herbal multifunctional health-preserving aromatherapy in the preparation of products that replenish qi and blood, promote blood circulation and remove blood stasis, and calm the mind and soothe the nerves.

[0026] The present invention also provides an application of the above-mentioned compound herbal multifunctional health-preserving aromatherapy in the preparation of products that help relieve upper respiratory tract inflammation.

[0027] The conventional method of using the incense moxibustion of this invention is as follows: The user lies down, and the practitioner first lights one end of the incense stick / stick, faces the user's back, holds the incense 2-3cm away from the user's skin, and the incense stick is at a 35-40 degree angle to the skin. The user's skin should feel a moderate warmth, and the practitioner performs clockwise spiral rotation moxibustion.

[0028] The health-preserving incense burner of this invention features a cool and sweet initial note, a floral and honey fragrance in the middle note, and a lingering fragrance in the final note when burned.

[0029] Precautions: This product is contraindicated for patients with severe illness, cancer, epilepsy, mental illness, cardiopulmonary disease, pregnant women, breastfeeding women, menstruating women, and those with allergies or adverse reactions. It is not for use by individuals under the age of 18. Keep this product out of reach of children. This product is not a substitute for medication or medical devices. Discontinue use if adverse reactions occur during use.

[0030] The technical solutions of this invention will be clearly and completely described below with reference to the embodiments thereof. Obviously, the described embodiments are only a part of the embodiments of this invention, and not all of them. All other embodiments obtained by those skilled in the art based on the embodiments of this invention without creative effort are within the scope of protection of this invention.

[0031] Unless otherwise specified, the following embodiments are all conventional methods.

[0032] Unless otherwise specified, all materials and reagents used in the following examples are commercially available.

[0033] Pichia ferbiensis C1.8 was purchased from the China Center for Type Culture Collection (CCTCCNO:M2017780) and is disclosed in patent CN115466686A; Lactobacillus rhamnosus strain RSF-1 was purchased from the China General Microbiological Culture Collection Center (CGMCC NO.23907) and is disclosed in patent CN114231456A.

[0034] Example 1 Preparation of Compound Herbal Multifunctional Health-Preserving Moxibustion (1) Weighing Accurately weigh the following ingredients according to their weight proportions: 10 parts rose petals, 6 parts honeysuckle, 8 parts dried tangerine peel, 8 parts chrysanthemum, 4 parts osmanthus, 8 parts angelica dahurica, 13 parts codonopsis pilosula, 8 parts forsythia suspensa, 6 parts polygonum multiflorum, 4 parts apocynum venetum, 4 parts mint, 4 parts agarwood, 8 parts sandalwood, 12 parts frankincense, 5 parts wolfberry, 12 parts red dates, 18 parts astragalus membranaceus, 8 parts costus root, and 2 parts benzoin.

[0035] (2) Preparation of fermentation products Osmanthus, Angelica dahurica, peppermint, agarwood, sandalwood and costus root were pulverized and passed through a 100-mesh sieve, then mixed to obtain mixture 1. Water was added to mixture 1 at 15 times its weight, and after sterilization, a compound bacterial agent was inoculated and anaerobic fermented at 30℃ and pH 6.5 for 30 hours. The mixture was then filtered, sterilized, concentrated and dried to a water content of 5% by weight to obtain the fermented product. The compound microbial agent consists of Pichia pastoris C1.8 and Lactobacillus rhamnosus strain RSF-1, with the Pichia pastoris C1.8 strain having a cell count of 5 × 10⁻⁶. 9 The CFU / mL count of Lactobacillus rhamnosus strain RSF-1 was 7 × 10⁻⁶. 9 CFU / mL, the inoculation amount of the compound microbial agent is 1% of the weight of mixture 1.

[0036] (3) Preparation of high voltage pulsed electric field extract Codonopsis pilosula, frankincense, wolfberry, red dates and astragalus were pulverized and passed through a 150-mesh sieve, then mixed to obtain mixture 2. Water with 10 times the weight of mixture 2 was added, and the mixture was extracted for 3 min at an electric field strength of 23 kV / cm, a pulse frequency of 150 Hz, a pulse width of 40 μs and a temperature of 30 ℃. The mixture was filtered, the filtrate was concentrated and dried to a water content of 3% by weight to obtain the high-voltage pulsed electric field extract.

[0037] (4) Preparation of ethanol extract Forsythia, Polygonum multiflorum, Apocynum venetum and Benzoin were pulverized and passed through a 100-mesh sieve, and mixed to obtain mixture 3. A 70% ethanol solution with a weight of 12 times that of mixture 3 was added, and the mixture was extracted twice at a power of 450W, a frequency of 28kHz and a temperature of 55℃ for 30 minutes each time. The filtrates were combined, concentrated and dried to a water content of 6% by weight to obtain the ethanol extract.

[0038] (5) Preparation of health-preserving incense moxibustion Rose petals, honeysuckle, dried tangerine peel, and chrysanthemum were pulverized separately, passed through a 60-mesh sieve, and mixed to obtain the aromatherapy base. This base was then mixed with fermentation products, high-voltage pulsed electric field extracts, and ethanol extracts to obtain mixture 4. Mixture 4 was then mixed with tapioca starch, gum arabic, sodium carboxymethyl cellulose, and glycerin at 200 rpm for 20 min. Microcrystalline cellulose and tourmaline powder were added, and the mixture was mixed at 10 rpm for 15 min. The mixture was then extruded, shaped, and dried to a moisture content of 3% by weight to obtain the health-preserving aromatherapy moxibustion. Glycerin was diluted with three times its volume of water before mixing. The weight ratio of mixture 4 to cassava starch, gum arabic, sodium carboxymethyl cellulose, glycerol, microcrystalline cellulose and tourmaline powder is 100:16:3.5:2.5:1.2:11:2.5.

[0039] Example 2 Preparation of Compound Herbal Multifunctional Health-Preserving Moxibustion (1) Weighing Accurately weigh the following ingredients according to their weight proportions: 8 parts rose petals, 4 parts honeysuckle, 5 parts dried tangerine peel, 5 parts chrysanthemum, 3 parts osmanthus, 5 parts angelica dahurica, 12 parts codonopsis pilosula, 5 parts forsythia suspensa, 4 parts polygonum multiflorum, 3 parts apocynum venetum, 3 parts mint, 3 parts agarwood, 5 parts sandalwood, 10 parts frankincense, 4 parts wolfberry, 10 parts red dates, 15 parts astragalus membranaceus, 5 parts costus root, and 1 part benzoin.

[0040] (2) Preparation of fermentation products Osmanthus, Angelica dahurica, peppermint, agarwood, sandalwood and costus root were pulverized and passed through an 80-mesh sieve, then mixed to obtain mixture 1. Water with a weight of 12 times that of mixture 1 was added, sterilized, and then inoculated with compound bacterial agent. Anaerobic fermentation was carried out at 28℃ and pH 6.2 for 36 hours. The mixture was filtered, sterilized, concentrated and dried to a water content of 4% by weight to obtain the fermented product. The compound microbial agent consists of Pichia pastoris C1.8 and Lactobacillus rhamnosus strain RSF-1, with the Pichia pastoris C1.8 strain having a cell count of 3 × 10⁻⁶. 9 The CFU / mL count of Lactobacillus rhamnosus strain RSF-1 was 5 × 10⁻⁶. 9 CFU / mL, the inoculation amount of the compound microbial agent is 2% of the weight of mixture 1.

[0041] (3) Preparation of high voltage pulsed electric field extract Codonopsis pilosula, frankincense, wolfberry, red dates and astragalus membranaceus were pulverized and passed through a 120-mesh sieve, and mixed to obtain mixture 2. Water with 8 times the weight of mixture 2 was added, and the mixture was extracted for 4 min at an electric field strength of 20 kV / cm, a pulse frequency of 100 Hz, a pulse width of 35 μs and a temperature of 25 ℃. The mixture was filtered, the filtrate was concentrated and dried to a water content of 4% by weight to obtain the high-voltage pulsed electric field extract.

[0042] (4) Preparation of ethanol extract Forsythia, Polygonum multiflorum, Apocynum venetum and Benzoin were pulverized and passed through an 80-mesh sieve, and mixed to obtain mixture 3. A 65% ethanol solution with a weight of 10 times that of mixture 3 was added, and the mixture was extracted 3 times at a power of 400W, a frequency of 25kHz and a temperature of 50℃, for 20 minutes each time. The filtrates were combined, concentrated and dried to a water content of 3% by weight to obtain the ethanol extract.

[0043] (5) Preparation of health-preserving incense moxibustion Rose petals, honeysuckle, dried tangerine peel, and chrysanthemum were pulverized separately, passed through a 40-mesh sieve, and mixed to obtain the aromatherapy base. This base was then mixed with fermented products, high-voltage pulsed electric field extracts, and ethanol extracts to obtain mixture 4. Mixture 4 was then mixed with tapioca starch, gum arabic, sodium carboxymethyl cellulose, and glycerin at 180 r / min for 22 min. Microcrystalline cellulose and tourmaline powder were added, and the mixture was mixed at 10 r / min for 15 min. The mixture was then extruded, shaped, and dried to a moisture content of 4% by weight to obtain the health-preserving aromatherapy moxibustion. Glycerin was diluted with three times its volume of water before mixing. The weight ratio of mixture 4 to cassava starch, gum arabic, sodium carboxymethyl cellulose, glycerol, microcrystalline cellulose and tourmaline powder is 100:15:3:2:1:10:2.

[0044] Example 3 Preparation of Compound Herbal Multifunctional Health-Preserving Moxibustion (1) Weighing Accurately weigh the following ingredients according to their weight proportions: 12 parts rose petals, 7 parts honeysuckle, 10 parts dried tangerine peel, 10 parts chrysanthemum, 5 parts osmanthus, 10 parts angelica dahurica, 15 parts codonopsis pilosula, 10 parts forsythia suspensa, 7 parts polygonum multiflorum, 5 parts apocynum venetum, 5 parts mint, 5 parts agarwood, 10 parts sandalwood, 15 parts frankincense, 6 parts wolfberry, 15 parts red dates, 20 parts astragalus membranaceus, 10 parts costus root, and 3 parts benzoin.

[0045] (2) Preparation of fermentation products Osmanthus, Angelica dahurica, peppermint, agarwood, sandalwood and costus root were pulverized and passed through a 120-mesh sieve, then mixed to obtain mixture 1. Water was added to mixture 1 at 18 times its weight, and after sterilization, a compound bacterial agent was inoculated and anaerobic fermented at 34℃ and pH 6.8 for 24 hours. The mixture was then filtered, sterilized, concentrated and dried to a water content of 4% by weight to obtain the fermented product. The compound microbial agent consists of Pichia pastoris C1.8 and Lactobacillus rhamnosus strain RSF-1. The number of Pichia pastoris C1.8 in the compound microbial agent is 6 × 10⁻⁶. 9 The CFU / mL count of Lactobacillus rhamnosus strain RSF-1 was 8 × 10⁻⁶. 9 CFU / mL, the inoculum amount of the compound microbial agent is 0.8% of the weight of mixture 1.

[0046] (3) Preparation of high voltage pulsed electric field extract Codonopsis pilosula, frankincense, wolfberry, red dates and astragalus membranaceus were pulverized and passed through an 180-mesh sieve, then mixed to obtain mixture 2. Water with a weight of 12 times that of mixture 2 was added, and the mixture was extracted for 2 min at an electric field strength of 25 kV / cm, a pulse frequency of 200 Hz, a pulse width of 45 μs and a temperature of 35 ℃. The mixture was filtered, the filtrate was concentrated and dried to a water content of 6% by weight to obtain the high-voltage pulsed electric field extract.

[0047] (4) Preparation of ethanol extract Forsythia, Polygonum multiflorum, Apocynum venetum and Benzoin were pulverized and passed through a 120-mesh sieve, and mixed to obtain mixture 3. An ethanol solution with a volume fraction of 75% and a weight of 15 times that of mixture 3 was added, and the mixture was extracted for 40 min at a power of 500W, a frequency of 30kHz and a temperature of 60℃. The mixture was filtered, the filtrate was concentrated and dried to a water content of 5% by weight to obtain the ethanol extract.

[0048] (5) Preparation of health-preserving incense moxibustion Rose petals, honeysuckle, dried tangerine peel, and chrysanthemum were pulverized separately, passed through an 80-mesh sieve, and mixed to obtain a moxibustion matrix. This matrix was then mixed with fermented products, high-voltage pulsed electric field extracts, and ethanol extracts to obtain mixture 4. Mixture 4 was then mixed with tapioca starch, gum arabic, sodium carboxymethyl cellulose, and glycerin at 250 rpm for 20 min. Microcrystalline cellulose and tourmaline powder were added, and the mixture was mixed at 10 rpm for 15 min. The mixture was then extruded, shaped, and dried to a moisture content of 4% by weight to obtain a health-preserving moxibustion product. Glycerin was diluted with three times its volume of water before mixing. The weight ratio of mixture 4 to cassava starch, gum arabic, sodium carboxymethyl cellulose, glycerol, microcrystalline cellulose and tourmaline powder is 100:18:4:3:1.5:12:3.

[0049] Comparative Example 1 The specific implementation method is the same as in Example 1, except that the compound microbial agent in the preparation of the ferment in step (2) is replaced with Pichia pastoris C1.8, with a cell count of 1.2 × 10⁻⁶. 10 The fermentation concentration was CFU / mL, the fermentation temperature was 28℃, the pH value was 6.5, and the fermentation time was 30h.

[0050] Comparative Example 2 The specific implementation method is the same as in Example 1, except that the compound bacterial agent in the preparation of the fermentation product in step (2) is replaced with Lactobacillus rhamnosus strain RSF-1, with a bacterial count of 1.2 × 10⁻⁶. 10 The fermentation concentration was CFU / mL, the fermentation temperature was 37℃, the pH value was 6.5, and the fermentation time was 30h.

[0051] Comparative Example 3 The specific implementation method is the same as in Example 1, except that the preparation of the high voltage pulse electric field extract in step (3) is replaced by: crushing Codonopsis pilosula, frankincense, wolfberry, red dates and Astragalus membranaceus into powder and passing them through a 150-mesh sieve, mixing them to obtain mixture 2, adding water at 10 times the weight of mixture 2, refluxing and extracting 3 times at 105°C for 40 minutes each time, filtering, combining the filtrates, concentrating the filtrate, and drying it to a water content of 3% by weight to obtain the water extract.

[0052] Comparative Example 4 The specific implementation method is the same as that in Example 1, except that the 70% volume fraction ethanol solution in step (4) of the preparation of the ethanol extract is replaced with water, and the ethanol extract is replaced with a water extract.

[0053] Comparative Example 5 The specific implementation method is the same as in Example 1, except that the preparation method of the health-preserving incense moxibustion is as follows: the weighed raw materials are crushed and passed through a 60-mesh sieve, and mixed to obtain mixture 5; the mixture is mixed with cassava starch, gum arabic, sodium carboxymethyl cellulose and glycerin at 200 r / min for 20 min, then microcrystalline cellulose and tourmaline powder are added, and the mixture is mixed at 10 r / min for 15 min, extruded and shaped, and dried to a water content of 3% by weight to obtain the health-preserving incense moxibustion; the weight ratio of mixture 5 to cassava starch, gum arabic, sodium carboxymethyl cellulose, glycerin, microcrystalline cellulose and tourmaline powder is 100:16:3.5:2.5:1.2:11:2.5.

[0054] Experimental Example 1 Sensory evaluation The health-preserving incense moxibustion prepared in Examples 1-3 and Comparative Examples 1-5 were selected as test samples and numbered as S1 (Example 1), S2 (Example 2), S3 (Example 3), D1 (Comparative Example 1), D2 (Comparative Example 2), D3 (Comparative Example 3), D4 (Comparative Example 4), and D5 (Comparative Example 5). Three moxibustion sticks of each type were randomly selected, and a 5cm section was cut from the middle as the test segment. They were placed in white porcelain dishes of the same size, numbered, and stored away from light.

[0055] Twenty members of the sensory evaluation team (10 men and 10 women, aged 25-35, all trained in aromatherapy sensory evaluation) were selected to evaluate the appearance, color, odor, burning state, and impurities of the compound herbal multifunctional aromatherapy moxibustion samples from Examples 1-3 and Comparative Examples 1-5. Each indicator was scored out of 20, 20, 30, 20, and 10 points respectively, for a total score of 100 points. A blind test method was used for the evaluation; each evaluator scored each indicator for each sample individually, and the average score of the 20 evaluators was taken as the final result. The sensory evaluation criteria are shown in Table 1, and the sensory evaluation results are shown in Table 2.

[0056] Table 1 Sensory Evaluation Criteria

[0057] Table 2 Sensory ratings of Examples 1-3 and Comparative Examples 1-5

[0058] As shown in Table 2, overall, the sensory evaluation scores of the incense moxibustion in the examples were higher than those in the comparative examples. The total scores of D1 and D2 were significantly lower than those of the examples, especially the score for the ignition aroma, indicating that the synergistic effect of the compound microbial agent was better for aroma and morphological stability. The scores for combustion state and color in D3 decreased, possibly due to the destruction of some components caused by high-temperature extraction, which affected combustion stability. The score for ignition aroma in D4 was lower than that of the examples, indicating that ethanol extraction was more conducive to the retention of volatile aroma components. All indicators in D5 were poor, indicating that the step-by-step extraction process (fermentation, electric field extraction, and ethanol extraction) was crucial to improving the overall sensory quality of the incense moxibustion.

[0059] As can be seen from the above, the compound herbal health-preserving moxibustion prepared in Examples 1-3 exhibits the best sensory quality, especially Example 1, which has the highest overall score. This indicates that its formula ratio and process parameters (compound microbial fermentation, high-pressure pulsed electric field extraction, and 70% ethanol extraction) are more suitable for the sensory requirements of health-preserving moxibustion. The synergistic fermentation of the compound microbial agent, the high-pressure pulsed electric field extraction technology, and the ethanol extraction process significantly improve the sensory indicators such as the appearance, aroma, and combustion performance of the moxibustion. Direct mixing of raw materials or single extraction / fermentation methods will lead to a decline in sensory quality. The health-preserving moxibustion of this invention has advantages such as regular shape, uniform color, rich aroma, stable combustion, and few impurities. Furthermore, when burning, it exhibits the characteristics of a cool and sweet initial note, a floral and honey middle note, and a lingering fragrance in the final note, meeting the sensory requirements of high-quality health-preserving moxibustion.

[0060] Experimental Example 2 Verification of Qi and Blood Nourishment Ninety SPF-grade female BALB / c mice (18-22g) were randomly divided into nine groups of ten mice each: blank control group, model control group, positive control group, Example 1 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, Comparative Example 4 group, and Comparative Example 5 group. Hulling conditions: SPF-grade environment, temperature 22±2℃, humidity 50±10%, 12h diurnal cycle, free access to food and water.

[0061] Except for the blank control group, mice in other groups were injected with cyclophosphamide at a concentration of 20 mg / mL at a dose of 80 mg / kg on day 1, and at a concentration of 20 mg / mL at a dose of 40 mg / kg on day 3. Starting from day 5, mice were forced to swim to exhaustion (water depth 30 cm, water temperature 25±1℃, with head submersion for 10 seconds as the criterion) once daily for 10 days (days 5-14). During the swimming period, the daily feed intake was reduced to 50% of the normal amount. Mice exhibiting dry, yellowing fur, huddling together, slow movement, and sluggish reactions, with swimming time reduced to less than 50% of the initial duration, indicated successful establishment of the qi and blood deficiency model. Examination confirmed that all mice successfully developed the model.

[0062] After successful modeling, starting on day 15, the positive control group was administered compound donkey-hide gelatin oral liquid by gavage at a dose of 10 mL / kg once daily. In Example 1 and Comparative Examples 1-5, mice were fixed using an adjustable-height fumigation frame, with the distance between the moxibustion and the mouse's nose adjusted to 10 cm. The moxibustion was burned for 20 minutes daily, once daily, for 14 consecutive days. The blank control group and the model control group received no treatment.

[0063] Fourteen days after intervention, mice were anesthetized and euthanized, blood was collected by enucleation, serum was separated, and the levels of hemoglobin (Hb), red blood cell count (RBC), serum ferritin (SF), and serum erythropoietin (EPO) were measured. The specific results are shown in Table 3.

[0064] Table 3 Effects of each group on Hb, RBC, SF and EPO levels

[0065] Table 3 shows that the levels of Hb, RBC, SF, and EPO in the model control group were significantly lower than those in the blank control group (p<0.01), indicating that the Qi and Blood Deficiency Model was successfully established. Although the indicators in the Example 1 group were slightly lower than those in the positive control group, they were significantly higher than those in the model control group, indicating that the moxibustion in Example 1 can improve Qi and Blood related indicators and has the effect of replenishing Qi and nourishing Blood. The levels of Hb, RBC, SF, and EPO in Comparative Examples 1-5 were lower than those in Example 1 group. This indicates that the Qi and Blood replenishing effects of the moxibustion in the comparative examples were weakened due to changes in the preparation process (such as single fermentation and the absence of high-voltage pulsed electric field extraction). This verifies that the preparation process of the moxibustion in Example 1 (compound microbial fermentation, high-voltage pulsed electric field extraction, etc.) is more advantageous in preserving and enhancing the Qi and Blood replenishing effects.

[0066] Experimental Example 3 Verification of its ability to help relieve upper respiratory tract inflammation Ninety SPF-grade male BALB / c mice (18-22g) were randomly divided into nine groups of ten mice each: blank control group, model control group, positive control group, Example 1 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, Comparative Example 4 group, and Comparative Example 5 group. Husbandry conditions: SPF-grade environment, temperature 22±2℃, humidity 50±10%, 12h diurnal cycle, free access to food and water.

[0067] Except for the blank control group, mice were anesthetized by intraperitoneal injection of 1% sodium pentobarbital at a dose of 40 mg / kg. LPS (lipopolysaccharide, 5 mg / mL, 10 μL / mouse) was then instilled into the nasal cavity using a micro-syringe for 3 consecutive days to induce upper respiratory tract inflammation. Mice exhibited symptoms such as nasal scratching, sneezing, and rapid breathing. Pathological examination revealed nasal mucosal congestion and inflammatory cell infiltration, indicating successful model establishment. Examination confirmed that the model was successfully established in all mice.

[0068] Starting on day 4 after modeling, the positive control group was administered dexamethasone by gavage at a dose of 1 mg / kg once daily. In Example 1 and Comparative Examples 1-5, mice were immobilized using an adjustable-height fumigation rack, with the distance between the incense and the mouse's nose adjusted to 10 cm. The incense was burned for 20 minutes daily for 7 consecutive days. The blank control group and the model control group received no treatment.

[0069] Seven days after intervention, mice were anesthetized and euthanized, blood was collected by enucleation, serum was separated, and the levels of IL-6, TNF-α and IL-1β in the serum of each group were detected by ELISA kit. The specific results are shown in Table 4.

[0070] Table 4. Effects of each group on the levels of inflammatory factors

[0071] Table 4 shows that the levels of inflammatory factors in the model control group were significantly higher than those in the blank control group, indicating that the upper respiratory tract inflammation model was successfully established. Compared with the model control group, all other groups showed relief of upper respiratory tract inflammation. The compound herbal health-preserving moxibustion prepared in Example 1 significantly reduced the levels of inflammatory factors in the upper respiratory tract inflammation model mice, indicating that the moxibustion in Example 1 can improve the symptoms of upper respiratory tract inflammation in mice. The levels of inflammatory factors in the Example 1 group were significantly lower than those in the comparative groups 1-5, indicating that the moxibustion prepared using the compound microbial agent fermentation, high-voltage pulsed electric field extraction, and ethanol extraction process is more effective in assisting the relief of upper respiratory tract inflammation, suggesting that the specific extraction process can enhance the anti-inflammatory activity of the moxibustion.

[0072] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A compound herbal multifunctional health-preserving aromatherapy moxibustion, characterized in that, The ingredients include the following parts by weight: 8-12 parts rose petals, 4-7 parts honeysuckle, 5-10 parts dried tangerine peel, 5-10 parts chrysanthemum, 3-5 parts osmanthus, 5-10 parts angelica dahurica, 12-15 parts codonopsis pilosula, 5-10 parts forsythia suspensa, 4-7 parts polygonum multiflorum, 3-5 parts apocynum venetum, 3-5 parts peppermint, 3-5 parts agarwood, 5-10 parts sandalwood, 10-15 parts frankincense, 4-6 parts wolfberry, 10-15 parts jujube, 15-20 parts astragalus membranaceus, 5-10 parts costus root, and 1-3 parts benzoin.

2. A method for preparing the compound herbal multifunctional health-preserving incense moxibustion according to claim 1, characterized in that, include: Rose, honeysuckle, dried tangerine peel and chrysanthemum are crushed and mixed to obtain the moxibustion matrix; osmanthus, angelica, mint, agarwood, sandalwood and costus are mixed and inoculated with compound bacteria for anaerobic fermentation to obtain the fermented product; codonopsis, frankincense, wolfberry, red dates and astragalus are mixed and extracted by high voltage pulse electric field to obtain high voltage pulse electric field extract. Forsythia, Polygonum multiflorum, Apocynum venetum and Benzoin were mixed and extracted with ethanol solution by ultrasound to obtain an ethanol extract; the moxibustion matrix, fermentation product, high voltage pulse electric field extract and ethanol extract were mixed to obtain compound herbal multifunctional health-preserving moxibustion.

3. The preparation method according to claim 2, characterized in that, The compound microbial agent consists of Pichia pastoris and Lactobacillus rhamnosus.

4. The preparation method according to claim 3, characterized in that, The number of *Pichia pastoris* cells in the compound microbial agent is (3-6) × 10⁻⁶. 9 CFU / mL, the bacterial count of Lactobacillus rhamnosus is (5-8)×10 9 CFU / mL.

5. The preparation method according to any one of claims 2-4, characterized in that, The anaerobic fermentation was carried out at a temperature of 28-34℃, a pH of 6.2-6.8, and a time of 24-36 hours.

6. The preparation method according to claim 2, characterized in that, The electric field strength of the high-voltage pulsed electric field is 20-25 kV / cm, the pulse frequency is 100-200 Hz, the pulse width is 35-45 μs, the temperature is 25-35℃, and the duration is 2-4 min.

7. The preparation method according to claim 2, characterized in that, The volume fraction of the ethanol solution is 65%-75%.

8. The preparation method according to claim 2, characterized in that, The ultrasonic extraction was performed at a power of 400-500W, a frequency of 25-30kHz, a temperature of 50-60℃, 1-3 times, and a time of 20-40 minutes.

9. The application of the compound herbal multifunctional health-preserving aromatherapy according to claim 1 in the preparation of products that replenish qi and blood, promote blood circulation and remove blood stasis, and calm the mind and soothe the nerves.

10. The application of the compound herbal multifunctional health-preserving aromatherapy according to claim 1 in the preparation of products that help relieve upper respiratory tract inflammation.

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