Double-optical detection device and detection method

Abstract

The invention relates to a dual-optical detection device and a detection method, and the dual-optical detection device comprises a light source device which is used for emitting exciting light; the function switching device can be switched between a fluorescence detection state and a chemiluminescence detection state; and the detection device is used for detecting fluorescence and chemiluminescence in the reaction liquid. And the zoom light receiving device is located between the function switching device and the detection device, and the zoom light receiving device can change the light receiving diameter of the zoom light receiving device. The double-optical detection device can perform fluorescence detection and chemiluminescence detection, so that required detection can be selected according to needs, the equipment cost is reduced, the equipment occupied space is reduced, and an inspector can perform rapid detection conveniently. The zoom light receiving device can effectively improve the accuracy of a detection result.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of in vitro diagnosis, in particular to a dual optical detection device and a detection method. BACKGROUND

[0002] Many detection items in the field of clinical detection analysis can be detected quickly by fluorescence detection technology and chemiluminescence detection technology. The detection principle of fluorescence detection technology is that antigen-antibody combination produces fluorescent substances. Under the action of special substrate liquid, the fluorescent substances radiate fluorescence, and under the action of a specific light path, the fluorescence can be quantitatively detected and analyzed, so as to realize the detection and analysis of target objects.

[0003] Chemiluminescence immunoassay is a new type of labeled immunoassay technology for detecting trace amounts of antigens or antibodies by combining chemiluminescence or bioluminescence system with immune reaction. Its detection principle is similar to radioimmunoassay and enzyme immunoassay, but it uses luminescent substances instead of radionuclides or enzymes as markers, and directly measures the luminescent intensity.

[0004] In specific applications, it is often necessary to select chemiluminescence immunoassay and fluorescence immunoassay for sample detection according to specific needs and occasions. The dual optical detection device in the prior art can perform fluorescence detection and chemiluminescence detection, but when the content of the to-be-detected substance is low, the user cannot accurately determine whether the to-be-detected substance is present, resulting in inaccurate detection results. SUMMARY

[0005] One object of the present application is to provide a dual optical detection device to at least solve one of the above technical problems.

[0006] To achieve the above object, the first aspect of the present application provides a dual optical detection device, comprising:

[0007] A light source device for emitting excitation light;

[0008] A function switching device capable of switching between a fluorescence detection state and a chemiluminescence detection state;

[0009] A detection device for detecting fluorescence and chemiluminescence in a reaction solution.

[0010] A zoom light collection device located between the function switching device and the detection device, the zoom light collection device being capable of changing its light collection diameter.

[0011] Optionally, the zoom light collection device can change its light collection diameter according to the light intensity of the fluorescence or the chemiluminescence; and / or the zoom light collection device can focus the fluorescence or the chemiluminescence to the detection device; or

[0012] The variable-focus light-receiving device adjusts its light-receiving diameter according to the type of luminescent liquid; or

[0013] The variable-focus light-receiving device adjusts its light-receiving diameter according to the fluorescent substance; or

[0014] The variable-focus light-receiving device is connected to the function switching device.

[0015] Optionally, when the light intensity of the fluorescence or the chemiluminescence exceeds a minimum preset value, the variable-focus light-receiving device increases its light-receiving diameter; and / or when the light intensity of the fluorescence or the chemiluminescence exceeds a maximum preset value, the variable-focus light-receiving device decreases its light-receiving diameter or

[0016] If the efficiency of the chemiluminescence generated by the luminescent liquid is high, the variable-focus light-receiving device decreases its light-receiving diameter; if the efficiency of the chemiluminescence generated by the luminescent liquid is low, the variable-focus light-receiving device increases its light-receiving diameter; or

[0017] If the fluorescence quantum yield of the fluorescent substance is high, the variable-focus light-receiving device decreases its light-receiving diameter; if the fluorescence quantum yield of the fluorescent substance is low, the variable-focus light-receiving device increases its light-receiving diameter.

[0018] Optionally, the variable-focus light-receiving device comprises a first housing, a plurality of lenses and a driving member, the plurality of lenses are arranged in the first housing and are arranged at intervals along the transmission direction of the fluorescence and the chemiluminescence, the number of the driving member is at least one, the driving member is connected with the lenses to adjust the interval of at least two adjacent lenses, and the plurality of lenses can focus the fluorescence and the chemiluminescence to the detection device.

[0019] Optionally, the plurality of lenses comprises at least one lens group, the lens group comprises a first convex lens, a first concave lens and a second convex lens arranged in sequence, wherein the first convex lens is arranged close to the detection device, the second convex lens is arranged close to the function switching device, and the interval between the first concave lens and the first convex lens and the second convex lens is adjustable.

[0020] Optionally, at least two lens groups are arranged, and in two adjacent lens groups, the second convex lens of one lens group is the first convex lens of the other lens group.

[0021] Optionally, the function switching device comprises at least two light channels and a working position.

[0022] Each of the light channels can be positioned at the working position, so that at least one of the light channels can transmit the chemiluminescence to the detection device, the function switching device is in the chemiluminescence detection state, and at least one of the light channels can transmit the excitation light of a preset wavelength to the reaction solution and transmit the fluorescence to the detection device, the function switching device is in the fluorescence detection state.

[0023] Optionally, at least one of the light channels comprises a filter and a dichroic mirror, the filter allows the excitation light of a preset wavelength to enter the light channel, and the dichroic mirror allows the fluorescence of a preset wavelength to pass through, so that the fluorescence enters the detection device.

[0024] Optionally, the function switching device comprises a second housing and a mounting frame, the mounting frame comprises the at least two light channels, and the mounting frame is movably arranged in the second housing, so that each of the light channels can be positioned at the working position, and the light channel positioned at the working position can transmit the excitation light of a preset wavelength and the fluorescence or can transmit the chemiluminescence.

[0025] Optionally, the mounting frame is rotatably arranged in the second housing, and the at least two light channels are arranged at intervals along the rotation center of the mounting frame; and / or

[0026] The second housing comprises a first light inlet, a first light inlet and outlet, and a first light outlet, the light channel comprises a second light inlet, a second light inlet and outlet, and a second light outlet, when the light channel is positioned at the working position, the first light inlet is opposite to the second light inlet, the first light inlet is opposite to the second light outlet, and the excitation light enters the second housing from the first light inlet, the excitation light of a preset wavelength exits the second housing from the first light inlet and outlet, and the fluorescence and the chemiluminescence enter the second housing from the first light inlet and outlet and exit the second housing from the first light outlet.

[0027] Optionally, the mounting frame comprises a frame body, the frame body is rotatably connected in the second housing, the light channel further comprises a light barrel, the light barrel is detachably connected to the frame body, and the at least one light barrel is provided with the filter and the dichroic mirror.

[0028] Optionally, the function switching device further comprises a first driving mechanism, the first driving mechanism is connected to the second housing and the mounting frame and drives the mounting frame to rotate.

[0029] Optionally, the second light outlet and the second light inlet and outlet are located on the same straight line, and the second light inlet and the second light outlet are arranged perpendicularly;

[0030] The filter is arranged at the second light inlet, and the dichroic mirror is arranged at the light channel and is at an angle of 45 degrees with the second light inlet and the second light inlet and outlet.

[0031] Optionally, the light source device comprises a light source and an incident optical fiber, the incident optical fiber being used for transmitting the excitation light emitted by the light source to the function switching device; and / or

[0032] The detection device comprises a detector and a receiving optical fiber, the receiving optical fiber being used for transmitting the fluorescence and the chemiluminescence output by the function switching device to the detector, and the detector being used for detecting the fluorescence and the chemiluminescence; and / or

[0033] The dual-optical detection device further comprises a second driving mechanism, the second driving mechanism being used for driving the detection box containing the reaction liquid to move in a three-dimensional space.

[0034] Optionally, the dual-optical detection device further comprises a complex aplanat focusing device, the complex aplanat focusing device being located downstream of the function switching device, and the complex aplanat focusing device being used for converging the light beams formed by the excitation light of different wavelengths on the same test site.

[0035] Another object of the present application is to provide a detection method to at least solve one of the above technical problems.

[0036] To achieve the above object, the second aspect of the present application adopts the following technical solution:

[0037] A detection method, the dual-optical detection device performing the detection method, the detection method comprising the following steps:

[0038] According to the detection mode required by the reaction liquid in the detection box, the function switching device is switched to the corresponding fluorescence detection state or chemiluminescence detection state.

[0039] The zoom light collecting device changes its light collecting diameter according to the light intensity of the detected fluorescence or chemiluminescence; or the zoom light collecting device changes its light collecting diameter according to the type of the luminescent liquid; or the zoom light collecting device changes its light collecting diameter according to the type of the fluorescent substance.

[0040] The reaction liquid is detected.

[0041] As can be seen, the technical scheme provided by the present application, when fluorescence detection is needed, the function switching device is switched to the fluorescence detection state, the light source device generates excitation light, the function switching device can transmit excitation light of a preset wavelength in the excitation light to the detection box, when the reaction liquid in the detection box contains a substance to be detected, the reaction liquid generates fluorescent substances, the fluorescent substances radiate and emit fluorescence under the action of the substrate liquid, the function switching device can transmit the fluorescence generated by the reaction liquid in the detection box to the detection device, so that the detection device detects the fluorescence, i.e., the fluorescence is quantitatively detected and analyzed, and fluorescence detection is realized.

[0042] When chemiluminescence detection is needed, the function switching device is switched to the chemiluminescence detection state, when the reaction liquid in the detection box contains a substance to be detected, the substance to be detected generates chemiluminescence, and the function switching device can transmit the chemiluminescence generated by the reaction liquid to the detection device, so that the detection device detects the chemiluminescence, the chemiluminescence is quantitatively detected and analyzed, and chemiluminescence detection is realized.

[0043] The zoom light collecting device can change its light collecting diameter, therefore, if the content of the substance to be detected is small and it cannot be determined whether the substance to be detected is contained, the zoom light collecting device can change its light collecting diameter to obtain a stronger light signal and determine whether the substance to be detected is contained. For example, if the zoom light collecting device increases the light collecting diameter and the detection device can obtain a stronger light signal corresponding to the substance to be detected, it can be determined that the substance to be detected is contained; if the zoom light collecting device increases the light collecting diameter and the detection device still cannot detect the light signal corresponding to the substance to be detected, it indicates that the reaction liquid does not contain the substance to be detected, or if the zoom light collecting device increases the light collecting diameter and the light signal still maintains the original intensity, it may be an interference signal and the reaction liquid does not contain the substance to be detected.

[0044] The double optical detection device can perform fluorescence detection and chemiluminescence detection, so that the tester can select the required detection according to needs, reduce the equipment cost and the occupied space of the equipment, facilitate the tester to perform rapid detection, and the zoom light collecting device can effectively improve the accuracy of the detection result, especially when the content of the substance to be detected is low. BRIEF DESCRIPTION OF DRAWINGS

[0045] Figure 1 is a structural schematic diagram of a double optical detection device provided by an embodiment of the present application;

[0046] Figure 2 is a structural schematic diagram of a part of a function switching device (remove a group of light channels) provided by an embodiment of the present application Figure 1 ;

[0047] Figure 3 is an exploded view of a function switching device (remove a group of light channels) provided by an embodiment of the present application;

[0048] Figure 4 is a sectional view of a function switching device provided by an embodiment of the present application;

[0049] Figure 5 is a schematic diagram of an internal light path of a dual optical detection device provided by an embodiment of the present application;

[0050] Figure 6 is a structural schematic of a partial function switching device (removing a set of light channels) provided by an embodiment of the present application Figure 2 ;

[0051] Figure 7 is Figure 3 a local enlarged view of A in FIG. 1;

[0052] Figure 8 is a structural schematic of a light barrel provided by an embodiment of the present application

[0053] Figure 9a is a structural schematic of an apochromatic focusing device and a detection box provided by an embodiment of the present application;

[0054] Figure 9b is a structural schematic of an apochromatic focusing device provided by an embodiment of the present application;

[0055] Figure 10 is a structural schematic of a zoom light collecting device provided by an embodiment of the present application;

[0056] Figure 11a is a schematic diagram of a plurality of lenses (a first concave lens is located at a first position) provided by an embodiment of the present application;

[0057] Figure 11b is a schematic diagram of a plurality of lenses (a first concave lens is located at a second position) provided by an embodiment of the present application;

[0058] Figure 11c is a schematic diagram of a plurality of lenses (a first concave lens is located at a third position) provided by an embodiment of the present application;

[0059] Figure 12 is a perspective schematic diagram of a zoom light collecting device provided by an embodiment of the present application.

[0060] in the figure:

[0061] 1, support;

[0062] 2, light source device; 21, light source; 22, incident optical fiber;

[0063] 3, function switching device;

[0064] 31, second housing; 311, first light inlet; 312, first light inlet and outlet; 313, first light outlet; 315, lower housing; 316, upper cover; 317, observation window; 318, sealing cover;

[0065] 32, mounting frame; 321, light channel; 3211, filter; 3212, dichroic mirror; 3213, second light inlet; 3234, second light inlet and outlet; 3235, second light outlet; 3236, light cylinder; 3237, limiting block; 322, frame body; 3221, limiting platform; 3222, shaft hole; 323, blocking piece; 324, bolt;

[0066] 33, first driving mechanism; 331, first driving part; 332, driven gear;

[0067] 34, fixed shaft;

[0068] 4, detection device; 41, detector; 42, receiving optical fiber;

[0069] 5, zoom light collecting device; 51, first housing; 52, lens; 521, lens group; 524, first convex lens; 525, first concave lens; 526, second convex lens; 53, driving part;

[0070] 6, doublet focusing device; 61, double convex lens; 62, concave lens; 63, plano-convex lens;

[0071] 7, excitation light; 8, fluorescence;

[0072] 10, detection box; DETAILED DESCRIPTION

[0073] The technical solutions of the present application will be further described below in conjunction with the accompanying drawings and through specific embodiments. It can be understood that the specific embodiments described herein are only used to explain the present application, but not to limit the present application. In addition, it should be noted that, for the convenience of description, only the parts related to the present application are shown in the drawings, but not all.

[0074] In the present application, some orientation words are defined. Without making the opposite statement, the orientation words used such as "upper", "lower", "left", "right", "inner", "outer" are used for the convenience of understanding, and thus do not constitute the limitation to the protection scope of the present application.

[0075] In the present application, unless otherwise explicitly specified and limited, the first feature is "on" or "under" the second feature can include that the first and second features are in direct contact, or can include that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, the first feature is "on", "above" and "over" the second feature includes that the first feature is directly above and obliquely above the second feature, or only indicates that the first feature is higher in horizontal height than the second feature. The first feature is "under", "below" and "underneath" the second feature includes that the first feature is directly below and obliquely below the second feature, or only indicates that the first feature is lower in horizontal height than the second feature.

[0076] In the description of the present application, unless otherwise explicitly specified and limited, the terms "connected", "connected", "fixed" should be understood broadly, for example, can be fixedly connected, or can be detachably connected, or integrated; can be mechanically connected, or electrically connected; can be directly connected, or indirectly connected through an intermediate medium, can be the internal communication of two elements or the interaction relationship between two elements. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0077] The embodiment provides a double optical detection device for fluorescence detection and chemiluminescence detection of exosomes such as blood, nasal swab and throat swab, so as to reduce equipment cost, reduce equipment space occupation and facilitate rapid detection of testers.

[0078] As shown in Figure 1 The double optical detection device provided by the embodiment includes a light source device 2, a function switching device 3, a zoom light receiving device 5 and a detection device 4. The light source device 2 is used for emitting excitation light 7, the function switching device 3 can be switched between a fluorescence detection state and a chemiluminescence detection state. The detection device 4 is used for detecting fluorescence 8 and chemiluminescence in the reaction solution. The zoom light receiving device 5 is located between the function switching device 3 and the detection device 4, and the zoom light receiving device 5 can change its light receiving diameter.

[0079] When the function switching device 3 is switched to the fluorescence detection state, the function switching device 3 can transmit the excitation light 7 of the preset wavelength in the excitation light 7 to the reaction solution of the detection box 10, and can transmit the fluorescence 8 generated by the reaction solution in the detection box 10 to the detection device 4. When the function switching device 3 is switched to the chemiluminescence detection state, the function switching device 3 can transmit the chemiluminescence generated by the chemiluminescence of the reaction solution to the detection device 4.

[0080] When fluorescence detection is needed, the function switching device 3 switches to the fluorescence detection state, the light source device 2 generates excitation light 7, and the function switching device 3 can transmit excitation light 7 of a preset wavelength in the excitation light 7 to the detection box 10. When the reaction solution in the detection box 10 contains the substance to be detected, the reaction solution emits fluorescence 8, and the function switching device 3 can transmit the fluorescence 8 generated by the reaction solution in the detection box 10 to the detection device 4, so that the detection device 4 detects the fluorescence 8, i.e. the fluorescence 8 is quantitatively detected and analyzed, and fluorescence detection is realized. For example, in the fluorescence detection technology, the substance to be detected is mixed with the fluorescent substance to form the reaction solution, the substance to be detected can be combined with the marker, and the marker can be combined with the fluorescent substance. When the excitation light of a specific wavelength irradiates the reaction solution, the fluorescent substance can absorb the excitation light and emit fluorescence 8 under the action of absorbing the excitation light, so that fluorescence detection can be realized. When chemical luminescence detection is needed, the function switching device 3 switches to the chemical luminescence detection state. When the reaction solution in the detection box 10 contains the substance to be detected, the substance to be detected generates chemical luminescence. The function switching device 3 can transmit the chemical luminescence generated by the reaction solution to the detection device 4, so that the detection device 4 detects the chemical luminescence. The chemical luminescence is quantitatively detected and analyzed, and chemical luminescence detection is realized. The zoom light collecting device 5 can change its light collecting diameter. Therefore, if the content of the substance to be detected is small and it cannot be determined whether the substance to be detected is contained, the zoom light collecting device 5 can change its light collecting diameter to obtain a stronger light signal and determine whether the substance to be detected is contained. For example, if the zoom light collecting device 5 increases the light collecting diameter, the detection device 4 can obtain a stronger light signal corresponding to the substance to be detected, so it can be determined that the substance to be detected is contained. If the zoom light collecting device 5 increases the light collecting diameter, the detection device 4 still cannot detect the light signal corresponding to the substance to be detected, which indicates that the reaction solution does not contain the substance to be detected. Or, if the zoom light collecting device 5 increases the light collecting diameter, the light signal still maintains the original intensity, which may be an interference signal, and the reaction solution does not contain the substance to be detected.

[0081] The double optical detection device provided in the embodiment can perform fluorescence detection and chemical luminescence detection, so that the tester can select the required detection according to the needs, reduce the equipment cost and the occupied space of the equipment, and facilitate the tester to perform rapid detection. At the same time, the zoom light collecting device 5 can effectively improve the accuracy of the detection result, especially when the content of the substance to be detected is low.

[0082] Optionally, the double optical detection device can further include a support 1, the light source device 2, the function switching device 3 and the detection device 4 are connected to the support 1. For example, the light source device 2, the function switching device 3 and the detection device 4 can be connected to the support 1 by welding, gluing, screw connection fastening or the like. The support 1 can carry the detection box 10 containing the reaction solution, so that the reaction solution in the detection box 10 can be detected.

[0083] In this embodiment, a reaction solution is formed by adding a substrate solution, a diluent, and other reagents to the exosomes. The reaction solution is then injected into the detection box 10, and the detection box 10 is placed on the support 1. The reaction solution can then be detected by fluorescence or chemiluminescence using a dual optical detection device.

[0084] Optionally, the light source device 2 may include a light source 21 and an incident optical fiber 22, the incident optical fiber 22 being used to transmit the excitation light 7 emitted by the light source 21 to the function switching device 3. For example, the light source 21 is full-spectrum light, thereby providing excitation light 7 of various wavelengths. One end of the incident optical fiber 22 may be connected to the light source 21, and the other end may be connected to the function switching device 3, thereby realizing the transmission of the excitation light 7.

[0085] For example, the detection device 4 may include a detector 41 and a receiving optical fiber 42. The receiving optical fiber 42 is used to transmit the fluorescence 8 and chemiluminescence output by the function switching device 3 to the detector 41, and the detector 41 is used to detect the fluorescence 8 and chemiluminescence. Optionally, one end of the receiving optical fiber 42 may be connected to the detector 41, and the other end may be connected to the function switching device 3, thereby realizing the transmission of fluorescence 8 and chemiluminescence. The detector 41 may include a silicon photomultiplier tube, a photonic detector 41, or a photomultiplier tube. The detector 41 using a silicon photomultiplier tube, a photonic detector, or a photomultiplier tube has high sensitivity, and during detection, it achieves ultrafast and high-sensitivity detection of optical signals at the millisecond level.

[0086] like Figures 2-5 As shown, the function switching device 3 may include at least two optical channels 321 and a working position. Each optical channel 321 can be located in the working position, so that at least one optical channel 321 can transmit chemiluminescence to the detection device 4, putting the function switching device 3 into a chemiluminescence detection state, and at least one optical channel 321 can transmit excitation light 7 of a preset wavelength to the reaction solution and fluorescence 8 to the detection device 4, putting the function switching device 3 into a fluorescence detection state. That is, at least one optical channel 321 can transmit excitation light 7 and fluorescence 8 of a preset wavelength. When the optical channel 321 that can transmit excitation light 7 and fluorescence 8 of a preset wavelength is located in the working position, the function switching device 3 is in a fluorescence detection state; at least one optical channel 321 can transmit chemiluminescence. When the optical channel 321 that can transmit chemiluminescence is located in the working position, the function switching device 3 is in a chemiluminescence detection state, thereby enabling the function switching device 3 to perform fluorescence detection and chemiluminescence detection.

[0087] Optionally, although the function switching device 3 can include at least two light channels 321, the same light channel 321 can perform both fluorescence detection and chemiluminescence detection, as long as the light channel 321 can transmit the chemiluminescence and fluorescence 8 and can transmit the excitation light 7. Of course, in other optional embodiments, the same light channel 321 can only perform fluorescence detection or only perform chemiluminescence detection. Whether the same light channel 321 can perform which detection is related to the wavelength of the light allowed to pass through the light channel 321. For example, the light channel 321 allows light with a wavelength of λ1 to pass through to transmit light with a wavelength of λ1 to the detection device 4. When the wavelengths of the chemiluminescence and fluorescence 8 are both λ1, the light channel 321 can perform fluorescence detection and chemiluminescence detection.

[0088] The detector 41 cannot identify the wavelength of the light. In fluorescence detection, the type of fluorescence 8 generated by the substance to be detected is specific, that is, the wavelength of the fluorescence 8 is specific. In order to make one light channel 321 only transmit fluorescence 8 of a predetermined wavelength outwards, so that the detector 41 can only detect the intensity of the fluorescence 8 generated by the substance to be detected. Optionally, at least one light channel 321 can include a filter 3211 and a dichroic mirror 3212. The filter 3211 allows the excitation light 7 of a predetermined wavelength to enter the light channel 321, and then the light channel 321 transmits the excitation light 7 of the predetermined wavelength to the reaction solution in the detection cartridge 10. The dichroic mirror 3212 allows the fluorescence 8 of a predetermined wavelength to pass through, so that the fluorescence 8 enters the detection device 4. At the same time, the dichroic mirror 3212 blocks the excitation light 7 reflected back by the reaction solution from passing through the dichroic mirror 3212, thereby avoiding the transmission of the excitation light 7 to the detection device 4, reducing noise and ensuring the accuracy of the detection result.

[0089] In this embodiment, a plurality of light channels 321 can be provided. Different light channels 321 transmit excitation light 7 of different wavelengths and / or fluorescence 8 of different wavelengths, so that the dual-optical detection device can detect different substances to be detected.

[0090] Optionally, at least one light channel 321 can transmit a predetermined wavelength of chemiluminescence to the detection device 4, so that the required chemiluminescence to be detected can also be transmitted to the detection device 4. Optionally, in at least one light channel 321, a dichroic mirror 3212 or a filter 3211 is provided on the path of chemiluminescence transmission, so as to only allow a predetermined wavelength of chemiluminescence to be transmitted to the detection device 4.

[0091] In other optional embodiments, at least one optical channel 321 can transmit chemiluminescence of all wavelengths to the detection device 4. When the function switching device 3 needs to switch to the chemiluminescence detection state, chemiluminescence detection is performed through the optical channel 321. For chemiluminescence detection, it is not necessary to excite the reaction liquid with excitation light 7. When the reaction liquid contains the analyte, it can generate self-luminescence of a specific wavelength under the action of the luminescent liquid, i.e., chemiluminescence. Therefore, the optical channel 321 only needs to transmit chemiluminescence. Thus, it is not necessary to filter the excitation light 7 with a dichroic mirror 3212, nor is it necessary to transmit the excitation light 7 with a preset wavelength with a filter 3211. Therefore, at least one optical channel 321 can transmit chemiluminescence of all wavelengths to the detection device 4.

[0092] In this embodiment, six optical channels 321 are provided, but the number of optical channels 321 is not limited to this; there may be more than six or less than six.

[0093] like Figures 2-3 As shown, exemplarily, the function switching device 3 may include a second housing 31 and a mounting bracket 32. Optionally, the working position is located within the second housing 31, and the mounting bracket 32 ​​may include at least two optical channels 321. The mounting bracket 32 ​​is movably disposed on the second housing 31 so that each optical channel 321 can be located in the working position. The optical channel 321 located in the working position can transmit fluorescence 8 and excitation light 7 of a preset wavelength or can transmit chemiluminescence. By moving the mounting bracket 32, the optical channels 321 are switched. Thus, when the optical channel 321 transmitting excitation light 7 of a preset wavelength and fluorescence 8 is located in the working position, the function switching device 3 is in fluorescence detection state; when the optical channel 321 transmitting chemiluminescence is located in the working position, the function switching device 3 is in chemiluminescence detection state.

[0094] Optionally, the mounting bracket 32 ​​is rotatably disposed within the second housing 31, and at least two optical channels 321 are spaced apart along the rotation center of the mounting bracket 32. The working position is located on the rotation path of the optical channel 321. When the mounting bracket 32 ​​rotates around the rotation center, each optical channel 321 can be located at the working position.

[0095] Of course, in other alternative embodiments, the optical channel 321 can also be positioned at and away from the working position by linear movement. For example, multiple optical channels 321 are arranged circumferentially. The multiple optical channels 321 can be located on regular patterns, such as on the same circle or polygons such as quadrilaterals or pentagons, or they can be located on irregular patterns. The working position is located inside the pattern formed by the multiple optical channels 321, such as the approximate center position of the pattern, so that each optical channel 321 is located at the working position and there is no interference between them when the optical channels 321 move.

[0096] likeFigure 4 As shown, optionally, the second shell 31 can include a first light inlet 311, a first light inlet-outlet 312 and a first light outlet 313, the light channel 321 can include a second light inlet 3213, a second light inlet-outlet 3234 and a second light outlet 3235, when the light channel 321 is in the working position, the first light inlet 311 is opposite to the second light inlet 3213, the first light inlet 311 is opposite to the second light outlet 3235, the excitation light 7 enters the second shell 31 through the first light inlet 311, the excitation light 7 of the preset wavelength is emitted from the second shell 31 through the first light inlet-outlet 312, the fluorescent light 8 and the chemiluminescence enter the second shell 31 through the first light inlet-outlet 312 and are emitted from the second shell 31 through the first light outlet 313, so as to realize the transmission of light.

[0097] As shown in Figure 1 and Figure 4 As shown, optionally, the incident optical fiber 22 is connected with the first light inlet 311, so that the excitation light 7 enters the second shell 31 and the light channel 321 through the first light inlet 311. The receiving optical fiber 42 is connected with the first light outlet 313, so that the fluorescent light 8 and the chemiluminescence in the light channel 321 enter the receiving optical fiber 42 through the first light outlet 313.

[0098] Optionally, the second light outlet 3235 and the second light inlet-outlet 3234 are located on the same straight line, and the second light inlet 3213 is vertically arranged with the second light outlet 3235. On the one hand, it can be convenient for light to enter the second shell 31 and the light channel 321, and for light in the second shell 31 and the light channel 321 to be emitted. It can be understood that the first light outlet 313 and the first light inlet-outlet 312 are also located on the same straight line, and the first light inlet 311 is also vertically arranged with the first light outlet 313.

[0099] As shown in Figure 4 and Figure 5 As shown, further, the optical filter 3211 is arranged at the second light inlet 3213, and the dichroic mirror 3212 is arranged in the light channel 321 and is at an angle of 45° with the second light inlet 3213 and the second light inlet-outlet 3234. After the excitation light 7 passes through the optical filter 3211, it is filtered into monochromatic excitation light 7 of the preset wavelength, and after the excitation light 7 of the preset wavelength is reflected by the dichroic mirror 3212, the transmission direction is turned by 90°, and then it can be transmitted to the second light inlet-outlet 3234. The fluorescent light 8 and the chemiluminescence enter the light channel 321 through the first light inlet-outlet 312 and the second light inlet-outlet 3234, and after straight-line transmission (passing through the dichroic mirror 3212), they are transmitted to the first light outlet 313 and the second light outlet 3235. At the same time, the excitation light 7 cannot pass through the dichroic mirror 3212 and is reflected by the dichroic mirror 3212, thereby avoiding noise pollution.

[0100] Optionally, asFigures 3-6 As shown in the figure, the mounting frame 32 can include a frame body 322 rotatably connected in the second housing 31, and the light channel 321 can further include a light cylinder 3236. In the embodiment, the second light outlet 3235, the second light inlet 3213 and the second inlet and outlet 3234 are all arranged on the light cylinder 3236. The light cylinder 3236 is detachably connected to the frame body 322, and at least one light cylinder 3236 is provided with a filter 3211 and a dichroic mirror 3212. Figure 2 、 Figure 3 、 Figure 6 and Figure 7 The filter 3211 and the dichroic mirror 3212 are omitted. When the required light channel 321 is not in the function switching device 3, the light cylinder 3236 with the required filter 3211 and dichroic mirror 3212 can be selected by replacing the light cylinder 3236, so as to provide the required light channel 321.

[0101] As shown in the figure, Figure 7 Optionally, the light cylinder 3236 can be a cuboid.

[0102] As shown in the figure, Figures 6-8 The light cylinder 3236 can be detachably connected to the frame body 322 by the connecting piece such as the bolt 324. As shown in the figure, Figure 7 and Figure 8 The light channel 321 can further include a limiting block 3237, and the outer side of the light cylinder 3236 is connected to the limiting block 3237. Specifically, the opposite sides of the light cylinder frame body 322 3236 are both provided with the limiting block 3237. The limiting block 3237 can be placed on the limiting table 3221 provided on the frame body 322.

[0103] The mounting frame 32 can further include a baffle 323, which can be detachably connected to the frame body 322 by the bolt 324. When the baffle 323 is connected to the frame body 322, the baffle 323 can stop the limiting block 3237, so as to avoid the limiting block 3237 from being separated from the limiting table 3221. The number of the baffles 323 can be one or more, as long as the limiting block 3237 can be fixed on the limiting table 3221. In the embodiment, two baffles 323 are provided corresponding to one limiting block 3237.

[0104] Of course, in other optional embodiments, the light cylinder 3236 can be detachably connected to the frame body 322 by clamping, such as connecting a clamping convex on the light cylinder 3236, and arranging a clamping groove on the frame body 322, which can be clamped with the clamping convex.

[0105] As shown in the figure, Figures 2-4 The function switching device 3 can further include a first driving mechanism 33 connected to the second housing 31 and the mounting frame 32, and driving the mounting frame 32 to rotate.

[0106] Exemplarily, the first driving mechanism 33 can include a first driving part 331, a driving gear (not shown in the figure) and a driven gear 332. The first driving part 331 is connected to the second shell 31, and can be a stepper motor or a motor, etc. The driving gear is connected to the output end of the first driving part 331, and the driven gear 332 is engaged with the driving gear. The first driving part 331 drives the driving gear to rotate, so that the driving gear drives the driven gear 332 to rotate. The mounting frame 32 is connected with the driven gear 332, so as to rotate with the driven gear 332. Exemplarily, the frame body 322 is connected with the driven gear 332.

[0107] As shown in Figure 4 , optionally, the mounting frame 32 is provided with an axle hole 3222, and specifically, the axle hole 3222 is provided in the frame body 322. As shown in Figure 3 , the second shell 31 is connected with a fixed shaft 34, the fixed shaft 34 penetrates the axle hole 3222, and a rolling bearing is arranged between the axle hole 3222 and the fixed shaft 34. When the mounting frame 32 rotates, the mounting frame 32 rotates around the fixed shaft 34. The fixed shaft 34 can improve the stability of the rotation of the mounting frame 32, thereby improving the detection accuracy.

[0108] As shown in Figure 3 , optionally, the second shell 31 can be provided with an observation window 317, and a sealing cover 318 is detachably connected to the observation window 317. When it is needed to check whether a failure occurs in the second shell 31, the sealing cover 318 can be opened, and after checking, the sealing cover 318 can be covered, so as to avoid dust from entering the second shell 31.

[0109] The second shell 31 can include a lower shell 315 and an upper cover 316, and the upper cover 316 covers the lower shell 315. The fixed shaft 34 can be connected to the second shell 31, and the mounting frame 32 can be accommodated in the lower shell 315.

[0110] Exemplarily, the first light inlet 311 and the first light inlet and outlet 312 are provided on the lower shell 315, and the first light outlet 313 is provided on the upper cover 316.

[0111] In the actual detection process, the user selects the appropriate luminescent liquid or fluorescent substance according to different detection targets, and the wavelength range of the chemical luminescence or fluorescence 8 generated by different types of reagents is also different. After the user determines the detection object, the luminescent liquid or fluorescent substance can be determined, the wavelength of the fluorescence 8 or the chemical luminescence is determined, and then the function switching device 3 can retrieve the corresponding light channel 321 according to the wavelength range selected by the user, so that the excitation light 7 with the best wavelength is used to irradiate the experimental light into the reaction liquid, and the light filter 3211 and the dichroic mirror 3212 are used to select and control the best excitation light 7, while avoiding the interference of stray light.

[0112] As Figure 1 , Figure 9a and Figure 9b shown, optionally, the dual optical detection device can further comprise a doublet focusing device 6, which is located between the function switching device 3 and the detection box 10, and is connected to the function switching device 3, for example, the doublet focusing device 6 is connected to the first light inlet and outlet port 312 of the second housing 31, and the light inlet end of the doublet focusing device 6 communicates with the first light inlet and outlet port 312. The detection box 10 can be located on the lower side of the doublet focusing device 6.

[0113] The doublet focusing device 6 is used to focus the excitation light 7 to the reaction liquid, so that when different optical channels 321 are switched, the light beams formed by excitation light 7 of different wavelengths can all be gathered on the same test site (as Figure 9b shown, three lines represent three light beams of different wavelengths), ensuring that the chromatic aberration, astigmatism, field curvature and other indicators between the reaction liquids are minimized, so as to ensure that the test environment does not change after the function switching device 3 switches different functions, realizing the mutual compatibility between different test systems, providing a hardware basis for the repeatability of the dual optical detection device, and further improving the detection precision. Exemplarily, the same test site specifically refers to the test point distance from the liquid level of the reaction liquid being consistent.

[0114] Chromatic aberration refers to that different wavelengths of light are gathered at different distances after passing through a common convex lens. The longer the wavelength, the greater the focal length, and the shorter the wavelength, the shorter the focal length. As Figure 9b shown, the doublet focusing device 6 can focus light of three wavelengths (red, green, and blue) on a plane, so that after passing through the doublet focusing device 6, the light beams formed by excitation light 7 of different wavelengths can all be gathered on the same test site.

[0115] Exemplarily, the doublet focusing device 6 comprises a double convex lens 61, a concave lens 62 and a plano-convex lens 63 arranged in sequence away from the function switching device 3, wherein the convex surface of the plano-convex lens 63 faces the function switching device 3, the double convex lens 61 and the plano-convex lens 63 are made of the same material, the refractive index of the concave lens 62 is higher than that of the double convex lens 61 and the plano-convex lens 63, and the double convex lens 61 and the plano-convex lens 63 are made of low dispersion materials such as fluorite, crown glass, AD glass, ED glass or UD glass.

[0116] As Figure 2 , Figures 10-12As shown, the optional dual optical detection device can further comprise a zoom light receiving device 5, which can be located between the function switching device 3 and the detection device 4. For example, the zoom light receiving device 5 is connected to the function switching device 3. For example, the zoom light receiving device 5 is connected to the first light outlet 313 of the second housing 31, and the light inlet of the zoom light receiving device 5 is in communication with the first light outlet 313. The receiving optical fiber 42 is connected to the light outlet of the zoom light receiving device 5.

[0117] The zoom light receiving device 5 can change the light receiving diameter of the light receiving side according to the light intensity of the fluorescence 8 or chemiluminescence. As mentioned above, in the present embodiment, when the content of the to-be-detected substance is small, if the zoom light receiving device 5 increases the light receiving diameter and thus the light receiving area, the detection device 4 can obtain a stronger light signal corresponding to the to-be-detected substance, and it can be determined that the to-be-detected substance is contained. If the zoom light receiving device 5 increases the light receiving diameter, and the detection device 4 still cannot detect the light signal corresponding to the to-be-detected substance, it indicates that the reaction solution does not contain the to-be-detected substance. Or, if the zoom light receiving device 5 increases the light receiving diameter, and the light signal still maintains the original intensity, it may be an interference signal, and the reaction solution does not contain the to-be-detected substance. When the zoom light receiving device 5 changes the light receiving diameter of the light receiving side according to the light intensity of the fluorescence 8 or chemiluminescence, it can be used to detect whether a specific to-be-detected substance is contained, and can also be used to detect the concentration of the to-be-detected substance. When calculating the concentration, the concentration of the to-be-detected substance can be calculated in combination with the light receiving diameter.

[0118] Of course, in other optional embodiments, the light receiving diameter of the zoom light receiving device 5 can be adjusted to the maximum before starting detection. When the light intensity that the detection device 4 can obtain is too large, the light receiving diameter of the zoom light receiving device 5 can be reduced. In this way, when the content of the to-be-detected substance is small, the detection device 4 can accurately detect the light signal.

[0119] Optionally, when the light intensity of the fluorescence 8 or chemiluminescence exceeds the minimum preset value, the zoom light receiving device 5 increases the light receiving diameter to determine whether the to-be-detected substance is contained.

[0120] When the light intensity of the fluorescence 8 or chemiluminescence exceeds the maximum preset value, the zoom light receiving device 5 reduces the light receiving diameter to avoid that the light intensity of the fluorescence 8 or chemiluminescence is too high, and the detection device 4 cannot detect the result.

[0121] In addition, in other alternative embodiments, for chemiluminescence immunoassay, the zooming light receiving device 5 adjusts its light receiving diameter according to the kind of the luminescent solution. The luminescent solution used for a specific to-be-detected substance is determined, and the efficiency of the specific luminescent solution to generate chemiluminescence is known. Therefore, when the zooming light receiving device 5 is adjusted according to the luminescent solution, the zooming light receiving device 5 can be adjusted before detection, that is, the light receiving diameter of the zooming light receiving device 5 is determined for a specific to-be-detected substance or luminescent solution. Thus, the relationship between the light intensity value detected by the detecting device 4 and the concentration of the to-be-detected substance can be determined in advance, and therefore, the calculation of the concentration of the to-be-detected substance is more convenient.

[0122] Specifically, if the efficiency of the luminescent solution to generate chemiluminescence is high, the zooming light receiving device 5 reduces its light receiving diameter; if the efficiency of the luminescent solution to generate chemiluminescence is low, the zooming light receiving device 5 increases its light receiving diameter. Thus, it is effectively avoided that, when the concentration of the to-be-detected substance is low, the concentration of the to-be-detected substance cannot be detected or it cannot be determined whether the to-be-detected substance is contained due to the low efficiency of the luminescent solution to generate chemiluminescence.

[0123] For fluorescence detection technology, the zooming light receiving device 5 adjusts its light receiving diameter according to the kind of the fluorescent substance.

[0124] The fluorescent substance used for a specific to-be-detected substance can be determined, and the fluorescence quantum yield (fluorescence quantum yield (Yf) is the ratio of the number of photons of the fluorescent light emitted by the fluorescent substance after absorbing light to the number of photons of the excitation light absorbed) of the specific fluorescent substance is known. Therefore, when the zooming light receiving device 5 is adjusted according to the fluorescent substance, the zooming light receiving device 5 can be adjusted before detection, that is, the light receiving diameter of the zooming light receiving device 5 is determined for a specific to-be-detected substance or fluorescent substance. Thus, the relationship between the light intensity value detected by the detecting device 4 and the concentration of the to-be-detected substance can be determined in advance, and therefore, the calculation of the concentration of the to-be-detected substance is more convenient.

[0125] Specifically, if the fluorescence quantum yield of the fluorescent substance is high, the zooming light receiving device 5 reduces its light receiving diameter; if the fluorescence quantum yield of the fluorescent substance is low, the zooming light receiving device 5 increases its light receiving diameter. Thus, it is effectively avoided that, when the concentration of the to-be-detected substance is low, the concentration of the to-be-detected substance cannot be detected or it cannot be determined whether the to-be-detected substance is contained due to the low fluorescence quantum yield of the fluorescent substance.

[0126] It can be understood that the light receiving diameter means that the light (fluorescent light 8 or chemiluminescence) incident to the zooming light receiving device 5 can enter the detecting device 4 within a certain diameter range centered on the central axis of the zooming light receiving device 5 at the incident end of the zooming light receiving device 5.

[0127] For example, the light receiving diameter of the zooming light receiving device 5 is 1 mm when the efficiency of the luminescent solution to generate chemiluminescence is high, and the light receiving diameter of the zooming light receiving device 5 is 2 mm when the efficiency of the luminescent solution to generate chemiluminescence is low. Figure 11aAs shown, at the incident end of the zoom light-collecting device 5, light within a diameter range of D1 can enter the zoom light-collecting device 5; as Figure 11b As shown, light within a diameter range of D2 can all enter the zoom light-receiving device 5; as Figure 11c Light within a diameter range of D3 can enter the zoom light receiving device 5, where D1 < D2 < D3.

[0128] like Figure 10 As shown in Figure 11, exemplarily, the zoomable light-collecting device 5 may include a first housing 51, a plurality of lenses 52, and a drive unit 53. The plurality of lenses 52 are disposed within the first housing 51 and spaced apart along the transmission directions of fluorescence 8 and chemiluminescence. The number of drive units 53 is at least one, and the drive unit 53 is connected to the lenses 52 to adjust the spacing between at least two adjacent lenses 52. The plurality of lenses 52 can focus fluorescence 8 and chemiluminescence onto the detection device 4. Optionally, the number of drive units 53 can be set as needed, as long as the focusing of fluorescence 8 and chemiluminescence onto the detection device 4 can be achieved by adjusting the spacing between the lenses 52. The drive unit 53 may be a linear motor.

[0129] like Figures 11a-11c As shown, exemplarily, the plurality of lenses 52 may include at least one lens group 521. The lens group 521 includes a first convex lens 524, a first concave lens 525, and a second convex lens 526 arranged sequentially. The first convex lens 524 is positioned near the detection device 4, and the second convex lens 526 is positioned near the function switching device 3. The distance between the first concave lens 525 and the first convex lens 524 and the second convex lens 526 is adjustable. Specifically, a driving member is connected to the first concave lens 525 to adjust the position of the first concave lens 525. Figures 11a-11c As shown, moving the first concave lens 525 toward the side where the first concave lens 525 is located, or moving the first concave lens 525 toward the side where the detection device 4 is located, can increase the light-gathering diameter of the zoom light-gathering device 5. Conversely, moving the first concave lens 525 toward the side where the second convex lens 526 is located, or moving the first concave lens 525 toward the side where the function switching device 3 is located, can decrease the light-gathering diameter of the zoom light-gathering device 5. That is, the first concave lens 525 gradually moves closer to the first convex lens 524, making D1 < D2 < D3.

[0130] The more lens groups 521 there are, the larger the adjustable range of the light-gathering diameter. For example, when the range of the light-gathering diameter that can be adjusted by one lens group 521 is too small, two or more lens groups 521 can be set.

[0131] In the two adjacent lens groups 521, the second convex lens 526 of one lens group 521 is the first convex lens 524 of the other lens group 521. The first concave lens 525 in each lens group 521 is connected with the driving member 53 to achieve synchronous adjustment. For example, one driving member 53 drives multiple first concave lenses 525 to move synchronously. The adjustment range of the light collecting diameter of the zoom light collecting device 5 is increased by the at least two lens groups 521, and the detection accuracy of the zoom light collecting device 5 is further improved.

[0132] For example, as shown in FIG. 5, the zoom light collecting device 5 is provided with two lens groups 521, which are located at the upper and lower sides of the middle of the zoom light collecting device 5. Figure 12 The lens group 521 located at the upper side of the middle includes a first convex lens 524, a first concave lens 525 and a second convex lens 526 arranged in sequence. Figure 12 The lens group 521 located at the lower side of the middle includes a first convex lens 524, a first concave lens 525 and a second convex lens 526 arranged in sequence. Figure 12 The second convex lens 526 of the lens group 521 located at the upper side of the middle is the same convex lens as the first convex lens 524 of the lens group 521 located at the lower side of the middle. Figure 12 The second convex lens 526 of the lens group 521 located at the upper side of the middle is the same convex lens as the first convex lens 524 of the lens group 521 located at the lower side of the middle. Figure 12 The second convex lens 526 of the lens group 521 located at the upper side of the middle is the same convex lens as the first convex lens 524 of the lens group 521 located at the lower side of the middle.

[0133] The number and arrangement of the multiple lenses 52 are not limited to the above, as long as the light collecting diameter of the zoom light collecting device 5 can be changed.

[0134] Optionally, the dual-optical detection device can further include a controller electrically connected with the function switching device 3, so that different light channels 321 can be selected according to the user's needs. The zoom light collecting device 5 and the detection device 4 can also be communicatively connected with the controller, so that the controller can control the zoom light collecting device 5 to adjust the light collecting diameter according to the type of luminescent liquid, the type of fluorescent substance or the light intensity detected by the detector 41. The user can also input the wavelength of the excitation light 7, the fluorescence 8 or the chemiluminescence to the controller, and the controller can switch the light channel 321 of the function switching device 3 according to the wavelength of the excitation light 7, the fluorescence 8 or the chemiluminescence.

[0135] For example, in the actual detection process, the controller can control the linear motor connected with the lens 52 to make fine adjustment through an algorithm according to the type of luminescent liquid, the type of fluorescent substance or the light intensity, to realize full-automatic zooming, so that the light in different diameter ranges can be gathered on the detector 41.

[0136] The dual-optical detection device can further comprise a second driving mechanism connected to the support 1, which drives the detection box 10 to move in a three-dimensional space. In one aspect, the detection box 10 can comprise a plurality of holes, and each hole is filled with a reaction solution independent of the others. The second driving mechanism drives the detection box 10 to move in a horizontal plane, so as to detect the reaction solution in each hole respectively. In another aspect, when the liquid level of the reaction solution in each hole changes, the second driving mechanism can also drive the detection box 10 to move in a vertical direction, so as to keep the distance between the liquid level of each reaction solution and the condenser 6 consistent, and further keep the position of the excitation light 7 entering the reaction solution consistent, thereby ensuring the accuracy of the detection result. The second driving mechanism can be any driving structure that can realize three-dimensional movement, such as a mechanical hand or a three-dimensional movement driving structure formed by sequentially connecting three linear driving members.

[0137] Optionally, the dual-optical detection device can further comprise a liquid level detection member, which can be connected to the controller. The liquid level detection member can be an image acquisition member or any structure that can detect the liquid level.

[0138] The liquid level detection member and the second driving mechanism can be electrically connected to the controller, so that the controller controls the second driving mechanism to raise or lower the height of the detection box 10 according to the detection result of the liquid level detection member.

[0139] Regarding the controller: In the present embodiment, the controller can be a centralized or distributed controller. For example, the controller can be a single microcontroller or a plurality of microcontrollers distributedly arranged. The microcontroller can run a control program, thereby controlling the above-mentioned components to realize their functions.

[0140] For example, the controller can be a single microcontroller or a plurality of microcontrollers distributedly arranged. Figure 5As shown, the dual-optical detection device can detect exosome-related indicators using a combination of chemiluminescence and fluorescence 8. When the detection personnel have a fluorescence detection requirement, the fluorescence detection state is entered, and the sample tube containing the reaction solution is placed in the corresponding hole of the detection box 10. The second driving mechanism of the dual-optical detection device moves the detection box 10 to the corresponding detection position. The reaction solution is aligned with the focal point of the apochromatic focusing device 6. At this time, the excitation light 7 emitted by the light source 21 is turned 90° downward into the sample tube through the incident optical fiber 22 and the dichroic mirror 3212. The reaction solution generates an excitation reaction and emits reaction light (i.e., fluorescence 8) upward. The reaction light is transmitted to the receiving optical fiber 42 and enters the detector 41. After processing, the relevant indicator values are obtained. When the detection personnel have a chemiluminescence detection requirement, the chemiluminescence detection state is entered, and the sample tube containing the reaction solution is placed in the corresponding hole of the detection box 10. The second driving mechanism of the dual-optical detection device moves the detection box 10 to the corresponding detection position. The reaction solution is aligned with the focal point of the apochromatic focusing device 6. At this time, the apochromatic focusing device 6 detects the light signal, and the reaction light is transmitted to the receiving optical fiber 42 and enters the detector 41. After processing, the relevant indicator values are obtained. The number and method of detection samples can be freely selected by the user, and multiple detections can be achieved with one loading.

[0141] Specifically, after the reaction solution absorbs the excitation light 7, it generates emission light, i.e., fluorescence 8. The emission light reaches the zoom light receiving device 5 through the apochromatic focusing device 6 and the channel switching device. The zoom light receiving device 5 amplifies and collects the self-luminescence into the receiving optical fiber 42. Finally, the fluorescence 8 signal is transmitted to the detector 41 through the optical fiber, and the target concentration in the exosome reaction solution is reflected.

[0142] When the detection system is set to the chemiluminescence detection state, Figure 5 the excitation light 7 is removed, and the fluorescence 8 is replaced by chemiluminescence. The function switching device 3 automatically switches to the light channel 321 corresponding to the chemiluminescence detection according to the user's instruction. The light channel 321 can include specific dichroic mirrors 3212 and filters 3211, or can not include dichroic mirrors 3212 and filters 3211, or can include one of dichroic mirrors 3212 and filters 3211, as long as it can transmit a specific wavelength of chemiluminescence. After receiving the chemiluminescence detection instruction, the dual-optical detection device detects whether the apochromatic focusing device 6 and the reaction solution surface are adapted, and then adjusts the height of the detection box 10 so that the chemiluminescence can be focused to the light channel 321 after passing through the apochromatic focusing device 6. After the focusing of the chemiluminescence passing through the apochromatic focusing device 6, the light channel 321 of the function switching device 3 transmits the chemiluminescence to the zoom light receiving device 5. After receiving the light energy signal, the zoom light receiving device 5 adjusts the displacement of the first concave lens 525 to amplify and collect the light signal into the receiving optical fiber 42. Finally, the reaction solution light signal is transmitted to the detector 41 through the receiving optical fiber 42.

[0143] In this embodiment, the zoom light collecting device 5, the function switching device 3 and the apochromatic focusing device 6 form a set of spatial light path detection system, which is matched with the light source 21 and the detector 41 to realize the detection of the chemiluminescence and fluorescence 8 optical signals, so as to ensure that the detection positions of the sample optical signals collected by different light channels 321 are consistent, and the zoom light collecting device 5 can automatically change the light collecting diameter to ensure the maximum light collecting range.

[0144] The dual optical detection device can perform dual-mode detection of multiple samples, can realize detection of chemiluminescence and fluorescence 8 signals respectively, is highly integrated, can realize two optical detection functions in one system, and has high automation degree and good compatibility and adaptability to samples.

[0145] The embodiment also provides a detection method, and the dual optical detection device performs the detection method, which can include the following steps:

[0146] S1, according to the detection mode required by the reaction liquid in the detection box 10, the function switching device 3 is switched to the corresponding fluorescence detection state or chemiluminescence detection state;

[0147] S2, the zoom light collecting device 5 changes the light collecting diameter according to the light intensity of the detected fluorescence 8 or chemiluminescence; or the zoom light collecting device 5 changes the light collecting diameter according to the type of the luminescent liquid; or the zoom light collecting device 5 changes the light collecting diameter according to the type of the fluorescent substance;

[0148] S3, the reaction liquid is detected.

[0149] The detection method can perform dual-mode detection to detect chemiluminescence and fluorescence 8 signals respectively. Through the zoom light collecting device 5, the light collecting diameter is changed according to the light intensity of the detected fluorescence 8 or chemiluminescence, or the type of the luminescent liquid or the type of the fluorescent substance, so that the detection device 4 can receive appropriate size of light signal whether for weak light signal or too strong light signal, thereby improving the accuracy of the detection result.

[0150] Optionally, before step S1, the detection box 10 can also be placed on the support 1.

[0151] Although the present application has been described in detail by general description, specific embodiments and tests, some modifications or improvements can be made on the basis of the present application, which is obvious to those skilled in the art. Therefore, these modifications or improvements made on the basis of not deviating from the spirit of the present application, all belong to the scope of the present application.

Claims

1. A dual optical detection device, characterized in that, include: A light source device (2) is used to emit excitation light (7); The function switching device (3) can switch between fluorescence detection state and chemiluminescence detection state; The detection device (4) is used to detect fluorescence (8) and chemiluminescence in the reaction solution; The zoom light-collecting device (5) is located between the function switching device (3) and the detection device (4), and the zoom light-collecting device (5) can change its light-collecting diameter.

2. The dual optical detection device according to claim 1, characterized in that, The zoom light-collecting device (5) can change its light-collecting diameter according to the intensity of the fluorescence (8) or the chemiluminescence; or The zoom light-collecting device (5) adjusts its light-collecting diameter according to the type of luminescent liquid; or The zoom light-collecting device (5) adjusts its light-collecting diameter according to the type of fluorescent material; or The zoom-focusing light-collecting device (5) can focus the fluorescence (8) or the chemiluminescence onto the detection device (4); or The zoom light-receiving device (5) is connected to the function switching device (3).

3. The dual optical detection device according to claim 2, characterized in that, When the intensity of the fluorescence (8) or the chemiluminescence exceeds a minimum preset value, the zoom light-collecting device (5) increases its light-collecting diameter; and / or when the intensity of the fluorescence (8) or the chemiluminescence exceeds a maximum preset value, the zoom light-collecting device (5) decreases its light-collecting diameter; or If the luminescent liquid has a high efficiency in generating the chemiluminescence, the zoom light-collecting device (5) reduces its light-collecting diameter; if the luminescent liquid has a low efficiency in generating the chemiluminescence, the zoom light-collecting device (5) increases its light-collecting diameter; or If the fluorescence quantum yield of the fluorescent material is high, the zoom light-collecting device (5) reduces its light-collecting diameter; If the fluorescence quantum yield of the fluorescent material is low, the zoom light-collecting device (5) increases its light-collecting diameter.

4. The dual optical detection device according to any one of claims 2-3, characterized in that, The zoom light-collecting device (5) includes a first housing (51), a plurality of lenses (52) and a driving member. The plurality of lenses (52) are disposed in the first housing (51) and spaced apart along the transmission direction of the fluorescence (8) and the chemiluminescence. The number of driving members is at least one. The driving member is connected to the lens (52) to adjust the spacing between at least two adjacent lenses (52). The plurality of lenses (52) can focus the fluorescence (8) and the chemiluminescence onto the detection device (4).

5. The dual optical detection device according to claim 4, characterized in that, The plurality of lenses (52) includes at least one lens group (521), the lens group (521) including a first convex lens (524), a first concave lens (525) and a second convex lens (526) arranged in sequence, wherein the first convex lens (524) is disposed close to the detection device (4) and the second convex lens (526) is disposed close to the function switching device (3), and the distance between the first concave lens (525) and the first convex lens (524) and the second convex lens (526) is adjustable.

6. The dual optical detection device according to claim 5, characterized in that, At least two sets of the lens groups (521) are provided, wherein in two adjacent sets of the lens groups (521), the second convex lens (526) of one set of the lens groups (521) is the first convex lens (524) of the other set of the lens groups (521).

7. The dual optical detection device according to any one of claims 2-3, characterized in that, The function switching device (3) includes at least two optical channels (321) and a working position; Each of the optical channels (321) can be located at the working position so that at least one of the optical channels (321) can transmit the chemiluminescence to the detection device (4), so that the function switching device (3) is in the chemiluminescence detection state, and so that at least one of the optical channels (321) can transmit the excitation light (7) of a preset wavelength to the reaction solution and transmit the fluorescence (8) to the detection device (4), so that the function switching device (3) is in the fluorescence detection state.

8. The dual optical detection device according to claim 7, characterized in that, At least one of the optical channels (321) includes a filter (3211) and a dichroic mirror (3212). The filter (3211) allows excitation light (7) of a preset wavelength to enter the optical channel (321), and the dichroic mirror (3212) allows fluorescence (8) of a preset wavelength to pass through, so that the fluorescence (8) enters the detection device (4).

9. The dual optical detection device according to claim 8, characterized in that, The function switching device (3) includes a second housing (31) and a mounting bracket (32). The mounting bracket (32) includes at least two optical channels (321). The mounting bracket (32) is movably disposed on the second housing (31) so that each optical channel (321) can be located at the working position. The optical channel (321) located at the working position can transmit the excitation light (7) and the fluorescence (8) of a preset wavelength or can transmit the chemiluminescence.

10. The dual optical detection device according to claim 9, characterized in that, The mounting bracket (32) is rotatably disposed within the second housing (31), and the at least two optical channels (321) are spaced apart along the rotation center of the mounting bracket (32); and / or The second housing (31) includes a first light inlet (311), a first light outlet (312), and a first light outlet (313). The optical channel (321) includes a second light inlet (3213), a second light outlet (3234), and a second light outlet (3235). When the optical channel (321) is in the working position, the first light inlet (311) and the second light inlet (3213) are directly opposite each other. The first light inlet (311) and the second light outlet (3235) are directly opposite each other. The excitation light (7) enters the second housing (31) through the first light inlet (311). The excitation light (7) of the preset wavelength is emitted out of the second housing (31) through the first light inlet / outlet (312). The fluorescence (8) and the chemiluminescence enter the second housing (31) through the first light inlet / outlet (312) and are emitted out of the second housing (31) through the first light outlet (313).

11. The dual optical detection device according to claim 10, characterized in that, The mounting frame (32) includes a frame (322) which is rotatably connected to the second housing (31). The optical channel (321) also includes an optical tube (3236) which is detachably connected to the frame (322). The at least one optical tube (3236) is provided with the filter (3211) and the dichroic mirror (3212).

12. The dual optical detection device according to claim 11, characterized in that, The function switching device (3) further includes a first drive mechanism (33), which is connected to the second housing (31) and the mounting bracket (32) and drives the mounting bracket (32) to rotate.

13. The dual optical detection device according to claim 10, characterized in that, The second light outlet (3235) and the second light inlet / outlet (3234) are located on the same straight line, and the second light inlet (3213) is perpendicular to the second light outlet (3235); The filter (3211) is disposed at the second light inlet (3213), and the dichroic mirror (3212) is disposed at the light channel (321), and is at a 45° angle to both the second light inlet (3213) and the second light outlet (3234).

14. The dual optical detection device according to claim 1, characterized in that, The light source device (2) includes a light source (21) and an incident optical fiber (22), the incident optical fiber (22) being used to transmit the excitation light (7) emitted by the light source (21) to the function switching device (3); and / or The detection device (4) includes a detector (41) and a receiving optical fiber (42). The receiving optical fiber (42) is used to transmit the fluorescence (8) and chemiluminescence output by the function switching device (3) to the detector (41). The detector (41) is used to detect the fluorescence (8) and the chemiluminescence; and / or The dual optical detection device also includes a second driving mechanism, which drives the detection box (10) containing the reaction liquid to move in three-dimensional space.

15. The dual optical detection device according to claim 1, characterized in that, The dual optical detection device also includes an apochromatic focusing device (6), which is located downstream of the function switching device (3). The apochromatic focusing device (6) is used to focus the beams formed by the excitation light (7) of different wavelengths onto the same test site.

16. A detection method, characterized in that, The dual-optical detection device according to any one of claims 1-15 performs the detection method, the detection method comprising the following steps: According to the detection method required by the reaction liquid in the detection box (10), switch the function switching device (3) to the corresponding fluorescence detection state or chemiluminescence detection state; The zoom light-collecting device (5) changes its light-collecting diameter according to the intensity of the detected fluorescence (8) or chemiluminescence; or the zoom light-collecting device (5) changes its light-collecting diameter according to the type of luminescent liquid; or the zoom light-collecting device (5) changes its light-collecting diameter according to the type of fluorescent substance. The reaction solution was tested.

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