Preparation method and application of oil-soluble composition for relieving, resisting inflammation and scavenging free radicals

Abstract

The invention relates to the technical field of raw materials of skin care products, and particularly discloses a preparation method and application of an oil-soluble composition for relieving, resisting inflammation and scavenging free radicals. The preparation method of the oil-soluble composition for relieving, resisting inflammation and scavenging free radicals comprises the following steps: step 1) mixing stephania tetrandra, oenothera biennis, hyssopus officinalis, centella asiatica and artemisia apiacea to obtain a composite plant raw material; 2) mixing the composite plant raw material with an extraction solvent, soaking, extracting, filtering, and taking filtrate; 3, the filtrate is subjected to molecular distillation to separate and extract the solvent, distillate is collected, and the soothing, anti-inflammatory and free radical scavenging oil-soluble composition is obtained.The soothing, anti-inflammatory and free radical scavenging oil-soluble composition has the advantages that the free radical scavenging effect is further improved, and a more remarkable anti-aging effect is provided for skin care products.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of skin care raw materials, in particular to a soothing, anti-inflammatory and free radical scavenging oil-soluble composition preparation method and application. BACKGROUND

[0002] Skin care products are mainly used for skin care. By adding ingredients with specific effects in skin care products, the skin care products can be endowed with the effect of improving the skin. With the development of science and technology and the continuous improvement of consumers' safety requirements for skin care products, more green and safe plant extracts have become one of the main skin care raw materials.

[0003] In existing skin care products, the corresponding skin care effect of the skin care products is mainly improved by adding plant extracts with specific effects. However, the effect of a single extract is usually general, especially the free radical scavenging effect of plant extracts. It is difficult to achieve a very high free radical scavenging effect by using a single extract. Although there are studies on the compounding of multiple extracts in the prior art to improve the skin care effect, the current composite plant extracts are still general in the free radical scavenging effect, and it is difficult to meet the expectations of consumers for the anti-aging effect of skin care products. Therefore, there is still room for improvement. SUMMARY

[0004] In order to further improve the free radical scavenging effect and provide more significant anti-aging effect for skin care products, the present application provides a soothing, anti-inflammatory and free radical scavenging oil-soluble composition preparation method and application.

[0005] In a first aspect, the present application provides a soothing, anti-inflammatory and free radical scavenging oil-soluble composition preparation method, which adopts the following technical solution: A soothing, anti-inflammatory and free radical scavenging oil-soluble composition preparation method, characterized in that it comprises the following steps: Step 1), mixing Radix Stephaniae Tetrandrae, evening primrose, hyssop, Centella asiatica and Artemisia annua to obtain a composite plant raw material; Step 2), mixing the composite plant raw material with an extraction solvent, soaking and extracting, filtering, and taking the filtrate; Step 3), subjecting the filtrate to molecular distillation to separate the extraction solvent, collecting the distillate, and obtaining a soothing, anti-inflammatory and free radical scavenging oil-soluble composition.

[0006] By adopting the technical scheme, the powder aconite, evening primrose, hyssop, centella asiatica and artemisia annua are compounded as the extraction raw materials, and after the compounding extraction, the composition obtained can play a very significant free radical scavenging effect under the mutual cooperation of various oil-soluble active ingredients, and has good soothing and anti-inflammatory effects, and can also have an anti-glycation effect, can comprehensively repair the skin, and can play a very significant anti-aging effect, and the skin can be well restored to be young and healthy, and the needs of consumers can be well met.

[0007] Preferably, in the step 1), the mass ratio of the powder aconite, evening primrose, hyssop, centella asiatica and artemisia annua is 1.9-2.1:1.9-2.1:0.4-0.6:0.7-0.9:0.6-0.8.

[0008] By adopting the technical scheme, the mass ratio of the powder aconite, evening primrose, hyssop, centella asiatica and artemisia annua is specifically selected, so that the active ingredients are better matched in a specific ratio, the synergistic effect is better, and the soothing, anti-inflammatory, free radical scavenging and anti-glycation effects are more significant.

[0009] Preferably, in the step 2), the extraction solvent is a compound of caprylic acid / capric acid triglyceride and n-hexane.

[0010] By adopting the technical scheme, the compound of caprylic acid / capric acid triglyceride and n-hexane is specifically selected, so that the solubility of the extraction solvent to the oil-soluble active ingredients in the raw materials is higher, the active ingredients can be better extracted into the extraction solvent, the extraction effect is better, and the skin care effect of the composition prepared is better.

[0011] Preferably, in the extraction solvent, the mass ratio of caprylic acid / capric acid triglyceride and n-hexane is 1-2:0.8-1.

[0012] By adopting the technical scheme, the mass ratio of caprylic acid / capric acid triglyceride and n-hexane is specifically selected, so that the extraction effect of the active ingredients in the raw materials can be better improved, and the product quality is better.

[0013] Preferably, in the step 2), the mass ratio of the compound plant raw material to the extraction solvent is 1:5-6.

[0014] By adopting the technical scheme, the mass ratio of the compound plant raw material to the extraction solvent is specifically selected, so that the extraction effect is better, the waste of the extraction solvent is reduced, the energy consumption of the subsequent steps is reduced, and the economic value is higher.

[0015] Preferably, in the step 3), first, primary distillation is carried out at ≤0.1 Pa and 30-50°C, and the distillation is carried out until no distillation liquid flows out, then the primary distillation liquid is removed, and then the temperature is raised to 55-70°C for secondary distillation, and the secondary distillation liquid is collected, which is the soothing, anti-inflammatory, and free radical scavenging oil-soluble composition.

[0016] By adopting the above technical solution, the low-boiling-point solvent is removed through primary distillation, and the target product is separated through secondary distillation, so that the soothing, anti-inflammatory, and free radical scavenging oil-soluble composition obtained has better quality and better skin care effect.

[0017] Preferably, in the step 2), after the composite plant raw material is mixed with the extraction solvent, the mixture is soaked and extracted at 30-50°C for 28-32h.

[0018] By adopting the above technical solution, the extraction is more sufficient, the waste of raw materials is reduced, the soaking and extraction at low temperature reduce the damage to active ingredients, the efficacy of active ingredients can be well maintained, and the product quality is better.

[0019] Preferably, in the step 1), the Radix Stephaniae Tetrandrae, evening primrose, hyssop, Centella asiatica, and Artemisia annua are crushed and then mixed, and the particle size of the crushed Radix Stephaniae Tetrandrae, evening primrose, hyssop, Centella asiatica, and Artemisia annua is 0.1-0.2mm.

[0020] By adopting the above technical solution, the raw materials are crushed, which can better cooperate with the extraction solvent at a special particle size, so that the extraction effect is more significant, the extraction is more sufficient, and the product quality is better.

[0021] In a second aspect, the application provides an application of a soothing, anti-inflammatory, and free radical scavenging oil-soluble composition, which adopts the following technical solution: The application of the soothing, anti-inflammatory, and free radical scavenging oil-soluble composition is applied to skin care products and cosmetic formulations to prepare skin care products and cosmetics with soothing, anti-inflammatory, and free radical scavenging effects.

[0022] By adopting the above technical solution, the prepared skin care products and cosmetics have a significant free radical scavenging effect, and also have good soothing, anti-inflammatory, and anti-glycation effects, can comprehensively care for the skin, have a significant anti-aging and repair effect on the skin, and well meet the needs of consumers.

[0023] In summary, the application has the following beneficial effects: 1、Since the application takes the combination of radix stephaniae tetrandrae, evening primrose, hyssop, centella asiatica and artemisia as the extraction raw material, through the extraction of the combination, the composition obtained can play a very significant free radical scavenging effect under the mutual cooperation of various oil-soluble active ingredients, and also has good soothing and anti-inflammatory effects, and can also play an anti-glycation effect, can comprehensively repair the skin, and can play a very significant anti-aging effect, the skin can be restored to youth and health, and the needs of consumers can be well met.

[0024] 2、In the application, the mass ratio of radix stephaniae tetrandrae, evening primrose, hyssop, centella asiatica and artemisia is preferably selected, so that the active ingredients are better matched in a specific ratio, the synergistic effect is better, and the soothing, anti-inflammatory, free radical scavenging and anti-glycation effects are more significant.

[0025] 3、In the application, the combination of caprylic / capric triglyceride and n-hexane is preferably selected, so that the solubility of the extraction solvent to the oil-soluble active ingredients in the raw material is higher, the active ingredients can be better extracted into the extraction solvent, the extraction effect is better, and thus the skin care effect of the prepared composition is better. DETAILED DESCRIPTION

[0026] The application will be further described in detail below in combination with examples. Example 1

[0027] A soothing, anti-inflammatory and free radical scavenging oil-soluble composition preparation method comprises the following steps: Step 1), radix stephaniae tetrandrae, evening primrose, hyssop, centella asiatica and artemisia are respectively crushed by a crushing device, sieved, and 0.1-0.2mm powder raw materials are obtained. 1.9kg of radix stephaniae tetrandrae powder, 1.9kg of evening primrose powder, 0.4kg of hyssop powder, 0.7kg of centella asiatica powder and 0.6kg of artemisia powder are weighed and put into a stirring kettle, the stirring speed is 30r / min, and stirring is performed for 5min, so as to obtain a composite plant raw material.

[0028] Step 2), 27.5kg of extraction solvent is injected into the stirring kettle containing the composite plant raw material, the stirring speed is 30r / min, continuous stirring is performed, heating is performed to 40℃, and extraction is performed for 28h. After filtration, the filter residue is discarded, and the filtrate is taken; wherein the extraction solvent is prepared by compounding caprylic / capric triglyceride and n-hexane at a mass ratio of 1:0.8.

[0029] Step 3), the filtrate is injected into a molecular distillation device, first-level distillation is performed at 0.1Pa and 30℃, the distillation is performed until no distillation liquid flows out, the distillation liquid obtained by first-level distillation is removed, then the pressure is kept at 0.1Pa, and the temperature is raised to 55℃ for second-level distillation, and the distillation liquid obtained by second-level distillation is collected, which is a soothing, anti-inflammatory and free radical scavenging oil-soluble composition.

[0030] Radix Stephaniae Tetrandrae, evening primrose, hyssop, Centella asiatica, Artemisia annua are all from the market, dry goods.

[0031] Caprylic / capric triglyceride is purchased from Guangzhou Huicun Supply Chain Management Co., Ltd.

[0032] N-hexane is purchased from Guangzhou Qiuyuehua Trading Co., Ltd. Example 2

[0033] A soothing, anti-inflammatory, free radical scavenging oil-soluble composition preparation method, comprising the following steps: Step 1), Radix Stephaniae Tetrandrae, evening primrose, hyssop, Centella asiatica, Artemisia annua are crushed by crushing equipment, sieved, and powder raw materials with a particle size of 0.1-0.2mm are obtained. 2kg of Radix Stephaniae Tetrandrae powder, 2kg of evening primrose powder, 0.5kg of hyssop powder, 0.8kg of Centella asiatica powder, and 0.7kg of Artemisia annua powder are weighed and put into a stirring kettle, the stirring speed is 30r / min, and stirring for 5min, the mixture is uniformly mixed, and the composite plant raw material is obtained.

[0034] Step 2), 33kg of extraction solvent is injected into the stirring kettle containing the composite plant raw material, the stirring speed is 30r / min, continuous stirring, heating to 42℃, extraction for 30h, filtration, discarding the filter residue, and taking the filtrate; wherein the extraction solvent is compounded by caprylic / capric triglyceride and n-hexane in a mass ratio of 1.5:0.9.

[0035] Step 3), the filtrate is injected into a molecular distillation device, first level distillation is carried out at 0.1Pa and 40℃, distillation is carried out until no distillation liquid flows out, the distillation liquid obtained by first level distillation is removed, then 0.1Pa is maintained, and the temperature is raised to 62.5℃ for secondary distillation, the distillation liquid obtained by secondary distillation is collected, and the soothing, anti-inflammatory, free radical scavenging oil-soluble composition is obtained.

[0036] Radix Stephaniae Tetrandrae, evening primrose, hyssop, Centella asiatica, Artemisia annua are all from the market, dry goods.

[0037] Caprylic / capric triglyceride is purchased from Guangzhou Huicun Supply Chain Management Co., Ltd.

[0038] N-hexane is purchased from Guangzhou Qiuyuehua Trading Co., Ltd. Example 3

[0039] A soothing, anti-inflammatory, free radical scavenging oil-soluble composition preparation method, comprising the following steps: Step 1), the powder of Stephania tetrandra, evening primrose, hyssop, Centella asiatica and Artemisia annua were crushed by crushing equipment, sieved, and powder raw materials with a particle size of 0.1-0.2 mm were obtained. 2.1 kg of Stephania tetrandra powder, 2.1 kg of evening primrose powder, 0.6 kg of hyssop powder, 0.9 kg of Centella asiatica powder and 0.8 kg of Artemisia annua powder were weighed and put into a stirring kettle, stirred at a speed of 30 r / min for 5 min, and uniformly mixed to obtain a composite plant raw material.

[0040] Step 2), 39 kg of extraction solvent was injected into the stirring kettle containing the composite plant raw material, and stirred at a speed of 30 r / min. The temperature was heated to 45°C, and the extraction was carried out for 32 h. After filtration, the filter residue was discarded, and the filtrate was obtained; wherein the extraction solvent was composed of caprylic acid / capric acid triglyceride and n-hexane at a mass ratio of 2:1.

[0041] Step 3), the filtrate was injected into a molecular distillation device. First-level distillation was carried out at 0.1 Pa and 50°C. The distillation was carried out until no distillation liquid flowed out. The distillation liquid obtained by first-level distillation was removed. Then, the second-level distillation was carried out at 0.1 Pa and 70°C. The distillation liquid obtained by second-level distillation was collected, which was a soothing, anti-inflammatory and free radical scavenging oil-soluble composition.

[0042] Stephania tetrandra, evening primrose, hyssop, Centella asiatica and Artemisia annua were all obtained from the market, and were dry products.

[0043] Caprylic acid / capric acid triglyceride was purchased from Guangzhou Huicun Supply Chain Management Co., Ltd.

[0044] N-hexane was purchased from Guangzhou Qiuyuehua Trading Co., Ltd.

[0045] Comparative Example 1 A method for preparing a soothing, anti-inflammatory and free radical scavenging oil-soluble composition, compared with Example 2, only differs in that: Grape seeds were used to replace Stephania tetrandra in an equal amount.

[0046] Grape seeds were obtained from the market, and were dry products.

[0047] Comparative Example 2 A method for preparing a soothing, anti-inflammatory and free radical scavenging oil-soluble composition, compared with Example 2, only differs in that: Almonds were used to replace evening primrose in an equal amount.

[0048] Almonds were obtained from the market, and were dry products.

[0049] Comparative Example 3 A method for preparing a soothing, anti-inflammatory and free radical scavenging oil-soluble composition, compared with Example 2, only differs in that: Tea seeds were used to replace hyssop in an equal amount.

[0050] Tea seed is derived from commercially available, dry product.

[0051] Comparative Example 4 A method for preparing a soothing, anti-inflammatory, free radical scavenging oil-soluble composition, compared with Example 2, the only difference is that: Blackberry seeds are used to replace Centella asiatica in equal amounts.

[0052] Blackberry seeds are derived from commercially available, dry product.

[0053] Comparative Example 5 A method for preparing a soothing, anti-inflammatory, free radical scavenging oil-soluble composition, compared with Example 2, the only difference is that: Safflower seeds are used to replace Artemisia annua in equal amounts.

[0054] Safflower seeds are derived from commercially available, dry product.

[0055] Comparative Example 6 A method for preparing a soothing, anti-inflammatory, free radical scavenging oil-soluble composition, compared with Example 2, the only difference is that: Grape seeds are used to replace Stephania tetrandra in equal amounts, almonds are used to replace evening primrose in equal amounts, tea seeds are used to replace Hyptis suaveolens in equal amounts, blackberry seeds are used to replace Centella asiatica in equal amounts, and safflower seeds are used to replace Artemisia annua in equal amounts.

[0056] Grape seeds are derived from commercially available, dry product.

[0057] Almonds are derived from commercially available, dry product.

[0058] Tea seeds are derived from commercially available, dry product.

[0059] Blackberry seeds are derived from commercially available, dry product.

[0060] Safflower seeds are derived from commercially available, dry product.

[0061] Experiment 1 According to T / JSRH 003-2023 "Cosmetic raw material anti-allergic activity test Hyaluronidase inhibition method", the hyaluronidase inhibition rate (%) of the test sample prepared from the soothing, anti-inflammatory, free radical scavenging oil-soluble composition of each example and comparative example is detected.

[0062] The test sample: 1g of soothing, anti-inflammatory, free radical scavenging oil-soluble composition, 99g of jojoba oil are mixed uniformly to obtain the test sample.

[0063] Experiment 2 In vitro anti-glycation experiment A BSA-glucose simulation reaction system was established, and a positive control (aminoguanidine sulfate) and a blank control group were set up. The fluorescence intensity of the sample was determined by a multifunctional enzyme marker, and the inhibition rate of the sample on the fluorescence AGES was calculated.

[0064] Sample: The sample to be tested prepared from the soothing, anti-inflammatory, free radical scavenging oil-soluble composition of each example and the comparative example, aminoguanidine sulfate (AG).

[0065] Reagent consumables: black bottom transparent 96-well plate, dipotassium phosphate, potassium dihydrogen phosphate, bovine serum albumin (BSA), glucose, deionized water.

[0066] Instrument equipment: multifunctional enzyme label instrument (Infinite M200 Pro).

[0067] Experimental method: Under sterile conditions, 2.0 mL of 20 mg / mL bovine serum albumin solution and 2.0 mL of 0.4 M glucose solution were added to centrifuge tubes respectively and mixed uniformly. The following test groups were set up for the experiment, and different treatments were carried out for each group, as follows: Blank control group: adding phosphate buffer solution without treatment Experimental group: adding the sample to be tested prepared from the soothing, anti-inflammatory, free radical scavenging oil-soluble composition of each example and the comparative example Positive control group: 2.0 ml of 0.5 mg / mL (0.05%) aminoguanidine sulfate (AG) solution After incubation at 37°C for 40 days in the dark, the fluorescence intensity of advanced glycation end products (AGEs) in each test group was measured using a multifunctional enzyme label instrument at an excitation wavelength of 370 nm and an emission wavelength of 440 nm, and the calculation was carried out according to the following formula: AGEs inhibition rate: IR% = [1-(Aa-Ab) / (Ac-Ad)]*100% Wherein: Aa is the average fluorescence intensity of the experimental group and the positive control group, Ab is the average fluorescence intensity of the sample background hole, Ac is the average fluorescence intensity of the control group BSA and glucose, and Aa is the average fluorescence intensity of BSA.

[0068] Sample to be tested: 1 g of soothing, anti-inflammatory, free radical scavenging oil-soluble composition, 99 g of jojoba oil were mixed uniformly to obtain the sample to be tested.

[0069] Experiment 3 The DPPH free radical scavenging rate (%) of the sample to be tested prepared from the soothing, anti-inflammatory, free radical scavenging oil-soluble composition of each example and the comparative example was detected according to T / SHRH 006-2018 "Cosmetics- Free radical (DPPH) scavenging experimental method".

[0070] Sample to be tested: 1 g of soothing, anti-inflammatory, free radical scavenging oil-soluble composition, 99 g of jojoba oil were mixed uniformly to obtain the sample to be tested.

[0071] The specific experimental data of experiments 1-3 are shown in Table 1.

[0072] Table 1

[0073] According to the data comparison of each embodiment and the comparative example in Table 1, each embodiment and the comparative example has good DPPH free radical scavenging rate, and the DPPH free radical scavenging rate of each embodiment is higher. It can be seen that each embodiment can play a more significant effect of scavenging free radicals, and the soothing and anti-glycation effects of each embodiment are significantly higher than those of the comparative example. It can be seen that each embodiment can not only quickly scavenge free radicals, but also relieve skin allergic phenomena, make the skin healthier, and effectively resist glycation to reduce skin aging caused by glycation. Through multi-aspect cooperation in nursing the skin, the skin anti-aging effect is significantly improved.

[0074] The specific embodiments are merely an explanation of the present application, and are not a limitation of the present application. Those skilled in the art can make modifications to the embodiments without creative contribution after reading the present specification, but as long as the modifications are within the scope of the claims of the present application, they are protected by the patent law.

Claims

1. A method of making an oil-soluble composition for soothing, anti-inflammatory, free-radical scavenging, characterized in that: The method comprises the following steps: Step 1), mixing the Stephania tetrandra S. Moore, Oenothera biennis Linn, Hyptis suaveolens Poit, Centella asiatica Linn, Artemisia annua Linn to obtain a composite plant raw material; Step 2), mixing the composite plant raw material with an extraction solvent, soaking and extracting, filtering, and taking the filtrate; Step 3), subjecting the filtrate to molecular distillation to separate the extraction solvent, and collecting the distillate to obtain an oil-soluble composition with soothing, anti-inflammatory, and free radical scavenging effects.

2. A process for the preparation of an oil-soluble composition for soothing, anti-inflammatory, free radical scavenging purposes according to claim 1, characterized in that: In step 1), the mass ratio of the Stephania tetrandra S. Moore, Oenothera biennis Linn, Hyptis suaveolens Poit, Centella asiatica Linn, and Artemisia annua Linn is 1.9-2.1:1.9-2.1:0.4-0.6:0.7-0.9:0.6-0.

8.

3. A process for the preparation of an oil-soluble composition for soothing, anti-inflammatory, free radical scavenging purposes according to claim 2, characterized in that: In step 2), the extraction solvent is a mixture of caprylic acid / capric acid triglyceride and n-hexane.

4. A process for the preparation of an oil-soluble composition for soothing, anti-inflammatory, free radical scavenging purposes according to claim 3, characterized in that: In the extraction solvent, the mass ratio of caprylic acid / capric acid triglyceride and n-hexane is 1-2:0.8-1.

5. A process for the preparation of an oil-soluble composition for soothing, anti-inflammatory, free radical scavenging purposes according to claim 4, characterized in that: In step 2), the mass ratio of the composite plant raw material to the extraction solvent is 1:5-6.

6. A method of making an oil-soluble composition for soothing, anti-inflammatory, free radical scavenging purposes according to claim 1, characterized in that: In step 3), first-level distillation is performed at ≤0.1 Pa and 30-50°C, and the distillate obtained in the first-level distillation is removed after distillation to no distillate flows out, and then the temperature is raised to 55-70°C for second-level distillation, and the distillate obtained in the second-level distillation is collected to obtain the oil-soluble composition with soothing, anti-inflammatory, and free radical scavenging effects.

7. A process for the preparation of an oil-soluble composition for soothing, anti-inflammatory, free radical scavenging purposes according to claim 1, characterized in that: In step 2), after mixing the composite plant raw material with the extraction solvent, soaking and extracting are performed at 30-50°C for 28-32 h.

8. A method of making a soothing, anti-inflammatory, free-radical scavenging oil-soluble composition according to claim 1, characterized by: In step 1), the Stephania tetrandra S. Moore, Oenothera biennis Linn, Hyptis suaveolens Poit, Centella asiatica Linn, and Artemisia annua Linn are crushed before mixing, and the particle size of the crushed Stephania tetrandra S. Moore, Oenothera biennis Linn, Hyptis suaveolens Poit, Centella asiatica Linn, and Artemisia annua Linn is 0.1-0.2 mm.

9. Use of an oil-soluble composition for soothing, anti-inflammatory, free-radical scavenging according to any one of claims 1 to 8, characterized in that: The oil-soluble composition with soothing, anti-inflammatory, and free radical scavenging effects is applied to skin care products and cosmetic formulations to prepare skin care products and cosmetics with soothing, anti-inflammatory, and free radical scavenging effects.

Images